GABPI cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	GABPI
Entrez Gene ID	33516
Full Name	beta4GalNAcTB pilot
Synonyms	CG17257,Dmel\CG17257
General protein information	Preferred Names beta4GalNAcTB pilot Names CG17257 gene product from transcript CG17257-RC CG17257-PA CG17257-PB CG17257-PC CG17257-PD GABPI-PA GABPI-PB GABPI-PC GABPI-PD
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 23C5-23C5

mRNA and Protein(s)

mRNA	Protein	Name
NM_134897.5	NP_608741.1	beta4GalNAcTB pilot, isoform B
NM_164522.3	NP_722869.1	beta4GalNAcTB pilot, isoform A
NM_001298645.1	NP_001285574.1	beta4GalNAcTB pilot, isoform D
NM_001298644.1	NP_001285573.1	beta4GalNAcTB pilot, isoform C

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ZDHHC23	NP_001229986.1
	ZDHHC23	XP_001100427.1
Bos taurus (cattle)	ZDHHC23	NP_001179243.1
Mus musculus (house mouse)	Zdhhc23	NP_001007461.1
Rattus norvegicus (Norway rat)	Zdhhc23	NP_998792.2
Danio rerio (zebrafish)	zdhhc23b	NP_001003757.1
Drosophila melanogaster (fruit fly)	GABPI	NP_608741.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008264	XP_317208.4
Homo sapiens (human)	ZDHHC23	NP_775841.2
Xenopus tropicalis (tropical clawed frog)	zdhhc23	XP_002939692.1
Canis lupus familiaris (dog)	ZDHHC23	XP_849643.1
Gallus gallus (chicken)	ZDHHC23	NP_001012837.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Lumenal loop regions are insect-conserved and essential for GABPI-b4GalNAcTB interaction.
Title: "Add-on" domains of Drosophila ?1,4-N-acetylgalactosaminyltransferase B in the stem region and its pilot protein.

beta4GalNAcTB pilot (GABPI) is required for ER export and activity of beta4GalNAcTB.
Title: Golgi targeting of Drosophila melanogaster beta4GalNAcTB requires a DHHC protein family-related protein as a pilot.

The following GABPI gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GABPI cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa13592	NM_164522.3 Latest version!	Drosophila melanogaster beta4GalNAcTB pilot (GABPI), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa13592	NM_134897.5 Latest version!	Drosophila melanogaster beta4GalNAcTB pilot (GABPI), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa21582	NM_001298644.1 Latest version!	Drosophila melanogaster beta4GalNAcTB pilot (GABPI), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa21582	NM_001298645.1 Latest version!	Drosophila melanogaster beta4GalNAcTB pilot (GABPI), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GABPI ( NM_134897.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_608741.1 Drosophila melanogaster beta4GalNAcTB pilot (GABPI), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa13592
Clone ID Related Accession (Same CDS sequence)	NM_134897.5 , NM_164522.3
Accession Version	NM_134897.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1263bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	beta4GalNAcTB pilot, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_134897.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCTTGA TGACAGCCGG CACCTCCAAT TTTCTTTCTG CAGCCTGGAG ATCAGAGGCT 
GAGGAGTCCA AGAGTAAGGA GAACGGGAGT CTCTGCTGCT GCGAGTACGT GGCTGAGGAC 
AGTCGAGAGC GATCCCACAT CCTAGGATGC TGTTGTAACT GCGTGGACTT CGACTTAGTT 
TGCACAAGAC TTGTCACCTG CCGAGCCATT GATCGAAGGA ACATCGACGG CATGCTCATC 
GCCTTCCAGG ATAGACTGCG CTTGCCATGG CGCGGAGGAG CCAAGCGAAT CTCGCCCGCA 
GCCGTTGCTC CCGCCTTCAT TGTCCCCCTC ATGCTGGGAT TGGCCACTCT GAACTCAAAG 
ACCGCCATAG TGCTGATGCT CACGCTGGTG GGCTTCACCA TCTGGGGCAT GGAGCTGGCT 
AAGCGGACCG CCACGCGGAC AAACTTCTTT TTGAGCTGGC TGGTCTTCTC GGTGTTCTAC 
ATGATCATCA TATTTGAGTT TCAGGTTCCT CTGCTGGAGC TGGCTCCGGA GGAGAACTAC 
GCGTTAATGT TCTTCTCCTG TGCCGCTTTG TACTGCCTCT ATTCCGCCAA GGCACTGTCG 
CCCTTGAATC TTGTTTCTGC TCAATACGGA ACCACGCCCA AGGATGAGCT GCCTGGGATC 
GCCGAGGCTT CGAGTGGCGA GGAGCAGGCA GAGGCCCAGA CCACACTCCA GATGGAGAGT 
GTGCTGAGTT TGGATGATGA CGAGGTGGGG GATATGGACA CTGCTGAGCG ATCTGGCTTG 
ATGCACGGTC AGCCCAATAT CTGCGAGATC TGCCGGAAGG TTACTCCAAG GCGGGCATAC 
CACTGTCCCG TCTGCGGAAC CTGCGTGAAG AGGCGCGATC ATCACAGCTA CTGGCTAAAC 
TGCTGCATTG GCGAGCGGAA CTACGTGTGG TACATTGTTG GACTGGCCCT GTCGGAGATC 
GCTCTGCTGC TCGGCGCAAA CTTGACCCTC ACTTCCATTT GCCATCCGTT TATGGTGGTG 
CGACCGCTTG GTTATCCAGT TCTTTTGCCC GACGACTGCA GTGAGGTGTT CGAAGGCTTC 
GACCTCGGAA TCTCCTTTGT GGTGGCTTGC TACGCTCTGC TCATTTCTTC CTACATAGCC 
TTTATACTCG CTAGACAGGC TTACTTGTGG TGGAAGGGAT CCACCTTGCA CGAATACAAG 
CGCACCTCCA ACGCCGCAGG ACGCAACAGA ATCTGGAGCA ACTGGCGAGC CATCTTAAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_608741.1
CDS	128..1390
Translation	MFLMTAGTSNFLSAAWRSEAEESKSKENGSLCCCEYVAEDSRERSHILGCCCNCVDFDLVCTRLVTCRAIDRRNIDGMLIAFQDRLRLPWRGGAKRISPAAVAPAFIVPLMLGLATLNSKTAIVLMLTLVGFTIWGMELAKRTATRTNFFLSWLVFSVFYMIIIFEFQVPLLELAPEENYALMFFSCAALYCLYSAKALSPLNLVSAQYGTTPKDELPGIAEASSGEEQAEAQTTLQMESVLSLDDDEVGDMDTAERSGLMHGQPNICEICRKVTPRRAYHCPVCGTCVKRRDHHSYWLNCCIGERNYVWYIVGLALSEIALLLGANLTLTSICHPFMVVRPLGYPVLLPDDCSEVFEGFDLGISFVVACYALLISSYIAFILARQAYLWWKGSTLHEYKRTSNAAGRNRIWSNWRAILK

Target ORF information:

RefSeq Version	NM_134897.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster beta4GalNAcTB pilot (GABPI), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134897.5

ORF Insert Sequence:

ATGTTCTTGA TGACAGCCGG CACCTCCAAT TTTCTTTCTG CAGCCTGGAG ATCAGAGGCT 
GAGGAGTCCA AGAGTAAGGA GAACGGGAGT CTCTGCTGCT GCGAGTACGT GGCTGAGGAC 
AGTCGAGAGC GATCCCACAT CCTAGGATGC TGTTGTAACT GCGTGGACTT CGACTTAGTT 
TGCACAAGAC TTGTCACCTG CCGAGCCATT GATCGAAGGA ACATCGACGG CATGCTCATC 
GCCTTCCAGG ATAGACTGCG CTTGCCATGG CGCGGAGGAG CCAAGCGAAT CTCGCCCGCA 
GCCGTTGCTC CCGCCTTCAT TGTCCCCCTC ATGCTGGGAT TGGCCACTCT GAACTCAAAG 
ACCGCCATAG TGCTGATGCT CACGCTGGTG GGCTTCACCA TCTGGGGCAT GGAGCTGGCT 
AAGCGGACCG CCACGCGGAC AAACTTCTTT TTGAGCTGGC TGGTCTTCTC GGTGTTCTAC 
ATGATCATCA TATTTGAGTT TCAGGTTCCT CTGCTGGAGC TGGCTCCGGA GGAGAACTAC 
GCGTTAATGT TCTTCTCCTG TGCCGCTTTG TACTGCCTCT ATTCCGCCAA GGCACTGTCG 
CCCTTGAATC TTGTTTCTGC TCAATACGGA ACCACGCCCA AGGATGAGCT GCCTGGGATC 
GCCGAGGCTT CGAGTGGCGA GGAGCAGGCA GAGGCCCAGA CCACACTCCA GATGGAGAGT 
GTGCTGAGTT TGGATGATGA CGAGGTGGGG GATATGGACA CTGCTGAGCG ATCTGGCTTG 
ATGCACGGTC AGCCCAATAT CTGCGAGATC TGCCGGAAGG TTACTCCAAG GCGGGCATAC 
CACTGTCCCG TCTGCGGAAC CTGCGTGAAG AGGCGCGATC ATCACAGCTA CTGGCTAAAC 
TGCTGCATTG GCGAGCGGAA CTACGTGTGG TACATTGTTG GACTGGCCCT GTCGGAGATC 
GCTCTGCTGC TCGGCGCAAA CTTGACCCTC ACTTCCATTT GCCATCCGTT TATGGTGGTG 
CGACCGCTTG GTTATCCAGT TCTTTTGCCC GACGACTGCA GTGAGGTGTT CGAAGGCTTC 
GACCTCGGAA TCTCCTTTGT GGTGGCTTGC TACGCTCTGC TCATTTCTTC CTACATAGCC 
TTTATACTCG CTAGACAGGC TTACTTGTGG TGGAAGGGAT CCACCTTGCA CGAATACAAG 
CGCACCTCCA ACGCCGCAGG ACGCAACAGA ATCTGGAGCA ACTGGCGAGC CATCTTAAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GABPI ( NM_164522.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_722869.1 Drosophila melanogaster beta4GalNAcTB pilot (GABPI), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa13592
Clone ID Related Accession (Same CDS sequence)	NM_134897.5 , NM_164522.3
Accession Version	NM_164522.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1263bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	beta4GalNAcTB pilot, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164522.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCTTGA TGACAGCCGG CACCTCCAAT TTTCTTTCTG CAGCCTGGAG ATCAGAGGCT 
GAGGAGTCCA AGAGTAAGGA GAACGGGAGT CTCTGCTGCT GCGAGTACGT GGCTGAGGAC 
AGTCGAGAGC GATCCCACAT CCTAGGATGC TGTTGTAACT GCGTGGACTT CGACTTAGTT 
TGCACAAGAC TTGTCACCTG CCGAGCCATT GATCGAAGGA ACATCGACGG CATGCTCATC 
GCCTTCCAGG ATAGACTGCG CTTGCCATGG CGCGGAGGAG CCAAGCGAAT CTCGCCCGCA 
GCCGTTGCTC CCGCCTTCAT TGTCCCCCTC ATGCTGGGAT TGGCCACTCT GAACTCAAAG 
ACCGCCATAG TGCTGATGCT CACGCTGGTG GGCTTCACCA TCTGGGGCAT GGAGCTGGCT 
AAGCGGACCG CCACGCGGAC AAACTTCTTT TTGAGCTGGC TGGTCTTCTC GGTGTTCTAC 
ATGATCATCA TATTTGAGTT TCAGGTTCCT CTGCTGGAGC TGGCTCCGGA GGAGAACTAC 
GCGTTAATGT TCTTCTCCTG TGCCGCTTTG TACTGCCTCT ATTCCGCCAA GGCACTGTCG 
CCCTTGAATC TTGTTTCTGC TCAATACGGA ACCACGCCCA AGGATGAGCT GCCTGGGATC 
GCCGAGGCTT CGAGTGGCGA GGAGCAGGCA GAGGCCCAGA CCACACTCCA GATGGAGAGT 
GTGCTGAGTT TGGATGATGA CGAGGTGGGG GATATGGACA CTGCTGAGCG ATCTGGCTTG 
ATGCACGGTC AGCCCAATAT CTGCGAGATC TGCCGGAAGG TTACTCCAAG GCGGGCATAC 
CACTGTCCCG TCTGCGGAAC CTGCGTGAAG AGGCGCGATC ATCACAGCTA CTGGCTAAAC 
TGCTGCATTG GCGAGCGGAA CTACGTGTGG TACATTGTTG GACTGGCCCT GTCGGAGATC 
GCTCTGCTGC TCGGCGCAAA CTTGACCCTC ACTTCCATTT GCCATCCGTT TATGGTGGTG 
CGACCGCTTG GTTATCCAGT TCTTTTGCCC GACGACTGCA GTGAGGTGTT CGAAGGCTTC 
GACCTCGGAA TCTCCTTTGT GGTGGCTTGC TACGCTCTGC TCATTTCTTC CTACATAGCC 
TTTATACTCG CTAGACAGGC TTACTTGTGG TGGAAGGGAT CCACCTTGCA CGAATACAAG 
CGCACCTCCA ACGCCGCAGG ACGCAACAGA ATCTGGAGCA ACTGGCGAGC CATCTTAAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_722869.1
CDS	222..1484
Translation	MFLMTAGTSNFLSAAWRSEAEESKSKENGSLCCCEYVAEDSRERSHILGCCCNCVDFDLVCTRLVTCRAIDRRNIDGMLIAFQDRLRLPWRGGAKRISPAAVAPAFIVPLMLGLATLNSKTAIVLMLTLVGFTIWGMELAKRTATRTNFFLSWLVFSVFYMIIIFEFQVPLLELAPEENYALMFFSCAALYCLYSAKALSPLNLVSAQYGTTPKDELPGIAEASSGEEQAEAQTTLQMESVLSLDDDEVGDMDTAERSGLMHGQPNICEICRKVTPRRAYHCPVCGTCVKRRDHHSYWLNCCIGERNYVWYIVGLALSEIALLLGANLTLTSICHPFMVVRPLGYPVLLPDDCSEVFEGFDLGISFVVACYALLISSYIAFILARQAYLWWKGSTLHEYKRTSNAAGRNRIWSNWRAILK

Target ORF information:

RefSeq Version	NM_164522.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster beta4GalNAcTB pilot (GABPI), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164522.3

ORF Insert Sequence:

ATGTTCTTGA TGACAGCCGG CACCTCCAAT TTTCTTTCTG CAGCCTGGAG ATCAGAGGCT 
GAGGAGTCCA AGAGTAAGGA GAACGGGAGT CTCTGCTGCT GCGAGTACGT GGCTGAGGAC 
AGTCGAGAGC GATCCCACAT CCTAGGATGC TGTTGTAACT GCGTGGACTT CGACTTAGTT 
TGCACAAGAC TTGTCACCTG CCGAGCCATT GATCGAAGGA ACATCGACGG CATGCTCATC 
GCCTTCCAGG ATAGACTGCG CTTGCCATGG CGCGGAGGAG CCAAGCGAAT CTCGCCCGCA 
GCCGTTGCTC CCGCCTTCAT TGTCCCCCTC ATGCTGGGAT TGGCCACTCT GAACTCAAAG 
ACCGCCATAG TGCTGATGCT CACGCTGGTG GGCTTCACCA TCTGGGGCAT GGAGCTGGCT 
AAGCGGACCG CCACGCGGAC AAACTTCTTT TTGAGCTGGC TGGTCTTCTC GGTGTTCTAC 
ATGATCATCA TATTTGAGTT TCAGGTTCCT CTGCTGGAGC TGGCTCCGGA GGAGAACTAC 
GCGTTAATGT TCTTCTCCTG TGCCGCTTTG TACTGCCTCT ATTCCGCCAA GGCACTGTCG 
CCCTTGAATC TTGTTTCTGC TCAATACGGA ACCACGCCCA AGGATGAGCT GCCTGGGATC 
GCCGAGGCTT CGAGTGGCGA GGAGCAGGCA GAGGCCCAGA CCACACTCCA GATGGAGAGT 
GTGCTGAGTT TGGATGATGA CGAGGTGGGG GATATGGACA CTGCTGAGCG ATCTGGCTTG 
ATGCACGGTC AGCCCAATAT CTGCGAGATC TGCCGGAAGG TTACTCCAAG GCGGGCATAC 
CACTGTCCCG TCTGCGGAAC CTGCGTGAAG AGGCGCGATC ATCACAGCTA CTGGCTAAAC 
TGCTGCATTG GCGAGCGGAA CTACGTGTGG TACATTGTTG GACTGGCCCT GTCGGAGATC 
GCTCTGCTGC TCGGCGCAAA CTTGACCCTC ACTTCCATTT GCCATCCGTT TATGGTGGTG 
CGACCGCTTG GTTATCCAGT TCTTTTGCCC GACGACTGCA GTGAGGTGTT CGAAGGCTTC 
GACCTCGGAA TCTCCTTTGT GGTGGCTTGC TACGCTCTGC TCATTTCTTC CTACATAGCC 
TTTATACTCG CTAGACAGGC TTACTTGTGG TGGAAGGGAT CCACCTTGCA CGAATACAAG 
CGCACCTCCA ACGCCGCAGG ACGCAACAGA ATCTGGAGCA ACTGGCGAGC CATCTTAAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GABPI ( NM_001298645.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285574.1 Drosophila melanogaster beta4GalNAcTB pilot (GABPI), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa21582
Clone ID Related Accession (Same CDS sequence)	NM_001298645.1 , NM_001298644.1
Accession Version	NM_001298645.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1032bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	beta4GalNAcTB pilot, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTCATCG CCTTCCAGGA TAGACTGCGC TTGCCATGGC GCGGAGGAGC CAAGCGAATC 
TCGCCCGCAG CCGTTGCTCC CGCCTTCATT GTCCCCCTCA TGCTGGGATT GGCCACTCTG 
AACTCAAAGA CCGCCATAGT GCTGATGCTC ACGCTGGTGG GCTTCACCAT CTGGGGCATG 
GAGCTGGCTA AGCGGACCGC CACGCGGACA AACTTCTTTT TGAGCTGGCT GGTCTTCTCG 
GTGTTCTACA TGATCATCAT ATTTGAGTTT CAGGTTCCTC TGCTGGAGCT GGCTCCGGAG 
GAGAACTACG CGTTAATGTT CTTCTCCTGT GCCGCTTTGT ACTGCCTCTA TTCCGCCAAG 
GCACTGTCGC CCTTGAATCT TGTTTCTGCT CAATACGGAA CCACGCCCAA GGATGAGCTG 
CCTGGGATCG CCGAGGCTTC GAGTGGCGAG GAGCAGGCAG AGGCCCAGAC CACACTCCAG 
ATGGAGAGTG TGCTGAGTTT GGATGATGAC GAGGTGGGGG ATATGGACAC TGCTGAGCGA 
TCTGGCTTGA TGCACGGTCA GCCCAATATC TGCGAGATCT GCCGGAAGGT TACTCCAAGG 
CGGGCATACC ACTGTCCCGT CTGCGGAACC TGCGTGAAGA GGCGCGATCA TCACAGCTAC 
TGGCTAAACT GCTGCATTGG CGAGCGGAAC TACGTGTGGT ACATTGTTGG ACTGGCCCTG 
TCGGAGATCG CTCTGCTGCT CGGCGCAAAC TTGACCCTCA CTTCCATTTG CCATCCGTTT 
ATGGTGGTGC GACCGCTTGG TTATCCAGTT CTTTTGCCCG ACGACTGCAG TGAGGTGTTC 
GAAGGCTTCG ACCTCGGAAT CTCCTTTGTG GTGGCTTGCT ACGCTCTGCT CATTTCTTCC 
TACATAGCCT TTATACTCGC TAGACAGGCT TACTTGTGGT GGAAGGGATC CACCTTGCAC 
GAATACAAGC GCACCTCCAA CGCCGCAGGA CGCAACAGAA TCTGGAGCAA CTGGCGAGCC 
ATCTTAAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285574.1
CDS	253..1284
Translation	MLIAFQDRLRLPWRGGAKRISPAAVAPAFIVPLMLGLATLNSKTAIVLMLTLVGFTIWGMELAKRTATRTNFFLSWLVFSVFYMIIIFEFQVPLLELAPEENYALMFFSCAALYCLYSAKALSPLNLVSAQYGTTPKDELPGIAEASSGEEQAEAQTTLQMESVLSLDDDEVGDMDTAERSGLMHGQPNICEICRKVTPRRAYHCPVCGTCVKRRDHHSYWLNCCIGERNYVWYIVGLALSEIALLLGANLTLTSICHPFMVVRPLGYPVLLPDDCSEVFEGFDLGISFVVACYALLISSYIAFILARQAYLWWKGSTLHEYKRTSNAAGRNRIWSNWRAILK

Target ORF information:

RefSeq Version	NM_001298645.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster beta4GalNAcTB pilot (GABPI), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298645.1

ORF Insert Sequence:

ATGCTCATCG CCTTCCAGGA TAGACTGCGC TTGCCATGGC GCGGAGGAGC CAAGCGAATC 
TCGCCCGCAG CCGTTGCTCC CGCCTTCATT GTCCCCCTCA TGCTGGGATT GGCCACTCTG 
AACTCAAAGA CCGCCATAGT GCTGATGCTC ACGCTGGTGG GCTTCACCAT CTGGGGCATG 
GAGCTGGCTA AGCGGACCGC CACGCGGACA AACTTCTTTT TGAGCTGGCT GGTCTTCTCG 
GTGTTCTACA TGATCATCAT ATTTGAGTTT CAGGTTCCTC TGCTGGAGCT GGCTCCGGAG 
GAGAACTACG CGTTAATGTT CTTCTCCTGT GCCGCTTTGT ACTGCCTCTA TTCCGCCAAG 
GCACTGTCGC CCTTGAATCT TGTTTCTGCT CAATACGGAA CCACGCCCAA GGATGAGCTG 
CCTGGGATCG CCGAGGCTTC GAGTGGCGAG GAGCAGGCAG AGGCCCAGAC CACACTCCAG 
ATGGAGAGTG TGCTGAGTTT GGATGATGAC GAGGTGGGGG ATATGGACAC TGCTGAGCGA 
TCTGGCTTGA TGCACGGTCA GCCCAATATC TGCGAGATCT GCCGGAAGGT TACTCCAAGG 
CGGGCATACC ACTGTCCCGT CTGCGGAACC TGCGTGAAGA GGCGCGATCA TCACAGCTAC 
TGGCTAAACT GCTGCATTGG CGAGCGGAAC TACGTGTGGT ACATTGTTGG ACTGGCCCTG 
TCGGAGATCG CTCTGCTGCT CGGCGCAAAC TTGACCCTCA CTTCCATTTG CCATCCGTTT 
ATGGTGGTGC GACCGCTTGG TTATCCAGTT CTTTTGCCCG ACGACTGCAG TGAGGTGTTC 
GAAGGCTTCG ACCTCGGAAT CTCCTTTGTG GTGGCTTGCT ACGCTCTGCT CATTTCTTCC 
TACATAGCCT TTATACTCGC TAGACAGGCT TACTTGTGGT GGAAGGGATC CACCTTGCAC 
GAATACAAGC GCACCTCCAA CGCCGCAGGA CGCAACAGAA TCTGGAGCAA CTGGCGAGCC 
ATCTTAAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GABPI ( NM_001298644.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285573.1 Drosophila melanogaster beta4GalNAcTB pilot (GABPI), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa21582
Clone ID Related Accession (Same CDS sequence)	NM_001298645.1 , NM_001298644.1
Accession Version	NM_001298644.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1032bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	beta4GalNAcTB pilot, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTCATCG CCTTCCAGGA TAGACTGCGC TTGCCATGGC GCGGAGGAGC CAAGCGAATC 
TCGCCCGCAG CCGTTGCTCC CGCCTTCATT GTCCCCCTCA TGCTGGGATT GGCCACTCTG 
AACTCAAAGA CCGCCATAGT GCTGATGCTC ACGCTGGTGG GCTTCACCAT CTGGGGCATG 
GAGCTGGCTA AGCGGACCGC CACGCGGACA AACTTCTTTT TGAGCTGGCT GGTCTTCTCG 
GTGTTCTACA TGATCATCAT ATTTGAGTTT CAGGTTCCTC TGCTGGAGCT GGCTCCGGAG 
GAGAACTACG CGTTAATGTT CTTCTCCTGT GCCGCTTTGT ACTGCCTCTA TTCCGCCAAG 
GCACTGTCGC CCTTGAATCT TGTTTCTGCT CAATACGGAA CCACGCCCAA GGATGAGCTG 
CCTGGGATCG CCGAGGCTTC GAGTGGCGAG GAGCAGGCAG AGGCCCAGAC CACACTCCAG 
ATGGAGAGTG TGCTGAGTTT GGATGATGAC GAGGTGGGGG ATATGGACAC TGCTGAGCGA 
TCTGGCTTGA TGCACGGTCA GCCCAATATC TGCGAGATCT GCCGGAAGGT TACTCCAAGG 
CGGGCATACC ACTGTCCCGT CTGCGGAACC TGCGTGAAGA GGCGCGATCA TCACAGCTAC 
TGGCTAAACT GCTGCATTGG CGAGCGGAAC TACGTGTGGT ACATTGTTGG ACTGGCCCTG 
TCGGAGATCG CTCTGCTGCT CGGCGCAAAC TTGACCCTCA CTTCCATTTG CCATCCGTTT 
ATGGTGGTGC GACCGCTTGG TTATCCAGTT CTTTTGCCCG ACGACTGCAG TGAGGTGTTC 
GAAGGCTTCG ACCTCGGAAT CTCCTTTGTG GTGGCTTGCT ACGCTCTGCT CATTTCTTCC 
TACATAGCCT TTATACTCGC TAGACAGGCT TACTTGTGGT GGAAGGGATC CACCTTGCAC 
GAATACAAGC GCACCTCCAA CGCCGCAGGA CGCAACAGAA TCTGGAGCAA CTGGCGAGCC 
ATCTTAAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285573.1
CDS	505..1536
Translation	MLIAFQDRLRLPWRGGAKRISPAAVAPAFIVPLMLGLATLNSKTAIVLMLTLVGFTIWGMELAKRTATRTNFFLSWLVFSVFYMIIIFEFQVPLLELAPEENYALMFFSCAALYCLYSAKALSPLNLVSAQYGTTPKDELPGIAEASSGEEQAEAQTTLQMESVLSLDDDEVGDMDTAERSGLMHGQPNICEICRKVTPRRAYHCPVCGTCVKRRDHHSYWLNCCIGERNYVWYIVGLALSEIALLLGANLTLTSICHPFMVVRPLGYPVLLPDDCSEVFEGFDLGISFVVACYALLISSYIAFILARQAYLWWKGSTLHEYKRTSNAAGRNRIWSNWRAILK

Target ORF information:

RefSeq Version	NM_001298644.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster beta4GalNAcTB pilot (GABPI), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298644.1

ORF Insert Sequence:

ATGCTCATCG CCTTCCAGGA TAGACTGCGC TTGCCATGGC GCGGAGGAGC CAAGCGAATC 
TCGCCCGCAG CCGTTGCTCC CGCCTTCATT GTCCCCCTCA TGCTGGGATT GGCCACTCTG 
AACTCAAAGA CCGCCATAGT GCTGATGCTC ACGCTGGTGG GCTTCACCAT CTGGGGCATG 
GAGCTGGCTA AGCGGACCGC CACGCGGACA AACTTCTTTT TGAGCTGGCT GGTCTTCTCG 
GTGTTCTACA TGATCATCAT ATTTGAGTTT CAGGTTCCTC TGCTGGAGCT GGCTCCGGAG 
GAGAACTACG CGTTAATGTT CTTCTCCTGT GCCGCTTTGT ACTGCCTCTA TTCCGCCAAG 
GCACTGTCGC CCTTGAATCT TGTTTCTGCT CAATACGGAA CCACGCCCAA GGATGAGCTG 
CCTGGGATCG CCGAGGCTTC GAGTGGCGAG GAGCAGGCAG AGGCCCAGAC CACACTCCAG 
ATGGAGAGTG TGCTGAGTTT GGATGATGAC GAGGTGGGGG ATATGGACAC TGCTGAGCGA 
TCTGGCTTGA TGCACGGTCA GCCCAATATC TGCGAGATCT GCCGGAAGGT TACTCCAAGG 
CGGGCATACC ACTGTCCCGT CTGCGGAACC TGCGTGAAGA GGCGCGATCA TCACAGCTAC 
TGGCTAAACT GCTGCATTGG CGAGCGGAAC TACGTGTGGT ACATTGTTGG ACTGGCCCTG 
TCGGAGATCG CTCTGCTGCT CGGCGCAAAC TTGACCCTCA CTTCCATTTG CCATCCGTTT 
ATGGTGGTGC GACCGCTTGG TTATCCAGTT CTTTTGCCCG ACGACTGCAG TGAGGTGTTC 
GAAGGCTTCG ACCTCGGAAT CTCCTTTGTG GTGGCTTGCT ACGCTCTGCT CATTTCTTCC 
TACATAGCCT TTATACTCGC TAGACAGGCT TACTTGTGGT GGAAGGGATC CACCTTGCAC 
GAATACAAGC GCACCTCCAA CGCCGCAGGA CGCAACAGAA TCTGGAGCAA CTGGCGAGCC 
ATCTTAAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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GABPI cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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