Sf3b2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sf3b2
Entrez Gene ID	33514
Full Name	CG3605 gene product from transcript CG3605-RB
Synonyms	Dmel\CG3605
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 23C4-23C4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001273050.1	NP_001259979.1	splicing factor 3b subunit 2, isoform B
NM_134895.4	NP_608739.1	splicing factor 3b subunit 2, isoform A

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	CELE_W03F9.10	NP_503141.1
Oryza sativa (rice)	Os02g0827300	NP_001048592.1
Rattus norvegicus (Norway rat)	Sf3b2	NP_001099796.2
Homo sapiens (human)	SF3B2	NP_006833.2
	SF3B2	XP_002799525.1
Canis lupus familiaris (dog)	SF3B2	XP_533224.2
Xenopus tropicalis (tropical clawed frog)	sf3b2	XP_002935399.1
Bos taurus (cattle)	SF3B2	NP_001096741.1
Mus musculus (house mouse)	Sf3b2	NP_084385.2
Drosophila melanogaster (fruit fly)	CG3605	NP_608739.1
Arabidopsis thaliana (thale cress)	AT4G21660	NP_001190789.1
Pan troglodytes (chimpanzee)	SF3B2	XP_508570.2
Danio rerio (zebrafish)	sf3b2	NP_001098747.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006240	XP_316308.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Sf3b2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sf3b2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa13590	NM_001273050.1 Latest version!	Drosophila melanogaster splicing factor 3b subunit 2, transcript variant B (Sf3b2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa13590	NM_134895.4 Latest version!	Drosophila melanogaster splicing factor 3b subunit 2, transcript variant A (Sf3b2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sf3b2 ( NM_001273050.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259979.1 Drosophila melanogaster splicing factor 3b subunit 2, transcript variant B (Sf3b2), mRNA.

Price & Availability↑

CloneID	OFa13590
Clone ID Related Accession (Same CDS sequence)	NM_001273050.1 , NM_134895.4
Accession Version	NM_001273050.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	splicing factor 3b subunit 2, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGACC AGTTGAACAA CGAGCCCGGC CAACCCACGC CGCTCATGTC CATTCGCTTC 
GACCAGCAGC AGCACGAAAG TGCGGCGGCC AATGGAAATG AGAACGGCAA TGGTAATGCC 
AATGGCGACG GCGATGCCTC CGAAGATGCT GTTGATGCCG AAAAAACGCC CGGAGAACTG 
GTCCTGCCCA AGGCCTTGGA GGACGTTCTG GCTTTGAAGG ATCAAAGGGC AGCCGAGTTC 
ACGGTGGACG CCGATTCGCA GGGTGGCAGT GGGGGTGCTA CCCAGGACGG CGACGAAGCG 
GATGTGGGCG AGGACAGCGA GGAGGATGCG GATAACCAGG TGAACGGGAG TGCCAGCCAA 
AAGACCGGCA AGCAGAGCAA GGCGGAGCGT AACAAAAAGA AGAAGAAGCG AAAGAAGCAG 
AACAGGAAGA TACGCCACCA GATTGAGTTC GCACGGCAGC AGCAGTTGGC CCAAGCGGAG 
GAGAACGAAC CGGAGGCCAA GGACTCGGAG ACAAAAGCCC ATGGCAGCGA GGACGAACAG 
GTTGCCGAAA AGGAAAAGAA GGACAAACGC AAAGAGAGTC GCAGCAAGCG CAGGAAGGAT 
CGTAGTGATG AGAAAGATAC GGACAAAAAG GCTGGCGAGG CCAATGATGA AAATGTGACC 
ATTGAATATG TGCCCGAAAA GATAACCATT GCCGATTTGG CGCCAATGTA CCGCCAGTTT 
TATCGTGTGT TCGAAATCTT CAAGCTGGAA AACAAACCAA AGCCTGTGGA GAAGGACAAA 
TCTTCGCACG ATTCTGAGTC CCATCCCAGG GACAAAAAGG CTACAGACAA ACAGCTCGAG 
GACGAGGACG ATGACGGCGA TGATGACGAA GAGCGCAAGG AAGACAAGGA GAAGCTGTCC 
AAGCGGAAAC TTAAGAAACT GACCCGTTTG AGTGTGGCAG AACTGAAACA ACTGGTCTCT 
CGACCTGACG TAGTGGAGAT GCACGATGTC ACAGCGAGAG ATCCCAAACT ACTGGTTCAA 
CTGAAAGCCT ATAGGAACAC AGTACAAGTG CCGCGGCATT GGTGCTTCAA GCGAAAATAT 
CTGCAGGGCA AAAGGGGTAT TGAGAAGCCT CCGTTCGATC TCCCCGCCTT CATCAAAAAG 
ACGGGCATCA TGGAGATGCG CGAATCGTTG CAGGAACGAG AGGACGCCAA GACACTCAAG 
GCCAAGATGC GTGAACGCGT CCGTCCCAAG ATGGGCAAAA TTGACATTGA CTACCAGAAA 
CTTCACGATG CCTTCTTCAA GTGGCAGACC AAGCCCCGCA TGACCATTCA TGGCGACCTC 
TACTACGAGG GCAAGGAGTT TGAGACACGC CTTAAGGAAA AGAAGCCCGG AGATCTCTCC 
GAGGAGTTGC GCATTGCTCT GGGAATGCCC GTGGGACCCA ACTCACACAA GATTCCGCCA 
CCATGGCTCA TTGCTCAACA GCGCTACGGG CCGCCGCCCT CGTATCCGAA TCTTAAAATT 
CCTGGCCTGA ATGCTCCGAT CCCAGACGGA ACCTCGTTCG GTTATCATGC CGGAGGTTGG 
GGCAAGCCGC CAGTGGATGA GAACGGAAAA CCGCTGTACG GTGACGTATT TGGAACCAAC 
ATATTGGACC TTGATAATGG CGTAGACGAA GCTGACATTG AACGTAACCA ATGGGGCGAA 
CTGGAATCGG AGTCAGAAGA ATCATCCGAG GAGGAAGAGG AAGATGGCGA GGACTTGGGC 
GATCAGCAGG ATGAGACTGG CCTGGTAACG CCAGTAGAGG GACTAGTAAC ACCCTCTGGA 
CTCACCAGTG TTCCAGCTGG CATGGAAACC CCCGAGAACA TTGAGCTTCG CAAGAAGAAA 
ATTGAAGCCG AGATGGAGGA CAACGAAACT CCTGTTCTGT ATCAAGTGCT GCCTGAGAAG 
CGCACCGACC GCATAGGGGC TTCCATGATG GGATCAACCC ATGTCTACGA CGTCAGTGGC 
AGTGGGGCTA ACAAACAGCC CCCAGTTCGA TCAACCACAG ATCGCGAGGG CATTGTGGAA 
CTGGCTCTCG ATCCTAGCGA ATTGGACTTG GACAATGACG CAATGGCCCA GCGTTATGAG 
CAACAAATGC GTGAGCAGCA GAATCACCTG CAAAAGGAGG ATCTGTCAGA CATGTTGGCC 
GAACATGTGG CGCGGCAGAA ATCGAAGCGC AAGCGACAGC AAACGGATCC GGCCAAGACC 
ACAAAGAAGT ACAAGGAGTT CAAGTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259979.1
CDS	82..2331
Translation	MADQLNNEPGQPTPLMSIRFDQQQHESAAANGNENGNGNANGDGDASEDAVDAEKTPGELVLPKALEDVLALKDQRAAEFTVDADSQGGSGGATQDGDEADVGEDSEEDADNQVNGSASQKTGKQSKAERNKKKKKRKKQNRKIRHQIEFARQQQLAQAEENEPEAKDSETKAHGSEDEQVAEKEKKDKRKESRSKRRKDRSDEKDTDKKAGEANDENVTIEYVPEKITIADLAPMYRQFYRVFEIFKLENKPKPVEKDKSSHDSESHPRDKKATDKQLEDEDDDGDDDEERKEDKEKLSKRKLKKLTRLSVAELKQLVSRPDVVEMHDVTARDPKLLVQLKAYRNTVQVPRHWCFKRKYLQGKRGIEKPPFDLPAFIKKTGIMEMRESLQEREDAKTLKAKMRERVRPKMGKIDIDYQKLHDAFFKWQTKPRMTIHGDLYYEGKEFETRLKEKKPGDLSEELRIALGMPVGPNSHKIPPPWLIAQQRYGPPPSYPNLKIPGLNAPIPDGTSFGYHAGGWGKPPVDENGKPLYGDVFGTNILDLDNGVDEADIERNQWGELESESEESSEEEEEDGEDLGDQQDETGLVTPVEGLVTPSGLTSVPAGMETPENIELRKKKIEAEMEDNETPVLYQVLPEKRTDRIGASMMGSTHVYDVSGSGANKQPPVRSTTDREGIVELALDPSELDLDNDAMAQRYEQQMREQQNHLQKEDLSDMLAEHVARQKSKRKRQQTDPAKTTKKYKEFKF

Target ORF information:

RefSeq Version	NM_001273050.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster splicing factor 3b subunit 2, transcript variant B (Sf3b2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273050.1

ORF Insert Sequence:

ATGGCGGACC AGTTGAACAA CGAGCCCGGC CAACCCACGC CGCTCATGTC CATTCGCTTC 
GACCAGCAGC AGCACGAAAG TGCGGCGGCC AATGGAAATG AGAACGGCAA TGGTAATGCC 
AATGGCGACG GCGATGCCTC CGAAGATGCT GTTGATGCCG AAAAAACGCC CGGAGAACTG 
GTCCTGCCCA AGGCCTTGGA GGACGTTCTG GCTTTGAAGG ATCAAAGGGC AGCCGAGTTC 
ACGGTGGACG CCGATTCGCA GGGTGGCAGT GGGGGTGCTA CCCAGGACGG CGACGAAGCG 
GATGTGGGCG AGGACAGCGA GGAGGATGCG GATAACCAGG TGAACGGGAG TGCCAGCCAA 
AAGACCGGCA AGCAGAGCAA GGCGGAGCGT AACAAAAAGA AGAAGAAGCG AAAGAAGCAG 
AACAGGAAGA TACGCCACCA GATTGAGTTC GCACGGCAGC AGCAGTTGGC CCAAGCGGAG 
GAGAACGAAC CGGAGGCCAA GGACTCGGAG ACAAAAGCCC ATGGCAGCGA GGACGAACAG 
GTTGCCGAAA AGGAAAAGAA GGACAAACGC AAAGAGAGTC GCAGCAAGCG CAGGAAGGAT 
CGTAGTGATG AGAAAGATAC GGACAAAAAG GCTGGCGAGG CCAATGATGA AAATGTGACC 
ATTGAATATG TGCCCGAAAA GATAACCATT GCCGATTTGG CGCCAATGTA CCGCCAGTTT 
TATCGTGTGT TCGAAATCTT CAAGCTGGAA AACAAACCAA AGCCTGTGGA GAAGGACAAA 
TCTTCGCACG ATTCTGAGTC CCATCCCAGG GACAAAAAGG CTACAGACAA ACAGCTCGAG 
GACGAGGACG ATGACGGCGA TGATGACGAA GAGCGCAAGG AAGACAAGGA GAAGCTGTCC 
AAGCGGAAAC TTAAGAAACT GACCCGTTTG AGTGTGGCAG AACTGAAACA ACTGGTCTCT 
CGACCTGACG TAGTGGAGAT GCACGATGTC ACAGCGAGAG ATCCCAAACT ACTGGTTCAA 
CTGAAAGCCT ATAGGAACAC AGTACAAGTG CCGCGGCATT GGTGCTTCAA GCGAAAATAT 
CTGCAGGGCA AAAGGGGTAT TGAGAAGCCT CCGTTCGATC TCCCCGCCTT CATCAAAAAG 
ACGGGCATCA TGGAGATGCG CGAATCGTTG CAGGAACGAG AGGACGCCAA GACACTCAAG 
GCCAAGATGC GTGAACGCGT CCGTCCCAAG ATGGGCAAAA TTGACATTGA CTACCAGAAA 
CTTCACGATG CCTTCTTCAA GTGGCAGACC AAGCCCCGCA TGACCATTCA TGGCGACCTC 
TACTACGAGG GCAAGGAGTT TGAGACACGC CTTAAGGAAA AGAAGCCCGG AGATCTCTCC 
GAGGAGTTGC GCATTGCTCT GGGAATGCCC GTGGGACCCA ACTCACACAA GATTCCGCCA 
CCATGGCTCA TTGCTCAACA GCGCTACGGG CCGCCGCCCT CGTATCCGAA TCTTAAAATT 
CCTGGCCTGA ATGCTCCGAT CCCAGACGGA ACCTCGTTCG GTTATCATGC CGGAGGTTGG 
GGCAAGCCGC CAGTGGATGA GAACGGAAAA CCGCTGTACG GTGACGTATT TGGAACCAAC 
ATATTGGACC TTGATAATGG CGTAGACGAA GCTGACATTG AACGTAACCA ATGGGGCGAA 
CTGGAATCGG AGTCAGAAGA ATCATCCGAG GAGGAAGAGG AAGATGGCGA GGACTTGGGC 
GATCAGCAGG ATGAGACTGG CCTGGTAACG CCAGTAGAGG GACTAGTAAC ACCCTCTGGA 
CTCACCAGTG TTCCAGCTGG CATGGAAACC CCCGAGAACA TTGAGCTTCG CAAGAAGAAA 
ATTGAAGCCG AGATGGAGGA CAACGAAACT CCTGTTCTGT ATCAAGTGCT GCCTGAGAAG 
CGCACCGACC GCATAGGGGC TTCCATGATG GGATCAACCC ATGTCTACGA CGTCAGTGGC 
AGTGGGGCTA ACAAACAGCC CCCAGTTCGA TCAACCACAG ATCGCGAGGG CATTGTGGAA 
CTGGCTCTCG ATCCTAGCGA ATTGGACTTG GACAATGACG CAATGGCCCA GCGTTATGAG 
CAACAAATGC GTGAGCAGCA GAATCACCTG CAAAAGGAGG ATCTGTCAGA CATGTTGGCC 
GAACATGTGG CGCGGCAGAA ATCGAAGCGC AAGCGACAGC AAACGGATCC GGCCAAGACC 
ACAAAGAAGT ACAAGGAGTT CAAGTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sf3b2 ( NM_134895.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_608739.1 Drosophila melanogaster splicing factor 3b subunit 2, transcript variant A (Sf3b2), mRNA.

Price & Availability↑

CloneID	OFa13590
Clone ID Related Accession (Same CDS sequence)	NM_001273050.1 , NM_134895.4
Accession Version	NM_134895.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	splicing factor 3b subunit 2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_134895.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGACC AGTTGAACAA CGAGCCCGGC CAACCCACGC CGCTCATGTC CATTCGCTTC 
GACCAGCAGC AGCACGAAAG TGCGGCGGCC AATGGAAATG AGAACGGCAA TGGTAATGCC 
AATGGCGACG GCGATGCCTC CGAAGATGCT GTTGATGCCG AAAAAACGCC CGGAGAACTG 
GTCCTGCCCA AGGCCTTGGA GGACGTTCTG GCTTTGAAGG ATCAAAGGGC AGCCGAGTTC 
ACGGTGGACG CCGATTCGCA GGGTGGCAGT GGGGGTGCTA CCCAGGACGG CGACGAAGCG 
GATGTGGGCG AGGACAGCGA GGAGGATGCG GATAACCAGG TGAACGGGAG TGCCAGCCAA 
AAGACCGGCA AGCAGAGCAA GGCGGAGCGT AACAAAAAGA AGAAGAAGCG AAAGAAGCAG 
AACAGGAAGA TACGCCACCA GATTGAGTTC GCACGGCAGC AGCAGTTGGC CCAAGCGGAG 
GAGAACGAAC CGGAGGCCAA GGACTCGGAG ACAAAAGCCC ATGGCAGCGA GGACGAACAG 
GTTGCCGAAA AGGAAAAGAA GGACAAACGC AAAGAGAGTC GCAGCAAGCG CAGGAAGGAT 
CGTAGTGATG AGAAAGATAC GGACAAAAAG GCTGGCGAGG CCAATGATGA AAATGTGACC 
ATTGAATATG TGCCCGAAAA GATAACCATT GCCGATTTGG CGCCAATGTA CCGCCAGTTT 
TATCGTGTGT TCGAAATCTT CAAGCTGGAA AACAAACCAA AGCCTGTGGA GAAGGACAAA 
TCTTCGCACG ATTCTGAGTC CCATCCCAGG GACAAAAAGG CTACAGACAA ACAGCTCGAG 
GACGAGGACG ATGACGGCGA TGATGACGAA GAGCGCAAGG AAGACAAGGA GAAGCTGTCC 
AAGCGGAAAC TTAAGAAACT GACCCGTTTG AGTGTGGCAG AACTGAAACA ACTGGTCTCT 
CGACCTGACG TAGTGGAGAT GCACGATGTC ACAGCGAGAG ATCCCAAACT ACTGGTTCAA 
CTGAAAGCCT ATAGGAACAC AGTACAAGTG CCGCGGCATT GGTGCTTCAA GCGAAAATAT 
CTGCAGGGCA AAAGGGGTAT TGAGAAGCCT CCGTTCGATC TCCCCGCCTT CATCAAAAAG 
ACGGGCATCA TGGAGATGCG CGAATCGTTG CAGGAACGAG AGGACGCCAA GACACTCAAG 
GCCAAGATGC GTGAACGCGT CCGTCCCAAG ATGGGCAAAA TTGACATTGA CTACCAGAAA 
CTTCACGATG CCTTCTTCAA GTGGCAGACC AAGCCCCGCA TGACCATTCA TGGCGACCTC 
TACTACGAGG GCAAGGAGTT TGAGACACGC CTTAAGGAAA AGAAGCCCGG AGATCTCTCC 
GAGGAGTTGC GCATTGCTCT GGGAATGCCC GTGGGACCCA ACTCACACAA GATTCCGCCA 
CCATGGCTCA TTGCTCAACA GCGCTACGGG CCGCCGCCCT CGTATCCGAA TCTTAAAATT 
CCTGGCCTGA ATGCTCCGAT CCCAGACGGA ACCTCGTTCG GTTATCATGC CGGAGGTTGG 
GGCAAGCCGC CAGTGGATGA GAACGGAAAA CCGCTGTACG GTGACGTATT TGGAACCAAC 
ATATTGGACC TTGATAATGG CGTAGACGAA GCTGACATTG AACGTAACCA ATGGGGCGAA 
CTGGAATCGG AGTCAGAAGA ATCATCCGAG GAGGAAGAGG AAGATGGCGA GGACTTGGGC 
GATCAGCAGG ATGAGACTGG CCTGGTAACG CCAGTAGAGG GACTAGTAAC ACCCTCTGGA 
CTCACCAGTG TTCCAGCTGG CATGGAAACC CCCGAGAACA TTGAGCTTCG CAAGAAGAAA 
ATTGAAGCCG AGATGGAGGA CAACGAAACT CCTGTTCTGT ATCAAGTGCT GCCTGAGAAG 
CGCACCGACC GCATAGGGGC TTCCATGATG GGATCAACCC ATGTCTACGA CGTCAGTGGC 
AGTGGGGCTA ACAAACAGCC CCCAGTTCGA TCAACCACAG ATCGCGAGGG CATTGTGGAA 
CTGGCTCTCG ATCCTAGCGA ATTGGACTTG GACAATGACG CAATGGCCCA GCGTTATGAG 
CAACAAATGC GTGAGCAGCA GAATCACCTG CAAAAGGAGG ATCTGTCAGA CATGTTGGCC 
GAACATGTGG CGCGGCAGAA ATCGAAGCGC AAGCGACAGC AAACGGATCC GGCCAAGACC 
ACAAAGAAGT ACAAGGAGTT CAAGTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_608739.1
CDS	82..2331
Translation	MADQLNNEPGQPTPLMSIRFDQQQHESAAANGNENGNGNANGDGDASEDAVDAEKTPGELVLPKALEDVLALKDQRAAEFTVDADSQGGSGGATQDGDEADVGEDSEEDADNQVNGSASQKTGKQSKAERNKKKKKRKKQNRKIRHQIEFARQQQLAQAEENEPEAKDSETKAHGSEDEQVAEKEKKDKRKESRSKRRKDRSDEKDTDKKAGEANDENVTIEYVPEKITIADLAPMYRQFYRVFEIFKLENKPKPVEKDKSSHDSESHPRDKKATDKQLEDEDDDGDDDEERKEDKEKLSKRKLKKLTRLSVAELKQLVSRPDVVEMHDVTARDPKLLVQLKAYRNTVQVPRHWCFKRKYLQGKRGIEKPPFDLPAFIKKTGIMEMRESLQEREDAKTLKAKMRERVRPKMGKIDIDYQKLHDAFFKWQTKPRMTIHGDLYYEGKEFETRLKEKKPGDLSEELRIALGMPVGPNSHKIPPPWLIAQQRYGPPPSYPNLKIPGLNAPIPDGTSFGYHAGGWGKPPVDENGKPLYGDVFGTNILDLDNGVDEADIERNQWGELESESEESSEEEEEDGEDLGDQQDETGLVTPVEGLVTPSGLTSVPAGMETPENIELRKKKIEAEMEDNETPVLYQVLPEKRTDRIGASMMGSTHVYDVSGSGANKQPPVRSTTDREGIVELALDPSELDLDNDAMAQRYEQQMREQQNHLQKEDLSDMLAEHVARQKSKRKRQQTDPAKTTKKYKEFKF

Target ORF information:

RefSeq Version	NM_134895.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster splicing factor 3b subunit 2, transcript variant A (Sf3b2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134895.4

ORF Insert Sequence:

ATGGCGGACC AGTTGAACAA CGAGCCCGGC CAACCCACGC CGCTCATGTC CATTCGCTTC 
GACCAGCAGC AGCACGAAAG TGCGGCGGCC AATGGAAATG AGAACGGCAA TGGTAATGCC 
AATGGCGACG GCGATGCCTC CGAAGATGCT GTTGATGCCG AAAAAACGCC CGGAGAACTG 
GTCCTGCCCA AGGCCTTGGA GGACGTTCTG GCTTTGAAGG ATCAAAGGGC AGCCGAGTTC 
ACGGTGGACG CCGATTCGCA GGGTGGCAGT GGGGGTGCTA CCCAGGACGG CGACGAAGCG 
GATGTGGGCG AGGACAGCGA GGAGGATGCG GATAACCAGG TGAACGGGAG TGCCAGCCAA 
AAGACCGGCA AGCAGAGCAA GGCGGAGCGT AACAAAAAGA AGAAGAAGCG AAAGAAGCAG 
AACAGGAAGA TACGCCACCA GATTGAGTTC GCACGGCAGC AGCAGTTGGC CCAAGCGGAG 
GAGAACGAAC CGGAGGCCAA GGACTCGGAG ACAAAAGCCC ATGGCAGCGA GGACGAACAG 
GTTGCCGAAA AGGAAAAGAA GGACAAACGC AAAGAGAGTC GCAGCAAGCG CAGGAAGGAT 
CGTAGTGATG AGAAAGATAC GGACAAAAAG GCTGGCGAGG CCAATGATGA AAATGTGACC 
ATTGAATATG TGCCCGAAAA GATAACCATT GCCGATTTGG CGCCAATGTA CCGCCAGTTT 
TATCGTGTGT TCGAAATCTT CAAGCTGGAA AACAAACCAA AGCCTGTGGA GAAGGACAAA 
TCTTCGCACG ATTCTGAGTC CCATCCCAGG GACAAAAAGG CTACAGACAA ACAGCTCGAG 
GACGAGGACG ATGACGGCGA TGATGACGAA GAGCGCAAGG AAGACAAGGA GAAGCTGTCC 
AAGCGGAAAC TTAAGAAACT GACCCGTTTG AGTGTGGCAG AACTGAAACA ACTGGTCTCT 
CGACCTGACG TAGTGGAGAT GCACGATGTC ACAGCGAGAG ATCCCAAACT ACTGGTTCAA 
CTGAAAGCCT ATAGGAACAC AGTACAAGTG CCGCGGCATT GGTGCTTCAA GCGAAAATAT 
CTGCAGGGCA AAAGGGGTAT TGAGAAGCCT CCGTTCGATC TCCCCGCCTT CATCAAAAAG 
ACGGGCATCA TGGAGATGCG CGAATCGTTG CAGGAACGAG AGGACGCCAA GACACTCAAG 
GCCAAGATGC GTGAACGCGT CCGTCCCAAG ATGGGCAAAA TTGACATTGA CTACCAGAAA 
CTTCACGATG CCTTCTTCAA GTGGCAGACC AAGCCCCGCA TGACCATTCA TGGCGACCTC 
TACTACGAGG GCAAGGAGTT TGAGACACGC CTTAAGGAAA AGAAGCCCGG AGATCTCTCC 
GAGGAGTTGC GCATTGCTCT GGGAATGCCC GTGGGACCCA ACTCACACAA GATTCCGCCA 
CCATGGCTCA TTGCTCAACA GCGCTACGGG CCGCCGCCCT CGTATCCGAA TCTTAAAATT 
CCTGGCCTGA ATGCTCCGAT CCCAGACGGA ACCTCGTTCG GTTATCATGC CGGAGGTTGG 
GGCAAGCCGC CAGTGGATGA GAACGGAAAA CCGCTGTACG GTGACGTATT TGGAACCAAC 
ATATTGGACC TTGATAATGG CGTAGACGAA GCTGACATTG AACGTAACCA ATGGGGCGAA 
CTGGAATCGG AGTCAGAAGA ATCATCCGAG GAGGAAGAGG AAGATGGCGA GGACTTGGGC 
GATCAGCAGG ATGAGACTGG CCTGGTAACG CCAGTAGAGG GACTAGTAAC ACCCTCTGGA 
CTCACCAGTG TTCCAGCTGG CATGGAAACC CCCGAGAACA TTGAGCTTCG CAAGAAGAAA 
ATTGAAGCCG AGATGGAGGA CAACGAAACT CCTGTTCTGT ATCAAGTGCT GCCTGAGAAG 
CGCACCGACC GCATAGGGGC TTCCATGATG GGATCAACCC ATGTCTACGA CGTCAGTGGC 
AGTGGGGCTA ACAAACAGCC CCCAGTTCGA TCAACCACAG ATCGCGAGGG CATTGTGGAA 
CTGGCTCTCG ATCCTAGCGA ATTGGACTTG GACAATGACG CAATGGCCCA GCGTTATGAG 
CAACAAATGC GTGAGCAGCA GAATCACCTG CAAAAGGAGG ATCTGTCAGA CATGTTGGCC 
GAACATGTGG CGCGGCAGAA ATCGAAGCGC AAGCGACAGC AAACGGATCC GGCCAAGACC 
ACAAAGAAGT ACAAGGAGTT CAAGTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Sf3b2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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