gammaTub23C cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	gammaTub23C
Entrez Gene ID	33501
Full Name	gamma-Tubulin at 23C
Synonyms	CG 3157,CG3157,D.m.GAMMA-23C,Dmel\CG3157,GAMMA23C,GTU88*,GTUB,Tub23,Tub3A,g-Tubulin,gamma tubulin,gamma-Tub,gamma-Tub23C,gamma-tub,gamma-tub 23C,gamma-tub23,gamma-tub23C,gamma-tubulin,gammaTUB,gammaTUB23C,gammaTub,gammaTub23CD,gamma[zy],gammatub,gammatub23C,l(2)23Ce
General protein information	Preferred Names gamma-Tubulin at 23C Names CG3157 gene product from transcript CG3157-RA &ggr-tubulin 23C gamma-tubulin 23C-gamma-tubulin CG3157-PA gama-tubulin gamma tubulin gamma tubulin 23C gamma-Tubulin gamma-tubulin (23C) gamma-tubulin 23C gamma-tubulin(23C) gamma-tubulin-1 gamma-tubulin23C gammaTub23C-PA gammatubulin lethal (2) 23Ce tubulin
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 23C1-23C4

mRNA and Protein(s)

mRNA	Protein	Name
NM_057456.4	NP_476804.1	gamma-Tubulin at 23C

Pathways from BioSystems

Homologies

Homo sapiens (human)	TUBG2	NP_057521.1
	TUBG2	XP_002800551.1
Canis lupus familiaris (dog)	TUBG2	XP_003639321.1
Bos taurus (cattle)	TUBG2	NP_001032704.1
Rattus norvegicus (Norway rat)	Tubg2	NP_001178004.1
Drosophila melanogaster (fruit fly)	gammaTub23C	NP_476804.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007834	XP_317665.3
Pan troglodytes (chimpanzee)	TUBG2	XP_003315538.1
Mus musculus (house mouse)	Tubg2	NP_598789.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that interphase centrioles are closely associated with Sas-4, Spd-2, Polo kinase, Pericentrin-like protein (Dplp), Asterless (Asl), Plk4 kinase, Centrosomin (Cnn) and gamma-tubulin.
Title: Structured illumination of the interface between centriole and peri-centriolar material.

The genetic interactions between gammaTub23C and chromatin-remodeling mutations suggest that gamma-tubulin might have a role in regulating gene expression.
Title: gammaTub23C interacts genetically with brahma chromatin-remodeling complexes in Drosophila melanogaster.

This indicates a role for Dgrip84 in regulating the activity and/or the location of the gamma-tubulin complexes formed with gammaTub37CD and gammaTub23C.
Title: Distinct Dgrip84 isoforms correlate with distinct gamma-tubulins in Drosophila.

findings showed that the molecular requirements for a functional spindle checkpoint included components of the large gamma-tubulin ring complex; however, their localization at the centrosome and centrosome integrity were not essential for this function
Title: A centrosome-independent role for gamma-TuRC proteins in the spindle assembly checkpoint.

gamma-tubulin is required for female germ-cell proliferation and that the two gamma-tubulins present in Drosophila are functionally equivalent during female germ-cell development and oogenesis.
Title: gamma-Tubulin function during female germ-cell development and oogenesis in Drosophila.

The following gammaTub23C gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gammaTub23C cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa13578	NM_057456.4 Latest version!	Drosophila melanogaster gamma-Tubulin at 23C (gammaTub23C), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gammaTub23C ( NM_057456.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476804.1 Drosophila melanogaster gamma-Tubulin at 23C (gammaTub23C), mRNA.

Price & Availability↑

CloneID	OFa13578
Clone ID Related Accession (Same CDS sequence)	NM_057456.4
Accession Version	NM_057456.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1428bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	gamma-Tubulin at 23C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_057456.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCAAGTG AAATAATTAC TTTGCAGCTT GGGCAGTGCG GCAATCAAAT TGGCTTTGAG 
TTCTGGAAAA GATTGTGCCT GGAGCATGGC ATCTCCCCTA GCGGCGTGCT GGAGGACTTT 
GCCAATGATG GCTTGGACCG CAAGGATGTG TTCTTCTACC AGGCGGACGA CGACCACTAC 
ATACCCCGCG CGGTGCTCTT GGACTTGGAA CCGAGGGTGA TCAATACCAT AATGGGCTCG 
GTCTACTCCA AGCTCTACAA TCCGGAAAAT GTGTACCTAT CCAAGCACGG AGGCGGTGCG 
GGCAACAACT GGGCCTCAGG CTATAGCCAG GGCGAAAAGC TGCAAGAAGA GGTATTCGAC 
ATCATTGACC GTGAGGCAGA TGGAAGCGAT TCGTTGGAGG GCTTTATACT TTGCCATTCG 
ATTGCTGGCG GAACGGGATC GGGAATGGGC TCCTTTATCA TGGAGCGCCT GGCGGACCGC 
TATCCCAAGA AACTTATCCA AACATTCAGT GTGTTTCCCA ATCAGGACGA GATCAGTGAT 
GTAGTGGTGC AGCCATACAA CTCCATGCTC ACCCTGAAAC GTTTGACTAC GGCTGCGGAC 
AGTGTGGTTG TCCTGGACAA CACTGCTCTT AATCGTATTG CCTGCGATCG CTTGCACATT 
CAGAATCCCA GCTTTTCGCA GATCAACAAC CTGGTCTCCA CCATAATGAG CGTCAGCACC 
ACTACGCTGA GGTATCCATC CTACATGAAC AACAATCTCA TTGGGCTGAC GGCTCCTTTA 
ATCCCGACGC CCCAGCTGCA TTTTCTCATG ACAGGCTACA CGCCGCTGAC TAGCGATTCG 
GATATCCATA CCCAGCAGCT GGTTAATGTG CGAAAGACTA CTGTTCTAGA TGTAATGCGG 
CGTCTGTTGC AGCCCAAGAA CATGATGGTG TCCACTGGTC CCGACAAGTC AAATCATCAC 
TGCTACATCT CCATTCTAAA CATTATCCAG GGCGAGGTGG ATCCCACTCA AGTGCACAAG 
TCACTGCAGC GCATCCGCGA CCGCAAGATG GCCCAGTTCA TTCCCTGGGG CCCCACCTCT 
ATCCAGGTAG CCCTTTCCCG CTCATCGCCC TACGTGCAGA GCAACCATCG AGTGTCCGGA 
CTGATGCTGG CCAACCACAC CAGCATTTGC TCGCTTTTCG AGCGAGCTCT CAACCAGTAT 
GACAAGCTTC GCAAGCGCGG CGCCTTTCTT GACCAGTTTC GACGCGAGGA CATCTTCAAG 
GACGACCTCA ATGAGCTGGA CGAATCTCGC GAAACTGTTG ACTGCCTGGT GCAAGAGTAT 
GAGGCAGCCA CGCGTGAGGA CTACATGCAG TTTTCAGTGA AGCGGGGAAA TGGGCCAGTT 
GACTCCAAGT CGGAGGATAG TCGATCTGTG ACCAGCGCCG GTTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476804.1
CDS	169..1596
Translation	MPSEIITLQLGQCGNQIGFEFWKRLCLEHGISPSGVLEDFANDGLDRKDVFFYQADDDHYIPRAVLLDLEPRVINTIMGSVYSKLYNPENVYLSKHGGGAGNNWASGYSQGEKLQEEVFDIIDREADGSDSLEGFILCHSIAGGTGSGMGSFIMERLADRYPKKLIQTFSVFPNQDEISDVVVQPYNSMLTLKRLTTAADSVVVLDNTALNRIACDRLHIQNPSFSQINNLVSTIMSVSTTTLRYPSYMNNNLIGLTAPLIPTPQLHFLMTGYTPLTSDSDIHTQQLVNVRKTTVLDVMRRLLQPKNMMVSTGPDKSNHHCYISILNIIQGEVDPTQVHKSLQRIRDRKMAQFIPWGPTSIQVALSRSSPYVQSNHRVSGLMLANHTSICSLFERALNQYDKLRKRGAFLDQFRREDIFKDDLNELDESRETVDCLVQEYEAATREDYMQFSVKRGNGPVDSKSEDSRSVTSAGS

Target ORF information:

RefSeq Version	NM_057456.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster gamma-Tubulin at 23C (gammaTub23C), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057456.4

ORF Insert Sequence:

ATGCCAAGTG AAATAATTAC TTTGCAGCTT GGGCAGTGCG GCAATCAAAT TGGCTTTGAG 
TTCTGGAAAA GATTGTGCCT GGAGCATGGC ATCTCCCCTA GCGGCGTGCT GGAGGACTTT 
GCCAATGATG GCTTGGACCG CAAGGATGTG TTCTTCTACC AGGCGGACGA CGACCACTAC 
ATACCCCGCG CGGTGCTCTT GGACTTGGAA CCGAGGGTGA TCAATACCAT AATGGGCTCG 
GTCTACTCCA AGCTCTACAA TCCGGAAAAT GTGTACCTAT CCAAGCACGG AGGCGGTGCG 
GGCAACAACT GGGCCTCAGG CTATAGCCAG GGCGAAAAGC TGCAAGAAGA GGTATTCGAC 
ATCATTGACC GTGAGGCAGA TGGAAGCGAT TCGTTGGAGG GCTTTATACT TTGCCATTCG 
ATTGCTGGCG GAACGGGATC GGGAATGGGC TCCTTTATCA TGGAGCGCCT GGCGGACCGC 
TATCCCAAGA AACTTATCCA AACATTCAGT GTGTTTCCCA ATCAGGACGA GATCAGTGAT 
GTAGTGGTGC AGCCATACAA CTCCATGCTC ACCCTGAAAC GTTTGACTAC GGCTGCGGAC 
AGTGTGGTTG TCCTGGACAA CACTGCTCTT AATCGTATTG CCTGCGATCG CTTGCACATT 
CAGAATCCCA GCTTTTCGCA GATCAACAAC CTGGTCTCCA CCATAATGAG CGTCAGCACC 
ACTACGCTGA GGTATCCATC CTACATGAAC AACAATCTCA TTGGGCTGAC GGCTCCTTTA 
ATCCCGACGC CCCAGCTGCA TTTTCTCATG ACAGGCTACA CGCCGCTGAC TAGCGATTCG 
GATATCCATA CCCAGCAGCT GGTTAATGTG CGAAAGACTA CTGTTCTAGA TGTAATGCGG 
CGTCTGTTGC AGCCCAAGAA CATGATGGTG TCCACTGGTC CCGACAAGTC AAATCATCAC 
TGCTACATCT CCATTCTAAA CATTATCCAG GGCGAGGTGG ATCCCACTCA AGTGCACAAG 
TCACTGCAGC GCATCCGCGA CCGCAAGATG GCCCAGTTCA TTCCCTGGGG CCCCACCTCT 
ATCCAGGTAG CCCTTTCCCG CTCATCGCCC TACGTGCAGA GCAACCATCG AGTGTCCGGA 
CTGATGCTGG CCAACCACAC CAGCATTTGC TCGCTTTTCG AGCGAGCTCT CAACCAGTAT 
GACAAGCTTC GCAAGCGCGG CGCCTTTCTT GACCAGTTTC GACGCGAGGA CATCTTCAAG 
GACGACCTCA ATGAGCTGGA CGAATCTCGC GAAACTGTTG ACTGCCTGGT GCAAGAGTAT 
GAGGCAGCCA CGCGTGAGGA CTACATGCAG TTTTCAGTGA AGCGGGGAAA TGGGCCAGTT 
GACTCCAAGT CGGAGGATAG TCGATCTGTG ACCAGCGCCG GTTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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gammaTub23C cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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