aph-1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	aph-1
Entrez Gene ID	33467
Full Name	anterior pharynx defective 1
Synonyms	Aph-1,Aph1,CG2855,Dm APH-1,Dmel\CG2855,Pen-1,aph,aph1,dAph-1,l(2)23AB6,l(2)23Ag,pen-1
General protein information	Preferred Names anterior pharynx defective 1 Names CG2855 gene product from transcript CG2855-RB CG2855-PA CG2855-PB aph-1-PA aph-1-PB presenilin enhancer-1 presenilin stabilization factor
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 23A5-23A5

mRNA and Protein(s)

mRNA	Protein	Name
NM_001273034.1	NP_001259963.1	anterior pharynx defective 1, isoform B
NM_134866.3	NP_608710.1	anterior pharynx defective 1, isoform A

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Aph1a	NP_666246.1
Rattus norvegicus (Norway rat)	Aph1a	NP_001014277.1
Drosophila melanogaster (fruit fly)	aph-1	NP_001259963.1
Pan troglodytes (chimpanzee)	APH1A	XP_513772.1
Bos taurus (cattle)	APH1A	NP_001029842.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009816	XP_318923.2
Gallus gallus (chicken)	LOC431243	XP_428797.3
Danio rerio (zebrafish)	aph1b	NP_956409.1
Oryza sativa (rice)	Os02g0272600	NP_001046527.1
	APH1A	XP_001100088.1
Caenorhabditis elegans (roundworm)	aph-1	NP_492469.1
Homo sapiens (human)	APH1A	NP_001071096.1
Canis lupus familiaris (dog)	APH1	XP_003639673.1
Arabidopsis thaliana (thale cress)	AT2G31440	NP_565724.1
Xenopus tropicalis (tropical clawed frog)	aph1a	NP_001015843.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Aph-1 is required to promote cell survival in the wing imaginal disc; aph-1 mutant cells are lost either through cell death or because of a defect in cell proliferation
Title: Aph-1 is required to regulate Presenilin-mediated gamma-secretase activity and cell survival in Drosophila wing development.

the proper assembly of the Aph-1.nicastrin subcomplex with presenilin is the prerequisite for the trafficking as well as the enzymatic activity of the gamma-secretase complex and Aph-1 functions as a stabilizing scaffold in the assembly of this complex
Title: Aph-1 contributes to the stabilization and trafficking of the gamma-secretase complex through mechanisms involving intermolecular and intramolecular interactions.

Aph-1 and Nct may form a subcomplex that stabilizes the Psn holoprotein at an early step in gamma-secretase assembly.
Title: Different cofactor activities in gamma-secretase assembly: evidence for a nicastrin-Aph-1 subcomplex.

APH-1 stabilizes the presenilin holoprotein in the complex, whereas PEN-2 is required for endoproteolytic processing of presenilin and conferring gamma-secretase activity to the complex.
Title: The role of presenilin cofactors in the gamma-secretase complex.

The following aph-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the aph-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa13537	NM_134866.3 Latest version!	Drosophila melanogaster anterior pharynx defective 1 (aph-1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa13537	NM_001273034.1 Latest version!	Drosophila melanogaster anterior pharynx defective 1 (aph-1), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

aph-1 ( NM_001273034.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259963.1 Drosophila melanogaster anterior pharynx defective 1 (aph-1), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa13537
Clone ID Related Accession (Same CDS sequence)	NM_001273034.1 , NM_134866.3
Accession Version	NM_001273034.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	anterior pharynx defective 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGTTGC CCGAGTTCTT TGGCTGCACC TTCATCGCCT TCGGACCGCC CTTCGCCTTG 
TTCGTCTTCA CCATCGCCAA TGATCCAGTG CGGATCATCA TTCTGATTGC GGCGGCATTC 
TTCTGGCTGC TTTCCCTGCT GATCTCTTCC CTGTGGTATG CCCTGATTCC GCTGAAGGAG 
TTCCTGGCAT TTGGCGTGGT CTTCTCGGTG TGCTTCCAGG AAGCCTTCCG GTACATCATC 
TACCGGATAC TGCGCAGCAC GGAGCAGGGA TTGCACGCCG TGGCGGAGGA CACGCGAGTG 
ACGGACAACA AGCACATCCT GGCCTATGTC TCCGGCTTGG GATTCGGCAT TATATCCGGG 
ATGTTTGCAC TGGTCAATGT GCTGGCTGAT ATGAGTGGTC CCGGCACCAT GGGCTTGAAG 
GGCGGAACTG AGCTATTCTT CGTCACCTCG GCTGCCCAGG CGTTGTCGAT TATCCTGCTG 
CACACCTTCT GGAGCGTTAT TTTCTTCAAC GCATTCGACA CAAACAACTA TATCCACATA 
GGCTATGTGG TTTTCAGCCA CCTGTTCGTC TCCCTGATAA CTCTGCTCAA TGCCAATGAG 
CTTTACACGA CCACTCTGCT GATAAACTAC TTGGTCACCA TACTTACGGG AGTCCTGGCC 
TTCCGGGTGG CTGGAGGAAC ATCTCGCAGT TTCAGAAAAT TCATAACATG CCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259963.1
CDS	83..799
Translation	MTLPEFFGCTFIAFGPPFALFVFTIANDPVRIIILIAAAFFWLLSLLISSLWYALIPLKEFLAFGVVFSVCFQEAFRYIIYRILRSTEQGLHAVAEDTRVTDNKHILAYVSGLGFGIISGMFALVNVLADMSGPGTMGLKGGTELFFVTSAAQALSIILLHTFWSVIFFNAFDTNNYIHIGYVVFSHLFVSLITLLNANELYTTTLLINYLVTILTGVLAFRVAGGTSRSFRKFITCQ

Target ORF information:

RefSeq Version	NM_001273034.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster anterior pharynx defective 1 (aph-1), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273034.1

ORF Insert Sequence:

ATGACGTTGC CCGAGTTCTT TGGCTGCACC TTCATCGCCT TCGGACCGCC CTTCGCCTTG 
TTCGTCTTCA CCATCGCCAA TGATCCAGTG CGGATCATCA TTCTGATTGC GGCGGCATTC 
TTCTGGCTGC TTTCCCTGCT GATCTCTTCC CTGTGGTATG CCCTGATTCC GCTGAAGGAG 
TTCCTGGCAT TTGGCGTGGT CTTCTCGGTG TGCTTCCAGG AAGCCTTCCG GTACATCATC 
TACCGGATAC TGCGCAGCAC GGAGCAGGGA TTGCACGCCG TGGCGGAGGA CACGCGAGTG 
ACGGACAACA AGCACATCCT GGCCTATGTC TCCGGCTTGG GATTCGGCAT TATATCCGGG 
ATGTTTGCAC TGGTCAATGT GCTGGCTGAT ATGAGTGGTC CCGGCACCAT GGGCTTGAAG 
GGCGGAACTG AGCTATTCTT CGTCACCTCG GCTGCCCAGG CGTTGTCGAT TATCCTGCTG 
CACACCTTCT GGAGCGTTAT TTTCTTCAAC GCATTCGACA CAAACAACTA TATCCACATA 
GGCTATGTGG TTTTCAGCCA CCTGTTCGTC TCCCTGATAA CTCTGCTCAA TGCCAATGAG 
CTTTACACGA CCACTCTGCT GATAAACTAC TTGGTCACCA TACTTACGGG AGTCCTGGCC 
TTCCGGGTGG CTGGAGGAAC ATCTCGCAGT TTCAGAAAAT TCATAACATG CCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

aph-1 ( NM_134866.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_608710.1 Drosophila melanogaster anterior pharynx defective 1 (aph-1), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa13537
Clone ID Related Accession (Same CDS sequence)	NM_001273034.1 , NM_134866.3
Accession Version	NM_134866.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	anterior pharynx defective 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_134866.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGTTGC CCGAGTTCTT TGGCTGCACC TTCATCGCCT TCGGACCGCC CTTCGCCTTG 
TTCGTCTTCA CCATCGCCAA TGATCCAGTG CGGATCATCA TTCTGATTGC GGCGGCATTC 
TTCTGGCTGC TTTCCCTGCT GATCTCTTCC CTGTGGTATG CCCTGATTCC GCTGAAGGAG 
TTCCTGGCAT TTGGCGTGGT CTTCTCGGTG TGCTTCCAGG AAGCCTTCCG GTACATCATC 
TACCGGATAC TGCGCAGCAC GGAGCAGGGA TTGCACGCCG TGGCGGAGGA CACGCGAGTG 
ACGGACAACA AGCACATCCT GGCCTATGTC TCCGGCTTGG GATTCGGCAT TATATCCGGG 
ATGTTTGCAC TGGTCAATGT GCTGGCTGAT ATGAGTGGTC CCGGCACCAT GGGCTTGAAG 
GGCGGAACTG AGCTATTCTT CGTCACCTCG GCTGCCCAGG CGTTGTCGAT TATCCTGCTG 
CACACCTTCT GGAGCGTTAT TTTCTTCAAC GCATTCGACA CAAACAACTA TATCCACATA 
GGCTATGTGG TTTTCAGCCA CCTGTTCGTC TCCCTGATAA CTCTGCTCAA TGCCAATGAG 
CTTTACACGA CCACTCTGCT GATAAACTAC TTGGTCACCA TACTTACGGG AGTCCTGGCC 
TTCCGGGTGG CTGGAGGAAC ATCTCGCAGT TTCAGAAAAT TCATAACATG CCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_608710.1
CDS	83..799
Translation	MTLPEFFGCTFIAFGPPFALFVFTIANDPVRIIILIAAAFFWLLSLLISSLWYALIPLKEFLAFGVVFSVCFQEAFRYIIYRILRSTEQGLHAVAEDTRVTDNKHILAYVSGLGFGIISGMFALVNVLADMSGPGTMGLKGGTELFFVTSAAQALSIILLHTFWSVIFFNAFDTNNYIHIGYVVFSHLFVSLITLLNANELYTTTLLINYLVTILTGVLAFRVAGGTSRSFRKFITCQ

Target ORF information:

RefSeq Version	NM_134866.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster anterior pharynx defective 1 (aph-1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134866.3

ORF Insert Sequence:

ATGACGTTGC CCGAGTTCTT TGGCTGCACC TTCATCGCCT TCGGACCGCC CTTCGCCTTG 
TTCGTCTTCA CCATCGCCAA TGATCCAGTG CGGATCATCA TTCTGATTGC GGCGGCATTC 
TTCTGGCTGC TTTCCCTGCT GATCTCTTCC CTGTGGTATG CCCTGATTCC GCTGAAGGAG 
TTCCTGGCAT TTGGCGTGGT CTTCTCGGTG TGCTTCCAGG AAGCCTTCCG GTACATCATC 
TACCGGATAC TGCGCAGCAC GGAGCAGGGA TTGCACGCCG TGGCGGAGGA CACGCGAGTG 
ACGGACAACA AGCACATCCT GGCCTATGTC TCCGGCTTGG GATTCGGCAT TATATCCGGG 
ATGTTTGCAC TGGTCAATGT GCTGGCTGAT ATGAGTGGTC CCGGCACCAT GGGCTTGAAG 
GGCGGAACTG AGCTATTCTT CGTCACCTCG GCTGCCCAGG CGTTGTCGAT TATCCTGCTG 
CACACCTTCT GGAGCGTTAT TTTCTTCAAC GCATTCGACA CAAACAACTA TATCCACATA 
GGCTATGTGG TTTTCAGCCA CCTGTTCGTC TCCCTGATAA CTCTGCTCAA TGCCAATGAG 
CTTTACACGA CCACTCTGCT GATAAACTAC TTGGTCACCA TACTTACGGG AGTCCTGGCC 
TTCCGGGTGG CTGGAGGAAC ATCTCGCAGT TTCAGAAAAT TCATAACATG CCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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aph-1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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