upd3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	upd3
Entrez Gene ID	3346149
Full Name	unpaired 3
Synonyms	CG15062,CG15062/CG5963,CG33542,CG5963,Dmel\CG33542,UPD3,Upd-3,Upd-like,Upd3,cg15062,cg5963,od,odsy,os,sy,upd-3
General protein information	Preferred Names unpaired 3 Names CG33542 gene product from transcript CG33542-RB CG33542-PB Unpaired3 outstretched outstretched-smalleye small-eye unpaired unpaired 3beta unpaired-3 unpaired-like protein 3 upd homolog upd3-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 17A4-17A4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001103544.2	NP_001097014.1	unpaired 3

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Infection-induced upd3 expression in enterocytes requires the indirect functions of snail and its transcriptional co-repressors, as well as homeodomain transcription factors and epigenetic regulators. Upd3 transcription is regulated by a combination of microbe-responsive, cell-specific and region-specific enhancers.
Title: Hippo, TGF-?, and Src-MAPK pathways regulate transcription of the upd3 cytokine in Drosophila enterocytes upon bacterial infection.

Our study shows that haemocytes express the three upd genes and that injury markedly induces the expression of upd3 by the JNK pathway in haemocytes, which in turn activates the JAK/STAT pathway in the fat body and the gut. Surprisingly, release of Upd3 from haemocytes upon injury can remotely stimulate stem cell proliferation and the expression of Drosomycin-like genes in the intestine.
Title: Remote Control of Intestinal Stem Cell Activity by Haemocytes in Drosophila.

controls glucose metabolism and reduces lifespan on a lipid-rich diet via activation of the JAK-STAT pathway
Title: Macrophage-derived upd3 cytokine causes impaired glucose homeostasis and reduced lifespan in Drosophila fed a lipid-rich diet.

unpaired 3 is essential for a subset of JAK-mediated developmental functions.
Title: Pleiotropy of the Drosophila JAK pathway cytokine Unpaired 3 in development and aging.

Drosophila protein Upd3 is related to vertebrate IL-6 and has a role as a monomeric-alpha helical cytokine, an ancestor of the four-helix bundle cytokines
Title: Upd3--an ancestor of the four-helix bundle cytokines.

The following upd3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the upd3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa02565	NM_001103544.2 Latest version!	Drosophila melanogaster unpaired 3 (upd3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

upd3 ( NM_001103544.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097014.1 Drosophila melanogaster unpaired 3 (upd3), mRNA.

Price & Availability↑

CloneID	OFa02565
Clone ID Related Accession (Same CDS sequence)	NM_001103544.2
Accession Version	NM_001103544.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1206bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	unpaired 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001103544.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGACAG CTGACCGCCC GCTCGACGTC AGCAGGAGCA GCGGAAGCGG TAGCCGGAGC 
GGTAACAAAA CGGCCAGAAC CAGGAATCCA GTGGAACCGC AGCCGCAACC GCCGTTCGTG 
GATCGACGAC GACGCCTGAA CAGGAACTGG TCACTGATCT TACTCGCCTT GGCCATGTCC 
CAGTTTGCCC TCTCCGGCGG CGGTTGTGGC GTCTCAGCGG CGCCCAGTCA CCTGGCCACC 
CCGCCCACTG ACAGCCCCTC CAGTCCCATC AATCCCACCA ATCCCCTGAA GCACCTACAG 
AAGCGTTCCA GAGCGGCCAA CTTCCGGCTG ACCTTCCAGC AGAAATTGAA TGCCAGCAGT 
ACGCATCTGG ACTGGGAGAA CACCTGCAAT CTGAAGCCCA CGGGTCTGAA CGAAACGCAC 
AGCAAGGCGA AACGCTGCAA GAAACGCCAA AGGATCCTGC AGAATCTACA GAACCAAACG 
GGCCGCGAGC TGCGGGGCAT CCAGGCCGAG GACAAGGCCA GGATCACCAC CAATGCGGAC 
AAGCTGGCCA CAGTGAGCAC CAAGACTCTG GACATTGTCG AGAAGAACAA GTGGCGATTC 
TATAAGGGAA ACTACAGATT CCTGCCCCGT CTGAATCTCA CTAGCAAACA GTTGAACCTT 
CGCCACGCCC ATCGCGACCT GCAGATTTAC GTGGGCGCCT TCACGTACAT GCGCAACATC 
AAGTTGCACT GGGACCTGGC CAACCTGCAG GCGAAGAGCG TGCTCTCCGA CGAGCTGGGA 
AGGATGCGGA AGTCCGCCCG CGAGGTGCTC TGCAGCGTGG AGGAGGCCAT CAACCTGACC 
AACCTGCTGT ACACACCCAA CAGGCAGCGG GCGCCACAGC CGAGGGGCCA GAAGAAGCGG 
CGCCAGGCCC AACCGGTGGT CACCTACAAG ATACTGCCGC GCAAGCTGAT GGAGAAGCGT 
CTGCAGCAGT TCAACTCGAC GTTGGTGCCA CTCGTGGAGC TGCACCAGCA GGCGACCTTG 
GCCGCACGCG GGGAGGATGT ACCACCACCG CCCGACGACC TGATGGTCCT GGAGCAGCAC 
GCCCTCTTCA CCAAACTGAA GTTTGTGCAG TATCTCAAGA GTATCCGCAA GATACTCGCG 
AACCAGCGTA AGAGTCTGTG CAAGGCGACC ACCCACGTGC CAAAGGCGAT CCAGAAGAAA 
CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097014.1
CDS	371..1576
Translation	MTTADRPLDVSRSSGSGSRSGNKTARTRNPVEPQPQPPFVDRRRRLNRNWSLILLALAMSQFALSGGGCGVSAAPSHLATPPTDSPSSPINPTNPLKHLQKRSRAANFRLTFQQKLNASSTHLDWENTCNLKPTGLNETHSKAKRCKKRQRILQNLQNQTGRELRGIQAEDKARITTNADKLATVSTKTLDIVEKNKWRFYKGNYRFLPRLNLTSKQLNLRHAHRDLQIYVGAFTYMRNIKLHWDLANLQAKSVLSDELGRMRKSAREVLCSVEEAINLTNLLYTPNRQRAPQPRGQKKRRQAQPVVTYKILPRKLMEKRLQQFNSTLVPLVELHQQATLAARGEDVPPPPDDLMVLEQHALFTKLKFVQYLKSIRKILANQRKSLCKATTHVPKAIQKKL

Target ORF information:

RefSeq Version	NM_001103544.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster unpaired 3 (upd3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103544.2

ORF Insert Sequence:

ATGACGACAG CTGACCGCCC GCTCGACGTC AGCAGGAGCA GCGGAAGCGG TAGCCGGAGC 
GGTAACAAAA CGGCCAGAAC CAGGAATCCA GTGGAACCGC AGCCGCAACC GCCGTTCGTG 
GATCGACGAC GACGCCTGAA CAGGAACTGG TCACTGATCT TACTCGCCTT GGCCATGTCC 
CAGTTTGCCC TCTCCGGCGG CGGTTGTGGC GTCTCAGCGG CGCCCAGTCA CCTGGCCACC 
CCGCCCACTG ACAGCCCCTC CAGTCCCATC AATCCCACCA ATCCCCTGAA GCACCTACAG 
AAGCGTTCCA GAGCGGCCAA CTTCCGGCTG ACCTTCCAGC AGAAATTGAA TGCCAGCAGT 
ACGCATCTGG ACTGGGAGAA CACCTGCAAT CTGAAGCCCA CGGGTCTGAA CGAAACGCAC 
AGCAAGGCGA AACGCTGCAA GAAACGCCAA AGGATCCTGC AGAATCTACA GAACCAAACG 
GGCCGCGAGC TGCGGGGCAT CCAGGCCGAG GACAAGGCCA GGATCACCAC CAATGCGGAC 
AAGCTGGCCA CAGTGAGCAC CAAGACTCTG GACATTGTCG AGAAGAACAA GTGGCGATTC 
TATAAGGGAA ACTACAGATT CCTGCCCCGT CTGAATCTCA CTAGCAAACA GTTGAACCTT 
CGCCACGCCC ATCGCGACCT GCAGATTTAC GTGGGCGCCT TCACGTACAT GCGCAACATC 
AAGTTGCACT GGGACCTGGC CAACCTGCAG GCGAAGAGCG TGCTCTCCGA CGAGCTGGGA 
AGGATGCGGA AGTCCGCCCG CGAGGTGCTC TGCAGCGTGG AGGAGGCCAT CAACCTGACC 
AACCTGCTGT ACACACCCAA CAGGCAGCGG GCGCCACAGC CGAGGGGCCA GAAGAAGCGG 
CGCCAGGCCC AACCGGTGGT CACCTACAAG ATACTGCCGC GCAAGCTGAT GGAGAAGCGT 
CTGCAGCAGT TCAACTCGAC GTTGGTGCCA CTCGTGGAGC TGCACCAGCA GGCGACCTTG 
GCCGCACGCG GGGAGGATGT ACCACCACCG CCCGACGACC TGATGGTCCT GGAGCAGCAC 
GCCCTCTTCA CCAAACTGAA GTTTGTGCAG TATCTCAAGA GTATCCGCAA GATACTCGCG 
AACCAGCGTA AGAGTCTGTG CAAGGCGACC ACCCACGTGC CAAAGGCGAT CCAGAAGAAA 
CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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upd3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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