VGlut cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	VGlut
Entrez Gene ID	33427
Full Name	Vesicular glutamate transporter
Synonyms	CG9887,DV-Glut,DVGLUT,DVGluT,DVGlut,Dmel\CG9887,DvGluT,DvGlut,MFS11,VGLUT,VGluT,Vglut,dVGLUT,dVGlut,dvGluT,dvGlut,dvglut,vGLUT,vGluT,vGlut,vglut
General protein information	Preferred Names Vesicular glutamate transporter Names CG9887 gene product from transcript CG9887-RC CG9887-PA CG9887-PB CG9887-PC VGlut-PA VGlut-PB VGlut-PC drosophila vesicular glutamate transporter vesicular glutamate transporter
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 22E1-22E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_134837.3	NP_608681.2	vesicular glutamate transporter, isoform A
NM_001273010.1	NP_001259939.1	vesicular glutamate transporter, isoform C
NM_001258919.1	NP_001245848.1	vesicular glutamate transporter, isoform B

Pathways from BioSystems

Homologies

	SLC17A6	XP_002799649.1
Gallus gallus (chicken)	SLC17A6	NP_001161855.1
Pan troglodytes (chimpanzee)	SLC17A6	XP_003313009.1
Canis lupus familiaris (dog)	SLC17A6	NP_001161886.1
Danio rerio (zebrafish)	slc17a6a	NP_001009982.1
Drosophila melanogaster (fruit fly)	VGlut	NP_608681.2
Xenopus tropicalis (tropical clawed frog)	slc17a6	XP_004913390.1
Homo sapiens (human)	SLC17A6	NP_065079.1
Mus musculus (house mouse)	Slc17a6	NP_543129.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007992	XP_317474.4
Rattus norvegicus (Norway rat)	Slc17a6	NP_445879.1
Bos taurus (cattle)	SLC17A6	NP_001096580.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study demonstrated that Astrocytic glutamate transport regulates a Drosophila CNS synapse that lacks astrocyte ensheathment.
Title: Astrocytic glutamate transport regulates a Drosophila CNS synapse that lacks astrocyte ensheathment.

Overexpression of Drosophila GLUT is sufficient to generate excitotoxic neuropathological phenotypes; therefore, reducing VGLUT levels after nervous system injury or stress may mitigate further damage.
Title: Increased vesicular glutamate transporter expression causes excitotoxic neurodegeneration.

In the larval CNS,synaptic DVGLUT in all brain lobe neuropil compartments except for the mushroom body. Likewise in the adult CNS, glutamatergic synapses are abundant throughout all major brain structures except the mushroom body.
Title: Visualizing glutamatergic cell bodies and synapses in Drosophila larval and adult CNS.

DVGLUT contains 10 hydrophobic regions that completely span the membrane (TMs 1-10) & that the amino & carboxyl termini are cytosolic. between TMs 4 & 5 is an unforeseen cytosolic loop of some 50 residues.
Title: Membrane topology of the Drosophila vesicular glutamate transporter.

The following VGlut gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VGlut cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa13501	NM_001273010.1 Latest version!	Drosophila melanogaster vesicular glutamate transporter, transcript variant C (VGlut), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa13501	NM_134837.3 Latest version!	Drosophila melanogaster vesicular glutamate transporter, transcript variant A (VGlut), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa13501	NM_001258919.1 Latest version!	Drosophila melanogaster vesicular glutamate transporter, transcript variant B (VGlut), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VGlut ( NM_134837.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_608681.2 Drosophila melanogaster vesicular glutamate transporter, transcript variant A (VGlut), mRNA.

Price & Availability↑

CloneID	OFa13501
Clone ID Related Accession (Same CDS sequence)	NM_134837.3 , NM_001273010.1 , NM_001258919.1
Accession Version	NM_134837.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1899bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vesicular glutamate transporter, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On May 8, 2012 this sequence version replaced NM_134837.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGGGTC TGACGGCGTT TAAGGAGAAG GCAACGGGCG TGTTTGGCGG CCTGAAGCCC 
AATATGGAGA AGTTTGAGAT ATCGCAGAGC TATCATGGTG GCCACGGTGG CTACGAGGAA 
ATGGAGGGGG GCGACCGGGA GGGCCGCGGA CCAGGCGGCG GCCACGCCTA CGACGACGAT 
GACGACCGGC CCGACTCGCC CGCCTCCTTC GAGGAGATCG AGCGCCCGCC GCTCCGCAAG 
ATCGACAAGT ACTGCAAGGC GGAGTGTCCT TGCATGCCGG CCCGCTACAC CATCGCCACC 
ATGGCCTGCG TGGGATTCAT GATCGCCTTC GGCATGAGGT GCAATATGTC GGCGGCCAAG 
CTCAAAGGGG AGCACAATGG GACTGTGTTC ATGAACTGGA CGGTCGCCGT GGAGAGCCAT 
GTGGACTCGT CCTTCTTCTG GGGCTATCTG GTGACGCAGA TTCCCGGCGG CTTCATCGCC 
TCCAAGTTCC CGGCCAACAA GATATTCGGA CTGTCCATCG TGAGCTCCGC CACGCTGCAT 
CTCTTCGTGC CATTCGCCAT GACCCTGATG CACGGTCATG TGGTGATTTG CGTGAGGGTC 
CTGCAAGGAC TCTTCGAGGG CGTTACCTAT CCAGCTTGCC ATGGTATCTG GCGTTTCTGG 
GCGCCGCCCA TGGAGCGCTC CCGACTGGCG ACGCTGGCCT TTTCCGGTTC CTATGCGGGC 
GTGGTGGTTG GACTTCCGCT CTCCGGACTT CTGGCCGATG CCGTGGGCTA CCAGGCGCCG 
TTCTACGCCT ACGGGGTGTT CGGAATCATA TGGTACATGT TCTGGATATG GTTGTGCTTC 
GAGAACCCGC GCAAACATCC GGCCATCAGC ATACCCGAGT TGAAGTACAT CGAGAAATCG 
CTCGGGGAGT CGGCTCATCC CACGATGCCA TCCCTGAAGA CGACTCCGTG GCGGGAAATG 
ATGCGCTCGA TGCCGGTCTA CGCCATCATT GTGGCCAACT TCTGCCGCTC CTGGAACTTC 
TACCTCCTGG TGCTGTTCCA GTCCTCGTTC CTCAAGCACA AGTTCGGTTT TAAGGTGGAG 
GAGGCGGGCT TCGTGGGCTC GCTGCCCCAC TTGATCATGA CTACGATAGT TCCATTTGGC 
GGCATGTTGG CGGATCACCT GCGAAAGAAT GGTATCCTGT CCACCACCAA TGTGCGCAAG 
CTCTTCAATT GCGGCGGCTT TGGCATGGAG GGTCTGTTTT TCCTATTCGT GGCACATTCC 
TCAACGGCGA CGGGTGCCAT GTTTGCCTTG ACCTGCGGCG TGGCCTTCAG TGGCTTTGCC 
ATATCCGGTT ATAATGTCAA TCACCTGGAT ATTGCTCCTC GTTATGCTAG TATATTGATG 
GGTCTTTCGA ATGGAATTGG TACTCTGGCC GGCATCATTG TGCCCTATGC CCTTGATGGC 
CTCATCCAAG CTAATCCTAC CGGGTGTTGG ACTACAGTCT TCACCCTGGC CGCCTGTGTT 
CATTTGGTTG GCTGCACTTT CTATGGTATT TTCGCATCTG GAGAGCTGCA GCCGTGGGCG 
GAACCTCCGG CCGAGGAGCA AAAGGTGTGG GCTCCACCAC CAGGTGCCAT TACCAACACG 
GATCCTAGCC AGGCGGGCAT GTTGGGCGAC TACATGAAGG AAACCTCATT CGGTGCCCCC 
GAGTACACTG AGCAGAGCCA AATGCAGCAG TCGACTGCCA TTAGCTACGG CGCCACAGGA 
CACGTGGCCA ACAATCCCTT CGCCATGGCC AGTGGTGCTC CGCCCATTGC GGAGGAGGAT 
GCCCCACCGA CCTACGGGGA TGTTACCAAT CCTGGGCAGT ATGGCTACAC GCAAGGACAA 
ATGCCGTCCT ACGATCCGCA GGGATACCAG CAGCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_608681.2
CDS	639..2537
Translation	MKGLTAFKEKATGVFGGLKPNMEKFEISQSYHGGHGGYEEMEGGDREGRGPGGGHAYDDDDDRPDSPASFEEIERPPLRKIDKYCKAECPCMPARYTIATMACVGFMIAFGMRCNMSAAKLKGEHNGTVFMNWTVAVESHVDSSFFWGYLVTQIPGGFIASKFPANKIFGLSIVSSATLHLFVPFAMTLMHGHVVICVRVLQGLFEGVTYPACHGIWRFWAPPMERSRLATLAFSGSYAGVVVGLPLSGLLADAVGYQAPFYAYGVFGIIWYMFWIWLCFENPRKHPAISIPELKYIEKSLGESAHPTMPSLKTTPWREMMRSMPVYAIIVANFCRSWNFYLLVLFQSSFLKHKFGFKVEEAGFVGSLPHLIMTTIVPFGGMLADHLRKNGILSTTNVRKLFNCGGFGMEGLFFLFVAHSSTATGAMFALTCGVAFSGFAISGYNVNHLDIAPRYASILMGLSNGIGTLAGIIVPYALDGLIQANPTGCWTTVFTLAACVHLVGCTFYGIFASGELQPWAEPPAEEQKVWAPPPGAITNTDPSQAGMLGDYMKETSFGAPEYTEQSQMQQSTAISYGATGHVANNPFAMASGAPPIAEEDAPPTYGDVTNPGQYGYTQGQMPSYDPQGYQQQ

Target ORF information:

RefSeq Version	NM_134837.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vesicular glutamate transporter, transcript variant A (VGlut), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134837.3

ORF Insert Sequence:

ATGAAGGGTC TGACGGCGTT TAAGGAGAAG GCAACGGGCG TGTTTGGCGG CCTGAAGCCC 
AATATGGAGA AGTTTGAGAT ATCGCAGAGC TATCATGGTG GCCACGGTGG CTACGAGGAA 
ATGGAGGGGG GCGACCGGGA GGGCCGCGGA CCAGGCGGCG GCCACGCCTA CGACGACGAT 
GACGACCGGC CCGACTCGCC CGCCTCCTTC GAGGAGATCG AGCGCCCGCC GCTCCGCAAG 
ATCGACAAGT ACTGCAAGGC GGAGTGTCCT TGCATGCCGG CCCGCTACAC CATCGCCACC 
ATGGCCTGCG TGGGATTCAT GATCGCCTTC GGCATGAGGT GCAATATGTC GGCGGCCAAG 
CTCAAAGGGG AGCACAATGG GACTGTGTTC ATGAACTGGA CGGTCGCCGT GGAGAGCCAT 
GTGGACTCGT CCTTCTTCTG GGGCTATCTG GTGACGCAGA TTCCCGGCGG CTTCATCGCC 
TCCAAGTTCC CGGCCAACAA GATATTCGGA CTGTCCATCG TGAGCTCCGC CACGCTGCAT 
CTCTTCGTGC CATTCGCCAT GACCCTGATG CACGGTCATG TGGTGATTTG CGTGAGGGTC 
CTGCAAGGAC TCTTCGAGGG CGTTACCTAT CCAGCTTGCC ATGGTATCTG GCGTTTCTGG 
GCGCCGCCCA TGGAGCGCTC CCGACTGGCG ACGCTGGCCT TTTCCGGTTC CTATGCGGGC 
GTGGTGGTTG GACTTCCGCT CTCCGGACTT CTGGCCGATG CCGTGGGCTA CCAGGCGCCG 
TTCTACGCCT ACGGGGTGTT CGGAATCATA TGGTACATGT TCTGGATATG GTTGTGCTTC 
GAGAACCCGC GCAAACATCC GGCCATCAGC ATACCCGAGT TGAAGTACAT CGAGAAATCG 
CTCGGGGAGT CGGCTCATCC CACGATGCCA TCCCTGAAGA CGACTCCGTG GCGGGAAATG 
ATGCGCTCGA TGCCGGTCTA CGCCATCATT GTGGCCAACT TCTGCCGCTC CTGGAACTTC 
TACCTCCTGG TGCTGTTCCA GTCCTCGTTC CTCAAGCACA AGTTCGGTTT TAAGGTGGAG 
GAGGCGGGCT TCGTGGGCTC GCTGCCCCAC TTGATCATGA CTACGATAGT TCCATTTGGC 
GGCATGTTGG CGGATCACCT GCGAAAGAAT GGTATCCTGT CCACCACCAA TGTGCGCAAG 
CTCTTCAATT GCGGCGGCTT TGGCATGGAG GGTCTGTTTT TCCTATTCGT GGCACATTCC 
TCAACGGCGA CGGGTGCCAT GTTTGCCTTG ACCTGCGGCG TGGCCTTCAG TGGCTTTGCC 
ATATCCGGTT ATAATGTCAA TCACCTGGAT ATTGCTCCTC GTTATGCTAG TATATTGATG 
GGTCTTTCGA ATGGAATTGG TACTCTGGCC GGCATCATTG TGCCCTATGC CCTTGATGGC 
CTCATCCAAG CTAATCCTAC CGGGTGTTGG ACTACAGTCT TCACCCTGGC CGCCTGTGTT 
CATTTGGTTG GCTGCACTTT CTATGGTATT TTCGCATCTG GAGAGCTGCA GCCGTGGGCG 
GAACCTCCGG CCGAGGAGCA AAAGGTGTGG GCTCCACCAC CAGGTGCCAT TACCAACACG 
GATCCTAGCC AGGCGGGCAT GTTGGGCGAC TACATGAAGG AAACCTCATT CGGTGCCCCC 
GAGTACACTG AGCAGAGCCA AATGCAGCAG TCGACTGCCA TTAGCTACGG CGCCACAGGA 
CACGTGGCCA ACAATCCCTT CGCCATGGCC AGTGGTGCTC CGCCCATTGC GGAGGAGGAT 
GCCCCACCGA CCTACGGGGA TGTTACCAAT CCTGGGCAGT ATGGCTACAC GCAAGGACAA 
ATGCCGTCCT ACGATCCGCA GGGATACCAG CAGCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VGlut ( NM_001273010.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259939.1 Drosophila melanogaster vesicular glutamate transporter, transcript variant C (VGlut), mRNA.

Price & Availability↑

CloneID	OFa13501
Clone ID Related Accession (Same CDS sequence)	NM_134837.3 , NM_001273010.1 , NM_001258919.1
Accession Version	NM_001273010.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1899bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vesicular glutamate transporter, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGGGTC TGACGGCGTT TAAGGAGAAG GCAACGGGCG TGTTTGGCGG CCTGAAGCCC 
AATATGGAGA AGTTTGAGAT ATCGCAGAGC TATCATGGTG GCCACGGTGG CTACGAGGAA 
ATGGAGGGGG GCGACCGGGA GGGCCGCGGA CCAGGCGGCG GCCACGCCTA CGACGACGAT 
GACGACCGGC CCGACTCGCC CGCCTCCTTC GAGGAGATCG AGCGCCCGCC GCTCCGCAAG 
ATCGACAAGT ACTGCAAGGC GGAGTGTCCT TGCATGCCGG CCCGCTACAC CATCGCCACC 
ATGGCCTGCG TGGGATTCAT GATCGCCTTC GGCATGAGGT GCAATATGTC GGCGGCCAAG 
CTCAAAGGGG AGCACAATGG GACTGTGTTC ATGAACTGGA CGGTCGCCGT GGAGAGCCAT 
GTGGACTCGT CCTTCTTCTG GGGCTATCTG GTGACGCAGA TTCCCGGCGG CTTCATCGCC 
TCCAAGTTCC CGGCCAACAA GATATTCGGA CTGTCCATCG TGAGCTCCGC CACGCTGCAT 
CTCTTCGTGC CATTCGCCAT GACCCTGATG CACGGTCATG TGGTGATTTG CGTGAGGGTC 
CTGCAAGGAC TCTTCGAGGG CGTTACCTAT CCAGCTTGCC ATGGTATCTG GCGTTTCTGG 
GCGCCGCCCA TGGAGCGCTC CCGACTGGCG ACGCTGGCCT TTTCCGGTTC CTATGCGGGC 
GTGGTGGTTG GACTTCCGCT CTCCGGACTT CTGGCCGATG CCGTGGGCTA CCAGGCGCCG 
TTCTACGCCT ACGGGGTGTT CGGAATCATA TGGTACATGT TCTGGATATG GTTGTGCTTC 
GAGAACCCGC GCAAACATCC GGCCATCAGC ATACCCGAGT TGAAGTACAT CGAGAAATCG 
CTCGGGGAGT CGGCTCATCC CACGATGCCA TCCCTGAAGA CGACTCCGTG GCGGGAAATG 
ATGCGCTCGA TGCCGGTCTA CGCCATCATT GTGGCCAACT TCTGCCGCTC CTGGAACTTC 
TACCTCCTGG TGCTGTTCCA GTCCTCGTTC CTCAAGCACA AGTTCGGTTT TAAGGTGGAG 
GAGGCGGGCT TCGTGGGCTC GCTGCCCCAC TTGATCATGA CTACGATAGT TCCATTTGGC 
GGCATGTTGG CGGATCACCT GCGAAAGAAT GGTATCCTGT CCACCACCAA TGTGCGCAAG 
CTCTTCAATT GCGGCGGCTT TGGCATGGAG GGTCTGTTTT TCCTATTCGT GGCACATTCC 
TCAACGGCGA CGGGTGCCAT GTTTGCCTTG ACCTGCGGCG TGGCCTTCAG TGGCTTTGCC 
ATATCCGGTT ATAATGTCAA TCACCTGGAT ATTGCTCCTC GTTATGCTAG TATATTGATG 
GGTCTTTCGA ATGGAATTGG TACTCTGGCC GGCATCATTG TGCCCTATGC CCTTGATGGC 
CTCATCCAAG CTAATCCTAC CGGGTGTTGG ACTACAGTCT TCACCCTGGC CGCCTGTGTT 
CATTTGGTTG GCTGCACTTT CTATGGTATT TTCGCATCTG GAGAGCTGCA GCCGTGGGCG 
GAACCTCCGG CCGAGGAGCA AAAGGTGTGG GCTCCACCAC CAGGTGCCAT TACCAACACG 
GATCCTAGCC AGGCGGGCAT GTTGGGCGAC TACATGAAGG AAACCTCATT CGGTGCCCCC 
GAGTACACTG AGCAGAGCCA AATGCAGCAG TCGACTGCCA TTAGCTACGG CGCCACAGGA 
CACGTGGCCA ACAATCCCTT CGCCATGGCC AGTGGTGCTC CGCCCATTGC GGAGGAGGAT 
GCCCCACCGA CCTACGGGGA TGTTACCAAT CCTGGGCAGT ATGGCTACAC GCAAGGACAA 
ATGCCGTCCT ACGATCCGCA GGGATACCAG CAGCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259939.1
CDS	1041..2939
Translation	MKGLTAFKEKATGVFGGLKPNMEKFEISQSYHGGHGGYEEMEGGDREGRGPGGGHAYDDDDDRPDSPASFEEIERPPLRKIDKYCKAECPCMPARYTIATMACVGFMIAFGMRCNMSAAKLKGEHNGTVFMNWTVAVESHVDSSFFWGYLVTQIPGGFIASKFPANKIFGLSIVSSATLHLFVPFAMTLMHGHVVICVRVLQGLFEGVTYPACHGIWRFWAPPMERSRLATLAFSGSYAGVVVGLPLSGLLADAVGYQAPFYAYGVFGIIWYMFWIWLCFENPRKHPAISIPELKYIEKSLGESAHPTMPSLKTTPWREMMRSMPVYAIIVANFCRSWNFYLLVLFQSSFLKHKFGFKVEEAGFVGSLPHLIMTTIVPFGGMLADHLRKNGILSTTNVRKLFNCGGFGMEGLFFLFVAHSSTATGAMFALTCGVAFSGFAISGYNVNHLDIAPRYASILMGLSNGIGTLAGIIVPYALDGLIQANPTGCWTTVFTLAACVHLVGCTFYGIFASGELQPWAEPPAEEQKVWAPPPGAITNTDPSQAGMLGDYMKETSFGAPEYTEQSQMQQSTAISYGATGHVANNPFAMASGAPPIAEEDAPPTYGDVTNPGQYGYTQGQMPSYDPQGYQQQ

Target ORF information:

RefSeq Version	NM_001273010.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vesicular glutamate transporter, transcript variant C (VGlut), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273010.1

ORF Insert Sequence:

ATGAAGGGTC TGACGGCGTT TAAGGAGAAG GCAACGGGCG TGTTTGGCGG CCTGAAGCCC 
AATATGGAGA AGTTTGAGAT ATCGCAGAGC TATCATGGTG GCCACGGTGG CTACGAGGAA 
ATGGAGGGGG GCGACCGGGA GGGCCGCGGA CCAGGCGGCG GCCACGCCTA CGACGACGAT 
GACGACCGGC CCGACTCGCC CGCCTCCTTC GAGGAGATCG AGCGCCCGCC GCTCCGCAAG 
ATCGACAAGT ACTGCAAGGC GGAGTGTCCT TGCATGCCGG CCCGCTACAC CATCGCCACC 
ATGGCCTGCG TGGGATTCAT GATCGCCTTC GGCATGAGGT GCAATATGTC GGCGGCCAAG 
CTCAAAGGGG AGCACAATGG GACTGTGTTC ATGAACTGGA CGGTCGCCGT GGAGAGCCAT 
GTGGACTCGT CCTTCTTCTG GGGCTATCTG GTGACGCAGA TTCCCGGCGG CTTCATCGCC 
TCCAAGTTCC CGGCCAACAA GATATTCGGA CTGTCCATCG TGAGCTCCGC CACGCTGCAT 
CTCTTCGTGC CATTCGCCAT GACCCTGATG CACGGTCATG TGGTGATTTG CGTGAGGGTC 
CTGCAAGGAC TCTTCGAGGG CGTTACCTAT CCAGCTTGCC ATGGTATCTG GCGTTTCTGG 
GCGCCGCCCA TGGAGCGCTC CCGACTGGCG ACGCTGGCCT TTTCCGGTTC CTATGCGGGC 
GTGGTGGTTG GACTTCCGCT CTCCGGACTT CTGGCCGATG CCGTGGGCTA CCAGGCGCCG 
TTCTACGCCT ACGGGGTGTT CGGAATCATA TGGTACATGT TCTGGATATG GTTGTGCTTC 
GAGAACCCGC GCAAACATCC GGCCATCAGC ATACCCGAGT TGAAGTACAT CGAGAAATCG 
CTCGGGGAGT CGGCTCATCC CACGATGCCA TCCCTGAAGA CGACTCCGTG GCGGGAAATG 
ATGCGCTCGA TGCCGGTCTA CGCCATCATT GTGGCCAACT TCTGCCGCTC CTGGAACTTC 
TACCTCCTGG TGCTGTTCCA GTCCTCGTTC CTCAAGCACA AGTTCGGTTT TAAGGTGGAG 
GAGGCGGGCT TCGTGGGCTC GCTGCCCCAC TTGATCATGA CTACGATAGT TCCATTTGGC 
GGCATGTTGG CGGATCACCT GCGAAAGAAT GGTATCCTGT CCACCACCAA TGTGCGCAAG 
CTCTTCAATT GCGGCGGCTT TGGCATGGAG GGTCTGTTTT TCCTATTCGT GGCACATTCC 
TCAACGGCGA CGGGTGCCAT GTTTGCCTTG ACCTGCGGCG TGGCCTTCAG TGGCTTTGCC 
ATATCCGGTT ATAATGTCAA TCACCTGGAT ATTGCTCCTC GTTATGCTAG TATATTGATG 
GGTCTTTCGA ATGGAATTGG TACTCTGGCC GGCATCATTG TGCCCTATGC CCTTGATGGC 
CTCATCCAAG CTAATCCTAC CGGGTGTTGG ACTACAGTCT TCACCCTGGC CGCCTGTGTT 
CATTTGGTTG GCTGCACTTT CTATGGTATT TTCGCATCTG GAGAGCTGCA GCCGTGGGCG 
GAACCTCCGG CCGAGGAGCA AAAGGTGTGG GCTCCACCAC CAGGTGCCAT TACCAACACG 
GATCCTAGCC AGGCGGGCAT GTTGGGCGAC TACATGAAGG AAACCTCATT CGGTGCCCCC 
GAGTACACTG AGCAGAGCCA AATGCAGCAG TCGACTGCCA TTAGCTACGG CGCCACAGGA 
CACGTGGCCA ACAATCCCTT CGCCATGGCC AGTGGTGCTC CGCCCATTGC GGAGGAGGAT 
GCCCCACCGA CCTACGGGGA TGTTACCAAT CCTGGGCAGT ATGGCTACAC GCAAGGACAA 
ATGCCGTCCT ACGATCCGCA GGGATACCAG CAGCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VGlut ( NM_001258919.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245848.1 Drosophila melanogaster vesicular glutamate transporter, transcript variant B (VGlut), mRNA.

Price & Availability↑

CloneID	OFa13501
Clone ID Related Accession (Same CDS sequence)	NM_134837.3 , NM_001273010.1 , NM_001258919.1
Accession Version	NM_001258919.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1899bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vesicular glutamate transporter, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGGGTC TGACGGCGTT TAAGGAGAAG GCAACGGGCG TGTTTGGCGG CCTGAAGCCC 
AATATGGAGA AGTTTGAGAT ATCGCAGAGC TATCATGGTG GCCACGGTGG CTACGAGGAA 
ATGGAGGGGG GCGACCGGGA GGGCCGCGGA CCAGGCGGCG GCCACGCCTA CGACGACGAT 
GACGACCGGC CCGACTCGCC CGCCTCCTTC GAGGAGATCG AGCGCCCGCC GCTCCGCAAG 
ATCGACAAGT ACTGCAAGGC GGAGTGTCCT TGCATGCCGG CCCGCTACAC CATCGCCACC 
ATGGCCTGCG TGGGATTCAT GATCGCCTTC GGCATGAGGT GCAATATGTC GGCGGCCAAG 
CTCAAAGGGG AGCACAATGG GACTGTGTTC ATGAACTGGA CGGTCGCCGT GGAGAGCCAT 
GTGGACTCGT CCTTCTTCTG GGGCTATCTG GTGACGCAGA TTCCCGGCGG CTTCATCGCC 
TCCAAGTTCC CGGCCAACAA GATATTCGGA CTGTCCATCG TGAGCTCCGC CACGCTGCAT 
CTCTTCGTGC CATTCGCCAT GACCCTGATG CACGGTCATG TGGTGATTTG CGTGAGGGTC 
CTGCAAGGAC TCTTCGAGGG CGTTACCTAT CCAGCTTGCC ATGGTATCTG GCGTTTCTGG 
GCGCCGCCCA TGGAGCGCTC CCGACTGGCG ACGCTGGCCT TTTCCGGTTC CTATGCGGGC 
GTGGTGGTTG GACTTCCGCT CTCCGGACTT CTGGCCGATG CCGTGGGCTA CCAGGCGCCG 
TTCTACGCCT ACGGGGTGTT CGGAATCATA TGGTACATGT TCTGGATATG GTTGTGCTTC 
GAGAACCCGC GCAAACATCC GGCCATCAGC ATACCCGAGT TGAAGTACAT CGAGAAATCG 
CTCGGGGAGT CGGCTCATCC CACGATGCCA TCCCTGAAGA CGACTCCGTG GCGGGAAATG 
ATGCGCTCGA TGCCGGTCTA CGCCATCATT GTGGCCAACT TCTGCCGCTC CTGGAACTTC 
TACCTCCTGG TGCTGTTCCA GTCCTCGTTC CTCAAGCACA AGTTCGGTTT TAAGGTGGAG 
GAGGCGGGCT TCGTGGGCTC GCTGCCCCAC TTGATCATGA CTACGATAGT TCCATTTGGC 
GGCATGTTGG CGGATCACCT GCGAAAGAAT GGTATCCTGT CCACCACCAA TGTGCGCAAG 
CTCTTCAATT GCGGCGGCTT TGGCATGGAG GGTCTGTTTT TCCTATTCGT GGCACATTCC 
TCAACGGCGA CGGGTGCCAT GTTTGCCTTG ACCTGCGGCG TGGCCTTCAG TGGCTTTGCC 
ATATCCGGTT ATAATGTCAA TCACCTGGAT ATTGCTCCTC GTTATGCTAG TATATTGATG 
GGTCTTTCGA ATGGAATTGG TACTCTGGCC GGCATCATTG TGCCCTATGC CCTTGATGGC 
CTCATCCAAG CTAATCCTAC CGGGTGTTGG ACTACAGTCT TCACCCTGGC CGCCTGTGTT 
CATTTGGTTG GCTGCACTTT CTATGGTATT TTCGCATCTG GAGAGCTGCA GCCGTGGGCG 
GAACCTCCGG CCGAGGAGCA AAAGGTGTGG GCTCCACCAC CAGGTGCCAT TACCAACACG 
GATCCTAGCC AGGCGGGCAT GTTGGGCGAC TACATGAAGG AAACCTCATT CGGTGCCCCC 
GAGTACACTG AGCAGAGCCA AATGCAGCAG TCGACTGCCA TTAGCTACGG CGCCACAGGA 
CACGTGGCCA ACAATCCCTT CGCCATGGCC AGTGGTGCTC CGCCCATTGC GGAGGAGGAT 
GCCCCACCGA CCTACGGGGA TGTTACCAAT CCTGGGCAGT ATGGCTACAC GCAAGGACAA 
ATGCCGTCCT ACGATCCGCA GGGATACCAG CAGCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245848.1
CDS	798..2696
Translation	MKGLTAFKEKATGVFGGLKPNMEKFEISQSYHGGHGGYEEMEGGDREGRGPGGGHAYDDDDDRPDSPASFEEIERPPLRKIDKYCKAECPCMPARYTIATMACVGFMIAFGMRCNMSAAKLKGEHNGTVFMNWTVAVESHVDSSFFWGYLVTQIPGGFIASKFPANKIFGLSIVSSATLHLFVPFAMTLMHGHVVICVRVLQGLFEGVTYPACHGIWRFWAPPMERSRLATLAFSGSYAGVVVGLPLSGLLADAVGYQAPFYAYGVFGIIWYMFWIWLCFENPRKHPAISIPELKYIEKSLGESAHPTMPSLKTTPWREMMRSMPVYAIIVANFCRSWNFYLLVLFQSSFLKHKFGFKVEEAGFVGSLPHLIMTTIVPFGGMLADHLRKNGILSTTNVRKLFNCGGFGMEGLFFLFVAHSSTATGAMFALTCGVAFSGFAISGYNVNHLDIAPRYASILMGLSNGIGTLAGIIVPYALDGLIQANPTGCWTTVFTLAACVHLVGCTFYGIFASGELQPWAEPPAEEQKVWAPPPGAITNTDPSQAGMLGDYMKETSFGAPEYTEQSQMQQSTAISYGATGHVANNPFAMASGAPPIAEEDAPPTYGDVTNPGQYGYTQGQMPSYDPQGYQQQ

Target ORF information:

RefSeq Version	NM_001258919.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vesicular glutamate transporter, transcript variant B (VGlut), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258919.1

ORF Insert Sequence:

ATGAAGGGTC TGACGGCGTT TAAGGAGAAG GCAACGGGCG TGTTTGGCGG CCTGAAGCCC 
AATATGGAGA AGTTTGAGAT ATCGCAGAGC TATCATGGTG GCCACGGTGG CTACGAGGAA 
ATGGAGGGGG GCGACCGGGA GGGCCGCGGA CCAGGCGGCG GCCACGCCTA CGACGACGAT 
GACGACCGGC CCGACTCGCC CGCCTCCTTC GAGGAGATCG AGCGCCCGCC GCTCCGCAAG 
ATCGACAAGT ACTGCAAGGC GGAGTGTCCT TGCATGCCGG CCCGCTACAC CATCGCCACC 
ATGGCCTGCG TGGGATTCAT GATCGCCTTC GGCATGAGGT GCAATATGTC GGCGGCCAAG 
CTCAAAGGGG AGCACAATGG GACTGTGTTC ATGAACTGGA CGGTCGCCGT GGAGAGCCAT 
GTGGACTCGT CCTTCTTCTG GGGCTATCTG GTGACGCAGA TTCCCGGCGG CTTCATCGCC 
TCCAAGTTCC CGGCCAACAA GATATTCGGA CTGTCCATCG TGAGCTCCGC CACGCTGCAT 
CTCTTCGTGC CATTCGCCAT GACCCTGATG CACGGTCATG TGGTGATTTG CGTGAGGGTC 
CTGCAAGGAC TCTTCGAGGG CGTTACCTAT CCAGCTTGCC ATGGTATCTG GCGTTTCTGG 
GCGCCGCCCA TGGAGCGCTC CCGACTGGCG ACGCTGGCCT TTTCCGGTTC CTATGCGGGC 
GTGGTGGTTG GACTTCCGCT CTCCGGACTT CTGGCCGATG CCGTGGGCTA CCAGGCGCCG 
TTCTACGCCT ACGGGGTGTT CGGAATCATA TGGTACATGT TCTGGATATG GTTGTGCTTC 
GAGAACCCGC GCAAACATCC GGCCATCAGC ATACCCGAGT TGAAGTACAT CGAGAAATCG 
CTCGGGGAGT CGGCTCATCC CACGATGCCA TCCCTGAAGA CGACTCCGTG GCGGGAAATG 
ATGCGCTCGA TGCCGGTCTA CGCCATCATT GTGGCCAACT TCTGCCGCTC CTGGAACTTC 
TACCTCCTGG TGCTGTTCCA GTCCTCGTTC CTCAAGCACA AGTTCGGTTT TAAGGTGGAG 
GAGGCGGGCT TCGTGGGCTC GCTGCCCCAC TTGATCATGA CTACGATAGT TCCATTTGGC 
GGCATGTTGG CGGATCACCT GCGAAAGAAT GGTATCCTGT CCACCACCAA TGTGCGCAAG 
CTCTTCAATT GCGGCGGCTT TGGCATGGAG GGTCTGTTTT TCCTATTCGT GGCACATTCC 
TCAACGGCGA CGGGTGCCAT GTTTGCCTTG ACCTGCGGCG TGGCCTTCAG TGGCTTTGCC 
ATATCCGGTT ATAATGTCAA TCACCTGGAT ATTGCTCCTC GTTATGCTAG TATATTGATG 
GGTCTTTCGA ATGGAATTGG TACTCTGGCC GGCATCATTG TGCCCTATGC CCTTGATGGC 
CTCATCCAAG CTAATCCTAC CGGGTGTTGG ACTACAGTCT TCACCCTGGC CGCCTGTGTT 
CATTTGGTTG GCTGCACTTT CTATGGTATT TTCGCATCTG GAGAGCTGCA GCCGTGGGCG 
GAACCTCCGG CCGAGGAGCA AAAGGTGTGG GCTCCACCAC CAGGTGCCAT TACCAACACG 
GATCCTAGCC AGGCGGGCAT GTTGGGCGAC TACATGAAGG AAACCTCATT CGGTGCCCCC 
GAGTACACTG AGCAGAGCCA AATGCAGCAG TCGACTGCCA TTAGCTACGG CGCCACAGGA 
CACGTGGCCA ACAATCCCTT CGCCATGGCC AGTGGTGCTC CGCCCATTGC GGAGGAGGAT 
GCCCCACCGA CCTACGGGGA TGTTACCAAT CCTGGGCAGT ATGGCTACAC GCAAGGACAA 
ATGCCGTCCT ACGATCCGCA GGGATACCAG CAGCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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VGlut cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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