Eaat2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Eaat2
Entrez Gene ID	33247
Full Name	Excitatory amino acid transporter 2
Synonyms	CG3159,Dmel\CG3159,EAAT,dEAAT2,dEaat2
General protein information	Preferred Names Excitatory amino acid transporter 2 Names CG3159 gene product from transcript CG3159-RA CG3159-PA CG3159-PC CG3159-PD Eaat2-PA Eaat2-PC Eaat2-PD excitatory amino acid transporter 2 sodium-dependent excitatory amino acid transporter 2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 21E2-21E2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001169374.2	NP_001162845.1	excitatory amino acid transporter 2, isoform D
NM_001169373.2	NP_001162844.1	excitatory amino acid transporter 2, isoform C
NM_058079.3	NP_477427.1	excitatory amino acid transporter 2, isoform A

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007903	XP_555593.3
Drosophila melanogaster (fruit fly)	Eaat2	NP_477427.1
Danio rerio (zebrafish)	slc1a9	NP_001177688.1
Xenopus tropicalis (tropical clawed frog)	LOC100496635	XP_002938568.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that EAAT2 functions as a taurine transporter in vivo and is physiologically required for the sensory perception of specific environmental molecules.
Title: Involvement of the Drosophila taurine/aspartate transporter dEAAT2 in selective olfactory and gustatory perceptions.

Taurine and aspartate are transported with similar K(m) and relative efficacy and behave as mutually competitive inhibitors; dEAAT2 is actually an aspartate/taurine transporter
Title: High affinity transport of taurine by the Drosophila aspartate transporter dEAAT2.

Terminal glial differentiation involves regulated expression of the excitatory amino acid transporters in the Drosophila embryonic CNS.
Title: Terminal glial differentiation involves regulated expression of the excitatory amino acid transporters in the Drosophila embryonic CNS.

The following Eaat2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Eaat2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa13380	NM_001169374.2 Latest version!	Drosophila melanogaster excitatory amino acid transporter 2, transcript variant D (Eaat2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa13380	NM_058079.3 Latest version!	Drosophila melanogaster excitatory amino acid transporter 2, transcript variant A (Eaat2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa15874	NM_001169373.2 Latest version!	Drosophila melanogaster excitatory amino acid transporter 2, transcript variant C (Eaat2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Eaat2 ( NM_001169374.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001162845.1 Drosophila melanogaster excitatory amino acid transporter 2, transcript variant D (Eaat2), mRNA.

Price & Availability↑

CloneID	OFa13380
Clone ID Related Accession (Same CDS sequence)	NM_001169374.2 , NM_058079.3
Accession Version	NM_001169374.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1692bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	excitatory amino acid transporter 2, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001169374.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTCCCC CCACCTCAAC TGAGCTGCCT CCGAAGACCA CGGAATGCAG CGATGCCCCC 
GTGGAGTTGA CCGGTTACCG CAGGTGGCTC TCCGAGAATC TAATGCTCCT GGTCACCCTT 
TCCGGAGTTC TCCTTGGTGT AATACTAGGA CTCTCCTTAC GGCCGCTTAA TTTACATGGG 
GACTCCATCA TGCTGATTTC CTATCCTGGC GAACTGTTTA TGCGCGTACT CAAGCTGATG 
ATTTTGCCGC TAGTTATATC GAGCCTTATT GCCGGATCGG CCAGCCTTAA CGCCAAAATG 
AACGGCAAGA TAGCGCTGCG CACGTTGGTT TACTTTGCCA GCACTTCGTT CTTTAACGCT 
GCACTGGGAA TCGCTCTCGT TCTGCTCATC CATCCTGGAA ATCCGGATCT GCACAATGCG 
GACGATCGAT CTACAGATCG GCGGGCAGTT AACCTTCTAG ATAGTCTTTT GGATTTGGGC 
AGGAACGTAT TCCCGGACAA CCTGTTTCAG GCCTCCATTC AGCAGGCGCA CACCGTCTAC 
TTGCCCAAGC CGAGTATCCT GCACGTCTTC AACGAAACGA TGAATGACAC CTTGGCATCC 
GGATCGGAGG CCCAACGCTT GTCTGAAGAC CTTACGGAGG ATGTGGTGCT GGTGAGGGAC 
ATCCAGTACC GCAGTGGAAC CAACACCCTG GGCATCGTCT TCTTCTGCCT GGTGTTTGGT 
ACTTTTTTGG GAACCATCGG CCAGAAGGGT CAGGTCGTGG TTGACTTCTT TGCCGCCATT 
TTTGAGGTGA TCATGAAGAT GGTCACCTGC GTGATGTGGC TGACACCCGT GGGCATTAGT 
TCTGTGATCG CTGGGAAGAT ACTGAGCGTG GGCGATTTGG GCCTGGTGAT GTCGCAACTC 
ATGTGGTTCA TCGTCACGGT GGCCATCGGC GTCTTTATCT ACCAGTTTGT GGTGATGCAG 
GCCATATATT TCGTCGTCGT GCGTCGAAAT CCGTTCAAGT TCTACGCCGG ACTCATCCAG 
GCCATGCTTA CCGCCTTCGC CACGGCCTCA ACCGCCGCTG CCCTGCCCAT TACATTCCGC 
TGCATGAACG AGAAACTCAA GGTGGATCCG CGGATCACGC GATTTGTCCT TCCAATCGGA 
TGCAACATCA ACATGGACGG AACGGCCCTA TATATCGCAG TGGCATCGAT ATTCATTGCC 
CAGATGAGCG GCATGGTTCT GGGATTTGGA GAGCTCCTTA CGGTCCTTCT GACCTCCACG 
GCTGCCTCCA TGAGCTCGGC CAGCGTTCCG AGTGCTGCCC TGGTACTCCT TCTCGTGGTG 
CTGACCGCCA TTGATGCGCC TGTTCAGGAT GTCACGCTGC TCTTCGCCGT GGACTGGTTT 
GTGGATCGGA TTCGCACCAC CAATAATATG CTGGGTGACT GCTACACTGC TGCCGTAGTC 
GAGGAGTTGT CGCGAAAGGA GTTGATGGCT TTGGATGCCG CTTCCGTTAA CTATCAGGAC 
ATGCCCGCTG GCACCCCCAA TGGACATGGA CATGGACATG GACACCACGA TGGAGGACTT 
CTCGAGGGGC AGACCGAGTT GGAGACGAGC AGCAAGTGCG TTATGACAAT GACGGACTCG 
GTGGTGGTGG ACATTAGTGC GGTGATGAAT AACGTGAACC TGCAGCAGGA GCATTGTAAC 
CGCAGGGTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162845.1
CDS	616..2307
Translation	MGPPTSTELPPKTTECSDAPVELTGYRRWLSENLMLLVTLSGVLLGVILGLSLRPLNLHGDSIMLISYPGELFMRVLKLMILPLVISSLIAGSASLNAKMNGKIALRTLVYFASTSFFNAALGIALVLLIHPGNPDLHNADDRSTDRRAVNLLDSLLDLGRNVFPDNLFQASIQQAHTVYLPKPSILHVFNETMNDTLASGSEAQRLSEDLTEDVVLVRDIQYRSGTNTLGIVFFCLVFGTFLGTIGQKGQVVVDFFAAIFEVIMKMVTCVMWLTPVGISSVIAGKILSVGDLGLVMSQLMWFIVTVAIGVFIYQFVVMQAIYFVVVRRNPFKFYAGLIQAMLTAFATASTAAALPITFRCMNEKLKVDPRITRFVLPIGCNINMDGTALYIAVASIFIAQMSGMVLGFGELLTVLLTSTAASMSSASVPSAALVLLLVVLTAIDAPVQDVTLLFAVDWFVDRIRTTNNMLGDCYTAAVVEELSRKELMALDAASVNYQDMPAGTPNGHGHGHGHHDGGLLEGQTELETSSKCVMTMTDSVVVDISAVMNNVNLQQEHCNRRV

Target ORF information:

RefSeq Version	NM_001169374.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster excitatory amino acid transporter 2, transcript variant D (Eaat2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169374.2

ORF Insert Sequence:

ATGGGTCCCC CCACCTCAAC TGAGCTGCCT CCGAAGACCA CGGAATGCAG CGATGCCCCC 
GTGGAGTTGA CCGGTTACCG CAGGTGGCTC TCCGAGAATC TAATGCTCCT GGTCACCCTT 
TCCGGAGTTC TCCTTGGTGT AATACTAGGA CTCTCCTTAC GGCCGCTTAA TTTACATGGG 
GACTCCATCA TGCTGATTTC CTATCCTGGC GAACTGTTTA TGCGCGTACT CAAGCTGATG 
ATTTTGCCGC TAGTTATATC GAGCCTTATT GCCGGATCGG CCAGCCTTAA CGCCAAAATG 
AACGGCAAGA TAGCGCTGCG CACGTTGGTT TACTTTGCCA GCACTTCGTT CTTTAACGCT 
GCACTGGGAA TCGCTCTCGT TCTGCTCATC CATCCTGGAA ATCCGGATCT GCACAATGCG 
GACGATCGAT CTACAGATCG GCGGGCAGTT AACCTTCTAG ATAGTCTTTT GGATTTGGGC 
AGGAACGTAT TCCCGGACAA CCTGTTTCAG GCCTCCATTC AGCAGGCGCA CACCGTCTAC 
TTGCCCAAGC CGAGTATCCT GCACGTCTTC AACGAAACGA TGAATGACAC CTTGGCATCC 
GGATCGGAGG CCCAACGCTT GTCTGAAGAC CTTACGGAGG ATGTGGTGCT GGTGAGGGAC 
ATCCAGTACC GCAGTGGAAC CAACACCCTG GGCATCGTCT TCTTCTGCCT GGTGTTTGGT 
ACTTTTTTGG GAACCATCGG CCAGAAGGGT CAGGTCGTGG TTGACTTCTT TGCCGCCATT 
TTTGAGGTGA TCATGAAGAT GGTCACCTGC GTGATGTGGC TGACACCCGT GGGCATTAGT 
TCTGTGATCG CTGGGAAGAT ACTGAGCGTG GGCGATTTGG GCCTGGTGAT GTCGCAACTC 
ATGTGGTTCA TCGTCACGGT GGCCATCGGC GTCTTTATCT ACCAGTTTGT GGTGATGCAG 
GCCATATATT TCGTCGTCGT GCGTCGAAAT CCGTTCAAGT TCTACGCCGG ACTCATCCAG 
GCCATGCTTA CCGCCTTCGC CACGGCCTCA ACCGCCGCTG CCCTGCCCAT TACATTCCGC 
TGCATGAACG AGAAACTCAA GGTGGATCCG CGGATCACGC GATTTGTCCT TCCAATCGGA 
TGCAACATCA ACATGGACGG AACGGCCCTA TATATCGCAG TGGCATCGAT ATTCATTGCC 
CAGATGAGCG GCATGGTTCT GGGATTTGGA GAGCTCCTTA CGGTCCTTCT GACCTCCACG 
GCTGCCTCCA TGAGCTCGGC CAGCGTTCCG AGTGCTGCCC TGGTACTCCT TCTCGTGGTG 
CTGACCGCCA TTGATGCGCC TGTTCAGGAT GTCACGCTGC TCTTCGCCGT GGACTGGTTT 
GTGGATCGGA TTCGCACCAC CAATAATATG CTGGGTGACT GCTACACTGC TGCCGTAGTC 
GAGGAGTTGT CGCGAAAGGA GTTGATGGCT TTGGATGCCG CTTCCGTTAA CTATCAGGAC 
ATGCCCGCTG GCACCCCCAA TGGACATGGA CATGGACATG GACACCACGA TGGAGGACTT 
CTCGAGGGGC AGACCGAGTT GGAGACGAGC AGCAAGTGCG TTATGACAAT GACGGACTCG 
GTGGTGGTGG ACATTAGTGC GGTGATGAAT AACGTGAACC TGCAGCAGGA GCATTGTAAC 
CGCAGGGTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eaat2 ( NM_058079.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477427.1 Drosophila melanogaster excitatory amino acid transporter 2, transcript variant A (Eaat2), mRNA.

Price & Availability↑

CloneID	OFa13380
Clone ID Related Accession (Same CDS sequence)	NM_001169374.2 , NM_058079.3
Accession Version	NM_058079.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1692bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	excitatory amino acid transporter 2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 26, 2009 this sequence version replaced NM_058079.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTCCCC CCACCTCAAC TGAGCTGCCT CCGAAGACCA CGGAATGCAG CGATGCCCCC 
GTGGAGTTGA CCGGTTACCG CAGGTGGCTC TCCGAGAATC TAATGCTCCT GGTCACCCTT 
TCCGGAGTTC TCCTTGGTGT AATACTAGGA CTCTCCTTAC GGCCGCTTAA TTTACATGGG 
GACTCCATCA TGCTGATTTC CTATCCTGGC GAACTGTTTA TGCGCGTACT CAAGCTGATG 
ATTTTGCCGC TAGTTATATC GAGCCTTATT GCCGGATCGG CCAGCCTTAA CGCCAAAATG 
AACGGCAAGA TAGCGCTGCG CACGTTGGTT TACTTTGCCA GCACTTCGTT CTTTAACGCT 
GCACTGGGAA TCGCTCTCGT TCTGCTCATC CATCCTGGAA ATCCGGATCT GCACAATGCG 
GACGATCGAT CTACAGATCG GCGGGCAGTT AACCTTCTAG ATAGTCTTTT GGATTTGGGC 
AGGAACGTAT TCCCGGACAA CCTGTTTCAG GCCTCCATTC AGCAGGCGCA CACCGTCTAC 
TTGCCCAAGC CGAGTATCCT GCACGTCTTC AACGAAACGA TGAATGACAC CTTGGCATCC 
GGATCGGAGG CCCAACGCTT GTCTGAAGAC CTTACGGAGG ATGTGGTGCT GGTGAGGGAC 
ATCCAGTACC GCAGTGGAAC CAACACCCTG GGCATCGTCT TCTTCTGCCT GGTGTTTGGT 
ACTTTTTTGG GAACCATCGG CCAGAAGGGT CAGGTCGTGG TTGACTTCTT TGCCGCCATT 
TTTGAGGTGA TCATGAAGAT GGTCACCTGC GTGATGTGGC TGACACCCGT GGGCATTAGT 
TCTGTGATCG CTGGGAAGAT ACTGAGCGTG GGCGATTTGG GCCTGGTGAT GTCGCAACTC 
ATGTGGTTCA TCGTCACGGT GGCCATCGGC GTCTTTATCT ACCAGTTTGT GGTGATGCAG 
GCCATATATT TCGTCGTCGT GCGTCGAAAT CCGTTCAAGT TCTACGCCGG ACTCATCCAG 
GCCATGCTTA CCGCCTTCGC CACGGCCTCA ACCGCCGCTG CCCTGCCCAT TACATTCCGC 
TGCATGAACG AGAAACTCAA GGTGGATCCG CGGATCACGC GATTTGTCCT TCCAATCGGA 
TGCAACATCA ACATGGACGG AACGGCCCTA TATATCGCAG TGGCATCGAT ATTCATTGCC 
CAGATGAGCG GCATGGTTCT GGGATTTGGA GAGCTCCTTA CGGTCCTTCT GACCTCCACG 
GCTGCCTCCA TGAGCTCGGC CAGCGTTCCG AGTGCTGCCC TGGTACTCCT TCTCGTGGTG 
CTGACCGCCA TTGATGCGCC TGTTCAGGAT GTCACGCTGC TCTTCGCCGT GGACTGGTTT 
GTGGATCGGA TTCGCACCAC CAATAATATG CTGGGTGACT GCTACACTGC TGCCGTAGTC 
GAGGAGTTGT CGCGAAAGGA GTTGATGGCT TTGGATGCCG CTTCCGTTAA CTATCAGGAC 
ATGCCCGCTG GCACCCCCAA TGGACATGGA CATGGACATG GACACCACGA TGGAGGACTT 
CTCGAGGGGC AGACCGAGTT GGAGACGAGC AGCAAGTGCG TTATGACAAT GACGGACTCG 
GTGGTGGTGG ACATTAGTGC GGTGATGAAT AACGTGAACC TGCAGCAGGA GCATTGTAAC 
CGCAGGGTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477427.1
CDS	504..2195
Translation	MGPPTSTELPPKTTECSDAPVELTGYRRWLSENLMLLVTLSGVLLGVILGLSLRPLNLHGDSIMLISYPGELFMRVLKLMILPLVISSLIAGSASLNAKMNGKIALRTLVYFASTSFFNAALGIALVLLIHPGNPDLHNADDRSTDRRAVNLLDSLLDLGRNVFPDNLFQASIQQAHTVYLPKPSILHVFNETMNDTLASGSEAQRLSEDLTEDVVLVRDIQYRSGTNTLGIVFFCLVFGTFLGTIGQKGQVVVDFFAAIFEVIMKMVTCVMWLTPVGISSVIAGKILSVGDLGLVMSQLMWFIVTVAIGVFIYQFVVMQAIYFVVVRRNPFKFYAGLIQAMLTAFATASTAAALPITFRCMNEKLKVDPRITRFVLPIGCNINMDGTALYIAVASIFIAQMSGMVLGFGELLTVLLTSTAASMSSASVPSAALVLLLVVLTAIDAPVQDVTLLFAVDWFVDRIRTTNNMLGDCYTAAVVEELSRKELMALDAASVNYQDMPAGTPNGHGHGHGHHDGGLLEGQTELETSSKCVMTMTDSVVVDISAVMNNVNLQQEHCNRRV

Target ORF information:

RefSeq Version	NM_058079.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster excitatory amino acid transporter 2, transcript variant A (Eaat2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058079.3

ORF Insert Sequence:

ATGGGTCCCC CCACCTCAAC TGAGCTGCCT CCGAAGACCA CGGAATGCAG CGATGCCCCC 
GTGGAGTTGA CCGGTTACCG CAGGTGGCTC TCCGAGAATC TAATGCTCCT GGTCACCCTT 
TCCGGAGTTC TCCTTGGTGT AATACTAGGA CTCTCCTTAC GGCCGCTTAA TTTACATGGG 
GACTCCATCA TGCTGATTTC CTATCCTGGC GAACTGTTTA TGCGCGTACT CAAGCTGATG 
ATTTTGCCGC TAGTTATATC GAGCCTTATT GCCGGATCGG CCAGCCTTAA CGCCAAAATG 
AACGGCAAGA TAGCGCTGCG CACGTTGGTT TACTTTGCCA GCACTTCGTT CTTTAACGCT 
GCACTGGGAA TCGCTCTCGT TCTGCTCATC CATCCTGGAA ATCCGGATCT GCACAATGCG 
GACGATCGAT CTACAGATCG GCGGGCAGTT AACCTTCTAG ATAGTCTTTT GGATTTGGGC 
AGGAACGTAT TCCCGGACAA CCTGTTTCAG GCCTCCATTC AGCAGGCGCA CACCGTCTAC 
TTGCCCAAGC CGAGTATCCT GCACGTCTTC AACGAAACGA TGAATGACAC CTTGGCATCC 
GGATCGGAGG CCCAACGCTT GTCTGAAGAC CTTACGGAGG ATGTGGTGCT GGTGAGGGAC 
ATCCAGTACC GCAGTGGAAC CAACACCCTG GGCATCGTCT TCTTCTGCCT GGTGTTTGGT 
ACTTTTTTGG GAACCATCGG CCAGAAGGGT CAGGTCGTGG TTGACTTCTT TGCCGCCATT 
TTTGAGGTGA TCATGAAGAT GGTCACCTGC GTGATGTGGC TGACACCCGT GGGCATTAGT 
TCTGTGATCG CTGGGAAGAT ACTGAGCGTG GGCGATTTGG GCCTGGTGAT GTCGCAACTC 
ATGTGGTTCA TCGTCACGGT GGCCATCGGC GTCTTTATCT ACCAGTTTGT GGTGATGCAG 
GCCATATATT TCGTCGTCGT GCGTCGAAAT CCGTTCAAGT TCTACGCCGG ACTCATCCAG 
GCCATGCTTA CCGCCTTCGC CACGGCCTCA ACCGCCGCTG CCCTGCCCAT TACATTCCGC 
TGCATGAACG AGAAACTCAA GGTGGATCCG CGGATCACGC GATTTGTCCT TCCAATCGGA 
TGCAACATCA ACATGGACGG AACGGCCCTA TATATCGCAG TGGCATCGAT ATTCATTGCC 
CAGATGAGCG GCATGGTTCT GGGATTTGGA GAGCTCCTTA CGGTCCTTCT GACCTCCACG 
GCTGCCTCCA TGAGCTCGGC CAGCGTTCCG AGTGCTGCCC TGGTACTCCT TCTCGTGGTG 
CTGACCGCCA TTGATGCGCC TGTTCAGGAT GTCACGCTGC TCTTCGCCGT GGACTGGTTT 
GTGGATCGGA TTCGCACCAC CAATAATATG CTGGGTGACT GCTACACTGC TGCCGTAGTC 
GAGGAGTTGT CGCGAAAGGA GTTGATGGCT TTGGATGCCG CTTCCGTTAA CTATCAGGAC 
ATGCCCGCTG GCACCCCCAA TGGACATGGA CATGGACATG GACACCACGA TGGAGGACTT 
CTCGAGGGGC AGACCGAGTT GGAGACGAGC AGCAAGTGCG TTATGACAAT GACGGACTCG 
GTGGTGGTGG ACATTAGTGC GGTGATGAAT AACGTGAACC TGCAGCAGGA GCATTGTAAC 
CGCAGGGTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eaat2 ( NM_001169373.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001162844.1 Drosophila melanogaster excitatory amino acid transporter 2, transcript variant C (Eaat2), mRNA.

Price & Availability↑

CloneID	OFa15874
Clone ID Related Accession (Same CDS sequence)	NM_001169373.2
Accession Version	NM_001169373.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	excitatory amino acid transporter 2, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001169373.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAATGG AAAAGTCTGG CAAATTTTCA GATTACCTCT TCGCCAAAAT GGGTCCCCCC 
ACCTCAACTG AGCTGCCTCC GAAGACCACG GAATGCAGCG ATGCCCCCGT GGAGTTGACC 
GGTTACCGCA GGTGGCTCTC CGAGAATCTA ATGCTCCTGG TCACCCTTTC CGGAGTTCTC 
CTTGGTGTAA TACTAGGACT CTCCTTACGG CCGCTTAATT TACATGGGGA CTCCATCATG 
CTGATTTCCT ATCCTGGCGA ACTGTTTATG CGCGTACTCA AGCTGATGAT TTTGCCGCTA 
GTTATATCGA GCCTTATTGC CGGATCGGCC AGCCTTAACG CCAAAATGAA CGGCAAGATA 
GCGCTGCGCA CGTTGGTTTA CTTTGCCAGC ACTTCGTTCT TTAACGCTGC ACTGGGAATC 
GCTCTCGTTC TGCTCATCCA TCCTGGAAAT CCGGATCTGC ACAATGCGGA CGATCGATCT 
ACAGATCGGC GGGCAGTTAA CCTTCTAGAT AGTCTTTTGG ATTTGGGCAG GAACGTATTC 
CCGGACAACC TGTTTCAGGC CTCCATTCAG CAGGCGCACA CCGTCTACTT GCCCAAGCCG 
AGTATCCTGC ACGTCTTCAA CGAAACGATG AATGACACCT TGGCATCCGG ATCGGAGGCC 
CAACGCTTGT CTGAAGACCT TACGGAGGAT GTGGTGCTGG TGAGGGACAT CCAGTACCGC 
AGTGGAACCA ACACCCTGGG CATCGTCTTC TTCTGCCTGG TGTTTGGTAC TTTTTTGGGA 
ACCATCGGCC AGAAGGGTCA GGTCGTGGTT GACTTCTTTG CCGCCATTTT TGAGGTGATC 
ATGAAGATGG TCACCTGCGT GATGTGGCTG ACACCCGTGG GCATTAGTTC TGTGATCGCT 
GGGAAGATAC TGAGCGTGGG CGATTTGGGC CTGGTGATGT CGCAACTCAT GTGGTTCATC 
GTCACGGTGG CCATCGGCGT CTTTATCTAC CAGTTTGTGG TGATGCAGGC CATATATTTC 
GTCGTCGTGC GTCGAAATCC GTTCAAGTTC TACGCCGGAC TCATCCAGGC CATGCTTACC 
GCCTTCGCCA CGGCCTCAAC CGCCGCTGCC CTGCCCATTA CATTCCGCTG CATGAACGAG 
AAACTCAAGG TGGATCCGCG GATCACGCGA TTTGTCCTTC CAATCGGATG CAACATCAAC 
ATGGACGGAA CGGCCCTATA TATCGCAGTG GCATCGATAT TCATTGCCCA GATGAGCGGC 
ATGGTTCTGG GATTTGGAGA GCTCCTTACG GTCCTTCTGA CCTCCACGGC TGCCTCCATG 
AGCTCGGCCA GCGTTCCGAG TGCTGCCCTG GTACTCCTTC TCGTGGTGCT GACCGCCATT 
GATGCGCCTG TTCAGGATGT CACGCTGCTC TTCGCCGTGG ACTGGTTTGT GGATCGGATT 
CGCACCACCA ATAATATGCT GGGTGACTGC TACACTGCTG CCGTAGTCGA GGAGTTGTCG 
CGAAAGGAGT TGATGGCTTT GGATGCCGCT TCCGTTAACT ATCAGGACAT GCCCGCTGGC 
ACCCCCAATG GACATGGACA TGGACATGGA CACCACGATG GAGGACTTCT CGAGGGGCAG 
ACCGAGTTGG AGACGAGCAG CAAGTGCGTT ATGACAATGA CGGACTCGGT GGTGGTGGAC 
ATTAGTGCGG TGATGAATAA CGTGAACCTG CAGCAGGAGC ATTGTAACCG CAGGGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162844.1
CDS	797..2536
Translation	MEMEKSGKFSDYLFAKMGPPTSTELPPKTTECSDAPVELTGYRRWLSENLMLLVTLSGVLLGVILGLSLRPLNLHGDSIMLISYPGELFMRVLKLMILPLVISSLIAGSASLNAKMNGKIALRTLVYFASTSFFNAALGIALVLLIHPGNPDLHNADDRSTDRRAVNLLDSLLDLGRNVFPDNLFQASIQQAHTVYLPKPSILHVFNETMNDTLASGSEAQRLSEDLTEDVVLVRDIQYRSGTNTLGIVFFCLVFGTFLGTIGQKGQVVVDFFAAIFEVIMKMVTCVMWLTPVGISSVIAGKILSVGDLGLVMSQLMWFIVTVAIGVFIYQFVVMQAIYFVVVRRNPFKFYAGLIQAMLTAFATASTAAALPITFRCMNEKLKVDPRITRFVLPIGCNINMDGTALYIAVASIFIAQMSGMVLGFGELLTVLLTSTAASMSSASVPSAALVLLLVVLTAIDAPVQDVTLLFAVDWFVDRIRTTNNMLGDCYTAAVVEELSRKELMALDAASVNYQDMPAGTPNGHGHGHGHHDGGLLEGQTELETSSKCVMTMTDSVVVDISAVMNNVNLQQEHCNRRV

Target ORF information:

RefSeq Version	NM_001169373.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster excitatory amino acid transporter 2, transcript variant C (Eaat2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169373.2

ORF Insert Sequence:

ATGGAAATGG AAAAGTCTGG CAAATTTTCA GATTACCTCT TCGCCAAAAT GGGTCCCCCC 
ACCTCAACTG AGCTGCCTCC GAAGACCACG GAATGCAGCG ATGCCCCCGT GGAGTTGACC 
GGTTACCGCA GGTGGCTCTC CGAGAATCTA ATGCTCCTGG TCACCCTTTC CGGAGTTCTC 
CTTGGTGTAA TACTAGGACT CTCCTTACGG CCGCTTAATT TACATGGGGA CTCCATCATG 
CTGATTTCCT ATCCTGGCGA ACTGTTTATG CGCGTACTCA AGCTGATGAT TTTGCCGCTA 
GTTATATCGA GCCTTATTGC CGGATCGGCC AGCCTTAACG CCAAAATGAA CGGCAAGATA 
GCGCTGCGCA CGTTGGTTTA CTTTGCCAGC ACTTCGTTCT TTAACGCTGC ACTGGGAATC 
GCTCTCGTTC TGCTCATCCA TCCTGGAAAT CCGGATCTGC ACAATGCGGA CGATCGATCT 
ACAGATCGGC GGGCAGTTAA CCTTCTAGAT AGTCTTTTGG ATTTGGGCAG GAACGTATTC 
CCGGACAACC TGTTTCAGGC CTCCATTCAG CAGGCGCACA CCGTCTACTT GCCCAAGCCG 
AGTATCCTGC ACGTCTTCAA CGAAACGATG AATGACACCT TGGCATCCGG ATCGGAGGCC 
CAACGCTTGT CTGAAGACCT TACGGAGGAT GTGGTGCTGG TGAGGGACAT CCAGTACCGC 
AGTGGAACCA ACACCCTGGG CATCGTCTTC TTCTGCCTGG TGTTTGGTAC TTTTTTGGGA 
ACCATCGGCC AGAAGGGTCA GGTCGTGGTT GACTTCTTTG CCGCCATTTT TGAGGTGATC 
ATGAAGATGG TCACCTGCGT GATGTGGCTG ACACCCGTGG GCATTAGTTC TGTGATCGCT 
GGGAAGATAC TGAGCGTGGG CGATTTGGGC CTGGTGATGT CGCAACTCAT GTGGTTCATC 
GTCACGGTGG CCATCGGCGT CTTTATCTAC CAGTTTGTGG TGATGCAGGC CATATATTTC 
GTCGTCGTGC GTCGAAATCC GTTCAAGTTC TACGCCGGAC TCATCCAGGC CATGCTTACC 
GCCTTCGCCA CGGCCTCAAC CGCCGCTGCC CTGCCCATTA CATTCCGCTG CATGAACGAG 
AAACTCAAGG TGGATCCGCG GATCACGCGA TTTGTCCTTC CAATCGGATG CAACATCAAC 
ATGGACGGAA CGGCCCTATA TATCGCAGTG GCATCGATAT TCATTGCCCA GATGAGCGGC 
ATGGTTCTGG GATTTGGAGA GCTCCTTACG GTCCTTCTGA CCTCCACGGC TGCCTCCATG 
AGCTCGGCCA GCGTTCCGAG TGCTGCCCTG GTACTCCTTC TCGTGGTGCT GACCGCCATT 
GATGCGCCTG TTCAGGATGT CACGCTGCTC TTCGCCGTGG ACTGGTTTGT GGATCGGATT 
CGCACCACCA ATAATATGCT GGGTGACTGC TACACTGCTG CCGTAGTCGA GGAGTTGTCG 
CGAAAGGAGT TGATGGCTTT GGATGCCGCT TCCGTTAACT ATCAGGACAT GCCCGCTGGC 
ACCCCCAATG GACATGGACA TGGACATGGA CACCACGATG GAGGACTTCT CGAGGGGCAG 
ACCGAGTTGG AGACGAGCAG CAAGTGCGTT ATGACAATGA CGGACTCGGT GGTGGTGGAC 
ATTAGTGCGG TGATGAATAA CGTGAACCTG CAGCAGGAGC ATTGTAACCG CAGGGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Eaat2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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