ds cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ds
Entrez Gene ID	33245
Full Name	dachsous
Synonyms	CG17941,CT39575,Dmel\CG17941,Ds,EP-822,cad2,dachs,l(2)01855,l(2)05142
General protein information	Preferred Names dachsous Names CG17941 gene product from transcript CG17941-RA CG17941-PA CG17941-PB cadherin-2 daschous ds-PA ds-PB lethal (2) 01855
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 21E2-21E2\|2-0.3 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_078722.3	NP_523446.2	dachsous, isoform A
NM_001298622.1	NP_001285551.1	dachsous, isoform B

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DCHS1	XP_003313012.1
Bos taurus (cattle)	DCHS1	XP_002693195.1
Rattus norvegicus (Norway rat)	Dchs1	NP_001101014.2
Homo sapiens (human)	DCHS1	NP_003728.1
Danio rerio (zebrafish)	dchs1b	XP_001921284.3
	DCHS1	XP_001100797.1
Mus musculus (house mouse)	Dchs1	NP_001156415.1
Gallus gallus (chicken)	DCHS1	XP_417264.4
Caenorhabditis elegans (roundworm)	cdh-1	NP_497641.2
Canis lupus familiaris (dog)	DCHS1	XP_853733.2
Drosophila melanogaster (fruit fly)	ds	NP_523446.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007905	XP_317578.4
Danio rerio (zebrafish)	LOC100334632	XP_002664697.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ds imbalance within the migrating tissue provides the directional cue.
Title: Difference in Dachsous Levels between Migrating Cells Coordinates the Direction of Collective Cell Migration.

Dlish (CG10933) tethers Dachs to the subapical cell cortex, an effect partly mediated by the palmitoyltransferase Approximated under the control of Fat. Conversely, Dlish promotes the Fat-mediated degradation of Dachs
Title: The novel SH3 domain protein Dlish/CG10933 mediates fat signaling in Drosophila by binding and regulating Dachs.

Cell Death Changes the Orientation of Cell Division in the Developing Drosophila Wing Imaginal Disc Without Using Fat or Dachsous as Orienting Signals
Title: Local Cell Death Changes the Orientation of Cell Division in the Developing Drosophila Wing Imaginal Disc Without Using Fat or Dachsous as Orienting Signals.

novel isoforms of dachsous genes expressed during development in Drosophila
Title: Transcriptional analysis of the dachsous gene uncovers novel isoforms expressed during development in Drosophila.

propose a mechanism by which the left-right asymmetry (LR) determinant Myosin ID (MyoID) transfers LR information to neighboring cells through the planar cell polarity atypical cadherin Dachsous
Title: The Atypical Cadherin Dachsous Controls Left-Right Asymmetry in Drosophila.

The following ds gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ds cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa13379	NM_078722.3 Latest version!	Drosophila melanogaster dachsous (ds), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFa13379	NM_001298622.1 Latest version!	Drosophila melanogaster dachsous (ds), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ds ( NM_078722.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523446.2 Drosophila melanogaster dachsous (ds), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa13379
Clone ID Related Accession (Same CDS sequence)	NM_078722.3 , NM_001298622.1
Accession Version	NM_078722.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	dachsous, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_078722.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAGCCA CGTTGGATTC GGTAGTCGCC AGCGAAACGA TGACGAGGGT AACATTCACT 
GGGAACCACG AGTCGAAGGA CTTTGCAATA TTCCATCGAT GCCGGAGACG ATCCAACATC 
AAATCGCTAT ATTCGCCACC TGCCCTGCAT CCCTCGCACA TGCTGCGAAG TAGCTTACTA 
ATTCTACTGG CAATCGTTCT CCTGGGATCT TCGCAGGCTG CCTCACACGA TCAGGAGAGG 
GAGCGAAAGC TGGAAGTCTT CGAAGGCGTT GCCGTAGACT ATCAAATCGG TTATATAGGA 
GACTTCGGCG GAATCGATAG TGGTCCGCCA TACATTATTG TGGCGGAGGC AGGAGTAGAA 
ACAGATCTCG CCATAGATCG GGCCACTGGA GAGATCCGCA CAAAGGTCAA ACTGGACAGA 
GAAACTCGAG CTTCTTATAG TCTAGTGGCT ATTCCCCTGA GTGGTCGTAA TATTCGAGTG 
CTGGTCACAG TTAAAGATGA GAATGACAAT GCCCCCACGT TTCCACAAAC CTCGATGCAC 
ATAGAGTTTC CAGAAAATAC CCCAAGGGAG GTAAAGAGAA CTCTTCTTCC AGCAAGGGAT 
CTGGATCTGG AACCCTACAA CACTCAGAGA TATAATATCG TGTCGGGAAA CGTAAATGAC 
GCCTTTAGAT TGTCGTCCCA CCGAGAAAGG GACGGTGTCC TGTACTTGGA TCTGCAGATC 
AGTGGTTTTT TAGATAGAGA GACCACGCCA GGCTACAGCC TTTTAATTGA AGCTTTAGAT 
GGAGGAACAC CCCCGTTGCG TGGATTTATG ACTGTTAATA TTACCATTCA GGATGTAAAT 
GACAATCAAC CGATCTTCAA TCAGAGCAGG TACTTCGCAA CGGTGCCAGA GAATGCCACT 
GTGGGCACCA GTGTCCTGCA GGTGTATGCA TCAGACACGG ATGCCGATGA GAATGGTTTG 
GTGGAATACG CCATCAACAG AAGACAGAGC GACAAGGAGC AAATGTTTCG CATAGATCCA 
AGAACAGGAG CGATTTACAT CAATAAAGCA CTGGATTTCG AGACTAAGGA GCTGCATGAA 
CTTGTGGTGG TGGCCAAGGA TCATGGTGAA CAGCCGCTGG AGACAACCGC CTTTGTTTCC 
ATCAGAGTCA CAGATGTAAA TGACAATCAA CCGACTATAA ATGTGATCTT CCTGAGTGAC 
GATGCCTCTC CAAAAATATC GGAGTCGGCC CAACCGGGCG AATTCGTGGC CAGAATTTCG 
GTGCATGACC CCGATTCGAA AACGGAGTAT GCCAATGTGA ATGTGACACT CAATGGAGGA 
GATGGTCACT TCGCGTTGAC CACCAGGGAT AACAGTATTT ATTTGGTTAT AGTTCACCTG 
CCACTGGACA GGGAGATAGT TTCAAATTAT ACCCTGAGTG TTGTAGCCAC CGATAAGGGA 
ACTCCGCCCC TGCACGCCTC CAAATCGATC TTCCTAAGGA TTACCGATGT CAATGACAAT 
CCACCGGAGT TCGAGCAGGA CCTCTACCAC GCCAACGTCA TGGAGGTGGC CGATCCGGGT 
ACATCGGTAC TTCAAGTTCT AGCCCACGAT CGAGATGAGG GCCTAAACTC GGCTTTGACA 
TACTCGCTGG CCGAAACGCC TGAAACCCAT GCCCAGTGGT TTCAGATTGA CCCACAAACG 
GGCCTGATCA CCACCCGATC GCATATCGAT TGCGAAACGG AGCCGGTGCC GCAGCTAACG 
GTTGTCGCTA GGGACGGAGG TGTTCCGCCC CTTTCCTCCA CAGCCACGGT CCTTGTTACC 
ATTCACGATG TAAACGACAA CGAACCGATC TTCGACCAGA GCTTCTACAA CGTATCCGTC 
GCTGAAAACG AACCCGTGGG CAGGTGCATA CTCAAGGTCT CTGCAAGTGA TCCAGATTGC 
GGAGTAAATG CCATGGTGAA CTACACCATC GGCGAGGGCT TCAAGCACCT CACCGAATTC 
GAGGTGCGCT CCGCTTCTGG CGAGATTTGC ATAGCTGGCG AACTTGACTT CGAGCGGAGG 
AGTAGCTATG AATTTCCCGT ACTGGCAACC GATCGCGGGG GTCTGAGCAC TACTGCCATG 
ATTAAAATGC AATTGACGGA CGTGAACGAC AATCGTCCGG TTTTCTATCC CAGGGAATAT 
AAAGTATCGC TCAGGGAATC CCCGAAAGCC TCCTCACAAG CTTCGAGTAC TCCGATTGTC 
GCTGTGGTGG CCACAGATCC GGATTACGGA AACTTTGGCC AGGTCTCATA TAGGATTGTG 
GCCGGTAACG AGGCGGGCAT CTTCCGGATT GATCGATCCA CTGGTGAAAT ATTCGTGGTC 
CGTCCGGATA TGCTATCCGT TCGTACCCAA CCCATGCACA TGCTAAATAT ATCGGCGACC 
GATGGCGGTA ATCTAAGGAG CAATGCCGAC GCCGTGGTCT TCTTGAGCAT CATCGACGCC 
ATGCAGCGAC CGCCGATCTT CGAAAAGGCC AGATACAATT ACTATGTGAA GGAGGACATT 
CCGAGGGGCA CGGTTGTGGG TTCTGTGATA GCTGCGAGTG GAGATGTCGC CCATCGAAGT 
CCGGTTAGGT ATTCGATATA CTCGGGCGAT CCCGATGGCT ATTTTAGCAT AGAAACCAAC 
TCTGGAAACA TACGTATAGC TAAGCCCCTG GATCATGAAG CGAAGTCCCA GGTTCTACTC 
AACATCCAAG CCACCTTGGG AGAACCTCCG GTTTATGGAC ACACACAGGT TAATATCGAA 
GTGGAGGATG TCAACGATAA TGCACCTGAG TTCGAGGCCA GTATGGTTAG GATTTCCGTA 
CCGGAAAGTG CAGAGCTTGG AGCTCCACTA TATGCTGCAC ATGCCCACGA TAAAGATTCG 
GGAAGCAGTG GTCAAGTTAC ATATAGCCTG GTCAAGGAAT CGGGGAAAGG ACTCTTTGCC 
ATCGACGCGA GATCTGGCCA CTTAATTTTG TCCCAACACC TAGATTACGA GAGCTCCCAA 
CGACACACCC TAATTGTAAC CGCCACGGAT GGTGGAGTTC CCAGCCTTTC GACGAACTTA 
ACCATCTTGG TGGATGTACA GGATGTCAAC GATAACCCTC CAGTTTTTGA AAAGGACGAG 
TATTCTGTGA ATGTGTCCGA ATCGCGATCG ATCAATGCTC AGATTATTCA AGTTAATGCC 
AGCGATCTGG ACACGGGCAA CAATGCCAGG ATCACCTATA GGATTGTCGA TGCCGGAGTG 
GACAACGTCA CGAACTCCAT TAGTAGCTCA GATGTGTCGC AACATTTTGG CATATTTCCA 
AACTCCGGCT GGATATATTT GCGTGCTCCT CTGGATCGAG AGACCAGGGA TCGCTATCAA 
CTGACGGTTT TGGCCACGGA CAATGGCACT CCCGCTGCTC ACGCAAAAAC ACGTGTCATA 
GTTCGTGTTC TTGATGCCAA CGATAATGAT CCCAAGTTCC AGAAATCTAA ATACGAATTT 
AGGATAGAGG AGAACCTCAG GAGAGGCTCC GTTGTGGGAG TGGTGACCGC CAGTGATCTG 
GATCTGGGTG AGAATGCCGC CATAAGATAC AGCCTGCTGC CGATCAACTC CAGCTTCCAG 
GTGCATCCCG TCACAGGAGA AATTTCCACC CGAGAGCCTC TGGATCGAGA GCTCCGGGAG 
CTGTACGACC TTGTAGTGGA GGCCCGTGAT CAAGGAACTC CAGTGAGAAG TGCTCGCGTC 
CCTGTACGCA TCCACGTAAG CGATGTCAAC GATAACGCAC CCGAGATCGC AGATCCGCAG 
GAGGATGTGG TGAGCGTAAG AGAGGAGCAA CCACCCGGAA CTGAAGTGGT GCGAGTTCGA 
GCCGTCGATC GGGACCACGG GCAGAATGCC TCCATTACAT ATTCAATTGT CAAGGGACGG 
GACTCTGATG GGCACGGACT GTTCAGCATC GATCCCACCA GCGGAGTGAT CCGAACCCGA 
GTGGTTTTGG ATCACGAGGA AAGGAGCATC TATCGCCTGG GTGTGGCCGC CTCCGACGGC 
GGCAATCCGC CCAGGGAGAC GGTACGGATG TTGCGGGTTG AAGTTTTGGA TCTGAACGAC 
AACCGCCCAA CATTTACCAG TTCCAGCTTG GTCTTCAGGG TACGCGAGGA TGCCGCCCTT 
GGCCATGTGG TCGGTTCCAT CAGTCCCATC GAACGTCCGG CGGATGTCGT CAGAAATAGC 
GTGGAGGAGT CCTTCGAAGA TCTGCGCGTC ACCTACACTC TAAATCCCTT GACCAAGGAC 
CTGATCGAGG CTGCCTTCGA CATCGATCGG CATTCGGGAA ACCTGGTCGT GGCTCGTCTC 
TTGGACAGAG AGGTTCAATC CGAATTTAGG TTGGAAATTA GGGCTTTAGA TACCACTGCT 
AGTAATAACC CGCAGAGCAG CGCCATCACG GTTAAGATCG AGGTGGCCGA TGTCAATGAT 
AATGCCCCTG AATGGCCACA GGATCCCATC GACCTTCAGG TGAGCGAAGC CACTCCAGTT 
GGCACAATAA TACACAACTT TACGGCCACG GATGCGGACA CGGGAACCAA TGGGGACCTA 
CAGTATCGAT TGATCCGATA CTTCCCCCAA CTCAATGAAA GCCAAGAGCA GGCAATGAGT 
CTTTTTAGAA TGGATTCCCT GACGGGAGCA CTCAGTCTGC AGGCTCCTTT GGACTTCGAG 
GCTGTGCAGG AGTACCTTCT TATTGTCCAG GCACTGGATC AATCCTCGAA TGTAACAGAA 
AGACTGCAGA CTTCGGTGAC CGTGAGATTG CGAATTCTAG ATGCCAACGA CCATGCCCCG 
CACTTTGTTT CACCCAATTC AAGTGGTGGC AAAACGGCAT CTCTTTTCAT AAGTGATGCA 
ACTAGAATCG GAGAGGTCGT GGCGCACATT GTGGCCGTTG ATGAAGATTC CGGGGACAAT 
GGACAACTTA CCTACGAAAT CACGGGGGGC AATGGGGAAG GCCGCTTTAG AATCAATTCC 
CAAACAGGAA TAATTGAGCT GGTTAAGTCC CTGCCACCGG CCACCGAGGA CGTGGAAAAA 
GGTGGACGGT TTAATCTAAT CATTGGTGCA AAAGATCATG GTCAACCGGA GCCCAAGAAA 
AGCTCTCTAA ATCTGCACCT TATCGTTCAA GGCAGCCACA ATAATCCACC CCGATTTCTG 
CAGGCTGTTT ATCGAGCCAC AATTTTGGAG AACGTTCCTA GCGGAAGTTT CGTCCTGCAA 
GTTACGGCAA AATCACTACA TGGTGCAGAA AATGCTAATT TAAGCTATGA AATTCCTGCT 
GGCGTGGCAA ATGATCTGTT CCACGTGGAC TGGCAGAGGG GCATCATTAC CACACGGGGA 
CAGTTTGACC GGGAGTCCCA GGCAAGTTAT GTCCTGCCCG TCTACGTACG GGATGCCAAC 
CGGCAGTCGA CTTTGTCCTC TTCAGCGGTG AGAAAGCAAC GCTCGTCGGA CAGCATCGGA 
GATACATCGA ATGGCCAGCA CTTTGATGTG GCCACAATTT ATATAACGGT AGGAGATGTC 
AACGACAACT CCCCGGAATT CCGACCAGGG TCATGTTATG GTCTATCAGT CCCAGAGAAC 
AGTGAACCCG GTGTAATTCA CACTGTGGTG GCCAGCGATC TGGATGAAGG TCCGAATGCG 
GATCTTATTT ACAGTATTAC GGGCGGCAAT TTGGGCAATA AGTTCAGCAT TGATTCTAGT 
TCAGGAGAGC TAAGTGCCCG ACCCTTGGAT AGAGAACAGC ACTCTCGGTA CACCCTGCAG 
ATTCAGGCCT CCGATCGGGG GCAACCGAAG AGCCGTCAAG GACACTGCAA CATCACCATA 
TTCGTCGAGG ATCAGAACGA CAATGCCCCG AGATTCAAGC TGAGCAAATA TACTGGCAGT 
GTGCAGGAGG ACGCACCACT GGGCACCAGT GTGGTGCAGA TCAGTGCGGT GGATGCGGAT 
TTGGGCGTCA ACGCCAGACT CGTTTACTCG CTGGCCAATG AAACGCAATG GCAGTTTGCC 
ATCGACGGCC AGAGCGGATT AATCACCACT GTGGGAAAAC TGGATCGCGA GCTGCAGGCG 
AGCTACAACT TCATGGTATT GGCCACGGAC GGAGGACGTT ATGAAGTGAG ATCAGCTACG 
GTTCCGGTGC AGATCAACGT GCTGGATATT AATGACAACA GACCCATTTT CGAGCGATAT 
CCCTATATAG GCCAGGTCCC AGCTCTCATC CAACCTGGCC AGACTTTGCT GAAGGTTCAA 
GCCCTAGATG CAGATTTGGG TGCGAATGCC GAGATTGTGT ATTCCCTCAA TGCTGAAAAT 
TCAGCAGTAA GTGCCAAGTT TAGAATAAAT CCAAGCACTG GTGCCTTGAG TGCCTCTCAG 
AGTTTGGCCA GTGAATCTGG GAAGCTTTTA CACCTGGAGG TCGTTGCCAG GGATAAGGGA 
AATCCGCCTC AATCCAGCCT GGGCCTCATA GAACTTCTAA TAGGGGAAGC ACCGCAAGGA 
ACTCCAGTTC TTAGGTTCCA GAATGAGACC TATCGAGTAA TGCTCAAGGA GAATTCTCCA 
TCGGGTACAA GACTTCTTCA AGTGGTTGCA CTGCGCAGTG ATGGCCGCCG GCAAAAGGTG 
CAATTCTCAT TTGGGGCTGG TAATGAAGAT GGGATTTTGT CCTTAGACTC ACTCTCAGGT 
GAAATAAGGG TGAATAAGCC CCATTTACTT GATTACGACC GCTTCTCCAC ACCCTCAATG 
TCAGCCTTAA GTCGTGGCAG AGCTCTGCAT TATGAGGAGG AAATCGATGA ATCATCAGAG 
GAGGATCCAA ATAATAGCAC CCGATCGCAA AGGGCCTTGA CGTCCTCATC TTTCGCCCTG 
ACCAACAGCC AACCCAATGA AATACGTGTG GTCTTAGTTG CGCGCACCGC AGATGCTCCA 
TTCCTGGCCA GCTATGCGGA ACTAGTGATT GAGCTTGAAG ACGAGAACGA CAATAGCCCA 
AAGTTTTCGC AAAAGCAATT TGTGGCAACG GTTTCAGAAG GAAACAACAA GGGAACCTTT 
GTGGCACAAG TTCATGCATT CGATTCGGAT GCGGGCTCCA ACGCCCGTCT GCGATATCAC 
ATCGTTGATG GCAACCACGA CAATGCATTC GTTATTGAAC CCGCCTTCAG TGGCATAGTG 
AGGACCAACA TAGTGCTGGA TCGGGAGATT CGCGATATAT ATAAGCTCAA GATAATTGCA 
ACCGATGAGG GAGTGCCTCA AATGACTGGC ACGGCCACAA TAAGAGTGCA GATCGTCGAT 
GTGAATGACA ACCAGCCCAC TTTTCCGCCA AACAATCTCG TCACAGTCAG TGAAGCTACT 
GAACTTGGAG CCGTAATAAC CTCTATTTCA GCCAACGATG TGGACACTTA CCCCGCCCTG 
ACCTACCGCC TTGGCGCAGA GTCTACCGTG GATATAGAAA ACATGTCCAT CTTTGCCTTG 
GATCGATATA GCGGGAAGCT CGTTCTAAAA CGTCGGCTGG ACTATGAGCT CCAGCAGGAG 
TATGAACTCG ACGTGATTGC TTCGGATGCA GCCCATGAAG CTCGCACTGT TCTAACTGTG 
CGAGTGAACG ATGAAAACGA CAATGCACCA GTATTCCTGG CCCAACAGCC TCCGGCTTAC 
TTTGCCATTT TGCCAGCTAT ATCCGAGATC AGTGAGAGTC TTTCCGTGGA CTTCGATCTC 
CTAACTGTCA ACGCCACTGA CGCAGACTCG GAGGGTAATA ACTCAAAGGT TATTTATATC 
ATTGAACCTG CCCAGGAGGG CTTCAGTGTG CACCCATCAA ATGGTGTTGT GTCTGTTAAT 
ATGTCTAGAT TACAGCCGGC CGTATCCTCT AGCGGGGATT ATTTTGTAAG AATTATAGCC 
AAGGACGCAG GAAAACCAGC TTTGAAAAGC TCAACACTTT TGAGAGTACA GGCAAATGAC 
AACGGTTCCG GACGTTCGCA ATTCCTACAG AACCAATATA GGGCTCAGAT AAGCGAGGCA 
GCTCCTTTGG GATCGGTTGT TCTCCAGCTT GGACAAGATG CTCTGGATCA ATCCTTAGCT 
ATCATTGCTG GAAATGAAGA ATCCGCTTTT GAGTTGCTCC AATCCAAAGC AATTGTCTTG 
GTCAAGCCCT TGGATAGAGA ACGCAATGAT CTCTACAAAC TGCGATTGGT TTTAAGCCAT 
CCACATGGAC CTCCACTTAT TTCAAGCTTA AATTCCTCGT CTGGAATCTC AGTAATCATA 
ACAATCCTGG ATGCCAACGA TAACTTCCCA ATTTTCGATC GAAGCGCTAA ATATGAGGCT 
GAGATCAGCG AACTTGCTCC TCTGCGGTAC TCCATAGCGC AGTTGCAGGC CATTGATGCC 
GATCAGGAAA ACACCCCAAA CTCAGAAGTG GTATACGACA TAACCTCTGG CAATGATGAG 
CATATGTTTA CGATAGACTT GGTCACCGGC GTTCTCTTTG TGAACAATCG CTTGGACTAC 
GATTCGGGGG CCAAGAGTTA CGAGCTAATA ATCCGAGCCT GCGATAGTCA TCACCAAAGA 
CCACTTTGCA GCTTGCAACC CTTTCGATTG GAACTCCATG ACGAGAACGA CAACGAACCC 
AAGTTCCCGC TAACTGAATA TGTTCACTTC CTGGCTGAAA ACGAGCCGGT TGGCAGCTCC 
GTATTCCGTG CTCATGCCAG TGATTTGGAT AAGGGACCCT TCGGGCAATT AAACTACAGC 
ATAGGTCCTG CTCCCAGCGA CGAGAGCTCT TGGAAAATGT TCCGAGTTGA TTCGGAAAGT 
GGTCTCGTTA CATCCGCCTT CGTATTCGAC TATGAGCAAA GACAGCGCTA TGACATGGAG 
CTGCTCGCCT CCGATATGGG CGGTAAGAAG GCCAGTGTGG CTGTGCGCGT AGAGATTGAG 
TCGAGGGATG AGTTCACCCC GCAGTTCACA GAGCGCACCT ATAGGTTTGT GCTGCCAGCC 
GCCGTGGCCC TACCCCAAGG CTACGTGGTG GGTCAAGTGA CTGCCACTGA TAGTGACAGT 
GGCCCGGACG GACGAGTGGT CTATCAACTA AGTGCCCCAC ACTCGCATTT CAAGGTAAAC 
CGTAGCAGCG GGGCTGTTCT AATCAAGAGG AAACTCAAAC TGGATGGCGA CGGAGACGGT 
AATCTGTACA TGGATGGTCG AGATATCAGT CTAGTTATCT CAGCTTCCTC GGGGCGTCAC 
AACTCCTTAT CCAGCATGGC TGTTGTCGAG ATTGCCTTGG ATCCTTTGGC ACATCCAGGC 
ACAAACCTGG CCAGTGCAGG TGGTTCGTCA AGTGGTTCCA TTGGCGATTG GGCAATTGGT 
CTACTGGTAG CTTTTCTTCT AGTCCTCTGC GCTGCCGCCG GAATCTTTCT ATTCATCCAT 
ATGAGAAGCA GAAAACCGCG TAATGCGGTC AAGCCTCATC TTGCCACGGA CAATGCTGGT 
GTGGGCAATA CCAACAGCTA TGTGGACCCC AGTGCTTTCG ACACGATCCC TATAAGAGGA 
AGCATATCGG GCGGTGCTGC AGGAGCGGCA TCTGGTCAAT TCGCTCCTCC GAAGTACGAC 
GAGATTCCTC CGTTTGGAGC TCATGCTGGC AGCTCCGGAG CAGCTACAAC CTCCGAGTTG 
TCGGGCTCCG AACAGTCCGG TTCCAGTGGC CGAGGCTCTG CTGAGGACGA TGGGGAGGAT 
GAGGAGATTC GTATGATCAA CGAGGGACCA CTACATCACC GCAATGGTGG AGCTGGGGCA 
GGCAGCGATG ATGGCCGAAT TTCGGATATA TCTGTGCAAA ACACCCAGGA ATACTTGGCT 
CGCCTGGGCA TCGTAGATCA CGATCCTAGT GGCGCCGGAG GAGGAGCTTC CAGTATGGCT 
GGTTCAAGTC ACCCAATGCA CTTGTATCAC GATGATGATG CCACAGCACG GTCGGATATT 
ACGAACCTCA TCTATGCCAA ACTTAATGAT GTGACAGGAG CTGGTTCAGA GATAGGAAGC 
AGTGCAGACG ACGCGGGAAC CACTGCGGGC TCCATCGGAA CCATCGGAAC GGCTATAACC 
CATGGCCATG GGGTGATGAG CAGTTACGGA GAAGTTCCTG TTCCAGTACC GGTTGTGGTT 
GGCGGTAGTA ATGTGGGTGG TTCCCTGAGC TCCATAGTTC ACAGTGAGGA GGAATTGACT 
GGCAGCTACA ACTGGGATTA CCTATTGGAT TGGGGACCGC AATATCAGCC ACTTGCGCAT 
GTGTTTTCCG AAATAGCGAG ACTGAAAGAT GACACCTTAT CGGAGCACAG TGGAAGTGGG 
GCTTCCTCGT CCGCCAAGAG CAAGCACTCT TCCTCACATT CCTCAGCAGG AGCAGGAAGC 
GTGGTGCTCA AACCACCACC CTCAGCACCA CCGACTCACA TACCACCCCC CCTGCTAACC 
AATGTGGCAC CAAGGGCCAT TAACCTGCCT ATGCGCTTGC CACCCCATTT GAGCCTGGCC 
CCCGCCCATT TGCCCAGATC GCCCATTGGT CATGAGGCGA GTGGCAGTTT CAGCACATCA 
TCAGCAATGT CACCTTCGTT CTCGCCCTCT CTATCGCCGT TGGCAACAAG ATCACCATCC 
ATTTCTCCTC TGGGAGCGGG ACCACCCACG CACCTGCCTC ATGTCTCGTT GCCACGCCAC 
GGTCATGCCC CTCAGCCAAG TCAGAGGGGA AATGTGGGGA CACGGATGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523446.2
CDS	811..11481
Translation	MKATLDSVVASETMTRVTFTGNHESKDFAIFHRCRRRSNIKSLYSPPALHPSHMLRSSLLILLAIVLLGSSQAASHDQERERKLEVFEGVAVDYQIGYIGDFGGIDSGPPYIIVAEAGVETDLAIDRATGEIRTKVKLDRETRASYSLVAIPLSGRNIRVLVTVKDENDNAPTFPQTSMHIEFPENTPREVKRTLLPARDLDLEPYNTQRYNIVSGNVNDAFRLSSHRERDGVLYLDLQISGFLDRETTPGYSLLIEALDGGTPPLRGFMTVNITIQDVNDNQPIFNQSRYFATVPENATVGTSVLQVYASDTDADENGLVEYAINRRQSDKEQMFRIDPRTGAIYINKALDFETKELHELVVVAKDHGEQPLETTAFVSIRVTDVNDNQPTINVIFLSDDASPKISESAQPGEFVARISVHDPDSKTEYANVNVTLNGGDGHFALTTRDNSIYLVIVHLPLDREIVSNYTLSVVATDKGTPPLHASKSIFLRITDVNDNPPEFEQDLYHANVMEVADPGTSVLQVLAHDRDEGLNSALTYSLAETPETHAQWFQIDPQTGLITTRSHIDCETEPVPQLTVVARDGGVPPLSSTATVLVTIHDVNDNEPIFDQSFYNVSVAENEPVGRCILKVSASDPDCGVNAMVNYTIGEGFKHLTEFEVRSASGEICIAGELDFERRSSYEFPVLATDRGGLSTTAMIKMQLTDVNDNRPVFYPREYKVSLRESPKASSQASSTPIVAVVATDPDYGNFGQVSYRIVAGNEAGIFRIDRSTGEIFVVRPDMLSVRTQPMHMLNISATDGGNLRSNADAVVFLSIIDAMQRPPIFEKARYNYYVKEDIPRGTVVGSVIAASGDVAHRSPVRYSIYSGDPDGYFSIETNSGNIRIAKPLDHEAKSQVLLNIQATLGEPPVYGHTQVNIEVEDVNDNAPEFEASMVRISVPESAELGAPLYAAHAHDKDSGSSGQVTYSLVKESGKGLFAIDARSGHLILSQHLDYESSQRHTLIVTATDGGVPSLSTNLTILVDVQDVNDNPPVFEKDEYSVNVSESRSINAQIIQVNASDLDTGNNARITYRIVDAGVDNVTNSISSSDVSQHFGIFPNSGWIYLRAPLDRETRDRYQLTVLATDNGTPAAHAKTRVIVRVLDANDNDPKFQKSKYEFRIEENLRRGSVVGVVTASDLDLGENAAIRYSLLPINSSFQVHPVTGEISTREPLDRELRELYDLVVEARDQGTPVRSARVPVRIHVSDVNDNAPEIADPQEDVVSVREEQPPGTEVVRVRAVDRDHGQNASITYSIVKGRDSDGHGLFSIDPTSGVIRTRVVLDHEERSIYRLGVAASDGGNPPRETVRMLRVEVLDLNDNRPTFTSSSLVFRVREDAALGHVVGSISPIERPADVVRNSVEESFEDLRVTYTLNPLTKDLIEAAFDIDRHSGNLVVARLLDREVQSEFRLEIRALDTTASNNPQSSAITVKIEVADVNDNAPEWPQDPIDLQVSEATPVGTIIHNFTATDADTGTNGDLQYRLIRYFPQLNESQEQAMSLFRMDSLTGALSLQAPLDFEAVQEYLLIVQALDQSSNVTERLQTSVTVRLRILDANDHAPHFVSPNSSGGKTASLFISDATRIGEVVAHIVAVDEDSGDNGQLTYEITGGNGEGRFRINSQTGIIELVKSLPPATEDVEKGGRFNLIIGAKDHGQPEPKKSSLNLHLIVQGSHNNPPRFLQAVYRATILENVPSGSFVLQVTAKSLHGAENANLSYEIPAGVANDLFHVDWQRGIITTRGQFDRESQASYVLPVYVRDANRQSTLSSSAVRKQRSSDSIGDTSNGQHFDVATIYITVGDVNDNSPEFRPGSCYGLSVPENSEPGVIHTVVASDLDEGPNADLIYSITGGNLGNKFSIDSSSGELSARPLDREQHSRYTLQIQASDRGQPKSRQGHCNITIFVEDQNDNAPRFKLSKYTGSVQEDAPLGTSVVQISAVDADLGVNARLVYSLANETQWQFAIDGQSGLITTVGKLDRELQASYNFMVLATDGGRYEVRSATVPVQINVLDINDNRPIFERYPYIGQVPALIQPGQTLLKVQALDADLGANAEIVYSLNAENSAVSAKFRINPSTGALSASQSLASESGKLLHLEVVARDKGNPPQSSLGLIELLIGEAPQGTPVLRFQNETYRVMLKENSPSGTRLLQVVALRSDGRRQKVQFSFGAGNEDGILSLDSLSGEIRVNKPHLLDYDRFSTPSMSALSRGRALHYEEEIDESSEEDPNNSTRSQRALTSSSFALTNSQPNEIRVVLVARTADAPFLASYAELVIELEDENDNSPKFSQKQFVATVSEGNNKGTFVAQVHAFDSDAGSNARLRYHIVDGNHDNAFVIEPAFSGIVRTNIVLDREIRDIYKLKIIATDEGVPQMTGTATIRVQIVDVNDNQPTFPPNNLVTVSEATELGAVITSISANDVDTYPALTYRLGAESTVDIENMSIFALDRYSGKLVLKRRLDYELQQEYELDVIASDAAHEARTVLTVRVNDENDNAPVFLAQQPPAYFAILPAISEISESLSVDFDLLTVNATDADSEGNNSKVIYIIEPAQEGFSVHPSNGVVSVNMSRLQPAVSSSGDYFVRIIAKDAGKPALKSSTLLRVQANDNGSGRSQFLQNQYRAQISEAAPLGSVVLQLGQDALDQSLAIIAGNEESAFELLQSKAIVLVKPLDRERNDLYKLRLVLSHPHGPPLISSLNSSSGISVIITILDANDNFPIFDRSAKYEAEISELAPLRYSIAQLQAIDADQENTPNSEVVYDITSGNDEHMFTIDLVTGVLFVNNRLDYDSGAKSYELIIRACDSHHQRPLCSLQPFRLELHDENDNEPKFPLTEYVHFLAENEPVGSSVFRAHASDLDKGPFGQLNYSIGPAPSDESSWKMFRVDSESGLVTSAFVFDYEQRQRYDMELLASDMGGKKASVAVRVEIESRDEFTPQFTERTYRFVLPAAVALPQGYVVGQVTATDSDSGPDGRVVYQLSAPHSHFKVNRSSGAVLIKRKLKLDGDGDGNLYMDGRDISLVISASSGRHNSLSSMAVVEIALDPLAHPGTNLASAGGSSSGSIGDWAIGLLVAFLLVLCAAAGIFLFIHMRSRKPRNAVKPHLATDNAGVGNTNSYVDPSAFDTIPIRGSISGGAAGAASGQFAPPKYDEIPPFGAHAGSSGAATTSELSGSEQSGSSGRGSAEDDGEDEEIRMINEGPLHHRNGGAGAGSDDGRISDISVQNTQEYLARLGIVDHDPSGAGGGASSMAGSSHPMHLYHDDDATARSDITNLIYAKLNDVTGAGSEIGSSADDAGTTAGSIGTIGTAITHGHGVMSSYGEVPVPVPVVVGGSNVGGSLSSIVHSEEELTGSYNWDYLLDWGPQYQPLAHVFSEIARLKDDTLSEHSGSGASSSAKSKHSSSHSSAGAGSVVLKPPPSAPPTHIPPPLLTNVAPRAINLPMRLPPHLSLAPAHLPRSPIGHEASGSFSTSSAMSPSFSPSLSPLATRSPSISPLGAGPPTHLPHVSLPRHGHAPQPSQRGNVGTRM

Target ORF information:

RefSeq Version	NM_078722.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster dachsous (ds), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078722.3

ORF Insert Sequence:

ATGAAAGCCA CGTTGGATTC GGTAGTCGCC AGCGAAACGA TGACGAGGGT AACATTCACT 
GGGAACCACG AGTCGAAGGA CTTTGCAATA TTCCATCGAT GCCGGAGACG ATCCAACATC 
AAATCGCTAT ATTCGCCACC TGCCCTGCAT CCCTCGCACA TGCTGCGAAG TAGCTTACTA 
ATTCTACTGG CAATCGTTCT CCTGGGATCT TCGCAGGCTG CCTCACACGA TCAGGAGAGG 
GAGCGAAAGC TGGAAGTCTT CGAAGGCGTT GCCGTAGACT ATCAAATCGG TTATATAGGA 
GACTTCGGCG GAATCGATAG TGGTCCGCCA TACATTATTG TGGCGGAGGC AGGAGTAGAA 
ACAGATCTCG CCATAGATCG GGCCACTGGA GAGATCCGCA CAAAGGTCAA ACTGGACAGA 
GAAACTCGAG CTTCTTATAG TCTAGTGGCT ATTCCCCTGA GTGGTCGTAA TATTCGAGTG 
CTGGTCACAG TTAAAGATGA GAATGACAAT GCCCCCACGT TTCCACAAAC CTCGATGCAC 
ATAGAGTTTC CAGAAAATAC CCCAAGGGAG GTAAAGAGAA CTCTTCTTCC AGCAAGGGAT 
CTGGATCTGG AACCCTACAA CACTCAGAGA TATAATATCG TGTCGGGAAA CGTAAATGAC 
GCCTTTAGAT TGTCGTCCCA CCGAGAAAGG GACGGTGTCC TGTACTTGGA TCTGCAGATC 
AGTGGTTTTT TAGATAGAGA GACCACGCCA GGCTACAGCC TTTTAATTGA AGCTTTAGAT 
GGAGGAACAC CCCCGTTGCG TGGATTTATG ACTGTTAATA TTACCATTCA GGATGTAAAT 
GACAATCAAC CGATCTTCAA TCAGAGCAGG TACTTCGCAA CGGTGCCAGA GAATGCCACT 
GTGGGCACCA GTGTCCTGCA GGTGTATGCA TCAGACACGG ATGCCGATGA GAATGGTTTG 
GTGGAATACG CCATCAACAG AAGACAGAGC GACAAGGAGC AAATGTTTCG CATAGATCCA 
AGAACAGGAG CGATTTACAT CAATAAAGCA CTGGATTTCG AGACTAAGGA GCTGCATGAA 
CTTGTGGTGG TGGCCAAGGA TCATGGTGAA CAGCCGCTGG AGACAACCGC CTTTGTTTCC 
ATCAGAGTCA CAGATGTAAA TGACAATCAA CCGACTATAA ATGTGATCTT CCTGAGTGAC 
GATGCCTCTC CAAAAATATC GGAGTCGGCC CAACCGGGCG AATTCGTGGC CAGAATTTCG 
GTGCATGACC CCGATTCGAA AACGGAGTAT GCCAATGTGA ATGTGACACT CAATGGAGGA 
GATGGTCACT TCGCGTTGAC CACCAGGGAT AACAGTATTT ATTTGGTTAT AGTTCACCTG 
CCACTGGACA GGGAGATAGT TTCAAATTAT ACCCTGAGTG TTGTAGCCAC CGATAAGGGA 
ACTCCGCCCC TGCACGCCTC CAAATCGATC TTCCTAAGGA TTACCGATGT CAATGACAAT 
CCACCGGAGT TCGAGCAGGA CCTCTACCAC GCCAACGTCA TGGAGGTGGC CGATCCGGGT 
ACATCGGTAC TTCAAGTTCT AGCCCACGAT CGAGATGAGG GCCTAAACTC GGCTTTGACA 
TACTCGCTGG CCGAAACGCC TGAAACCCAT GCCCAGTGGT TTCAGATTGA CCCACAAACG 
GGCCTGATCA CCACCCGATC GCATATCGAT TGCGAAACGG AGCCGGTGCC GCAGCTAACG 
GTTGTCGCTA GGGACGGAGG TGTTCCGCCC CTTTCCTCCA CAGCCACGGT CCTTGTTACC 
ATTCACGATG TAAACGACAA CGAACCGATC TTCGACCAGA GCTTCTACAA CGTATCCGTC 
GCTGAAAACG AACCCGTGGG CAGGTGCATA CTCAAGGTCT CTGCAAGTGA TCCAGATTGC 
GGAGTAAATG CCATGGTGAA CTACACCATC GGCGAGGGCT TCAAGCACCT CACCGAATTC 
GAGGTGCGCT CCGCTTCTGG CGAGATTTGC ATAGCTGGCG AACTTGACTT CGAGCGGAGG 
AGTAGCTATG AATTTCCCGT ACTGGCAACC GATCGCGGGG GTCTGAGCAC TACTGCCATG 
ATTAAAATGC AATTGACGGA CGTGAACGAC AATCGTCCGG TTTTCTATCC CAGGGAATAT 
AAAGTATCGC TCAGGGAATC CCCGAAAGCC TCCTCACAAG CTTCGAGTAC TCCGATTGTC 
GCTGTGGTGG CCACAGATCC GGATTACGGA AACTTTGGCC AGGTCTCATA TAGGATTGTG 
GCCGGTAACG AGGCGGGCAT CTTCCGGATT GATCGATCCA CTGGTGAAAT ATTCGTGGTC 
CGTCCGGATA TGCTATCCGT TCGTACCCAA CCCATGCACA TGCTAAATAT ATCGGCGACC 
GATGGCGGTA ATCTAAGGAG CAATGCCGAC GCCGTGGTCT TCTTGAGCAT CATCGACGCC 
ATGCAGCGAC CGCCGATCTT CGAAAAGGCC AGATACAATT ACTATGTGAA GGAGGACATT 
CCGAGGGGCA CGGTTGTGGG TTCTGTGATA GCTGCGAGTG GAGATGTCGC CCATCGAAGT 
CCGGTTAGGT ATTCGATATA CTCGGGCGAT CCCGATGGCT ATTTTAGCAT AGAAACCAAC 
TCTGGAAACA TACGTATAGC TAAGCCCCTG GATCATGAAG CGAAGTCCCA GGTTCTACTC 
AACATCCAAG CCACCTTGGG AGAACCTCCG GTTTATGGAC ACACACAGGT TAATATCGAA 
GTGGAGGATG TCAACGATAA TGCACCTGAG TTCGAGGCCA GTATGGTTAG GATTTCCGTA 
CCGGAAAGTG CAGAGCTTGG AGCTCCACTA TATGCTGCAC ATGCCCACGA TAAAGATTCG 
GGAAGCAGTG GTCAAGTTAC ATATAGCCTG GTCAAGGAAT CGGGGAAAGG ACTCTTTGCC 
ATCGACGCGA GATCTGGCCA CTTAATTTTG TCCCAACACC TAGATTACGA GAGCTCCCAA 
CGACACACCC TAATTGTAAC CGCCACGGAT GGTGGAGTTC CCAGCCTTTC GACGAACTTA 
ACCATCTTGG TGGATGTACA GGATGTCAAC GATAACCCTC CAGTTTTTGA AAAGGACGAG 
TATTCTGTGA ATGTGTCCGA ATCGCGATCG ATCAATGCTC AGATTATTCA AGTTAATGCC 
AGCGATCTGG ACACGGGCAA CAATGCCAGG ATCACCTATA GGATTGTCGA TGCCGGAGTG 
GACAACGTCA CGAACTCCAT TAGTAGCTCA GATGTGTCGC AACATTTTGG CATATTTCCA 
AACTCCGGCT GGATATATTT GCGTGCTCCT CTGGATCGAG AGACCAGGGA TCGCTATCAA 
CTGACGGTTT TGGCCACGGA CAATGGCACT CCCGCTGCTC ACGCAAAAAC ACGTGTCATA 
GTTCGTGTTC TTGATGCCAA CGATAATGAT CCCAAGTTCC AGAAATCTAA ATACGAATTT 
AGGATAGAGG AGAACCTCAG GAGAGGCTCC GTTGTGGGAG TGGTGACCGC CAGTGATCTG 
GATCTGGGTG AGAATGCCGC CATAAGATAC AGCCTGCTGC CGATCAACTC CAGCTTCCAG 
GTGCATCCCG TCACAGGAGA AATTTCCACC CGAGAGCCTC TGGATCGAGA GCTCCGGGAG 
CTGTACGACC TTGTAGTGGA GGCCCGTGAT CAAGGAACTC CAGTGAGAAG TGCTCGCGTC 
CCTGTACGCA TCCACGTAAG CGATGTCAAC GATAACGCAC CCGAGATCGC AGATCCGCAG 
GAGGATGTGG TGAGCGTAAG AGAGGAGCAA CCACCCGGAA CTGAAGTGGT GCGAGTTCGA 
GCCGTCGATC GGGACCACGG GCAGAATGCC TCCATTACAT ATTCAATTGT CAAGGGACGG 
GACTCTGATG GGCACGGACT GTTCAGCATC GATCCCACCA GCGGAGTGAT CCGAACCCGA 
GTGGTTTTGG ATCACGAGGA AAGGAGCATC TATCGCCTGG GTGTGGCCGC CTCCGACGGC 
GGCAATCCGC CCAGGGAGAC GGTACGGATG TTGCGGGTTG AAGTTTTGGA TCTGAACGAC 
AACCGCCCAA CATTTACCAG TTCCAGCTTG GTCTTCAGGG TACGCGAGGA TGCCGCCCTT 
GGCCATGTGG TCGGTTCCAT CAGTCCCATC GAACGTCCGG CGGATGTCGT CAGAAATAGC 
GTGGAGGAGT CCTTCGAAGA TCTGCGCGTC ACCTACACTC TAAATCCCTT GACCAAGGAC 
CTGATCGAGG CTGCCTTCGA CATCGATCGG CATTCGGGAA ACCTGGTCGT GGCTCGTCTC 
TTGGACAGAG AGGTTCAATC CGAATTTAGG TTGGAAATTA GGGCTTTAGA TACCACTGCT 
AGTAATAACC CGCAGAGCAG CGCCATCACG GTTAAGATCG AGGTGGCCGA TGTCAATGAT 
AATGCCCCTG AATGGCCACA GGATCCCATC GACCTTCAGG TGAGCGAAGC CACTCCAGTT 
GGCACAATAA TACACAACTT TACGGCCACG GATGCGGACA CGGGAACCAA TGGGGACCTA 
CAGTATCGAT TGATCCGATA CTTCCCCCAA CTCAATGAAA GCCAAGAGCA GGCAATGAGT 
CTTTTTAGAA TGGATTCCCT GACGGGAGCA CTCAGTCTGC AGGCTCCTTT GGACTTCGAG 
GCTGTGCAGG AGTACCTTCT TATTGTCCAG GCACTGGATC AATCCTCGAA TGTAACAGAA 
AGACTGCAGA CTTCGGTGAC CGTGAGATTG CGAATTCTAG ATGCCAACGA CCATGCCCCG 
CACTTTGTTT CACCCAATTC AAGTGGTGGC AAAACGGCAT CTCTTTTCAT AAGTGATGCA 
ACTAGAATCG GAGAGGTCGT GGCGCACATT GTGGCCGTTG ATGAAGATTC CGGGGACAAT 
GGACAACTTA CCTACGAAAT CACGGGGGGC AATGGGGAAG GCCGCTTTAG AATCAATTCC 
CAAACAGGAA TAATTGAGCT GGTTAAGTCC CTGCCACCGG CCACCGAGGA CGTGGAAAAA 
GGTGGACGGT TTAATCTAAT CATTGGTGCA AAAGATCATG GTCAACCGGA GCCCAAGAAA 
AGCTCTCTAA ATCTGCACCT TATCGTTCAA GGCAGCCACA ATAATCCACC CCGATTTCTG 
CAGGCTGTTT ATCGAGCCAC AATTTTGGAG AACGTTCCTA GCGGAAGTTT CGTCCTGCAA 
GTTACGGCAA AATCACTACA TGGTGCAGAA AATGCTAATT TAAGCTATGA AATTCCTGCT 
GGCGTGGCAA ATGATCTGTT CCACGTGGAC TGGCAGAGGG GCATCATTAC CACACGGGGA 
CAGTTTGACC GGGAGTCCCA GGCAAGTTAT GTCCTGCCCG TCTACGTACG GGATGCCAAC 
CGGCAGTCGA CTTTGTCCTC TTCAGCGGTG AGAAAGCAAC GCTCGTCGGA CAGCATCGGA 
GATACATCGA ATGGCCAGCA CTTTGATGTG GCCACAATTT ATATAACGGT AGGAGATGTC 
AACGACAACT CCCCGGAATT CCGACCAGGG TCATGTTATG GTCTATCAGT CCCAGAGAAC 
AGTGAACCCG GTGTAATTCA CACTGTGGTG GCCAGCGATC TGGATGAAGG TCCGAATGCG 
GATCTTATTT ACAGTATTAC GGGCGGCAAT TTGGGCAATA AGTTCAGCAT TGATTCTAGT 
TCAGGAGAGC TAAGTGCCCG ACCCTTGGAT AGAGAACAGC ACTCTCGGTA CACCCTGCAG 
ATTCAGGCCT CCGATCGGGG GCAACCGAAG AGCCGTCAAG GACACTGCAA CATCACCATA 
TTCGTCGAGG ATCAGAACGA CAATGCCCCG AGATTCAAGC TGAGCAAATA TACTGGCAGT 
GTGCAGGAGG ACGCACCACT GGGCACCAGT GTGGTGCAGA TCAGTGCGGT GGATGCGGAT 
TTGGGCGTCA ACGCCAGACT CGTTTACTCG CTGGCCAATG AAACGCAATG GCAGTTTGCC 
ATCGACGGCC AGAGCGGATT AATCACCACT GTGGGAAAAC TGGATCGCGA GCTGCAGGCG 
AGCTACAACT TCATGGTATT GGCCACGGAC GGAGGACGTT ATGAAGTGAG ATCAGCTACG 
GTTCCGGTGC AGATCAACGT GCTGGATATT AATGACAACA GACCCATTTT CGAGCGATAT 
CCCTATATAG GCCAGGTCCC AGCTCTCATC CAACCTGGCC AGACTTTGCT GAAGGTTCAA 
GCCCTAGATG CAGATTTGGG TGCGAATGCC GAGATTGTGT ATTCCCTCAA TGCTGAAAAT 
TCAGCAGTAA GTGCCAAGTT TAGAATAAAT CCAAGCACTG GTGCCTTGAG TGCCTCTCAG 
AGTTTGGCCA GTGAATCTGG GAAGCTTTTA CACCTGGAGG TCGTTGCCAG GGATAAGGGA 
AATCCGCCTC AATCCAGCCT GGGCCTCATA GAACTTCTAA TAGGGGAAGC ACCGCAAGGA 
ACTCCAGTTC TTAGGTTCCA GAATGAGACC TATCGAGTAA TGCTCAAGGA GAATTCTCCA 
TCGGGTACAA GACTTCTTCA AGTGGTTGCA CTGCGCAGTG ATGGCCGCCG GCAAAAGGTG 
CAATTCTCAT TTGGGGCTGG TAATGAAGAT GGGATTTTGT CCTTAGACTC ACTCTCAGGT 
GAAATAAGGG TGAATAAGCC CCATTTACTT GATTACGACC GCTTCTCCAC ACCCTCAATG 
TCAGCCTTAA GTCGTGGCAG AGCTCTGCAT TATGAGGAGG AAATCGATGA ATCATCAGAG 
GAGGATCCAA ATAATAGCAC CCGATCGCAA AGGGCCTTGA CGTCCTCATC TTTCGCCCTG 
ACCAACAGCC AACCCAATGA AATACGTGTG GTCTTAGTTG CGCGCACCGC AGATGCTCCA 
TTCCTGGCCA GCTATGCGGA ACTAGTGATT GAGCTTGAAG ACGAGAACGA CAATAGCCCA 
AAGTTTTCGC AAAAGCAATT TGTGGCAACG GTTTCAGAAG GAAACAACAA GGGAACCTTT 
GTGGCACAAG TTCATGCATT CGATTCGGAT GCGGGCTCCA ACGCCCGTCT GCGATATCAC 
ATCGTTGATG GCAACCACGA CAATGCATTC GTTATTGAAC CCGCCTTCAG TGGCATAGTG 
AGGACCAACA TAGTGCTGGA TCGGGAGATT CGCGATATAT ATAAGCTCAA GATAATTGCA 
ACCGATGAGG GAGTGCCTCA AATGACTGGC ACGGCCACAA TAAGAGTGCA GATCGTCGAT 
GTGAATGACA ACCAGCCCAC TTTTCCGCCA AACAATCTCG TCACAGTCAG TGAAGCTACT 
GAACTTGGAG CCGTAATAAC CTCTATTTCA GCCAACGATG TGGACACTTA CCCCGCCCTG 
ACCTACCGCC TTGGCGCAGA GTCTACCGTG GATATAGAAA ACATGTCCAT CTTTGCCTTG 
GATCGATATA GCGGGAAGCT CGTTCTAAAA CGTCGGCTGG ACTATGAGCT CCAGCAGGAG 
TATGAACTCG ACGTGATTGC TTCGGATGCA GCCCATGAAG CTCGCACTGT TCTAACTGTG 
CGAGTGAACG ATGAAAACGA CAATGCACCA GTATTCCTGG CCCAACAGCC TCCGGCTTAC 
TTTGCCATTT TGCCAGCTAT ATCCGAGATC AGTGAGAGTC TTTCCGTGGA CTTCGATCTC 
CTAACTGTCA ACGCCACTGA CGCAGACTCG GAGGGTAATA ACTCAAAGGT TATTTATATC 
ATTGAACCTG CCCAGGAGGG CTTCAGTGTG CACCCATCAA ATGGTGTTGT GTCTGTTAAT 
ATGTCTAGAT TACAGCCGGC CGTATCCTCT AGCGGGGATT ATTTTGTAAG AATTATAGCC 
AAGGACGCAG GAAAACCAGC TTTGAAAAGC TCAACACTTT TGAGAGTACA GGCAAATGAC 
AACGGTTCCG GACGTTCGCA ATTCCTACAG AACCAATATA GGGCTCAGAT AAGCGAGGCA 
GCTCCTTTGG GATCGGTTGT TCTCCAGCTT GGACAAGATG CTCTGGATCA ATCCTTAGCT 
ATCATTGCTG GAAATGAAGA ATCCGCTTTT GAGTTGCTCC AATCCAAAGC AATTGTCTTG 
GTCAAGCCCT TGGATAGAGA ACGCAATGAT CTCTACAAAC TGCGATTGGT TTTAAGCCAT 
CCACATGGAC CTCCACTTAT TTCAAGCTTA AATTCCTCGT CTGGAATCTC AGTAATCATA 
ACAATCCTGG ATGCCAACGA TAACTTCCCA ATTTTCGATC GAAGCGCTAA ATATGAGGCT 
GAGATCAGCG AACTTGCTCC TCTGCGGTAC TCCATAGCGC AGTTGCAGGC CATTGATGCC 
GATCAGGAAA ACACCCCAAA CTCAGAAGTG GTATACGACA TAACCTCTGG CAATGATGAG 
CATATGTTTA CGATAGACTT GGTCACCGGC GTTCTCTTTG TGAACAATCG CTTGGACTAC 
GATTCGGGGG CCAAGAGTTA CGAGCTAATA ATCCGAGCCT GCGATAGTCA TCACCAAAGA 
CCACTTTGCA GCTTGCAACC CTTTCGATTG GAACTCCATG ACGAGAACGA CAACGAACCC 
AAGTTCCCGC TAACTGAATA TGTTCACTTC CTGGCTGAAA ACGAGCCGGT TGGCAGCTCC 
GTATTCCGTG CTCATGCCAG TGATTTGGAT AAGGGACCCT TCGGGCAATT AAACTACAGC 
ATAGGTCCTG CTCCCAGCGA CGAGAGCTCT TGGAAAATGT TCCGAGTTGA TTCGGAAAGT 
GGTCTCGTTA CATCCGCCTT CGTATTCGAC TATGAGCAAA GACAGCGCTA TGACATGGAG 
CTGCTCGCCT CCGATATGGG CGGTAAGAAG GCCAGTGTGG CTGTGCGCGT AGAGATTGAG 
TCGAGGGATG AGTTCACCCC GCAGTTCACA GAGCGCACCT ATAGGTTTGT GCTGCCAGCC 
GCCGTGGCCC TACCCCAAGG CTACGTGGTG GGTCAAGTGA CTGCCACTGA TAGTGACAGT 
GGCCCGGACG GACGAGTGGT CTATCAACTA AGTGCCCCAC ACTCGCATTT CAAGGTAAAC 
CGTAGCAGCG GGGCTGTTCT AATCAAGAGG AAACTCAAAC TGGATGGCGA CGGAGACGGT 
AATCTGTACA TGGATGGTCG AGATATCAGT CTAGTTATCT CAGCTTCCTC GGGGCGTCAC 
AACTCCTTAT CCAGCATGGC TGTTGTCGAG ATTGCCTTGG ATCCTTTGGC ACATCCAGGC 
ACAAACCTGG CCAGTGCAGG TGGTTCGTCA AGTGGTTCCA TTGGCGATTG GGCAATTGGT 
CTACTGGTAG CTTTTCTTCT AGTCCTCTGC GCTGCCGCCG GAATCTTTCT ATTCATCCAT 
ATGAGAAGCA GAAAACCGCG TAATGCGGTC AAGCCTCATC TTGCCACGGA CAATGCTGGT 
GTGGGCAATA CCAACAGCTA TGTGGACCCC AGTGCTTTCG ACACGATCCC TATAAGAGGA 
AGCATATCGG GCGGTGCTGC AGGAGCGGCA TCTGGTCAAT TCGCTCCTCC GAAGTACGAC 
GAGATTCCTC CGTTTGGAGC TCATGCTGGC AGCTCCGGAG CAGCTACAAC CTCCGAGTTG 
TCGGGCTCCG AACAGTCCGG TTCCAGTGGC CGAGGCTCTG CTGAGGACGA TGGGGAGGAT 
GAGGAGATTC GTATGATCAA CGAGGGACCA CTACATCACC GCAATGGTGG AGCTGGGGCA 
GGCAGCGATG ATGGCCGAAT TTCGGATATA TCTGTGCAAA ACACCCAGGA ATACTTGGCT 
CGCCTGGGCA TCGTAGATCA CGATCCTAGT GGCGCCGGAG GAGGAGCTTC CAGTATGGCT 
GGTTCAAGTC ACCCAATGCA CTTGTATCAC GATGATGATG CCACAGCACG GTCGGATATT 
ACGAACCTCA TCTATGCCAA ACTTAATGAT GTGACAGGAG CTGGTTCAGA GATAGGAAGC 
AGTGCAGACG ACGCGGGAAC CACTGCGGGC TCCATCGGAA CCATCGGAAC GGCTATAACC 
CATGGCCATG GGGTGATGAG CAGTTACGGA GAAGTTCCTG TTCCAGTACC GGTTGTGGTT 
GGCGGTAGTA ATGTGGGTGG TTCCCTGAGC TCCATAGTTC ACAGTGAGGA GGAATTGACT 
GGCAGCTACA ACTGGGATTA CCTATTGGAT TGGGGACCGC AATATCAGCC ACTTGCGCAT 
GTGTTTTCCG AAATAGCGAG ACTGAAAGAT GACACCTTAT CGGAGCACAG TGGAAGTGGG 
GCTTCCTCGT CCGCCAAGAG CAAGCACTCT TCCTCACATT CCTCAGCAGG AGCAGGAAGC 
GTGGTGCTCA AACCACCACC CTCAGCACCA CCGACTCACA TACCACCCCC CCTGCTAACC 
AATGTGGCAC CAAGGGCCAT TAACCTGCCT ATGCGCTTGC CACCCCATTT GAGCCTGGCC 
CCCGCCCATT TGCCCAGATC GCCCATTGGT CATGAGGCGA GTGGCAGTTT CAGCACATCA 
TCAGCAATGT CACCTTCGTT CTCGCCCTCT CTATCGCCGT TGGCAACAAG ATCACCATCC 
ATTTCTCCTC TGGGAGCGGG ACCACCCACG CACCTGCCTC ATGTCTCGTT GCCACGCCAC 
GGTCATGCCC CTCAGCCAAG TCAGAGGGGA AATGTGGGGA CACGGATGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ds ( NM_001298622.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285551.1 Drosophila melanogaster dachsous (ds), transcript variant B, mRNA.

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CloneID	OFa13379
Clone ID Related Accession (Same CDS sequence)	NM_078722.3 , NM_001298622.1
Accession Version	NM_001298622.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	dachsous, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAGCCA CGTTGGATTC GGTAGTCGCC AGCGAAACGA TGACGAGGGT AACATTCACT 
GGGAACCACG AGTCGAAGGA CTTTGCAATA TTCCATCGAT GCCGGAGACG ATCCAACATC 
AAATCGCTAT ATTCGCCACC TGCCCTGCAT CCCTCGCACA TGCTGCGAAG TAGCTTACTA 
ATTCTACTGG CAATCGTTCT CCTGGGATCT TCGCAGGCTG CCTCACACGA TCAGGAGAGG 
GAGCGAAAGC TGGAAGTCTT CGAAGGCGTT GCCGTAGACT ATCAAATCGG TTATATAGGA 
GACTTCGGCG GAATCGATAG TGGTCCGCCA TACATTATTG TGGCGGAGGC AGGAGTAGAA 
ACAGATCTCG CCATAGATCG GGCCACTGGA GAGATCCGCA CAAAGGTCAA ACTGGACAGA 
GAAACTCGAG CTTCTTATAG TCTAGTGGCT ATTCCCCTGA GTGGTCGTAA TATTCGAGTG 
CTGGTCACAG TTAAAGATGA GAATGACAAT GCCCCCACGT TTCCACAAAC CTCGATGCAC 
ATAGAGTTTC CAGAAAATAC CCCAAGGGAG GTAAAGAGAA CTCTTCTTCC AGCAAGGGAT 
CTGGATCTGG AACCCTACAA CACTCAGAGA TATAATATCG TGTCGGGAAA CGTAAATGAC 
GCCTTTAGAT TGTCGTCCCA CCGAGAAAGG GACGGTGTCC TGTACTTGGA TCTGCAGATC 
AGTGGTTTTT TAGATAGAGA GACCACGCCA GGCTACAGCC TTTTAATTGA AGCTTTAGAT 
GGAGGAACAC CCCCGTTGCG TGGATTTATG ACTGTTAATA TTACCATTCA GGATGTAAAT 
GACAATCAAC CGATCTTCAA TCAGAGCAGG TACTTCGCAA CGGTGCCAGA GAATGCCACT 
GTGGGCACCA GTGTCCTGCA GGTGTATGCA TCAGACACGG ATGCCGATGA GAATGGTTTG 
GTGGAATACG CCATCAACAG AAGACAGAGC GACAAGGAGC AAATGTTTCG CATAGATCCA 
AGAACAGGAG CGATTTACAT CAATAAAGCA CTGGATTTCG AGACTAAGGA GCTGCATGAA 
CTTGTGGTGG TGGCCAAGGA TCATGGTGAA CAGCCGCTGG AGACAACCGC CTTTGTTTCC 
ATCAGAGTCA CAGATGTAAA TGACAATCAA CCGACTATAA ATGTGATCTT CCTGAGTGAC 
GATGCCTCTC CAAAAATATC GGAGTCGGCC CAACCGGGCG AATTCGTGGC CAGAATTTCG 
GTGCATGACC CCGATTCGAA AACGGAGTAT GCCAATGTGA ATGTGACACT CAATGGAGGA 
GATGGTCACT TCGCGTTGAC CACCAGGGAT AACAGTATTT ATTTGGTTAT AGTTCACCTG 
CCACTGGACA GGGAGATAGT TTCAAATTAT ACCCTGAGTG TTGTAGCCAC CGATAAGGGA 
ACTCCGCCCC TGCACGCCTC CAAATCGATC TTCCTAAGGA TTACCGATGT CAATGACAAT 
CCACCGGAGT TCGAGCAGGA CCTCTACCAC GCCAACGTCA TGGAGGTGGC CGATCCGGGT 
ACATCGGTAC TTCAAGTTCT AGCCCACGAT CGAGATGAGG GCCTAAACTC GGCTTTGACA 
TACTCGCTGG CCGAAACGCC TGAAACCCAT GCCCAGTGGT TTCAGATTGA CCCACAAACG 
GGCCTGATCA CCACCCGATC GCATATCGAT TGCGAAACGG AGCCGGTGCC GCAGCTAACG 
GTTGTCGCTA GGGACGGAGG TGTTCCGCCC CTTTCCTCCA CAGCCACGGT CCTTGTTACC 
ATTCACGATG TAAACGACAA CGAACCGATC TTCGACCAGA GCTTCTACAA CGTATCCGTC 
GCTGAAAACG AACCCGTGGG CAGGTGCATA CTCAAGGTCT CTGCAAGTGA TCCAGATTGC 
GGAGTAAATG CCATGGTGAA CTACACCATC GGCGAGGGCT TCAAGCACCT CACCGAATTC 
GAGGTGCGCT CCGCTTCTGG CGAGATTTGC ATAGCTGGCG AACTTGACTT CGAGCGGAGG 
AGTAGCTATG AATTTCCCGT ACTGGCAACC GATCGCGGGG GTCTGAGCAC TACTGCCATG 
ATTAAAATGC AATTGACGGA CGTGAACGAC AATCGTCCGG TTTTCTATCC CAGGGAATAT 
AAAGTATCGC TCAGGGAATC CCCGAAAGCC TCCTCACAAG CTTCGAGTAC TCCGATTGTC 
GCTGTGGTGG CCACAGATCC GGATTACGGA AACTTTGGCC AGGTCTCATA TAGGATTGTG 
GCCGGTAACG AGGCGGGCAT CTTCCGGATT GATCGATCCA CTGGTGAAAT ATTCGTGGTC 
CGTCCGGATA TGCTATCCGT TCGTACCCAA CCCATGCACA TGCTAAATAT ATCGGCGACC 
GATGGCGGTA ATCTAAGGAG CAATGCCGAC GCCGTGGTCT TCTTGAGCAT CATCGACGCC 
ATGCAGCGAC CGCCGATCTT CGAAAAGGCC AGATACAATT ACTATGTGAA GGAGGACATT 
CCGAGGGGCA CGGTTGTGGG TTCTGTGATA GCTGCGAGTG GAGATGTCGC CCATCGAAGT 
CCGGTTAGGT ATTCGATATA CTCGGGCGAT CCCGATGGCT ATTTTAGCAT AGAAACCAAC 
TCTGGAAACA TACGTATAGC TAAGCCCCTG GATCATGAAG CGAAGTCCCA GGTTCTACTC 
AACATCCAAG CCACCTTGGG AGAACCTCCG GTTTATGGAC ACACACAGGT TAATATCGAA 
GTGGAGGATG TCAACGATAA TGCACCTGAG TTCGAGGCCA GTATGGTTAG GATTTCCGTA 
CCGGAAAGTG CAGAGCTTGG AGCTCCACTA TATGCTGCAC ATGCCCACGA TAAAGATTCG 
GGAAGCAGTG GTCAAGTTAC ATATAGCCTG GTCAAGGAAT CGGGGAAAGG ACTCTTTGCC 
ATCGACGCGA GATCTGGCCA CTTAATTTTG TCCCAACACC TAGATTACGA GAGCTCCCAA 
CGACACACCC TAATTGTAAC CGCCACGGAT GGTGGAGTTC CCAGCCTTTC GACGAACTTA 
ACCATCTTGG TGGATGTACA GGATGTCAAC GATAACCCTC CAGTTTTTGA AAAGGACGAG 
TATTCTGTGA ATGTGTCCGA ATCGCGATCG ATCAATGCTC AGATTATTCA AGTTAATGCC 
AGCGATCTGG ACACGGGCAA CAATGCCAGG ATCACCTATA GGATTGTCGA TGCCGGAGTG 
GACAACGTCA CGAACTCCAT TAGTAGCTCA GATGTGTCGC AACATTTTGG CATATTTCCA 
AACTCCGGCT GGATATATTT GCGTGCTCCT CTGGATCGAG AGACCAGGGA TCGCTATCAA 
CTGACGGTTT TGGCCACGGA CAATGGCACT CCCGCTGCTC ACGCAAAAAC ACGTGTCATA 
GTTCGTGTTC TTGATGCCAA CGATAATGAT CCCAAGTTCC AGAAATCTAA ATACGAATTT 
AGGATAGAGG AGAACCTCAG GAGAGGCTCC GTTGTGGGAG TGGTGACCGC CAGTGATCTG 
GATCTGGGTG AGAATGCCGC CATAAGATAC AGCCTGCTGC CGATCAACTC CAGCTTCCAG 
GTGCATCCCG TCACAGGAGA AATTTCCACC CGAGAGCCTC TGGATCGAGA GCTCCGGGAG 
CTGTACGACC TTGTAGTGGA GGCCCGTGAT CAAGGAACTC CAGTGAGAAG TGCTCGCGTC 
CCTGTACGCA TCCACGTAAG CGATGTCAAC GATAACGCAC CCGAGATCGC AGATCCGCAG 
GAGGATGTGG TGAGCGTAAG AGAGGAGCAA CCACCCGGAA CTGAAGTGGT GCGAGTTCGA 
GCCGTCGATC GGGACCACGG GCAGAATGCC TCCATTACAT ATTCAATTGT CAAGGGACGG 
GACTCTGATG GGCACGGACT GTTCAGCATC GATCCCACCA GCGGAGTGAT CCGAACCCGA 
GTGGTTTTGG ATCACGAGGA AAGGAGCATC TATCGCCTGG GTGTGGCCGC CTCCGACGGC 
GGCAATCCGC CCAGGGAGAC GGTACGGATG TTGCGGGTTG AAGTTTTGGA TCTGAACGAC 
AACCGCCCAA CATTTACCAG TTCCAGCTTG GTCTTCAGGG TACGCGAGGA TGCCGCCCTT 
GGCCATGTGG TCGGTTCCAT CAGTCCCATC GAACGTCCGG CGGATGTCGT CAGAAATAGC 
GTGGAGGAGT CCTTCGAAGA TCTGCGCGTC ACCTACACTC TAAATCCCTT GACCAAGGAC 
CTGATCGAGG CTGCCTTCGA CATCGATCGG CATTCGGGAA ACCTGGTCGT GGCTCGTCTC 
TTGGACAGAG AGGTTCAATC CGAATTTAGG TTGGAAATTA GGGCTTTAGA TACCACTGCT 
AGTAATAACC CGCAGAGCAG CGCCATCACG GTTAAGATCG AGGTGGCCGA TGTCAATGAT 
AATGCCCCTG AATGGCCACA GGATCCCATC GACCTTCAGG TGAGCGAAGC CACTCCAGTT 
GGCACAATAA TACACAACTT TACGGCCACG GATGCGGACA CGGGAACCAA TGGGGACCTA 
CAGTATCGAT TGATCCGATA CTTCCCCCAA CTCAATGAAA GCCAAGAGCA GGCAATGAGT 
CTTTTTAGAA TGGATTCCCT GACGGGAGCA CTCAGTCTGC AGGCTCCTTT GGACTTCGAG 
GCTGTGCAGG AGTACCTTCT TATTGTCCAG GCACTGGATC AATCCTCGAA TGTAACAGAA 
AGACTGCAGA CTTCGGTGAC CGTGAGATTG CGAATTCTAG ATGCCAACGA CCATGCCCCG 
CACTTTGTTT CACCCAATTC AAGTGGTGGC AAAACGGCAT CTCTTTTCAT AAGTGATGCA 
ACTAGAATCG GAGAGGTCGT GGCGCACATT GTGGCCGTTG ATGAAGATTC CGGGGACAAT 
GGACAACTTA CCTACGAAAT CACGGGGGGC AATGGGGAAG GCCGCTTTAG AATCAATTCC 
CAAACAGGAA TAATTGAGCT GGTTAAGTCC CTGCCACCGG CCACCGAGGA CGTGGAAAAA 
GGTGGACGGT TTAATCTAAT CATTGGTGCA AAAGATCATG GTCAACCGGA GCCCAAGAAA 
AGCTCTCTAA ATCTGCACCT TATCGTTCAA GGCAGCCACA ATAATCCACC CCGATTTCTG 
CAGGCTGTTT ATCGAGCCAC AATTTTGGAG AACGTTCCTA GCGGAAGTTT CGTCCTGCAA 
GTTACGGCAA AATCACTACA TGGTGCAGAA AATGCTAATT TAAGCTATGA AATTCCTGCT 
GGCGTGGCAA ATGATCTGTT CCACGTGGAC TGGCAGAGGG GCATCATTAC CACACGGGGA 
CAGTTTGACC GGGAGTCCCA GGCAAGTTAT GTCCTGCCCG TCTACGTACG GGATGCCAAC 
CGGCAGTCGA CTTTGTCCTC TTCAGCGGTG AGAAAGCAAC GCTCGTCGGA CAGCATCGGA 
GATACATCGA ATGGCCAGCA CTTTGATGTG GCCACAATTT ATATAACGGT AGGAGATGTC 
AACGACAACT CCCCGGAATT CCGACCAGGG TCATGTTATG GTCTATCAGT CCCAGAGAAC 
AGTGAACCCG GTGTAATTCA CACTGTGGTG GCCAGCGATC TGGATGAAGG TCCGAATGCG 
GATCTTATTT ACAGTATTAC GGGCGGCAAT TTGGGCAATA AGTTCAGCAT TGATTCTAGT 
TCAGGAGAGC TAAGTGCCCG ACCCTTGGAT AGAGAACAGC ACTCTCGGTA CACCCTGCAG 
ATTCAGGCCT CCGATCGGGG GCAACCGAAG AGCCGTCAAG GACACTGCAA CATCACCATA 
TTCGTCGAGG ATCAGAACGA CAATGCCCCG AGATTCAAGC TGAGCAAATA TACTGGCAGT 
GTGCAGGAGG ACGCACCACT GGGCACCAGT GTGGTGCAGA TCAGTGCGGT GGATGCGGAT 
TTGGGCGTCA ACGCCAGACT CGTTTACTCG CTGGCCAATG AAACGCAATG GCAGTTTGCC 
ATCGACGGCC AGAGCGGATT AATCACCACT GTGGGAAAAC TGGATCGCGA GCTGCAGGCG 
AGCTACAACT TCATGGTATT GGCCACGGAC GGAGGACGTT ATGAAGTGAG ATCAGCTACG 
GTTCCGGTGC AGATCAACGT GCTGGATATT AATGACAACA GACCCATTTT CGAGCGATAT 
CCCTATATAG GCCAGGTCCC AGCTCTCATC CAACCTGGCC AGACTTTGCT GAAGGTTCAA 
GCCCTAGATG CAGATTTGGG TGCGAATGCC GAGATTGTGT ATTCCCTCAA TGCTGAAAAT 
TCAGCAGTAA GTGCCAAGTT TAGAATAAAT CCAAGCACTG GTGCCTTGAG TGCCTCTCAG 
AGTTTGGCCA GTGAATCTGG GAAGCTTTTA CACCTGGAGG TCGTTGCCAG GGATAAGGGA 
AATCCGCCTC AATCCAGCCT GGGCCTCATA GAACTTCTAA TAGGGGAAGC ACCGCAAGGA 
ACTCCAGTTC TTAGGTTCCA GAATGAGACC TATCGAGTAA TGCTCAAGGA GAATTCTCCA 
TCGGGTACAA GACTTCTTCA AGTGGTTGCA CTGCGCAGTG ATGGCCGCCG GCAAAAGGTG 
CAATTCTCAT TTGGGGCTGG TAATGAAGAT GGGATTTTGT CCTTAGACTC ACTCTCAGGT 
GAAATAAGGG TGAATAAGCC CCATTTACTT GATTACGACC GCTTCTCCAC ACCCTCAATG 
TCAGCCTTAA GTCGTGGCAG AGCTCTGCAT TATGAGGAGG AAATCGATGA ATCATCAGAG 
GAGGATCCAA ATAATAGCAC CCGATCGCAA AGGGCCTTGA CGTCCTCATC TTTCGCCCTG 
ACCAACAGCC AACCCAATGA AATACGTGTG GTCTTAGTTG CGCGCACCGC AGATGCTCCA 
TTCCTGGCCA GCTATGCGGA ACTAGTGATT GAGCTTGAAG ACGAGAACGA CAATAGCCCA 
AAGTTTTCGC AAAAGCAATT TGTGGCAACG GTTTCAGAAG GAAACAACAA GGGAACCTTT 
GTGGCACAAG TTCATGCATT CGATTCGGAT GCGGGCTCCA ACGCCCGTCT GCGATATCAC 
ATCGTTGATG GCAACCACGA CAATGCATTC GTTATTGAAC CCGCCTTCAG TGGCATAGTG 
AGGACCAACA TAGTGCTGGA TCGGGAGATT CGCGATATAT ATAAGCTCAA GATAATTGCA 
ACCGATGAGG GAGTGCCTCA AATGACTGGC ACGGCCACAA TAAGAGTGCA GATCGTCGAT 
GTGAATGACA ACCAGCCCAC TTTTCCGCCA AACAATCTCG TCACAGTCAG TGAAGCTACT 
GAACTTGGAG CCGTAATAAC CTCTATTTCA GCCAACGATG TGGACACTTA CCCCGCCCTG 
ACCTACCGCC TTGGCGCAGA GTCTACCGTG GATATAGAAA ACATGTCCAT CTTTGCCTTG 
GATCGATATA GCGGGAAGCT CGTTCTAAAA CGTCGGCTGG ACTATGAGCT CCAGCAGGAG 
TATGAACTCG ACGTGATTGC TTCGGATGCA GCCCATGAAG CTCGCACTGT TCTAACTGTG 
CGAGTGAACG ATGAAAACGA CAATGCACCA GTATTCCTGG CCCAACAGCC TCCGGCTTAC 
TTTGCCATTT TGCCAGCTAT ATCCGAGATC AGTGAGAGTC TTTCCGTGGA CTTCGATCTC 
CTAACTGTCA ACGCCACTGA CGCAGACTCG GAGGGTAATA ACTCAAAGGT TATTTATATC 
ATTGAACCTG CCCAGGAGGG CTTCAGTGTG CACCCATCAA ATGGTGTTGT GTCTGTTAAT 
ATGTCTAGAT TACAGCCGGC CGTATCCTCT AGCGGGGATT ATTTTGTAAG AATTATAGCC 
AAGGACGCAG GAAAACCAGC TTTGAAAAGC TCAACACTTT TGAGAGTACA GGCAAATGAC 
AACGGTTCCG GACGTTCGCA ATTCCTACAG AACCAATATA GGGCTCAGAT AAGCGAGGCA 
GCTCCTTTGG GATCGGTTGT TCTCCAGCTT GGACAAGATG CTCTGGATCA ATCCTTAGCT 
ATCATTGCTG GAAATGAAGA ATCCGCTTTT GAGTTGCTCC AATCCAAAGC AATTGTCTTG 
GTCAAGCCCT TGGATAGAGA ACGCAATGAT CTCTACAAAC TGCGATTGGT TTTAAGCCAT 
CCACATGGAC CTCCACTTAT TTCAAGCTTA AATTCCTCGT CTGGAATCTC AGTAATCATA 
ACAATCCTGG ATGCCAACGA TAACTTCCCA ATTTTCGATC GAAGCGCTAA ATATGAGGCT 
GAGATCAGCG AACTTGCTCC TCTGCGGTAC TCCATAGCGC AGTTGCAGGC CATTGATGCC 
GATCAGGAAA ACACCCCAAA CTCAGAAGTG GTATACGACA TAACCTCTGG CAATGATGAG 
CATATGTTTA CGATAGACTT GGTCACCGGC GTTCTCTTTG TGAACAATCG CTTGGACTAC 
GATTCGGGGG CCAAGAGTTA CGAGCTAATA ATCCGAGCCT GCGATAGTCA TCACCAAAGA 
CCACTTTGCA GCTTGCAACC CTTTCGATTG GAACTCCATG ACGAGAACGA CAACGAACCC 
AAGTTCCCGC TAACTGAATA TGTTCACTTC CTGGCTGAAA ACGAGCCGGT TGGCAGCTCC 
GTATTCCGTG CTCATGCCAG TGATTTGGAT AAGGGACCCT TCGGGCAATT AAACTACAGC 
ATAGGTCCTG CTCCCAGCGA CGAGAGCTCT TGGAAAATGT TCCGAGTTGA TTCGGAAAGT 
GGTCTCGTTA CATCCGCCTT CGTATTCGAC TATGAGCAAA GACAGCGCTA TGACATGGAG 
CTGCTCGCCT CCGATATGGG CGGTAAGAAG GCCAGTGTGG CTGTGCGCGT AGAGATTGAG 
TCGAGGGATG AGTTCACCCC GCAGTTCACA GAGCGCACCT ATAGGTTTGT GCTGCCAGCC 
GCCGTGGCCC TACCCCAAGG CTACGTGGTG GGTCAAGTGA CTGCCACTGA TAGTGACAGT 
GGCCCGGACG GACGAGTGGT CTATCAACTA AGTGCCCCAC ACTCGCATTT CAAGGTAAAC 
CGTAGCAGCG GGGCTGTTCT AATCAAGAGG AAACTCAAAC TGGATGGCGA CGGAGACGGT 
AATCTGTACA TGGATGGTCG AGATATCAGT CTAGTTATCT CAGCTTCCTC GGGGCGTCAC 
AACTCCTTAT CCAGCATGGC TGTTGTCGAG ATTGCCTTGG ATCCTTTGGC ACATCCAGGC 
ACAAACCTGG CCAGTGCAGG TGGTTCGTCA AGTGGTTCCA TTGGCGATTG GGCAATTGGT 
CTACTGGTAG CTTTTCTTCT AGTCCTCTGC GCTGCCGCCG GAATCTTTCT ATTCATCCAT 
ATGAGAAGCA GAAAACCGCG TAATGCGGTC AAGCCTCATC TTGCCACGGA CAATGCTGGT 
GTGGGCAATA CCAACAGCTA TGTGGACCCC AGTGCTTTCG ACACGATCCC TATAAGAGGA 
AGCATATCGG GCGGTGCTGC AGGAGCGGCA TCTGGTCAAT TCGCTCCTCC GAAGTACGAC 
GAGATTCCTC CGTTTGGAGC TCATGCTGGC AGCTCCGGAG CAGCTACAAC CTCCGAGTTG 
TCGGGCTCCG AACAGTCCGG TTCCAGTGGC CGAGGCTCTG CTGAGGACGA TGGGGAGGAT 
GAGGAGATTC GTATGATCAA CGAGGGACCA CTACATCACC GCAATGGTGG AGCTGGGGCA 
GGCAGCGATG ATGGCCGAAT TTCGGATATA TCTGTGCAAA ACACCCAGGA ATACTTGGCT 
CGCCTGGGCA TCGTAGATCA CGATCCTAGT GGCGCCGGAG GAGGAGCTTC CAGTATGGCT 
GGTTCAAGTC ACCCAATGCA CTTGTATCAC GATGATGATG CCACAGCACG GTCGGATATT 
ACGAACCTCA TCTATGCCAA ACTTAATGAT GTGACAGGAG CTGGTTCAGA GATAGGAAGC 
AGTGCAGACG ACGCGGGAAC CACTGCGGGC TCCATCGGAA CCATCGGAAC GGCTATAACC 
CATGGCCATG GGGTGATGAG CAGTTACGGA GAAGTTCCTG TTCCAGTACC GGTTGTGGTT 
GGCGGTAGTA ATGTGGGTGG TTCCCTGAGC TCCATAGTTC ACAGTGAGGA GGAATTGACT 
GGCAGCTACA ACTGGGATTA CCTATTGGAT TGGGGACCGC AATATCAGCC ACTTGCGCAT 
GTGTTTTCCG AAATAGCGAG ACTGAAAGAT GACACCTTAT CGGAGCACAG TGGAAGTGGG 
GCTTCCTCGT CCGCCAAGAG CAAGCACTCT TCCTCACATT CCTCAGCAGG AGCAGGAAGC 
GTGGTGCTCA AACCACCACC CTCAGCACCA CCGACTCACA TACCACCCCC CCTGCTAACC 
AATGTGGCAC CAAGGGCCAT TAACCTGCCT ATGCGCTTGC CACCCCATTT GAGCCTGGCC 
CCCGCCCATT TGCCCAGATC GCCCATTGGT CATGAGGCGA GTGGCAGTTT CAGCACATCA 
TCAGCAATGT CACCTTCGTT CTCGCCCTCT CTATCGCCGT TGGCAACAAG ATCACCATCC 
ATTTCTCCTC TGGGAGCGGG ACCACCCACG CACCTGCCTC ATGTCTCGTT GCCACGCCAC 
GGTCATGCCC CTCAGCCAAG TCAGAGGGGA AATGTGGGGA CACGGATGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285551.1
CDS	811..11481
Translation	MKATLDSVVASETMTRVTFTGNHESKDFAIFHRCRRRSNIKSLYSPPALHPSHMLRSSLLILLAIVLLGSSQAASHDQERERKLEVFEGVAVDYQIGYIGDFGGIDSGPPYIIVAEAGVETDLAIDRATGEIRTKVKLDRETRASYSLVAIPLSGRNIRVLVTVKDENDNAPTFPQTSMHIEFPENTPREVKRTLLPARDLDLEPYNTQRYNIVSGNVNDAFRLSSHRERDGVLYLDLQISGFLDRETTPGYSLLIEALDGGTPPLRGFMTVNITIQDVNDNQPIFNQSRYFATVPENATVGTSVLQVYASDTDADENGLVEYAINRRQSDKEQMFRIDPRTGAIYINKALDFETKELHELVVVAKDHGEQPLETTAFVSIRVTDVNDNQPTINVIFLSDDASPKISESAQPGEFVARISVHDPDSKTEYANVNVTLNGGDGHFALTTRDNSIYLVIVHLPLDREIVSNYTLSVVATDKGTPPLHASKSIFLRITDVNDNPPEFEQDLYHANVMEVADPGTSVLQVLAHDRDEGLNSALTYSLAETPETHAQWFQIDPQTGLITTRSHIDCETEPVPQLTVVARDGGVPPLSSTATVLVTIHDVNDNEPIFDQSFYNVSVAENEPVGRCILKVSASDPDCGVNAMVNYTIGEGFKHLTEFEVRSASGEICIAGELDFERRSSYEFPVLATDRGGLSTTAMIKMQLTDVNDNRPVFYPREYKVSLRESPKASSQASSTPIVAVVATDPDYGNFGQVSYRIVAGNEAGIFRIDRSTGEIFVVRPDMLSVRTQPMHMLNISATDGGNLRSNADAVVFLSIIDAMQRPPIFEKARYNYYVKEDIPRGTVVGSVIAASGDVAHRSPVRYSIYSGDPDGYFSIETNSGNIRIAKPLDHEAKSQVLLNIQATLGEPPVYGHTQVNIEVEDVNDNAPEFEASMVRISVPESAELGAPLYAAHAHDKDSGSSGQVTYSLVKESGKGLFAIDARSGHLILSQHLDYESSQRHTLIVTATDGGVPSLSTNLTILVDVQDVNDNPPVFEKDEYSVNVSESRSINAQIIQVNASDLDTGNNARITYRIVDAGVDNVTNSISSSDVSQHFGIFPNSGWIYLRAPLDRETRDRYQLTVLATDNGTPAAHAKTRVIVRVLDANDNDPKFQKSKYEFRIEENLRRGSVVGVVTASDLDLGENAAIRYSLLPINSSFQVHPVTGEISTREPLDRELRELYDLVVEARDQGTPVRSARVPVRIHVSDVNDNAPEIADPQEDVVSVREEQPPGTEVVRVRAVDRDHGQNASITYSIVKGRDSDGHGLFSIDPTSGVIRTRVVLDHEERSIYRLGVAASDGGNPPRETVRMLRVEVLDLNDNRPTFTSSSLVFRVREDAALGHVVGSISPIERPADVVRNSVEESFEDLRVTYTLNPLTKDLIEAAFDIDRHSGNLVVARLLDREVQSEFRLEIRALDTTASNNPQSSAITVKIEVADVNDNAPEWPQDPIDLQVSEATPVGTIIHNFTATDADTGTNGDLQYRLIRYFPQLNESQEQAMSLFRMDSLTGALSLQAPLDFEAVQEYLLIVQALDQSSNVTERLQTSVTVRLRILDANDHAPHFVSPNSSGGKTASLFISDATRIGEVVAHIVAVDEDSGDNGQLTYEITGGNGEGRFRINSQTGIIELVKSLPPATEDVEKGGRFNLIIGAKDHGQPEPKKSSLNLHLIVQGSHNNPPRFLQAVYRATILENVPSGSFVLQVTAKSLHGAENANLSYEIPAGVANDLFHVDWQRGIITTRGQFDRESQASYVLPVYVRDANRQSTLSSSAVRKQRSSDSIGDTSNGQHFDVATIYITVGDVNDNSPEFRPGSCYGLSVPENSEPGVIHTVVASDLDEGPNADLIYSITGGNLGNKFSIDSSSGELSARPLDREQHSRYTLQIQASDRGQPKSRQGHCNITIFVEDQNDNAPRFKLSKYTGSVQEDAPLGTSVVQISAVDADLGVNARLVYSLANETQWQFAIDGQSGLITTVGKLDRELQASYNFMVLATDGGRYEVRSATVPVQINVLDINDNRPIFERYPYIGQVPALIQPGQTLLKVQALDADLGANAEIVYSLNAENSAVSAKFRINPSTGALSASQSLASESGKLLHLEVVARDKGNPPQSSLGLIELLIGEAPQGTPVLRFQNETYRVMLKENSPSGTRLLQVVALRSDGRRQKVQFSFGAGNEDGILSLDSLSGEIRVNKPHLLDYDRFSTPSMSALSRGRALHYEEEIDESSEEDPNNSTRSQRALTSSSFALTNSQPNEIRVVLVARTADAPFLASYAELVIELEDENDNSPKFSQKQFVATVSEGNNKGTFVAQVHAFDSDAGSNARLRYHIVDGNHDNAFVIEPAFSGIVRTNIVLDREIRDIYKLKIIATDEGVPQMTGTATIRVQIVDVNDNQPTFPPNNLVTVSEATELGAVITSISANDVDTYPALTYRLGAESTVDIENMSIFALDRYSGKLVLKRRLDYELQQEYELDVIASDAAHEARTVLTVRVNDENDNAPVFLAQQPPAYFAILPAISEISESLSVDFDLLTVNATDADSEGNNSKVIYIIEPAQEGFSVHPSNGVVSVNMSRLQPAVSSSGDYFVRIIAKDAGKPALKSSTLLRVQANDNGSGRSQFLQNQYRAQISEAAPLGSVVLQLGQDALDQSLAIIAGNEESAFELLQSKAIVLVKPLDRERNDLYKLRLVLSHPHGPPLISSLNSSSGISVIITILDANDNFPIFDRSAKYEAEISELAPLRYSIAQLQAIDADQENTPNSEVVYDITSGNDEHMFTIDLVTGVLFVNNRLDYDSGAKSYELIIRACDSHHQRPLCSLQPFRLELHDENDNEPKFPLTEYVHFLAENEPVGSSVFRAHASDLDKGPFGQLNYSIGPAPSDESSWKMFRVDSESGLVTSAFVFDYEQRQRYDMELLASDMGGKKASVAVRVEIESRDEFTPQFTERTYRFVLPAAVALPQGYVVGQVTATDSDSGPDGRVVYQLSAPHSHFKVNRSSGAVLIKRKLKLDGDGDGNLYMDGRDISLVISASSGRHNSLSSMAVVEIALDPLAHPGTNLASAGGSSSGSIGDWAIGLLVAFLLVLCAAAGIFLFIHMRSRKPRNAVKPHLATDNAGVGNTNSYVDPSAFDTIPIRGSISGGAAGAASGQFAPPKYDEIPPFGAHAGSSGAATTSELSGSEQSGSSGRGSAEDDGEDEEIRMINEGPLHHRNGGAGAGSDDGRISDISVQNTQEYLARLGIVDHDPSGAGGGASSMAGSSHPMHLYHDDDATARSDITNLIYAKLNDVTGAGSEIGSSADDAGTTAGSIGTIGTAITHGHGVMSSYGEVPVPVPVVVGGSNVGGSLSSIVHSEEELTGSYNWDYLLDWGPQYQPLAHVFSEIARLKDDTLSEHSGSGASSSAKSKHSSSHSSAGAGSVVLKPPPSAPPTHIPPPLLTNVAPRAINLPMRLPPHLSLAPAHLPRSPIGHEASGSFSTSSAMSPSFSPSLSPLATRSPSISPLGAGPPTHLPHVSLPRHGHAPQPSQRGNVGTRM

Target ORF information:

RefSeq Version	NM_001298622.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster dachsous (ds), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298622.1

ORF Insert Sequence:

ATGAAAGCCA CGTTGGATTC GGTAGTCGCC AGCGAAACGA TGACGAGGGT AACATTCACT 
GGGAACCACG AGTCGAAGGA CTTTGCAATA TTCCATCGAT GCCGGAGACG ATCCAACATC 
AAATCGCTAT ATTCGCCACC TGCCCTGCAT CCCTCGCACA TGCTGCGAAG TAGCTTACTA 
ATTCTACTGG CAATCGTTCT CCTGGGATCT TCGCAGGCTG CCTCACACGA TCAGGAGAGG 
GAGCGAAAGC TGGAAGTCTT CGAAGGCGTT GCCGTAGACT ATCAAATCGG TTATATAGGA 
GACTTCGGCG GAATCGATAG TGGTCCGCCA TACATTATTG TGGCGGAGGC AGGAGTAGAA 
ACAGATCTCG CCATAGATCG GGCCACTGGA GAGATCCGCA CAAAGGTCAA ACTGGACAGA 
GAAACTCGAG CTTCTTATAG TCTAGTGGCT ATTCCCCTGA GTGGTCGTAA TATTCGAGTG 
CTGGTCACAG TTAAAGATGA GAATGACAAT GCCCCCACGT TTCCACAAAC CTCGATGCAC 
ATAGAGTTTC CAGAAAATAC CCCAAGGGAG GTAAAGAGAA CTCTTCTTCC AGCAAGGGAT 
CTGGATCTGG AACCCTACAA CACTCAGAGA TATAATATCG TGTCGGGAAA CGTAAATGAC 
GCCTTTAGAT TGTCGTCCCA CCGAGAAAGG GACGGTGTCC TGTACTTGGA TCTGCAGATC 
AGTGGTTTTT TAGATAGAGA GACCACGCCA GGCTACAGCC TTTTAATTGA AGCTTTAGAT 
GGAGGAACAC CCCCGTTGCG TGGATTTATG ACTGTTAATA TTACCATTCA GGATGTAAAT 
GACAATCAAC CGATCTTCAA TCAGAGCAGG TACTTCGCAA CGGTGCCAGA GAATGCCACT 
GTGGGCACCA GTGTCCTGCA GGTGTATGCA TCAGACACGG ATGCCGATGA GAATGGTTTG 
GTGGAATACG CCATCAACAG AAGACAGAGC GACAAGGAGC AAATGTTTCG CATAGATCCA 
AGAACAGGAG CGATTTACAT CAATAAAGCA CTGGATTTCG AGACTAAGGA GCTGCATGAA 
CTTGTGGTGG TGGCCAAGGA TCATGGTGAA CAGCCGCTGG AGACAACCGC CTTTGTTTCC 
ATCAGAGTCA CAGATGTAAA TGACAATCAA CCGACTATAA ATGTGATCTT CCTGAGTGAC 
GATGCCTCTC CAAAAATATC GGAGTCGGCC CAACCGGGCG AATTCGTGGC CAGAATTTCG 
GTGCATGACC CCGATTCGAA AACGGAGTAT GCCAATGTGA ATGTGACACT CAATGGAGGA 
GATGGTCACT TCGCGTTGAC CACCAGGGAT AACAGTATTT ATTTGGTTAT AGTTCACCTG 
CCACTGGACA GGGAGATAGT TTCAAATTAT ACCCTGAGTG TTGTAGCCAC CGATAAGGGA 
ACTCCGCCCC TGCACGCCTC CAAATCGATC TTCCTAAGGA TTACCGATGT CAATGACAAT 
CCACCGGAGT TCGAGCAGGA CCTCTACCAC GCCAACGTCA TGGAGGTGGC CGATCCGGGT 
ACATCGGTAC TTCAAGTTCT AGCCCACGAT CGAGATGAGG GCCTAAACTC GGCTTTGACA 
TACTCGCTGG CCGAAACGCC TGAAACCCAT GCCCAGTGGT TTCAGATTGA CCCACAAACG 
GGCCTGATCA CCACCCGATC GCATATCGAT TGCGAAACGG AGCCGGTGCC GCAGCTAACG 
GTTGTCGCTA GGGACGGAGG TGTTCCGCCC CTTTCCTCCA CAGCCACGGT CCTTGTTACC 
ATTCACGATG TAAACGACAA CGAACCGATC TTCGACCAGA GCTTCTACAA CGTATCCGTC 
GCTGAAAACG AACCCGTGGG CAGGTGCATA CTCAAGGTCT CTGCAAGTGA TCCAGATTGC 
GGAGTAAATG CCATGGTGAA CTACACCATC GGCGAGGGCT TCAAGCACCT CACCGAATTC 
GAGGTGCGCT CCGCTTCTGG CGAGATTTGC ATAGCTGGCG AACTTGACTT CGAGCGGAGG 
AGTAGCTATG AATTTCCCGT ACTGGCAACC GATCGCGGGG GTCTGAGCAC TACTGCCATG 
ATTAAAATGC AATTGACGGA CGTGAACGAC AATCGTCCGG TTTTCTATCC CAGGGAATAT 
AAAGTATCGC TCAGGGAATC CCCGAAAGCC TCCTCACAAG CTTCGAGTAC TCCGATTGTC 
GCTGTGGTGG CCACAGATCC GGATTACGGA AACTTTGGCC AGGTCTCATA TAGGATTGTG 
GCCGGTAACG AGGCGGGCAT CTTCCGGATT GATCGATCCA CTGGTGAAAT ATTCGTGGTC 
CGTCCGGATA TGCTATCCGT TCGTACCCAA CCCATGCACA TGCTAAATAT ATCGGCGACC 
GATGGCGGTA ATCTAAGGAG CAATGCCGAC GCCGTGGTCT TCTTGAGCAT CATCGACGCC 
ATGCAGCGAC CGCCGATCTT CGAAAAGGCC AGATACAATT ACTATGTGAA GGAGGACATT 
CCGAGGGGCA CGGTTGTGGG TTCTGTGATA GCTGCGAGTG GAGATGTCGC CCATCGAAGT 
CCGGTTAGGT ATTCGATATA CTCGGGCGAT CCCGATGGCT ATTTTAGCAT AGAAACCAAC 
TCTGGAAACA TACGTATAGC TAAGCCCCTG GATCATGAAG CGAAGTCCCA GGTTCTACTC 
AACATCCAAG CCACCTTGGG AGAACCTCCG GTTTATGGAC ACACACAGGT TAATATCGAA 
GTGGAGGATG TCAACGATAA TGCACCTGAG TTCGAGGCCA GTATGGTTAG GATTTCCGTA 
CCGGAAAGTG CAGAGCTTGG AGCTCCACTA TATGCTGCAC ATGCCCACGA TAAAGATTCG 
GGAAGCAGTG GTCAAGTTAC ATATAGCCTG GTCAAGGAAT CGGGGAAAGG ACTCTTTGCC 
ATCGACGCGA GATCTGGCCA CTTAATTTTG TCCCAACACC TAGATTACGA GAGCTCCCAA 
CGACACACCC TAATTGTAAC CGCCACGGAT GGTGGAGTTC CCAGCCTTTC GACGAACTTA 
ACCATCTTGG TGGATGTACA GGATGTCAAC GATAACCCTC CAGTTTTTGA AAAGGACGAG 
TATTCTGTGA ATGTGTCCGA ATCGCGATCG ATCAATGCTC AGATTATTCA AGTTAATGCC 
AGCGATCTGG ACACGGGCAA CAATGCCAGG ATCACCTATA GGATTGTCGA TGCCGGAGTG 
GACAACGTCA CGAACTCCAT TAGTAGCTCA GATGTGTCGC AACATTTTGG CATATTTCCA 
AACTCCGGCT GGATATATTT GCGTGCTCCT CTGGATCGAG AGACCAGGGA TCGCTATCAA 
CTGACGGTTT TGGCCACGGA CAATGGCACT CCCGCTGCTC ACGCAAAAAC ACGTGTCATA 
GTTCGTGTTC TTGATGCCAA CGATAATGAT CCCAAGTTCC AGAAATCTAA ATACGAATTT 
AGGATAGAGG AGAACCTCAG GAGAGGCTCC GTTGTGGGAG TGGTGACCGC CAGTGATCTG 
GATCTGGGTG AGAATGCCGC CATAAGATAC AGCCTGCTGC CGATCAACTC CAGCTTCCAG 
GTGCATCCCG TCACAGGAGA AATTTCCACC CGAGAGCCTC TGGATCGAGA GCTCCGGGAG 
CTGTACGACC TTGTAGTGGA GGCCCGTGAT CAAGGAACTC CAGTGAGAAG TGCTCGCGTC 
CCTGTACGCA TCCACGTAAG CGATGTCAAC GATAACGCAC CCGAGATCGC AGATCCGCAG 
GAGGATGTGG TGAGCGTAAG AGAGGAGCAA CCACCCGGAA CTGAAGTGGT GCGAGTTCGA 
GCCGTCGATC GGGACCACGG GCAGAATGCC TCCATTACAT ATTCAATTGT CAAGGGACGG 
GACTCTGATG GGCACGGACT GTTCAGCATC GATCCCACCA GCGGAGTGAT CCGAACCCGA 
GTGGTTTTGG ATCACGAGGA AAGGAGCATC TATCGCCTGG GTGTGGCCGC CTCCGACGGC 
GGCAATCCGC CCAGGGAGAC GGTACGGATG TTGCGGGTTG AAGTTTTGGA TCTGAACGAC 
AACCGCCCAA CATTTACCAG TTCCAGCTTG GTCTTCAGGG TACGCGAGGA TGCCGCCCTT 
GGCCATGTGG TCGGTTCCAT CAGTCCCATC GAACGTCCGG CGGATGTCGT CAGAAATAGC 
GTGGAGGAGT CCTTCGAAGA TCTGCGCGTC ACCTACACTC TAAATCCCTT GACCAAGGAC 
CTGATCGAGG CTGCCTTCGA CATCGATCGG CATTCGGGAA ACCTGGTCGT GGCTCGTCTC 
TTGGACAGAG AGGTTCAATC CGAATTTAGG TTGGAAATTA GGGCTTTAGA TACCACTGCT 
AGTAATAACC CGCAGAGCAG CGCCATCACG GTTAAGATCG AGGTGGCCGA TGTCAATGAT 
AATGCCCCTG AATGGCCACA GGATCCCATC GACCTTCAGG TGAGCGAAGC CACTCCAGTT 
GGCACAATAA TACACAACTT TACGGCCACG GATGCGGACA CGGGAACCAA TGGGGACCTA 
CAGTATCGAT TGATCCGATA CTTCCCCCAA CTCAATGAAA GCCAAGAGCA GGCAATGAGT 
CTTTTTAGAA TGGATTCCCT GACGGGAGCA CTCAGTCTGC AGGCTCCTTT GGACTTCGAG 
GCTGTGCAGG AGTACCTTCT TATTGTCCAG GCACTGGATC AATCCTCGAA TGTAACAGAA 
AGACTGCAGA CTTCGGTGAC CGTGAGATTG CGAATTCTAG ATGCCAACGA CCATGCCCCG 
CACTTTGTTT CACCCAATTC AAGTGGTGGC AAAACGGCAT CTCTTTTCAT AAGTGATGCA 
ACTAGAATCG GAGAGGTCGT GGCGCACATT GTGGCCGTTG ATGAAGATTC CGGGGACAAT 
GGACAACTTA CCTACGAAAT CACGGGGGGC AATGGGGAAG GCCGCTTTAG AATCAATTCC 
CAAACAGGAA TAATTGAGCT GGTTAAGTCC CTGCCACCGG CCACCGAGGA CGTGGAAAAA 
GGTGGACGGT TTAATCTAAT CATTGGTGCA AAAGATCATG GTCAACCGGA GCCCAAGAAA 
AGCTCTCTAA ATCTGCACCT TATCGTTCAA GGCAGCCACA ATAATCCACC CCGATTTCTG 
CAGGCTGTTT ATCGAGCCAC AATTTTGGAG AACGTTCCTA GCGGAAGTTT CGTCCTGCAA 
GTTACGGCAA AATCACTACA TGGTGCAGAA AATGCTAATT TAAGCTATGA AATTCCTGCT 
GGCGTGGCAA ATGATCTGTT CCACGTGGAC TGGCAGAGGG GCATCATTAC CACACGGGGA 
CAGTTTGACC GGGAGTCCCA GGCAAGTTAT GTCCTGCCCG TCTACGTACG GGATGCCAAC 
CGGCAGTCGA CTTTGTCCTC TTCAGCGGTG AGAAAGCAAC GCTCGTCGGA CAGCATCGGA 
GATACATCGA ATGGCCAGCA CTTTGATGTG GCCACAATTT ATATAACGGT AGGAGATGTC 
AACGACAACT CCCCGGAATT CCGACCAGGG TCATGTTATG GTCTATCAGT CCCAGAGAAC 
AGTGAACCCG GTGTAATTCA CACTGTGGTG GCCAGCGATC TGGATGAAGG TCCGAATGCG 
GATCTTATTT ACAGTATTAC GGGCGGCAAT TTGGGCAATA AGTTCAGCAT TGATTCTAGT 
TCAGGAGAGC TAAGTGCCCG ACCCTTGGAT AGAGAACAGC ACTCTCGGTA CACCCTGCAG 
ATTCAGGCCT CCGATCGGGG GCAACCGAAG AGCCGTCAAG GACACTGCAA CATCACCATA 
TTCGTCGAGG ATCAGAACGA CAATGCCCCG AGATTCAAGC TGAGCAAATA TACTGGCAGT 
GTGCAGGAGG ACGCACCACT GGGCACCAGT GTGGTGCAGA TCAGTGCGGT GGATGCGGAT 
TTGGGCGTCA ACGCCAGACT CGTTTACTCG CTGGCCAATG AAACGCAATG GCAGTTTGCC 
ATCGACGGCC AGAGCGGATT AATCACCACT GTGGGAAAAC TGGATCGCGA GCTGCAGGCG 
AGCTACAACT TCATGGTATT GGCCACGGAC GGAGGACGTT ATGAAGTGAG ATCAGCTACG 
GTTCCGGTGC AGATCAACGT GCTGGATATT AATGACAACA GACCCATTTT CGAGCGATAT 
CCCTATATAG GCCAGGTCCC AGCTCTCATC CAACCTGGCC AGACTTTGCT GAAGGTTCAA 
GCCCTAGATG CAGATTTGGG TGCGAATGCC GAGATTGTGT ATTCCCTCAA TGCTGAAAAT 
TCAGCAGTAA GTGCCAAGTT TAGAATAAAT CCAAGCACTG GTGCCTTGAG TGCCTCTCAG 
AGTTTGGCCA GTGAATCTGG GAAGCTTTTA CACCTGGAGG TCGTTGCCAG GGATAAGGGA 
AATCCGCCTC AATCCAGCCT GGGCCTCATA GAACTTCTAA TAGGGGAAGC ACCGCAAGGA 
ACTCCAGTTC TTAGGTTCCA GAATGAGACC TATCGAGTAA TGCTCAAGGA GAATTCTCCA 
TCGGGTACAA GACTTCTTCA AGTGGTTGCA CTGCGCAGTG ATGGCCGCCG GCAAAAGGTG 
CAATTCTCAT TTGGGGCTGG TAATGAAGAT GGGATTTTGT CCTTAGACTC ACTCTCAGGT 
GAAATAAGGG TGAATAAGCC CCATTTACTT GATTACGACC GCTTCTCCAC ACCCTCAATG 
TCAGCCTTAA GTCGTGGCAG AGCTCTGCAT TATGAGGAGG AAATCGATGA ATCATCAGAG 
GAGGATCCAA ATAATAGCAC CCGATCGCAA AGGGCCTTGA CGTCCTCATC TTTCGCCCTG 
ACCAACAGCC AACCCAATGA AATACGTGTG GTCTTAGTTG CGCGCACCGC AGATGCTCCA 
TTCCTGGCCA GCTATGCGGA ACTAGTGATT GAGCTTGAAG ACGAGAACGA CAATAGCCCA 
AAGTTTTCGC AAAAGCAATT TGTGGCAACG GTTTCAGAAG GAAACAACAA GGGAACCTTT 
GTGGCACAAG TTCATGCATT CGATTCGGAT GCGGGCTCCA ACGCCCGTCT GCGATATCAC 
ATCGTTGATG GCAACCACGA CAATGCATTC GTTATTGAAC CCGCCTTCAG TGGCATAGTG 
AGGACCAACA TAGTGCTGGA TCGGGAGATT CGCGATATAT ATAAGCTCAA GATAATTGCA 
ACCGATGAGG GAGTGCCTCA AATGACTGGC ACGGCCACAA TAAGAGTGCA GATCGTCGAT 
GTGAATGACA ACCAGCCCAC TTTTCCGCCA AACAATCTCG TCACAGTCAG TGAAGCTACT 
GAACTTGGAG CCGTAATAAC CTCTATTTCA GCCAACGATG TGGACACTTA CCCCGCCCTG 
ACCTACCGCC TTGGCGCAGA GTCTACCGTG GATATAGAAA ACATGTCCAT CTTTGCCTTG 
GATCGATATA GCGGGAAGCT CGTTCTAAAA CGTCGGCTGG ACTATGAGCT CCAGCAGGAG 
TATGAACTCG ACGTGATTGC TTCGGATGCA GCCCATGAAG CTCGCACTGT TCTAACTGTG 
CGAGTGAACG ATGAAAACGA CAATGCACCA GTATTCCTGG CCCAACAGCC TCCGGCTTAC 
TTTGCCATTT TGCCAGCTAT ATCCGAGATC AGTGAGAGTC TTTCCGTGGA CTTCGATCTC 
CTAACTGTCA ACGCCACTGA CGCAGACTCG GAGGGTAATA ACTCAAAGGT TATTTATATC 
ATTGAACCTG CCCAGGAGGG CTTCAGTGTG CACCCATCAA ATGGTGTTGT GTCTGTTAAT 
ATGTCTAGAT TACAGCCGGC CGTATCCTCT AGCGGGGATT ATTTTGTAAG AATTATAGCC 
AAGGACGCAG GAAAACCAGC TTTGAAAAGC TCAACACTTT TGAGAGTACA GGCAAATGAC 
AACGGTTCCG GACGTTCGCA ATTCCTACAG AACCAATATA GGGCTCAGAT AAGCGAGGCA 
GCTCCTTTGG GATCGGTTGT TCTCCAGCTT GGACAAGATG CTCTGGATCA ATCCTTAGCT 
ATCATTGCTG GAAATGAAGA ATCCGCTTTT GAGTTGCTCC AATCCAAAGC AATTGTCTTG 
GTCAAGCCCT TGGATAGAGA ACGCAATGAT CTCTACAAAC TGCGATTGGT TTTAAGCCAT 
CCACATGGAC CTCCACTTAT TTCAAGCTTA AATTCCTCGT CTGGAATCTC AGTAATCATA 
ACAATCCTGG ATGCCAACGA TAACTTCCCA ATTTTCGATC GAAGCGCTAA ATATGAGGCT 
GAGATCAGCG AACTTGCTCC TCTGCGGTAC TCCATAGCGC AGTTGCAGGC CATTGATGCC 
GATCAGGAAA ACACCCCAAA CTCAGAAGTG GTATACGACA TAACCTCTGG CAATGATGAG 
CATATGTTTA CGATAGACTT GGTCACCGGC GTTCTCTTTG TGAACAATCG CTTGGACTAC 
GATTCGGGGG CCAAGAGTTA CGAGCTAATA ATCCGAGCCT GCGATAGTCA TCACCAAAGA 
CCACTTTGCA GCTTGCAACC CTTTCGATTG GAACTCCATG ACGAGAACGA CAACGAACCC 
AAGTTCCCGC TAACTGAATA TGTTCACTTC CTGGCTGAAA ACGAGCCGGT TGGCAGCTCC 
GTATTCCGTG CTCATGCCAG TGATTTGGAT AAGGGACCCT TCGGGCAATT AAACTACAGC 
ATAGGTCCTG CTCCCAGCGA CGAGAGCTCT TGGAAAATGT TCCGAGTTGA TTCGGAAAGT 
GGTCTCGTTA CATCCGCCTT CGTATTCGAC TATGAGCAAA GACAGCGCTA TGACATGGAG 
CTGCTCGCCT CCGATATGGG CGGTAAGAAG GCCAGTGTGG CTGTGCGCGT AGAGATTGAG 
TCGAGGGATG AGTTCACCCC GCAGTTCACA GAGCGCACCT ATAGGTTTGT GCTGCCAGCC 
GCCGTGGCCC TACCCCAAGG CTACGTGGTG GGTCAAGTGA CTGCCACTGA TAGTGACAGT 
GGCCCGGACG GACGAGTGGT CTATCAACTA AGTGCCCCAC ACTCGCATTT CAAGGTAAAC 
CGTAGCAGCG GGGCTGTTCT AATCAAGAGG AAACTCAAAC TGGATGGCGA CGGAGACGGT 
AATCTGTACA TGGATGGTCG AGATATCAGT CTAGTTATCT CAGCTTCCTC GGGGCGTCAC 
AACTCCTTAT CCAGCATGGC TGTTGTCGAG ATTGCCTTGG ATCCTTTGGC ACATCCAGGC 
ACAAACCTGG CCAGTGCAGG TGGTTCGTCA AGTGGTTCCA TTGGCGATTG GGCAATTGGT 
CTACTGGTAG CTTTTCTTCT AGTCCTCTGC GCTGCCGCCG GAATCTTTCT ATTCATCCAT 
ATGAGAAGCA GAAAACCGCG TAATGCGGTC AAGCCTCATC TTGCCACGGA CAATGCTGGT 
GTGGGCAATA CCAACAGCTA TGTGGACCCC AGTGCTTTCG ACACGATCCC TATAAGAGGA 
AGCATATCGG GCGGTGCTGC AGGAGCGGCA TCTGGTCAAT TCGCTCCTCC GAAGTACGAC 
GAGATTCCTC CGTTTGGAGC TCATGCTGGC AGCTCCGGAG CAGCTACAAC CTCCGAGTTG 
TCGGGCTCCG AACAGTCCGG TTCCAGTGGC CGAGGCTCTG CTGAGGACGA TGGGGAGGAT 
GAGGAGATTC GTATGATCAA CGAGGGACCA CTACATCACC GCAATGGTGG AGCTGGGGCA 
GGCAGCGATG ATGGCCGAAT TTCGGATATA TCTGTGCAAA ACACCCAGGA ATACTTGGCT 
CGCCTGGGCA TCGTAGATCA CGATCCTAGT GGCGCCGGAG GAGGAGCTTC CAGTATGGCT 
GGTTCAAGTC ACCCAATGCA CTTGTATCAC GATGATGATG CCACAGCACG GTCGGATATT 
ACGAACCTCA TCTATGCCAA ACTTAATGAT GTGACAGGAG CTGGTTCAGA GATAGGAAGC 
AGTGCAGACG ACGCGGGAAC CACTGCGGGC TCCATCGGAA CCATCGGAAC GGCTATAACC 
CATGGCCATG GGGTGATGAG CAGTTACGGA GAAGTTCCTG TTCCAGTACC GGTTGTGGTT 
GGCGGTAGTA ATGTGGGTGG TTCCCTGAGC TCCATAGTTC ACAGTGAGGA GGAATTGACT 
GGCAGCTACA ACTGGGATTA CCTATTGGAT TGGGGACCGC AATATCAGCC ACTTGCGCAT 
GTGTTTTCCG AAATAGCGAG ACTGAAAGAT GACACCTTAT CGGAGCACAG TGGAAGTGGG 
GCTTCCTCGT CCGCCAAGAG CAAGCACTCT TCCTCACATT CCTCAGCAGG AGCAGGAAGC 
GTGGTGCTCA AACCACCACC CTCAGCACCA CCGACTCACA TACCACCCCC CCTGCTAACC 
AATGTGGCAC CAAGGGCCAT TAACCTGCCT ATGCGCTTGC CACCCCATTT GAGCCTGGCC 
CCCGCCCATT TGCCCAGATC GCCCATTGGT CATGAGGCGA GTGGCAGTTT CAGCACATCA 
TCAGCAATGT CACCTTCGTT CTCGCCCTCT CTATCGCCGT TGGCAACAAG ATCACCATCC 
ATTTCTCCTC TGGGAGCGGG ACCACCCACG CACCTGCCTC ATGTCTCGTT GCCACGCCAC 
GGTCATGCCC CTCAGCCAAG TCAGAGGGGA AATGTGGGGA CACGGATGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ds cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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