Pph13 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pph13
Entrez Gene ID	33239
Full Name	PvuII-PstI homology 13
Synonyms	60Mun1,CG2819,Dmel\CG2819,Mu,PPH13,pph13
General protein information	Preferred Names PvuII-PstI homology 13 Names CG2819 gene product from transcript CG2819-RA CG2819-PA Pph13-PA hazy munster
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 21E2-21E2

mRNA and Protein(s)

mRNA	Protein	Name
NM_057982.4	NP_477330.1	PvuII-PstI homology 13

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Authors conclude that Glass can either directly or indirectly initiate the expression of most phototransduction proteins in a Hazy-independent manner, and that Hazy is mainly required for the maintenance of functional photoreceptors in adult flies.
Title: Successive requirement of Glass and Hazy for photoreceptor specification and maintenance in Drosophila.

Although Otd and Pph13 have been implicated in rhabdomere morphogenesis, we demonstrate that it is necessary to remove both factors to completely eliminate rhabdomere formation.
Title: Pph13 and orthodenticle define a dual regulatory pathway for photoreceptor cell morphogenesis and function.

Data show that Pph13 is required for both rhabdomere morphogenesis and for the proper detection of light.
Title: Mutation of the photoreceptor specific homeodomain gene Pph13 results in defects in phototransduction and rhabdomere morphogenesis.

The following Pph13 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pph13 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa13373	NM_057982.4 Latest version!	Drosophila melanogaster PvuII-PstI homology 13 (Pph13), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pph13 ( NM_057982.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477330.1 Drosophila melanogaster PvuII-PstI homology 13 (Pph13), mRNA.

Price & Availability↑

CloneID	OFa13373
Clone ID Related Accession (Same CDS sequence)	NM_057982.4
Accession Version	NM_057982.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	PvuII-PstI homology 13
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_057982.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACAAAG GCACAATGAA GCGCAAGCAG CGGAGATACA GAACCACATT TAACACACTG 
CAACTGCAGG AGCTGGAAAG GGCGTTCCAG CGGACACACT ATCCGGACGT GTTTTTCCGA 
GAGGAGCTGG CCGTGAGGAT CGATTTGACG GAGGCGAGGG TGCAGGTGTG GTTCCAGAAC 
CGGCGGGCCA AGTGGCGCAA GCAGGAGAAG ATCGGTGGGC TTGGCGGAGA TTATAAGGAG 
GGTGCGTTGG ATCTCGACGT GTCCTACGAC GACAGCGCCG TCCTGGGCCA ACTGGATAGT 
GCGCTGGGCG GCGGTGGAAC TCTATTGCCG GACACCCCAC CGCAATCCTC GAACTCCCTT 
GATAACGAAC TTAAGGCCTC CTACGGCACG GGGGCCATGT CGCCCTCTAG GCTGTCACCG 
AATATATTTC TCAACCTCAA CATTGACCAC TTGGGACTGG AGCGGGGTGG CAGTGGCCTC 
TCCATGGAGT GGTCCACATA TCCTCCGCAA ACGCAAGCGC AGACACACCC GCAGATGGAC 
TCCGACAATC AGCTCCAGCA GCACCCGCCT CAACAGCATG CATCCGATCC GATTCACGCC 
GGGAGTTCGA GTCATCACCA GCAGCAGCAG CAGCAGCACC AGCAGGAGCA GCACAATCCC 
CAGCTTCATC CGGGGCTTGA GTTTGCCGCC TCATTGAGTT TGGACATGAC CGATGGCTCC 
TCCGCCTACG ACGAGATGAA GTTTCTCAGC GTGGACGTGG ACCAGTTCAC GATCGACAGC 
TTCAAGGCGG ACTGCATTCT GAGCATGGAG CAGTCCCAGA TGCAGGCCTA CGGCGGTCAC 
TCCCAGCTGG TCGGATCATC CAATGAACTG TGCCTGGACG GGATTGGCAT GTCCTCCTTT 
GGCATGGAAG AGGGTGAGCC CAAGTCGCCT CCCTCTCTTT TGGTGCTGGA CAAGTCGCTG 
CCCTCTTTGA GCATAGGCGT GGAAGGAATC GCTGATCTCG TGGAGCAACT GCATCATCAC 
CAGCATGAGG GCGGCCCAGT GGGCGGTGGT GTCATCACTG GCGATGTCAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477330.1
CDS	508..1581
Translation	MDKGTMKRKQRRYRTTFNTLQLQELERAFQRTHYPDVFFREELAVRIDLTEARVQVWFQNRRAKWRKQEKIGGLGGDYKEGALDLDVSYDDSAVLGQLDSALGGGGTLLPDTPPQSSNSLDNELKASYGTGAMSPSRLSPNIFLNLNIDHLGLERGGSGLSMEWSTYPPQTQAQTHPQMDSDNQLQQHPPQQHASDPIHAGSSSHHQQQQQQHQQEQHNPQLHPGLEFAASLSLDMTDGSSAYDEMKFLSVDVDQFTIDSFKADCILSMEQSQMQAYGGHSQLVGSSNELCLDGIGMSSFGMEEGEPKSPPSLLVLDKSLPSLSIGVEGIADLVEQLHHHQHEGGPVGGGVITGDVM

Target ORF information:

RefSeq Version	NM_057982.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster PvuII-PstI homology 13 (Pph13), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057982.4

ORF Insert Sequence:

ATGGACAAAG GCACAATGAA GCGCAAGCAG CGGAGATACA GAACCACATT TAACACACTG 
CAACTGCAGG AGCTGGAAAG GGCGTTCCAG CGGACACACT ATCCGGACGT GTTTTTCCGA 
GAGGAGCTGG CCGTGAGGAT CGATTTGACG GAGGCGAGGG TGCAGGTGTG GTTCCAGAAC 
CGGCGGGCCA AGTGGCGCAA GCAGGAGAAG ATCGGTGGGC TTGGCGGAGA TTATAAGGAG 
GGTGCGTTGG ATCTCGACGT GTCCTACGAC GACAGCGCCG TCCTGGGCCA ACTGGATAGT 
GCGCTGGGCG GCGGTGGAAC TCTATTGCCG GACACCCCAC CGCAATCCTC GAACTCCCTT 
GATAACGAAC TTAAGGCCTC CTACGGCACG GGGGCCATGT CGCCCTCTAG GCTGTCACCG 
AATATATTTC TCAACCTCAA CATTGACCAC TTGGGACTGG AGCGGGGTGG CAGTGGCCTC 
TCCATGGAGT GGTCCACATA TCCTCCGCAA ACGCAAGCGC AGACACACCC GCAGATGGAC 
TCCGACAATC AGCTCCAGCA GCACCCGCCT CAACAGCATG CATCCGATCC GATTCACGCC 
GGGAGTTCGA GTCATCACCA GCAGCAGCAG CAGCAGCACC AGCAGGAGCA GCACAATCCC 
CAGCTTCATC CGGGGCTTGA GTTTGCCGCC TCATTGAGTT TGGACATGAC CGATGGCTCC 
TCCGCCTACG ACGAGATGAA GTTTCTCAGC GTGGACGTGG ACCAGTTCAC GATCGACAGC 
TTCAAGGCGG ACTGCATTCT GAGCATGGAG CAGTCCCAGA TGCAGGCCTA CGGCGGTCAC 
TCCCAGCTGG TCGGATCATC CAATGAACTG TGCCTGGACG GGATTGGCAT GTCCTCCTTT 
GGCATGGAAG AGGGTGAGCC CAAGTCGCCT CCCTCTCTTT TGGTGCTGGA CAAGTCGCTG 
CCCTCTTTGA GCATAGGCGT GGAAGGAATC GCTGATCTCG TGGAGCAACT GCATCATCAC 
CAGCATGAGG GCGGCCCAGT GGGCGGTGGT GTCATCACTG GCGATGTCAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Pph13 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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