Plc21C cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Plc21C
Entrez Gene ID	33204
Full Name	Phospholipase C at 21C
Synonyms	CG4574,Dmel\CG4574,PLC,PLC-21,PLC-21C,PLC21C,PLC21c,PLC[[Beta]],Plc21,Plcbeta21C,plc-21,plc21,plc21C,plc21c,plcbeta21c
General protein information	Preferred Names Phospholipase C at 21C Names CG4574 gene product from transcript CG4574-RC CG4574-PA CG4574-PB CG4574-PC CG4574-PD CG4574-PG CG4574-PH Plc21C-PA Plc21C-PB Plc21C-PC Plc21C-PD Plc21C-PG Plc21C-PH phospholipase C phospholipase C 21C phospholipase C at 21C
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 21B8-21C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_205884.3	NP_995606.1	phospholipase C at 21C, isoform C
NM_205883.2	NP_995605.1	phospholipase C at 21C, isoform D
NM_057504.5	NP_476852.1	phospholipase C at 21C, isoform A
NM_057503.5	NP_476851.2	phospholipase C at 21C, isoform B
NM_001272889.1	NP_001259818.1	phospholipase C at 21C, isoform G
NM_205882.2	NP_995604.2	phospholipase C at 21C, isoform H

Pathways from BioSystems

Homologies

	PLCB1	XP_001116017.1
Mus musculus (house mouse)	Plcb1	NP_001139302.1
Canis lupus familiaris (dog)	PLCB1	XP_005634858.1
Bos taurus (cattle)	PLCB1	NP_777242.1
Gallus gallus (chicken)	PLCB1	XP_425261.3
Drosophila melanogaster (fruit fly)	Plc21C	NP_995604.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009115	XP_319865.4
Homo sapiens (human)	PLCB1	NP_056007.1
Pan troglodytes (chimpanzee)	PLCB1	XP_001170216.3
Rattus norvegicus (Norway rat)	Plcb1	NP_001071109.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Galphaq, Ggamma1 and Plc21C are anti-obesity genes and Akh employs the Galphaq/Ggamma1/Plc21C module of iCa(2+) control to regulate lipid mobilization in adult Drosophila.
Title: G?q, G?1 and Plc21C control Drosophila body fat storage.

Disruption of PLCb4 function completely blocked light-dependent modulation of Shab currents in Drosophila photoreceptors.
Title: Light-dependent modulation of Shab channels via phosphoinositide depletion in Drosophila photoreceptors.

Reduced odor responses from antennal neurons of phospholipase Cbeta mutants in Drosophila support a role for a phospholipid intermediate in insect olfactory transduction.
Title: Reduced odor responses from antennal neurons of G(q)alpha, phospholipase Cbeta, and rdgA mutants in Drosophila support a role for a phospholipid intermediate in insect olfactory transduction.

These results are consistent with a model in which phosphatidylinositol 4,5-bisphosphate hydrolysis acts via Ca2+ to activate myosin via MLCK and thereby control actin dynamics during constriction of the contractile ring.
Title: Phospholipase C and myosin light chain kinase inhibition define a common step in actin regulation during cytokinesis.

The following Plc21C gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Plc21C cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa13331	NM_205884.3 Latest version!	Drosophila melanogaster phospholipase C at 21C, transcript variant C (Plc21C), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa13332	NM_057504.5 Latest version!	Drosophila melanogaster phospholipase C at 21C, transcript variant A (Plc21C), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa13332	NM_057503.5 Latest version!	Drosophila melanogaster phospholipase C at 21C, transcript variant B (Plc21C), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa13333	NM_205883.2 Latest version!	Drosophila melanogaster phospholipase C at 21C, transcript variant D (Plc21C), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa13334	NM_205882.2 Latest version!	Drosophila melanogaster phospholipase C at 21C, transcript variant H (Plc21C), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa15865	NM_001272889.1 Latest version!	Drosophila melanogaster phospholipase C at 21C, transcript variant G (Plc21C), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Plc21C ( NM_205884.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995606.1 Drosophila melanogaster phospholipase C at 21C, transcript variant C (Plc21C), mRNA.

Price & Availability↑

CloneID	OFa13331
Clone ID Related Accession (Same CDS sequence)	NM_205884.3
Accession Version	NM_205884.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phospholipase C at 21C, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_205884.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGAGCG CCGGAGGAAC CTACATCTCC ACCGCCAGCG TGGAGGTGCC ACAAGCTCTG 
CAAGATGGCG AGAAGTTCAT TCGATGGGAT GATGACTCGG GCACTGGAAC ACCGGTTACA 
ATGCGCGTCG ATGCCAAGGG CTTCTTCCTC TACTGGGTCG ACCAGAACAA CGAGCTGGAT 
ATCCTTGATA TAGCCACCAT ACGCGACGTT CGCACAGGAC AGTACGCTAA GCGACCAAAG 
GACAACAAGC TGCGCCAGAT TGTGACGCTG GGCCCCCAGG ACACGCTCGA GGAGAAGACA 
GTGACGGTAT GCCACGGCTC GGACTTCGTC AACATGACGT TCGTCAACTT CTGCTGCACC 
CGGCGGGACA TAGCCCAGCT CTGGACCGAC GGACTCATCA AGTTGGCCTA CAGCTTGGCC 
CAGCTCAATG GCTCGGCGAT TATGTTCCTG CAGAAGGCCC ATACCAAACT GTGTCTCCAG 
GTGGACAAAA GCGGCCGCAT ACCAGTGAAA AACATCATAA AACTTTTTGC CCAAAACAAG 
GAGGATCGCA AACGTGTCGA GAAGGCGCTG GACGTGACCG GTTTGCCGTC GGGAAAGGTC 
GATAGCATAT CGGTGTCCAA ATTTCAATTC GAGGACTTTT ACAATTTGTA CAAATATCTC 
ACGCAGCGTT CGGAGGTGGA ACGCCTCTTT GACAGCATCG TTGGCAACTC GAAGCGCAAG 
TGCATGTCCA TCGCTCAACT GGTGGAGTTC CTGAACAAGA CACAGCGTGA TCCCCGCCTG 
AACGAGATCC TCTACCCTTA TGCGAATCCC GCCCGCGCCA AGGAGCTCAT CCAGCAATAT 
GAGCCCAACA AGTTCAATGC GCAAAAGGGC CAACTCAGCC TGGATGGCTT TTTGAGGTAC 
TTAATGGGCG ACGACAATCC CATCATGGCG CCCAGCAAGC TCGATCTCTG CGATGACATG 
GACCAGCCGA TGTCGCACTA CTTCATCAAC TCCTCGCATA ACACCTACTT GACGGGACAC 
CAGCTGACCG GCAAGTCGTC CGTCGAGATT TACAGGCAGT GTCTCCTGGC GGGATGCAGA 
TGCGTTGAGC TCGACTTCTG GAACGGACGC ACCGAGGAGC CAGTCATTGT CCACGGCTAT 
ACGTTCGTGC CCGAGATATT TGCCAAGGAC GTGCTGGAGG CCATCGCAGA GAGTGCATTT 
AAAACATCCG AATACCCTGT GATATTGAGC TTTGAGAATC ACTGCAACCC TAGGCAACAG 
GCGAAAATTG CCAACTATTG TCGCGAAATA TTTGGCGATA TGCTGCTCGA TAAGCCTCTG 
GACTCTCATC CGCTGGAGCC GAACATGGAC TTGCCACCGC CGGCGATGCT GCGCCGCAAG 
ATTATAATCA AGAACAAGAA GAAGCATCAC CACCACCATC ACCATCATCA CCACAAAAAG 
CCAGCGCAGG TGGGCACGCC GGCAGCCAAC AACAAACTGA CTACAGCGAA TTCAGTGGAT 
GCCAAGGCGG CGCAACAAGT CGGTTTGTCG GCCTCGCATG AGGATGGGGG CGTGACCAGG 
AGCACCGCCA ACGGTGACGT TGCCACTGGA ACAGGAACCG GAAGTGCTGC TGGGACAGCA 
GGACACGCGC CGCCGTTGCA ACAAATCCGC CAAAGCTCCA AAGACAGCAC TGGATCCTCC 
GATTCGGACA GCTCTTCGGA GGATGAATCC CTGCCTAACA CCACGCCCAA TTTGCCCAGC 
GGCAACGAAC CTCCGCCAGA AAAGGCTCAA AAGGAAACGG AGGCGGGAGC AGAGATTTCA 
GCTTTGGTCA ACTATGTTCA GCCCATCCAT TTTAGCTCTT TTGAGAATGC GGAAAAAAAG 
AATCGATGCT ACGAAATGTC TTCGTTTGAT GAAAAACAGG CGACAACATT ATTGAAGGAG 
CGACCGATTG AATTTGTGAA CTACAACAAA CACCAATTAT CTCGTGTCTA TCCGGCGGGC 
ACTCGCTTTG ATAGCTCCAA CTTTATGCCG CAGTTGTTCT GGAATGCCGG ATGTCAGCTC 
GTGGCACTTA ACTTCCAAAC CCTCGATCTG GCCATGCAAC TCAACTTGGG GATCTTTGAG 
TATAACGCTC GGTCCGGTTA TCTACTTAAG CCGGAGTTTA TGCGCCGATC AGATCGCAGA 
TTGGATCCCT TCGCGGAAAG CACTGTGGAT GGCATCATAG CAGGCACAGT ATCAATCACG 
GTTCTATCTG GTCAGTTTCT CACGGATAAG AGGGCGAACA CTTTTGTGGA AGTTGATATG 
TACGGTTTGC CAGCGGACAC GGTGAGAAAG AAGTTTCGCA CCAAGACAGT GCGGGATAAT 
GGAATGAATC CCCTTTATGA CGAGGAGCCG TTCGTGTTTA AGAAGGTGGT TCTCCCCGAG 
TTGGCGAGCA TTCGAATTGC AGCATATGAA GAGGGTGGCA AACTCATTGG TCACCGCGTT 
TTGCCCGTAA TCGGTCTTTG CCCTGGTTAC CGACATGTGA ATCTCCGATC TGAAGTGGGT 
CAACCAATAG CCCTTGCCTC TCTTTTCCTG TGCGTGGTGG TCAAGGATTA TGTACCCGAT 
GATCTTTCCA ATTTCGCAGA GGCGCTAGCT AACCCTATTA AATACCAAAG TGAGCTAGAG 
AAGCGCGATA TTCAACTATC TGTTTTGACC GACGAAGCCG AAGCGCTGGG CAGTGCGGAC 
GAGGATCTCT CCAAGTCGTT CGTTTTCGTC CAAGTAGGTG GCCAAAAAAA GGAGCTGCGT 
CCGGTTGAAT CGCTTGCGAC TTCGCCGAAG CATAGGCCGA GCATCTCGGC CGCCGCAGCA 
ATGAGTGTTG ACGTTACCGT GGATCGGACC GATGGCGGAA GGGGAGAAGA TAGCATCTCT 
ATTGTAGCAC CCTCAATACA GCATCAGCAC TCTCTAGACC AGTCCGTAAG CACCTCCATC 
CGACAGGTGG AGTCCTCGCA GTTTGATGTC GATCTTGTGT TGGCGGAGCC GCTGGAAAAG 
ATATTGGATC ACAAGTCCGT CAAGGAAAAG CGTCTCGAGA TGGAAAAGAA GCTGGAGTCA 
CTGCGAAAGA AGCATGACAA GGAAAAGATT AAGATCGCCG GCCAGAAATC CAGTCCGCTA 
GAGGGCAAGA AGCCGAAGTT TGCCATCACG AATAAGCTGG TGAAGCGGCT AAGCAACAAG 
AGTCTCGAGC CTGGTGTAGA GATCCCCGCC TGCCCTCTGG ATCTGGGCGA TAGCAGCGAG 
GAGAGTGCAG CCGCGGATGC CGGTGAGGAC TTAGCCGGTG GGAGCAGCAG TCTGGATGGC 
AGAACTCAAG AGTCGCGATT ACGCAGTGCC TGCCGGGAGT ACACTTCCCA GTACCGTGAG 
CTGCAGGAGA AGTACCATGA GGCAATTTAC AGTGCAGCCG AGAAGGTGCT GAAGACATCG 
CAAACTGGGC AAACAAAGCA GTTAAAAGCA TCTCTGGACA AGGTCACTGG AGAGGTTATG 
CACCAGCTGC AGGAGGCCCG CCGCAACGAG GTTAAGAATC TGGCCACCGT GCACCGGGAT 
CGTGATGAGC TGATCAGAAT GAAACGCGAG GTGGCAAGCT CCGTTGTGGA ACGTGGCGTA 
GCTGAACGTG TGCGGCTGAA GCAGACCTTT GACCGGAGAA CGGATGAACT TCAGAAGCAG 
CACGACTCAG TGCGCAATGC CCTGGCAGAG CACCGCTCCA AGGCTCGTCA AATTCTCGAC 
AAGGAGGCAG AATCGCGAAG CTGTGTATCC AGCAATGGCT TCTTGGTCCT CTTCCATGGA 
CCTCATCACC ATGGCTGTAC GGGCTCCGGC TCGTCAGCCC TTTCAGGCAA TAATCTCACT 
CTAAATCTGG ACGCGGGGGC TGCCGGTAGT CATTCAGCCA TTTCGCCGGC AAAGTCGCAC 
AACAGCATCG CAGCAGCGGC CGAGATGAAG ACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995606.1
CDS	741..4676
Translation	MMSAGGTYISTASVEVPQALQDGEKFIRWDDDSGTGTPVTMRVDAKGFFLYWVDQNNELDILDIATIRDVRTGQYAKRPKDNKLRQIVTLGPQDTLEEKTVTVCHGSDFVNMTFVNFCCTRRDIAQLWTDGLIKLAYSLAQLNGSAIMFLQKAHTKLCLQVDKSGRIPVKNIIKLFAQNKEDRKRVEKALDVTGLPSGKVDSISVSKFQFEDFYNLYKYLTQRSEVERLFDSIVGNSKRKCMSIAQLVEFLNKTQRDPRLNEILYPYANPARAKELIQQYEPNKFNAQKGQLSLDGFLRYLMGDDNPIMAPSKLDLCDDMDQPMSHYFINSSHNTYLTGHQLTGKSSVEIYRQCLLAGCRCVELDFWNGRTEEPVIVHGYTFVPEIFAKDVLEAIAESAFKTSEYPVILSFENHCNPRQQAKIANYCREIFGDMLLDKPLDSHPLEPNMDLPPPAMLRRKIIIKNKKKHHHHHHHHHHKKPAQVGTPAANNKLTTANSVDAKAAQQVGLSASHEDGGVTRSTANGDVATGTGTGSAAGTAGHAPPLQQIRQSSKDSTGSSDSDSSSEDESLPNTTPNLPSGNEPPPEKAQKETEAGAEISALVNYVQPIHFSSFENAEKKNRCYEMSSFDEKQATTLLKERPIEFVNYNKHQLSRVYPAGTRFDSSNFMPQLFWNAGCQLVALNFQTLDLAMQLNLGIFEYNARSGYLLKPEFMRRSDRRLDPFAESTVDGIIAGTVSITVLSGQFLTDKRANTFVEVDMYGLPADTVRKKFRTKTVRDNGMNPLYDEEPFVFKKVVLPELASIRIAAYEEGGKLIGHRVLPVIGLCPGYRHVNLRSEVGQPIALASLFLCVVVKDYVPDDLSNFAEALANPIKYQSELEKRDIQLSVLTDEAEALGSADEDLSKSFVFVQVGGQKKELRPVESLATSPKHRPSISAAAAMSVDVTVDRTDGGRGEDSISIVAPSIQHQHSLDQSVSTSIRQVESSQFDVDLVLAEPLEKILDHKSVKEKRLEMEKKLESLRKKHDKEKIKIAGQKSSPLEGKKPKFAITNKLVKRLSNKSLEPGVEIPACPLDLGDSSEESAAADAGEDLAGGSSSLDGRTQESRLRSACREYTSQYRELQEKYHEAIYSAAEKVLKTSQTGQTKQLKASLDKVTGEVMHQLQEARRNEVKNLATVHRDRDELIRMKREVASSVVERGVAERVRLKQTFDRRTDELQKQHDSVRNALAEHRSKARQILDKEAESRSCVSSNGFLVLFHGPHHHGCTGSGSSALSGNNLTLNLDAGAAGSHSAISPAKSHNSIAAAAEMKT

Target ORF information:

RefSeq Version	NM_205884.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phospholipase C at 21C, transcript variant C (Plc21C), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205884.3

ORF Insert Sequence:

ATGATGAGCG CCGGAGGAAC CTACATCTCC ACCGCCAGCG TGGAGGTGCC ACAAGCTCTG 
CAAGATGGCG AGAAGTTCAT TCGATGGGAT GATGACTCGG GCACTGGAAC ACCGGTTACA 
ATGCGCGTCG ATGCCAAGGG CTTCTTCCTC TACTGGGTCG ACCAGAACAA CGAGCTGGAT 
ATCCTTGATA TAGCCACCAT ACGCGACGTT CGCACAGGAC AGTACGCTAA GCGACCAAAG 
GACAACAAGC TGCGCCAGAT TGTGACGCTG GGCCCCCAGG ACACGCTCGA GGAGAAGACA 
GTGACGGTAT GCCACGGCTC GGACTTCGTC AACATGACGT TCGTCAACTT CTGCTGCACC 
CGGCGGGACA TAGCCCAGCT CTGGACCGAC GGACTCATCA AGTTGGCCTA CAGCTTGGCC 
CAGCTCAATG GCTCGGCGAT TATGTTCCTG CAGAAGGCCC ATACCAAACT GTGTCTCCAG 
GTGGACAAAA GCGGCCGCAT ACCAGTGAAA AACATCATAA AACTTTTTGC CCAAAACAAG 
GAGGATCGCA AACGTGTCGA GAAGGCGCTG GACGTGACCG GTTTGCCGTC GGGAAAGGTC 
GATAGCATAT CGGTGTCCAA ATTTCAATTC GAGGACTTTT ACAATTTGTA CAAATATCTC 
ACGCAGCGTT CGGAGGTGGA ACGCCTCTTT GACAGCATCG TTGGCAACTC GAAGCGCAAG 
TGCATGTCCA TCGCTCAACT GGTGGAGTTC CTGAACAAGA CACAGCGTGA TCCCCGCCTG 
AACGAGATCC TCTACCCTTA TGCGAATCCC GCCCGCGCCA AGGAGCTCAT CCAGCAATAT 
GAGCCCAACA AGTTCAATGC GCAAAAGGGC CAACTCAGCC TGGATGGCTT TTTGAGGTAC 
TTAATGGGCG ACGACAATCC CATCATGGCG CCCAGCAAGC TCGATCTCTG CGATGACATG 
GACCAGCCGA TGTCGCACTA CTTCATCAAC TCCTCGCATA ACACCTACTT GACGGGACAC 
CAGCTGACCG GCAAGTCGTC CGTCGAGATT TACAGGCAGT GTCTCCTGGC GGGATGCAGA 
TGCGTTGAGC TCGACTTCTG GAACGGACGC ACCGAGGAGC CAGTCATTGT CCACGGCTAT 
ACGTTCGTGC CCGAGATATT TGCCAAGGAC GTGCTGGAGG CCATCGCAGA GAGTGCATTT 
AAAACATCCG AATACCCTGT GATATTGAGC TTTGAGAATC ACTGCAACCC TAGGCAACAG 
GCGAAAATTG CCAACTATTG TCGCGAAATA TTTGGCGATA TGCTGCTCGA TAAGCCTCTG 
GACTCTCATC CGCTGGAGCC GAACATGGAC TTGCCACCGC CGGCGATGCT GCGCCGCAAG 
ATTATAATCA AGAACAAGAA GAAGCATCAC CACCACCATC ACCATCATCA CCACAAAAAG 
CCAGCGCAGG TGGGCACGCC GGCAGCCAAC AACAAACTGA CTACAGCGAA TTCAGTGGAT 
GCCAAGGCGG CGCAACAAGT CGGTTTGTCG GCCTCGCATG AGGATGGGGG CGTGACCAGG 
AGCACCGCCA ACGGTGACGT TGCCACTGGA ACAGGAACCG GAAGTGCTGC TGGGACAGCA 
GGACACGCGC CGCCGTTGCA ACAAATCCGC CAAAGCTCCA AAGACAGCAC TGGATCCTCC 
GATTCGGACA GCTCTTCGGA GGATGAATCC CTGCCTAACA CCACGCCCAA TTTGCCCAGC 
GGCAACGAAC CTCCGCCAGA AAAGGCTCAA AAGGAAACGG AGGCGGGAGC AGAGATTTCA 
GCTTTGGTCA ACTATGTTCA GCCCATCCAT TTTAGCTCTT TTGAGAATGC GGAAAAAAAG 
AATCGATGCT ACGAAATGTC TTCGTTTGAT GAAAAACAGG CGACAACATT ATTGAAGGAG 
CGACCGATTG AATTTGTGAA CTACAACAAA CACCAATTAT CTCGTGTCTA TCCGGCGGGC 
ACTCGCTTTG ATAGCTCCAA CTTTATGCCG CAGTTGTTCT GGAATGCCGG ATGTCAGCTC 
GTGGCACTTA ACTTCCAAAC CCTCGATCTG GCCATGCAAC TCAACTTGGG GATCTTTGAG 
TATAACGCTC GGTCCGGTTA TCTACTTAAG CCGGAGTTTA TGCGCCGATC AGATCGCAGA 
TTGGATCCCT TCGCGGAAAG CACTGTGGAT GGCATCATAG CAGGCACAGT ATCAATCACG 
GTTCTATCTG GTCAGTTTCT CACGGATAAG AGGGCGAACA CTTTTGTGGA AGTTGATATG 
TACGGTTTGC CAGCGGACAC GGTGAGAAAG AAGTTTCGCA CCAAGACAGT GCGGGATAAT 
GGAATGAATC CCCTTTATGA CGAGGAGCCG TTCGTGTTTA AGAAGGTGGT TCTCCCCGAG 
TTGGCGAGCA TTCGAATTGC AGCATATGAA GAGGGTGGCA AACTCATTGG TCACCGCGTT 
TTGCCCGTAA TCGGTCTTTG CCCTGGTTAC CGACATGTGA ATCTCCGATC TGAAGTGGGT 
CAACCAATAG CCCTTGCCTC TCTTTTCCTG TGCGTGGTGG TCAAGGATTA TGTACCCGAT 
GATCTTTCCA ATTTCGCAGA GGCGCTAGCT AACCCTATTA AATACCAAAG TGAGCTAGAG 
AAGCGCGATA TTCAACTATC TGTTTTGACC GACGAAGCCG AAGCGCTGGG CAGTGCGGAC 
GAGGATCTCT CCAAGTCGTT CGTTTTCGTC CAAGTAGGTG GCCAAAAAAA GGAGCTGCGT 
CCGGTTGAAT CGCTTGCGAC TTCGCCGAAG CATAGGCCGA GCATCTCGGC CGCCGCAGCA 
ATGAGTGTTG ACGTTACCGT GGATCGGACC GATGGCGGAA GGGGAGAAGA TAGCATCTCT 
ATTGTAGCAC CCTCAATACA GCATCAGCAC TCTCTAGACC AGTCCGTAAG CACCTCCATC 
CGACAGGTGG AGTCCTCGCA GTTTGATGTC GATCTTGTGT TGGCGGAGCC GCTGGAAAAG 
ATATTGGATC ACAAGTCCGT CAAGGAAAAG CGTCTCGAGA TGGAAAAGAA GCTGGAGTCA 
CTGCGAAAGA AGCATGACAA GGAAAAGATT AAGATCGCCG GCCAGAAATC CAGTCCGCTA 
GAGGGCAAGA AGCCGAAGTT TGCCATCACG AATAAGCTGG TGAAGCGGCT AAGCAACAAG 
AGTCTCGAGC CTGGTGTAGA GATCCCCGCC TGCCCTCTGG ATCTGGGCGA TAGCAGCGAG 
GAGAGTGCAG CCGCGGATGC CGGTGAGGAC TTAGCCGGTG GGAGCAGCAG TCTGGATGGC 
AGAACTCAAG AGTCGCGATT ACGCAGTGCC TGCCGGGAGT ACACTTCCCA GTACCGTGAG 
CTGCAGGAGA AGTACCATGA GGCAATTTAC AGTGCAGCCG AGAAGGTGCT GAAGACATCG 
CAAACTGGGC AAACAAAGCA GTTAAAAGCA TCTCTGGACA AGGTCACTGG AGAGGTTATG 
CACCAGCTGC AGGAGGCCCG CCGCAACGAG GTTAAGAATC TGGCCACCGT GCACCGGGAT 
CGTGATGAGC TGATCAGAAT GAAACGCGAG GTGGCAAGCT CCGTTGTGGA ACGTGGCGTA 
GCTGAACGTG TGCGGCTGAA GCAGACCTTT GACCGGAGAA CGGATGAACT TCAGAAGCAG 
CACGACTCAG TGCGCAATGC CCTGGCAGAG CACCGCTCCA AGGCTCGTCA AATTCTCGAC 
AAGGAGGCAG AATCGCGAAG CTGTGTATCC AGCAATGGCT TCTTGGTCCT CTTCCATGGA 
CCTCATCACC ATGGCTGTAC GGGCTCCGGC TCGTCAGCCC TTTCAGGCAA TAATCTCACT 
CTAAATCTGG ACGCGGGGGC TGCCGGTAGT CATTCAGCCA TTTCGCCGGC AAAGTCGCAC 
AACAGCATCG CAGCAGCGGC CGAGATGAAG ACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plc21C ( NM_057504.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476852.1 Drosophila melanogaster phospholipase C at 21C, transcript variant A (Plc21C), mRNA.

Price & Availability↑

CloneID	OFa13332
Clone ID Related Accession (Same CDS sequence)	NM_057504.5 , NM_057503.5
Accession Version	NM_057504.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phospholipase C at 21C, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_057504.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGAGCG CCGGAGGAAC CTACATCTCC ACCGCCAGCG TGGAGGTGCC ACAAGCTCTG 
CAAGATGGCG AGAAGTTCAT TCGATGGGAT GATGACTCGG GCACTGGAAC ACCGGTTACA 
ATGCGCGTCG ATGCCAAGGG CTTCTTCCTC TACTGGGTCG ACCAGAACAA CGAGCTGGAT 
ATCCTTGATA TAGCCACCAT ACGCGACGTT CGCACAGGAC AGTACGCTAA GCGACCAAAG 
GACAACAAGC TGCGCCAGAT TGTGACGCTG GGCCCCCAGG ACACGCTCGA GGAGAAGACA 
GTGACGGTAT GCCACGGCTC GGACTTCGTC AACATGACGT TCGTCAACTT CTGCTGCACC 
CGGCGGGACA TAGCCCAGCT CTGGACCGAC GGACTCATCA AGTTGGCCTA CAGCTTGGCC 
CAGCTCAATG GCTCGGCGAT TATGTTCCTG CAGAAGGCCC ATACCAAACT GTGTCTCCAG 
GTGGACAAAA GCGGCCGCAT ACCAGTGAAA AACATCATAA AACTTTTTGC CCAAAACAAG 
GAGGATCGCA AACGTGTCGA GAAGGCGCTG GACGTGACCG GTTTGCCGTC GGGAAAGGTC 
GATAGCATAT CGGTGTCCAA ATTTCAATTC GAGGACTTTT ACAATTTGTA CAAATATCTC 
ACGCAGCGTT CGGAGGTGGA ACGCCTCTTT GACAGCATCG TTGGCAACTC GAAGCGCAAG 
TGCATGTCCA TCGCTCAACT GGTGGAGTTC CTGAACAAGA CACAGCGTGA TCCCCGCCTG 
AACGAGATCC TCTACCCTTA TGCGAATCCC GCCCGCGCCA AGGAGCTCAT CCAGCAATAT 
GAGCCCAACA AGTTCAATGC GCAAAAGGGC CAACTCAGCC TGGATGGCTT TTTGAGGTAC 
TTAATGGGCG ACGACAATCC CATCATGGCG CCCAGCAAGC TCGATCTCTG CGATGACATG 
GACCAGCCGA TGTCGCACTA CTTCATCAAC TCCTCGCATA ACACCTACTT GACGGGACAC 
CAGCTGACCG GCAAGTCGTC CGTCGAGATT TACAGGCAGT GTCTCCTGGC GGGATGCAGA 
TGCGTTGAGC TCGACTTCTG GAACGGACGC ACCGAGGAGC CAGTCATTGT CCACGGCTAT 
ACGTTCGTGC CCGAGATATT TGCCAAGGAC GTGCTGGAGG CCATCGCAGA GAGTGCATTT 
AAAACATCCG AATACCCTGT GATATTGAGC TTTGAGAATC ACTGCAACCC TAGGCAACAG 
GCGAAAATTG CCAACTATTG TCGCGAAATA TTTGGCGATA TGCTGCTCGA TAAGCCTCTG 
GACTCTCATC CGCTGGAGCC GAACATGGAC TTGCCACCGC CGGCGATGCT GCGCCGCAAG 
ATTATAATCA AGAACAAGAA GAAGCATCAC CACCACCATC ACCATCATCA CCACAAAAAG 
CCAGCGCAGG TGGGCACGCC GGCAGCCAAC AACAAACTGA CTACAGCGAA TTCAGTGGAT 
GCCAAGGCGG CGCAACAAGT CGGTTTGTCG GCCTCGCATG AGGATGGGGG CGTGACCAGG 
AGCACCGCCA ACGGTGACGT TGCCACTGGA ACAGGAACCG GAAGTGCTGC TGGGACAGCA 
GGACACGCGC CGCCGTTGCA ACAAATCCGC CAAAGCTCCA AAGACAGCAC TGGATCCTCC 
GATTCGGACA GCTCTTCGGA GGATGAATCC CTGCCTAACA CCACGCCCAA TTTGCCCAGC 
GGCAACGAAC CTCCGCCAGA AAAGGCTCAA AAGGAAACGG AGGCGGGAGC AGAGATTTCA 
GCTTTGGTCA ACTATGTTCA GCCCATCCAT TTTAGCTCTT TTGAGAATGC GGAAAAAAAG 
AATCGATGCT ACGAAATGTC TTCGTTTGAT GAAAAACAGG CGACAACATT ATTGAAGGAG 
CGACCGATTG AATTTGTGAA CTACAACAAA CACCAATTAT CTCGTGTCTA TCCGGCGGGC 
ACTCGCTTTG ATAGCTCCAA CTTTATGCCG CAGTTGTTCT GGAATGCCGG ATGTCAGCTC 
GTGGCACTTA ACTTCCAAAC CCTCGATCTG GCCATGCAAC TCAACTTGGG GATCTTTGAG 
TATAACGCTC GGTCCGGTTA TCTACTTAAG CCGGAGTTTA TGCGCCGATC AGATCGCAGA 
TTGGATCCCT TCGCGGAAAG CACTGTGGAT GGCATCATAG CAGGCACAGT ATCAATCACG 
GTTCTATCTG GTCAGTTTCT CACGGATAAG AGGGCGAACA CTTTTGTGGA AGTTGATATG 
TACGGTTTGC CAGCGGACAC GGTGAGAAAG AAGTTTCGCA CCAAGACAGT GCGGGATAAT 
GGAATGAATC CCCTTTATGA CGAGGAGCCG TTCGTGTTTA AGAAGGTGGT TCTCCCCGAG 
TTGGCGAGCA TTCGAATTGC AGCATATGAA GAGGGTGGCA AACTCATTGG TCACCGCGTT 
TTGCCCGTAA TCGGTCTTTG CCCTGGTTAC CGACATGTGA ATCTCCGATC TGAAGTGGGT 
CAACCAATAG CCCTTGCCTC TCTTTTCCTG TGCGTGGTGG TCAAGGATTA TGTACCCGAT 
GATCTTTCCA ATTTCGCAGA GGCGCTAGCT AACCCTATTA AATACCAAAG TGAGCTAGAG 
AAGCGCGATA TTCAACTATC TGTTTTGACC GACGAAGCCG AAGCGCTGGG CAGTGCGGAC 
GAGGATCTCT CCAAGTCGTG TGGCCAAAAA AAGGAGCTGC GTCCGGTTGA ATCGCTTGCG 
ACTTCGCCGA AGCATAGGCC GAGCATCTCG GCCGCCGCAG CAATGAGTGT TGACGTTACC 
GTGGATCGGA CCGATGGCGG AAGGGGAGAA GATAGCATCT CTATTGTAGC ACCCTCAATA 
CAGCATCAGC ACTCTCTAGA CCAGTCCGTA AGCACCTCCA TCCGACAGGT GGAGTCCTCG 
CAGTTTGATG TCGATCTTGT GTTGGCGGAG CCGCTGGAAA AGATATTGGA TCACAAGTCC 
GTCAAGGAAA AGCGTCTCGA GATGGAAAAG AAGCTGGAGT CACTGCGAAA GAAGCATGAC 
AAGGAAAAGA TTAAGATCGC CGGCCAGAAA TCCAGTCCGC TAGAGGGCAA GAAGCCGAAG 
TTTGCCATCA CGAATAAGCT GGTGAAGCGG CTAAGCAACA AGAGTCTTAA TTGTCTTTCT 
CCGCACAGCG AGCCTGGTGT AGAGATCCCC GCCTGCCCTC TGGATCTGGG CGATAGCAGC 
GAGGAGAGTG CAGCCGCGGA TGCCGGTGAG GACTTAGCCG GTGGGAGCAG CAGTCTGGAT 
GGCAGAACTC AAGAGTCGCG ATTACGCAGT GCCTGCCGGG AGTACACTTC CCAGTACCGT 
GAGCTGCAGG AGAAGTACCA TGAGGCAATT TACAGTGCAG CCGAGAAGGT GCTGAAGACA 
TCGCAAACTG GGCAAACAAA GCAGTTAAAA GCATCTCTGG ACAAGGTCAC TGGAGAGGTT 
ATGCACCAGC TGCAGGAGGC CCGCCGCAAC GAGGTTAAGA ATCTGGCCAC CGTGCACCGG 
GATCGTGATG AGCTGATCAG AATGAAACGC GAGGTGGCAA GCTCCGTTGT GGAACGTGGC 
GTAGCTGAAC GTGTGCGGCT GAAGCAGACC TTTGACCGGA GAACGGATGA ACTTCAGAAG 
CAGCACGACT CAGTGCGCAA TGCCCTGGCA GAGCACCGCT CCAAGGCTCG TCAAATTCTC 
GACAAGGAGG CAGAATCGCG AAGCTGTGTA TCCAGCAATG GCTTCTTGGT CCTCTTCCAT 
GGACCTCATC ACCATGGCTG TACGGGCTCC GGCTCGTCAG CCCTTTCAGG CAATAATCTC 
ACTCTAAATC TGGACGCGGG GGCTGCCGGT AGTCATTCAG CCATTTCGCC GGCAAAGTCG 
CACAACAGCA TCGCAGCAGC GGCCGAGATG AAGACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476852.1
CDS	741..4679
Translation	MMSAGGTYISTASVEVPQALQDGEKFIRWDDDSGTGTPVTMRVDAKGFFLYWVDQNNELDILDIATIRDVRTGQYAKRPKDNKLRQIVTLGPQDTLEEKTVTVCHGSDFVNMTFVNFCCTRRDIAQLWTDGLIKLAYSLAQLNGSAIMFLQKAHTKLCLQVDKSGRIPVKNIIKLFAQNKEDRKRVEKALDVTGLPSGKVDSISVSKFQFEDFYNLYKYLTQRSEVERLFDSIVGNSKRKCMSIAQLVEFLNKTQRDPRLNEILYPYANPARAKELIQQYEPNKFNAQKGQLSLDGFLRYLMGDDNPIMAPSKLDLCDDMDQPMSHYFINSSHNTYLTGHQLTGKSSVEIYRQCLLAGCRCVELDFWNGRTEEPVIVHGYTFVPEIFAKDVLEAIAESAFKTSEYPVILSFENHCNPRQQAKIANYCREIFGDMLLDKPLDSHPLEPNMDLPPPAMLRRKIIIKNKKKHHHHHHHHHHKKPAQVGTPAANNKLTTANSVDAKAAQQVGLSASHEDGGVTRSTANGDVATGTGTGSAAGTAGHAPPLQQIRQSSKDSTGSSDSDSSSEDESLPNTTPNLPSGNEPPPEKAQKETEAGAEISALVNYVQPIHFSSFENAEKKNRCYEMSSFDEKQATTLLKERPIEFVNYNKHQLSRVYPAGTRFDSSNFMPQLFWNAGCQLVALNFQTLDLAMQLNLGIFEYNARSGYLLKPEFMRRSDRRLDPFAESTVDGIIAGTVSITVLSGQFLTDKRANTFVEVDMYGLPADTVRKKFRTKTVRDNGMNPLYDEEPFVFKKVVLPELASIRIAAYEEGGKLIGHRVLPVIGLCPGYRHVNLRSEVGQPIALASLFLCVVVKDYVPDDLSNFAEALANPIKYQSELEKRDIQLSVLTDEAEALGSADEDLSKSCGQKKELRPVESLATSPKHRPSISAAAAMSVDVTVDRTDGGRGEDSISIVAPSIQHQHSLDQSVSTSIRQVESSQFDVDLVLAEPLEKILDHKSVKEKRLEMEKKLESLRKKHDKEKIKIAGQKSSPLEGKKPKFAITNKLVKRLSNKSLNCLSPHSEPGVEIPACPLDLGDSSEESAAADAGEDLAGGSSSLDGRTQESRLRSACREYTSQYRELQEKYHEAIYSAAEKVLKTSQTGQTKQLKASLDKVTGEVMHQLQEARRNEVKNLATVHRDRDELIRMKREVASSVVERGVAERVRLKQTFDRRTDELQKQHDSVRNALAEHRSKARQILDKEAESRSCVSSNGFLVLFHGPHHHGCTGSGSSALSGNNLTLNLDAGAAGSHSAISPAKSHNSIAAAAEMKT

Target ORF information:

RefSeq Version	NM_057504.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phospholipase C at 21C, transcript variant A (Plc21C), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057504.5

ORF Insert Sequence:

ATGATGAGCG CCGGAGGAAC CTACATCTCC ACCGCCAGCG TGGAGGTGCC ACAAGCTCTG 
CAAGATGGCG AGAAGTTCAT TCGATGGGAT GATGACTCGG GCACTGGAAC ACCGGTTACA 
ATGCGCGTCG ATGCCAAGGG CTTCTTCCTC TACTGGGTCG ACCAGAACAA CGAGCTGGAT 
ATCCTTGATA TAGCCACCAT ACGCGACGTT CGCACAGGAC AGTACGCTAA GCGACCAAAG 
GACAACAAGC TGCGCCAGAT TGTGACGCTG GGCCCCCAGG ACACGCTCGA GGAGAAGACA 
GTGACGGTAT GCCACGGCTC GGACTTCGTC AACATGACGT TCGTCAACTT CTGCTGCACC 
CGGCGGGACA TAGCCCAGCT CTGGACCGAC GGACTCATCA AGTTGGCCTA CAGCTTGGCC 
CAGCTCAATG GCTCGGCGAT TATGTTCCTG CAGAAGGCCC ATACCAAACT GTGTCTCCAG 
GTGGACAAAA GCGGCCGCAT ACCAGTGAAA AACATCATAA AACTTTTTGC CCAAAACAAG 
GAGGATCGCA AACGTGTCGA GAAGGCGCTG GACGTGACCG GTTTGCCGTC GGGAAAGGTC 
GATAGCATAT CGGTGTCCAA ATTTCAATTC GAGGACTTTT ACAATTTGTA CAAATATCTC 
ACGCAGCGTT CGGAGGTGGA ACGCCTCTTT GACAGCATCG TTGGCAACTC GAAGCGCAAG 
TGCATGTCCA TCGCTCAACT GGTGGAGTTC CTGAACAAGA CACAGCGTGA TCCCCGCCTG 
AACGAGATCC TCTACCCTTA TGCGAATCCC GCCCGCGCCA AGGAGCTCAT CCAGCAATAT 
GAGCCCAACA AGTTCAATGC GCAAAAGGGC CAACTCAGCC TGGATGGCTT TTTGAGGTAC 
TTAATGGGCG ACGACAATCC CATCATGGCG CCCAGCAAGC TCGATCTCTG CGATGACATG 
GACCAGCCGA TGTCGCACTA CTTCATCAAC TCCTCGCATA ACACCTACTT GACGGGACAC 
CAGCTGACCG GCAAGTCGTC CGTCGAGATT TACAGGCAGT GTCTCCTGGC GGGATGCAGA 
TGCGTTGAGC TCGACTTCTG GAACGGACGC ACCGAGGAGC CAGTCATTGT CCACGGCTAT 
ACGTTCGTGC CCGAGATATT TGCCAAGGAC GTGCTGGAGG CCATCGCAGA GAGTGCATTT 
AAAACATCCG AATACCCTGT GATATTGAGC TTTGAGAATC ACTGCAACCC TAGGCAACAG 
GCGAAAATTG CCAACTATTG TCGCGAAATA TTTGGCGATA TGCTGCTCGA TAAGCCTCTG 
GACTCTCATC CGCTGGAGCC GAACATGGAC TTGCCACCGC CGGCGATGCT GCGCCGCAAG 
ATTATAATCA AGAACAAGAA GAAGCATCAC CACCACCATC ACCATCATCA CCACAAAAAG 
CCAGCGCAGG TGGGCACGCC GGCAGCCAAC AACAAACTGA CTACAGCGAA TTCAGTGGAT 
GCCAAGGCGG CGCAACAAGT CGGTTTGTCG GCCTCGCATG AGGATGGGGG CGTGACCAGG 
AGCACCGCCA ACGGTGACGT TGCCACTGGA ACAGGAACCG GAAGTGCTGC TGGGACAGCA 
GGACACGCGC CGCCGTTGCA ACAAATCCGC CAAAGCTCCA AAGACAGCAC TGGATCCTCC 
GATTCGGACA GCTCTTCGGA GGATGAATCC CTGCCTAACA CCACGCCCAA TTTGCCCAGC 
GGCAACGAAC CTCCGCCAGA AAAGGCTCAA AAGGAAACGG AGGCGGGAGC AGAGATTTCA 
GCTTTGGTCA ACTATGTTCA GCCCATCCAT TTTAGCTCTT TTGAGAATGC GGAAAAAAAG 
AATCGATGCT ACGAAATGTC TTCGTTTGAT GAAAAACAGG CGACAACATT ATTGAAGGAG 
CGACCGATTG AATTTGTGAA CTACAACAAA CACCAATTAT CTCGTGTCTA TCCGGCGGGC 
ACTCGCTTTG ATAGCTCCAA CTTTATGCCG CAGTTGTTCT GGAATGCCGG ATGTCAGCTC 
GTGGCACTTA ACTTCCAAAC CCTCGATCTG GCCATGCAAC TCAACTTGGG GATCTTTGAG 
TATAACGCTC GGTCCGGTTA TCTACTTAAG CCGGAGTTTA TGCGCCGATC AGATCGCAGA 
TTGGATCCCT TCGCGGAAAG CACTGTGGAT GGCATCATAG CAGGCACAGT ATCAATCACG 
GTTCTATCTG GTCAGTTTCT CACGGATAAG AGGGCGAACA CTTTTGTGGA AGTTGATATG 
TACGGTTTGC CAGCGGACAC GGTGAGAAAG AAGTTTCGCA CCAAGACAGT GCGGGATAAT 
GGAATGAATC CCCTTTATGA CGAGGAGCCG TTCGTGTTTA AGAAGGTGGT TCTCCCCGAG 
TTGGCGAGCA TTCGAATTGC AGCATATGAA GAGGGTGGCA AACTCATTGG TCACCGCGTT 
TTGCCCGTAA TCGGTCTTTG CCCTGGTTAC CGACATGTGA ATCTCCGATC TGAAGTGGGT 
CAACCAATAG CCCTTGCCTC TCTTTTCCTG TGCGTGGTGG TCAAGGATTA TGTACCCGAT 
GATCTTTCCA ATTTCGCAGA GGCGCTAGCT AACCCTATTA AATACCAAAG TGAGCTAGAG 
AAGCGCGATA TTCAACTATC TGTTTTGACC GACGAAGCCG AAGCGCTGGG CAGTGCGGAC 
GAGGATCTCT CCAAGTCGTG TGGCCAAAAA AAGGAGCTGC GTCCGGTTGA ATCGCTTGCG 
ACTTCGCCGA AGCATAGGCC GAGCATCTCG GCCGCCGCAG CAATGAGTGT TGACGTTACC 
GTGGATCGGA CCGATGGCGG AAGGGGAGAA GATAGCATCT CTATTGTAGC ACCCTCAATA 
CAGCATCAGC ACTCTCTAGA CCAGTCCGTA AGCACCTCCA TCCGACAGGT GGAGTCCTCG 
CAGTTTGATG TCGATCTTGT GTTGGCGGAG CCGCTGGAAA AGATATTGGA TCACAAGTCC 
GTCAAGGAAA AGCGTCTCGA GATGGAAAAG AAGCTGGAGT CACTGCGAAA GAAGCATGAC 
AAGGAAAAGA TTAAGATCGC CGGCCAGAAA TCCAGTCCGC TAGAGGGCAA GAAGCCGAAG 
TTTGCCATCA CGAATAAGCT GGTGAAGCGG CTAAGCAACA AGAGTCTTAA TTGTCTTTCT 
CCGCACAGCG AGCCTGGTGT AGAGATCCCC GCCTGCCCTC TGGATCTGGG CGATAGCAGC 
GAGGAGAGTG CAGCCGCGGA TGCCGGTGAG GACTTAGCCG GTGGGAGCAG CAGTCTGGAT 
GGCAGAACTC AAGAGTCGCG ATTACGCAGT GCCTGCCGGG AGTACACTTC CCAGTACCGT 
GAGCTGCAGG AGAAGTACCA TGAGGCAATT TACAGTGCAG CCGAGAAGGT GCTGAAGACA 
TCGCAAACTG GGCAAACAAA GCAGTTAAAA GCATCTCTGG ACAAGGTCAC TGGAGAGGTT 
ATGCACCAGC TGCAGGAGGC CCGCCGCAAC GAGGTTAAGA ATCTGGCCAC CGTGCACCGG 
GATCGTGATG AGCTGATCAG AATGAAACGC GAGGTGGCAA GCTCCGTTGT GGAACGTGGC 
GTAGCTGAAC GTGTGCGGCT GAAGCAGACC TTTGACCGGA GAACGGATGA ACTTCAGAAG 
CAGCACGACT CAGTGCGCAA TGCCCTGGCA GAGCACCGCT CCAAGGCTCG TCAAATTCTC 
GACAAGGAGG CAGAATCGCG AAGCTGTGTA TCCAGCAATG GCTTCTTGGT CCTCTTCCAT 
GGACCTCATC ACCATGGCTG TACGGGCTCC GGCTCGTCAG CCCTTTCAGG CAATAATCTC 
ACTCTAAATC TGGACGCGGG GGCTGCCGGT AGTCATTCAG CCATTTCGCC GGCAAAGTCG 
CACAACAGCA TCGCAGCAGC GGCCGAGATG AAGACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plc21C ( NM_057503.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476851.2 Drosophila melanogaster phospholipase C at 21C, transcript variant B (Plc21C), mRNA.

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CloneID	OFa13332
Clone ID Related Accession (Same CDS sequence)	NM_057504.5 , NM_057503.5
Accession Version	NM_057503.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phospholipase C at 21C, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_057503.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGAGCG CCGGAGGAAC CTACATCTCC ACCGCCAGCG TGGAGGTGCC ACAAGCTCTG 
CAAGATGGCG AGAAGTTCAT TCGATGGGAT GATGACTCGG GCACTGGAAC ACCGGTTACA 
ATGCGCGTCG ATGCCAAGGG CTTCTTCCTC TACTGGGTCG ACCAGAACAA CGAGCTGGAT 
ATCCTTGATA TAGCCACCAT ACGCGACGTT CGCACAGGAC AGTACGCTAA GCGACCAAAG 
GACAACAAGC TGCGCCAGAT TGTGACGCTG GGCCCCCAGG ACACGCTCGA GGAGAAGACA 
GTGACGGTAT GCCACGGCTC GGACTTCGTC AACATGACGT TCGTCAACTT CTGCTGCACC 
CGGCGGGACA TAGCCCAGCT CTGGACCGAC GGACTCATCA AGTTGGCCTA CAGCTTGGCC 
CAGCTCAATG GCTCGGCGAT TATGTTCCTG CAGAAGGCCC ATACCAAACT GTGTCTCCAG 
GTGGACAAAA GCGGCCGCAT ACCAGTGAAA AACATCATAA AACTTTTTGC CCAAAACAAG 
GAGGATCGCA AACGTGTCGA GAAGGCGCTG GACGTGACCG GTTTGCCGTC GGGAAAGGTC 
GATAGCATAT CGGTGTCCAA ATTTCAATTC GAGGACTTTT ACAATTTGTA CAAATATCTC 
ACGCAGCGTT CGGAGGTGGA ACGCCTCTTT GACAGCATCG TTGGCAACTC GAAGCGCAAG 
TGCATGTCCA TCGCTCAACT GGTGGAGTTC CTGAACAAGA CACAGCGTGA TCCCCGCCTG 
AACGAGATCC TCTACCCTTA TGCGAATCCC GCCCGCGCCA AGGAGCTCAT CCAGCAATAT 
GAGCCCAACA AGTTCAATGC GCAAAAGGGC CAACTCAGCC TGGATGGCTT TTTGAGGTAC 
TTAATGGGCG ACGACAATCC CATCATGGCG CCCAGCAAGC TCGATCTCTG CGATGACATG 
GACCAGCCGA TGTCGCACTA CTTCATCAAC TCCTCGCATA ACACCTACTT GACGGGACAC 
CAGCTGACCG GCAAGTCGTC CGTCGAGATT TACAGGCAGT GTCTCCTGGC GGGATGCAGA 
TGCGTTGAGC TCGACTTCTG GAACGGACGC ACCGAGGAGC CAGTCATTGT CCACGGCTAT 
ACGTTCGTGC CCGAGATATT TGCCAAGGAC GTGCTGGAGG CCATCGCAGA GAGTGCATTT 
AAAACATCCG AATACCCTGT GATATTGAGC TTTGAGAATC ACTGCAACCC TAGGCAACAG 
GCGAAAATTG CCAACTATTG TCGCGAAATA TTTGGCGATA TGCTGCTCGA TAAGCCTCTG 
GACTCTCATC CGCTGGAGCC GAACATGGAC TTGCCACCGC CGGCGATGCT GCGCCGCAAG 
ATTATAATCA AGAACAAGAA GAAGCATCAC CACCACCATC ACCATCATCA CCACAAAAAG 
CCAGCGCAGG TGGGCACGCC GGCAGCCAAC AACAAACTGA CTACAGCGAA TTCAGTGGAT 
GCCAAGGCGG CGCAACAAGT CGGTTTGTCG GCCTCGCATG AGGATGGGGG CGTGACCAGG 
AGCACCGCCA ACGGTGACGT TGCCACTGGA ACAGGAACCG GAAGTGCTGC TGGGACAGCA 
GGACACGCGC CGCCGTTGCA ACAAATCCGC CAAAGCTCCA AAGACAGCAC TGGATCCTCC 
GATTCGGACA GCTCTTCGGA GGATGAATCC CTGCCTAACA CCACGCCCAA TTTGCCCAGC 
GGCAACGAAC CTCCGCCAGA AAAGGCTCAA AAGGAAACGG AGGCGGGAGC AGAGATTTCA 
GCTTTGGTCA ACTATGTTCA GCCCATCCAT TTTAGCTCTT TTGAGAATGC GGAAAAAAAG 
AATCGATGCT ACGAAATGTC TTCGTTTGAT GAAAAACAGG CGACAACATT ATTGAAGGAG 
CGACCGATTG AATTTGTGAA CTACAACAAA CACCAATTAT CTCGTGTCTA TCCGGCGGGC 
ACTCGCTTTG ATAGCTCCAA CTTTATGCCG CAGTTGTTCT GGAATGCCGG ATGTCAGCTC 
GTGGCACTTA ACTTCCAAAC CCTCGATCTG GCCATGCAAC TCAACTTGGG GATCTTTGAG 
TATAACGCTC GGTCCGGTTA TCTACTTAAG CCGGAGTTTA TGCGCCGATC AGATCGCAGA 
TTGGATCCCT TCGCGGAAAG CACTGTGGAT GGCATCATAG CAGGCACAGT ATCAATCACG 
GTTCTATCTG GTCAGTTTCT CACGGATAAG AGGGCGAACA CTTTTGTGGA AGTTGATATG 
TACGGTTTGC CAGCGGACAC GGTGAGAAAG AAGTTTCGCA CCAAGACAGT GCGGGATAAT 
GGAATGAATC CCCTTTATGA CGAGGAGCCG TTCGTGTTTA AGAAGGTGGT TCTCCCCGAG 
TTGGCGAGCA TTCGAATTGC AGCATATGAA GAGGGTGGCA AACTCATTGG TCACCGCGTT 
TTGCCCGTAA TCGGTCTTTG CCCTGGTTAC CGACATGTGA ATCTCCGATC TGAAGTGGGT 
CAACCAATAG CCCTTGCCTC TCTTTTCCTG TGCGTGGTGG TCAAGGATTA TGTACCCGAT 
GATCTTTCCA ATTTCGCAGA GGCGCTAGCT AACCCTATTA AATACCAAAG TGAGCTAGAG 
AAGCGCGATA TTCAACTATC TGTTTTGACC GACGAAGCCG AAGCGCTGGG CAGTGCGGAC 
GAGGATCTCT CCAAGTCGTG TGGCCAAAAA AAGGAGCTGC GTCCGGTTGA ATCGCTTGCG 
ACTTCGCCGA AGCATAGGCC GAGCATCTCG GCCGCCGCAG CAATGAGTGT TGACGTTACC 
GTGGATCGGA CCGATGGCGG AAGGGGAGAA GATAGCATCT CTATTGTAGC ACCCTCAATA 
CAGCATCAGC ACTCTCTAGA CCAGTCCGTA AGCACCTCCA TCCGACAGGT GGAGTCCTCG 
CAGTTTGATG TCGATCTTGT GTTGGCGGAG CCGCTGGAAA AGATATTGGA TCACAAGTCC 
GTCAAGGAAA AGCGTCTCGA GATGGAAAAG AAGCTGGAGT CACTGCGAAA GAAGCATGAC 
AAGGAAAAGA TTAAGATCGC CGGCCAGAAA TCCAGTCCGC TAGAGGGCAA GAAGCCGAAG 
TTTGCCATCA CGAATAAGCT GGTGAAGCGG CTAAGCAACA AGAGTCTTAA TTGTCTTTCT 
CCGCACAGCG AGCCTGGTGT AGAGATCCCC GCCTGCCCTC TGGATCTGGG CGATAGCAGC 
GAGGAGAGTG CAGCCGCGGA TGCCGGTGAG GACTTAGCCG GTGGGAGCAG CAGTCTGGAT 
GGCAGAACTC AAGAGTCGCG ATTACGCAGT GCCTGCCGGG AGTACACTTC CCAGTACCGT 
GAGCTGCAGG AGAAGTACCA TGAGGCAATT TACAGTGCAG CCGAGAAGGT GCTGAAGACA 
TCGCAAACTG GGCAAACAAA GCAGTTAAAA GCATCTCTGG ACAAGGTCAC TGGAGAGGTT 
ATGCACCAGC TGCAGGAGGC CCGCCGCAAC GAGGTTAAGA ATCTGGCCAC CGTGCACCGG 
GATCGTGATG AGCTGATCAG AATGAAACGC GAGGTGGCAA GCTCCGTTGT GGAACGTGGC 
GTAGCTGAAC GTGTGCGGCT GAAGCAGACC TTTGACCGGA GAACGGATGA ACTTCAGAAG 
CAGCACGACT CAGTGCGCAA TGCCCTGGCA GAGCACCGCT CCAAGGCTCG TCAAATTCTC 
GACAAGGAGG CAGAATCGCG AAGCTGTGTA TCCAGCAATG GCTTCTTGGT CCTCTTCCAT 
GGACCTCATC ACCATGGCTG TACGGGCTCC GGCTCGTCAG CCCTTTCAGG CAATAATCTC 
ACTCTAAATC TGGACGCGGG GGCTGCCGGT AGTCATTCAG CCATTTCGCC GGCAAAGTCG 
CACAACAGCA TCGCAGCAGC GGCCGAGATG AAGACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476851.2
CDS	692..4630
Translation	MMSAGGTYISTASVEVPQALQDGEKFIRWDDDSGTGTPVTMRVDAKGFFLYWVDQNNELDILDIATIRDVRTGQYAKRPKDNKLRQIVTLGPQDTLEEKTVTVCHGSDFVNMTFVNFCCTRRDIAQLWTDGLIKLAYSLAQLNGSAIMFLQKAHTKLCLQVDKSGRIPVKNIIKLFAQNKEDRKRVEKALDVTGLPSGKVDSISVSKFQFEDFYNLYKYLTQRSEVERLFDSIVGNSKRKCMSIAQLVEFLNKTQRDPRLNEILYPYANPARAKELIQQYEPNKFNAQKGQLSLDGFLRYLMGDDNPIMAPSKLDLCDDMDQPMSHYFINSSHNTYLTGHQLTGKSSVEIYRQCLLAGCRCVELDFWNGRTEEPVIVHGYTFVPEIFAKDVLEAIAESAFKTSEYPVILSFENHCNPRQQAKIANYCREIFGDMLLDKPLDSHPLEPNMDLPPPAMLRRKIIIKNKKKHHHHHHHHHHKKPAQVGTPAANNKLTTANSVDAKAAQQVGLSASHEDGGVTRSTANGDVATGTGTGSAAGTAGHAPPLQQIRQSSKDSTGSSDSDSSSEDESLPNTTPNLPSGNEPPPEKAQKETEAGAEISALVNYVQPIHFSSFENAEKKNRCYEMSSFDEKQATTLLKERPIEFVNYNKHQLSRVYPAGTRFDSSNFMPQLFWNAGCQLVALNFQTLDLAMQLNLGIFEYNARSGYLLKPEFMRRSDRRLDPFAESTVDGIIAGTVSITVLSGQFLTDKRANTFVEVDMYGLPADTVRKKFRTKTVRDNGMNPLYDEEPFVFKKVVLPELASIRIAAYEEGGKLIGHRVLPVIGLCPGYRHVNLRSEVGQPIALASLFLCVVVKDYVPDDLSNFAEALANPIKYQSELEKRDIQLSVLTDEAEALGSADEDLSKSCGQKKELRPVESLATSPKHRPSISAAAAMSVDVTVDRTDGGRGEDSISIVAPSIQHQHSLDQSVSTSIRQVESSQFDVDLVLAEPLEKILDHKSVKEKRLEMEKKLESLRKKHDKEKIKIAGQKSSPLEGKKPKFAITNKLVKRLSNKSLNCLSPHSEPGVEIPACPLDLGDSSEESAAADAGEDLAGGSSSLDGRTQESRLRSACREYTSQYRELQEKYHEAIYSAAEKVLKTSQTGQTKQLKASLDKVTGEVMHQLQEARRNEVKNLATVHRDRDELIRMKREVASSVVERGVAERVRLKQTFDRRTDELQKQHDSVRNALAEHRSKARQILDKEAESRSCVSSNGFLVLFHGPHHHGCTGSGSSALSGNNLTLNLDAGAAGSHSAISPAKSHNSIAAAAEMKT

Target ORF information:

RefSeq Version	NM_057503.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phospholipase C at 21C, transcript variant B (Plc21C), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057503.5

ORF Insert Sequence:

ATGATGAGCG CCGGAGGAAC CTACATCTCC ACCGCCAGCG TGGAGGTGCC ACAAGCTCTG 
CAAGATGGCG AGAAGTTCAT TCGATGGGAT GATGACTCGG GCACTGGAAC ACCGGTTACA 
ATGCGCGTCG ATGCCAAGGG CTTCTTCCTC TACTGGGTCG ACCAGAACAA CGAGCTGGAT 
ATCCTTGATA TAGCCACCAT ACGCGACGTT CGCACAGGAC AGTACGCTAA GCGACCAAAG 
GACAACAAGC TGCGCCAGAT TGTGACGCTG GGCCCCCAGG ACACGCTCGA GGAGAAGACA 
GTGACGGTAT GCCACGGCTC GGACTTCGTC AACATGACGT TCGTCAACTT CTGCTGCACC 
CGGCGGGACA TAGCCCAGCT CTGGACCGAC GGACTCATCA AGTTGGCCTA CAGCTTGGCC 
CAGCTCAATG GCTCGGCGAT TATGTTCCTG CAGAAGGCCC ATACCAAACT GTGTCTCCAG 
GTGGACAAAA GCGGCCGCAT ACCAGTGAAA AACATCATAA AACTTTTTGC CCAAAACAAG 
GAGGATCGCA AACGTGTCGA GAAGGCGCTG GACGTGACCG GTTTGCCGTC GGGAAAGGTC 
GATAGCATAT CGGTGTCCAA ATTTCAATTC GAGGACTTTT ACAATTTGTA CAAATATCTC 
ACGCAGCGTT CGGAGGTGGA ACGCCTCTTT GACAGCATCG TTGGCAACTC GAAGCGCAAG 
TGCATGTCCA TCGCTCAACT GGTGGAGTTC CTGAACAAGA CACAGCGTGA TCCCCGCCTG 
AACGAGATCC TCTACCCTTA TGCGAATCCC GCCCGCGCCA AGGAGCTCAT CCAGCAATAT 
GAGCCCAACA AGTTCAATGC GCAAAAGGGC CAACTCAGCC TGGATGGCTT TTTGAGGTAC 
TTAATGGGCG ACGACAATCC CATCATGGCG CCCAGCAAGC TCGATCTCTG CGATGACATG 
GACCAGCCGA TGTCGCACTA CTTCATCAAC TCCTCGCATA ACACCTACTT GACGGGACAC 
CAGCTGACCG GCAAGTCGTC CGTCGAGATT TACAGGCAGT GTCTCCTGGC GGGATGCAGA 
TGCGTTGAGC TCGACTTCTG GAACGGACGC ACCGAGGAGC CAGTCATTGT CCACGGCTAT 
ACGTTCGTGC CCGAGATATT TGCCAAGGAC GTGCTGGAGG CCATCGCAGA GAGTGCATTT 
AAAACATCCG AATACCCTGT GATATTGAGC TTTGAGAATC ACTGCAACCC TAGGCAACAG 
GCGAAAATTG CCAACTATTG TCGCGAAATA TTTGGCGATA TGCTGCTCGA TAAGCCTCTG 
GACTCTCATC CGCTGGAGCC GAACATGGAC TTGCCACCGC CGGCGATGCT GCGCCGCAAG 
ATTATAATCA AGAACAAGAA GAAGCATCAC CACCACCATC ACCATCATCA CCACAAAAAG 
CCAGCGCAGG TGGGCACGCC GGCAGCCAAC AACAAACTGA CTACAGCGAA TTCAGTGGAT 
GCCAAGGCGG CGCAACAAGT CGGTTTGTCG GCCTCGCATG AGGATGGGGG CGTGACCAGG 
AGCACCGCCA ACGGTGACGT TGCCACTGGA ACAGGAACCG GAAGTGCTGC TGGGACAGCA 
GGACACGCGC CGCCGTTGCA ACAAATCCGC CAAAGCTCCA AAGACAGCAC TGGATCCTCC 
GATTCGGACA GCTCTTCGGA GGATGAATCC CTGCCTAACA CCACGCCCAA TTTGCCCAGC 
GGCAACGAAC CTCCGCCAGA AAAGGCTCAA AAGGAAACGG AGGCGGGAGC AGAGATTTCA 
GCTTTGGTCA ACTATGTTCA GCCCATCCAT TTTAGCTCTT TTGAGAATGC GGAAAAAAAG 
AATCGATGCT ACGAAATGTC TTCGTTTGAT GAAAAACAGG CGACAACATT ATTGAAGGAG 
CGACCGATTG AATTTGTGAA CTACAACAAA CACCAATTAT CTCGTGTCTA TCCGGCGGGC 
ACTCGCTTTG ATAGCTCCAA CTTTATGCCG CAGTTGTTCT GGAATGCCGG ATGTCAGCTC 
GTGGCACTTA ACTTCCAAAC CCTCGATCTG GCCATGCAAC TCAACTTGGG GATCTTTGAG 
TATAACGCTC GGTCCGGTTA TCTACTTAAG CCGGAGTTTA TGCGCCGATC AGATCGCAGA 
TTGGATCCCT TCGCGGAAAG CACTGTGGAT GGCATCATAG CAGGCACAGT ATCAATCACG 
GTTCTATCTG GTCAGTTTCT CACGGATAAG AGGGCGAACA CTTTTGTGGA AGTTGATATG 
TACGGTTTGC CAGCGGACAC GGTGAGAAAG AAGTTTCGCA CCAAGACAGT GCGGGATAAT 
GGAATGAATC CCCTTTATGA CGAGGAGCCG TTCGTGTTTA AGAAGGTGGT TCTCCCCGAG 
TTGGCGAGCA TTCGAATTGC AGCATATGAA GAGGGTGGCA AACTCATTGG TCACCGCGTT 
TTGCCCGTAA TCGGTCTTTG CCCTGGTTAC CGACATGTGA ATCTCCGATC TGAAGTGGGT 
CAACCAATAG CCCTTGCCTC TCTTTTCCTG TGCGTGGTGG TCAAGGATTA TGTACCCGAT 
GATCTTTCCA ATTTCGCAGA GGCGCTAGCT AACCCTATTA AATACCAAAG TGAGCTAGAG 
AAGCGCGATA TTCAACTATC TGTTTTGACC GACGAAGCCG AAGCGCTGGG CAGTGCGGAC 
GAGGATCTCT CCAAGTCGTG TGGCCAAAAA AAGGAGCTGC GTCCGGTTGA ATCGCTTGCG 
ACTTCGCCGA AGCATAGGCC GAGCATCTCG GCCGCCGCAG CAATGAGTGT TGACGTTACC 
GTGGATCGGA CCGATGGCGG AAGGGGAGAA GATAGCATCT CTATTGTAGC ACCCTCAATA 
CAGCATCAGC ACTCTCTAGA CCAGTCCGTA AGCACCTCCA TCCGACAGGT GGAGTCCTCG 
CAGTTTGATG TCGATCTTGT GTTGGCGGAG CCGCTGGAAA AGATATTGGA TCACAAGTCC 
GTCAAGGAAA AGCGTCTCGA GATGGAAAAG AAGCTGGAGT CACTGCGAAA GAAGCATGAC 
AAGGAAAAGA TTAAGATCGC CGGCCAGAAA TCCAGTCCGC TAGAGGGCAA GAAGCCGAAG 
TTTGCCATCA CGAATAAGCT GGTGAAGCGG CTAAGCAACA AGAGTCTTAA TTGTCTTTCT 
CCGCACAGCG AGCCTGGTGT AGAGATCCCC GCCTGCCCTC TGGATCTGGG CGATAGCAGC 
GAGGAGAGTG CAGCCGCGGA TGCCGGTGAG GACTTAGCCG GTGGGAGCAG CAGTCTGGAT 
GGCAGAACTC AAGAGTCGCG ATTACGCAGT GCCTGCCGGG AGTACACTTC CCAGTACCGT 
GAGCTGCAGG AGAAGTACCA TGAGGCAATT TACAGTGCAG CCGAGAAGGT GCTGAAGACA 
TCGCAAACTG GGCAAACAAA GCAGTTAAAA GCATCTCTGG ACAAGGTCAC TGGAGAGGTT 
ATGCACCAGC TGCAGGAGGC CCGCCGCAAC GAGGTTAAGA ATCTGGCCAC CGTGCACCGG 
GATCGTGATG AGCTGATCAG AATGAAACGC GAGGTGGCAA GCTCCGTTGT GGAACGTGGC 
GTAGCTGAAC GTGTGCGGCT GAAGCAGACC TTTGACCGGA GAACGGATGA ACTTCAGAAG 
CAGCACGACT CAGTGCGCAA TGCCCTGGCA GAGCACCGCT CCAAGGCTCG TCAAATTCTC 
GACAAGGAGG CAGAATCGCG AAGCTGTGTA TCCAGCAATG GCTTCTTGGT CCTCTTCCAT 
GGACCTCATC ACCATGGCTG TACGGGCTCC GGCTCGTCAG CCCTTTCAGG CAATAATCTC 
ACTCTAAATC TGGACGCGGG GGCTGCCGGT AGTCATTCAG CCATTTCGCC GGCAAAGTCG 
CACAACAGCA TCGCAGCAGC GGCCGAGATG AAGACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plc21C ( NM_205883.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995605.1 Drosophila melanogaster phospholipase C at 21C, transcript variant D (Plc21C), mRNA.

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CloneID	OFa13333
Clone ID Related Accession (Same CDS sequence)	NM_205883.2
Accession Version	NM_205883.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3957bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phospholipase C at 21C, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_205883.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGAGCG CCGGAGGAAC CTACATCTCC ACCGCCAGCG TGGAGGTGCC ACAAGCTCTG 
CAAGATGGCG AGAAGTTCAT TCGATGGGAT GATGACTCGG GCACTGGAAC ACCGGTTACA 
ATGCGCGTCG ATGCCAAGGG CTTCTTCCTC TACTGGGTCG ACCAGAACAA CGAGCTGGAT 
ATCCTTGATA TAGCCACCAT ACGCGACGTT CGCACAGGAC AGTACGCTAA GCGACCAAAG 
GACAACAAGC TGCGCCAGAT TGTGACGCTG GGCCCCCAGG ACACGCTCGA GGAGAAGACA 
GTGACGGTAT GCCACGGCTC GGACTTCGTC AACATGACGT TCGTCAACTT CTGCTGCACC 
CGGCGGGACA TAGCCCAGCT CTGGACCGAC GGACTCATCA AGTTGGCCTA CAGCTTGGCC 
CAGCTCAATG GCTCGGCGAT TATGTTCCTG CAGAAGGCCC ATACCAAACT GTGTCTCCAG 
GTGGACAAAA GCGGCCGCAT ACCAGTGAAA AACATCATAA AACTTTTTGC CCAAAACAAG 
GAGGATCGCA AACGTGTCGA GAAGGCGCTG GACGTGACCG GTTTGCCGTC GGGAAAGGTC 
GATAGCATAT CGGTGTCCAA ATTTCAATTC GAGGACTTTT ACAATTTGTA CAAATATCTC 
ACGCAGCGTT CGGAGGTGGA ACGCCTCTTT GACAGCATCG TTGGCAACTC GAAGCGCAAG 
TGCATGTCCA TCGCTCAACT GGTGGAGTTC CTGAACAAGA CACAGCGTGA TCCCCGCCTG 
AACGAGATCC TCTACCCTTA TGCGAATCCC GCCCGCGCCA AGGAGCTCAT CCAGCAATAT 
GAGCCCAACA AGTTCAATGC GCAAAAGGGC CAACTCAGCC TGGATGGCTT TTTGAGGTAC 
TTAATGGGCG ACGACAATCC CATCATGGCG CCCAGCAAGC TCGATCTCTG CGATGACATG 
GACCAGCCGA TGTCGCACTA CTTCATCAAC TCCTCGCATA ACACCTACTT GACGGGACAC 
CAGCTGACCG GCAAGTCGTC CGTCGAGATT TACAGGCAGT GTCTCCTGGC GGGATGCAGA 
TGCGTTGAGC TCGACTTCTG GAACGGACGC ACCGAGGAGC CAGTCATTGT CCACGGCTAT 
ACGTTCGTGC CCGAGATATT TGCCAAGGAC GTGCTGGAGG CCATCGCAGA GAGTGCATTT 
AAAACATCCG AATACCCTGT GATATTGAGC TTTGAGAATC ACTGCAACCC TAGGCAACAG 
GCGAAAATTG CCAACTATTG TCGCGAAATA TTTGGCGATA TGCTGCTCGA TAAGCCTCTG 
GACTCTCATC CGCTGGAGCC GAACATGGAC TTGCCACCGC CGGCGATGCT GCGCCGCAAG 
ATTATAATCA AGAACAAGAA GAAGCATCAC CACCACCATC ACCATCATCA CCACAAAAAG 
CCAGCGCAGG TGGGCACGCC GGCAGCCAAC AACAAACTGA CTACAGCGAA TTCAGTGGAT 
GCCAAGGCGG CGCAACAAGT CGGTTTGTCG GCCTCGCATG AGGATGGGGG CGTGACCAGG 
AGCACCGCCA ACGGTGACGT TGCCACTGGA ACAGGAACCG GAAGTGCTGC TGGGACAGCA 
GGACACGCGC CGCCGTTGCA ACAAATCCGC CAAAGCTCCA AAGACAGCAC TGGATCCTCC 
GATTCGGACA GCTCTTCGGA GGATGAATCC CTGCCTAACA CCACGCCCAA TTTGCCCAGC 
GGCAACGAAC CTCCGCCAGA AAAGGCTCAA AAGGAAACGG AGGCGGGAGC AGAGATTTCA 
GCTTTGGTCA ACTATGTTCA GCCCATCCAT TTTAGCTCTT TTGAGAATGC GGAAAAAAAG 
AATCGATGCT ACGAAATGTC TTCGTTTGAT GAAAAACAGG CGACAACATT ATTGAAGGAG 
CGACCGATTG AATTTGTGAA CTACAACAAA CACCAATTAT CTCGTGTCTA TCCGGCGGGC 
ACTCGCTTTG ATAGCTCCAA CTTTATGCCG CAGTTGTTCT GGAATGCCGG ATGTCAGCTC 
GTGGCACTTA ACTTCCAAAC CCTCGATCTG GCCATGCAAC TCAACTTGGG GATCTTTGAG 
TATAACGCTC GGTCCGGTTA TCTACTTAAG CCGGAGTTTA TGCGCCGATC AGATCGCAGA 
TTGGATCCCT TCGCGGAAAG CACTGTGGAT GGCATCATAG CAGGCACAGT ATCAATCACG 
GTTCTATCTG GTCAGTTTCT CACGGATAAG AGGGCGAACA CTTTTGTGGA AGTTGATATG 
TACGGTTTGC CAGCGGACAC GGTGAGAAAG AAGTTTCGCA CCAAGACAGT GCGGGATAAT 
GGAATGAATC CCCTTTATGA CGAGGAGCCG TTCGTGTTTA AGAAGGTGGT TCTCCCCGAG 
TTGGCGAGCA TTCGAATTGC AGCATATGAA GAGGGTGGCA AACTCATTGG TCACCGCGTT 
TTGCCCGTAA TCGGTCTTTG CCCTGGTTAC CGACATGTGA ATCTCCGATC TGAAGTGGGT 
CAACCAATAG CCCTTGCCTC TCTTTTCCTG TGCGTGGTGG TCAAGGATTA TGTACCCGAT 
GATCTTTCCA ATTTCGCAGA GGCGCTAGCT AACCCTATTA AATACCAAAG TGAGCTAGAG 
AAGCGCGATA TTCAACTATC TGTTTTGACC GACGAAGCCG AAGCGCTGGG CAGTGCGGAC 
GAGGATCTCT CCAAGTCGTT CGTTTTCGTC CAAGTAGGTG GCCAAAAAAA GGAGCTGCGT 
CCGGTTGAAT CGCTTGCGAC TTCGCCGAAG CATAGGCCGA GCATCTCGGC CGCCGCAGCA 
ATGAGTGTTG ACGTTACCGT GGATCGGACC GATGGCGGAA GGGGAGAAGA TAGCATCTCT 
ATTGTAGCAC CCTCAATACA GCATCAGCAC TCTCTAGACC AGTCCGTAAG CACCTCCATC 
CGACAGGTGG AGTCCTCGCA GTTTGATGTC GATCTTGTGT TGGCGGAGCC GCTGGAAAAG 
ATATTGGATC ACAAGTCCGT CAAGGAAAAG CGTCTCGAGA TGGAAAAGAA GCTGGAGTCA 
CTGCGAAAGA AGCATGACAA GGAAAAGATT AAGATCGCCG GCCAGAAATC CAGTCCGCTA 
GAGGGCAAGA AGCCGAAGTT TGCCATCACG AATAAGCTGG TGAAGCGGCT AAGCAACAAG 
AGTCTTAATT GTCTTTCTCC GCACAGCGAG CCTGGTGTAG AGATCCCCGC CTGCCCTCTG 
GATCTGGGCG ATAGCAGCGA GGAGAGTGCA GCCGCGGATG CCGGTGAGGA CTTAGCCGGT 
GGGAGCAGCA GTCTGGATGG CAGAACTCAA GAGTCGCGAT TACGCAGTGC CTGCCGGGAG 
TACACTTCCC AGTACCGTGA GCTGCAGGAG AAGTACCATG AGGCAATTTA CAGTGCAGCC 
GAGAAGGTGC TGAAGACATC GCAAACTGGG CAAACAAAGC AGTTAAAAGC ATCTCTGGAC 
AAGGTCACTG GAGAGGTTAT GCACCAGCTG CAGGAGGCCC GCCGCAACGA GGTTAAGAAT 
CTGGCCACCG TGCACCGGGA TCGTGATGAG CTGATCAGAA TGAAACGCGA GGTGGCAAGC 
TCCGTTGTGG AACGTGGCGT AGCTGAACGT GTGCGGCTGA AGCAGACCTT TGACCGGAGA 
ACGGATGAAC TTCAGAAGCA GCACGACTCA GTGCGCAATG CCCTGGCAGA GCACCGCTCC 
AAGGCTCGTC AAATTCTCGA CAAGGAGGCA GAATCGCGAA GCTGTGTATC CAGCAATGGC 
TTCTTGGTCC TCTTCCATGG ACCTCATCAC CATGGCTGTA CGGGCTCCGG CTCGTCAGCC 
CTTTCAGGCA ATAATCTCAC TCTAAATCTG GACGCGGGGG CTGCCGGTAG TCATTCAGCC 
ATTTCGCCGG CAAAGTCGCA CAACAGCATC GCAGCAGCGG CCGAGATGAA GACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995605.1
CDS	692..4648
Translation	MMSAGGTYISTASVEVPQALQDGEKFIRWDDDSGTGTPVTMRVDAKGFFLYWVDQNNELDILDIATIRDVRTGQYAKRPKDNKLRQIVTLGPQDTLEEKTVTVCHGSDFVNMTFVNFCCTRRDIAQLWTDGLIKLAYSLAQLNGSAIMFLQKAHTKLCLQVDKSGRIPVKNIIKLFAQNKEDRKRVEKALDVTGLPSGKVDSISVSKFQFEDFYNLYKYLTQRSEVERLFDSIVGNSKRKCMSIAQLVEFLNKTQRDPRLNEILYPYANPARAKELIQQYEPNKFNAQKGQLSLDGFLRYLMGDDNPIMAPSKLDLCDDMDQPMSHYFINSSHNTYLTGHQLTGKSSVEIYRQCLLAGCRCVELDFWNGRTEEPVIVHGYTFVPEIFAKDVLEAIAESAFKTSEYPVILSFENHCNPRQQAKIANYCREIFGDMLLDKPLDSHPLEPNMDLPPPAMLRRKIIIKNKKKHHHHHHHHHHKKPAQVGTPAANNKLTTANSVDAKAAQQVGLSASHEDGGVTRSTANGDVATGTGTGSAAGTAGHAPPLQQIRQSSKDSTGSSDSDSSSEDESLPNTTPNLPSGNEPPPEKAQKETEAGAEISALVNYVQPIHFSSFENAEKKNRCYEMSSFDEKQATTLLKERPIEFVNYNKHQLSRVYPAGTRFDSSNFMPQLFWNAGCQLVALNFQTLDLAMQLNLGIFEYNARSGYLLKPEFMRRSDRRLDPFAESTVDGIIAGTVSITVLSGQFLTDKRANTFVEVDMYGLPADTVRKKFRTKTVRDNGMNPLYDEEPFVFKKVVLPELASIRIAAYEEGGKLIGHRVLPVIGLCPGYRHVNLRSEVGQPIALASLFLCVVVKDYVPDDLSNFAEALANPIKYQSELEKRDIQLSVLTDEAEALGSADEDLSKSFVFVQVGGQKKELRPVESLATSPKHRPSISAAAAMSVDVTVDRTDGGRGEDSISIVAPSIQHQHSLDQSVSTSIRQVESSQFDVDLVLAEPLEKILDHKSVKEKRLEMEKKLESLRKKHDKEKIKIAGQKSSPLEGKKPKFAITNKLVKRLSNKSLNCLSPHSEPGVEIPACPLDLGDSSEESAAADAGEDLAGGSSSLDGRTQESRLRSACREYTSQYRELQEKYHEAIYSAAEKVLKTSQTGQTKQLKASLDKVTGEVMHQLQEARRNEVKNLATVHRDRDELIRMKREVASSVVERGVAERVRLKQTFDRRTDELQKQHDSVRNALAEHRSKARQILDKEAESRSCVSSNGFLVLFHGPHHHGCTGSGSSALSGNNLTLNLDAGAAGSHSAISPAKSHNSIAAAAEMKT

Target ORF information:

RefSeq Version	NM_205883.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phospholipase C at 21C, transcript variant D (Plc21C), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205883.2

ORF Insert Sequence:

ATGATGAGCG CCGGAGGAAC CTACATCTCC ACCGCCAGCG TGGAGGTGCC ACAAGCTCTG 
CAAGATGGCG AGAAGTTCAT TCGATGGGAT GATGACTCGG GCACTGGAAC ACCGGTTACA 
ATGCGCGTCG ATGCCAAGGG CTTCTTCCTC TACTGGGTCG ACCAGAACAA CGAGCTGGAT 
ATCCTTGATA TAGCCACCAT ACGCGACGTT CGCACAGGAC AGTACGCTAA GCGACCAAAG 
GACAACAAGC TGCGCCAGAT TGTGACGCTG GGCCCCCAGG ACACGCTCGA GGAGAAGACA 
GTGACGGTAT GCCACGGCTC GGACTTCGTC AACATGACGT TCGTCAACTT CTGCTGCACC 
CGGCGGGACA TAGCCCAGCT CTGGACCGAC GGACTCATCA AGTTGGCCTA CAGCTTGGCC 
CAGCTCAATG GCTCGGCGAT TATGTTCCTG CAGAAGGCCC ATACCAAACT GTGTCTCCAG 
GTGGACAAAA GCGGCCGCAT ACCAGTGAAA AACATCATAA AACTTTTTGC CCAAAACAAG 
GAGGATCGCA AACGTGTCGA GAAGGCGCTG GACGTGACCG GTTTGCCGTC GGGAAAGGTC 
GATAGCATAT CGGTGTCCAA ATTTCAATTC GAGGACTTTT ACAATTTGTA CAAATATCTC 
ACGCAGCGTT CGGAGGTGGA ACGCCTCTTT GACAGCATCG TTGGCAACTC GAAGCGCAAG 
TGCATGTCCA TCGCTCAACT GGTGGAGTTC CTGAACAAGA CACAGCGTGA TCCCCGCCTG 
AACGAGATCC TCTACCCTTA TGCGAATCCC GCCCGCGCCA AGGAGCTCAT CCAGCAATAT 
GAGCCCAACA AGTTCAATGC GCAAAAGGGC CAACTCAGCC TGGATGGCTT TTTGAGGTAC 
TTAATGGGCG ACGACAATCC CATCATGGCG CCCAGCAAGC TCGATCTCTG CGATGACATG 
GACCAGCCGA TGTCGCACTA CTTCATCAAC TCCTCGCATA ACACCTACTT GACGGGACAC 
CAGCTGACCG GCAAGTCGTC CGTCGAGATT TACAGGCAGT GTCTCCTGGC GGGATGCAGA 
TGCGTTGAGC TCGACTTCTG GAACGGACGC ACCGAGGAGC CAGTCATTGT CCACGGCTAT 
ACGTTCGTGC CCGAGATATT TGCCAAGGAC GTGCTGGAGG CCATCGCAGA GAGTGCATTT 
AAAACATCCG AATACCCTGT GATATTGAGC TTTGAGAATC ACTGCAACCC TAGGCAACAG 
GCGAAAATTG CCAACTATTG TCGCGAAATA TTTGGCGATA TGCTGCTCGA TAAGCCTCTG 
GACTCTCATC CGCTGGAGCC GAACATGGAC TTGCCACCGC CGGCGATGCT GCGCCGCAAG 
ATTATAATCA AGAACAAGAA GAAGCATCAC CACCACCATC ACCATCATCA CCACAAAAAG 
CCAGCGCAGG TGGGCACGCC GGCAGCCAAC AACAAACTGA CTACAGCGAA TTCAGTGGAT 
GCCAAGGCGG CGCAACAAGT CGGTTTGTCG GCCTCGCATG AGGATGGGGG CGTGACCAGG 
AGCACCGCCA ACGGTGACGT TGCCACTGGA ACAGGAACCG GAAGTGCTGC TGGGACAGCA 
GGACACGCGC CGCCGTTGCA ACAAATCCGC CAAAGCTCCA AAGACAGCAC TGGATCCTCC 
GATTCGGACA GCTCTTCGGA GGATGAATCC CTGCCTAACA CCACGCCCAA TTTGCCCAGC 
GGCAACGAAC CTCCGCCAGA AAAGGCTCAA AAGGAAACGG AGGCGGGAGC AGAGATTTCA 
GCTTTGGTCA ACTATGTTCA GCCCATCCAT TTTAGCTCTT TTGAGAATGC GGAAAAAAAG 
AATCGATGCT ACGAAATGTC TTCGTTTGAT GAAAAACAGG CGACAACATT ATTGAAGGAG 
CGACCGATTG AATTTGTGAA CTACAACAAA CACCAATTAT CTCGTGTCTA TCCGGCGGGC 
ACTCGCTTTG ATAGCTCCAA CTTTATGCCG CAGTTGTTCT GGAATGCCGG ATGTCAGCTC 
GTGGCACTTA ACTTCCAAAC CCTCGATCTG GCCATGCAAC TCAACTTGGG GATCTTTGAG 
TATAACGCTC GGTCCGGTTA TCTACTTAAG CCGGAGTTTA TGCGCCGATC AGATCGCAGA 
TTGGATCCCT TCGCGGAAAG CACTGTGGAT GGCATCATAG CAGGCACAGT ATCAATCACG 
GTTCTATCTG GTCAGTTTCT CACGGATAAG AGGGCGAACA CTTTTGTGGA AGTTGATATG 
TACGGTTTGC CAGCGGACAC GGTGAGAAAG AAGTTTCGCA CCAAGACAGT GCGGGATAAT 
GGAATGAATC CCCTTTATGA CGAGGAGCCG TTCGTGTTTA AGAAGGTGGT TCTCCCCGAG 
TTGGCGAGCA TTCGAATTGC AGCATATGAA GAGGGTGGCA AACTCATTGG TCACCGCGTT 
TTGCCCGTAA TCGGTCTTTG CCCTGGTTAC CGACATGTGA ATCTCCGATC TGAAGTGGGT 
CAACCAATAG CCCTTGCCTC TCTTTTCCTG TGCGTGGTGG TCAAGGATTA TGTACCCGAT 
GATCTTTCCA ATTTCGCAGA GGCGCTAGCT AACCCTATTA AATACCAAAG TGAGCTAGAG 
AAGCGCGATA TTCAACTATC TGTTTTGACC GACGAAGCCG AAGCGCTGGG CAGTGCGGAC 
GAGGATCTCT CCAAGTCGTT CGTTTTCGTC CAAGTAGGTG GCCAAAAAAA GGAGCTGCGT 
CCGGTTGAAT CGCTTGCGAC TTCGCCGAAG CATAGGCCGA GCATCTCGGC CGCCGCAGCA 
ATGAGTGTTG ACGTTACCGT GGATCGGACC GATGGCGGAA GGGGAGAAGA TAGCATCTCT 
ATTGTAGCAC CCTCAATACA GCATCAGCAC TCTCTAGACC AGTCCGTAAG CACCTCCATC 
CGACAGGTGG AGTCCTCGCA GTTTGATGTC GATCTTGTGT TGGCGGAGCC GCTGGAAAAG 
ATATTGGATC ACAAGTCCGT CAAGGAAAAG CGTCTCGAGA TGGAAAAGAA GCTGGAGTCA 
CTGCGAAAGA AGCATGACAA GGAAAAGATT AAGATCGCCG GCCAGAAATC CAGTCCGCTA 
GAGGGCAAGA AGCCGAAGTT TGCCATCACG AATAAGCTGG TGAAGCGGCT AAGCAACAAG 
AGTCTTAATT GTCTTTCTCC GCACAGCGAG CCTGGTGTAG AGATCCCCGC CTGCCCTCTG 
GATCTGGGCG ATAGCAGCGA GGAGAGTGCA GCCGCGGATG CCGGTGAGGA CTTAGCCGGT 
GGGAGCAGCA GTCTGGATGG CAGAACTCAA GAGTCGCGAT TACGCAGTGC CTGCCGGGAG 
TACACTTCCC AGTACCGTGA GCTGCAGGAG AAGTACCATG AGGCAATTTA CAGTGCAGCC 
GAGAAGGTGC TGAAGACATC GCAAACTGGG CAAACAAAGC AGTTAAAAGC ATCTCTGGAC 
AAGGTCACTG GAGAGGTTAT GCACCAGCTG CAGGAGGCCC GCCGCAACGA GGTTAAGAAT 
CTGGCCACCG TGCACCGGGA TCGTGATGAG CTGATCAGAA TGAAACGCGA GGTGGCAAGC 
TCCGTTGTGG AACGTGGCGT AGCTGAACGT GTGCGGCTGA AGCAGACCTT TGACCGGAGA 
ACGGATGAAC TTCAGAAGCA GCACGACTCA GTGCGCAATG CCCTGGCAGA GCACCGCTCC 
AAGGCTCGTC AAATTCTCGA CAAGGAGGCA GAATCGCGAA GCTGTGTATC CAGCAATGGC 
TTCTTGGTCC TCTTCCATGG ACCTCATCAC CATGGCTGTA CGGGCTCCGG CTCGTCAGCC 
CTTTCAGGCA ATAATCTCAC TCTAAATCTG GACGCGGGGG CTGCCGGTAG TCATTCAGCC 
ATTTCGCCGG CAAAGTCGCA CAACAGCATC GCAGCAGCGG CCGAGATGAA GACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plc21C ( NM_205882.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995604.2 Drosophila melanogaster phospholipase C at 21C, transcript variant H (Plc21C), mRNA.

Price & Availability↑

CloneID	OFa13334
Clone ID Related Accession (Same CDS sequence)	NM_205882.2
Accession Version	NM_205882.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3921bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phospholipase C at 21C, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_205882.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGAGCG CCGGAGGAAC CTACATCTCC ACCGCCAGCG TGGAGGTGCC ACAAGCTCTG 
CAAGATGGCG AGAAGTTCAT TCGATGGGAT GATGACTCGG GCACTGGAAC ACCGGTTACA 
ATGCGCGTCG ATGCCAAGGG CTTCTTCCTC TACTGGGTCG ACCAGAACAA CGAGCTGGAT 
ATCCTTGATA TAGCCACCAT ACGCGACGTT CGCACAGGAC AGTACGCTAA GCGACCAAAG 
GACAACAAGC TGCGCCAGAT TGTGACGCTG GGCCCCCAGG ACACGCTCGA GGAGAAGACA 
GTGACGGTAT GCCACGGCTC GGACTTCGTC AACATGACGT TCGTCAACTT CTGCTGCACC 
CGGCGGGACA TAGCCCAGCT CTGGACCGAC GGACTCATCA AGTTGGCCTA CAGCTTGGCC 
CAGCTCAATG GCTCGGCGAT TATGTTCCTG CAGAAGGCCC ATACCAAACT GTGTCTCCAG 
GTGGACAAAA GCGGCCGCAT ACCAGTGAAA AACATCATAA AACTTTTTGC CCAAAACAAG 
GAGGATCGCA AACGTGTCGA GAAGGCGCTG GACGTGACCG GTTTGCCGTC GGGAAAGGTC 
GATAGCATAT CGGTGTCCAA ATTTCAATTC GAGGACTTTT ACAATTTGTA CAAATATCTC 
ACGCAGCGTT CGGAGGTGGA ACGCCTCTTT GACAGCATCG TTGGCAACTC GAAGCGCAAG 
TGCATGTCCA TCGCTCAACT GGTGGAGTTC CTGAACAAGA CACAGCGTGA TCCCCGCCTG 
AACGAGATCC TCTACCCTTA TGCGAATCCC GCCCGCGCCA AGGAGCTCAT CCAGCAATAT 
GAGCCCAACA AGTTCAATGC GCAAAAGGGC CAACTCAGCC TGGATGGCTT TTTGAGGTAC 
TTAATGGGCG ACGACAATCC CATCATGGCG CCCAGCAAGC TCGATCTCTG CGATGACATG 
GACCAGCCGA TGTCGCACTA CTTCATCAAC TCCTCGCATA ACACCTACTT GACGGGACAC 
CAGCTGACCG GCAAGTCGTC CGTCGAGATT TACAGGCAGT GTCTCCTGGC GGGATGCAGA 
TGCGTTGAGC TCGACTTCTG GAACGGACGC ACCGAGGAGC CAGTCATTGT CCACGGCTAT 
ACGTTCGTGC CCGAGATATT TGCCAAGGAC GTGCTGGAGG CCATCGCAGA GAGTGCATTT 
AAAACATCCG AATACCCTGT GATATTGAGC TTTGAGAATC ACTGCAACCC TAGGCAACAG 
GCGAAAATTG CCAACTATTG TCGCGAAATA TTTGGCGATA TGCTGCTCGA TAAGCCTCTG 
GACTCTCATC CGCTGGAGCC GAACATGGAC TTGCCACCGC CGGCGATGCT GCGCCGCAAG 
ATTATAATCA AGAACAAGAA GAAGCATCAC CACCACCATC ACCATCATCA CCACAAAAAG 
CCAGCGCAGG TGGGCACGCC GGCAGCCAAC AACAAACTGA CTACAGCGAA TTCAGTGGAT 
GCCAAGGCGG CGCAACAAGT CGGTTTGTCG GCCTCGCATG AGGATGGGGG CGTGACCAGG 
AGCACCGCCA ACGGTGACGT TGCCACTGGA ACAGGAACCG GAAGTGCTGC TGGGACAGCA 
GGACACGCGC CGCCGTTGCA ACAAATCCGC CAAAGCTCCA AAGACAGCAC TGGATCCTCC 
GATTCGGACA GCTCTTCGGA GGATGAATCC CTGCCTAACA CCACGCCCAA TTTGCCCAGC 
GGCAACGAAC CTCCGCCAGA AAAGGCTCAA AAGGAAACGG AGGCGGGAGC AGAGATTTCA 
GCTTTGGTCA ACTATGTTCA GCCCATCCAT TTTAGCTCTT TTGAGAATGC GGAAAAAAAG 
AATCGATGCT ACGAAATGTC TTCGTTTGAT GAAAAACAGG CGACAACATT ATTGAAGGAG 
CGACCGATTG AATTTGTGAA CTACAACAAA CACCAATTAT CTCGTGTCTA TCCGGCGGGC 
ACTCGCTTTG ATAGCTCCAA CTTTATGCCG CAGTTGTTCT GGAATGCCGG ATGTCAGCTC 
GTGGCACTTA ACTTCCAAAC CCTCGATCTG GCCATGCAAC TCAACTTGGG GATCTTTGAG 
TATAACGCTC GGTCCGGTTA TCTACTTAAG CCGGAGTTTA TGCGCCGATC AGATCGCAGA 
TTGGATCCCT TCGCGGAAAG CACTGTGGAT GGCATCATAG CAGGCACAGT ATCAATCACG 
GTTCTATCTG GTCAGTTTCT CACGGATAAG AGGGCGAACA CTTTTGTGGA AGTTGATATG 
TACGGTTTGC CAGCGGACAC GGTGAGAAAG AAGTTTCGCA CCAAGACAGT GCGGGATAAT 
GGAATGAATC CCCTTTATGA CGAGGAGCCG TTCGTGTTTA AGAAGGTGGT TCTCCCCGAG 
TTGGCGAGCA TTCGAATTGC AGCATATGAA GAGGGTGGCA AACTCATTGG TCACCGCGTT 
TTGCCCGTAA TCGGTCTTTG CCCTGGTTAC CGACATGTGA ATCTCCGATC TGAAGTGGGT 
CAACCAATAG CCCTTGCCTC TCTTTTCCTG TGCGTGGTGG TCAAGGATTA TGTACCCGAT 
GATCTTTCCA ATTTCGCAGA GGCGCTAGCT AACCCTATTA AATACCAAAG TGAGCTAGAG 
AAGCGCGATA TTCAACTATC TGTTTTGACC GACGAAGCCG AAGCGCTGGG CAGTGCGGAC 
GAGGATCTCT CCAAGTCGTT AGGTGGCCAA AAAAAGGAGC TGCGTCCGGT TGAATCGCTT 
GCGACTTCGC CGAAGCATAG GCCGAGCATC TCGGCCGCCG CAGCAATGAG TGTTGACGTT 
ACCGTGGATC GGACCGATGG CGGAAGGGGA GAAGATAGCA TCTCTATTGT AGCACCCTCA 
ATACAGCATC AGCACTCTCT AGACCAGTCC GTAAGCACCT CCATCCGACA GGTGGAGTCC 
TCGCAGTTTG ATGTCGATCT TGTGTTGGCG GAGCCGCTGG AAAAGATATT GGATCACAAG 
TCCGTCAAGG AAAAGCGTCT CGAGATGGAA AAGAAGCTGG AGTCACTGCG AAAGAAGCAT 
GACAAGGAAA AGATTAAGAT CGCCGGCCAG AAATCCAGTC CGCTAGAGGG CAAGAAGCCG 
AAGTTTGCCA TCACGAATAA GCTGGTGAAG CGGCTAAGCA ACAAGAGTCT CGAGCCTGGT 
GTAGAGATCC CCGCCTGCCC TCTGGATCTG GGCGATAGCA GCGAGGAGAG TGCAGCCGCG 
GATGCCGGTG AGGACTTAGC CGGTGGGAGC AGCAGTCTGG ATGGCAGAAC TCAAGAGTCG 
CGATTACGCA GTGCCTGCCG GGAGTACACT TCCCAGTACC GTGAGCTGCA GGAGAAGTAC 
CATGAGGCAA TTTACAGTGC AGCCGAGAAG GTGCTGAAGA CATCGCAAAC TGGGCAAACA 
AAGCAGTTAA AAGCATCTCT GGACAAGGTC ACTGGAGAGG TTATGCACCA GCTGCAGGAG 
GCCCGCCGCA ACGAGGTTAA GAATCTGGCC ACCGTGCACC GGGATCGTGA TGAGCTGATC 
AGAATGAAAC GCGAGGTGGC AAGCTCCGTT GTGGAACGTG GCGTAGCTGA ACGTGTGCGG 
CTGAAGCAGA CCTTTGACCG GAGAACGGAT GAACTTCAGA AGCAGCACGA CTCAGTGCGC 
AATGCCCTGG CAGAGCACCG CTCCAAGGCT CGTCAAATTC TCGACAAGGA GGCAGAATCG 
CGAAGCTGTG TATCCAGCAA TGGCTTCTTG GTCCTCTTCC ATGGACCTCA TCACCATGGC 
TGTACGGGCT CCGGCTCGTC AGCCCTTTCA GGCAATAATC TCACTCTAAA TCTGGACGCG 
GGGGCTGCCG GTAGTCATTC AGCCATTTCG CCGGCAAAGT CGCACAACAG CATCGCAGCA 
GCGGCCGAGA TGAAGACGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995604.2
CDS	692..4612
Translation	MMSAGGTYISTASVEVPQALQDGEKFIRWDDDSGTGTPVTMRVDAKGFFLYWVDQNNELDILDIATIRDVRTGQYAKRPKDNKLRQIVTLGPQDTLEEKTVTVCHGSDFVNMTFVNFCCTRRDIAQLWTDGLIKLAYSLAQLNGSAIMFLQKAHTKLCLQVDKSGRIPVKNIIKLFAQNKEDRKRVEKALDVTGLPSGKVDSISVSKFQFEDFYNLYKYLTQRSEVERLFDSIVGNSKRKCMSIAQLVEFLNKTQRDPRLNEILYPYANPARAKELIQQYEPNKFNAQKGQLSLDGFLRYLMGDDNPIMAPSKLDLCDDMDQPMSHYFINSSHNTYLTGHQLTGKSSVEIYRQCLLAGCRCVELDFWNGRTEEPVIVHGYTFVPEIFAKDVLEAIAESAFKTSEYPVILSFENHCNPRQQAKIANYCREIFGDMLLDKPLDSHPLEPNMDLPPPAMLRRKIIIKNKKKHHHHHHHHHHKKPAQVGTPAANNKLTTANSVDAKAAQQVGLSASHEDGGVTRSTANGDVATGTGTGSAAGTAGHAPPLQQIRQSSKDSTGSSDSDSSSEDESLPNTTPNLPSGNEPPPEKAQKETEAGAEISALVNYVQPIHFSSFENAEKKNRCYEMSSFDEKQATTLLKERPIEFVNYNKHQLSRVYPAGTRFDSSNFMPQLFWNAGCQLVALNFQTLDLAMQLNLGIFEYNARSGYLLKPEFMRRSDRRLDPFAESTVDGIIAGTVSITVLSGQFLTDKRANTFVEVDMYGLPADTVRKKFRTKTVRDNGMNPLYDEEPFVFKKVVLPELASIRIAAYEEGGKLIGHRVLPVIGLCPGYRHVNLRSEVGQPIALASLFLCVVVKDYVPDDLSNFAEALANPIKYQSELEKRDIQLSVLTDEAEALGSADEDLSKSLGGQKKELRPVESLATSPKHRPSISAAAAMSVDVTVDRTDGGRGEDSISIVAPSIQHQHSLDQSVSTSIRQVESSQFDVDLVLAEPLEKILDHKSVKEKRLEMEKKLESLRKKHDKEKIKIAGQKSSPLEGKKPKFAITNKLVKRLSNKSLEPGVEIPACPLDLGDSSEESAAADAGEDLAGGSSSLDGRTQESRLRSACREYTSQYRELQEKYHEAIYSAAEKVLKTSQTGQTKQLKASLDKVTGEVMHQLQEARRNEVKNLATVHRDRDELIRMKREVASSVVERGVAERVRLKQTFDRRTDELQKQHDSVRNALAEHRSKARQILDKEAESRSCVSSNGFLVLFHGPHHHGCTGSGSSALSGNNLTLNLDAGAAGSHSAISPAKSHNSIAAAAEMKT

Target ORF information:

RefSeq Version	NM_205882.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phospholipase C at 21C, transcript variant H (Plc21C), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205882.2

ORF Insert Sequence:

ATGATGAGCG CCGGAGGAAC CTACATCTCC ACCGCCAGCG TGGAGGTGCC ACAAGCTCTG 
CAAGATGGCG AGAAGTTCAT TCGATGGGAT GATGACTCGG GCACTGGAAC ACCGGTTACA 
ATGCGCGTCG ATGCCAAGGG CTTCTTCCTC TACTGGGTCG ACCAGAACAA CGAGCTGGAT 
ATCCTTGATA TAGCCACCAT ACGCGACGTT CGCACAGGAC AGTACGCTAA GCGACCAAAG 
GACAACAAGC TGCGCCAGAT TGTGACGCTG GGCCCCCAGG ACACGCTCGA GGAGAAGACA 
GTGACGGTAT GCCACGGCTC GGACTTCGTC AACATGACGT TCGTCAACTT CTGCTGCACC 
CGGCGGGACA TAGCCCAGCT CTGGACCGAC GGACTCATCA AGTTGGCCTA CAGCTTGGCC 
CAGCTCAATG GCTCGGCGAT TATGTTCCTG CAGAAGGCCC ATACCAAACT GTGTCTCCAG 
GTGGACAAAA GCGGCCGCAT ACCAGTGAAA AACATCATAA AACTTTTTGC CCAAAACAAG 
GAGGATCGCA AACGTGTCGA GAAGGCGCTG GACGTGACCG GTTTGCCGTC GGGAAAGGTC 
GATAGCATAT CGGTGTCCAA ATTTCAATTC GAGGACTTTT ACAATTTGTA CAAATATCTC 
ACGCAGCGTT CGGAGGTGGA ACGCCTCTTT GACAGCATCG TTGGCAACTC GAAGCGCAAG 
TGCATGTCCA TCGCTCAACT GGTGGAGTTC CTGAACAAGA CACAGCGTGA TCCCCGCCTG 
AACGAGATCC TCTACCCTTA TGCGAATCCC GCCCGCGCCA AGGAGCTCAT CCAGCAATAT 
GAGCCCAACA AGTTCAATGC GCAAAAGGGC CAACTCAGCC TGGATGGCTT TTTGAGGTAC 
TTAATGGGCG ACGACAATCC CATCATGGCG CCCAGCAAGC TCGATCTCTG CGATGACATG 
GACCAGCCGA TGTCGCACTA CTTCATCAAC TCCTCGCATA ACACCTACTT GACGGGACAC 
CAGCTGACCG GCAAGTCGTC CGTCGAGATT TACAGGCAGT GTCTCCTGGC GGGATGCAGA 
TGCGTTGAGC TCGACTTCTG GAACGGACGC ACCGAGGAGC CAGTCATTGT CCACGGCTAT 
ACGTTCGTGC CCGAGATATT TGCCAAGGAC GTGCTGGAGG CCATCGCAGA GAGTGCATTT 
AAAACATCCG AATACCCTGT GATATTGAGC TTTGAGAATC ACTGCAACCC TAGGCAACAG 
GCGAAAATTG CCAACTATTG TCGCGAAATA TTTGGCGATA TGCTGCTCGA TAAGCCTCTG 
GACTCTCATC CGCTGGAGCC GAACATGGAC TTGCCACCGC CGGCGATGCT GCGCCGCAAG 
ATTATAATCA AGAACAAGAA GAAGCATCAC CACCACCATC ACCATCATCA CCACAAAAAG 
CCAGCGCAGG TGGGCACGCC GGCAGCCAAC AACAAACTGA CTACAGCGAA TTCAGTGGAT 
GCCAAGGCGG CGCAACAAGT CGGTTTGTCG GCCTCGCATG AGGATGGGGG CGTGACCAGG 
AGCACCGCCA ACGGTGACGT TGCCACTGGA ACAGGAACCG GAAGTGCTGC TGGGACAGCA 
GGACACGCGC CGCCGTTGCA ACAAATCCGC CAAAGCTCCA AAGACAGCAC TGGATCCTCC 
GATTCGGACA GCTCTTCGGA GGATGAATCC CTGCCTAACA CCACGCCCAA TTTGCCCAGC 
GGCAACGAAC CTCCGCCAGA AAAGGCTCAA AAGGAAACGG AGGCGGGAGC AGAGATTTCA 
GCTTTGGTCA ACTATGTTCA GCCCATCCAT TTTAGCTCTT TTGAGAATGC GGAAAAAAAG 
AATCGATGCT ACGAAATGTC TTCGTTTGAT GAAAAACAGG CGACAACATT ATTGAAGGAG 
CGACCGATTG AATTTGTGAA CTACAACAAA CACCAATTAT CTCGTGTCTA TCCGGCGGGC 
ACTCGCTTTG ATAGCTCCAA CTTTATGCCG CAGTTGTTCT GGAATGCCGG ATGTCAGCTC 
GTGGCACTTA ACTTCCAAAC CCTCGATCTG GCCATGCAAC TCAACTTGGG GATCTTTGAG 
TATAACGCTC GGTCCGGTTA TCTACTTAAG CCGGAGTTTA TGCGCCGATC AGATCGCAGA 
TTGGATCCCT TCGCGGAAAG CACTGTGGAT GGCATCATAG CAGGCACAGT ATCAATCACG 
GTTCTATCTG GTCAGTTTCT CACGGATAAG AGGGCGAACA CTTTTGTGGA AGTTGATATG 
TACGGTTTGC CAGCGGACAC GGTGAGAAAG AAGTTTCGCA CCAAGACAGT GCGGGATAAT 
GGAATGAATC CCCTTTATGA CGAGGAGCCG TTCGTGTTTA AGAAGGTGGT TCTCCCCGAG 
TTGGCGAGCA TTCGAATTGC AGCATATGAA GAGGGTGGCA AACTCATTGG TCACCGCGTT 
TTGCCCGTAA TCGGTCTTTG CCCTGGTTAC CGACATGTGA ATCTCCGATC TGAAGTGGGT 
CAACCAATAG CCCTTGCCTC TCTTTTCCTG TGCGTGGTGG TCAAGGATTA TGTACCCGAT 
GATCTTTCCA ATTTCGCAGA GGCGCTAGCT AACCCTATTA AATACCAAAG TGAGCTAGAG 
AAGCGCGATA TTCAACTATC TGTTTTGACC GACGAAGCCG AAGCGCTGGG CAGTGCGGAC 
GAGGATCTCT CCAAGTCGTT AGGTGGCCAA AAAAAGGAGC TGCGTCCGGT TGAATCGCTT 
GCGACTTCGC CGAAGCATAG GCCGAGCATC TCGGCCGCCG CAGCAATGAG TGTTGACGTT 
ACCGTGGATC GGACCGATGG CGGAAGGGGA GAAGATAGCA TCTCTATTGT AGCACCCTCA 
ATACAGCATC AGCACTCTCT AGACCAGTCC GTAAGCACCT CCATCCGACA GGTGGAGTCC 
TCGCAGTTTG ATGTCGATCT TGTGTTGGCG GAGCCGCTGG AAAAGATATT GGATCACAAG 
TCCGTCAAGG AAAAGCGTCT CGAGATGGAA AAGAAGCTGG AGTCACTGCG AAAGAAGCAT 
GACAAGGAAA AGATTAAGAT CGCCGGCCAG AAATCCAGTC CGCTAGAGGG CAAGAAGCCG 
AAGTTTGCCA TCACGAATAA GCTGGTGAAG CGGCTAAGCA ACAAGAGTCT CGAGCCTGGT 
GTAGAGATCC CCGCCTGCCC TCTGGATCTG GGCGATAGCA GCGAGGAGAG TGCAGCCGCG 
GATGCCGGTG AGGACTTAGC CGGTGGGAGC AGCAGTCTGG ATGGCAGAAC TCAAGAGTCG 
CGATTACGCA GTGCCTGCCG GGAGTACACT TCCCAGTACC GTGAGCTGCA GGAGAAGTAC 
CATGAGGCAA TTTACAGTGC AGCCGAGAAG GTGCTGAAGA CATCGCAAAC TGGGCAAACA 
AAGCAGTTAA AAGCATCTCT GGACAAGGTC ACTGGAGAGG TTATGCACCA GCTGCAGGAG 
GCCCGCCGCA ACGAGGTTAA GAATCTGGCC ACCGTGCACC GGGATCGTGA TGAGCTGATC 
AGAATGAAAC GCGAGGTGGC AAGCTCCGTT GTGGAACGTG GCGTAGCTGA ACGTGTGCGG 
CTGAAGCAGA CCTTTGACCG GAGAACGGAT GAACTTCAGA AGCAGCACGA CTCAGTGCGC 
AATGCCCTGG CAGAGCACCG CTCCAAGGCT CGTCAAATTC TCGACAAGGA GGCAGAATCG 
CGAAGCTGTG TATCCAGCAA TGGCTTCTTG GTCCTCTTCC ATGGACCTCA TCACCATGGC 
TGTACGGGCT CCGGCTCGTC AGCCCTTTCA GGCAATAATC TCACTCTAAA TCTGGACGCG 
GGGGCTGCCG GTAGTCATTC AGCCATTTCG CCGGCAAAGT CGCACAACAG CATCGCAGCA 
GCGGCCGAGA TGAAGACGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plc21C ( NM_001272889.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259818.1 Drosophila melanogaster phospholipase C at 21C, transcript variant G (Plc21C), mRNA.

Price & Availability↑

CloneID	OFa15865
Clone ID Related Accession (Same CDS sequence)	NM_001272889.1
Accession Version	NM_001272889.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phospholipase C at 21C, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGAGCG CCGGAGGAAC CTACATCTCC ACCGCCAGCG TGGAGGTGCC ACAAGCTCTG 
CAAGATGGCG AGAAGTTCAT TCGATGGGAT GATGACTCGG GCACTGGAAC ACCGGTTACA 
ATGCGCGTCG ATGCCAAGGG CTTCTTCCTC TACTGGGTCG ACCAGAACAA CGAGCTGGAT 
ATCCTTGATA TAGCCACCAT ACGCGACGTT CGCACAGGAC AGTACGCTAA GCGACCAAAG 
GACAACAAGC TGCGCCAGAT TGTGACGCTG GGCCCCCAGG ACACGCTCGA GGAGAAGACA 
GTGACGGTAT GCCACGGCTC GGACTTCGTC AACATGACGT TCGTCAACTT CTGCTGCACC 
CGGCGGGACA TAGCCCAGCT CTGGACCGAC GGACTCATCA AGTTGGCCTA CAGCTTGGCC 
CAGCTCAATG GCTCGGCGAT TATGTTCCTG CAGAAGGCCC ATACCAAACT GTGTCTCCAG 
GTGGACAAAA GCGGCCGCAT ACCAGTGAAA AACATCATAA AACTTTTTGC CCAAAACAAG 
GAGGATCGCA AACGTGTCGA GAAGGCGCTG GACGTGACCG GTTTGCCGTC GGGAAAGGTC 
GATAGCATAT CGGTGTCCAA ATTTCAATTC GAGGACTTTT ACAATTTGTA CAAATATCTC 
ACGCAGCGTT CGGAGGTGGA ACGCCTCTTT GACAGCATCG TTGGCAACTC GAAGCGCAAG 
TGCATGTCCA TCGCTCAACT GGTGGAGTTC CTGAACAAGA CACAGCGTGA TCCCCGCCTG 
AACGAGATCC TCTACCCTTA TGCGAATCCC GCCCGCGCCA AGGAGCTCAT CCAGCAATAT 
GAGCCCAACA AGTTCAATGC GCAAAAGGGC CAACTCAGCC TGGATGGCTT TTTGAGATGC 
GTTGAGCTCG ACTTCTGGAA CGGACGCACC GAGGAGCCAG TCATTGTCCA CGGCTATACG 
TTCGTGCCCG AGATATTTGC CAAGGACGTG CTGGAGGCCA TCGCAGAGAG TGCATTTAAA 
ACATCCGAAT ACCCTGTGAT ATTGAGCTTT GAGAATCACT GCAACCCTAG GCAACAGGCG 
AAAATTGCCA ACTATTGTCG CGAAATATTT GGCGATATGC TGCTCGATAA GCCTCTGGAC 
TCTCATCCGC TGGAGCCGAA CATGGACTTG CCACCGCCGG CGATGCTGCG CCGCAAGATT 
ATAATCAAGA ACAAGAAGAA GCATCACCAC CACCATCACC ATCATCACCA CAAAAAGCCA 
GCGCAGGTGG GCACGCCGGC AGCCAACAAC AAACTGACTA CAGCGAATTC AGTGGATGCC 
AAGGCGGCGC AACAAGTCGG TTTGTCGGCC TCGCATGAGG ATGGGGGCGT GACCAGGAGC 
ACCGCCAACG GTGACGTTGC CACTGGAACA GGAACCGGAA GTGCTGCTGG GACAGCAGGA 
CACGCGCCGC CGTTGCAACA AATCCGCCAA AGCTCCAAAG ACAGCACTGG ATCCTCCGAT 
TCGGACAGCT CTTCGGAGGA TGAATCCCTG CCTAACACCA CGCCCAATTT GCCCAGCGGC 
AACGAACCTC CGCCAGAAAA GGCTCAAAAG GAAACGGAGG CGGGAGCAGA GATTTCAGCT 
TTGGTCAACT ATGTTCAGCC CATCCATTTT AGCTCTTTTG AGAATGCGGA AAAAAAGAAT 
CGATGCTACG AAATGTCTTC GTTTGATGAA AAACAGGCGA CAACATTATT GAAGGAGCGA 
CCGATTGAAT TTGTGAACTA CAACAAACAC CAATTATCTC GTGTCTATCC GGCGGGCACT 
CGCTTTGATA GCTCCAACTT TATGCCGCAG TTGTTCTGGA ATGCCGGATG TCAGCTCGTG 
GCACTTAACT TCCAAACCCT CGATCTGGCC ATGCAACTCA ACTTGGGGAT CTTTGAGTAT 
AACGCTCGGT CCGGTTATCT ACTTAAGCCG GAGTTTATGC GCCGATCAGA TCGCAGATTG 
GATCCCTTCG CGGAAAGCAC TGTGGATGGC ATCATAGCAG GCACAGTATC AATCACGGTT 
CTATCTGGTC AGTTTCTCAC GGATAAGAGG GCGAACACTT TTGTGGAAGT TGATATGTAC 
GGTTTGCCAG CGGACACGGT GAGAAAGAAG TTTCGCACCA AGACAGTGCG GGATAATGGA 
ATGAATCCCC TTTATGACGA GGAGCCGTTC GTGTTTAAGA AGGTGGTTCT CCCCGAGTTG 
GCGAGCATTC GAATTGCAGC ATATGAAGAG GGTGGCAAAC TCATTGGTCA CCGCGTTTTG 
CCCGTAATCG GTCTTTGCCC TGGTTACCGA CATGTGAATC TCCGATCTGA AGTGGGTCAA 
CCAATAGCCC TTGCCTCTCT TTTCCTGTGC GTGGTGGTCA AGGATTATGT ACCCGATGAT 
CTTTCCAATT TCGCAGAGGC GCTAGCTAAC CCTATTAAAT ACCAAAGTGA GCTAGAGAAG 
CGCGATATTC AACTATCTGT TTTGACCGAC GAAGCCGAAG CGCTGGGCAG TGCGGACGAG 
GATCTCTCCA AGTCGTTCGT TTTCGTCCAA GTAGGTGGCC AAAAAAAGGA GCTGCGTCCG 
GTTGAATCGC TTGCGACTTC GCCGAAGCAT AGGCCGAGCA TCTCGGCCGC CGCAGCAATG 
AGTGTTGACG TTACCGTGGA TCGGACCGAT GGCGGAAGGG GAGAAGATAG CATCTCTATT 
GTAGCACCCT CAATACAGCA TCAGCACTCT CTAGACCAGT CCGTAAGCAC CTCCATCCGA 
CAGGTGGAGT CCTCGCAGTT TGATGTCGAT CTTGTGTTGG CGGAGCCGCT GGAAAAGATA 
TTGGATCACA AGTCCGTCAA GGAAAAGCGT CTCGAGATGG AAAAGAAGCT GGAGTCACTG 
CGAAAGAAGC ATGACAAGGA AAAGATTAAG ATCGCCGGCC AGAAATCCAG TCCGCTAGAG 
GGCAAGAAGC CGAAGTTTGC CATCACGAAT AAGCTGGTGA AGCGGCTAAG CAACAAGAGT 
CTCGAGCCTG GTGTAGAGAT CCCCGCCTGC CCTCTGGATC TGGGCGATAG CAGCGAGGAG 
AGTGCAGCCG CGGATGCCGG TGAGGACTTA GCCGGTGGGA GCAGCAGTCT GGATGGCAGA 
ACTCAAGAGT CGCGATTACG CAGTGCCTGC CGGGAGTACA CTTCCCAGTA CCGTGAGCTG 
CAGGAGAAGT ACCATGAGGC AATTTACAGT GCAGCCGAGA AGGTGCTGAA GACATCGCAA 
ACTGGGCAAA CAAAGCAGTT AAAAGCATCT CTGGACAAGG TCACTGGAGA GGTTATGCAC 
CAGCTGCAGG AGGCCCGCCG CAACGAGGTT AAGAATCTGG CCACCGTGCA CCGGGATCGT 
GATGAGCTGA TCAGAATGAA ACGCGAGGTG GCAAGCTCCG TTGTGGAACG TGGCGTAGCT 
GAACGTGTGC GGCTGAAGCA GACCTTTGAC CGGAGAACGG ATGAACTTCA GAAGCAGCAC 
GACTCAGTGC GCAATGCCCT GGCAGAGCAC CGCTCCAAGG CTCGTCAAAT TCTCGACAAG 
GAGGCAGAAT CGCGAAGCTG TGTATCCAGC AATGGCTTCT TGGTCCTCTT CCATGGACCT 
CATCACCATG GCTGTACGGG CTCCGGCTCG TCAGCCCTTT CAGGCAATAA TCTCACTCTA 
AATCTGGACG CGGGGGCTGC CGGTAGTCAT TCAGCCATTT CGCCGGCAAA GTCGCACAAC 
AGCATCGCAG CAGCGGCCGA GATGAAGACG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259818.1
CDS	741..4493
Translation	MMSAGGTYISTASVEVPQALQDGEKFIRWDDDSGTGTPVTMRVDAKGFFLYWVDQNNELDILDIATIRDVRTGQYAKRPKDNKLRQIVTLGPQDTLEEKTVTVCHGSDFVNMTFVNFCCTRRDIAQLWTDGLIKLAYSLAQLNGSAIMFLQKAHTKLCLQVDKSGRIPVKNIIKLFAQNKEDRKRVEKALDVTGLPSGKVDSISVSKFQFEDFYNLYKYLTQRSEVERLFDSIVGNSKRKCMSIAQLVEFLNKTQRDPRLNEILYPYANPARAKELIQQYEPNKFNAQKGQLSLDGFLRCVELDFWNGRTEEPVIVHGYTFVPEIFAKDVLEAIAESAFKTSEYPVILSFENHCNPRQQAKIANYCREIFGDMLLDKPLDSHPLEPNMDLPPPAMLRRKIIIKNKKKHHHHHHHHHHKKPAQVGTPAANNKLTTANSVDAKAAQQVGLSASHEDGGVTRSTANGDVATGTGTGSAAGTAGHAPPLQQIRQSSKDSTGSSDSDSSSEDESLPNTTPNLPSGNEPPPEKAQKETEAGAEISALVNYVQPIHFSSFENAEKKNRCYEMSSFDEKQATTLLKERPIEFVNYNKHQLSRVYPAGTRFDSSNFMPQLFWNAGCQLVALNFQTLDLAMQLNLGIFEYNARSGYLLKPEFMRRSDRRLDPFAESTVDGIIAGTVSITVLSGQFLTDKRANTFVEVDMYGLPADTVRKKFRTKTVRDNGMNPLYDEEPFVFKKVVLPELASIRIAAYEEGGKLIGHRVLPVIGLCPGYRHVNLRSEVGQPIALASLFLCVVVKDYVPDDLSNFAEALANPIKYQSELEKRDIQLSVLTDEAEALGSADEDLSKSFVFVQVGGQKKELRPVESLATSPKHRPSISAAAAMSVDVTVDRTDGGRGEDSISIVAPSIQHQHSLDQSVSTSIRQVESSQFDVDLVLAEPLEKILDHKSVKEKRLEMEKKLESLRKKHDKEKIKIAGQKSSPLEGKKPKFAITNKLVKRLSNKSLEPGVEIPACPLDLGDSSEESAAADAGEDLAGGSSSLDGRTQESRLRSACREYTSQYRELQEKYHEAIYSAAEKVLKTSQTGQTKQLKASLDKVTGEVMHQLQEARRNEVKNLATVHRDRDELIRMKREVASSVVERGVAERVRLKQTFDRRTDELQKQHDSVRNALAEHRSKARQILDKEAESRSCVSSNGFLVLFHGPHHHGCTGSGSSALSGNNLTLNLDAGAAGSHSAISPAKSHNSIAAAAEMKT

Target ORF information:

RefSeq Version	NM_001272889.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phospholipase C at 21C, transcript variant G (Plc21C), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272889.1

ORF Insert Sequence:

ATGATGAGCG CCGGAGGAAC CTACATCTCC ACCGCCAGCG TGGAGGTGCC ACAAGCTCTG 
CAAGATGGCG AGAAGTTCAT TCGATGGGAT GATGACTCGG GCACTGGAAC ACCGGTTACA 
ATGCGCGTCG ATGCCAAGGG CTTCTTCCTC TACTGGGTCG ACCAGAACAA CGAGCTGGAT 
ATCCTTGATA TAGCCACCAT ACGCGACGTT CGCACAGGAC AGTACGCTAA GCGACCAAAG 
GACAACAAGC TGCGCCAGAT TGTGACGCTG GGCCCCCAGG ACACGCTCGA GGAGAAGACA 
GTGACGGTAT GCCACGGCTC GGACTTCGTC AACATGACGT TCGTCAACTT CTGCTGCACC 
CGGCGGGACA TAGCCCAGCT CTGGACCGAC GGACTCATCA AGTTGGCCTA CAGCTTGGCC 
CAGCTCAATG GCTCGGCGAT TATGTTCCTG CAGAAGGCCC ATACCAAACT GTGTCTCCAG 
GTGGACAAAA GCGGCCGCAT ACCAGTGAAA AACATCATAA AACTTTTTGC CCAAAACAAG 
GAGGATCGCA AACGTGTCGA GAAGGCGCTG GACGTGACCG GTTTGCCGTC GGGAAAGGTC 
GATAGCATAT CGGTGTCCAA ATTTCAATTC GAGGACTTTT ACAATTTGTA CAAATATCTC 
ACGCAGCGTT CGGAGGTGGA ACGCCTCTTT GACAGCATCG TTGGCAACTC GAAGCGCAAG 
TGCATGTCCA TCGCTCAACT GGTGGAGTTC CTGAACAAGA CACAGCGTGA TCCCCGCCTG 
AACGAGATCC TCTACCCTTA TGCGAATCCC GCCCGCGCCA AGGAGCTCAT CCAGCAATAT 
GAGCCCAACA AGTTCAATGC GCAAAAGGGC CAACTCAGCC TGGATGGCTT TTTGAGATGC 
GTTGAGCTCG ACTTCTGGAA CGGACGCACC GAGGAGCCAG TCATTGTCCA CGGCTATACG 
TTCGTGCCCG AGATATTTGC CAAGGACGTG CTGGAGGCCA TCGCAGAGAG TGCATTTAAA 
ACATCCGAAT ACCCTGTGAT ATTGAGCTTT GAGAATCACT GCAACCCTAG GCAACAGGCG 
AAAATTGCCA ACTATTGTCG CGAAATATTT GGCGATATGC TGCTCGATAA GCCTCTGGAC 
TCTCATCCGC TGGAGCCGAA CATGGACTTG CCACCGCCGG CGATGCTGCG CCGCAAGATT 
ATAATCAAGA ACAAGAAGAA GCATCACCAC CACCATCACC ATCATCACCA CAAAAAGCCA 
GCGCAGGTGG GCACGCCGGC AGCCAACAAC AAACTGACTA CAGCGAATTC AGTGGATGCC 
AAGGCGGCGC AACAAGTCGG TTTGTCGGCC TCGCATGAGG ATGGGGGCGT GACCAGGAGC 
ACCGCCAACG GTGACGTTGC CACTGGAACA GGAACCGGAA GTGCTGCTGG GACAGCAGGA 
CACGCGCCGC CGTTGCAACA AATCCGCCAA AGCTCCAAAG ACAGCACTGG ATCCTCCGAT 
TCGGACAGCT CTTCGGAGGA TGAATCCCTG CCTAACACCA CGCCCAATTT GCCCAGCGGC 
AACGAACCTC CGCCAGAAAA GGCTCAAAAG GAAACGGAGG CGGGAGCAGA GATTTCAGCT 
TTGGTCAACT ATGTTCAGCC CATCCATTTT AGCTCTTTTG AGAATGCGGA AAAAAAGAAT 
CGATGCTACG AAATGTCTTC GTTTGATGAA AAACAGGCGA CAACATTATT GAAGGAGCGA 
CCGATTGAAT TTGTGAACTA CAACAAACAC CAATTATCTC GTGTCTATCC GGCGGGCACT 
CGCTTTGATA GCTCCAACTT TATGCCGCAG TTGTTCTGGA ATGCCGGATG TCAGCTCGTG 
GCACTTAACT TCCAAACCCT CGATCTGGCC ATGCAACTCA ACTTGGGGAT CTTTGAGTAT 
AACGCTCGGT CCGGTTATCT ACTTAAGCCG GAGTTTATGC GCCGATCAGA TCGCAGATTG 
GATCCCTTCG CGGAAAGCAC TGTGGATGGC ATCATAGCAG GCACAGTATC AATCACGGTT 
CTATCTGGTC AGTTTCTCAC GGATAAGAGG GCGAACACTT TTGTGGAAGT TGATATGTAC 
GGTTTGCCAG CGGACACGGT GAGAAAGAAG TTTCGCACCA AGACAGTGCG GGATAATGGA 
ATGAATCCCC TTTATGACGA GGAGCCGTTC GTGTTTAAGA AGGTGGTTCT CCCCGAGTTG 
GCGAGCATTC GAATTGCAGC ATATGAAGAG GGTGGCAAAC TCATTGGTCA CCGCGTTTTG 
CCCGTAATCG GTCTTTGCCC TGGTTACCGA CATGTGAATC TCCGATCTGA AGTGGGTCAA 
CCAATAGCCC TTGCCTCTCT TTTCCTGTGC GTGGTGGTCA AGGATTATGT ACCCGATGAT 
CTTTCCAATT TCGCAGAGGC GCTAGCTAAC CCTATTAAAT ACCAAAGTGA GCTAGAGAAG 
CGCGATATTC AACTATCTGT TTTGACCGAC GAAGCCGAAG CGCTGGGCAG TGCGGACGAG 
GATCTCTCCA AGTCGTTCGT TTTCGTCCAA GTAGGTGGCC AAAAAAAGGA GCTGCGTCCG 
GTTGAATCGC TTGCGACTTC GCCGAAGCAT AGGCCGAGCA TCTCGGCCGC CGCAGCAATG 
AGTGTTGACG TTACCGTGGA TCGGACCGAT GGCGGAAGGG GAGAAGATAG CATCTCTATT 
GTAGCACCCT CAATACAGCA TCAGCACTCT CTAGACCAGT CCGTAAGCAC CTCCATCCGA 
CAGGTGGAGT CCTCGCAGTT TGATGTCGAT CTTGTGTTGG CGGAGCCGCT GGAAAAGATA 
TTGGATCACA AGTCCGTCAA GGAAAAGCGT CTCGAGATGG AAAAGAAGCT GGAGTCACTG 
CGAAAGAAGC ATGACAAGGA AAAGATTAAG ATCGCCGGCC AGAAATCCAG TCCGCTAGAG 
GGCAAGAAGC CGAAGTTTGC CATCACGAAT AAGCTGGTGA AGCGGCTAAG CAACAAGAGT 
CTCGAGCCTG GTGTAGAGAT CCCCGCCTGC CCTCTGGATC TGGGCGATAG CAGCGAGGAG 
AGTGCAGCCG CGGATGCCGG TGAGGACTTA GCCGGTGGGA GCAGCAGTCT GGATGGCAGA 
ACTCAAGAGT CGCGATTACG CAGTGCCTGC CGGGAGTACA CTTCCCAGTA CCGTGAGCTG 
CAGGAGAAGT ACCATGAGGC AATTTACAGT GCAGCCGAGA AGGTGCTGAA GACATCGCAA 
ACTGGGCAAA CAAAGCAGTT AAAAGCATCT CTGGACAAGG TCACTGGAGA GGTTATGCAC 
CAGCTGCAGG AGGCCCGCCG CAACGAGGTT AAGAATCTGG CCACCGTGCA CCGGGATCGT 
GATGAGCTGA TCAGAATGAA ACGCGAGGTG GCAAGCTCCG TTGTGGAACG TGGCGTAGCT 
GAACGTGTGC GGCTGAAGCA GACCTTTGAC CGGAGAACGG ATGAACTTCA GAAGCAGCAC 
GACTCAGTGC GCAATGCCCT GGCAGAGCAC CGCTCCAAGG CTCGTCAAAT TCTCGACAAG 
GAGGCAGAAT CGCGAAGCTG TGTATCCAGC AATGGCTTCT TGGTCCTCTT CCATGGACCT 
CATCACCATG GCTGTACGGG CTCCGGCTCG TCAGCCCTTT CAGGCAATAA TCTCACTCTA 
AATCTGGACG CGGGGGCTGC CGGTAGTCAT TCAGCCATTT CGCCGGCAAA GTCGCACAAC 
AGCATCGCAG CAGCGGCCGA GATGAAGACG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Plc21C cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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