Pi3K21B cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pi3K21B
Entrez Gene ID	33203
Full Name	CG2699 gene product from transcript CG2699-RE
Synonyms	CG2699,Dmel\CG2699,Dp60,PI(3)K,PI3K,PI3K-p60,PI3K21B,PI[[3]]K,Pi3K21B,p60,Pi3Kp60,Pi3k21B,dP60,dPI3K,droPIK57,p60
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 21B8-21B8

mRNA and Protein(s)

mRNA	Protein	Name
NM_001272887.1	NP_001259816.1	Pi3K21B, isoform D
NM_001272888.1	NP_001259817.1	Pi3K21B, isoform E
NM_057922.4	NP_477270.2	Pi3K21B, isoform B
NM_001272886.1	NP_001259815.1	Pi3K21B, isoform C

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Pi3K21B	NP_001259817.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005583	XP_001688684.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

dS6K resides on an insulin signalling pathway distinct from that of dPKB, and surprisingly also from that of dPI(3)K.
Title: dS6K-regulated cell growth is dPKB/dPI(3)K-independent, but requires dPDK1.

The following Pi3K21B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pi3K21B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa13330	NM_057922.4 Latest version!	Drosophila melanogaster Pi3K21B, transcript variant B (Pi3K21B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa13330	NM_001272888.1 Latest version!	Drosophila melanogaster Pi3K21B, transcript variant E (Pi3K21B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa15864	NM_001272886.1 Latest version!	Drosophila melanogaster Pi3K21B, transcript variant C (Pi3K21B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa15864	NM_001272887.1 Latest version!	Drosophila melanogaster Pi3K21B, transcript variant D (Pi3K21B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pi3K21B ( NM_001272888.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259817.1 Drosophila melanogaster Pi3K21B, transcript variant E (Pi3K21B), mRNA.

Price & Availability↑

CloneID	OFa13330
Clone ID Related Accession (Same CDS sequence)	NM_001272888.1 , NM_057922.4
Accession Version	NM_001272888.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Pi3K21B, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGGCCAC AGGCGCCCGG ATCGCTGGTC GATCCCAACG AGGACGAGCT ACGCATGGCC 
CCATGGTACT GGGGCCGGAT ATCGAGGGAG GAAGCCAAGA GCATCCTGCA CGGAAAGCCG 
GACGGCAGCT TTTTGGTGCG GGACGCCCTC TCGATGAAGG GCGAGTACAC TCTGACCCTG 
ATGAAGGACG GATGCGAGAA GCTAATAAAG ATATGCCACA TGGATCGCAA GTACGGCTTT 
ATCGAGACGG ACCTGTTCAA TTCGGTGGTG GAGATGATCA ACTACTACAA GGAGAACTCT 
CTGAGCATGT ACAACAAGAC GCTGGACATA ACACTGAGTA ATCCGATTGT GCGAGCCCGC 
GAGGATGAGG AGTCGCAGCC GCACGGGGAT CTTTGTCTGC TGAGCAACGA ATTCATCAGG 
ACCTGCCAGC TGCTTCAGAA TCTTGAGCAA AATCTCGAGA ATAAGCGCAA CTCATTCAAT 
GCGATTCGAG AGGAGCTGCA GGAGAAGAAG CTGCACCAGA GCGTATTCGG GAATACAGAG 
AAGATATTCC GCAATCAAAT CAAGCTCAAT GAATCCTTCA TGAAGGCGCC AGCGGATGCC 
CCGTCCACGG AGGCAGGAGG AGCTGGAGAT GGAGCTAACG CAGCCGCATC CGCAGCGGCC 
AATGCCAACG CTCGCCGGAG TCTGCAGGAG CACAAGCAGA CACTCCTCAA TCTGCTGGAT 
GCTCTTCAGG CCAAGGGTCA AGTCCTTAAC CACTACATGG AGAACAAGAA GAAGGAGGAG 
CTACTGCTCG AGCGACAGAT CAACGCCCTG AAGCCGGAAT TACAGATACT GCAGTTGCGC 
AAGGACAAGT ACATTGAGCG CCTAAAAGGA TTCAACCTCA AGGACGACGA TCTGAAGATG 
ATATTGCAGA TGGGCTTCGA CAAGTGGCAG CAACTTTATG AGACAGTCTC CAACCAGCCG 
CACAGCAACG AAGCTCTCTG GTTGCTAAAG GATGCCAAGA GAAGGAATGC GGAGGAGATG 
CTTAAGGGGG CGCCATCGGG CACCTTCCTG ATTCGAGCGA GAGATGCGGG TCACTATGCT 
CTGTCCATTG CCTGCAAGAA CATCGTGCAG CACTGCCTTA TTTACGAGAC GAGCACCGGC 
TTTGGATTTG CTGCCCCTTA CAATATATAT GCCACGCTGA AGAGTCTGGT GGAGCACTAT 
GCCAACAATT CGCTGGAGGA GCATAACGAC ACGCTGACCA CGACATTGCG CTGGCCTGTC 
TTGTACTGGA AGAACAACCC GCTGCAAGTT CAGATGATCC AATTGCAGGA GGAAATGGAT 
CTGGAGTACG AGCAGGCAGC CACGTTGAGG CCGCCGCCAA TGATGGGCAG CAGTGCGCCG 
ATTCCCACGA GTCGGTCCCG TGAACATGAC GTCGTAGATG GAACTGGAAG CCTCGAGGCG 
GAGGCGGCTC CGGCCTCCAT TTCGCCCTCC AACTTCAGCA CATCGCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259817.1
CDS	554..2044
Translation	MRPQAPGSLVDPNEDELRMAPWYWGRISREEAKSILHGKPDGSFLVRDALSMKGEYTLTLMKDGCEKLIKICHMDRKYGFIETDLFNSVVEMINYYKENSLSMYNKTLDITLSNPIVRAREDEESQPHGDLCLLSNEFIRTCQLLQNLEQNLENKRNSFNAIREELQEKKLHQSVFGNTEKIFRNQIKLNESFMKAPADAPSTEAGGAGDGANAAASAAANANARRSLQEHKQTLLNLLDALQAKGQVLNHYMENKKKEELLLERQINALKPELQILQLRKDKYIERLKGFNLKDDDLKMILQMGFDKWQQLYETVSNQPHSNEALWLLKDAKRRNAEEMLKGAPSGTFLIRARDAGHYALSIACKNIVQHCLIYETSTGFGFAAPYNIYATLKSLVEHYANNSLEEHNDTLTTTLRWPVLYWKNNPLQVQMIQLQEEMDLEYEQAATLRPPPMMGSSAPIPTSRSREHDVVDGTGSLEAEAAPASISPSNFSTSQ

Target ORF information:

RefSeq Version	NM_001272888.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Pi3K21B, transcript variant E (Pi3K21B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272888.1

ORF Insert Sequence:

ATGCGGCCAC AGGCGCCCGG ATCGCTGGTC GATCCCAACG AGGACGAGCT ACGCATGGCC 
CCATGGTACT GGGGCCGGAT ATCGAGGGAG GAAGCCAAGA GCATCCTGCA CGGAAAGCCG 
GACGGCAGCT TTTTGGTGCG GGACGCCCTC TCGATGAAGG GCGAGTACAC TCTGACCCTG 
ATGAAGGACG GATGCGAGAA GCTAATAAAG ATATGCCACA TGGATCGCAA GTACGGCTTT 
ATCGAGACGG ACCTGTTCAA TTCGGTGGTG GAGATGATCA ACTACTACAA GGAGAACTCT 
CTGAGCATGT ACAACAAGAC GCTGGACATA ACACTGAGTA ATCCGATTGT GCGAGCCCGC 
GAGGATGAGG AGTCGCAGCC GCACGGGGAT CTTTGTCTGC TGAGCAACGA ATTCATCAGG 
ACCTGCCAGC TGCTTCAGAA TCTTGAGCAA AATCTCGAGA ATAAGCGCAA CTCATTCAAT 
GCGATTCGAG AGGAGCTGCA GGAGAAGAAG CTGCACCAGA GCGTATTCGG GAATACAGAG 
AAGATATTCC GCAATCAAAT CAAGCTCAAT GAATCCTTCA TGAAGGCGCC AGCGGATGCC 
CCGTCCACGG AGGCAGGAGG AGCTGGAGAT GGAGCTAACG CAGCCGCATC CGCAGCGGCC 
AATGCCAACG CTCGCCGGAG TCTGCAGGAG CACAAGCAGA CACTCCTCAA TCTGCTGGAT 
GCTCTTCAGG CCAAGGGTCA AGTCCTTAAC CACTACATGG AGAACAAGAA GAAGGAGGAG 
CTACTGCTCG AGCGACAGAT CAACGCCCTG AAGCCGGAAT TACAGATACT GCAGTTGCGC 
AAGGACAAGT ACATTGAGCG CCTAAAAGGA TTCAACCTCA AGGACGACGA TCTGAAGATG 
ATATTGCAGA TGGGCTTCGA CAAGTGGCAG CAACTTTATG AGACAGTCTC CAACCAGCCG 
CACAGCAACG AAGCTCTCTG GTTGCTAAAG GATGCCAAGA GAAGGAATGC GGAGGAGATG 
CTTAAGGGGG CGCCATCGGG CACCTTCCTG ATTCGAGCGA GAGATGCGGG TCACTATGCT 
CTGTCCATTG CCTGCAAGAA CATCGTGCAG CACTGCCTTA TTTACGAGAC GAGCACCGGC 
TTTGGATTTG CTGCCCCTTA CAATATATAT GCCACGCTGA AGAGTCTGGT GGAGCACTAT 
GCCAACAATT CGCTGGAGGA GCATAACGAC ACGCTGACCA CGACATTGCG CTGGCCTGTC 
TTGTACTGGA AGAACAACCC GCTGCAAGTT CAGATGATCC AATTGCAGGA GGAAATGGAT 
CTGGAGTACG AGCAGGCAGC CACGTTGAGG CCGCCGCCAA TGATGGGCAG CAGTGCGCCG 
ATTCCCACGA GTCGGTCCCG TGAACATGAC GTCGTAGATG GAACTGGAAG CCTCGAGGCG 
GAGGCGGCTC CGGCCTCCAT TTCGCCCTCC AACTTCAGCA CATCGCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pi3K21B ( NM_057922.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477270.2 Drosophila melanogaster Pi3K21B, transcript variant B (Pi3K21B), mRNA.

Price & Availability↑

CloneID	OFa13330
Clone ID Related Accession (Same CDS sequence)	NM_001272888.1 , NM_057922.4
Accession Version	NM_057922.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Pi3K21B, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_057922.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGGCCAC AGGCGCCCGG ATCGCTGGTC GATCCCAACG AGGACGAGCT ACGCATGGCC 
CCATGGTACT GGGGCCGGAT ATCGAGGGAG GAAGCCAAGA GCATCCTGCA CGGAAAGCCG 
GACGGCAGCT TTTTGGTGCG GGACGCCCTC TCGATGAAGG GCGAGTACAC TCTGACCCTG 
ATGAAGGACG GATGCGAGAA GCTAATAAAG ATATGCCACA TGGATCGCAA GTACGGCTTT 
ATCGAGACGG ACCTGTTCAA TTCGGTGGTG GAGATGATCA ACTACTACAA GGAGAACTCT 
CTGAGCATGT ACAACAAGAC GCTGGACATA ACACTGAGTA ATCCGATTGT GCGAGCCCGC 
GAGGATGAGG AGTCGCAGCC GCACGGGGAT CTTTGTCTGC TGAGCAACGA ATTCATCAGG 
ACCTGCCAGC TGCTTCAGAA TCTTGAGCAA AATCTCGAGA ATAAGCGCAA CTCATTCAAT 
GCGATTCGAG AGGAGCTGCA GGAGAAGAAG CTGCACCAGA GCGTATTCGG GAATACAGAG 
AAGATATTCC GCAATCAAAT CAAGCTCAAT GAATCCTTCA TGAAGGCGCC AGCGGATGCC 
CCGTCCACGG AGGCAGGAGG AGCTGGAGAT GGAGCTAACG CAGCCGCATC CGCAGCGGCC 
AATGCCAACG CTCGCCGGAG TCTGCAGGAG CACAAGCAGA CACTCCTCAA TCTGCTGGAT 
GCTCTTCAGG CCAAGGGTCA AGTCCTTAAC CACTACATGG AGAACAAGAA GAAGGAGGAG 
CTACTGCTCG AGCGACAGAT CAACGCCCTG AAGCCGGAAT TACAGATACT GCAGTTGCGC 
AAGGACAAGT ACATTGAGCG CCTAAAAGGA TTCAACCTCA AGGACGACGA TCTGAAGATG 
ATATTGCAGA TGGGCTTCGA CAAGTGGCAG CAACTTTATG AGACAGTCTC CAACCAGCCG 
CACAGCAACG AAGCTCTCTG GTTGCTAAAG GATGCCAAGA GAAGGAATGC GGAGGAGATG 
CTTAAGGGGG CGCCATCGGG CACCTTCCTG ATTCGAGCGA GAGATGCGGG TCACTATGCT 
CTGTCCATTG CCTGCAAGAA CATCGTGCAG CACTGCCTTA TTTACGAGAC GAGCACCGGC 
TTTGGATTTG CTGCCCCTTA CAATATATAT GCCACGCTGA AGAGTCTGGT GGAGCACTAT 
GCCAACAATT CGCTGGAGGA GCATAACGAC ACGCTGACCA CGACATTGCG CTGGCCTGTC 
TTGTACTGGA AGAACAACCC GCTGCAAGTT CAGATGATCC AATTGCAGGA GGAAATGGAT 
CTGGAGTACG AGCAGGCAGC CACGTTGAGG CCGCCGCCAA TGATGGGCAG CAGTGCGCCG 
ATTCCCACGA GTCGGTCCCG TGAACATGAC GTCGTAGATG GAACTGGAAG CCTCGAGGCG 
GAGGCGGCTC CGGCCTCCAT TTCGCCCTCC AACTTCAGCA CATCGCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477270.2
CDS	554..2044
Translation	MRPQAPGSLVDPNEDELRMAPWYWGRISREEAKSILHGKPDGSFLVRDALSMKGEYTLTLMKDGCEKLIKICHMDRKYGFIETDLFNSVVEMINYYKENSLSMYNKTLDITLSNPIVRAREDEESQPHGDLCLLSNEFIRTCQLLQNLEQNLENKRNSFNAIREELQEKKLHQSVFGNTEKIFRNQIKLNESFMKAPADAPSTEAGGAGDGANAAASAAANANARRSLQEHKQTLLNLLDALQAKGQVLNHYMENKKKEELLLERQINALKPELQILQLRKDKYIERLKGFNLKDDDLKMILQMGFDKWQQLYETVSNQPHSNEALWLLKDAKRRNAEEMLKGAPSGTFLIRARDAGHYALSIACKNIVQHCLIYETSTGFGFAAPYNIYATLKSLVEHYANNSLEEHNDTLTTTLRWPVLYWKNNPLQVQMIQLQEEMDLEYEQAATLRPPPMMGSSAPIPTSRSREHDVVDGTGSLEAEAAPASISPSNFSTSQ

Target ORF information:

RefSeq Version	NM_057922.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Pi3K21B, transcript variant B (Pi3K21B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057922.4

ORF Insert Sequence:

ATGCGGCCAC AGGCGCCCGG ATCGCTGGTC GATCCCAACG AGGACGAGCT ACGCATGGCC 
CCATGGTACT GGGGCCGGAT ATCGAGGGAG GAAGCCAAGA GCATCCTGCA CGGAAAGCCG 
GACGGCAGCT TTTTGGTGCG GGACGCCCTC TCGATGAAGG GCGAGTACAC TCTGACCCTG 
ATGAAGGACG GATGCGAGAA GCTAATAAAG ATATGCCACA TGGATCGCAA GTACGGCTTT 
ATCGAGACGG ACCTGTTCAA TTCGGTGGTG GAGATGATCA ACTACTACAA GGAGAACTCT 
CTGAGCATGT ACAACAAGAC GCTGGACATA ACACTGAGTA ATCCGATTGT GCGAGCCCGC 
GAGGATGAGG AGTCGCAGCC GCACGGGGAT CTTTGTCTGC TGAGCAACGA ATTCATCAGG 
ACCTGCCAGC TGCTTCAGAA TCTTGAGCAA AATCTCGAGA ATAAGCGCAA CTCATTCAAT 
GCGATTCGAG AGGAGCTGCA GGAGAAGAAG CTGCACCAGA GCGTATTCGG GAATACAGAG 
AAGATATTCC GCAATCAAAT CAAGCTCAAT GAATCCTTCA TGAAGGCGCC AGCGGATGCC 
CCGTCCACGG AGGCAGGAGG AGCTGGAGAT GGAGCTAACG CAGCCGCATC CGCAGCGGCC 
AATGCCAACG CTCGCCGGAG TCTGCAGGAG CACAAGCAGA CACTCCTCAA TCTGCTGGAT 
GCTCTTCAGG CCAAGGGTCA AGTCCTTAAC CACTACATGG AGAACAAGAA GAAGGAGGAG 
CTACTGCTCG AGCGACAGAT CAACGCCCTG AAGCCGGAAT TACAGATACT GCAGTTGCGC 
AAGGACAAGT ACATTGAGCG CCTAAAAGGA TTCAACCTCA AGGACGACGA TCTGAAGATG 
ATATTGCAGA TGGGCTTCGA CAAGTGGCAG CAACTTTATG AGACAGTCTC CAACCAGCCG 
CACAGCAACG AAGCTCTCTG GTTGCTAAAG GATGCCAAGA GAAGGAATGC GGAGGAGATG 
CTTAAGGGGG CGCCATCGGG CACCTTCCTG ATTCGAGCGA GAGATGCGGG TCACTATGCT 
CTGTCCATTG CCTGCAAGAA CATCGTGCAG CACTGCCTTA TTTACGAGAC GAGCACCGGC 
TTTGGATTTG CTGCCCCTTA CAATATATAT GCCACGCTGA AGAGTCTGGT GGAGCACTAT 
GCCAACAATT CGCTGGAGGA GCATAACGAC ACGCTGACCA CGACATTGCG CTGGCCTGTC 
TTGTACTGGA AGAACAACCC GCTGCAAGTT CAGATGATCC AATTGCAGGA GGAAATGGAT 
CTGGAGTACG AGCAGGCAGC CACGTTGAGG CCGCCGCCAA TGATGGGCAG CAGTGCGCCG 
ATTCCCACGA GTCGGTCCCG TGAACATGAC GTCGTAGATG GAACTGGAAG CCTCGAGGCG 
GAGGCGGCTC CGGCCTCCAT TTCGCCCTCC AACTTCAGCA CATCGCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pi3K21B ( NM_001272887.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259816.1 Drosophila melanogaster Pi3K21B, transcript variant D (Pi3K21B), mRNA.

Price & Availability↑

CloneID	OFa15864
Clone ID Related Accession (Same CDS sequence)	NM_001272887.1 , NM_001272886.1
Accession Version	NM_001272887.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Pi3K21B, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATATTGC AGATGGGCTT CGACAAGTGG CAGCAACTTT ATGAGACAGT CTCCAACCAG 
CCGCACAGCA ACGAAGCTCT CTGGTTGCTA AAGGATGCCA AGAGAAGGAA TGCGGAGGAG 
ATGCTTAAGG GGGCGCCATC GGGCACCTTC CTGATTCGAG CGAGAGATGC GGGTCACTAT 
GCTCTGTCCA TTGCCTGCAA GAACATCGTG CAGCACTGCC TTATTTACGA GACGAGCACC 
GGCTTTGGAT TTGCTGCCCC TTACAATATA TATGCCACGC TGAAGAGTCT GGTGGAGCAC 
TATGCCAACA ATTCGCTGGA GGAGCATAAC GACACGCTGA CCACGACATT GCGCTGGCCT 
GTCTTGTACT GGAAGAACAA CCCGCTGCAA GTTCAGATGA TCCAATTGCA GGAGGAAATG 
GATCTGGAGT ACGAGCAGGC AGCCACGTTG AGGCCGCCGC CAATGATGGG CAGCAGTGCG 
CCGATTCCCA CGAGTCGGTC CCGTGAACAT GACGTCGTAG ATGGAACTGG AAGCCTCGAG 
GCGGAGGCGG CTCCGGCCTC CATTTCGCCC TCCAACTTCA GCACATCGCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259816.1
CDS	280..873
Translation	MILQMGFDKWQQLYETVSNQPHSNEALWLLKDAKRRNAEEMLKGAPSGTFLIRARDAGHYALSIACKNIVQHCLIYETSTGFGFAAPYNIYATLKSLVEHYANNSLEEHNDTLTTTLRWPVLYWKNNPLQVQMIQLQEEMDLEYEQAATLRPPPMMGSSAPIPTSRSREHDVVDGTGSLEAEAAPASISPSNFSTSQ

Target ORF information:

RefSeq Version	NM_001272887.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Pi3K21B, transcript variant D (Pi3K21B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272887.1

ORF Insert Sequence:

ATGATATTGC AGATGGGCTT CGACAAGTGG CAGCAACTTT ATGAGACAGT CTCCAACCAG 
CCGCACAGCA ACGAAGCTCT CTGGTTGCTA AAGGATGCCA AGAGAAGGAA TGCGGAGGAG 
ATGCTTAAGG GGGCGCCATC GGGCACCTTC CTGATTCGAG CGAGAGATGC GGGTCACTAT 
GCTCTGTCCA TTGCCTGCAA GAACATCGTG CAGCACTGCC TTATTTACGA GACGAGCACC 
GGCTTTGGAT TTGCTGCCCC TTACAATATA TATGCCACGC TGAAGAGTCT GGTGGAGCAC 
TATGCCAACA ATTCGCTGGA GGAGCATAAC GACACGCTGA CCACGACATT GCGCTGGCCT 
GTCTTGTACT GGAAGAACAA CCCGCTGCAA GTTCAGATGA TCCAATTGCA GGAGGAAATG 
GATCTGGAGT ACGAGCAGGC AGCCACGTTG AGGCCGCCGC CAATGATGGG CAGCAGTGCG 
CCGATTCCCA CGAGTCGGTC CCGTGAACAT GACGTCGTAG ATGGAACTGG AAGCCTCGAG 
GCGGAGGCGG CTCCGGCCTC CATTTCGCCC TCCAACTTCA GCACATCGCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pi3K21B ( NM_001272886.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259815.1 Drosophila melanogaster Pi3K21B, transcript variant C (Pi3K21B), mRNA.

Price & Availability↑

CloneID	OFa15864
Clone ID Related Accession (Same CDS sequence)	NM_001272887.1 , NM_001272886.1
Accession Version	NM_001272886.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Pi3K21B, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATATTGC AGATGGGCTT CGACAAGTGG CAGCAACTTT ATGAGACAGT CTCCAACCAG 
CCGCACAGCA ACGAAGCTCT CTGGTTGCTA AAGGATGCCA AGAGAAGGAA TGCGGAGGAG 
ATGCTTAAGG GGGCGCCATC GGGCACCTTC CTGATTCGAG CGAGAGATGC GGGTCACTAT 
GCTCTGTCCA TTGCCTGCAA GAACATCGTG CAGCACTGCC TTATTTACGA GACGAGCACC 
GGCTTTGGAT TTGCTGCCCC TTACAATATA TATGCCACGC TGAAGAGTCT GGTGGAGCAC 
TATGCCAACA ATTCGCTGGA GGAGCATAAC GACACGCTGA CCACGACATT GCGCTGGCCT 
GTCTTGTACT GGAAGAACAA CCCGCTGCAA GTTCAGATGA TCCAATTGCA GGAGGAAATG 
GATCTGGAGT ACGAGCAGGC AGCCACGTTG AGGCCGCCGC CAATGATGGG CAGCAGTGCG 
CCGATTCCCA CGAGTCGGTC CCGTGAACAT GACGTCGTAG ATGGAACTGG AAGCCTCGAG 
GCGGAGGCGG CTCCGGCCTC CATTTCGCCC TCCAACTTCA GCACATCGCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259815.1
CDS	361..954
Translation	MILQMGFDKWQQLYETVSNQPHSNEALWLLKDAKRRNAEEMLKGAPSGTFLIRARDAGHYALSIACKNIVQHCLIYETSTGFGFAAPYNIYATLKSLVEHYANNSLEEHNDTLTTTLRWPVLYWKNNPLQVQMIQLQEEMDLEYEQAATLRPPPMMGSSAPIPTSRSREHDVVDGTGSLEAEAAPASISPSNFSTSQ

Target ORF information:

RefSeq Version	NM_001272886.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Pi3K21B, transcript variant C (Pi3K21B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272886.1

ORF Insert Sequence:

ATGATATTGC AGATGGGCTT CGACAAGTGG CAGCAACTTT ATGAGACAGT CTCCAACCAG 
CCGCACAGCA ACGAAGCTCT CTGGTTGCTA AAGGATGCCA AGAGAAGGAA TGCGGAGGAG 
ATGCTTAAGG GGGCGCCATC GGGCACCTTC CTGATTCGAG CGAGAGATGC GGGTCACTAT 
GCTCTGTCCA TTGCCTGCAA GAACATCGTG CAGCACTGCC TTATTTACGA GACGAGCACC 
GGCTTTGGAT TTGCTGCCCC TTACAATATA TATGCCACGC TGAAGAGTCT GGTGGAGCAC 
TATGCCAACA ATTCGCTGGA GGAGCATAAC GACACGCTGA CCACGACATT GCGCTGGCCT 
GTCTTGTACT GGAAGAACAA CCCGCTGCAA GTTCAGATGA TCCAATTGCA GGAGGAAATG 
GATCTGGAGT ACGAGCAGGC AGCCACGTTG AGGCCGCCGC CAATGATGGG CAGCAGTGCG 
CCGATTCCCA CGAGTCGGTC CCGTGAACAT GACGTCGTAG ATGGAACTGG AAGCCTCGAG 
GCGGAGGCGG CTCCGGCCTC CATTTCGCCC TCCAACTTCA GCACATCGCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Pi3K21B cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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