Zir cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Zir
Entrez Gene ID	33165
Full Name	Zizimin-related
Synonyms	CG11376,Dm zir,Dmel\CG11376,FBgn 31216,anon-EST:Posey54
General protein information	Preferred Names Zizimin-related Names CG11376 gene product from transcript CG11376-RA CG11376-PA Zir-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 21B1-21B2

mRNA and Protein(s)

mRNA	Protein	Name
NM_134645.3	NP_608489.2	Zizimin-related

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	DOCK7	XP_005620391.1
Bos taurus (cattle)	DOCK7	NP_001179835.2
Danio rerio (zebrafish)	dock7	XP_005165943.1
Caenorhabditis elegans (roundworm)	F46H5.4	NP_509219.1
Mus musculus (house mouse)	Dock7	NP_080358.3
Rattus norvegicus (Norway rat)	Dock7	NP_001178503.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010192	XP_319370.4
Arabidopsis thaliana (thale cress)	SPK1	NP_193367.7
Homo sapiens (human)	DOCK7	NP_212132.2
	DOCK7	XP_001085641.2
Gallus gallus (chicken)	DOCK7	XP_004936918.1
Drosophila melanogaster (fruit fly)	Zir	NP_608489.2
Xenopus tropicalis (tropical clawed frog)	dock6	XP_004913993.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Zir is necessary to activate the Rho-family GTPases Rac2 and Cdc42 during the Drosophila cellular immune response.
Title: The RhoGEF Zizimin-related acts in the Drosophila cellular immune response via the Rho GTPases Rac2 and Cdc42.

The following Zir gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Zir cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa15840	NM_134645.3 Latest version!	Drosophila melanogaster Zizimin-related (Zir), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Zir ( NM_134645.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_608489.2 Drosophila melanogaster Zizimin-related (Zir), mRNA.

Price & Availability↑

CloneID	OFa15840
Clone ID Related Accession (Same CDS sequence)	NM_134645.3
Accession Version	NM_134645.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6195bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Zizimin-related
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_134645.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGAGTG CCCAGCGCTC GTTTGTGCAA AAAGTTTCCA AGCAGTCGGC TGCGGATGTA 
CGAAAAAATG TTTCTGGTTG CCATCTATCA AAGGCAATGG ACCCATCATT GTGTGGTTCT 
TCGATCTCCC CGACAGAGCC CCTTGATTAC GAAGAATTTC TATCCCAGCA TATGAACATC 
ATCAACCGGG ATCCCTTGAA ACATATATTG GACTTTCCTC AAGGGGATGT AACTGTTAAA 
ATCATTCCCC GAAAAATCAG AACTGTTGAT CACGTTGTTC CCAATGAGAA TTTGTCTGAT 
TTGCCCCATC ACGTACAAGA ATGTGTTAAC TGCTATACGC GCCCATGGAA GGTGGTTGAA 
TATGCACAGC GGCACCTTTC CAGTTCTTGC TATATCCGCG AGCGTATCGA CCGCGGAACC 
ATAAGTCCCT CTGCGTACCA GCAGGAATTC GAGATCGACA AGGATTTTTC GTCATTTGAA 
GAGGCGTTCG CTTATAAAAG CGAGAGCTGC ACTCCAAGTT CTCGACAATC CATTGCAAGT 
TTGGCTTCAG TAAGCTCTTG TACGGACACT TTGACCCCGC GTGGTTCTTG GGCTAGCTTT 
GATCTTCGAC GTTCGGTTAA TGACCCACTA ATACCCAATT TGCTTGACAA TGTACCTCCG 
GAGCATATTG ATCAATCCAA TGAAGCACGC CGGCAGCAAG ATCGACAAAT GGCTCTTTTC 
TCACTGTATC CGGAATCTGA AGCGGAGGAA AACATTGAAC GCCGCCTGCT CGCAGAGATT 
CCAGTTGAAC ATATGGGTCA TCGCATTCAG GTTAACTGCC TACAGCTTCG GCTTGAGCTG 
GAAGTGGAGC CAATTTTTGC ATCGATGGCT ATTTATGATG CTAAGGAACG GCAAAAAATT 
TCTGAAAATT TCTATTTTGA CATGAACGCA GATAGTCTTA AACGCATGTT GTCAAGTCAT 
GTACAGTGCG CCGACATAAG TACCCAGAGC CACTCAGCCA TATTCGAAAT TTCATATCCG 
AGTAACGATT TATTTCTCGT GATTCGTCTG GAAAAAGTTT TGCAGGGTGA CATAAACAAT 
TCCGTCGAGC CGTATCTCAA AGAAGACAAA GATAAATACC GAGAGAAAGT AAAATCCAAC 
GCTGTGGACT ACTGTGAACG ACTAGGCAAA TACCGAATGC CGTTTGCATG GACAGGAATC 
TACCTAACAA ATGTTTTTAA TGGCGATAAC TTTGAAAGCA AAGACGCTGG CGCCGCGGAA 
CGGGATTCCT TTGGAAATGC TTGTGGCAGC AGCTTGGGCA CAGCGCCAAG CTCCAATAGC 
TTAGATCGAA AATCTTCCAC AAGTAGCTTT GATCAACTAA GGCGAAAAGC TAACGATATG 
AGCGGAACAC TTACACGACG TGGTTCGCTT GAACGGAAGG AAAAGCACCG ATCCTGGTCC 
CCAGATGATT TTGCAAACGT TGTAGAAAAC TTTCGTCCTA TCACTATAAC AGTACCAAGC 
TTCTTTAAAC AAGAGGCAGA TAAAATGAAA GACGAGGATT TGTACAAAAT TTTACCAGAG 
TTAAAACGAC CGAGCTCAGT TATGAAGAAG TACAAATGCA TACCTGGTTC TATTAAATTA 
GAAATATCAC CTTGCGTCGA GGAAGCCAAG AATGCATTAA CGCCAGAGTT AGCACCTATA 
AACCCTCAAA GTGCAGATAA TGTGCGCCCA GTTAAGGAAA TCTTAGAATT TCCGCAATCG 
GCGATTTACA ATCCACATTA TAGCTATCGC AATCTCCTAT TTGTGTCACC AAAGGAGCTA 
AATTTTTCTT CGCGTGCAGG TTCTGCTCGA AACATTGCCG TTCGCGTTCA ACTAATGGCT 
GGAGAAACGC CAAAAGATGC GGTCAATGCC ATCTACGGCA AGTCGTCTTG TCCCAAATTT 
TCTACTGAAG CATTTACAGC TGTCAATTAT CATAACAAAT GCCCATCATT CTACGATGAA 
ATAAAAATTG CGCTGCCTGC ATCAATAAAG CAGCATCACC ATTTATTGTT CACCATTTAC 
CATGTTTCAT GTCAAAAGAA ACCACAGGAC TTGCAGCCGT CAGTAGAGAC TCCTATCGGC 
TACACATGGT TGCCCTTACT GGAAGATGGA AAACTTAAGT TTGGGGAATT TAATCTTCCT 
GTTATGGTAG AATCACCGCC GGAAAATTAC TCATTTATAC CACCGAATGT TCACCTGCCT 
GGAATTAAAT GGCTGGACAA TCACAGAGCC GTGTTTTCTA TTAATGTAGA AGCAGTCACC 
GCAATTCATA CTTTGGATTC TTTCCTCGAT CGATTCTTCT TAATTTGCGA ATATCTAGAC 
ACTCGAAATA TACCTTCCCA TATTGGCGAG AATAATATAG AAACAGAATT AAAGAAATGC 
TTGCTGGATA TCGAATATGC AAATCGTGAG CCATTAGTGA GGCATCTACC ATTAGTCTTG 
GATAAGCTTA TCGAATTGTT AGTTGTCACC CACAAAGTTG GTGGACAAGC AATGTCTCTG 
GGATCTACGG TTTTCGAAGT TCTTTGTTTG GTATCATCTT TGTTATCAAT ACTTAACGAC 
GATCAATATG ACCAATACGG TCGGCAAAGT TTGCTATCAA CATATGTGCA CTTTCAATGT 
AAAATTCCGC ACCCCTTTCA AAGCAAACAG CGACTAACAT GCAGTCGCAG CACAACAGAA 
GACTTGCAAT TAAGCGAATC GTACACCATA TATGATAATG TTTTAGCAAG TGGCCGAAGC 
TTGGATCGCA AAGAGCTGTC CATAGATGTC CTTCACTCGA TGGCTGCACG GGATGTCCAA 
GTGCGGCTGC TTCACGAAGA ATTAGCCTTG CATTGGGTTG TTGCAAGTGG AAAGGCAGCT 
GACTTAGCCA TGTCAAATTC GTGGTTTTTA TTTGAGCTTA TTGTCAAATC TATGATTGAA 
CATTTGCATT GTTCAAATAC TTTAAATGGT CCCCGCAAAC ATCGATTTCC ACATCAGTTT 
AATGATGATC TATCCACCCT GGTGCACTTA GTTACAACAA AAGTGGTCGG CTACCACAGC 
AACGAACCTA AATTAGCGCA GTCATTAAAT GCAAGTTTAG GCTTTTTTAT ATTTGACATA 
CTAAGCATCA TGGATCGGGG ATTTGTTTTC GGCCTCATCA AAACGTACAC AAAGGTTCTG 
ATTTCTAAAA ATGCTTCAAT ACCGGATCTA ATGAACTATA AAATAGACTT TTTGAGAATT 
GTGTGTAGTC ATGAGCATTT TGTAGCCTTA AACTTGCCGT TTGGTACTTC ATACACAATG 
GTAACAGCGC CATGCAGCCC CACGCCAAGC ACAACGTCGA GCAACAGCCA AACTTCTTGC 
GGATCACTAG AGAGAGCCCT TCACGCCGAC CTTAGTCAAG AATTCCTCCA GCAACATTTT 
CTAGTCGGAC TTGTTCTGAG CGACTTAGCA GCAGTAATGG AGGTGCCAAA CCCCCAATTA 
CATGGAAAAG CAATAAGGTG TATTCGCAAC CTGATGACAT CTCACGATTT GGACGCGCGG 
TATAGCGAAA CTGATGCACG TGCAAGGGTG GCTGCTTTAT ATATACCGCT ACTTTCTATA 
GTGATGGATT GCATTCCTCA GTTGCATCAG CACGGCCTTG AGCAGGATCG TCTGCAGCAA 
ATCGGTCAAC TTGAAGACTA TCAAGGGCCA CATCAAACTA TTACAGCATC AACCATAAAT 
CCGGAAGTGG CATTTGCGAT ATCGGGAAGT CGTCCCTACT CCTACTTGAA TGAACAAGTT 
AAAAACAAAT TGCCCCTCAG CTCGGAGAAT ACCCGCCATT TATTGGTGTG TTTTCTGTGG 
GTGCTAAAAA ACTTGGAGCG CAACGTTTTA TATCGCTGGC TTATGGATTT AAGTCCGCAT 
AGAGTGCACC AAATGCTTCA AGGTGTTAAC GTTTGCTTGA AAACTTTCGA GTACACAGGA 
CAAAAAAACG TGGCCACCCT TAAACGAACG AATACACAAA GTTTTAGGAA AACTGGATCT 
ACTGATGTTA AGGAAAAGCT GGAAGAATGT ATTAGAGGCA CAAATTCAGC ACGTTATGAT 
TTAATAAATC GTCGAAAAGA TCGTAATTCA ACAGAAAAAT TCCGATGGAG GAAAGACCAG 
ATGCCATATC GCTCTCAATA TGCGGATGCT GTGGGAAAAA GTGAGCATGA ACTTGAATTA 
AGTCATTTCA TCGAAGGCTC TTTGGCAACT GAAGTTGCAC TTGTACTTCT CGATACCCTT 
GAAATAATTG TTCATGCTGC TGCCAACCTT TATCACAATC TTCTTGGAAC TGTGCTAAAG 
GTGCTTCTTC ATTCTTTGTC CCGGAATCAA TCTGTTTTGG CCCTGCAAAA CTTGTTTGCC 
TCCCAACGCG CGTTAATCTT TAAATTTCCA AACTTATTAT TTGATGACGA GACCGATATA 
TGTGCGGATT TGTGTCTAAT TTTACTGAAA CATTGTGGGT CCCTATTGCC AGGAATACGA 
TCACAGGCAG CTGCATCACT ATATTTACTA ATGAGACAAA ATTTTGAAAT TGGAAATAAC 
TTTGCCCGAG TTAAGATGCA AGTGACGATG TCTTTAAGCT CCCTAGTTGG AACAAGTTCG 
GTCTTTAGTG AGCAATCCTT ACGCCGTGCG CTTAAAACCG TCCTGGTTTA TGCTGAATCC 
GACTCCGACC TGCAGGATAC TTCTTTTCCT GAACAAGTAC AAGATTTGCT TTTTAATCTG 
CATATGATCC TGTCAGATAC TGTTAAAATG AAGGAGTATC AGGAAGACCC AGAAATGTTG 
CTTGACCTCA TGAATCGTAT TGCCAAGGGA TACCAAAATA ACCCTGATCT ACGACTGACT 
TGGTTGGAAA ATATGGCTAA AAAACACCGC GAGCGAGCAA ATCACACGGA AGCAGCCATG 
TGTTATGTAC ATGCTGCTTC TTTAGTTTCT GAATATCTTA GCATGTTGGA GTCACAAAAA 
CATTTGCCTG TTGGAGCTGT AAGTTTTCAA CGAATTTCTC CCAACACACT AATGGAGTCG 
GCCGTATCGG ATGATGTGCT AAGTCCCGGC GAAGATGGTA TCTGCCTAGG AAATCATTTC 
ACTGAAACTG GGTTGAAGGC CTTGCTGGAA GAAGCCTCCA ATTCTTTTCA AGTTGCTGGC 
ATGTATGAAG CAATGAACGA AGTGTACAAA ATTCTAATAC CCATATGCGA GGCTAACAGA 
GATTTTCAAA AGCTAAGCAA AGTTCATGGC AAATTGCAGG AGGCATTTAA TCGAATATCC 
CAACTACAGG GTAAGAGAGT TTTTGGAACA TACTTTCGTG TTGGCTTCTA TGGCGGAAAA 
TTTGGGGACT TGGATCAGCA GGAATTCATT TATAAAGAGC CAACATTGAC GAAGTTGCCC 
GAAATATTTA GTCGGCTTCA GAACTTTTAC ACTGAACGAT TCGGACCGGA CTCTGTGCAT 
ATCATTAAAG ATTCCAATAC CGTTGATATT AATAGCTTGG ATCCCGATAA GGCTTACATT 
CAAATTACTT ATGTTGAACC CTACTTTGAA ACATATGAAA TGCGTCATCG TGAGACATAC 
TTTGAGCGGA ATTTCAATAT AAAACGTTTT ATATATGCCA CGCCTTTTAC TAAAAATGGA 
AAGGCACATG GCGAACTAAA TGAGCAGTGT AAACGGAAAA CGATTTTGAC CACGGCGAAT 
CACTTTCCAT ACGTAAAAAC TCGCATTATG GTAATTAGCC GACAGCAAAT AGTCCTGGAA 
CCGATTGAAG TGGCAATTGA AGATATTCAA AAAAAAACAT TGGAATTGGC AGCTGCCACG 
AATCAAGAGC CAGCAGACCC CAAAATATTG CAAATGGTAC TGCAGGGATG TATTGGAACC 
ACTGTTAACC AAGGACCGAT GGAAATGGCG AGTGTGTTCC TTTCCAATTT ATCCGACGGA 
ACAACTGTAC CGACAAAACA CCAAAACAAA CTTCGGTTGT GCTTTCGCGA GTTTTCAAAG 
CGTTGTGCTG ATGCTTTGAA AAAGAATCGC AATCTAATAC TTTCAGATCA AAAAGATTAC 
CAACGAGAAC TGGAACGTAA CAACGATCGG TTCATTGAAC GATTGACTCC CTTTATTACT 
CTTACATCAG TTCAGAATCA TGGCGTTGTC AAGGCCAACG CGTACAACAA CAAAAGTACT 
CCTTTGAAAT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_608489.2
CDS	114..6308
Translation	MSSAQRSFVQKVSKQSAADVRKNVSGCHLSKAMDPSLCGSSISPTEPLDYEEFLSQHMNIINRDPLKHILDFPQGDVTVKIIPRKIRTVDHVVPNENLSDLPHHVQECVNCYTRPWKVVEYAQRHLSSSCYIRERIDRGTISPSAYQQEFEIDKDFSSFEEAFAYKSESCTPSSRQSIASLASVSSCTDTLTPRGSWASFDLRRSVNDPLIPNLLDNVPPEHIDQSNEARRQQDRQMALFSLYPESEAEENIERRLLAEIPVEHMGHRIQVNCLQLRLELEVEPIFASMAIYDAKERQKISENFYFDMNADSLKRMLSSHVQCADISTQSHSAIFEISYPSNDLFLVIRLEKVLQGDINNSVEPYLKEDKDKYREKVKSNAVDYCERLGKYRMPFAWTGIYLTNVFNGDNFESKDAGAAERDSFGNACGSSLGTAPSSNSLDRKSSTSSFDQLRRKANDMSGTLTRRGSLERKEKHRSWSPDDFANVVENFRPITITVPSFFKQEADKMKDEDLYKILPELKRPSSVMKKYKCIPGSIKLEISPCVEEAKNALTPELAPINPQSADNVRPVKEILEFPQSAIYNPHYSYRNLLFVSPKELNFSSRAGSARNIAVRVQLMAGETPKDAVNAIYGKSSCPKFSTEAFTAVNYHNKCPSFYDEIKIALPASIKQHHHLLFTIYHVSCQKKPQDLQPSVETPIGYTWLPLLEDGKLKFGEFNLPVMVESPPENYSFIPPNVHLPGIKWLDNHRAVFSINVEAVTAIHTLDSFLDRFFLICEYLDTRNIPSHIGENNIETELKKCLLDIEYANREPLVRHLPLVLDKLIELLVVTHKVGGQAMSLGSTVFEVLCLVSSLLSILNDDQYDQYGRQSLLSTYVHFQCKIPHPFQSKQRLTCSRSTTEDLQLSESYTIYDNVLASGRSLDRKELSIDVLHSMAARDVQVRLLHEELALHWVVASGKAADLAMSNSWFLFELIVKSMIEHLHCSNTLNGPRKHRFPHQFNDDLSTLVHLVTTKVVGYHSNEPKLAQSLNASLGFFIFDILSIMDRGFVFGLIKTYTKVLISKNASIPDLMNYKIDFLRIVCSHEHFVALNLPFGTSYTMVTAPCSPTPSTTSSNSQTSCGSLERALHADLSQEFLQQHFLVGLVLSDLAAVMEVPNPQLHGKAIRCIRNLMTSHDLDARYSETDARARVAALYIPLLSIVMDCIPQLHQHGLEQDRLQQIGQLEDYQGPHQTITASTINPEVAFAISGSRPYSYLNEQVKNKLPLSSENTRHLLVCFLWVLKNLERNVLYRWLMDLSPHRVHQMLQGVNVCLKTFEYTGQKNVATLKRTNTQSFRKTGSTDVKEKLEECIRGTNSARYDLINRRKDRNSTEKFRWRKDQMPYRSQYADAVGKSEHELELSHFIEGSLATEVALVLLDTLEIIVHAAANLYHNLLGTVLKVLLHSLSRNQSVLALQNLFASQRALIFKFPNLLFDDETDICADLCLILLKHCGSLLPGIRSQAAASLYLLMRQNFEIGNNFARVKMQVTMSLSSLVGTSSVFSEQSLRRALKTVLVYAESDSDLQDTSFPEQVQDLLFNLHMILSDTVKMKEYQEDPEMLLDLMNRIAKGYQNNPDLRLTWLENMAKKHRERANHTEAAMCYVHAASLVSEYLSMLESQKHLPVGAVSFQRISPNTLMESAVSDDVLSPGEDGICLGNHFTETGLKALLEEASNSFQVAGMYEAMNEVYKILIPICEANRDFQKLSKVHGKLQEAFNRISQLQGKRVFGTYFRVGFYGGKFGDLDQQEFIYKEPTLTKLPEIFSRLQNFYTERFGPDSVHIIKDSNTVDINSLDPDKAYIQITYVEPYFETYEMRHRETYFERNFNIKRFIYATPFTKNGKAHGELNEQCKRKTILTTANHFPYVKTRIMVISRQQIVLEPIEVAIEDIQKKTLELAAATNQEPADPKILQMVLQGCIGTTVNQGPMEMASVFLSNLSDGTTVPTKHQNKLRLCFREFSKRCADALKKNRNLILSDQKDYQRELERNNDRFIERLTPFITLTSVQNHGVVKANAYNNKSTPLKW

Target ORF information:

RefSeq Version	NM_134645.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Zizimin-related (Zir), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134645.3

ORF Insert Sequence:

ATGTCGAGTG CCCAGCGCTC GTTTGTGCAA AAAGTTTCCA AGCAGTCGGC TGCGGATGTA 
CGAAAAAATG TTTCTGGTTG CCATCTATCA AAGGCAATGG ACCCATCATT GTGTGGTTCT 
TCGATCTCCC CGACAGAGCC CCTTGATTAC GAAGAATTTC TATCCCAGCA TATGAACATC 
ATCAACCGGG ATCCCTTGAA ACATATATTG GACTTTCCTC AAGGGGATGT AACTGTTAAA 
ATCATTCCCC GAAAAATCAG AACTGTTGAT CACGTTGTTC CCAATGAGAA TTTGTCTGAT 
TTGCCCCATC ACGTACAAGA ATGTGTTAAC TGCTATACGC GCCCATGGAA GGTGGTTGAA 
TATGCACAGC GGCACCTTTC CAGTTCTTGC TATATCCGCG AGCGTATCGA CCGCGGAACC 
ATAAGTCCCT CTGCGTACCA GCAGGAATTC GAGATCGACA AGGATTTTTC GTCATTTGAA 
GAGGCGTTCG CTTATAAAAG CGAGAGCTGC ACTCCAAGTT CTCGACAATC CATTGCAAGT 
TTGGCTTCAG TAAGCTCTTG TACGGACACT TTGACCCCGC GTGGTTCTTG GGCTAGCTTT 
GATCTTCGAC GTTCGGTTAA TGACCCACTA ATACCCAATT TGCTTGACAA TGTACCTCCG 
GAGCATATTG ATCAATCCAA TGAAGCACGC CGGCAGCAAG ATCGACAAAT GGCTCTTTTC 
TCACTGTATC CGGAATCTGA AGCGGAGGAA AACATTGAAC GCCGCCTGCT CGCAGAGATT 
CCAGTTGAAC ATATGGGTCA TCGCATTCAG GTTAACTGCC TACAGCTTCG GCTTGAGCTG 
GAAGTGGAGC CAATTTTTGC ATCGATGGCT ATTTATGATG CTAAGGAACG GCAAAAAATT 
TCTGAAAATT TCTATTTTGA CATGAACGCA GATAGTCTTA AACGCATGTT GTCAAGTCAT 
GTACAGTGCG CCGACATAAG TACCCAGAGC CACTCAGCCA TATTCGAAAT TTCATATCCG 
AGTAACGATT TATTTCTCGT GATTCGTCTG GAAAAAGTTT TGCAGGGTGA CATAAACAAT 
TCCGTCGAGC CGTATCTCAA AGAAGACAAA GATAAATACC GAGAGAAAGT AAAATCCAAC 
GCTGTGGACT ACTGTGAACG ACTAGGCAAA TACCGAATGC CGTTTGCATG GACAGGAATC 
TACCTAACAA ATGTTTTTAA TGGCGATAAC TTTGAAAGCA AAGACGCTGG CGCCGCGGAA 
CGGGATTCCT TTGGAAATGC TTGTGGCAGC AGCTTGGGCA CAGCGCCAAG CTCCAATAGC 
TTAGATCGAA AATCTTCCAC AAGTAGCTTT GATCAACTAA GGCGAAAAGC TAACGATATG 
AGCGGAACAC TTACACGACG TGGTTCGCTT GAACGGAAGG AAAAGCACCG ATCCTGGTCC 
CCAGATGATT TTGCAAACGT TGTAGAAAAC TTTCGTCCTA TCACTATAAC AGTACCAAGC 
TTCTTTAAAC AAGAGGCAGA TAAAATGAAA GACGAGGATT TGTACAAAAT TTTACCAGAG 
TTAAAACGAC CGAGCTCAGT TATGAAGAAG TACAAATGCA TACCTGGTTC TATTAAATTA 
GAAATATCAC CTTGCGTCGA GGAAGCCAAG AATGCATTAA CGCCAGAGTT AGCACCTATA 
AACCCTCAAA GTGCAGATAA TGTGCGCCCA GTTAAGGAAA TCTTAGAATT TCCGCAATCG 
GCGATTTACA ATCCACATTA TAGCTATCGC AATCTCCTAT TTGTGTCACC AAAGGAGCTA 
AATTTTTCTT CGCGTGCAGG TTCTGCTCGA AACATTGCCG TTCGCGTTCA ACTAATGGCT 
GGAGAAACGC CAAAAGATGC GGTCAATGCC ATCTACGGCA AGTCGTCTTG TCCCAAATTT 
TCTACTGAAG CATTTACAGC TGTCAATTAT CATAACAAAT GCCCATCATT CTACGATGAA 
ATAAAAATTG CGCTGCCTGC ATCAATAAAG CAGCATCACC ATTTATTGTT CACCATTTAC 
CATGTTTCAT GTCAAAAGAA ACCACAGGAC TTGCAGCCGT CAGTAGAGAC TCCTATCGGC 
TACACATGGT TGCCCTTACT GGAAGATGGA AAACTTAAGT TTGGGGAATT TAATCTTCCT 
GTTATGGTAG AATCACCGCC GGAAAATTAC TCATTTATAC CACCGAATGT TCACCTGCCT 
GGAATTAAAT GGCTGGACAA TCACAGAGCC GTGTTTTCTA TTAATGTAGA AGCAGTCACC 
GCAATTCATA CTTTGGATTC TTTCCTCGAT CGATTCTTCT TAATTTGCGA ATATCTAGAC 
ACTCGAAATA TACCTTCCCA TATTGGCGAG AATAATATAG AAACAGAATT AAAGAAATGC 
TTGCTGGATA TCGAATATGC AAATCGTGAG CCATTAGTGA GGCATCTACC ATTAGTCTTG 
GATAAGCTTA TCGAATTGTT AGTTGTCACC CACAAAGTTG GTGGACAAGC AATGTCTCTG 
GGATCTACGG TTTTCGAAGT TCTTTGTTTG GTATCATCTT TGTTATCAAT ACTTAACGAC 
GATCAATATG ACCAATACGG TCGGCAAAGT TTGCTATCAA CATATGTGCA CTTTCAATGT 
AAAATTCCGC ACCCCTTTCA AAGCAAACAG CGACTAACAT GCAGTCGCAG CACAACAGAA 
GACTTGCAAT TAAGCGAATC GTACACCATA TATGATAATG TTTTAGCAAG TGGCCGAAGC 
TTGGATCGCA AAGAGCTGTC CATAGATGTC CTTCACTCGA TGGCTGCACG GGATGTCCAA 
GTGCGGCTGC TTCACGAAGA ATTAGCCTTG CATTGGGTTG TTGCAAGTGG AAAGGCAGCT 
GACTTAGCCA TGTCAAATTC GTGGTTTTTA TTTGAGCTTA TTGTCAAATC TATGATTGAA 
CATTTGCATT GTTCAAATAC TTTAAATGGT CCCCGCAAAC ATCGATTTCC ACATCAGTTT 
AATGATGATC TATCCACCCT GGTGCACTTA GTTACAACAA AAGTGGTCGG CTACCACAGC 
AACGAACCTA AATTAGCGCA GTCATTAAAT GCAAGTTTAG GCTTTTTTAT ATTTGACATA 
CTAAGCATCA TGGATCGGGG ATTTGTTTTC GGCCTCATCA AAACGTACAC AAAGGTTCTG 
ATTTCTAAAA ATGCTTCAAT ACCGGATCTA ATGAACTATA AAATAGACTT TTTGAGAATT 
GTGTGTAGTC ATGAGCATTT TGTAGCCTTA AACTTGCCGT TTGGTACTTC ATACACAATG 
GTAACAGCGC CATGCAGCCC CACGCCAAGC ACAACGTCGA GCAACAGCCA AACTTCTTGC 
GGATCACTAG AGAGAGCCCT TCACGCCGAC CTTAGTCAAG AATTCCTCCA GCAACATTTT 
CTAGTCGGAC TTGTTCTGAG CGACTTAGCA GCAGTAATGG AGGTGCCAAA CCCCCAATTA 
CATGGAAAAG CAATAAGGTG TATTCGCAAC CTGATGACAT CTCACGATTT GGACGCGCGG 
TATAGCGAAA CTGATGCACG TGCAAGGGTG GCTGCTTTAT ATATACCGCT ACTTTCTATA 
GTGATGGATT GCATTCCTCA GTTGCATCAG CACGGCCTTG AGCAGGATCG TCTGCAGCAA 
ATCGGTCAAC TTGAAGACTA TCAAGGGCCA CATCAAACTA TTACAGCATC AACCATAAAT 
CCGGAAGTGG CATTTGCGAT ATCGGGAAGT CGTCCCTACT CCTACTTGAA TGAACAAGTT 
AAAAACAAAT TGCCCCTCAG CTCGGAGAAT ACCCGCCATT TATTGGTGTG TTTTCTGTGG 
GTGCTAAAAA ACTTGGAGCG CAACGTTTTA TATCGCTGGC TTATGGATTT AAGTCCGCAT 
AGAGTGCACC AAATGCTTCA AGGTGTTAAC GTTTGCTTGA AAACTTTCGA GTACACAGGA 
CAAAAAAACG TGGCCACCCT TAAACGAACG AATACACAAA GTTTTAGGAA AACTGGATCT 
ACTGATGTTA AGGAAAAGCT GGAAGAATGT ATTAGAGGCA CAAATTCAGC ACGTTATGAT 
TTAATAAATC GTCGAAAAGA TCGTAATTCA ACAGAAAAAT TCCGATGGAG GAAAGACCAG 
ATGCCATATC GCTCTCAATA TGCGGATGCT GTGGGAAAAA GTGAGCATGA ACTTGAATTA 
AGTCATTTCA TCGAAGGCTC TTTGGCAACT GAAGTTGCAC TTGTACTTCT CGATACCCTT 
GAAATAATTG TTCATGCTGC TGCCAACCTT TATCACAATC TTCTTGGAAC TGTGCTAAAG 
GTGCTTCTTC ATTCTTTGTC CCGGAATCAA TCTGTTTTGG CCCTGCAAAA CTTGTTTGCC 
TCCCAACGCG CGTTAATCTT TAAATTTCCA AACTTATTAT TTGATGACGA GACCGATATA 
TGTGCGGATT TGTGTCTAAT TTTACTGAAA CATTGTGGGT CCCTATTGCC AGGAATACGA 
TCACAGGCAG CTGCATCACT ATATTTACTA ATGAGACAAA ATTTTGAAAT TGGAAATAAC 
TTTGCCCGAG TTAAGATGCA AGTGACGATG TCTTTAAGCT CCCTAGTTGG AACAAGTTCG 
GTCTTTAGTG AGCAATCCTT ACGCCGTGCG CTTAAAACCG TCCTGGTTTA TGCTGAATCC 
GACTCCGACC TGCAGGATAC TTCTTTTCCT GAACAAGTAC AAGATTTGCT TTTTAATCTG 
CATATGATCC TGTCAGATAC TGTTAAAATG AAGGAGTATC AGGAAGACCC AGAAATGTTG 
CTTGACCTCA TGAATCGTAT TGCCAAGGGA TACCAAAATA ACCCTGATCT ACGACTGACT 
TGGTTGGAAA ATATGGCTAA AAAACACCGC GAGCGAGCAA ATCACACGGA AGCAGCCATG 
TGTTATGTAC ATGCTGCTTC TTTAGTTTCT GAATATCTTA GCATGTTGGA GTCACAAAAA 
CATTTGCCTG TTGGAGCTGT AAGTTTTCAA CGAATTTCTC CCAACACACT AATGGAGTCG 
GCCGTATCGG ATGATGTGCT AAGTCCCGGC GAAGATGGTA TCTGCCTAGG AAATCATTTC 
ACTGAAACTG GGTTGAAGGC CTTGCTGGAA GAAGCCTCCA ATTCTTTTCA AGTTGCTGGC 
ATGTATGAAG CAATGAACGA AGTGTACAAA ATTCTAATAC CCATATGCGA GGCTAACAGA 
GATTTTCAAA AGCTAAGCAA AGTTCATGGC AAATTGCAGG AGGCATTTAA TCGAATATCC 
CAACTACAGG GTAAGAGAGT TTTTGGAACA TACTTTCGTG TTGGCTTCTA TGGCGGAAAA 
TTTGGGGACT TGGATCAGCA GGAATTCATT TATAAAGAGC CAACATTGAC GAAGTTGCCC 
GAAATATTTA GTCGGCTTCA GAACTTTTAC ACTGAACGAT TCGGACCGGA CTCTGTGCAT 
ATCATTAAAG ATTCCAATAC CGTTGATATT AATAGCTTGG ATCCCGATAA GGCTTACATT 
CAAATTACTT ATGTTGAACC CTACTTTGAA ACATATGAAA TGCGTCATCG TGAGACATAC 
TTTGAGCGGA ATTTCAATAT AAAACGTTTT ATATATGCCA CGCCTTTTAC TAAAAATGGA 
AAGGCACATG GCGAACTAAA TGAGCAGTGT AAACGGAAAA CGATTTTGAC CACGGCGAAT 
CACTTTCCAT ACGTAAAAAC TCGCATTATG GTAATTAGCC GACAGCAAAT AGTCCTGGAA 
CCGATTGAAG TGGCAATTGA AGATATTCAA AAAAAAACAT TGGAATTGGC AGCTGCCACG 
AATCAAGAGC CAGCAGACCC CAAAATATTG CAAATGGTAC TGCAGGGATG TATTGGAACC 
ACTGTTAACC AAGGACCGAT GGAAATGGCG AGTGTGTTCC TTTCCAATTT ATCCGACGGA 
ACAACTGTAC CGACAAAACA CCAAAACAAA CTTCGGTTGT GCTTTCGCGA GTTTTCAAAG 
CGTTGTGCTG ATGCTTTGAA AAAGAATCGC AATCTAATAC TTTCAGATCA AAAAGATTAC 
CAACGAGAAC TGGAACGTAA CAACGATCGG TTCATTGAAC GATTGACTCC CTTTATTACT 
CTTACATCAG TTCAGAATCA TGGCGTTGTC AAGGCCAACG CGTACAACAA CAAAAGTACT 
CCTTTGAAAT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Zir cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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