dbr cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	dbr
Entrez Gene ID	33161
Full Name	debra
Synonyms	BcDNA:LD26519,CG11371,Dbr,Debra,Dmel\CG11371,EP0456,EP456
General protein information	Preferred Names debra Names CG11371 gene product from transcript CG11371-RF CG11371-PB CG11371-PC CG11371-PD CG11371-PE CG11371-PF dbr-PB dbr-PC dbr-PD dbr-PE dbr-PF
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 21B1-21B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001258880.2	NP_001245809.1	debra, isoform C
NM_134642.3	NP_608486.1	debra, isoform B
NM_001258882.2	NP_001245811.1	debra, isoform E
NM_001258883.3	NP_001245812.1	debra, isoform F
NM_001258881.2	NP_001245810.1	debra, isoform D

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

debra encodes a protein involved in the Hedgehog signaling pathway as a mediator of protein degradation by the lysosome.
Title: Debra, a protein mediating lysosomal degradation, is required for long-term memory in Drosophila.

The following dbr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dbr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa13297	NM_001258880.2 Latest version!	Drosophila melanogaster debra (dbr), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa13297	NM_001258881.2 Latest version!	Drosophila melanogaster debra (dbr), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa13297	NM_001258882.2 Latest version!	Drosophila melanogaster debra (dbr), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa13297	NM_001258883.3 Latest version!	Drosophila melanogaster debra (dbr), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa13297	NM_134642.3 Latest version!	Drosophila melanogaster debra (dbr), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dbr ( NM_001258880.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245809.1 Drosophila melanogaster debra (dbr), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa13297
Clone ID Related Accession (Same CDS sequence)	NM_001258880.2 , NM_134642.3 , NM_001258882.2 , NM_001258883.3 , NM_001258881.2
Accession Version	NM_001258880.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3024bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	debra, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001258880.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATGCTC AGTTTGAGCA CTTGTGCCGC ATTTGCGCTG CCAACACGAA AAGCAAGACG 
AATTCCGTGG TTGAAAGTGT GTTTATTTTT AAAACACAGG GACTGAAGGA TAAAATATCT 
CGACACCTTT ATCTAAATGT AGCTGAAGAA GATCCTCTGC CAAAAGTGCT CTGCAAATCT 
TGTTACCGCC AAGTAGAGGC CACGGCGTCG CTTTCAAATA TAGCAAAACA TACTCAGCTC 
GTGTTTCGGG ACTTTCTCCT TAGTACACTG CCTAAGAATG CGAGGGAAGC GGCTGCTGCA 
CAACTGAGCG TTCCAGCAAC CCAAATCCCA CACCGACACG ACGAAGTCCA AGCGGAAATC 
GATGAATTTC GCCCCATTTT GCTGACCCAC GTGCCGGAGT CCGGAAACAC CGCTTCCAAG 
CCCCAACCAG CCAATTGCCT GGAGCTGGCC CCAAAAACCG GCGAAAACTA CTTGGCTGCA 
TCTTCCTCAT CCTCTTCTCT GAGCAAACCC GCTGGATCAG TAAAGAGCTA TCTTAACACT 
GGGAGACGCA ACAGCGTTTT TACAGACACT AGGTATGGAT CGGCCCCACT GTCTATCGCT 
CAACGAACTA TGCAGTGTCT GCCTTCTTTG GTGCCCCTAA ACACAGAGTC ATTGGGTAGC 
AACGCAATGA GAAAGAGCCC AGACACAAAA CTATCGCCGA AAGGAGTAAT AAACTTCATC 
CAGACTCATG GGCGCATCAC GGGCAATATA CCTGAAGTCC AAGCAGAGGA CAATACCGGA 
AACATCAACG TGACAGATAG CAGAAACACA GTTACAGCTT CCGTTCTTAT TTCTTCAAAC 
GTTAGCAATC CAGGAACGAA ATCTCAGGAC AGTAATTTGT TTCTGCAGAA AAGTAGAAAT 
CTTAAAAACA CATTAAACAA CATTAAAGCC ATGCGTACCG GACAGGTATC ACTGCTTAAG 
GGTGCGCAAA ATCGAAACCC TATTGAAGAT GTTAGACCAC TGGTCACAAC TACACTGGTA 
CCCCAATATG GTGGTGAACC TTCCGTGGAG GAAGCACTGC CAGAGCACAT GATCTTTGCG 
GCGCCAAAAA AGAAAAGGGA GGATGAAGAA CAATTTCTTC AAACGGCTCT GCCACAGCTA 
AAGAAAACTG TGCCAAGTCA ATCTAAGACA AAGTCCACAA AACTCGATTT AACTATATCG 
GTTGATTCGC ATGTTTCACC CCCGTCAACG TCAATTTCTA ATGTTAAAAG CCTCACTGAT 
GCAATATTCC TGGGAAGCGT TATTAGGGAT CCAGACTTGT TGATGCTTAT TCTCAAGGCT 
CTAAAATGGC CGGTGACAGC AGAAAACTGT GATGAGCAAA TGTCCCGCTT AAAAAGTTCA 
AAGTTCGCTG TTATAATGTC AGACACAAAC CTTCTTCAAG ACACAGATCT CACACAACTT 
TTAGGTCCAT ATTTGGCCCC AATGCTGCCT GTGGTTCAGC AACAAGATCA GCATCCACTT 
TCCTCAGCTA TCGGGTCTGC TTCTTCAAAC CATTCATCGC AGGCGACTAA AGATGTACTT 
AAGCTGGCTG ACTTATCTAG TGCAATGCCT TATAAACTTC CACCAGAAAC GTCGGTTCAA 
TTAGTGCCTT CCAGTCCCAC GGAACAGGAA CCGCAGCCTG TGCGTCACAA AAAGGCGGTT 
AGCGTGCCAA CTGTCAAACG CCATCAACGA AAGATGCGTG CCCTTGATTT ACCTGCTGTT 
GACAAGACGC GCCGCATAAT TTCGTTATCG ACTTCAAAGG CAGCTTATGT CAGCAACGAA 
CTGTCGAATA TCAATTCTGT GCTTTTTTCG CAGTTCGAAT CAAATCCGGC CGACGCTATT 
AATGAAGCGC TTATCTCACT CCTTAAGCAG CAACAACAAG AAACAAAAGA CCAGCGAAAA 
ACTCGTCAGA GTTGTATTGG CTCTGACAGT GCAGTTAATT TAGAAGACAT TGTACTGATC 
GATCCCCAAC CGCCTCTTGC AGTCGATCCG CATAATGTGA AGTACCTTGA GGATGACTAC 
GTCTCTCCGC AGTCCCAGCC CGGATCCCAA TACAATTTTA AAAGGCGCAA AACAGACATT 
CGGTCATCAG AGCCGTCTGA AGTCGTTGTG CTCGATAACA GCGGATCCCC AAATGAAAAG 
AATTCAAAAG AAGTATTTCC AAGTACGAAA CAGACTTTAT CCAGAGAAAC AAAGAAAAGT 
GCGGTAACTG TTAGAAAAGA ATCTTTGGGT CAGCTAAAGC AGGCACCCAC AAGTACCGTC 
CCGGAAGACA TTTGCGATCC TAAAGCCAGA GCCATTAATG CGAAAGAAAA TCCCGAAGTG 
TGTGAACCAG TCCCGGACAA TGGAGGTAGT GACGATATTG ATGATAGCAA AGCAGGCTCG 
GTATCCGTTC GGAAGTCGGA AGTCAAAGCT GCGCTTGGAC AAAAGCTTTT GGAGGCTATT 
GGACTTTCTC AGTTGGGAGC CGATGCAGCG CCTGAAAACT CCCGCGACAC TCTTAGAAGT 
GCGTTAAAGC GCTCTATAAA ACAGGCCCAG GAGCAACAGC TTCAGCTGAA GCGAGGCAAT 
CATGAAGAAC CTGTGAAGCA ACTTGCCGAT TCCACAACCA AGCTAAGTGC AGCAGAATCG 
GCAGATGAGC ATAAAAAAGC AATTGCCGAG CGGGAGCTGG CCCTGCTGAA TCGTGGATTT 
AAAGCAGGAG AAGAAAACAC GATTTCCCTA AAAGATGATA AGTGGGAGCG AACTGATGTT 
AGTTCTTCAT CATCAAGAAA CCGCCGAGGA CGTAAATCAA AGATTGATTC CAACGTCGAG 
GATTTTGGCG ATGAAGAAAA AAAAGACTCT AGATGGGATG AAGACGATGA CTTGCCGTTA 
AAGGCAGATA CGGAAAAACC TTCAGAACGG AACAGCACCA ACGCCCGACC GACCCGTACT 
AGCAAAACGT TATCAAAGTA TTATAAAGGA CCAACTTCTA AATCACATCA TAGCATGGGT 
ACGCGCTCGA CACGACAGCG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245809.1
CDS	522..3545
Translation	MDAQFEHLCRICAANTKSKTNSVVESVFIFKTQGLKDKISRHLYLNVAEEDPLPKVLCKSCYRQVEATASLSNIAKHTQLVFRDFLLSTLPKNAREAAAAQLSVPATQIPHRHDEVQAEIDEFRPILLTHVPESGNTASKPQPANCLELAPKTGENYLAASSSSSSLSKPAGSVKSYLNTGRRNSVFTDTRYGSAPLSIAQRTMQCLPSLVPLNTESLGSNAMRKSPDTKLSPKGVINFIQTHGRITGNIPEVQAEDNTGNINVTDSRNTVTASVLISSNVSNPGTKSQDSNLFLQKSRNLKNTLNNIKAMRTGQVSLLKGAQNRNPIEDVRPLVTTTLVPQYGGEPSVEEALPEHMIFAAPKKKREDEEQFLQTALPQLKKTVPSQSKTKSTKLDLTISVDSHVSPPSTSISNVKSLTDAIFLGSVIRDPDLLMLILKALKWPVTAENCDEQMSRLKSSKFAVIMSDTNLLQDTDLTQLLGPYLAPMLPVVQQQDQHPLSSAIGSASSNHSSQATKDVLKLADLSSAMPYKLPPETSVQLVPSSPTEQEPQPVRHKKAVSVPTVKRHQRKMRALDLPAVDKTRRIISLSTSKAAYVSNELSNINSVLFSQFESNPADAINEALISLLKQQQQETKDQRKTRQSCIGSDSAVNLEDIVLIDPQPPLAVDPHNVKYLEDDYVSPQSQPGSQYNFKRRKTDIRSSEPSEVVVLDNSGSPNEKNSKEVFPSTKQTLSRETKKSAVTVRKESLGQLKQAPTSTVPEDICDPKARAINAKENPEVCEPVPDNGGSDDIDDSKAGSVSVRKSEVKAALGQKLLEAIGLSQLGADAAPENSRDTLRSALKRSIKQAQEQQLQLKRGNHEEPVKQLADSTTKLSAAESADEHKKAIAERELALLNRGFKAGEENTISLKDDKWERTDVSSSSSRNRRGRKSKIDSNVEDFGDEEKKDSRWDEDDDLPLKADTEKPSERNSTNARPTRTSKTLSKYYKGPTSKSHHSMGTRSTRQR

Target ORF information:

RefSeq Version	NM_001258880.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster debra (dbr), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258880.2

ORF Insert Sequence:

ATGGATGCTC AGTTTGAGCA CTTGTGCCGC ATTTGCGCTG CCAACACGAA AAGCAAGACG 
AATTCCGTGG TTGAAAGTGT GTTTATTTTT AAAACACAGG GACTGAAGGA TAAAATATCT 
CGACACCTTT ATCTAAATGT AGCTGAAGAA GATCCTCTGC CAAAAGTGCT CTGCAAATCT 
TGTTACCGCC AAGTAGAGGC CACGGCGTCG CTTTCAAATA TAGCAAAACA TACTCAGCTC 
GTGTTTCGGG ACTTTCTCCT TAGTACACTG CCTAAGAATG CGAGGGAAGC GGCTGCTGCA 
CAACTGAGCG TTCCAGCAAC CCAAATCCCA CACCGACACG ACGAAGTCCA AGCGGAAATC 
GATGAATTTC GCCCCATTTT GCTGACCCAC GTGCCGGAGT CCGGAAACAC CGCTTCCAAG 
CCCCAACCAG CCAATTGCCT GGAGCTGGCC CCAAAAACCG GCGAAAACTA CTTGGCTGCA 
TCTTCCTCAT CCTCTTCTCT GAGCAAACCC GCTGGATCAG TAAAGAGCTA TCTTAACACT 
GGGAGACGCA ACAGCGTTTT TACAGACACT AGGTATGGAT CGGCCCCACT GTCTATCGCT 
CAACGAACTA TGCAGTGTCT GCCTTCTTTG GTGCCCCTAA ACACAGAGTC ATTGGGTAGC 
AACGCAATGA GAAAGAGCCC AGACACAAAA CTATCGCCGA AAGGAGTAAT AAACTTCATC 
CAGACTCATG GGCGCATCAC GGGCAATATA CCTGAAGTCC AAGCAGAGGA CAATACCGGA 
AACATCAACG TGACAGATAG CAGAAACACA GTTACAGCTT CCGTTCTTAT TTCTTCAAAC 
GTTAGCAATC CAGGAACGAA ATCTCAGGAC AGTAATTTGT TTCTGCAGAA AAGTAGAAAT 
CTTAAAAACA CATTAAACAA CATTAAAGCC ATGCGTACCG GACAGGTATC ACTGCTTAAG 
GGTGCGCAAA ATCGAAACCC TATTGAAGAT GTTAGACCAC TGGTCACAAC TACACTGGTA 
CCCCAATATG GTGGTGAACC TTCCGTGGAG GAAGCACTGC CAGAGCACAT GATCTTTGCG 
GCGCCAAAAA AGAAAAGGGA GGATGAAGAA CAATTTCTTC AAACGGCTCT GCCACAGCTA 
AAGAAAACTG TGCCAAGTCA ATCTAAGACA AAGTCCACAA AACTCGATTT AACTATATCG 
GTTGATTCGC ATGTTTCACC CCCGTCAACG TCAATTTCTA ATGTTAAAAG CCTCACTGAT 
GCAATATTCC TGGGAAGCGT TATTAGGGAT CCAGACTTGT TGATGCTTAT TCTCAAGGCT 
CTAAAATGGC CGGTGACAGC AGAAAACTGT GATGAGCAAA TGTCCCGCTT AAAAAGTTCA 
AAGTTCGCTG TTATAATGTC AGACACAAAC CTTCTTCAAG ACACAGATCT CACACAACTT 
TTAGGTCCAT ATTTGGCCCC AATGCTGCCT GTGGTTCAGC AACAAGATCA GCATCCACTT 
TCCTCAGCTA TCGGGTCTGC TTCTTCAAAC CATTCATCGC AGGCGACTAA AGATGTACTT 
AAGCTGGCTG ACTTATCTAG TGCAATGCCT TATAAACTTC CACCAGAAAC GTCGGTTCAA 
TTAGTGCCTT CCAGTCCCAC GGAACAGGAA CCGCAGCCTG TGCGTCACAA AAAGGCGGTT 
AGCGTGCCAA CTGTCAAACG CCATCAACGA AAGATGCGTG CCCTTGATTT ACCTGCTGTT 
GACAAGACGC GCCGCATAAT TTCGTTATCG ACTTCAAAGG CAGCTTATGT CAGCAACGAA 
CTGTCGAATA TCAATTCTGT GCTTTTTTCG CAGTTCGAAT CAAATCCGGC CGACGCTATT 
AATGAAGCGC TTATCTCACT CCTTAAGCAG CAACAACAAG AAACAAAAGA CCAGCGAAAA 
ACTCGTCAGA GTTGTATTGG CTCTGACAGT GCAGTTAATT TAGAAGACAT TGTACTGATC 
GATCCCCAAC CGCCTCTTGC AGTCGATCCG CATAATGTGA AGTACCTTGA GGATGACTAC 
GTCTCTCCGC AGTCCCAGCC CGGATCCCAA TACAATTTTA AAAGGCGCAA AACAGACATT 
CGGTCATCAG AGCCGTCTGA AGTCGTTGTG CTCGATAACA GCGGATCCCC AAATGAAAAG 
AATTCAAAAG AAGTATTTCC AAGTACGAAA CAGACTTTAT CCAGAGAAAC AAAGAAAAGT 
GCGGTAACTG TTAGAAAAGA ATCTTTGGGT CAGCTAAAGC AGGCACCCAC AAGTACCGTC 
CCGGAAGACA TTTGCGATCC TAAAGCCAGA GCCATTAATG CGAAAGAAAA TCCCGAAGTG 
TGTGAACCAG TCCCGGACAA TGGAGGTAGT GACGATATTG ATGATAGCAA AGCAGGCTCG 
GTATCCGTTC GGAAGTCGGA AGTCAAAGCT GCGCTTGGAC AAAAGCTTTT GGAGGCTATT 
GGACTTTCTC AGTTGGGAGC CGATGCAGCG CCTGAAAACT CCCGCGACAC TCTTAGAAGT 
GCGTTAAAGC GCTCTATAAA ACAGGCCCAG GAGCAACAGC TTCAGCTGAA GCGAGGCAAT 
CATGAAGAAC CTGTGAAGCA ACTTGCCGAT TCCACAACCA AGCTAAGTGC AGCAGAATCG 
GCAGATGAGC ATAAAAAAGC AATTGCCGAG CGGGAGCTGG CCCTGCTGAA TCGTGGATTT 
AAAGCAGGAG AAGAAAACAC GATTTCCCTA AAAGATGATA AGTGGGAGCG AACTGATGTT 
AGTTCTTCAT CATCAAGAAA CCGCCGAGGA CGTAAATCAA AGATTGATTC CAACGTCGAG 
GATTTTGGCG ATGAAGAAAA AAAAGACTCT AGATGGGATG AAGACGATGA CTTGCCGTTA 
AAGGCAGATA CGGAAAAACC TTCAGAACGG AACAGCACCA ACGCCCGACC GACCCGTACT 
AGCAAAACGT TATCAAAGTA TTATAAAGGA CCAACTTCTA AATCACATCA TAGCATGGGT 
ACGCGCTCGA CACGACAGCG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dbr ( NM_134642.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_608486.1 Drosophila melanogaster debra (dbr), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa13297
Clone ID Related Accession (Same CDS sequence)	NM_001258880.2 , NM_134642.3 , NM_001258882.2 , NM_001258883.3 , NM_001258881.2
Accession Version	NM_134642.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3024bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	debra, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_134642.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATGCTC AGTTTGAGCA CTTGTGCCGC ATTTGCGCTG CCAACACGAA AAGCAAGACG 
AATTCCGTGG TTGAAAGTGT GTTTATTTTT AAAACACAGG GACTGAAGGA TAAAATATCT 
CGACACCTTT ATCTAAATGT AGCTGAAGAA GATCCTCTGC CAAAAGTGCT CTGCAAATCT 
TGTTACCGCC AAGTAGAGGC CACGGCGTCG CTTTCAAATA TAGCAAAACA TACTCAGCTC 
GTGTTTCGGG ACTTTCTCCT TAGTACACTG CCTAAGAATG CGAGGGAAGC GGCTGCTGCA 
CAACTGAGCG TTCCAGCAAC CCAAATCCCA CACCGACACG ACGAAGTCCA AGCGGAAATC 
GATGAATTTC GCCCCATTTT GCTGACCCAC GTGCCGGAGT CCGGAAACAC CGCTTCCAAG 
CCCCAACCAG CCAATTGCCT GGAGCTGGCC CCAAAAACCG GCGAAAACTA CTTGGCTGCA 
TCTTCCTCAT CCTCTTCTCT GAGCAAACCC GCTGGATCAG TAAAGAGCTA TCTTAACACT 
GGGAGACGCA ACAGCGTTTT TACAGACACT AGGTATGGAT CGGCCCCACT GTCTATCGCT 
CAACGAACTA TGCAGTGTCT GCCTTCTTTG GTGCCCCTAA ACACAGAGTC ATTGGGTAGC 
AACGCAATGA GAAAGAGCCC AGACACAAAA CTATCGCCGA AAGGAGTAAT AAACTTCATC 
CAGACTCATG GGCGCATCAC GGGCAATATA CCTGAAGTCC AAGCAGAGGA CAATACCGGA 
AACATCAACG TGACAGATAG CAGAAACACA GTTACAGCTT CCGTTCTTAT TTCTTCAAAC 
GTTAGCAATC CAGGAACGAA ATCTCAGGAC AGTAATTTGT TTCTGCAGAA AAGTAGAAAT 
CTTAAAAACA CATTAAACAA CATTAAAGCC ATGCGTACCG GACAGGTATC ACTGCTTAAG 
GGTGCGCAAA ATCGAAACCC TATTGAAGAT GTTAGACCAC TGGTCACAAC TACACTGGTA 
CCCCAATATG GTGGTGAACC TTCCGTGGAG GAAGCACTGC CAGAGCACAT GATCTTTGCG 
GCGCCAAAAA AGAAAAGGGA GGATGAAGAA CAATTTCTTC AAACGGCTCT GCCACAGCTA 
AAGAAAACTG TGCCAAGTCA ATCTAAGACA AAGTCCACAA AACTCGATTT AACTATATCG 
GTTGATTCGC ATGTTTCACC CCCGTCAACG TCAATTTCTA ATGTTAAAAG CCTCACTGAT 
GCAATATTCC TGGGAAGCGT TATTAGGGAT CCAGACTTGT TGATGCTTAT TCTCAAGGCT 
CTAAAATGGC CGGTGACAGC AGAAAACTGT GATGAGCAAA TGTCCCGCTT AAAAAGTTCA 
AAGTTCGCTG TTATAATGTC AGACACAAAC CTTCTTCAAG ACACAGATCT CACACAACTT 
TTAGGTCCAT ATTTGGCCCC AATGCTGCCT GTGGTTCAGC AACAAGATCA GCATCCACTT 
TCCTCAGCTA TCGGGTCTGC TTCTTCAAAC CATTCATCGC AGGCGACTAA AGATGTACTT 
AAGCTGGCTG ACTTATCTAG TGCAATGCCT TATAAACTTC CACCAGAAAC GTCGGTTCAA 
TTAGTGCCTT CCAGTCCCAC GGAACAGGAA CCGCAGCCTG TGCGTCACAA AAAGGCGGTT 
AGCGTGCCAA CTGTCAAACG CCATCAACGA AAGATGCGTG CCCTTGATTT ACCTGCTGTT 
GACAAGACGC GCCGCATAAT TTCGTTATCG ACTTCAAAGG CAGCTTATGT CAGCAACGAA 
CTGTCGAATA TCAATTCTGT GCTTTTTTCG CAGTTCGAAT CAAATCCGGC CGACGCTATT 
AATGAAGCGC TTATCTCACT CCTTAAGCAG CAACAACAAG AAACAAAAGA CCAGCGAAAA 
ACTCGTCAGA GTTGTATTGG CTCTGACAGT GCAGTTAATT TAGAAGACAT TGTACTGATC 
GATCCCCAAC CGCCTCTTGC AGTCGATCCG CATAATGTGA AGTACCTTGA GGATGACTAC 
GTCTCTCCGC AGTCCCAGCC CGGATCCCAA TACAATTTTA AAAGGCGCAA AACAGACATT 
CGGTCATCAG AGCCGTCTGA AGTCGTTGTG CTCGATAACA GCGGATCCCC AAATGAAAAG 
AATTCAAAAG AAGTATTTCC AAGTACGAAA CAGACTTTAT CCAGAGAAAC AAAGAAAAGT 
GCGGTAACTG TTAGAAAAGA ATCTTTGGGT CAGCTAAAGC AGGCACCCAC AAGTACCGTC 
CCGGAAGACA TTTGCGATCC TAAAGCCAGA GCCATTAATG CGAAAGAAAA TCCCGAAGTG 
TGTGAACCAG TCCCGGACAA TGGAGGTAGT GACGATATTG ATGATAGCAA AGCAGGCTCG 
GTATCCGTTC GGAAGTCGGA AGTCAAAGCT GCGCTTGGAC AAAAGCTTTT GGAGGCTATT 
GGACTTTCTC AGTTGGGAGC CGATGCAGCG CCTGAAAACT CCCGCGACAC TCTTAGAAGT 
GCGTTAAAGC GCTCTATAAA ACAGGCCCAG GAGCAACAGC TTCAGCTGAA GCGAGGCAAT 
CATGAAGAAC CTGTGAAGCA ACTTGCCGAT TCCACAACCA AGCTAAGTGC AGCAGAATCG 
GCAGATGAGC ATAAAAAAGC AATTGCCGAG CGGGAGCTGG CCCTGCTGAA TCGTGGATTT 
AAAGCAGGAG AAGAAAACAC GATTTCCCTA AAAGATGATA AGTGGGAGCG AACTGATGTT 
AGTTCTTCAT CATCAAGAAA CCGCCGAGGA CGTAAATCAA AGATTGATTC CAACGTCGAG 
GATTTTGGCG ATGAAGAAAA AAAAGACTCT AGATGGGATG AAGACGATGA CTTGCCGTTA 
AAGGCAGATA CGGAAAAACC TTCAGAACGG AACAGCACCA ACGCCCGACC GACCCGTACT 
AGCAAAACGT TATCAAAGTA TTATAAAGGA CCAACTTCTA AATCACATCA TAGCATGGGT 
ACGCGCTCGA CACGACAGCG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_608486.1
CDS	522..3545
Translation	MDAQFEHLCRICAANTKSKTNSVVESVFIFKTQGLKDKISRHLYLNVAEEDPLPKVLCKSCYRQVEATASLSNIAKHTQLVFRDFLLSTLPKNAREAAAAQLSVPATQIPHRHDEVQAEIDEFRPILLTHVPESGNTASKPQPANCLELAPKTGENYLAASSSSSSLSKPAGSVKSYLNTGRRNSVFTDTRYGSAPLSIAQRTMQCLPSLVPLNTESLGSNAMRKSPDTKLSPKGVINFIQTHGRITGNIPEVQAEDNTGNINVTDSRNTVTASVLISSNVSNPGTKSQDSNLFLQKSRNLKNTLNNIKAMRTGQVSLLKGAQNRNPIEDVRPLVTTTLVPQYGGEPSVEEALPEHMIFAAPKKKREDEEQFLQTALPQLKKTVPSQSKTKSTKLDLTISVDSHVSPPSTSISNVKSLTDAIFLGSVIRDPDLLMLILKALKWPVTAENCDEQMSRLKSSKFAVIMSDTNLLQDTDLTQLLGPYLAPMLPVVQQQDQHPLSSAIGSASSNHSSQATKDVLKLADLSSAMPYKLPPETSVQLVPSSPTEQEPQPVRHKKAVSVPTVKRHQRKMRALDLPAVDKTRRIISLSTSKAAYVSNELSNINSVLFSQFESNPADAINEALISLLKQQQQETKDQRKTRQSCIGSDSAVNLEDIVLIDPQPPLAVDPHNVKYLEDDYVSPQSQPGSQYNFKRRKTDIRSSEPSEVVVLDNSGSPNEKNSKEVFPSTKQTLSRETKKSAVTVRKESLGQLKQAPTSTVPEDICDPKARAINAKENPEVCEPVPDNGGSDDIDDSKAGSVSVRKSEVKAALGQKLLEAIGLSQLGADAAPENSRDTLRSALKRSIKQAQEQQLQLKRGNHEEPVKQLADSTTKLSAAESADEHKKAIAERELALLNRGFKAGEENTISLKDDKWERTDVSSSSSRNRRGRKSKIDSNVEDFGDEEKKDSRWDEDDDLPLKADTEKPSERNSTNARPTRTSKTLSKYYKGPTSKSHHSMGTRSTRQR

Target ORF information:

RefSeq Version	NM_134642.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster debra (dbr), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134642.3

ORF Insert Sequence:

ATGGATGCTC AGTTTGAGCA CTTGTGCCGC ATTTGCGCTG CCAACACGAA AAGCAAGACG 
AATTCCGTGG TTGAAAGTGT GTTTATTTTT AAAACACAGG GACTGAAGGA TAAAATATCT 
CGACACCTTT ATCTAAATGT AGCTGAAGAA GATCCTCTGC CAAAAGTGCT CTGCAAATCT 
TGTTACCGCC AAGTAGAGGC CACGGCGTCG CTTTCAAATA TAGCAAAACA TACTCAGCTC 
GTGTTTCGGG ACTTTCTCCT TAGTACACTG CCTAAGAATG CGAGGGAAGC GGCTGCTGCA 
CAACTGAGCG TTCCAGCAAC CCAAATCCCA CACCGACACG ACGAAGTCCA AGCGGAAATC 
GATGAATTTC GCCCCATTTT GCTGACCCAC GTGCCGGAGT CCGGAAACAC CGCTTCCAAG 
CCCCAACCAG CCAATTGCCT GGAGCTGGCC CCAAAAACCG GCGAAAACTA CTTGGCTGCA 
TCTTCCTCAT CCTCTTCTCT GAGCAAACCC GCTGGATCAG TAAAGAGCTA TCTTAACACT 
GGGAGACGCA ACAGCGTTTT TACAGACACT AGGTATGGAT CGGCCCCACT GTCTATCGCT 
CAACGAACTA TGCAGTGTCT GCCTTCTTTG GTGCCCCTAA ACACAGAGTC ATTGGGTAGC 
AACGCAATGA GAAAGAGCCC AGACACAAAA CTATCGCCGA AAGGAGTAAT AAACTTCATC 
CAGACTCATG GGCGCATCAC GGGCAATATA CCTGAAGTCC AAGCAGAGGA CAATACCGGA 
AACATCAACG TGACAGATAG CAGAAACACA GTTACAGCTT CCGTTCTTAT TTCTTCAAAC 
GTTAGCAATC CAGGAACGAA ATCTCAGGAC AGTAATTTGT TTCTGCAGAA AAGTAGAAAT 
CTTAAAAACA CATTAAACAA CATTAAAGCC ATGCGTACCG GACAGGTATC ACTGCTTAAG 
GGTGCGCAAA ATCGAAACCC TATTGAAGAT GTTAGACCAC TGGTCACAAC TACACTGGTA 
CCCCAATATG GTGGTGAACC TTCCGTGGAG GAAGCACTGC CAGAGCACAT GATCTTTGCG 
GCGCCAAAAA AGAAAAGGGA GGATGAAGAA CAATTTCTTC AAACGGCTCT GCCACAGCTA 
AAGAAAACTG TGCCAAGTCA ATCTAAGACA AAGTCCACAA AACTCGATTT AACTATATCG 
GTTGATTCGC ATGTTTCACC CCCGTCAACG TCAATTTCTA ATGTTAAAAG CCTCACTGAT 
GCAATATTCC TGGGAAGCGT TATTAGGGAT CCAGACTTGT TGATGCTTAT TCTCAAGGCT 
CTAAAATGGC CGGTGACAGC AGAAAACTGT GATGAGCAAA TGTCCCGCTT AAAAAGTTCA 
AAGTTCGCTG TTATAATGTC AGACACAAAC CTTCTTCAAG ACACAGATCT CACACAACTT 
TTAGGTCCAT ATTTGGCCCC AATGCTGCCT GTGGTTCAGC AACAAGATCA GCATCCACTT 
TCCTCAGCTA TCGGGTCTGC TTCTTCAAAC CATTCATCGC AGGCGACTAA AGATGTACTT 
AAGCTGGCTG ACTTATCTAG TGCAATGCCT TATAAACTTC CACCAGAAAC GTCGGTTCAA 
TTAGTGCCTT CCAGTCCCAC GGAACAGGAA CCGCAGCCTG TGCGTCACAA AAAGGCGGTT 
AGCGTGCCAA CTGTCAAACG CCATCAACGA AAGATGCGTG CCCTTGATTT ACCTGCTGTT 
GACAAGACGC GCCGCATAAT TTCGTTATCG ACTTCAAAGG CAGCTTATGT CAGCAACGAA 
CTGTCGAATA TCAATTCTGT GCTTTTTTCG CAGTTCGAAT CAAATCCGGC CGACGCTATT 
AATGAAGCGC TTATCTCACT CCTTAAGCAG CAACAACAAG AAACAAAAGA CCAGCGAAAA 
ACTCGTCAGA GTTGTATTGG CTCTGACAGT GCAGTTAATT TAGAAGACAT TGTACTGATC 
GATCCCCAAC CGCCTCTTGC AGTCGATCCG CATAATGTGA AGTACCTTGA GGATGACTAC 
GTCTCTCCGC AGTCCCAGCC CGGATCCCAA TACAATTTTA AAAGGCGCAA AACAGACATT 
CGGTCATCAG AGCCGTCTGA AGTCGTTGTG CTCGATAACA GCGGATCCCC AAATGAAAAG 
AATTCAAAAG AAGTATTTCC AAGTACGAAA CAGACTTTAT CCAGAGAAAC AAAGAAAAGT 
GCGGTAACTG TTAGAAAAGA ATCTTTGGGT CAGCTAAAGC AGGCACCCAC AAGTACCGTC 
CCGGAAGACA TTTGCGATCC TAAAGCCAGA GCCATTAATG CGAAAGAAAA TCCCGAAGTG 
TGTGAACCAG TCCCGGACAA TGGAGGTAGT GACGATATTG ATGATAGCAA AGCAGGCTCG 
GTATCCGTTC GGAAGTCGGA AGTCAAAGCT GCGCTTGGAC AAAAGCTTTT GGAGGCTATT 
GGACTTTCTC AGTTGGGAGC CGATGCAGCG CCTGAAAACT CCCGCGACAC TCTTAGAAGT 
GCGTTAAAGC GCTCTATAAA ACAGGCCCAG GAGCAACAGC TTCAGCTGAA GCGAGGCAAT 
CATGAAGAAC CTGTGAAGCA ACTTGCCGAT TCCACAACCA AGCTAAGTGC AGCAGAATCG 
GCAGATGAGC ATAAAAAAGC AATTGCCGAG CGGGAGCTGG CCCTGCTGAA TCGTGGATTT 
AAAGCAGGAG AAGAAAACAC GATTTCCCTA AAAGATGATA AGTGGGAGCG AACTGATGTT 
AGTTCTTCAT CATCAAGAAA CCGCCGAGGA CGTAAATCAA AGATTGATTC CAACGTCGAG 
GATTTTGGCG ATGAAGAAAA AAAAGACTCT AGATGGGATG AAGACGATGA CTTGCCGTTA 
AAGGCAGATA CGGAAAAACC TTCAGAACGG AACAGCACCA ACGCCCGACC GACCCGTACT 
AGCAAAACGT TATCAAAGTA TTATAAAGGA CCAACTTCTA AATCACATCA TAGCATGGGT 
ACGCGCTCGA CACGACAGCG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dbr ( NM_001258882.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245811.1 Drosophila melanogaster debra (dbr), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa13297
Clone ID Related Accession (Same CDS sequence)	NM_001258880.2 , NM_134642.3 , NM_001258882.2 , NM_001258883.3 , NM_001258881.2
Accession Version	NM_001258882.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3024bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	debra, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001258882.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATGCTC AGTTTGAGCA CTTGTGCCGC ATTTGCGCTG CCAACACGAA AAGCAAGACG 
AATTCCGTGG TTGAAAGTGT GTTTATTTTT AAAACACAGG GACTGAAGGA TAAAATATCT 
CGACACCTTT ATCTAAATGT AGCTGAAGAA GATCCTCTGC CAAAAGTGCT CTGCAAATCT 
TGTTACCGCC AAGTAGAGGC CACGGCGTCG CTTTCAAATA TAGCAAAACA TACTCAGCTC 
GTGTTTCGGG ACTTTCTCCT TAGTACACTG CCTAAGAATG CGAGGGAAGC GGCTGCTGCA 
CAACTGAGCG TTCCAGCAAC CCAAATCCCA CACCGACACG ACGAAGTCCA AGCGGAAATC 
GATGAATTTC GCCCCATTTT GCTGACCCAC GTGCCGGAGT CCGGAAACAC CGCTTCCAAG 
CCCCAACCAG CCAATTGCCT GGAGCTGGCC CCAAAAACCG GCGAAAACTA CTTGGCTGCA 
TCTTCCTCAT CCTCTTCTCT GAGCAAACCC GCTGGATCAG TAAAGAGCTA TCTTAACACT 
GGGAGACGCA ACAGCGTTTT TACAGACACT AGGTATGGAT CGGCCCCACT GTCTATCGCT 
CAACGAACTA TGCAGTGTCT GCCTTCTTTG GTGCCCCTAA ACACAGAGTC ATTGGGTAGC 
AACGCAATGA GAAAGAGCCC AGACACAAAA CTATCGCCGA AAGGAGTAAT AAACTTCATC 
CAGACTCATG GGCGCATCAC GGGCAATATA CCTGAAGTCC AAGCAGAGGA CAATACCGGA 
AACATCAACG TGACAGATAG CAGAAACACA GTTACAGCTT CCGTTCTTAT TTCTTCAAAC 
GTTAGCAATC CAGGAACGAA ATCTCAGGAC AGTAATTTGT TTCTGCAGAA AAGTAGAAAT 
CTTAAAAACA CATTAAACAA CATTAAAGCC ATGCGTACCG GACAGGTATC ACTGCTTAAG 
GGTGCGCAAA ATCGAAACCC TATTGAAGAT GTTAGACCAC TGGTCACAAC TACACTGGTA 
CCCCAATATG GTGGTGAACC TTCCGTGGAG GAAGCACTGC CAGAGCACAT GATCTTTGCG 
GCGCCAAAAA AGAAAAGGGA GGATGAAGAA CAATTTCTTC AAACGGCTCT GCCACAGCTA 
AAGAAAACTG TGCCAAGTCA ATCTAAGACA AAGTCCACAA AACTCGATTT AACTATATCG 
GTTGATTCGC ATGTTTCACC CCCGTCAACG TCAATTTCTA ATGTTAAAAG CCTCACTGAT 
GCAATATTCC TGGGAAGCGT TATTAGGGAT CCAGACTTGT TGATGCTTAT TCTCAAGGCT 
CTAAAATGGC CGGTGACAGC AGAAAACTGT GATGAGCAAA TGTCCCGCTT AAAAAGTTCA 
AAGTTCGCTG TTATAATGTC AGACACAAAC CTTCTTCAAG ACACAGATCT CACACAACTT 
TTAGGTCCAT ATTTGGCCCC AATGCTGCCT GTGGTTCAGC AACAAGATCA GCATCCACTT 
TCCTCAGCTA TCGGGTCTGC TTCTTCAAAC CATTCATCGC AGGCGACTAA AGATGTACTT 
AAGCTGGCTG ACTTATCTAG TGCAATGCCT TATAAACTTC CACCAGAAAC GTCGGTTCAA 
TTAGTGCCTT CCAGTCCCAC GGAACAGGAA CCGCAGCCTG TGCGTCACAA AAAGGCGGTT 
AGCGTGCCAA CTGTCAAACG CCATCAACGA AAGATGCGTG CCCTTGATTT ACCTGCTGTT 
GACAAGACGC GCCGCATAAT TTCGTTATCG ACTTCAAAGG CAGCTTATGT CAGCAACGAA 
CTGTCGAATA TCAATTCTGT GCTTTTTTCG CAGTTCGAAT CAAATCCGGC CGACGCTATT 
AATGAAGCGC TTATCTCACT CCTTAAGCAG CAACAACAAG AAACAAAAGA CCAGCGAAAA 
ACTCGTCAGA GTTGTATTGG CTCTGACAGT GCAGTTAATT TAGAAGACAT TGTACTGATC 
GATCCCCAAC CGCCTCTTGC AGTCGATCCG CATAATGTGA AGTACCTTGA GGATGACTAC 
GTCTCTCCGC AGTCCCAGCC CGGATCCCAA TACAATTTTA AAAGGCGCAA AACAGACATT 
CGGTCATCAG AGCCGTCTGA AGTCGTTGTG CTCGATAACA GCGGATCCCC AAATGAAAAG 
AATTCAAAAG AAGTATTTCC AAGTACGAAA CAGACTTTAT CCAGAGAAAC AAAGAAAAGT 
GCGGTAACTG TTAGAAAAGA ATCTTTGGGT CAGCTAAAGC AGGCACCCAC AAGTACCGTC 
CCGGAAGACA TTTGCGATCC TAAAGCCAGA GCCATTAATG CGAAAGAAAA TCCCGAAGTG 
TGTGAACCAG TCCCGGACAA TGGAGGTAGT GACGATATTG ATGATAGCAA AGCAGGCTCG 
GTATCCGTTC GGAAGTCGGA AGTCAAAGCT GCGCTTGGAC AAAAGCTTTT GGAGGCTATT 
GGACTTTCTC AGTTGGGAGC CGATGCAGCG CCTGAAAACT CCCGCGACAC TCTTAGAAGT 
GCGTTAAAGC GCTCTATAAA ACAGGCCCAG GAGCAACAGC TTCAGCTGAA GCGAGGCAAT 
CATGAAGAAC CTGTGAAGCA ACTTGCCGAT TCCACAACCA AGCTAAGTGC AGCAGAATCG 
GCAGATGAGC ATAAAAAAGC AATTGCCGAG CGGGAGCTGG CCCTGCTGAA TCGTGGATTT 
AAAGCAGGAG AAGAAAACAC GATTTCCCTA AAAGATGATA AGTGGGAGCG AACTGATGTT 
AGTTCTTCAT CATCAAGAAA CCGCCGAGGA CGTAAATCAA AGATTGATTC CAACGTCGAG 
GATTTTGGCG ATGAAGAAAA AAAAGACTCT AGATGGGATG AAGACGATGA CTTGCCGTTA 
AAGGCAGATA CGGAAAAACC TTCAGAACGG AACAGCACCA ACGCCCGACC GACCCGTACT 
AGCAAAACGT TATCAAAGTA TTATAAAGGA CCAACTTCTA AATCACATCA TAGCATGGGT 
ACGCGCTCGA CACGACAGCG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245811.1
CDS	245..3268
Translation	MDAQFEHLCRICAANTKSKTNSVVESVFIFKTQGLKDKISRHLYLNVAEEDPLPKVLCKSCYRQVEATASLSNIAKHTQLVFRDFLLSTLPKNAREAAAAQLSVPATQIPHRHDEVQAEIDEFRPILLTHVPESGNTASKPQPANCLELAPKTGENYLAASSSSSSLSKPAGSVKSYLNTGRRNSVFTDTRYGSAPLSIAQRTMQCLPSLVPLNTESLGSNAMRKSPDTKLSPKGVINFIQTHGRITGNIPEVQAEDNTGNINVTDSRNTVTASVLISSNVSNPGTKSQDSNLFLQKSRNLKNTLNNIKAMRTGQVSLLKGAQNRNPIEDVRPLVTTTLVPQYGGEPSVEEALPEHMIFAAPKKKREDEEQFLQTALPQLKKTVPSQSKTKSTKLDLTISVDSHVSPPSTSISNVKSLTDAIFLGSVIRDPDLLMLILKALKWPVTAENCDEQMSRLKSSKFAVIMSDTNLLQDTDLTQLLGPYLAPMLPVVQQQDQHPLSSAIGSASSNHSSQATKDVLKLADLSSAMPYKLPPETSVQLVPSSPTEQEPQPVRHKKAVSVPTVKRHQRKMRALDLPAVDKTRRIISLSTSKAAYVSNELSNINSVLFSQFESNPADAINEALISLLKQQQQETKDQRKTRQSCIGSDSAVNLEDIVLIDPQPPLAVDPHNVKYLEDDYVSPQSQPGSQYNFKRRKTDIRSSEPSEVVVLDNSGSPNEKNSKEVFPSTKQTLSRETKKSAVTVRKESLGQLKQAPTSTVPEDICDPKARAINAKENPEVCEPVPDNGGSDDIDDSKAGSVSVRKSEVKAALGQKLLEAIGLSQLGADAAPENSRDTLRSALKRSIKQAQEQQLQLKRGNHEEPVKQLADSTTKLSAAESADEHKKAIAERELALLNRGFKAGEENTISLKDDKWERTDVSSSSSRNRRGRKSKIDSNVEDFGDEEKKDSRWDEDDDLPLKADTEKPSERNSTNARPTRTSKTLSKYYKGPTSKSHHSMGTRSTRQR

Target ORF information:

RefSeq Version	NM_001258882.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster debra (dbr), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258882.2

ORF Insert Sequence:

ATGGATGCTC AGTTTGAGCA CTTGTGCCGC ATTTGCGCTG CCAACACGAA AAGCAAGACG 
AATTCCGTGG TTGAAAGTGT GTTTATTTTT AAAACACAGG GACTGAAGGA TAAAATATCT 
CGACACCTTT ATCTAAATGT AGCTGAAGAA GATCCTCTGC CAAAAGTGCT CTGCAAATCT 
TGTTACCGCC AAGTAGAGGC CACGGCGTCG CTTTCAAATA TAGCAAAACA TACTCAGCTC 
GTGTTTCGGG ACTTTCTCCT TAGTACACTG CCTAAGAATG CGAGGGAAGC GGCTGCTGCA 
CAACTGAGCG TTCCAGCAAC CCAAATCCCA CACCGACACG ACGAAGTCCA AGCGGAAATC 
GATGAATTTC GCCCCATTTT GCTGACCCAC GTGCCGGAGT CCGGAAACAC CGCTTCCAAG 
CCCCAACCAG CCAATTGCCT GGAGCTGGCC CCAAAAACCG GCGAAAACTA CTTGGCTGCA 
TCTTCCTCAT CCTCTTCTCT GAGCAAACCC GCTGGATCAG TAAAGAGCTA TCTTAACACT 
GGGAGACGCA ACAGCGTTTT TACAGACACT AGGTATGGAT CGGCCCCACT GTCTATCGCT 
CAACGAACTA TGCAGTGTCT GCCTTCTTTG GTGCCCCTAA ACACAGAGTC ATTGGGTAGC 
AACGCAATGA GAAAGAGCCC AGACACAAAA CTATCGCCGA AAGGAGTAAT AAACTTCATC 
CAGACTCATG GGCGCATCAC GGGCAATATA CCTGAAGTCC AAGCAGAGGA CAATACCGGA 
AACATCAACG TGACAGATAG CAGAAACACA GTTACAGCTT CCGTTCTTAT TTCTTCAAAC 
GTTAGCAATC CAGGAACGAA ATCTCAGGAC AGTAATTTGT TTCTGCAGAA AAGTAGAAAT 
CTTAAAAACA CATTAAACAA CATTAAAGCC ATGCGTACCG GACAGGTATC ACTGCTTAAG 
GGTGCGCAAA ATCGAAACCC TATTGAAGAT GTTAGACCAC TGGTCACAAC TACACTGGTA 
CCCCAATATG GTGGTGAACC TTCCGTGGAG GAAGCACTGC CAGAGCACAT GATCTTTGCG 
GCGCCAAAAA AGAAAAGGGA GGATGAAGAA CAATTTCTTC AAACGGCTCT GCCACAGCTA 
AAGAAAACTG TGCCAAGTCA ATCTAAGACA AAGTCCACAA AACTCGATTT AACTATATCG 
GTTGATTCGC ATGTTTCACC CCCGTCAACG TCAATTTCTA ATGTTAAAAG CCTCACTGAT 
GCAATATTCC TGGGAAGCGT TATTAGGGAT CCAGACTTGT TGATGCTTAT TCTCAAGGCT 
CTAAAATGGC CGGTGACAGC AGAAAACTGT GATGAGCAAA TGTCCCGCTT AAAAAGTTCA 
AAGTTCGCTG TTATAATGTC AGACACAAAC CTTCTTCAAG ACACAGATCT CACACAACTT 
TTAGGTCCAT ATTTGGCCCC AATGCTGCCT GTGGTTCAGC AACAAGATCA GCATCCACTT 
TCCTCAGCTA TCGGGTCTGC TTCTTCAAAC CATTCATCGC AGGCGACTAA AGATGTACTT 
AAGCTGGCTG ACTTATCTAG TGCAATGCCT TATAAACTTC CACCAGAAAC GTCGGTTCAA 
TTAGTGCCTT CCAGTCCCAC GGAACAGGAA CCGCAGCCTG TGCGTCACAA AAAGGCGGTT 
AGCGTGCCAA CTGTCAAACG CCATCAACGA AAGATGCGTG CCCTTGATTT ACCTGCTGTT 
GACAAGACGC GCCGCATAAT TTCGTTATCG ACTTCAAAGG CAGCTTATGT CAGCAACGAA 
CTGTCGAATA TCAATTCTGT GCTTTTTTCG CAGTTCGAAT CAAATCCGGC CGACGCTATT 
AATGAAGCGC TTATCTCACT CCTTAAGCAG CAACAACAAG AAACAAAAGA CCAGCGAAAA 
ACTCGTCAGA GTTGTATTGG CTCTGACAGT GCAGTTAATT TAGAAGACAT TGTACTGATC 
GATCCCCAAC CGCCTCTTGC AGTCGATCCG CATAATGTGA AGTACCTTGA GGATGACTAC 
GTCTCTCCGC AGTCCCAGCC CGGATCCCAA TACAATTTTA AAAGGCGCAA AACAGACATT 
CGGTCATCAG AGCCGTCTGA AGTCGTTGTG CTCGATAACA GCGGATCCCC AAATGAAAAG 
AATTCAAAAG AAGTATTTCC AAGTACGAAA CAGACTTTAT CCAGAGAAAC AAAGAAAAGT 
GCGGTAACTG TTAGAAAAGA ATCTTTGGGT CAGCTAAAGC AGGCACCCAC AAGTACCGTC 
CCGGAAGACA TTTGCGATCC TAAAGCCAGA GCCATTAATG CGAAAGAAAA TCCCGAAGTG 
TGTGAACCAG TCCCGGACAA TGGAGGTAGT GACGATATTG ATGATAGCAA AGCAGGCTCG 
GTATCCGTTC GGAAGTCGGA AGTCAAAGCT GCGCTTGGAC AAAAGCTTTT GGAGGCTATT 
GGACTTTCTC AGTTGGGAGC CGATGCAGCG CCTGAAAACT CCCGCGACAC TCTTAGAAGT 
GCGTTAAAGC GCTCTATAAA ACAGGCCCAG GAGCAACAGC TTCAGCTGAA GCGAGGCAAT 
CATGAAGAAC CTGTGAAGCA ACTTGCCGAT TCCACAACCA AGCTAAGTGC AGCAGAATCG 
GCAGATGAGC ATAAAAAAGC AATTGCCGAG CGGGAGCTGG CCCTGCTGAA TCGTGGATTT 
AAAGCAGGAG AAGAAAACAC GATTTCCCTA AAAGATGATA AGTGGGAGCG AACTGATGTT 
AGTTCTTCAT CATCAAGAAA CCGCCGAGGA CGTAAATCAA AGATTGATTC CAACGTCGAG 
GATTTTGGCG ATGAAGAAAA AAAAGACTCT AGATGGGATG AAGACGATGA CTTGCCGTTA 
AAGGCAGATA CGGAAAAACC TTCAGAACGG AACAGCACCA ACGCCCGACC GACCCGTACT 
AGCAAAACGT TATCAAAGTA TTATAAAGGA CCAACTTCTA AATCACATCA TAGCATGGGT 
ACGCGCTCGA CACGACAGCG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dbr ( NM_001258883.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245812.1 Drosophila melanogaster debra (dbr), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa13297
Clone ID Related Accession (Same CDS sequence)	NM_001258880.2 , NM_134642.3 , NM_001258882.2 , NM_001258883.3 , NM_001258881.2
Accession Version	NM_001258883.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3024bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	debra, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001258883.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATGCTC AGTTTGAGCA CTTGTGCCGC ATTTGCGCTG CCAACACGAA AAGCAAGACG 
AATTCCGTGG TTGAAAGTGT GTTTATTTTT AAAACACAGG GACTGAAGGA TAAAATATCT 
CGACACCTTT ATCTAAATGT AGCTGAAGAA GATCCTCTGC CAAAAGTGCT CTGCAAATCT 
TGTTACCGCC AAGTAGAGGC CACGGCGTCG CTTTCAAATA TAGCAAAACA TACTCAGCTC 
GTGTTTCGGG ACTTTCTCCT TAGTACACTG CCTAAGAATG CGAGGGAAGC GGCTGCTGCA 
CAACTGAGCG TTCCAGCAAC CCAAATCCCA CACCGACACG ACGAAGTCCA AGCGGAAATC 
GATGAATTTC GCCCCATTTT GCTGACCCAC GTGCCGGAGT CCGGAAACAC CGCTTCCAAG 
CCCCAACCAG CCAATTGCCT GGAGCTGGCC CCAAAAACCG GCGAAAACTA CTTGGCTGCA 
TCTTCCTCAT CCTCTTCTCT GAGCAAACCC GCTGGATCAG TAAAGAGCTA TCTTAACACT 
GGGAGACGCA ACAGCGTTTT TACAGACACT AGGTATGGAT CGGCCCCACT GTCTATCGCT 
CAACGAACTA TGCAGTGTCT GCCTTCTTTG GTGCCCCTAA ACACAGAGTC ATTGGGTAGC 
AACGCAATGA GAAAGAGCCC AGACACAAAA CTATCGCCGA AAGGAGTAAT AAACTTCATC 
CAGACTCATG GGCGCATCAC GGGCAATATA CCTGAAGTCC AAGCAGAGGA CAATACCGGA 
AACATCAACG TGACAGATAG CAGAAACACA GTTACAGCTT CCGTTCTTAT TTCTTCAAAC 
GTTAGCAATC CAGGAACGAA ATCTCAGGAC AGTAATTTGT TTCTGCAGAA AAGTAGAAAT 
CTTAAAAACA CATTAAACAA CATTAAAGCC ATGCGTACCG GACAGGTATC ACTGCTTAAG 
GGTGCGCAAA ATCGAAACCC TATTGAAGAT GTTAGACCAC TGGTCACAAC TACACTGGTA 
CCCCAATATG GTGGTGAACC TTCCGTGGAG GAAGCACTGC CAGAGCACAT GATCTTTGCG 
GCGCCAAAAA AGAAAAGGGA GGATGAAGAA CAATTTCTTC AAACGGCTCT GCCACAGCTA 
AAGAAAACTG TGCCAAGTCA ATCTAAGACA AAGTCCACAA AACTCGATTT AACTATATCG 
GTTGATTCGC ATGTTTCACC CCCGTCAACG TCAATTTCTA ATGTTAAAAG CCTCACTGAT 
GCAATATTCC TGGGAAGCGT TATTAGGGAT CCAGACTTGT TGATGCTTAT TCTCAAGGCT 
CTAAAATGGC CGGTGACAGC AGAAAACTGT GATGAGCAAA TGTCCCGCTT AAAAAGTTCA 
AAGTTCGCTG TTATAATGTC AGACACAAAC CTTCTTCAAG ACACAGATCT CACACAACTT 
TTAGGTCCAT ATTTGGCCCC AATGCTGCCT GTGGTTCAGC AACAAGATCA GCATCCACTT 
TCCTCAGCTA TCGGGTCTGC TTCTTCAAAC CATTCATCGC AGGCGACTAA AGATGTACTT 
AAGCTGGCTG ACTTATCTAG TGCAATGCCT TATAAACTTC CACCAGAAAC GTCGGTTCAA 
TTAGTGCCTT CCAGTCCCAC GGAACAGGAA CCGCAGCCTG TGCGTCACAA AAAGGCGGTT 
AGCGTGCCAA CTGTCAAACG CCATCAACGA AAGATGCGTG CCCTTGATTT ACCTGCTGTT 
GACAAGACGC GCCGCATAAT TTCGTTATCG ACTTCAAAGG CAGCTTATGT CAGCAACGAA 
CTGTCGAATA TCAATTCTGT GCTTTTTTCG CAGTTCGAAT CAAATCCGGC CGACGCTATT 
AATGAAGCGC TTATCTCACT CCTTAAGCAG CAACAACAAG AAACAAAAGA CCAGCGAAAA 
ACTCGTCAGA GTTGTATTGG CTCTGACAGT GCAGTTAATT TAGAAGACAT TGTACTGATC 
GATCCCCAAC CGCCTCTTGC AGTCGATCCG CATAATGTGA AGTACCTTGA GGATGACTAC 
GTCTCTCCGC AGTCCCAGCC CGGATCCCAA TACAATTTTA AAAGGCGCAA AACAGACATT 
CGGTCATCAG AGCCGTCTGA AGTCGTTGTG CTCGATAACA GCGGATCCCC AAATGAAAAG 
AATTCAAAAG AAGTATTTCC AAGTACGAAA CAGACTTTAT CCAGAGAAAC AAAGAAAAGT 
GCGGTAACTG TTAGAAAAGA ATCTTTGGGT CAGCTAAAGC AGGCACCCAC AAGTACCGTC 
CCGGAAGACA TTTGCGATCC TAAAGCCAGA GCCATTAATG CGAAAGAAAA TCCCGAAGTG 
TGTGAACCAG TCCCGGACAA TGGAGGTAGT GACGATATTG ATGATAGCAA AGCAGGCTCG 
GTATCCGTTC GGAAGTCGGA AGTCAAAGCT GCGCTTGGAC AAAAGCTTTT GGAGGCTATT 
GGACTTTCTC AGTTGGGAGC CGATGCAGCG CCTGAAAACT CCCGCGACAC TCTTAGAAGT 
GCGTTAAAGC GCTCTATAAA ACAGGCCCAG GAGCAACAGC TTCAGCTGAA GCGAGGCAAT 
CATGAAGAAC CTGTGAAGCA ACTTGCCGAT TCCACAACCA AGCTAAGTGC AGCAGAATCG 
GCAGATGAGC ATAAAAAAGC AATTGCCGAG CGGGAGCTGG CCCTGCTGAA TCGTGGATTT 
AAAGCAGGAG AAGAAAACAC GATTTCCCTA AAAGATGATA AGTGGGAGCG AACTGATGTT 
AGTTCTTCAT CATCAAGAAA CCGCCGAGGA CGTAAATCAA AGATTGATTC CAACGTCGAG 
GATTTTGGCG ATGAAGAAAA AAAAGACTCT AGATGGGATG AAGACGATGA CTTGCCGTTA 
AAGGCAGATA CGGAAAAACC TTCAGAACGG AACAGCACCA ACGCCCGACC GACCCGTACT 
AGCAAAACGT TATCAAAGTA TTATAAAGGA CCAACTTCTA AATCACATCA TAGCATGGGT 
ACGCGCTCGA CACGACAGCG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245812.1
CDS	635..3658
Translation	MDAQFEHLCRICAANTKSKTNSVVESVFIFKTQGLKDKISRHLYLNVAEEDPLPKVLCKSCYRQVEATASLSNIAKHTQLVFRDFLLSTLPKNAREAAAAQLSVPATQIPHRHDEVQAEIDEFRPILLTHVPESGNTASKPQPANCLELAPKTGENYLAASSSSSSLSKPAGSVKSYLNTGRRNSVFTDTRYGSAPLSIAQRTMQCLPSLVPLNTESLGSNAMRKSPDTKLSPKGVINFIQTHGRITGNIPEVQAEDNTGNINVTDSRNTVTASVLISSNVSNPGTKSQDSNLFLQKSRNLKNTLNNIKAMRTGQVSLLKGAQNRNPIEDVRPLVTTTLVPQYGGEPSVEEALPEHMIFAAPKKKREDEEQFLQTALPQLKKTVPSQSKTKSTKLDLTISVDSHVSPPSTSISNVKSLTDAIFLGSVIRDPDLLMLILKALKWPVTAENCDEQMSRLKSSKFAVIMSDTNLLQDTDLTQLLGPYLAPMLPVVQQQDQHPLSSAIGSASSNHSSQATKDVLKLADLSSAMPYKLPPETSVQLVPSSPTEQEPQPVRHKKAVSVPTVKRHQRKMRALDLPAVDKTRRIISLSTSKAAYVSNELSNINSVLFSQFESNPADAINEALISLLKQQQQETKDQRKTRQSCIGSDSAVNLEDIVLIDPQPPLAVDPHNVKYLEDDYVSPQSQPGSQYNFKRRKTDIRSSEPSEVVVLDNSGSPNEKNSKEVFPSTKQTLSRETKKSAVTVRKESLGQLKQAPTSTVPEDICDPKARAINAKENPEVCEPVPDNGGSDDIDDSKAGSVSVRKSEVKAALGQKLLEAIGLSQLGADAAPENSRDTLRSALKRSIKQAQEQQLQLKRGNHEEPVKQLADSTTKLSAAESADEHKKAIAERELALLNRGFKAGEENTISLKDDKWERTDVSSSSSRNRRGRKSKIDSNVEDFGDEEKKDSRWDEDDDLPLKADTEKPSERNSTNARPTRTSKTLSKYYKGPTSKSHHSMGTRSTRQR

Target ORF information:

RefSeq Version	NM_001258883.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster debra (dbr), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258883.3

ORF Insert Sequence:

ATGGATGCTC AGTTTGAGCA CTTGTGCCGC ATTTGCGCTG CCAACACGAA AAGCAAGACG 
AATTCCGTGG TTGAAAGTGT GTTTATTTTT AAAACACAGG GACTGAAGGA TAAAATATCT 
CGACACCTTT ATCTAAATGT AGCTGAAGAA GATCCTCTGC CAAAAGTGCT CTGCAAATCT 
TGTTACCGCC AAGTAGAGGC CACGGCGTCG CTTTCAAATA TAGCAAAACA TACTCAGCTC 
GTGTTTCGGG ACTTTCTCCT TAGTACACTG CCTAAGAATG CGAGGGAAGC GGCTGCTGCA 
CAACTGAGCG TTCCAGCAAC CCAAATCCCA CACCGACACG ACGAAGTCCA AGCGGAAATC 
GATGAATTTC GCCCCATTTT GCTGACCCAC GTGCCGGAGT CCGGAAACAC CGCTTCCAAG 
CCCCAACCAG CCAATTGCCT GGAGCTGGCC CCAAAAACCG GCGAAAACTA CTTGGCTGCA 
TCTTCCTCAT CCTCTTCTCT GAGCAAACCC GCTGGATCAG TAAAGAGCTA TCTTAACACT 
GGGAGACGCA ACAGCGTTTT TACAGACACT AGGTATGGAT CGGCCCCACT GTCTATCGCT 
CAACGAACTA TGCAGTGTCT GCCTTCTTTG GTGCCCCTAA ACACAGAGTC ATTGGGTAGC 
AACGCAATGA GAAAGAGCCC AGACACAAAA CTATCGCCGA AAGGAGTAAT AAACTTCATC 
CAGACTCATG GGCGCATCAC GGGCAATATA CCTGAAGTCC AAGCAGAGGA CAATACCGGA 
AACATCAACG TGACAGATAG CAGAAACACA GTTACAGCTT CCGTTCTTAT TTCTTCAAAC 
GTTAGCAATC CAGGAACGAA ATCTCAGGAC AGTAATTTGT TTCTGCAGAA AAGTAGAAAT 
CTTAAAAACA CATTAAACAA CATTAAAGCC ATGCGTACCG GACAGGTATC ACTGCTTAAG 
GGTGCGCAAA ATCGAAACCC TATTGAAGAT GTTAGACCAC TGGTCACAAC TACACTGGTA 
CCCCAATATG GTGGTGAACC TTCCGTGGAG GAAGCACTGC CAGAGCACAT GATCTTTGCG 
GCGCCAAAAA AGAAAAGGGA GGATGAAGAA CAATTTCTTC AAACGGCTCT GCCACAGCTA 
AAGAAAACTG TGCCAAGTCA ATCTAAGACA AAGTCCACAA AACTCGATTT AACTATATCG 
GTTGATTCGC ATGTTTCACC CCCGTCAACG TCAATTTCTA ATGTTAAAAG CCTCACTGAT 
GCAATATTCC TGGGAAGCGT TATTAGGGAT CCAGACTTGT TGATGCTTAT TCTCAAGGCT 
CTAAAATGGC CGGTGACAGC AGAAAACTGT GATGAGCAAA TGTCCCGCTT AAAAAGTTCA 
AAGTTCGCTG TTATAATGTC AGACACAAAC CTTCTTCAAG ACACAGATCT CACACAACTT 
TTAGGTCCAT ATTTGGCCCC AATGCTGCCT GTGGTTCAGC AACAAGATCA GCATCCACTT 
TCCTCAGCTA TCGGGTCTGC TTCTTCAAAC CATTCATCGC AGGCGACTAA AGATGTACTT 
AAGCTGGCTG ACTTATCTAG TGCAATGCCT TATAAACTTC CACCAGAAAC GTCGGTTCAA 
TTAGTGCCTT CCAGTCCCAC GGAACAGGAA CCGCAGCCTG TGCGTCACAA AAAGGCGGTT 
AGCGTGCCAA CTGTCAAACG CCATCAACGA AAGATGCGTG CCCTTGATTT ACCTGCTGTT 
GACAAGACGC GCCGCATAAT TTCGTTATCG ACTTCAAAGG CAGCTTATGT CAGCAACGAA 
CTGTCGAATA TCAATTCTGT GCTTTTTTCG CAGTTCGAAT CAAATCCGGC CGACGCTATT 
AATGAAGCGC TTATCTCACT CCTTAAGCAG CAACAACAAG AAACAAAAGA CCAGCGAAAA 
ACTCGTCAGA GTTGTATTGG CTCTGACAGT GCAGTTAATT TAGAAGACAT TGTACTGATC 
GATCCCCAAC CGCCTCTTGC AGTCGATCCG CATAATGTGA AGTACCTTGA GGATGACTAC 
GTCTCTCCGC AGTCCCAGCC CGGATCCCAA TACAATTTTA AAAGGCGCAA AACAGACATT 
CGGTCATCAG AGCCGTCTGA AGTCGTTGTG CTCGATAACA GCGGATCCCC AAATGAAAAG 
AATTCAAAAG AAGTATTTCC AAGTACGAAA CAGACTTTAT CCAGAGAAAC AAAGAAAAGT 
GCGGTAACTG TTAGAAAAGA ATCTTTGGGT CAGCTAAAGC AGGCACCCAC AAGTACCGTC 
CCGGAAGACA TTTGCGATCC TAAAGCCAGA GCCATTAATG CGAAAGAAAA TCCCGAAGTG 
TGTGAACCAG TCCCGGACAA TGGAGGTAGT GACGATATTG ATGATAGCAA AGCAGGCTCG 
GTATCCGTTC GGAAGTCGGA AGTCAAAGCT GCGCTTGGAC AAAAGCTTTT GGAGGCTATT 
GGACTTTCTC AGTTGGGAGC CGATGCAGCG CCTGAAAACT CCCGCGACAC TCTTAGAAGT 
GCGTTAAAGC GCTCTATAAA ACAGGCCCAG GAGCAACAGC TTCAGCTGAA GCGAGGCAAT 
CATGAAGAAC CTGTGAAGCA ACTTGCCGAT TCCACAACCA AGCTAAGTGC AGCAGAATCG 
GCAGATGAGC ATAAAAAAGC AATTGCCGAG CGGGAGCTGG CCCTGCTGAA TCGTGGATTT 
AAAGCAGGAG AAGAAAACAC GATTTCCCTA AAAGATGATA AGTGGGAGCG AACTGATGTT 
AGTTCTTCAT CATCAAGAAA CCGCCGAGGA CGTAAATCAA AGATTGATTC CAACGTCGAG 
GATTTTGGCG ATGAAGAAAA AAAAGACTCT AGATGGGATG AAGACGATGA CTTGCCGTTA 
AAGGCAGATA CGGAAAAACC TTCAGAACGG AACAGCACCA ACGCCCGACC GACCCGTACT 
AGCAAAACGT TATCAAAGTA TTATAAAGGA CCAACTTCTA AATCACATCA TAGCATGGGT 
ACGCGCTCGA CACGACAGCG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dbr ( NM_001258881.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245810.1 Drosophila melanogaster debra (dbr), transcript variant D, mRNA.

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CloneID	OFa13297
Clone ID Related Accession (Same CDS sequence)	NM_001258880.2 , NM_134642.3 , NM_001258882.2 , NM_001258883.3 , NM_001258881.2
Accession Version	NM_001258881.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3024bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	debra, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001258881.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATGCTC AGTTTGAGCA CTTGTGCCGC ATTTGCGCTG CCAACACGAA AAGCAAGACG 
AATTCCGTGG TTGAAAGTGT GTTTATTTTT AAAACACAGG GACTGAAGGA TAAAATATCT 
CGACACCTTT ATCTAAATGT AGCTGAAGAA GATCCTCTGC CAAAAGTGCT CTGCAAATCT 
TGTTACCGCC AAGTAGAGGC CACGGCGTCG CTTTCAAATA TAGCAAAACA TACTCAGCTC 
GTGTTTCGGG ACTTTCTCCT TAGTACACTG CCTAAGAATG CGAGGGAAGC GGCTGCTGCA 
CAACTGAGCG TTCCAGCAAC CCAAATCCCA CACCGACACG ACGAAGTCCA AGCGGAAATC 
GATGAATTTC GCCCCATTTT GCTGACCCAC GTGCCGGAGT CCGGAAACAC CGCTTCCAAG 
CCCCAACCAG CCAATTGCCT GGAGCTGGCC CCAAAAACCG GCGAAAACTA CTTGGCTGCA 
TCTTCCTCAT CCTCTTCTCT GAGCAAACCC GCTGGATCAG TAAAGAGCTA TCTTAACACT 
GGGAGACGCA ACAGCGTTTT TACAGACACT AGGTATGGAT CGGCCCCACT GTCTATCGCT 
CAACGAACTA TGCAGTGTCT GCCTTCTTTG GTGCCCCTAA ACACAGAGTC ATTGGGTAGC 
AACGCAATGA GAAAGAGCCC AGACACAAAA CTATCGCCGA AAGGAGTAAT AAACTTCATC 
CAGACTCATG GGCGCATCAC GGGCAATATA CCTGAAGTCC AAGCAGAGGA CAATACCGGA 
AACATCAACG TGACAGATAG CAGAAACACA GTTACAGCTT CCGTTCTTAT TTCTTCAAAC 
GTTAGCAATC CAGGAACGAA ATCTCAGGAC AGTAATTTGT TTCTGCAGAA AAGTAGAAAT 
CTTAAAAACA CATTAAACAA CATTAAAGCC ATGCGTACCG GACAGGTATC ACTGCTTAAG 
GGTGCGCAAA ATCGAAACCC TATTGAAGAT GTTAGACCAC TGGTCACAAC TACACTGGTA 
CCCCAATATG GTGGTGAACC TTCCGTGGAG GAAGCACTGC CAGAGCACAT GATCTTTGCG 
GCGCCAAAAA AGAAAAGGGA GGATGAAGAA CAATTTCTTC AAACGGCTCT GCCACAGCTA 
AAGAAAACTG TGCCAAGTCA ATCTAAGACA AAGTCCACAA AACTCGATTT AACTATATCG 
GTTGATTCGC ATGTTTCACC CCCGTCAACG TCAATTTCTA ATGTTAAAAG CCTCACTGAT 
GCAATATTCC TGGGAAGCGT TATTAGGGAT CCAGACTTGT TGATGCTTAT TCTCAAGGCT 
CTAAAATGGC CGGTGACAGC AGAAAACTGT GATGAGCAAA TGTCCCGCTT AAAAAGTTCA 
AAGTTCGCTG TTATAATGTC AGACACAAAC CTTCTTCAAG ACACAGATCT CACACAACTT 
TTAGGTCCAT ATTTGGCCCC AATGCTGCCT GTGGTTCAGC AACAAGATCA GCATCCACTT 
TCCTCAGCTA TCGGGTCTGC TTCTTCAAAC CATTCATCGC AGGCGACTAA AGATGTACTT 
AAGCTGGCTG ACTTATCTAG TGCAATGCCT TATAAACTTC CACCAGAAAC GTCGGTTCAA 
TTAGTGCCTT CCAGTCCCAC GGAACAGGAA CCGCAGCCTG TGCGTCACAA AAAGGCGGTT 
AGCGTGCCAA CTGTCAAACG CCATCAACGA AAGATGCGTG CCCTTGATTT ACCTGCTGTT 
GACAAGACGC GCCGCATAAT TTCGTTATCG ACTTCAAAGG CAGCTTATGT CAGCAACGAA 
CTGTCGAATA TCAATTCTGT GCTTTTTTCG CAGTTCGAAT CAAATCCGGC CGACGCTATT 
AATGAAGCGC TTATCTCACT CCTTAAGCAG CAACAACAAG AAACAAAAGA CCAGCGAAAA 
ACTCGTCAGA GTTGTATTGG CTCTGACAGT GCAGTTAATT TAGAAGACAT TGTACTGATC 
GATCCCCAAC CGCCTCTTGC AGTCGATCCG CATAATGTGA AGTACCTTGA GGATGACTAC 
GTCTCTCCGC AGTCCCAGCC CGGATCCCAA TACAATTTTA AAAGGCGCAA AACAGACATT 
CGGTCATCAG AGCCGTCTGA AGTCGTTGTG CTCGATAACA GCGGATCCCC AAATGAAAAG 
AATTCAAAAG AAGTATTTCC AAGTACGAAA CAGACTTTAT CCAGAGAAAC AAAGAAAAGT 
GCGGTAACTG TTAGAAAAGA ATCTTTGGGT CAGCTAAAGC AGGCACCCAC AAGTACCGTC 
CCGGAAGACA TTTGCGATCC TAAAGCCAGA GCCATTAATG CGAAAGAAAA TCCCGAAGTG 
TGTGAACCAG TCCCGGACAA TGGAGGTAGT GACGATATTG ATGATAGCAA AGCAGGCTCG 
GTATCCGTTC GGAAGTCGGA AGTCAAAGCT GCGCTTGGAC AAAAGCTTTT GGAGGCTATT 
GGACTTTCTC AGTTGGGAGC CGATGCAGCG CCTGAAAACT CCCGCGACAC TCTTAGAAGT 
GCGTTAAAGC GCTCTATAAA ACAGGCCCAG GAGCAACAGC TTCAGCTGAA GCGAGGCAAT 
CATGAAGAAC CTGTGAAGCA ACTTGCCGAT TCCACAACCA AGCTAAGTGC AGCAGAATCG 
GCAGATGAGC ATAAAAAAGC AATTGCCGAG CGGGAGCTGG CCCTGCTGAA TCGTGGATTT 
AAAGCAGGAG AAGAAAACAC GATTTCCCTA AAAGATGATA AGTGGGAGCG AACTGATGTT 
AGTTCTTCAT CATCAAGAAA CCGCCGAGGA CGTAAATCAA AGATTGATTC CAACGTCGAG 
GATTTTGGCG ATGAAGAAAA AAAAGACTCT AGATGGGATG AAGACGATGA CTTGCCGTTA 
AAGGCAGATA CGGAAAAACC TTCAGAACGG AACAGCACCA ACGCCCGACC GACCCGTACT 
AGCAAAACGT TATCAAAGTA TTATAAAGGA CCAACTTCTA AATCACATCA TAGCATGGGT 
ACGCGCTCGA CACGACAGCG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245810.1
CDS	271..3294
Translation	MDAQFEHLCRICAANTKSKTNSVVESVFIFKTQGLKDKISRHLYLNVAEEDPLPKVLCKSCYRQVEATASLSNIAKHTQLVFRDFLLSTLPKNAREAAAAQLSVPATQIPHRHDEVQAEIDEFRPILLTHVPESGNTASKPQPANCLELAPKTGENYLAASSSSSSLSKPAGSVKSYLNTGRRNSVFTDTRYGSAPLSIAQRTMQCLPSLVPLNTESLGSNAMRKSPDTKLSPKGVINFIQTHGRITGNIPEVQAEDNTGNINVTDSRNTVTASVLISSNVSNPGTKSQDSNLFLQKSRNLKNTLNNIKAMRTGQVSLLKGAQNRNPIEDVRPLVTTTLVPQYGGEPSVEEALPEHMIFAAPKKKREDEEQFLQTALPQLKKTVPSQSKTKSTKLDLTISVDSHVSPPSTSISNVKSLTDAIFLGSVIRDPDLLMLILKALKWPVTAENCDEQMSRLKSSKFAVIMSDTNLLQDTDLTQLLGPYLAPMLPVVQQQDQHPLSSAIGSASSNHSSQATKDVLKLADLSSAMPYKLPPETSVQLVPSSPTEQEPQPVRHKKAVSVPTVKRHQRKMRALDLPAVDKTRRIISLSTSKAAYVSNELSNINSVLFSQFESNPADAINEALISLLKQQQQETKDQRKTRQSCIGSDSAVNLEDIVLIDPQPPLAVDPHNVKYLEDDYVSPQSQPGSQYNFKRRKTDIRSSEPSEVVVLDNSGSPNEKNSKEVFPSTKQTLSRETKKSAVTVRKESLGQLKQAPTSTVPEDICDPKARAINAKENPEVCEPVPDNGGSDDIDDSKAGSVSVRKSEVKAALGQKLLEAIGLSQLGADAAPENSRDTLRSALKRSIKQAQEQQLQLKRGNHEEPVKQLADSTTKLSAAESADEHKKAIAERELALLNRGFKAGEENTISLKDDKWERTDVSSSSSRNRRGRKSKIDSNVEDFGDEEKKDSRWDEDDDLPLKADTEKPSERNSTNARPTRTSKTLSKYYKGPTSKSHHSMGTRSTRQR

Target ORF information:

RefSeq Version	NM_001258881.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster debra (dbr), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258881.2

ORF Insert Sequence:

ATGGATGCTC AGTTTGAGCA CTTGTGCCGC ATTTGCGCTG CCAACACGAA AAGCAAGACG 
AATTCCGTGG TTGAAAGTGT GTTTATTTTT AAAACACAGG GACTGAAGGA TAAAATATCT 
CGACACCTTT ATCTAAATGT AGCTGAAGAA GATCCTCTGC CAAAAGTGCT CTGCAAATCT 
TGTTACCGCC AAGTAGAGGC CACGGCGTCG CTTTCAAATA TAGCAAAACA TACTCAGCTC 
GTGTTTCGGG ACTTTCTCCT TAGTACACTG CCTAAGAATG CGAGGGAAGC GGCTGCTGCA 
CAACTGAGCG TTCCAGCAAC CCAAATCCCA CACCGACACG ACGAAGTCCA AGCGGAAATC 
GATGAATTTC GCCCCATTTT GCTGACCCAC GTGCCGGAGT CCGGAAACAC CGCTTCCAAG 
CCCCAACCAG CCAATTGCCT GGAGCTGGCC CCAAAAACCG GCGAAAACTA CTTGGCTGCA 
TCTTCCTCAT CCTCTTCTCT GAGCAAACCC GCTGGATCAG TAAAGAGCTA TCTTAACACT 
GGGAGACGCA ACAGCGTTTT TACAGACACT AGGTATGGAT CGGCCCCACT GTCTATCGCT 
CAACGAACTA TGCAGTGTCT GCCTTCTTTG GTGCCCCTAA ACACAGAGTC ATTGGGTAGC 
AACGCAATGA GAAAGAGCCC AGACACAAAA CTATCGCCGA AAGGAGTAAT AAACTTCATC 
CAGACTCATG GGCGCATCAC GGGCAATATA CCTGAAGTCC AAGCAGAGGA CAATACCGGA 
AACATCAACG TGACAGATAG CAGAAACACA GTTACAGCTT CCGTTCTTAT TTCTTCAAAC 
GTTAGCAATC CAGGAACGAA ATCTCAGGAC AGTAATTTGT TTCTGCAGAA AAGTAGAAAT 
CTTAAAAACA CATTAAACAA CATTAAAGCC ATGCGTACCG GACAGGTATC ACTGCTTAAG 
GGTGCGCAAA ATCGAAACCC TATTGAAGAT GTTAGACCAC TGGTCACAAC TACACTGGTA 
CCCCAATATG GTGGTGAACC TTCCGTGGAG GAAGCACTGC CAGAGCACAT GATCTTTGCG 
GCGCCAAAAA AGAAAAGGGA GGATGAAGAA CAATTTCTTC AAACGGCTCT GCCACAGCTA 
AAGAAAACTG TGCCAAGTCA ATCTAAGACA AAGTCCACAA AACTCGATTT AACTATATCG 
GTTGATTCGC ATGTTTCACC CCCGTCAACG TCAATTTCTA ATGTTAAAAG CCTCACTGAT 
GCAATATTCC TGGGAAGCGT TATTAGGGAT CCAGACTTGT TGATGCTTAT TCTCAAGGCT 
CTAAAATGGC CGGTGACAGC AGAAAACTGT GATGAGCAAA TGTCCCGCTT AAAAAGTTCA 
AAGTTCGCTG TTATAATGTC AGACACAAAC CTTCTTCAAG ACACAGATCT CACACAACTT 
TTAGGTCCAT ATTTGGCCCC AATGCTGCCT GTGGTTCAGC AACAAGATCA GCATCCACTT 
TCCTCAGCTA TCGGGTCTGC TTCTTCAAAC CATTCATCGC AGGCGACTAA AGATGTACTT 
AAGCTGGCTG ACTTATCTAG TGCAATGCCT TATAAACTTC CACCAGAAAC GTCGGTTCAA 
TTAGTGCCTT CCAGTCCCAC GGAACAGGAA CCGCAGCCTG TGCGTCACAA AAAGGCGGTT 
AGCGTGCCAA CTGTCAAACG CCATCAACGA AAGATGCGTG CCCTTGATTT ACCTGCTGTT 
GACAAGACGC GCCGCATAAT TTCGTTATCG ACTTCAAAGG CAGCTTATGT CAGCAACGAA 
CTGTCGAATA TCAATTCTGT GCTTTTTTCG CAGTTCGAAT CAAATCCGGC CGACGCTATT 
AATGAAGCGC TTATCTCACT CCTTAAGCAG CAACAACAAG AAACAAAAGA CCAGCGAAAA 
ACTCGTCAGA GTTGTATTGG CTCTGACAGT GCAGTTAATT TAGAAGACAT TGTACTGATC 
GATCCCCAAC CGCCTCTTGC AGTCGATCCG CATAATGTGA AGTACCTTGA GGATGACTAC 
GTCTCTCCGC AGTCCCAGCC CGGATCCCAA TACAATTTTA AAAGGCGCAA AACAGACATT 
CGGTCATCAG AGCCGTCTGA AGTCGTTGTG CTCGATAACA GCGGATCCCC AAATGAAAAG 
AATTCAAAAG AAGTATTTCC AAGTACGAAA CAGACTTTAT CCAGAGAAAC AAAGAAAAGT 
GCGGTAACTG TTAGAAAAGA ATCTTTGGGT CAGCTAAAGC AGGCACCCAC AAGTACCGTC 
CCGGAAGACA TTTGCGATCC TAAAGCCAGA GCCATTAATG CGAAAGAAAA TCCCGAAGTG 
TGTGAACCAG TCCCGGACAA TGGAGGTAGT GACGATATTG ATGATAGCAA AGCAGGCTCG 
GTATCCGTTC GGAAGTCGGA AGTCAAAGCT GCGCTTGGAC AAAAGCTTTT GGAGGCTATT 
GGACTTTCTC AGTTGGGAGC CGATGCAGCG CCTGAAAACT CCCGCGACAC TCTTAGAAGT 
GCGTTAAAGC GCTCTATAAA ACAGGCCCAG GAGCAACAGC TTCAGCTGAA GCGAGGCAAT 
CATGAAGAAC CTGTGAAGCA ACTTGCCGAT TCCACAACCA AGCTAAGTGC AGCAGAATCG 
GCAGATGAGC ATAAAAAAGC AATTGCCGAG CGGGAGCTGG CCCTGCTGAA TCGTGGATTT 
AAAGCAGGAG AAGAAAACAC GATTTCCCTA AAAGATGATA AGTGGGAGCG AACTGATGTT 
AGTTCTTCAT CATCAAGAAA CCGCCGAGGA CGTAAATCAA AGATTGATTC CAACGTCGAG 
GATTTTGGCG ATGAAGAAAA AAAAGACTCT AGATGGGATG AAGACGATGA CTTGCCGTTA 
AAGGCAGATA CGGAAAAACC TTCAGAACGG AACAGCACCA ACGCCCGACC GACCCGTACT 
AGCAAAACGT TATCAAAGTA TTATAAAGGA CCAACTTCTA AATCACATCA TAGCATGGGT 
ACGCGCTCGA CACGACAGCG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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dbr cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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