l(2)gl cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following l(2)gl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the l(2)gl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa13291 NM_001258872.2
Latest version!
Drosophila melanogaster lethal (2) giant larvae (l(2)gl), transcript variant G, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OFa13291 NM_164349.3
Latest version!
Drosophila melanogaster lethal (2) giant larvae (l(2)gl), transcript variant A, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OFa13291 NM_001258874.2
Latest version!
Drosophila melanogaster lethal (2) giant larvae (l(2)gl), transcript variant I, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OFa13291 NM_001258873.2
Latest version!
Drosophila melanogaster lethal (2) giant larvae (l(2)gl), transcript variant H, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OFa13291 NM_078715.4
Latest version!
Drosophila melanogaster lethal (2) giant larvae (l(2)gl), transcript variant C, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OFa13291 NM_001258875.2
Latest version!
Drosophila melanogaster lethal (2) giant larvae (l(2)gl), transcript variant J, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OFa13292 NM_001272858.1
Latest version!
Drosophila melanogaster lethal (2) giant larvae (l(2)gl), transcript variant K, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OFa15843 NM_164351.3
Latest version!
Drosophila melanogaster lethal (2) giant larvae (l(2)gl), transcript variant E, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OFa15843 NM_164352.3
Latest version!
Drosophila melanogaster lethal (2) giant larvae (l(2)gl), transcript variant F, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OFa15843 NM_164350.3
Latest version!
Drosophila melanogaster lethal (2) giant larvae (l(2)gl), transcript variant D, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OFa15852 NM_164348.3
Latest version!
Drosophila melanogaster lethal (2) giant larvae (l(2)gl), transcript variant B, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa13291
    Clone ID Related Accession (Same CDS sequence) NM_078715.4 , NM_164349.3 , NM_001258873.2 , NM_001258872.2 , NM_001258874.2 , NM_001258875.2
    Accession Version NM_078715.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3486bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product lethal (2) giant larvae, isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_078715.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTTAAAGT TTATCAGAGG AAAAGGGCAG CAGCCCAGTG CTGACAGACA CCGCCTACAG 
    AAGGACCTTT TTGCTTATCG TAAGACGGCA CAGCATGGCT TTCCTCATAA GCCTTCGGCT
    CTTGCGTATG ATCCAGTTTT GAAACTTATG GCAATAGGGA CGCAAACAGG GGCTTTAAAA
    GTTTTCGGTC AACCCGGAGT TGAATTGTAC GGTCAGCATA CTTTGTTAAA CAATTCAGCA
    TCGGAGCTTA ATGTACAATT ACTTGAATGG GTGTATGGAA CTGGTCGCAT ACTTTCGTTG
    ACGGCAGCGA ATCAATTAAT TCTATGGGAG CCAGTTGGAG CAACGTTGCT GCCAATCAAA
    ACACTACCGT TTGACGGCAA ACTTAAAAAA GTTTCATCGC TGTGCTGTTC TCTCAGTAAG
    GATCTGCTAT GGATTGGAAC AGAAGGTGGA AACATCTATC AACTGGATTT ACATACATTT
    ACCATTAAGG AGCCTGTAAT TTACCATGAC GTTGTGCTAG AGCAGGTGCC ACCAGCCTAC
    AAGCTAAATC CTGGTGCAAT TGAGTCAATC CGCCAACTTC CAAACTCCCC TAGCAAACTT
    CTAGTTGCAT ACAATCGCGG CCTTTGTGTT TTGTGGGATT TTGAAAGCGC ATCTGTCCAG
    CGAGCATACA TAGCCCCTGG ACATGGACAG AGCGTTGGTC TTACAGTGAA CTTCGAAGGA
    TCTGAATTTA CCTGGTACCA CGCTGATGGT TCATACGCCA CTTGGAGCAT AGATAACCCA
    GAACCGCCGT CGAATGTTAA TTATGTGCCT TATGGACCTG ATCCATGCAA AAGCATAAAT
    CGACTGTACA AAGGCAAGCG AAGATCCAAC GATGTAATTG TTTTTTCCGG CGGCATGCCA
    CGGTCAGCAT ATGGTGATCA CAATTGTGTG TCCGTTCACG CCAGCGATGG ACACAAAGTG
    TGTCTTGACT TTACGTCTAA AGTGATTGAC TTTTTTGTGA CCTTTGAAAA TAATAGAGAT
    GTCGCTGAAG TTCTTGTTGT ACTACTTGAA GAGGAACTCT GCGCTTACGA TCTTACTGAC
    CCTAATATTT GTGCTATCAA AGCGCCATAT CTTCACTCTG TCCATGCATC AGCTGTCACT
    TGCAATTACC TTGCTTCTGA AGTCGTACAG TCGGTATATG AAAGTATTTT AAGAGCTGGA
    GATGAACAAG ACATTGACTA TAGCAATATT AGCTGGCCTA TCACTGGCGG TACTCTCCCG
    GATAACTTAG AAGAATCTGT AGAAGAGGAC GCGACTAAGC TTTATGAGAT TTTGTTAACT
    GGTCACGAAG ATGGTTCTGT TAAATTTTGG GACTGCACTG GAGTGTTGCT TAAACCAATT
    TATAATTTTA AAACTAGCAG CATTTTTGGA AGTGAGTCAG ACTTCCGAGA TGACGCAGCT
    GCAGATATGA GTGCGGAACA AGTCGATGAA GGAGAACCGC CATTTCGGAA ATCAGGACTT
    TTTGATCCTT ATTCAGATGA CCCTCGTTTA GCAGTGAAGA AAATAGCATT CTGCCCAAAA
    ACCGGACAAC TTATTGTTGG TGGCACAGCG GGCCAAATAG TTATAGCCGA CTTCATAGAC
    TTACCCGAAA AAGTGTCTTT AAAATACATT TCAATGAATT TGGTCAGCGA TCGTGATGGA
    TTTGTGTGGA AGGGTCACGA TCAGTTAAAC GTGCGATCGA ACTTATTAGA CGGAGAAGCA
    ATTCCTACGA CGGAACGTGG TGTAAATATA TCGGGAGTAC TGCAAGTTTT GCCGCCAGCC
    AGCATAACAT GCATGGCACT CGAAGCAAGC TGGGGCCTAG TATCTGGTGG GACTGCGCAC
    GGCTTAGTTC TCTTTGACTT CAAAAACTTT GTTCCAGTAT TTCATCGCTG CACTTTAAAC
    CCAAATGATC TTACTGGAGC AGGAGAGCAG CTGTCTCGTC GAAAGTCTTT TAAGAAATCA
    TTGAGGGAGT CATTTAGAAA GCTTCGCAAG GGTCGATCGA CCAGGACCAA CCAGAGCAAT
    CAAGTACCAA CAACGCTGGA AGCAAGACCC GTCGAGAGGC AAATAGAGGC TCGTTGTGCA
    GATGACGGGC TAGGATCCAT GGTGCGATGT TTACTATTTG CCAAAACTTA TGTTACTAAT
    GTCAACATAA CGTCGCCAAC TTTGTGGTCA GCAACAAATG CCAGTACAGT CTCGGTTTTC
    CTTCTGCATT TGCCACCAGC GCAGACCGCG GCAACTGCCG TCCCGTCGGC AAGTGGCAAT
    GCACCACCAC ACATGCCCCG CCGAATTTCT GCGCAGCTTG CTAAAGAAAT ACAATTAAAA
    CATCGTGCTC CTGTGGTGGG TATTTCTATT TTTGATCAGG CGGGTAGCCC TGTCGATCAG
    CTGAACGCCG GTGAAAACGG GAGTCCACCG CATCGTGTAC TTATTGCTTC CGAGGAACAG
    TTCAAGGTGT TTTCACTTCC GCAACTAAAG CCGATTAACA AATATAAGCT TACCGCTAAC
    GAAGGTGCTC GGATTCGCCG CATCCATTTT GGTTCGTTTA GTTGTCGCAT ATCCCCGGAA
    ACACTGCAGA GTATGCACGG TTGTAGCCCA ACTAAGTCCA CGCGTTCACA TGGCGATGGA
    GAGGCGGATC CTAATATCAG TGGAAGCTTG GCTGTAAGTC GTGGAGATGT ATATAACGAA
    ACAGCATTGA TATGTTTAAC GAATATGGGC GATATCATGG TTTTATCAGT ACCTGAATTA
    AAAAGACAGC TGAATGCCGC AGCAGTGCGA CGGGAAGACA TTAATGGAGT TTCGTCACTT
    TGCTTTACAA ACTCTGGAGA AGCACTGTAT ATGATGTCTT CTTCTGAACT GCAGCGTATT
    GCTTTAGCCA CGTCCAGAGT CGTGCAACCC ACTGGCGTTG TTCCAGTAGA ACCATTAGAA
    AATGAAGAGT CTGTGTTGGA AGAAAATGAT GCAGAGAATA ATAAGGAAAC CTACGCATGT
    GATGAAGTTG TGAATACATA TGAAATTAAA AATCCATCAG GCATTTCAAT ATGCACAAGG
    CCTGCAGAGG AAAACGTTGG AAGAAATAGT GTTCAGCAAG TTAATGGAGT CAACATTTCA
    AATTCACCTA ATCAAGCTAA CGAGACTATC AGCAGCTCTA TTGGCGATAT TACCGTTGAC
    TCGGTGCGCG ACCATTTAAA TATGACGACC ACCACTTTGT GTTCTATTAA TACAGAGGAA
    ACCATTGGTC GCCTATCTGT ACTTAGCACG CAAACCAACA AAGCCAGTAC TACCGTAAAC
    ATGAGTGAAA TTCCAAATAT TAATATTTCT AATTTAGAGG ACTTGGAATC GAAAAGAAAT
    ACGACGGAAA CGAGTACTAG TTCTGTTGTA ATTAAATCTA TAATTACAAA CATTTCTCAT
    GAAAAAACGA ACGGAGACAA CAAAATAGGA ACGCCAAAAA CAGCGCCTGA AGAAAGCCAA
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_523439.2
    CDS387..3872
    Translation

    Target ORF information:

    RefSeq Version NM_078715.4
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster lethal (2) giant larvae (l(2)gl), transcript variant C, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_078715.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTTAAAGT TTATCAGAGG AAAAGGGCAG CAGCCCAGTG CTGACAGACA CCGCCTACAG 
    AAGGACCTTT TTGCTTATCG TAAGACGGCA CAGCATGGCT TTCCTCATAA GCCTTCGGCT
    CTTGCGTATG ATCCAGTTTT GAAACTTATG GCAATAGGGA CGCAAACAGG GGCTTTAAAA
    GTTTTCGGTC AACCCGGAGT TGAATTGTAC GGTCAGCATA CTTTGTTAAA CAATTCAGCA
    TCGGAGCTTA ATGTACAATT ACTTGAATGG GTGTATGGAA CTGGTCGCAT ACTTTCGTTG
    ACGGCAGCGA ATCAATTAAT TCTATGGGAG CCAGTTGGAG CAACGTTGCT GCCAATCAAA
    ACACTACCGT TTGACGGCAA ACTTAAAAAA GTTTCATCGC TGTGCTGTTC TCTCAGTAAG
    GATCTGCTAT GGATTGGAAC AGAAGGTGGA AACATCTATC AACTGGATTT ACATACATTT
    ACCATTAAGG AGCCTGTAAT TTACCATGAC GTTGTGCTAG AGCAGGTGCC ACCAGCCTAC
    AAGCTAAATC CTGGTGCAAT TGAGTCAATC CGCCAACTTC CAAACTCCCC TAGCAAACTT
    CTAGTTGCAT ACAATCGCGG CCTTTGTGTT TTGTGGGATT TTGAAAGCGC ATCTGTCCAG
    CGAGCATACA TAGCCCCTGG ACATGGACAG AGCGTTGGTC TTACAGTGAA CTTCGAAGGA
    TCTGAATTTA CCTGGTACCA CGCTGATGGT TCATACGCCA CTTGGAGCAT AGATAACCCA
    GAACCGCCGT CGAATGTTAA TTATGTGCCT TATGGACCTG ATCCATGCAA AAGCATAAAT
    CGACTGTACA AAGGCAAGCG AAGATCCAAC GATGTAATTG TTTTTTCCGG CGGCATGCCA
    CGGTCAGCAT ATGGTGATCA CAATTGTGTG TCCGTTCACG CCAGCGATGG ACACAAAGTG
    TGTCTTGACT TTACGTCTAA AGTGATTGAC TTTTTTGTGA CCTTTGAAAA TAATAGAGAT
    GTCGCTGAAG TTCTTGTTGT ACTACTTGAA GAGGAACTCT GCGCTTACGA TCTTACTGAC
    CCTAATATTT GTGCTATCAA AGCGCCATAT CTTCACTCTG TCCATGCATC AGCTGTCACT
    TGCAATTACC TTGCTTCTGA AGTCGTACAG TCGGTATATG AAAGTATTTT AAGAGCTGGA
    GATGAACAAG ACATTGACTA TAGCAATATT AGCTGGCCTA TCACTGGCGG TACTCTCCCG
    GATAACTTAG AAGAATCTGT AGAAGAGGAC GCGACTAAGC TTTATGAGAT TTTGTTAACT
    GGTCACGAAG ATGGTTCTGT TAAATTTTGG GACTGCACTG GAGTGTTGCT TAAACCAATT
    TATAATTTTA AAACTAGCAG CATTTTTGGA AGTGAGTCAG ACTTCCGAGA TGACGCAGCT
    GCAGATATGA GTGCGGAACA AGTCGATGAA GGAGAACCGC CATTTCGGAA ATCAGGACTT
    TTTGATCCTT ATTCAGATGA CCCTCGTTTA GCAGTGAAGA AAATAGCATT CTGCCCAAAA
    ACCGGACAAC TTATTGTTGG TGGCACAGCG GGCCAAATAG TTATAGCCGA CTTCATAGAC
    TTACCCGAAA AAGTGTCTTT AAAATACATT TCAATGAATT TGGTCAGCGA TCGTGATGGA
    TTTGTGTGGA AGGGTCACGA TCAGTTAAAC GTGCGATCGA ACTTATTAGA CGGAGAAGCA
    ATTCCTACGA CGGAACGTGG TGTAAATATA TCGGGAGTAC TGCAAGTTTT GCCGCCAGCC
    AGCATAACAT GCATGGCACT CGAAGCAAGC TGGGGCCTAG TATCTGGTGG GACTGCGCAC
    GGCTTAGTTC TCTTTGACTT CAAAAACTTT GTTCCAGTAT TTCATCGCTG CACTTTAAAC
    CCAAATGATC TTACTGGAGC AGGAGAGCAG CTGTCTCGTC GAAAGTCTTT TAAGAAATCA
    TTGAGGGAGT CATTTAGAAA GCTTCGCAAG GGTCGATCGA CCAGGACCAA CCAGAGCAAT
    CAAGTACCAA CAACGCTGGA AGCAAGACCC GTCGAGAGGC AAATAGAGGC TCGTTGTGCA
    GATGACGGGC TAGGATCCAT GGTGCGATGT TTACTATTTG CCAAAACTTA TGTTACTAAT
    GTCAACATAA CGTCGCCAAC TTTGTGGTCA GCAACAAATG CCAGTACAGT CTCGGTTTTC
    CTTCTGCATT TGCCACCAGC GCAGACCGCG GCAACTGCCG TCCCGTCGGC AAGTGGCAAT
    GCACCACCAC ACATGCCCCG CCGAATTTCT GCGCAGCTTG CTAAAGAAAT ACAATTAAAA
    CATCGTGCTC CTGTGGTGGG TATTTCTATT TTTGATCAGG CGGGTAGCCC TGTCGATCAG
    CTGAACGCCG GTGAAAACGG GAGTCCACCG CATCGTGTAC TTATTGCTTC CGAGGAACAG
    TTCAAGGTGT TTTCACTTCC GCAACTAAAG CCGATTAACA AATATAAGCT TACCGCTAAC
    GAAGGTGCTC GGATTCGCCG CATCCATTTT GGTTCGTTTA GTTGTCGCAT ATCCCCGGAA
    ACACTGCAGA GTATGCACGG TTGTAGCCCA ACTAAGTCCA CGCGTTCACA TGGCGATGGA
    GAGGCGGATC CTAATATCAG TGGAAGCTTG GCTGTAAGTC GTGGAGATGT ATATAACGAA
    ACAGCATTGA TATGTTTAAC GAATATGGGC GATATCATGG TTTTATCAGT ACCTGAATTA
    AAAAGACAGC TGAATGCCGC AGCAGTGCGA CGGGAAGACA TTAATGGAGT TTCGTCACTT
    TGCTTTACAA ACTCTGGAGA AGCACTGTAT ATGATGTCTT CTTCTGAACT GCAGCGTATT
    GCTTTAGCCA CGTCCAGAGT CGTGCAACCC ACTGGCGTTG TTCCAGTAGA ACCATTAGAA
    AATGAAGAGT CTGTGTTGGA AGAAAATGAT GCAGAGAATA ATAAGGAAAC CTACGCATGT
    GATGAAGTTG TGAATACATA TGAAATTAAA AATCCATCAG GCATTTCAAT ATGCACAAGG
    CCTGCAGAGG AAAACGTTGG AAGAAATAGT GTTCAGCAAG TTAATGGAGT CAACATTTCA
    AATTCACCTA ATCAAGCTAA CGAGACTATC AGCAGCTCTA TTGGCGATAT TACCGTTGAC
    TCGGTGCGCG ACCATTTAAA TATGACGACC ACCACTTTGT GTTCTATTAA TACAGAGGAA
    ACCATTGGTC GCCTATCTGT ACTTAGCACG CAAACCAACA AAGCCAGTAC TACCGTAAAC
    ATGAGTGAAA TTCCAAATAT TAATATTTCT AATTTAGAGG ACTTGGAATC GAAAAGAAAT
    ACGACGGAAA CGAGTACTAG TTCTGTTGTA ATTAAATCTA TAATTACAAA CATTTCTCAT
    GAAAAAACGA ACGGAGACAA CAAAATAGGA ACGCCAAAAA CAGCGCCTGA AGAAAGCCAA
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa13291
    Clone ID Related Accession (Same CDS sequence) NM_078715.4 , NM_164349.3 , NM_001258873.2 , NM_001258872.2 , NM_001258874.2 , NM_001258875.2
    Accession Version NM_164349.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3486bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product lethal (2) giant larvae, isoform A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164349.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTTAAAGT TTATCAGAGG AAAAGGGCAG CAGCCCAGTG CTGACAGACA CCGCCTACAG 
    AAGGACCTTT TTGCTTATCG TAAGACGGCA CAGCATGGCT TTCCTCATAA GCCTTCGGCT
    CTTGCGTATG ATCCAGTTTT GAAACTTATG GCAATAGGGA CGCAAACAGG GGCTTTAAAA
    GTTTTCGGTC AACCCGGAGT TGAATTGTAC GGTCAGCATA CTTTGTTAAA CAATTCAGCA
    TCGGAGCTTA ATGTACAATT ACTTGAATGG GTGTATGGAA CTGGTCGCAT ACTTTCGTTG
    ACGGCAGCGA ATCAATTAAT TCTATGGGAG CCAGTTGGAG CAACGTTGCT GCCAATCAAA
    ACACTACCGT TTGACGGCAA ACTTAAAAAA GTTTCATCGC TGTGCTGTTC TCTCAGTAAG
    GATCTGCTAT GGATTGGAAC AGAAGGTGGA AACATCTATC AACTGGATTT ACATACATTT
    ACCATTAAGG AGCCTGTAAT TTACCATGAC GTTGTGCTAG AGCAGGTGCC ACCAGCCTAC
    AAGCTAAATC CTGGTGCAAT TGAGTCAATC CGCCAACTTC CAAACTCCCC TAGCAAACTT
    CTAGTTGCAT ACAATCGCGG CCTTTGTGTT TTGTGGGATT TTGAAAGCGC ATCTGTCCAG
    CGAGCATACA TAGCCCCTGG ACATGGACAG AGCGTTGGTC TTACAGTGAA CTTCGAAGGA
    TCTGAATTTA CCTGGTACCA CGCTGATGGT TCATACGCCA CTTGGAGCAT AGATAACCCA
    GAACCGCCGT CGAATGTTAA TTATGTGCCT TATGGACCTG ATCCATGCAA AAGCATAAAT
    CGACTGTACA AAGGCAAGCG AAGATCCAAC GATGTAATTG TTTTTTCCGG CGGCATGCCA
    CGGTCAGCAT ATGGTGATCA CAATTGTGTG TCCGTTCACG CCAGCGATGG ACACAAAGTG
    TGTCTTGACT TTACGTCTAA AGTGATTGAC TTTTTTGTGA CCTTTGAAAA TAATAGAGAT
    GTCGCTGAAG TTCTTGTTGT ACTACTTGAA GAGGAACTCT GCGCTTACGA TCTTACTGAC
    CCTAATATTT GTGCTATCAA AGCGCCATAT CTTCACTCTG TCCATGCATC AGCTGTCACT
    TGCAATTACC TTGCTTCTGA AGTCGTACAG TCGGTATATG AAAGTATTTT AAGAGCTGGA
    GATGAACAAG ACATTGACTA TAGCAATATT AGCTGGCCTA TCACTGGCGG TACTCTCCCG
    GATAACTTAG AAGAATCTGT AGAAGAGGAC GCGACTAAGC TTTATGAGAT TTTGTTAACT
    GGTCACGAAG ATGGTTCTGT TAAATTTTGG GACTGCACTG GAGTGTTGCT TAAACCAATT
    TATAATTTTA AAACTAGCAG CATTTTTGGA AGTGAGTCAG ACTTCCGAGA TGACGCAGCT
    GCAGATATGA GTGCGGAACA AGTCGATGAA GGAGAACCGC CATTTCGGAA ATCAGGACTT
    TTTGATCCTT ATTCAGATGA CCCTCGTTTA GCAGTGAAGA AAATAGCATT CTGCCCAAAA
    ACCGGACAAC TTATTGTTGG TGGCACAGCG GGCCAAATAG TTATAGCCGA CTTCATAGAC
    TTACCCGAAA AAGTGTCTTT AAAATACATT TCAATGAATT TGGTCAGCGA TCGTGATGGA
    TTTGTGTGGA AGGGTCACGA TCAGTTAAAC GTGCGATCGA ACTTATTAGA CGGAGAAGCA
    ATTCCTACGA CGGAACGTGG TGTAAATATA TCGGGAGTAC TGCAAGTTTT GCCGCCAGCC
    AGCATAACAT GCATGGCACT CGAAGCAAGC TGGGGCCTAG TATCTGGTGG GACTGCGCAC
    GGCTTAGTTC TCTTTGACTT CAAAAACTTT GTTCCAGTAT TTCATCGCTG CACTTTAAAC
    CCAAATGATC TTACTGGAGC AGGAGAGCAG CTGTCTCGTC GAAAGTCTTT TAAGAAATCA
    TTGAGGGAGT CATTTAGAAA GCTTCGCAAG GGTCGATCGA CCAGGACCAA CCAGAGCAAT
    CAAGTACCAA CAACGCTGGA AGCAAGACCC GTCGAGAGGC AAATAGAGGC TCGTTGTGCA
    GATGACGGGC TAGGATCCAT GGTGCGATGT TTACTATTTG CCAAAACTTA TGTTACTAAT
    GTCAACATAA CGTCGCCAAC TTTGTGGTCA GCAACAAATG CCAGTACAGT CTCGGTTTTC
    CTTCTGCATT TGCCACCAGC GCAGACCGCG GCAACTGCCG TCCCGTCGGC AAGTGGCAAT
    GCACCACCAC ACATGCCCCG CCGAATTTCT GCGCAGCTTG CTAAAGAAAT ACAATTAAAA
    CATCGTGCTC CTGTGGTGGG TATTTCTATT TTTGATCAGG CGGGTAGCCC TGTCGATCAG
    CTGAACGCCG GTGAAAACGG GAGTCCACCG CATCGTGTAC TTATTGCTTC CGAGGAACAG
    TTCAAGGTGT TTTCACTTCC GCAACTAAAG CCGATTAACA AATATAAGCT TACCGCTAAC
    GAAGGTGCTC GGATTCGCCG CATCCATTTT GGTTCGTTTA GTTGTCGCAT ATCCCCGGAA
    ACACTGCAGA GTATGCACGG TTGTAGCCCA ACTAAGTCCA CGCGTTCACA TGGCGATGGA
    GAGGCGGATC CTAATATCAG TGGAAGCTTG GCTGTAAGTC GTGGAGATGT ATATAACGAA
    ACAGCATTGA TATGTTTAAC GAATATGGGC GATATCATGG TTTTATCAGT ACCTGAATTA
    AAAAGACAGC TGAATGCCGC AGCAGTGCGA CGGGAAGACA TTAATGGAGT TTCGTCACTT
    TGCTTTACAA ACTCTGGAGA AGCACTGTAT ATGATGTCTT CTTCTGAACT GCAGCGTATT
    GCTTTAGCCA CGTCCAGAGT CGTGCAACCC ACTGGCGTTG TTCCAGTAGA ACCATTAGAA
    AATGAAGAGT CTGTGTTGGA AGAAAATGAT GCAGAGAATA ATAAGGAAAC CTACGCATGT
    GATGAAGTTG TGAATACATA TGAAATTAAA AATCCATCAG GCATTTCAAT ATGCACAAGG
    CCTGCAGAGG AAAACGTTGG AAGAAATAGT GTTCAGCAAG TTAATGGAGT CAACATTTCA
    AATTCACCTA ATCAAGCTAA CGAGACTATC AGCAGCTCTA TTGGCGATAT TACCGTTGAC
    TCGGTGCGCG ACCATTTAAA TATGACGACC ACCACTTTGT GTTCTATTAA TACAGAGGAA
    ACCATTGGTC GCCTATCTGT ACTTAGCACG CAAACCAACA AAGCCAGTAC TACCGTAAAC
    ATGAGTGAAA TTCCAAATAT TAATATTTCT AATTTAGAGG ACTTGGAATC GAAAAGAAAT
    ACGACGGAAA CGAGTACTAG TTCTGTTGTA ATTAAATCTA TAATTACAAA CATTTCTCAT
    GAAAAAACGA ACGGAGACAA CAAAATAGGA ACGCCAAAAA CAGCGCCTGA AGAAAGCCAA
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_722584.1
    CDS530..4015
    Translation

    Target ORF information:

    RefSeq Version NM_164349.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster lethal (2) giant larvae (l(2)gl), transcript variant A, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_164349.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTTAAAGT TTATCAGAGG AAAAGGGCAG CAGCCCAGTG CTGACAGACA CCGCCTACAG 
    AAGGACCTTT TTGCTTATCG TAAGACGGCA CAGCATGGCT TTCCTCATAA GCCTTCGGCT
    CTTGCGTATG ATCCAGTTTT GAAACTTATG GCAATAGGGA CGCAAACAGG GGCTTTAAAA
    GTTTTCGGTC AACCCGGAGT TGAATTGTAC GGTCAGCATA CTTTGTTAAA CAATTCAGCA
    TCGGAGCTTA ATGTACAATT ACTTGAATGG GTGTATGGAA CTGGTCGCAT ACTTTCGTTG
    ACGGCAGCGA ATCAATTAAT TCTATGGGAG CCAGTTGGAG CAACGTTGCT GCCAATCAAA
    ACACTACCGT TTGACGGCAA ACTTAAAAAA GTTTCATCGC TGTGCTGTTC TCTCAGTAAG
    GATCTGCTAT GGATTGGAAC AGAAGGTGGA AACATCTATC AACTGGATTT ACATACATTT
    ACCATTAAGG AGCCTGTAAT TTACCATGAC GTTGTGCTAG AGCAGGTGCC ACCAGCCTAC
    AAGCTAAATC CTGGTGCAAT TGAGTCAATC CGCCAACTTC CAAACTCCCC TAGCAAACTT
    CTAGTTGCAT ACAATCGCGG CCTTTGTGTT TTGTGGGATT TTGAAAGCGC ATCTGTCCAG
    CGAGCATACA TAGCCCCTGG ACATGGACAG AGCGTTGGTC TTACAGTGAA CTTCGAAGGA
    TCTGAATTTA CCTGGTACCA CGCTGATGGT TCATACGCCA CTTGGAGCAT AGATAACCCA
    GAACCGCCGT CGAATGTTAA TTATGTGCCT TATGGACCTG ATCCATGCAA AAGCATAAAT
    CGACTGTACA AAGGCAAGCG AAGATCCAAC GATGTAATTG TTTTTTCCGG CGGCATGCCA
    CGGTCAGCAT ATGGTGATCA CAATTGTGTG TCCGTTCACG CCAGCGATGG ACACAAAGTG
    TGTCTTGACT TTACGTCTAA AGTGATTGAC TTTTTTGTGA CCTTTGAAAA TAATAGAGAT
    GTCGCTGAAG TTCTTGTTGT ACTACTTGAA GAGGAACTCT GCGCTTACGA TCTTACTGAC
    CCTAATATTT GTGCTATCAA AGCGCCATAT CTTCACTCTG TCCATGCATC AGCTGTCACT
    TGCAATTACC TTGCTTCTGA AGTCGTACAG TCGGTATATG AAAGTATTTT AAGAGCTGGA
    GATGAACAAG ACATTGACTA TAGCAATATT AGCTGGCCTA TCACTGGCGG TACTCTCCCG
    GATAACTTAG AAGAATCTGT AGAAGAGGAC GCGACTAAGC TTTATGAGAT TTTGTTAACT
    GGTCACGAAG ATGGTTCTGT TAAATTTTGG GACTGCACTG GAGTGTTGCT TAAACCAATT
    TATAATTTTA AAACTAGCAG CATTTTTGGA AGTGAGTCAG ACTTCCGAGA TGACGCAGCT
    GCAGATATGA GTGCGGAACA AGTCGATGAA GGAGAACCGC CATTTCGGAA ATCAGGACTT
    TTTGATCCTT ATTCAGATGA CCCTCGTTTA GCAGTGAAGA AAATAGCATT CTGCCCAAAA
    ACCGGACAAC TTATTGTTGG TGGCACAGCG GGCCAAATAG TTATAGCCGA CTTCATAGAC
    TTACCCGAAA AAGTGTCTTT AAAATACATT TCAATGAATT TGGTCAGCGA TCGTGATGGA
    TTTGTGTGGA AGGGTCACGA TCAGTTAAAC GTGCGATCGA ACTTATTAGA CGGAGAAGCA
    ATTCCTACGA CGGAACGTGG TGTAAATATA TCGGGAGTAC TGCAAGTTTT GCCGCCAGCC
    AGCATAACAT GCATGGCACT CGAAGCAAGC TGGGGCCTAG TATCTGGTGG GACTGCGCAC
    GGCTTAGTTC TCTTTGACTT CAAAAACTTT GTTCCAGTAT TTCATCGCTG CACTTTAAAC
    CCAAATGATC TTACTGGAGC AGGAGAGCAG CTGTCTCGTC GAAAGTCTTT TAAGAAATCA
    TTGAGGGAGT CATTTAGAAA GCTTCGCAAG GGTCGATCGA CCAGGACCAA CCAGAGCAAT
    CAAGTACCAA CAACGCTGGA AGCAAGACCC GTCGAGAGGC AAATAGAGGC TCGTTGTGCA
    GATGACGGGC TAGGATCCAT GGTGCGATGT TTACTATTTG CCAAAACTTA TGTTACTAAT
    GTCAACATAA CGTCGCCAAC TTTGTGGTCA GCAACAAATG CCAGTACAGT CTCGGTTTTC
    CTTCTGCATT TGCCACCAGC GCAGACCGCG GCAACTGCCG TCCCGTCGGC AAGTGGCAAT
    GCACCACCAC ACATGCCCCG CCGAATTTCT GCGCAGCTTG CTAAAGAAAT ACAATTAAAA
    CATCGTGCTC CTGTGGTGGG TATTTCTATT TTTGATCAGG CGGGTAGCCC TGTCGATCAG
    CTGAACGCCG GTGAAAACGG GAGTCCACCG CATCGTGTAC TTATTGCTTC CGAGGAACAG
    TTCAAGGTGT TTTCACTTCC GCAACTAAAG CCGATTAACA AATATAAGCT TACCGCTAAC
    GAAGGTGCTC GGATTCGCCG CATCCATTTT GGTTCGTTTA GTTGTCGCAT ATCCCCGGAA
    ACACTGCAGA GTATGCACGG TTGTAGCCCA ACTAAGTCCA CGCGTTCACA TGGCGATGGA
    GAGGCGGATC CTAATATCAG TGGAAGCTTG GCTGTAAGTC GTGGAGATGT ATATAACGAA
    ACAGCATTGA TATGTTTAAC GAATATGGGC GATATCATGG TTTTATCAGT ACCTGAATTA
    AAAAGACAGC TGAATGCCGC AGCAGTGCGA CGGGAAGACA TTAATGGAGT TTCGTCACTT
    TGCTTTACAA ACTCTGGAGA AGCACTGTAT ATGATGTCTT CTTCTGAACT GCAGCGTATT
    GCTTTAGCCA CGTCCAGAGT CGTGCAACCC ACTGGCGTTG TTCCAGTAGA ACCATTAGAA
    AATGAAGAGT CTGTGTTGGA AGAAAATGAT GCAGAGAATA ATAAGGAAAC CTACGCATGT
    GATGAAGTTG TGAATACATA TGAAATTAAA AATCCATCAG GCATTTCAAT ATGCACAAGG
    CCTGCAGAGG AAAACGTTGG AAGAAATAGT GTTCAGCAAG TTAATGGAGT CAACATTTCA
    AATTCACCTA ATCAAGCTAA CGAGACTATC AGCAGCTCTA TTGGCGATAT TACCGTTGAC
    TCGGTGCGCG ACCATTTAAA TATGACGACC ACCACTTTGT GTTCTATTAA TACAGAGGAA
    ACCATTGGTC GCCTATCTGT ACTTAGCACG CAAACCAACA AAGCCAGTAC TACCGTAAAC
    ATGAGTGAAA TTCCAAATAT TAATATTTCT AATTTAGAGG ACTTGGAATC GAAAAGAAAT
    ACGACGGAAA CGAGTACTAG TTCTGTTGTA ATTAAATCTA TAATTACAAA CATTTCTCAT
    GAAAAAACGA ACGGAGACAA CAAAATAGGA ACGCCAAAAA CAGCGCCTGA AGAAAGCCAA
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa13291
    Clone ID Related Accession (Same CDS sequence) NM_078715.4 , NM_164349.3 , NM_001258873.2 , NM_001258872.2 , NM_001258874.2 , NM_001258875.2
    Accession Version NM_001258873.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3486bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product lethal (2) giant larvae, isoform H
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001258873.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTTAAAGT TTATCAGAGG AAAAGGGCAG CAGCCCAGTG CTGACAGACA CCGCCTACAG 
    AAGGACCTTT TTGCTTATCG TAAGACGGCA CAGCATGGCT TTCCTCATAA GCCTTCGGCT
    CTTGCGTATG ATCCAGTTTT GAAACTTATG GCAATAGGGA CGCAAACAGG GGCTTTAAAA
    GTTTTCGGTC AACCCGGAGT TGAATTGTAC GGTCAGCATA CTTTGTTAAA CAATTCAGCA
    TCGGAGCTTA ATGTACAATT ACTTGAATGG GTGTATGGAA CTGGTCGCAT ACTTTCGTTG
    ACGGCAGCGA ATCAATTAAT TCTATGGGAG CCAGTTGGAG CAACGTTGCT GCCAATCAAA
    ACACTACCGT TTGACGGCAA ACTTAAAAAA GTTTCATCGC TGTGCTGTTC TCTCAGTAAG
    GATCTGCTAT GGATTGGAAC AGAAGGTGGA AACATCTATC AACTGGATTT ACATACATTT
    ACCATTAAGG AGCCTGTAAT TTACCATGAC GTTGTGCTAG AGCAGGTGCC ACCAGCCTAC
    AAGCTAAATC CTGGTGCAAT TGAGTCAATC CGCCAACTTC CAAACTCCCC TAGCAAACTT
    CTAGTTGCAT ACAATCGCGG CCTTTGTGTT TTGTGGGATT TTGAAAGCGC ATCTGTCCAG
    CGAGCATACA TAGCCCCTGG ACATGGACAG AGCGTTGGTC TTACAGTGAA CTTCGAAGGA
    TCTGAATTTA CCTGGTACCA CGCTGATGGT TCATACGCCA CTTGGAGCAT AGATAACCCA
    GAACCGCCGT CGAATGTTAA TTATGTGCCT TATGGACCTG ATCCATGCAA AAGCATAAAT
    CGACTGTACA AAGGCAAGCG AAGATCCAAC GATGTAATTG TTTTTTCCGG CGGCATGCCA
    CGGTCAGCAT ATGGTGATCA CAATTGTGTG TCCGTTCACG CCAGCGATGG ACACAAAGTG
    TGTCTTGACT TTACGTCTAA AGTGATTGAC TTTTTTGTGA CCTTTGAAAA TAATAGAGAT
    GTCGCTGAAG TTCTTGTTGT ACTACTTGAA GAGGAACTCT GCGCTTACGA TCTTACTGAC
    CCTAATATTT GTGCTATCAA AGCGCCATAT CTTCACTCTG TCCATGCATC AGCTGTCACT
    TGCAATTACC TTGCTTCTGA AGTCGTACAG TCGGTATATG AAAGTATTTT AAGAGCTGGA
    GATGAACAAG ACATTGACTA TAGCAATATT AGCTGGCCTA TCACTGGCGG TACTCTCCCG
    GATAACTTAG AAGAATCTGT AGAAGAGGAC GCGACTAAGC TTTATGAGAT TTTGTTAACT
    GGTCACGAAG ATGGTTCTGT TAAATTTTGG GACTGCACTG GAGTGTTGCT TAAACCAATT
    TATAATTTTA AAACTAGCAG CATTTTTGGA AGTGAGTCAG ACTTCCGAGA TGACGCAGCT
    GCAGATATGA GTGCGGAACA AGTCGATGAA GGAGAACCGC CATTTCGGAA ATCAGGACTT
    TTTGATCCTT ATTCAGATGA CCCTCGTTTA GCAGTGAAGA AAATAGCATT CTGCCCAAAA
    ACCGGACAAC TTATTGTTGG TGGCACAGCG GGCCAAATAG TTATAGCCGA CTTCATAGAC
    TTACCCGAAA AAGTGTCTTT AAAATACATT TCAATGAATT TGGTCAGCGA TCGTGATGGA
    TTTGTGTGGA AGGGTCACGA TCAGTTAAAC GTGCGATCGA ACTTATTAGA CGGAGAAGCA
    ATTCCTACGA CGGAACGTGG TGTAAATATA TCGGGAGTAC TGCAAGTTTT GCCGCCAGCC
    AGCATAACAT GCATGGCACT CGAAGCAAGC TGGGGCCTAG TATCTGGTGG GACTGCGCAC
    GGCTTAGTTC TCTTTGACTT CAAAAACTTT GTTCCAGTAT TTCATCGCTG CACTTTAAAC
    CCAAATGATC TTACTGGAGC AGGAGAGCAG CTGTCTCGTC GAAAGTCTTT TAAGAAATCA
    TTGAGGGAGT CATTTAGAAA GCTTCGCAAG GGTCGATCGA CCAGGACCAA CCAGAGCAAT
    CAAGTACCAA CAACGCTGGA AGCAAGACCC GTCGAGAGGC AAATAGAGGC TCGTTGTGCA
    GATGACGGGC TAGGATCCAT GGTGCGATGT TTACTATTTG CCAAAACTTA TGTTACTAAT
    GTCAACATAA CGTCGCCAAC TTTGTGGTCA GCAACAAATG CCAGTACAGT CTCGGTTTTC
    CTTCTGCATT TGCCACCAGC GCAGACCGCG GCAACTGCCG TCCCGTCGGC AAGTGGCAAT
    GCACCACCAC ACATGCCCCG CCGAATTTCT GCGCAGCTTG CTAAAGAAAT ACAATTAAAA
    CATCGTGCTC CTGTGGTGGG TATTTCTATT TTTGATCAGG CGGGTAGCCC TGTCGATCAG
    CTGAACGCCG GTGAAAACGG GAGTCCACCG CATCGTGTAC TTATTGCTTC CGAGGAACAG
    TTCAAGGTGT TTTCACTTCC GCAACTAAAG CCGATTAACA AATATAAGCT TACCGCTAAC
    GAAGGTGCTC GGATTCGCCG CATCCATTTT GGTTCGTTTA GTTGTCGCAT ATCCCCGGAA
    ACACTGCAGA GTATGCACGG TTGTAGCCCA ACTAAGTCCA CGCGTTCACA TGGCGATGGA
    GAGGCGGATC CTAATATCAG TGGAAGCTTG GCTGTAAGTC GTGGAGATGT ATATAACGAA
    ACAGCATTGA TATGTTTAAC GAATATGGGC GATATCATGG TTTTATCAGT ACCTGAATTA
    AAAAGACAGC TGAATGCCGC AGCAGTGCGA CGGGAAGACA TTAATGGAGT TTCGTCACTT
    TGCTTTACAA ACTCTGGAGA AGCACTGTAT ATGATGTCTT CTTCTGAACT GCAGCGTATT
    GCTTTAGCCA CGTCCAGAGT CGTGCAACCC ACTGGCGTTG TTCCAGTAGA ACCATTAGAA
    AATGAAGAGT CTGTGTTGGA AGAAAATGAT GCAGAGAATA ATAAGGAAAC CTACGCATGT
    GATGAAGTTG TGAATACATA TGAAATTAAA AATCCATCAG GCATTTCAAT ATGCACAAGG
    CCTGCAGAGG AAAACGTTGG AAGAAATAGT GTTCAGCAAG TTAATGGAGT CAACATTTCA
    AATTCACCTA ATCAAGCTAA CGAGACTATC AGCAGCTCTA TTGGCGATAT TACCGTTGAC
    TCGGTGCGCG ACCATTTAAA TATGACGACC ACCACTTTGT GTTCTATTAA TACAGAGGAA
    ACCATTGGTC GCCTATCTGT ACTTAGCACG CAAACCAACA AAGCCAGTAC TACCGTAAAC
    ATGAGTGAAA TTCCAAATAT TAATATTTCT AATTTAGAGG ACTTGGAATC GAAAAGAAAT
    ACGACGGAAA CGAGTACTAG TTCTGTTGTA ATTAAATCTA TAATTACAAA CATTTCTCAT
    GAAAAAACGA ACGGAGACAA CAAAATAGGA ACGCCAAAAA CAGCGCCTGA AGAAAGCCAA
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001245802.1
    CDS318..3803
    Translation

    Target ORF information:

    RefSeq Version NM_001258873.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster lethal (2) giant larvae (l(2)gl), transcript variant H, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001258873.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTTAAAGT TTATCAGAGG AAAAGGGCAG CAGCCCAGTG CTGACAGACA CCGCCTACAG 
    AAGGACCTTT TTGCTTATCG TAAGACGGCA CAGCATGGCT TTCCTCATAA GCCTTCGGCT
    CTTGCGTATG ATCCAGTTTT GAAACTTATG GCAATAGGGA CGCAAACAGG GGCTTTAAAA
    GTTTTCGGTC AACCCGGAGT TGAATTGTAC GGTCAGCATA CTTTGTTAAA CAATTCAGCA
    TCGGAGCTTA ATGTACAATT ACTTGAATGG GTGTATGGAA CTGGTCGCAT ACTTTCGTTG
    ACGGCAGCGA ATCAATTAAT TCTATGGGAG CCAGTTGGAG CAACGTTGCT GCCAATCAAA
    ACACTACCGT TTGACGGCAA ACTTAAAAAA GTTTCATCGC TGTGCTGTTC TCTCAGTAAG
    GATCTGCTAT GGATTGGAAC AGAAGGTGGA AACATCTATC AACTGGATTT ACATACATTT
    ACCATTAAGG AGCCTGTAAT TTACCATGAC GTTGTGCTAG AGCAGGTGCC ACCAGCCTAC
    AAGCTAAATC CTGGTGCAAT TGAGTCAATC CGCCAACTTC CAAACTCCCC TAGCAAACTT
    CTAGTTGCAT ACAATCGCGG CCTTTGTGTT TTGTGGGATT TTGAAAGCGC ATCTGTCCAG
    CGAGCATACA TAGCCCCTGG ACATGGACAG AGCGTTGGTC TTACAGTGAA CTTCGAAGGA
    TCTGAATTTA CCTGGTACCA CGCTGATGGT TCATACGCCA CTTGGAGCAT AGATAACCCA
    GAACCGCCGT CGAATGTTAA TTATGTGCCT TATGGACCTG ATCCATGCAA AAGCATAAAT
    CGACTGTACA AAGGCAAGCG AAGATCCAAC GATGTAATTG TTTTTTCCGG CGGCATGCCA
    CGGTCAGCAT ATGGTGATCA CAATTGTGTG TCCGTTCACG CCAGCGATGG ACACAAAGTG
    TGTCTTGACT TTACGTCTAA AGTGATTGAC TTTTTTGTGA CCTTTGAAAA TAATAGAGAT
    GTCGCTGAAG TTCTTGTTGT ACTACTTGAA GAGGAACTCT GCGCTTACGA TCTTACTGAC
    CCTAATATTT GTGCTATCAA AGCGCCATAT CTTCACTCTG TCCATGCATC AGCTGTCACT
    TGCAATTACC TTGCTTCTGA AGTCGTACAG TCGGTATATG AAAGTATTTT AAGAGCTGGA
    GATGAACAAG ACATTGACTA TAGCAATATT AGCTGGCCTA TCACTGGCGG TACTCTCCCG
    GATAACTTAG AAGAATCTGT AGAAGAGGAC GCGACTAAGC TTTATGAGAT TTTGTTAACT
    GGTCACGAAG ATGGTTCTGT TAAATTTTGG GACTGCACTG GAGTGTTGCT TAAACCAATT
    TATAATTTTA AAACTAGCAG CATTTTTGGA AGTGAGTCAG ACTTCCGAGA TGACGCAGCT
    GCAGATATGA GTGCGGAACA AGTCGATGAA GGAGAACCGC CATTTCGGAA ATCAGGACTT
    TTTGATCCTT ATTCAGATGA CCCTCGTTTA GCAGTGAAGA AAATAGCATT CTGCCCAAAA
    ACCGGACAAC TTATTGTTGG TGGCACAGCG GGCCAAATAG TTATAGCCGA CTTCATAGAC
    TTACCCGAAA AAGTGTCTTT AAAATACATT TCAATGAATT TGGTCAGCGA TCGTGATGGA
    TTTGTGTGGA AGGGTCACGA TCAGTTAAAC GTGCGATCGA ACTTATTAGA CGGAGAAGCA
    ATTCCTACGA CGGAACGTGG TGTAAATATA TCGGGAGTAC TGCAAGTTTT GCCGCCAGCC
    AGCATAACAT GCATGGCACT CGAAGCAAGC TGGGGCCTAG TATCTGGTGG GACTGCGCAC
    GGCTTAGTTC TCTTTGACTT CAAAAACTTT GTTCCAGTAT TTCATCGCTG CACTTTAAAC
    CCAAATGATC TTACTGGAGC AGGAGAGCAG CTGTCTCGTC GAAAGTCTTT TAAGAAATCA
    TTGAGGGAGT CATTTAGAAA GCTTCGCAAG GGTCGATCGA CCAGGACCAA CCAGAGCAAT
    CAAGTACCAA CAACGCTGGA AGCAAGACCC GTCGAGAGGC AAATAGAGGC TCGTTGTGCA
    GATGACGGGC TAGGATCCAT GGTGCGATGT TTACTATTTG CCAAAACTTA TGTTACTAAT
    GTCAACATAA CGTCGCCAAC TTTGTGGTCA GCAACAAATG CCAGTACAGT CTCGGTTTTC
    CTTCTGCATT TGCCACCAGC GCAGACCGCG GCAACTGCCG TCCCGTCGGC AAGTGGCAAT
    GCACCACCAC ACATGCCCCG CCGAATTTCT GCGCAGCTTG CTAAAGAAAT ACAATTAAAA
    CATCGTGCTC CTGTGGTGGG TATTTCTATT TTTGATCAGG CGGGTAGCCC TGTCGATCAG
    CTGAACGCCG GTGAAAACGG GAGTCCACCG CATCGTGTAC TTATTGCTTC CGAGGAACAG
    TTCAAGGTGT TTTCACTTCC GCAACTAAAG CCGATTAACA AATATAAGCT TACCGCTAAC
    GAAGGTGCTC GGATTCGCCG CATCCATTTT GGTTCGTTTA GTTGTCGCAT ATCCCCGGAA
    ACACTGCAGA GTATGCACGG TTGTAGCCCA ACTAAGTCCA CGCGTTCACA TGGCGATGGA
    GAGGCGGATC CTAATATCAG TGGAAGCTTG GCTGTAAGTC GTGGAGATGT ATATAACGAA
    ACAGCATTGA TATGTTTAAC GAATATGGGC GATATCATGG TTTTATCAGT ACCTGAATTA
    AAAAGACAGC TGAATGCCGC AGCAGTGCGA CGGGAAGACA TTAATGGAGT TTCGTCACTT
    TGCTTTACAA ACTCTGGAGA AGCACTGTAT ATGATGTCTT CTTCTGAACT GCAGCGTATT
    GCTTTAGCCA CGTCCAGAGT CGTGCAACCC ACTGGCGTTG TTCCAGTAGA ACCATTAGAA
    AATGAAGAGT CTGTGTTGGA AGAAAATGAT GCAGAGAATA ATAAGGAAAC CTACGCATGT
    GATGAAGTTG TGAATACATA TGAAATTAAA AATCCATCAG GCATTTCAAT ATGCACAAGG
    CCTGCAGAGG AAAACGTTGG AAGAAATAGT GTTCAGCAAG TTAATGGAGT CAACATTTCA
    AATTCACCTA ATCAAGCTAA CGAGACTATC AGCAGCTCTA TTGGCGATAT TACCGTTGAC
    TCGGTGCGCG ACCATTTAAA TATGACGACC ACCACTTTGT GTTCTATTAA TACAGAGGAA
    ACCATTGGTC GCCTATCTGT ACTTAGCACG CAAACCAACA AAGCCAGTAC TACCGTAAAC
    ATGAGTGAAA TTCCAAATAT TAATATTTCT AATTTAGAGG ACTTGGAATC GAAAAGAAAT
    ACGACGGAAA CGAGTACTAG TTCTGTTGTA ATTAAATCTA TAATTACAAA CATTTCTCAT
    GAAAAAACGA ACGGAGACAA CAAAATAGGA ACGCCAAAAA CAGCGCCTGA AGAAAGCCAA
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa13291
    Clone ID Related Accession (Same CDS sequence) NM_078715.4 , NM_164349.3 , NM_001258873.2 , NM_001258872.2 , NM_001258874.2 , NM_001258875.2
    Accession Version NM_001258872.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3486bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product lethal (2) giant larvae, isoform G
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001258872.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTTAAAGT TTATCAGAGG AAAAGGGCAG CAGCCCAGTG CTGACAGACA CCGCCTACAG 
    AAGGACCTTT TTGCTTATCG TAAGACGGCA CAGCATGGCT TTCCTCATAA GCCTTCGGCT
    CTTGCGTATG ATCCAGTTTT GAAACTTATG GCAATAGGGA CGCAAACAGG GGCTTTAAAA
    GTTTTCGGTC AACCCGGAGT TGAATTGTAC GGTCAGCATA CTTTGTTAAA CAATTCAGCA
    TCGGAGCTTA ATGTACAATT ACTTGAATGG GTGTATGGAA CTGGTCGCAT ACTTTCGTTG
    ACGGCAGCGA ATCAATTAAT TCTATGGGAG CCAGTTGGAG CAACGTTGCT GCCAATCAAA
    ACACTACCGT TTGACGGCAA ACTTAAAAAA GTTTCATCGC TGTGCTGTTC TCTCAGTAAG
    GATCTGCTAT GGATTGGAAC AGAAGGTGGA AACATCTATC AACTGGATTT ACATACATTT
    ACCATTAAGG AGCCTGTAAT TTACCATGAC GTTGTGCTAG AGCAGGTGCC ACCAGCCTAC
    AAGCTAAATC CTGGTGCAAT TGAGTCAATC CGCCAACTTC CAAACTCCCC TAGCAAACTT
    CTAGTTGCAT ACAATCGCGG CCTTTGTGTT TTGTGGGATT TTGAAAGCGC ATCTGTCCAG
    CGAGCATACA TAGCCCCTGG ACATGGACAG AGCGTTGGTC TTACAGTGAA CTTCGAAGGA
    TCTGAATTTA CCTGGTACCA CGCTGATGGT TCATACGCCA CTTGGAGCAT AGATAACCCA
    GAACCGCCGT CGAATGTTAA TTATGTGCCT TATGGACCTG ATCCATGCAA AAGCATAAAT
    CGACTGTACA AAGGCAAGCG AAGATCCAAC GATGTAATTG TTTTTTCCGG CGGCATGCCA
    CGGTCAGCAT ATGGTGATCA CAATTGTGTG TCCGTTCACG CCAGCGATGG ACACAAAGTG
    TGTCTTGACT TTACGTCTAA AGTGATTGAC TTTTTTGTGA CCTTTGAAAA TAATAGAGAT
    GTCGCTGAAG TTCTTGTTGT ACTACTTGAA GAGGAACTCT GCGCTTACGA TCTTACTGAC
    CCTAATATTT GTGCTATCAA AGCGCCATAT CTTCACTCTG TCCATGCATC AGCTGTCACT
    TGCAATTACC TTGCTTCTGA AGTCGTACAG TCGGTATATG AAAGTATTTT AAGAGCTGGA
    GATGAACAAG ACATTGACTA TAGCAATATT AGCTGGCCTA TCACTGGCGG TACTCTCCCG
    GATAACTTAG AAGAATCTGT AGAAGAGGAC GCGACTAAGC TTTATGAGAT TTTGTTAACT
    GGTCACGAAG ATGGTTCTGT TAAATTTTGG GACTGCACTG GAGTGTTGCT TAAACCAATT
    TATAATTTTA AAACTAGCAG CATTTTTGGA AGTGAGTCAG ACTTCCGAGA TGACGCAGCT
    GCAGATATGA GTGCGGAACA AGTCGATGAA GGAGAACCGC CATTTCGGAA ATCAGGACTT
    TTTGATCCTT ATTCAGATGA CCCTCGTTTA GCAGTGAAGA AAATAGCATT CTGCCCAAAA
    ACCGGACAAC TTATTGTTGG TGGCACAGCG GGCCAAATAG TTATAGCCGA CTTCATAGAC
    TTACCCGAAA AAGTGTCTTT AAAATACATT TCAATGAATT TGGTCAGCGA TCGTGATGGA
    TTTGTGTGGA AGGGTCACGA TCAGTTAAAC GTGCGATCGA ACTTATTAGA CGGAGAAGCA
    ATTCCTACGA CGGAACGTGG TGTAAATATA TCGGGAGTAC TGCAAGTTTT GCCGCCAGCC
    AGCATAACAT GCATGGCACT CGAAGCAAGC TGGGGCCTAG TATCTGGTGG GACTGCGCAC
    GGCTTAGTTC TCTTTGACTT CAAAAACTTT GTTCCAGTAT TTCATCGCTG CACTTTAAAC
    CCAAATGATC TTACTGGAGC AGGAGAGCAG CTGTCTCGTC GAAAGTCTTT TAAGAAATCA
    TTGAGGGAGT CATTTAGAAA GCTTCGCAAG GGTCGATCGA CCAGGACCAA CCAGAGCAAT
    CAAGTACCAA CAACGCTGGA AGCAAGACCC GTCGAGAGGC AAATAGAGGC TCGTTGTGCA
    GATGACGGGC TAGGATCCAT GGTGCGATGT TTACTATTTG CCAAAACTTA TGTTACTAAT
    GTCAACATAA CGTCGCCAAC TTTGTGGTCA GCAACAAATG CCAGTACAGT CTCGGTTTTC
    CTTCTGCATT TGCCACCAGC GCAGACCGCG GCAACTGCCG TCCCGTCGGC AAGTGGCAAT
    GCACCACCAC ACATGCCCCG CCGAATTTCT GCGCAGCTTG CTAAAGAAAT ACAATTAAAA
    CATCGTGCTC CTGTGGTGGG TATTTCTATT TTTGATCAGG CGGGTAGCCC TGTCGATCAG
    CTGAACGCCG GTGAAAACGG GAGTCCACCG CATCGTGTAC TTATTGCTTC CGAGGAACAG
    TTCAAGGTGT TTTCACTTCC GCAACTAAAG CCGATTAACA AATATAAGCT TACCGCTAAC
    GAAGGTGCTC GGATTCGCCG CATCCATTTT GGTTCGTTTA GTTGTCGCAT ATCCCCGGAA
    ACACTGCAGA GTATGCACGG TTGTAGCCCA ACTAAGTCCA CGCGTTCACA TGGCGATGGA
    GAGGCGGATC CTAATATCAG TGGAAGCTTG GCTGTAAGTC GTGGAGATGT ATATAACGAA
    ACAGCATTGA TATGTTTAAC GAATATGGGC GATATCATGG TTTTATCAGT ACCTGAATTA
    AAAAGACAGC TGAATGCCGC AGCAGTGCGA CGGGAAGACA TTAATGGAGT TTCGTCACTT
    TGCTTTACAA ACTCTGGAGA AGCACTGTAT ATGATGTCTT CTTCTGAACT GCAGCGTATT
    GCTTTAGCCA CGTCCAGAGT CGTGCAACCC ACTGGCGTTG TTCCAGTAGA ACCATTAGAA
    AATGAAGAGT CTGTGTTGGA AGAAAATGAT GCAGAGAATA ATAAGGAAAC CTACGCATGT
    GATGAAGTTG TGAATACATA TGAAATTAAA AATCCATCAG GCATTTCAAT ATGCACAAGG
    CCTGCAGAGG AAAACGTTGG AAGAAATAGT GTTCAGCAAG TTAATGGAGT CAACATTTCA
    AATTCACCTA ATCAAGCTAA CGAGACTATC AGCAGCTCTA TTGGCGATAT TACCGTTGAC
    TCGGTGCGCG ACCATTTAAA TATGACGACC ACCACTTTGT GTTCTATTAA TACAGAGGAA
    ACCATTGGTC GCCTATCTGT ACTTAGCACG CAAACCAACA AAGCCAGTAC TACCGTAAAC
    ATGAGTGAAA TTCCAAATAT TAATATTTCT AATTTAGAGG ACTTGGAATC GAAAAGAAAT
    ACGACGGAAA CGAGTACTAG TTCTGTTGTA ATTAAATCTA TAATTACAAA CATTTCTCAT
    GAAAAAACGA ACGGAGACAA CAAAATAGGA ACGCCAAAAA CAGCGCCTGA AGAAAGCCAA
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001245801.1
    CDS454..3939
    Translation

    Target ORF information:

    RefSeq Version NM_001258872.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster lethal (2) giant larvae (l(2)gl), transcript variant G, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001258872.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTTAAAGT TTATCAGAGG AAAAGGGCAG CAGCCCAGTG CTGACAGACA CCGCCTACAG 
    AAGGACCTTT TTGCTTATCG TAAGACGGCA CAGCATGGCT TTCCTCATAA GCCTTCGGCT
    CTTGCGTATG ATCCAGTTTT GAAACTTATG GCAATAGGGA CGCAAACAGG GGCTTTAAAA
    GTTTTCGGTC AACCCGGAGT TGAATTGTAC GGTCAGCATA CTTTGTTAAA CAATTCAGCA
    TCGGAGCTTA ATGTACAATT ACTTGAATGG GTGTATGGAA CTGGTCGCAT ACTTTCGTTG
    ACGGCAGCGA ATCAATTAAT TCTATGGGAG CCAGTTGGAG CAACGTTGCT GCCAATCAAA
    ACACTACCGT TTGACGGCAA ACTTAAAAAA GTTTCATCGC TGTGCTGTTC TCTCAGTAAG
    GATCTGCTAT GGATTGGAAC AGAAGGTGGA AACATCTATC AACTGGATTT ACATACATTT
    ACCATTAAGG AGCCTGTAAT TTACCATGAC GTTGTGCTAG AGCAGGTGCC ACCAGCCTAC
    AAGCTAAATC CTGGTGCAAT TGAGTCAATC CGCCAACTTC CAAACTCCCC TAGCAAACTT
    CTAGTTGCAT ACAATCGCGG CCTTTGTGTT TTGTGGGATT TTGAAAGCGC ATCTGTCCAG
    CGAGCATACA TAGCCCCTGG ACATGGACAG AGCGTTGGTC TTACAGTGAA CTTCGAAGGA
    TCTGAATTTA CCTGGTACCA CGCTGATGGT TCATACGCCA CTTGGAGCAT AGATAACCCA
    GAACCGCCGT CGAATGTTAA TTATGTGCCT TATGGACCTG ATCCATGCAA AAGCATAAAT
    CGACTGTACA AAGGCAAGCG AAGATCCAAC GATGTAATTG TTTTTTCCGG CGGCATGCCA
    CGGTCAGCAT ATGGTGATCA CAATTGTGTG TCCGTTCACG CCAGCGATGG ACACAAAGTG
    TGTCTTGACT TTACGTCTAA AGTGATTGAC TTTTTTGTGA CCTTTGAAAA TAATAGAGAT
    GTCGCTGAAG TTCTTGTTGT ACTACTTGAA GAGGAACTCT GCGCTTACGA TCTTACTGAC
    CCTAATATTT GTGCTATCAA AGCGCCATAT CTTCACTCTG TCCATGCATC AGCTGTCACT
    TGCAATTACC TTGCTTCTGA AGTCGTACAG TCGGTATATG AAAGTATTTT AAGAGCTGGA
    GATGAACAAG ACATTGACTA TAGCAATATT AGCTGGCCTA TCACTGGCGG TACTCTCCCG
    GATAACTTAG AAGAATCTGT AGAAGAGGAC GCGACTAAGC TTTATGAGAT TTTGTTAACT
    GGTCACGAAG ATGGTTCTGT TAAATTTTGG GACTGCACTG GAGTGTTGCT TAAACCAATT
    TATAATTTTA AAACTAGCAG CATTTTTGGA AGTGAGTCAG ACTTCCGAGA TGACGCAGCT
    GCAGATATGA GTGCGGAACA AGTCGATGAA GGAGAACCGC CATTTCGGAA ATCAGGACTT
    TTTGATCCTT ATTCAGATGA CCCTCGTTTA GCAGTGAAGA AAATAGCATT CTGCCCAAAA
    ACCGGACAAC TTATTGTTGG TGGCACAGCG GGCCAAATAG TTATAGCCGA CTTCATAGAC
    TTACCCGAAA AAGTGTCTTT AAAATACATT TCAATGAATT TGGTCAGCGA TCGTGATGGA
    TTTGTGTGGA AGGGTCACGA TCAGTTAAAC GTGCGATCGA ACTTATTAGA CGGAGAAGCA
    ATTCCTACGA CGGAACGTGG TGTAAATATA TCGGGAGTAC TGCAAGTTTT GCCGCCAGCC
    AGCATAACAT GCATGGCACT CGAAGCAAGC TGGGGCCTAG TATCTGGTGG GACTGCGCAC
    GGCTTAGTTC TCTTTGACTT CAAAAACTTT GTTCCAGTAT TTCATCGCTG CACTTTAAAC
    CCAAATGATC TTACTGGAGC AGGAGAGCAG CTGTCTCGTC GAAAGTCTTT TAAGAAATCA
    TTGAGGGAGT CATTTAGAAA GCTTCGCAAG GGTCGATCGA CCAGGACCAA CCAGAGCAAT
    CAAGTACCAA CAACGCTGGA AGCAAGACCC GTCGAGAGGC AAATAGAGGC TCGTTGTGCA
    GATGACGGGC TAGGATCCAT GGTGCGATGT TTACTATTTG CCAAAACTTA TGTTACTAAT
    GTCAACATAA CGTCGCCAAC TTTGTGGTCA GCAACAAATG CCAGTACAGT CTCGGTTTTC
    CTTCTGCATT TGCCACCAGC GCAGACCGCG GCAACTGCCG TCCCGTCGGC AAGTGGCAAT
    GCACCACCAC ACATGCCCCG CCGAATTTCT GCGCAGCTTG CTAAAGAAAT ACAATTAAAA
    CATCGTGCTC CTGTGGTGGG TATTTCTATT TTTGATCAGG CGGGTAGCCC TGTCGATCAG
    CTGAACGCCG GTGAAAACGG GAGTCCACCG CATCGTGTAC TTATTGCTTC CGAGGAACAG
    TTCAAGGTGT TTTCACTTCC GCAACTAAAG CCGATTAACA AATATAAGCT TACCGCTAAC
    GAAGGTGCTC GGATTCGCCG CATCCATTTT GGTTCGTTTA GTTGTCGCAT ATCCCCGGAA
    ACACTGCAGA GTATGCACGG TTGTAGCCCA ACTAAGTCCA CGCGTTCACA TGGCGATGGA
    GAGGCGGATC CTAATATCAG TGGAAGCTTG GCTGTAAGTC GTGGAGATGT ATATAACGAA
    ACAGCATTGA TATGTTTAAC GAATATGGGC GATATCATGG TTTTATCAGT ACCTGAATTA
    AAAAGACAGC TGAATGCCGC AGCAGTGCGA CGGGAAGACA TTAATGGAGT TTCGTCACTT
    TGCTTTACAA ACTCTGGAGA AGCACTGTAT ATGATGTCTT CTTCTGAACT GCAGCGTATT
    GCTTTAGCCA CGTCCAGAGT CGTGCAACCC ACTGGCGTTG TTCCAGTAGA ACCATTAGAA
    AATGAAGAGT CTGTGTTGGA AGAAAATGAT GCAGAGAATA ATAAGGAAAC CTACGCATGT
    GATGAAGTTG TGAATACATA TGAAATTAAA AATCCATCAG GCATTTCAAT ATGCACAAGG
    CCTGCAGAGG AAAACGTTGG AAGAAATAGT GTTCAGCAAG TTAATGGAGT CAACATTTCA
    AATTCACCTA ATCAAGCTAA CGAGACTATC AGCAGCTCTA TTGGCGATAT TACCGTTGAC
    TCGGTGCGCG ACCATTTAAA TATGACGACC ACCACTTTGT GTTCTATTAA TACAGAGGAA
    ACCATTGGTC GCCTATCTGT ACTTAGCACG CAAACCAACA AAGCCAGTAC TACCGTAAAC
    ATGAGTGAAA TTCCAAATAT TAATATTTCT AATTTAGAGG ACTTGGAATC GAAAAGAAAT
    ACGACGGAAA CGAGTACTAG TTCTGTTGTA ATTAAATCTA TAATTACAAA CATTTCTCAT
    GAAAAAACGA ACGGAGACAA CAAAATAGGA ACGCCAAAAA CAGCGCCTGA AGAAAGCCAA
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa13291
    Clone ID Related Accession (Same CDS sequence) NM_078715.4 , NM_164349.3 , NM_001258873.2 , NM_001258872.2 , NM_001258874.2 , NM_001258875.2
    Accession Version NM_001258874.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3486bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product lethal (2) giant larvae, isoform I
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001258874.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTTAAAGT TTATCAGAGG AAAAGGGCAG CAGCCCAGTG CTGACAGACA CCGCCTACAG 
    AAGGACCTTT TTGCTTATCG TAAGACGGCA CAGCATGGCT TTCCTCATAA GCCTTCGGCT
    CTTGCGTATG ATCCAGTTTT GAAACTTATG GCAATAGGGA CGCAAACAGG GGCTTTAAAA
    GTTTTCGGTC AACCCGGAGT TGAATTGTAC GGTCAGCATA CTTTGTTAAA CAATTCAGCA
    TCGGAGCTTA ATGTACAATT ACTTGAATGG GTGTATGGAA CTGGTCGCAT ACTTTCGTTG
    ACGGCAGCGA ATCAATTAAT TCTATGGGAG CCAGTTGGAG CAACGTTGCT GCCAATCAAA
    ACACTACCGT TTGACGGCAA ACTTAAAAAA GTTTCATCGC TGTGCTGTTC TCTCAGTAAG
    GATCTGCTAT GGATTGGAAC AGAAGGTGGA AACATCTATC AACTGGATTT ACATACATTT
    ACCATTAAGG AGCCTGTAAT TTACCATGAC GTTGTGCTAG AGCAGGTGCC ACCAGCCTAC
    AAGCTAAATC CTGGTGCAAT TGAGTCAATC CGCCAACTTC CAAACTCCCC TAGCAAACTT
    CTAGTTGCAT ACAATCGCGG CCTTTGTGTT TTGTGGGATT TTGAAAGCGC ATCTGTCCAG
    CGAGCATACA TAGCCCCTGG ACATGGACAG AGCGTTGGTC TTACAGTGAA CTTCGAAGGA
    TCTGAATTTA CCTGGTACCA CGCTGATGGT TCATACGCCA CTTGGAGCAT AGATAACCCA
    GAACCGCCGT CGAATGTTAA TTATGTGCCT TATGGACCTG ATCCATGCAA AAGCATAAAT
    CGACTGTACA AAGGCAAGCG AAGATCCAAC GATGTAATTG TTTTTTCCGG CGGCATGCCA
    CGGTCAGCAT ATGGTGATCA CAATTGTGTG TCCGTTCACG CCAGCGATGG ACACAAAGTG
    TGTCTTGACT TTACGTCTAA AGTGATTGAC TTTTTTGTGA CCTTTGAAAA TAATAGAGAT
    GTCGCTGAAG TTCTTGTTGT ACTACTTGAA GAGGAACTCT GCGCTTACGA TCTTACTGAC
    CCTAATATTT GTGCTATCAA AGCGCCATAT CTTCACTCTG TCCATGCATC AGCTGTCACT
    TGCAATTACC TTGCTTCTGA AGTCGTACAG TCGGTATATG AAAGTATTTT AAGAGCTGGA
    GATGAACAAG ACATTGACTA TAGCAATATT AGCTGGCCTA TCACTGGCGG TACTCTCCCG
    GATAACTTAG AAGAATCTGT AGAAGAGGAC GCGACTAAGC TTTATGAGAT TTTGTTAACT
    GGTCACGAAG ATGGTTCTGT TAAATTTTGG GACTGCACTG GAGTGTTGCT TAAACCAATT
    TATAATTTTA AAACTAGCAG CATTTTTGGA AGTGAGTCAG ACTTCCGAGA TGACGCAGCT
    GCAGATATGA GTGCGGAACA AGTCGATGAA GGAGAACCGC CATTTCGGAA ATCAGGACTT
    TTTGATCCTT ATTCAGATGA CCCTCGTTTA GCAGTGAAGA AAATAGCATT CTGCCCAAAA
    ACCGGACAAC TTATTGTTGG TGGCACAGCG GGCCAAATAG TTATAGCCGA CTTCATAGAC
    TTACCCGAAA AAGTGTCTTT AAAATACATT TCAATGAATT TGGTCAGCGA TCGTGATGGA
    TTTGTGTGGA AGGGTCACGA TCAGTTAAAC GTGCGATCGA ACTTATTAGA CGGAGAAGCA
    ATTCCTACGA CGGAACGTGG TGTAAATATA TCGGGAGTAC TGCAAGTTTT GCCGCCAGCC
    AGCATAACAT GCATGGCACT CGAAGCAAGC TGGGGCCTAG TATCTGGTGG GACTGCGCAC
    GGCTTAGTTC TCTTTGACTT CAAAAACTTT GTTCCAGTAT TTCATCGCTG CACTTTAAAC
    CCAAATGATC TTACTGGAGC AGGAGAGCAG CTGTCTCGTC GAAAGTCTTT TAAGAAATCA
    TTGAGGGAGT CATTTAGAAA GCTTCGCAAG GGTCGATCGA CCAGGACCAA CCAGAGCAAT
    CAAGTACCAA CAACGCTGGA AGCAAGACCC GTCGAGAGGC AAATAGAGGC TCGTTGTGCA
    GATGACGGGC TAGGATCCAT GGTGCGATGT TTACTATTTG CCAAAACTTA TGTTACTAAT
    GTCAACATAA CGTCGCCAAC TTTGTGGTCA GCAACAAATG CCAGTACAGT CTCGGTTTTC
    CTTCTGCATT TGCCACCAGC GCAGACCGCG GCAACTGCCG TCCCGTCGGC AAGTGGCAAT
    GCACCACCAC ACATGCCCCG CCGAATTTCT GCGCAGCTTG CTAAAGAAAT ACAATTAAAA
    CATCGTGCTC CTGTGGTGGG TATTTCTATT TTTGATCAGG CGGGTAGCCC TGTCGATCAG
    CTGAACGCCG GTGAAAACGG GAGTCCACCG CATCGTGTAC TTATTGCTTC CGAGGAACAG
    TTCAAGGTGT TTTCACTTCC GCAACTAAAG CCGATTAACA AATATAAGCT TACCGCTAAC
    GAAGGTGCTC GGATTCGCCG CATCCATTTT GGTTCGTTTA GTTGTCGCAT ATCCCCGGAA
    ACACTGCAGA GTATGCACGG TTGTAGCCCA ACTAAGTCCA CGCGTTCACA TGGCGATGGA
    GAGGCGGATC CTAATATCAG TGGAAGCTTG GCTGTAAGTC GTGGAGATGT ATATAACGAA
    ACAGCATTGA TATGTTTAAC GAATATGGGC GATATCATGG TTTTATCAGT ACCTGAATTA
    AAAAGACAGC TGAATGCCGC AGCAGTGCGA CGGGAAGACA TTAATGGAGT TTCGTCACTT
    TGCTTTACAA ACTCTGGAGA AGCACTGTAT ATGATGTCTT CTTCTGAACT GCAGCGTATT
    GCTTTAGCCA CGTCCAGAGT CGTGCAACCC ACTGGCGTTG TTCCAGTAGA ACCATTAGAA
    AATGAAGAGT CTGTGTTGGA AGAAAATGAT GCAGAGAATA ATAAGGAAAC CTACGCATGT
    GATGAAGTTG TGAATACATA TGAAATTAAA AATCCATCAG GCATTTCAAT ATGCACAAGG
    CCTGCAGAGG AAAACGTTGG AAGAAATAGT GTTCAGCAAG TTAATGGAGT CAACATTTCA
    AATTCACCTA ATCAAGCTAA CGAGACTATC AGCAGCTCTA TTGGCGATAT TACCGTTGAC
    TCGGTGCGCG ACCATTTAAA TATGACGACC ACCACTTTGT GTTCTATTAA TACAGAGGAA
    ACCATTGGTC GCCTATCTGT ACTTAGCACG CAAACCAACA AAGCCAGTAC TACCGTAAAC
    ATGAGTGAAA TTCCAAATAT TAATATTTCT AATTTAGAGG ACTTGGAATC GAAAAGAAAT
    ACGACGGAAA CGAGTACTAG TTCTGTTGTA ATTAAATCTA TAATTACAAA CATTTCTCAT
    GAAAAAACGA ACGGAGACAA CAAAATAGGA ACGCCAAAAA CAGCGCCTGA AGAAAGCCAA
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001245803.1
    CDS529..4014
    Translation

    Target ORF information:

    RefSeq Version NM_001258874.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster lethal (2) giant larvae (l(2)gl), transcript variant I, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001258874.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTTAAAGT TTATCAGAGG AAAAGGGCAG CAGCCCAGTG CTGACAGACA CCGCCTACAG 
    AAGGACCTTT TTGCTTATCG TAAGACGGCA CAGCATGGCT TTCCTCATAA GCCTTCGGCT
    CTTGCGTATG ATCCAGTTTT GAAACTTATG GCAATAGGGA CGCAAACAGG GGCTTTAAAA
    GTTTTCGGTC AACCCGGAGT TGAATTGTAC GGTCAGCATA CTTTGTTAAA CAATTCAGCA
    TCGGAGCTTA ATGTACAATT ACTTGAATGG GTGTATGGAA CTGGTCGCAT ACTTTCGTTG
    ACGGCAGCGA ATCAATTAAT TCTATGGGAG CCAGTTGGAG CAACGTTGCT GCCAATCAAA
    ACACTACCGT TTGACGGCAA ACTTAAAAAA GTTTCATCGC TGTGCTGTTC TCTCAGTAAG
    GATCTGCTAT GGATTGGAAC AGAAGGTGGA AACATCTATC AACTGGATTT ACATACATTT
    ACCATTAAGG AGCCTGTAAT TTACCATGAC GTTGTGCTAG AGCAGGTGCC ACCAGCCTAC
    AAGCTAAATC CTGGTGCAAT TGAGTCAATC CGCCAACTTC CAAACTCCCC TAGCAAACTT
    CTAGTTGCAT ACAATCGCGG CCTTTGTGTT TTGTGGGATT TTGAAAGCGC ATCTGTCCAG
    CGAGCATACA TAGCCCCTGG ACATGGACAG AGCGTTGGTC TTACAGTGAA CTTCGAAGGA
    TCTGAATTTA CCTGGTACCA CGCTGATGGT TCATACGCCA CTTGGAGCAT AGATAACCCA
    GAACCGCCGT CGAATGTTAA TTATGTGCCT TATGGACCTG ATCCATGCAA AAGCATAAAT
    CGACTGTACA AAGGCAAGCG AAGATCCAAC GATGTAATTG TTTTTTCCGG CGGCATGCCA
    CGGTCAGCAT ATGGTGATCA CAATTGTGTG TCCGTTCACG CCAGCGATGG ACACAAAGTG
    TGTCTTGACT TTACGTCTAA AGTGATTGAC TTTTTTGTGA CCTTTGAAAA TAATAGAGAT
    GTCGCTGAAG TTCTTGTTGT ACTACTTGAA GAGGAACTCT GCGCTTACGA TCTTACTGAC
    CCTAATATTT GTGCTATCAA AGCGCCATAT CTTCACTCTG TCCATGCATC AGCTGTCACT
    TGCAATTACC TTGCTTCTGA AGTCGTACAG TCGGTATATG AAAGTATTTT AAGAGCTGGA
    GATGAACAAG ACATTGACTA TAGCAATATT AGCTGGCCTA TCACTGGCGG TACTCTCCCG
    GATAACTTAG AAGAATCTGT AGAAGAGGAC GCGACTAAGC TTTATGAGAT TTTGTTAACT
    GGTCACGAAG ATGGTTCTGT TAAATTTTGG GACTGCACTG GAGTGTTGCT TAAACCAATT
    TATAATTTTA AAACTAGCAG CATTTTTGGA AGTGAGTCAG ACTTCCGAGA TGACGCAGCT
    GCAGATATGA GTGCGGAACA AGTCGATGAA GGAGAACCGC CATTTCGGAA ATCAGGACTT
    TTTGATCCTT ATTCAGATGA CCCTCGTTTA GCAGTGAAGA AAATAGCATT CTGCCCAAAA
    ACCGGACAAC TTATTGTTGG TGGCACAGCG GGCCAAATAG TTATAGCCGA CTTCATAGAC
    TTACCCGAAA AAGTGTCTTT AAAATACATT TCAATGAATT TGGTCAGCGA TCGTGATGGA
    TTTGTGTGGA AGGGTCACGA TCAGTTAAAC GTGCGATCGA ACTTATTAGA CGGAGAAGCA
    ATTCCTACGA CGGAACGTGG TGTAAATATA TCGGGAGTAC TGCAAGTTTT GCCGCCAGCC
    AGCATAACAT GCATGGCACT CGAAGCAAGC TGGGGCCTAG TATCTGGTGG GACTGCGCAC
    GGCTTAGTTC TCTTTGACTT CAAAAACTTT GTTCCAGTAT TTCATCGCTG CACTTTAAAC
    CCAAATGATC TTACTGGAGC AGGAGAGCAG CTGTCTCGTC GAAAGTCTTT TAAGAAATCA
    TTGAGGGAGT CATTTAGAAA GCTTCGCAAG GGTCGATCGA CCAGGACCAA CCAGAGCAAT
    CAAGTACCAA CAACGCTGGA AGCAAGACCC GTCGAGAGGC AAATAGAGGC TCGTTGTGCA
    GATGACGGGC TAGGATCCAT GGTGCGATGT TTACTATTTG CCAAAACTTA TGTTACTAAT
    GTCAACATAA CGTCGCCAAC TTTGTGGTCA GCAACAAATG CCAGTACAGT CTCGGTTTTC
    CTTCTGCATT TGCCACCAGC GCAGACCGCG GCAACTGCCG TCCCGTCGGC AAGTGGCAAT
    GCACCACCAC ACATGCCCCG CCGAATTTCT GCGCAGCTTG CTAAAGAAAT ACAATTAAAA
    CATCGTGCTC CTGTGGTGGG TATTTCTATT TTTGATCAGG CGGGTAGCCC TGTCGATCAG
    CTGAACGCCG GTGAAAACGG GAGTCCACCG CATCGTGTAC TTATTGCTTC CGAGGAACAG
    TTCAAGGTGT TTTCACTTCC GCAACTAAAG CCGATTAACA AATATAAGCT TACCGCTAAC
    GAAGGTGCTC GGATTCGCCG CATCCATTTT GGTTCGTTTA GTTGTCGCAT ATCCCCGGAA
    ACACTGCAGA GTATGCACGG TTGTAGCCCA ACTAAGTCCA CGCGTTCACA TGGCGATGGA
    GAGGCGGATC CTAATATCAG TGGAAGCTTG GCTGTAAGTC GTGGAGATGT ATATAACGAA
    ACAGCATTGA TATGTTTAAC GAATATGGGC GATATCATGG TTTTATCAGT ACCTGAATTA
    AAAAGACAGC TGAATGCCGC AGCAGTGCGA CGGGAAGACA TTAATGGAGT TTCGTCACTT
    TGCTTTACAA ACTCTGGAGA AGCACTGTAT ATGATGTCTT CTTCTGAACT GCAGCGTATT
    GCTTTAGCCA CGTCCAGAGT CGTGCAACCC ACTGGCGTTG TTCCAGTAGA ACCATTAGAA
    AATGAAGAGT CTGTGTTGGA AGAAAATGAT GCAGAGAATA ATAAGGAAAC CTACGCATGT
    GATGAAGTTG TGAATACATA TGAAATTAAA AATCCATCAG GCATTTCAAT ATGCACAAGG
    CCTGCAGAGG AAAACGTTGG AAGAAATAGT GTTCAGCAAG TTAATGGAGT CAACATTTCA
    AATTCACCTA ATCAAGCTAA CGAGACTATC AGCAGCTCTA TTGGCGATAT TACCGTTGAC
    TCGGTGCGCG ACCATTTAAA TATGACGACC ACCACTTTGT GTTCTATTAA TACAGAGGAA
    ACCATTGGTC GCCTATCTGT ACTTAGCACG CAAACCAACA AAGCCAGTAC TACCGTAAAC
    ATGAGTGAAA TTCCAAATAT TAATATTTCT AATTTAGAGG ACTTGGAATC GAAAAGAAAT
    ACGACGGAAA CGAGTACTAG TTCTGTTGTA ATTAAATCTA TAATTACAAA CATTTCTCAT
    GAAAAAACGA ACGGAGACAA CAAAATAGGA ACGCCAAAAA CAGCGCCTGA AGAAAGCCAA
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa13291
    Clone ID Related Accession (Same CDS sequence) NM_078715.4 , NM_164349.3 , NM_001258873.2 , NM_001258872.2 , NM_001258874.2 , NM_001258875.2
    Accession Version NM_001258875.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3486bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product lethal (2) giant larvae, isoform J
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001258875.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTTAAAGT TTATCAGAGG AAAAGGGCAG CAGCCCAGTG CTGACAGACA CCGCCTACAG 
    AAGGACCTTT TTGCTTATCG TAAGACGGCA CAGCATGGCT TTCCTCATAA GCCTTCGGCT
    CTTGCGTATG ATCCAGTTTT GAAACTTATG GCAATAGGGA CGCAAACAGG GGCTTTAAAA
    GTTTTCGGTC AACCCGGAGT TGAATTGTAC GGTCAGCATA CTTTGTTAAA CAATTCAGCA
    TCGGAGCTTA ATGTACAATT ACTTGAATGG GTGTATGGAA CTGGTCGCAT ACTTTCGTTG
    ACGGCAGCGA ATCAATTAAT TCTATGGGAG CCAGTTGGAG CAACGTTGCT GCCAATCAAA
    ACACTACCGT TTGACGGCAA ACTTAAAAAA GTTTCATCGC TGTGCTGTTC TCTCAGTAAG
    GATCTGCTAT GGATTGGAAC AGAAGGTGGA AACATCTATC AACTGGATTT ACATACATTT
    ACCATTAAGG AGCCTGTAAT TTACCATGAC GTTGTGCTAG AGCAGGTGCC ACCAGCCTAC
    AAGCTAAATC CTGGTGCAAT TGAGTCAATC CGCCAACTTC CAAACTCCCC TAGCAAACTT
    CTAGTTGCAT ACAATCGCGG CCTTTGTGTT TTGTGGGATT TTGAAAGCGC ATCTGTCCAG
    CGAGCATACA TAGCCCCTGG ACATGGACAG AGCGTTGGTC TTACAGTGAA CTTCGAAGGA
    TCTGAATTTA CCTGGTACCA CGCTGATGGT TCATACGCCA CTTGGAGCAT AGATAACCCA
    GAACCGCCGT CGAATGTTAA TTATGTGCCT TATGGACCTG ATCCATGCAA AAGCATAAAT
    CGACTGTACA AAGGCAAGCG AAGATCCAAC GATGTAATTG TTTTTTCCGG CGGCATGCCA
    CGGTCAGCAT ATGGTGATCA CAATTGTGTG TCCGTTCACG CCAGCGATGG ACACAAAGTG
    TGTCTTGACT TTACGTCTAA AGTGATTGAC TTTTTTGTGA CCTTTGAAAA TAATAGAGAT
    GTCGCTGAAG TTCTTGTTGT ACTACTTGAA GAGGAACTCT GCGCTTACGA TCTTACTGAC
    CCTAATATTT GTGCTATCAA AGCGCCATAT CTTCACTCTG TCCATGCATC AGCTGTCACT
    TGCAATTACC TTGCTTCTGA AGTCGTACAG TCGGTATATG AAAGTATTTT AAGAGCTGGA
    GATGAACAAG ACATTGACTA TAGCAATATT AGCTGGCCTA TCACTGGCGG TACTCTCCCG
    GATAACTTAG AAGAATCTGT AGAAGAGGAC GCGACTAAGC TTTATGAGAT TTTGTTAACT
    GGTCACGAAG ATGGTTCTGT TAAATTTTGG GACTGCACTG GAGTGTTGCT TAAACCAATT
    TATAATTTTA AAACTAGCAG CATTTTTGGA AGTGAGTCAG ACTTCCGAGA TGACGCAGCT
    GCAGATATGA GTGCGGAACA AGTCGATGAA GGAGAACCGC CATTTCGGAA ATCAGGACTT
    TTTGATCCTT ATTCAGATGA CCCTCGTTTA GCAGTGAAGA AAATAGCATT CTGCCCAAAA
    ACCGGACAAC TTATTGTTGG TGGCACAGCG GGCCAAATAG TTATAGCCGA CTTCATAGAC
    TTACCCGAAA AAGTGTCTTT AAAATACATT TCAATGAATT TGGTCAGCGA TCGTGATGGA
    TTTGTGTGGA AGGGTCACGA TCAGTTAAAC GTGCGATCGA ACTTATTAGA CGGAGAAGCA
    ATTCCTACGA CGGAACGTGG TGTAAATATA TCGGGAGTAC TGCAAGTTTT GCCGCCAGCC
    AGCATAACAT GCATGGCACT CGAAGCAAGC TGGGGCCTAG TATCTGGTGG GACTGCGCAC
    GGCTTAGTTC TCTTTGACTT CAAAAACTTT GTTCCAGTAT TTCATCGCTG CACTTTAAAC
    CCAAATGATC TTACTGGAGC AGGAGAGCAG CTGTCTCGTC GAAAGTCTTT TAAGAAATCA
    TTGAGGGAGT CATTTAGAAA GCTTCGCAAG GGTCGATCGA CCAGGACCAA CCAGAGCAAT
    CAAGTACCAA CAACGCTGGA AGCAAGACCC GTCGAGAGGC AAATAGAGGC TCGTTGTGCA
    GATGACGGGC TAGGATCCAT GGTGCGATGT TTACTATTTG CCAAAACTTA TGTTACTAAT
    GTCAACATAA CGTCGCCAAC TTTGTGGTCA GCAACAAATG CCAGTACAGT CTCGGTTTTC
    CTTCTGCATT TGCCACCAGC GCAGACCGCG GCAACTGCCG TCCCGTCGGC AAGTGGCAAT
    GCACCACCAC ACATGCCCCG CCGAATTTCT GCGCAGCTTG CTAAAGAAAT ACAATTAAAA
    CATCGTGCTC CTGTGGTGGG TATTTCTATT TTTGATCAGG CGGGTAGCCC TGTCGATCAG
    CTGAACGCCG GTGAAAACGG GAGTCCACCG CATCGTGTAC TTATTGCTTC CGAGGAACAG
    TTCAAGGTGT TTTCACTTCC GCAACTAAAG CCGATTAACA AATATAAGCT TACCGCTAAC
    GAAGGTGCTC GGATTCGCCG CATCCATTTT GGTTCGTTTA GTTGTCGCAT ATCCCCGGAA
    ACACTGCAGA GTATGCACGG TTGTAGCCCA ACTAAGTCCA CGCGTTCACA TGGCGATGGA
    GAGGCGGATC CTAATATCAG TGGAAGCTTG GCTGTAAGTC GTGGAGATGT ATATAACGAA
    ACAGCATTGA TATGTTTAAC GAATATGGGC GATATCATGG TTTTATCAGT ACCTGAATTA
    AAAAGACAGC TGAATGCCGC AGCAGTGCGA CGGGAAGACA TTAATGGAGT TTCGTCACTT
    TGCTTTACAA ACTCTGGAGA AGCACTGTAT ATGATGTCTT CTTCTGAACT GCAGCGTATT
    GCTTTAGCCA CGTCCAGAGT CGTGCAACCC ACTGGCGTTG TTCCAGTAGA ACCATTAGAA
    AATGAAGAGT CTGTGTTGGA AGAAAATGAT GCAGAGAATA ATAAGGAAAC CTACGCATGT
    GATGAAGTTG TGAATACATA TGAAATTAAA AATCCATCAG GCATTTCAAT ATGCACAAGG
    CCTGCAGAGG AAAACGTTGG AAGAAATAGT GTTCAGCAAG TTAATGGAGT CAACATTTCA
    AATTCACCTA ATCAAGCTAA CGAGACTATC AGCAGCTCTA TTGGCGATAT TACCGTTGAC
    TCGGTGCGCG ACCATTTAAA TATGACGACC ACCACTTTGT GTTCTATTAA TACAGAGGAA
    ACCATTGGTC GCCTATCTGT ACTTAGCACG CAAACCAACA AAGCCAGTAC TACCGTAAAC
    ATGAGTGAAA TTCCAAATAT TAATATTTCT AATTTAGAGG ACTTGGAATC GAAAAGAAAT
    ACGACGGAAA CGAGTACTAG TTCTGTTGTA ATTAAATCTA TAATTACAAA CATTTCTCAT
    GAAAAAACGA ACGGAGACAA CAAAATAGGA ACGCCAAAAA CAGCGCCTGA AGAAAGCCAA
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001245804.1
    CDS667..4152
    Translation

    Target ORF information:

    RefSeq Version NM_001258875.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster lethal (2) giant larvae (l(2)gl), transcript variant J, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001258875.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTTAAAGT TTATCAGAGG AAAAGGGCAG CAGCCCAGTG CTGACAGACA CCGCCTACAG 
    AAGGACCTTT TTGCTTATCG TAAGACGGCA CAGCATGGCT TTCCTCATAA GCCTTCGGCT
    CTTGCGTATG ATCCAGTTTT GAAACTTATG GCAATAGGGA CGCAAACAGG GGCTTTAAAA
    GTTTTCGGTC AACCCGGAGT TGAATTGTAC GGTCAGCATA CTTTGTTAAA CAATTCAGCA
    TCGGAGCTTA ATGTACAATT ACTTGAATGG GTGTATGGAA CTGGTCGCAT ACTTTCGTTG
    ACGGCAGCGA ATCAATTAAT TCTATGGGAG CCAGTTGGAG CAACGTTGCT GCCAATCAAA
    ACACTACCGT TTGACGGCAA ACTTAAAAAA GTTTCATCGC TGTGCTGTTC TCTCAGTAAG
    GATCTGCTAT GGATTGGAAC AGAAGGTGGA AACATCTATC AACTGGATTT ACATACATTT
    ACCATTAAGG AGCCTGTAAT TTACCATGAC GTTGTGCTAG AGCAGGTGCC ACCAGCCTAC
    AAGCTAAATC CTGGTGCAAT TGAGTCAATC CGCCAACTTC CAAACTCCCC TAGCAAACTT
    CTAGTTGCAT ACAATCGCGG CCTTTGTGTT TTGTGGGATT TTGAAAGCGC ATCTGTCCAG
    CGAGCATACA TAGCCCCTGG ACATGGACAG AGCGTTGGTC TTACAGTGAA CTTCGAAGGA
    TCTGAATTTA CCTGGTACCA CGCTGATGGT TCATACGCCA CTTGGAGCAT AGATAACCCA
    GAACCGCCGT CGAATGTTAA TTATGTGCCT TATGGACCTG ATCCATGCAA AAGCATAAAT
    CGACTGTACA AAGGCAAGCG AAGATCCAAC GATGTAATTG TTTTTTCCGG CGGCATGCCA
    CGGTCAGCAT ATGGTGATCA CAATTGTGTG TCCGTTCACG CCAGCGATGG ACACAAAGTG
    TGTCTTGACT TTACGTCTAA AGTGATTGAC TTTTTTGTGA CCTTTGAAAA TAATAGAGAT
    GTCGCTGAAG TTCTTGTTGT ACTACTTGAA GAGGAACTCT GCGCTTACGA TCTTACTGAC
    CCTAATATTT GTGCTATCAA AGCGCCATAT CTTCACTCTG TCCATGCATC AGCTGTCACT
    TGCAATTACC TTGCTTCTGA AGTCGTACAG TCGGTATATG AAAGTATTTT AAGAGCTGGA
    GATGAACAAG ACATTGACTA TAGCAATATT AGCTGGCCTA TCACTGGCGG TACTCTCCCG
    GATAACTTAG AAGAATCTGT AGAAGAGGAC GCGACTAAGC TTTATGAGAT TTTGTTAACT
    GGTCACGAAG ATGGTTCTGT TAAATTTTGG GACTGCACTG GAGTGTTGCT TAAACCAATT
    TATAATTTTA AAACTAGCAG CATTTTTGGA AGTGAGTCAG ACTTCCGAGA TGACGCAGCT
    GCAGATATGA GTGCGGAACA AGTCGATGAA GGAGAACCGC CATTTCGGAA ATCAGGACTT
    TTTGATCCTT ATTCAGATGA CCCTCGTTTA GCAGTGAAGA AAATAGCATT CTGCCCAAAA
    ACCGGACAAC TTATTGTTGG TGGCACAGCG GGCCAAATAG TTATAGCCGA CTTCATAGAC
    TTACCCGAAA AAGTGTCTTT AAAATACATT TCAATGAATT TGGTCAGCGA TCGTGATGGA
    TTTGTGTGGA AGGGTCACGA TCAGTTAAAC GTGCGATCGA ACTTATTAGA CGGAGAAGCA
    ATTCCTACGA CGGAACGTGG TGTAAATATA TCGGGAGTAC TGCAAGTTTT GCCGCCAGCC
    AGCATAACAT GCATGGCACT CGAAGCAAGC TGGGGCCTAG TATCTGGTGG GACTGCGCAC
    GGCTTAGTTC TCTTTGACTT CAAAAACTTT GTTCCAGTAT TTCATCGCTG CACTTTAAAC
    CCAAATGATC TTACTGGAGC AGGAGAGCAG CTGTCTCGTC GAAAGTCTTT TAAGAAATCA
    TTGAGGGAGT CATTTAGAAA GCTTCGCAAG GGTCGATCGA CCAGGACCAA CCAGAGCAAT
    CAAGTACCAA CAACGCTGGA AGCAAGACCC GTCGAGAGGC AAATAGAGGC TCGTTGTGCA
    GATGACGGGC TAGGATCCAT GGTGCGATGT TTACTATTTG CCAAAACTTA TGTTACTAAT
    GTCAACATAA CGTCGCCAAC TTTGTGGTCA GCAACAAATG CCAGTACAGT CTCGGTTTTC
    CTTCTGCATT TGCCACCAGC GCAGACCGCG GCAACTGCCG TCCCGTCGGC AAGTGGCAAT
    GCACCACCAC ACATGCCCCG CCGAATTTCT GCGCAGCTTG CTAAAGAAAT ACAATTAAAA
    CATCGTGCTC CTGTGGTGGG TATTTCTATT TTTGATCAGG CGGGTAGCCC TGTCGATCAG
    CTGAACGCCG GTGAAAACGG GAGTCCACCG CATCGTGTAC TTATTGCTTC CGAGGAACAG
    TTCAAGGTGT TTTCACTTCC GCAACTAAAG CCGATTAACA AATATAAGCT TACCGCTAAC
    GAAGGTGCTC GGATTCGCCG CATCCATTTT GGTTCGTTTA GTTGTCGCAT ATCCCCGGAA
    ACACTGCAGA GTATGCACGG TTGTAGCCCA ACTAAGTCCA CGCGTTCACA TGGCGATGGA
    GAGGCGGATC CTAATATCAG TGGAAGCTTG GCTGTAAGTC GTGGAGATGT ATATAACGAA
    ACAGCATTGA TATGTTTAAC GAATATGGGC GATATCATGG TTTTATCAGT ACCTGAATTA
    AAAAGACAGC TGAATGCCGC AGCAGTGCGA CGGGAAGACA TTAATGGAGT TTCGTCACTT
    TGCTTTACAA ACTCTGGAGA AGCACTGTAT ATGATGTCTT CTTCTGAACT GCAGCGTATT
    GCTTTAGCCA CGTCCAGAGT CGTGCAACCC ACTGGCGTTG TTCCAGTAGA ACCATTAGAA
    AATGAAGAGT CTGTGTTGGA AGAAAATGAT GCAGAGAATA ATAAGGAAAC CTACGCATGT
    GATGAAGTTG TGAATACATA TGAAATTAAA AATCCATCAG GCATTTCAAT ATGCACAAGG
    CCTGCAGAGG AAAACGTTGG AAGAAATAGT GTTCAGCAAG TTAATGGAGT CAACATTTCA
    AATTCACCTA ATCAAGCTAA CGAGACTATC AGCAGCTCTA TTGGCGATAT TACCGTTGAC
    TCGGTGCGCG ACCATTTAAA TATGACGACC ACCACTTTGT GTTCTATTAA TACAGAGGAA
    ACCATTGGTC GCCTATCTGT ACTTAGCACG CAAACCAACA AAGCCAGTAC TACCGTAAAC
    ATGAGTGAAA TTCCAAATAT TAATATTTCT AATTTAGAGG ACTTGGAATC GAAAAGAAAT
    ACGACGGAAA CGAGTACTAG TTCTGTTGTA ATTAAATCTA TAATTACAAA CATTTCTCAT
    GAAAAAACGA ACGGAGACAA CAAAATAGGA ACGCCAAAAA CAGCGCCTGA AGAAAGCCAA
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa13292
    Clone ID Related Accession (Same CDS sequence) NM_001272858.1
    Accession Version NM_001272858.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3282bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product lethal (2) giant larvae, isoform K
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTTAAAGT TTATCAGAGG AAAAGGGCAG CAGCCCAGTG CTGACAGACA CCGCCTACAG 
    AAGGACCTTT TTGCTTATCG TAAGACGGCA CAGCATGGCT TTCCTCATAA GCCTTCGGCT
    CTTGCGTATG ATCCAGTTTT GAAACTTATG GCAATAGGGA CGCAAACAGG GGCTTTAAAA
    GTTTTCGGTC AACCCGGAGT TGAATTGTAC GGTCAGCATA CTTTGTTAAA CAATTCAGCA
    TCGGAGCTTA ATGTACAATT ACTTGAATGG GTGTATGGAA CTGGTCGCAT ACTTTCGTTG
    ACGGCAGCGA ATCAATTAAT TCTATGGGAG CCAGTTGGAG CAACGTTGCT GCCAATCAAA
    ACACTACCGT TTGACGGCAA ACTTAAAAAA GTTTCATCGC TGTGCTGTTC TCTCAGTAAG
    GATCTGCTAT GGATTGGAAC AGAAGGTGGA AACATCTATC AACTGGATTT ACATACATTT
    ACCATTAAGG AGCCTGTAAT TTACCATGAC GTTGTGCTAG AGCAGGTGCC ACCAGCCTAC
    AAGCTAAATC CTGGTGCAAT TGAGTCAATC CGCCAACTTC CAAACTCCCC TAGCAAACTT
    CTAGTTGCAT ACAATCGCGG CCTTTGTGTT TTGTGGGATT TTGAAAGCGC ATCTGTCCAG
    CGAGCATACA TAGCCCCTGG ACATGGACAG AGCGTTGGTC TTACAGTGAA CTTCGAAGGA
    TCTGAATTTA CCTGGTACCA CGCTGATGGT TCATACGCCA CTTGGAGCAT AGATAACCCA
    GAACCGCCGT CGAATGTTAA TTATGTGCCT TATGGACCTG ATCCATGCAA AAGCATAAAT
    CGACTGTACA AAGGCAAGCG AAGATCCAAC GATGTAATTG TTTTTTCCGG CGGCATGCCA
    CGGTCAGCAT ATGGTGATCA CAATTGTGTG TCCGTTCACG CCAGCGATGG ACACAAAGTG
    TGTCTTGACT TTACGTCTAA AGTGATTGAC TTTTTTGTGA CCTTTGAAAA TAATAGAGAT
    GTCGCTGAAG TTCTTGTTGT ACTACTTGAA GAGGAACTCT GCGCTTACGA TCTTACTGAC
    CCTAATATTT GTGCTATCAA AGCGCCATAT CTTCACTCTG TCCATGCATC AGCTGTCACT
    TGCAATTACC TTGCTTCTGA AGTCGTACAG TCGGTATATG AAAGTATTTT AAGAGCTGGA
    GATGAACAAG ACATTGACTA TAGCAATATT AGCTGGCCTA TCACTGGCGG TACTCTCCCG
    GATAACTTAG AAGAATCTGT AGAAGAGGAC GCGACTAAGC TTTATGAGAT TTTGTTAACT
    GGTCACGAAG ATGGTTCTGT TAAATTTTGG GACTGCACTG GAGTGTTGCT TAAACCAATT
    TATAATTTTA AAACTAGCAG CATTTTTGGA AGTGAGTCAG ACTTCCGAGA TGACGCAGCT
    GCAGATATGA GTGCGGAACA AGTCGATGAA GGAGAACCGC CATTTCGGAA ATCAGGACTT
    TTTGATCCTT ATTCAGATGA CCCTCGTTTA GCAGTGAAGA AAATAGCATT CTGCCCAAAA
    ACCGGACAAC TTATTGTTGG TGGCACAGCG GGCCAAATAG TTATAGCCGA CTTCATAGAC
    TTACCCGAAA AAGTGTCTTT AAAATACATT TCAATGAATT TGGTCAGCGA TCGTGATGGA
    TTTGTGTGGA AGGGTCACGA TCAGTTAAAC GTGCGATCGA ACTTATTAGA CGGAGAAGCA
    ATTCCTACGA CGGAACGTGG TGTAAATATA TCGGGAGTAC TGCAAGTTTT GCCGCCAGCC
    AGCATAACAT GCATGGCACT CGAAGCAAGC TGGGGCCTAG TATCTGGTGG GACTGCGCAC
    GGCTTAGTTC TCTTTGACTT CAAAAACTTT GTTCCAGTAT TTCATCGCTG CACTTTAAAC
    CCAAATGATC TTACTGGAGC AGGAGAGCAG CTGTCTCGTC GAAAGTCTTT TAAGAAATCA
    TTGAGGGAGT CATTTAGAAA GCTTCGCAAG GGTCGATCGA CCAGGACCAA CCAGAGCAAT
    CAAGTACCAA CAACGCTGGA AGCAAGACCC GTCGAGAGGC AAATAGAGGC TCGTTGTGCA
    GATGACGGGC TAGGATCCAT GGTGCGATGT TTACTATTTG CCAAAACTTA TGTTACTAAT
    GTCAACATAA CGTCGCCAAC TTTGTGGTCA GCAACAAATG CCAGTACAGT CTCGGTTTTC
    CTTCTGCATT TGCCACCAGC GCAGACCGCG GCAACTGCCG TCCCGTCGGC AAGTGGCAAT
    GCACCACCAC ACATGCCCCG CCGAATTTCT GCGCAGCTTG CTAAAGAAAT ACAATTAAAA
    CATCGTGCTC CTGTGGTGGG TATTTCTATT TTTGATCAGG CGGGTAGCCC TGTCGATCAG
    CTGAACGCCG GTGAAAACGG GAGTCCACCG CATCGTGTAC TTATTGCTTC CGAGGAACAG
    TTCAAGGTGT TTTCACTTCC GCAACTAAAG CCGATTAACA AATATAAGCT TACCGCTAAC
    GAAGGTGCTC GGATTCGCCG CATCCATTTT GGTTCGTTTA GTTGTCGCAT ATCCCCGGAA
    ACACTGCAGA GTATGCACGG TTGTAGCCCA ACTAAGTCCA CGCGTTCACA TGGCGATGGA
    GAGGCGGATC CTAATATCAG TGGAAGCTTG GCTGTAAGTC GTGGAGATGT ATATAACGAA
    ACAGCATTGA TATGTTTAAC GAATATGGGC GATATCATGG TTTTATCAGT ACCTGAATTA
    AAAAGACAGC TGAATGCCGC AGCAGTGCGA CGGGAAGACA TTAATGGAGT TTCGTCACTT
    TGCTTTACAA ACTCTGGAGA AGCACTGTAT ATGATGTCTT CTTCTGAACT GCAGCGTATT
    GCTTTAGCCA CGTCCAGAGT CGTGCAACCC ACTGGCGTTG TTCCAGTAGA ACCATTAGAA
    AATGAAGAGT CTGTGTTGGA AGAAAATGAT GCAGAGAATA ATAAGGAAAC CTACGCATGT
    GATGAAGTTG TGAATACATA TGAAATTAAA AATCCATCAG GCATTTCAAT ATGCACAAGG
    CCTGCAGAGG AAAACGTTGG AAGAAATAGT GTTCAGCAAG TTAATGGAGT CAACATTTCA
    AATTCACCTA ATCAAGCTAA CGAGACTATC AGCAGCTCTA TTGGCGATAT TACCGTTGAC
    TCGGTGCGCG ACCATTTAAA TATGACGACC ACCACTTTGT GTTCTATTAA TACAGAGGAA
    ACCATTGAAA TACGACGGAA ACGAGTACTA GTTCTGTTGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001259787.1
    CDS529..3810
    Translation

    Target ORF information:

    RefSeq Version NM_001272858.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster lethal (2) giant larvae (l(2)gl), transcript variant K, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001272858.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTTAAAGT TTATCAGAGG AAAAGGGCAG CAGCCCAGTG CTGACAGACA CCGCCTACAG 
    AAGGACCTTT TTGCTTATCG TAAGACGGCA CAGCATGGCT TTCCTCATAA GCCTTCGGCT
    CTTGCGTATG ATCCAGTTTT GAAACTTATG GCAATAGGGA CGCAAACAGG GGCTTTAAAA
    GTTTTCGGTC AACCCGGAGT TGAATTGTAC GGTCAGCATA CTTTGTTAAA CAATTCAGCA
    TCGGAGCTTA ATGTACAATT ACTTGAATGG GTGTATGGAA CTGGTCGCAT ACTTTCGTTG
    ACGGCAGCGA ATCAATTAAT TCTATGGGAG CCAGTTGGAG CAACGTTGCT GCCAATCAAA
    ACACTACCGT TTGACGGCAA ACTTAAAAAA GTTTCATCGC TGTGCTGTTC TCTCAGTAAG
    GATCTGCTAT GGATTGGAAC AGAAGGTGGA AACATCTATC AACTGGATTT ACATACATTT
    ACCATTAAGG AGCCTGTAAT TTACCATGAC GTTGTGCTAG AGCAGGTGCC ACCAGCCTAC
    AAGCTAAATC CTGGTGCAAT TGAGTCAATC CGCCAACTTC CAAACTCCCC TAGCAAACTT
    CTAGTTGCAT ACAATCGCGG CCTTTGTGTT TTGTGGGATT TTGAAAGCGC ATCTGTCCAG
    CGAGCATACA TAGCCCCTGG ACATGGACAG AGCGTTGGTC TTACAGTGAA CTTCGAAGGA
    TCTGAATTTA CCTGGTACCA CGCTGATGGT TCATACGCCA CTTGGAGCAT AGATAACCCA
    GAACCGCCGT CGAATGTTAA TTATGTGCCT TATGGACCTG ATCCATGCAA AAGCATAAAT
    CGACTGTACA AAGGCAAGCG AAGATCCAAC GATGTAATTG TTTTTTCCGG CGGCATGCCA
    CGGTCAGCAT ATGGTGATCA CAATTGTGTG TCCGTTCACG CCAGCGATGG ACACAAAGTG
    TGTCTTGACT TTACGTCTAA AGTGATTGAC TTTTTTGTGA CCTTTGAAAA TAATAGAGAT
    GTCGCTGAAG TTCTTGTTGT ACTACTTGAA GAGGAACTCT GCGCTTACGA TCTTACTGAC
    CCTAATATTT GTGCTATCAA AGCGCCATAT CTTCACTCTG TCCATGCATC AGCTGTCACT
    TGCAATTACC TTGCTTCTGA AGTCGTACAG TCGGTATATG AAAGTATTTT AAGAGCTGGA
    GATGAACAAG ACATTGACTA TAGCAATATT AGCTGGCCTA TCACTGGCGG TACTCTCCCG
    GATAACTTAG AAGAATCTGT AGAAGAGGAC GCGACTAAGC TTTATGAGAT TTTGTTAACT
    GGTCACGAAG ATGGTTCTGT TAAATTTTGG GACTGCACTG GAGTGTTGCT TAAACCAATT
    TATAATTTTA AAACTAGCAG CATTTTTGGA AGTGAGTCAG ACTTCCGAGA TGACGCAGCT
    GCAGATATGA GTGCGGAACA AGTCGATGAA GGAGAACCGC CATTTCGGAA ATCAGGACTT
    TTTGATCCTT ATTCAGATGA CCCTCGTTTA GCAGTGAAGA AAATAGCATT CTGCCCAAAA
    ACCGGACAAC TTATTGTTGG TGGCACAGCG GGCCAAATAG TTATAGCCGA CTTCATAGAC
    TTACCCGAAA AAGTGTCTTT AAAATACATT TCAATGAATT TGGTCAGCGA TCGTGATGGA
    TTTGTGTGGA AGGGTCACGA TCAGTTAAAC GTGCGATCGA ACTTATTAGA CGGAGAAGCA
    ATTCCTACGA CGGAACGTGG TGTAAATATA TCGGGAGTAC TGCAAGTTTT GCCGCCAGCC
    AGCATAACAT GCATGGCACT CGAAGCAAGC TGGGGCCTAG TATCTGGTGG GACTGCGCAC
    GGCTTAGTTC TCTTTGACTT CAAAAACTTT GTTCCAGTAT TTCATCGCTG CACTTTAAAC
    CCAAATGATC TTACTGGAGC AGGAGAGCAG CTGTCTCGTC GAAAGTCTTT TAAGAAATCA
    TTGAGGGAGT CATTTAGAAA GCTTCGCAAG GGTCGATCGA CCAGGACCAA CCAGAGCAAT
    CAAGTACCAA CAACGCTGGA AGCAAGACCC GTCGAGAGGC AAATAGAGGC TCGTTGTGCA
    GATGACGGGC TAGGATCCAT GGTGCGATGT TTACTATTTG CCAAAACTTA TGTTACTAAT
    GTCAACATAA CGTCGCCAAC TTTGTGGTCA GCAACAAATG CCAGTACAGT CTCGGTTTTC
    CTTCTGCATT TGCCACCAGC GCAGACCGCG GCAACTGCCG TCCCGTCGGC AAGTGGCAAT
    GCACCACCAC ACATGCCCCG CCGAATTTCT GCGCAGCTTG CTAAAGAAAT ACAATTAAAA
    CATCGTGCTC CTGTGGTGGG TATTTCTATT TTTGATCAGG CGGGTAGCCC TGTCGATCAG
    CTGAACGCCG GTGAAAACGG GAGTCCACCG CATCGTGTAC TTATTGCTTC CGAGGAACAG
    TTCAAGGTGT TTTCACTTCC GCAACTAAAG CCGATTAACA AATATAAGCT TACCGCTAAC
    GAAGGTGCTC GGATTCGCCG CATCCATTTT GGTTCGTTTA GTTGTCGCAT ATCCCCGGAA
    ACACTGCAGA GTATGCACGG TTGTAGCCCA ACTAAGTCCA CGCGTTCACA TGGCGATGGA
    GAGGCGGATC CTAATATCAG TGGAAGCTTG GCTGTAAGTC GTGGAGATGT ATATAACGAA
    ACAGCATTGA TATGTTTAAC GAATATGGGC GATATCATGG TTTTATCAGT ACCTGAATTA
    AAAAGACAGC TGAATGCCGC AGCAGTGCGA CGGGAAGACA TTAATGGAGT TTCGTCACTT
    TGCTTTACAA ACTCTGGAGA AGCACTGTAT ATGATGTCTT CTTCTGAACT GCAGCGTATT
    GCTTTAGCCA CGTCCAGAGT CGTGCAACCC ACTGGCGTTG TTCCAGTAGA ACCATTAGAA
    AATGAAGAGT CTGTGTTGGA AGAAAATGAT GCAGAGAATA ATAAGGAAAC CTACGCATGT
    GATGAAGTTG TGAATACATA TGAAATTAAA AATCCATCAG GCATTTCAAT ATGCACAAGG
    CCTGCAGAGG AAAACGTTGG AAGAAATAGT GTTCAGCAAG TTAATGGAGT CAACATTTCA
    AATTCACCTA ATCAAGCTAA CGAGACTATC AGCAGCTCTA TTGGCGATAT TACCGTTGAC
    TCGGTGCGCG ACCATTTAAA TATGACGACC ACCACTTTGT GTTCTATTAA TACAGAGGAA
    ACCATTGAAA TACGACGGAA ACGAGTACTA GTTCTGTTGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa15843
    Clone ID Related Accession (Same CDS sequence) NM_164352.3 , NM_164350.3 , NM_164351.3
    Accession Version NM_164352.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3339bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product lethal (2) giant larvae, isoform F
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164352.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCAATAG GGACGCAAAC AGGGGCTTTA AAAGTTTTCG GTCAACCCGG AGTTGAATTG 
    TACGGTCAGC ATACTTTGTT AAACAATTCA GCATCGGAGC TTAATGTACA ATTACTTGAA
    TGGGTGTATG GAACTGGTCG CATACTTTCG TTGACGGCAG CGAATCAATT AATTCTATGG
    GAGCCAGTTG GAGCAACGTT GCTGCCAATC AAAACACTAC CGTTTGACGG CAAACTTAAA
    AAAGTTTCAT CGCTGTGCTG TTCTCTCAGT AAGGATCTGC TATGGATTGG AACAGAAGGT
    GGAAACATCT ATCAACTGGA TTTACATACA TTTACCATTA AGGAGCCTGT AATTTACCAT
    GACGTTGTGC TAGAGCAGGT GCCACCAGCC TACAAGCTAA ATCCTGGTGC AATTGAGTCA
    ATCCGCCAAC TTCCAAACTC CCCTAGCAAA CTTCTAGTTG CATACAATCG CGGCCTTTGT
    GTTTTGTGGG ATTTTGAAAG CGCATCTGTC CAGCGAGCAT ACATAGCCCC TGGACATGGA
    CAGAGCGTTG GTCTTACAGT GAACTTCGAA GGATCTGAAT TTACCTGGTA CCACGCTGAT
    GGTTCATACG CCACTTGGAG CATAGATAAC CCAGAACCGC CGTCGAATGT TAATTATGTG
    CCTTATGGAC CTGATCCATG CAAAAGCATA AATCGACTGT ACAAAGGCAA GCGAAGATCC
    AACGATGTAA TTGTTTTTTC CGGCGGCATG CCACGGTCAG CATATGGTGA TCACAATTGT
    GTGTCCGTTC ACGCCAGCGA TGGACACAAA GTGTGTCTTG ACTTTACGTC TAAAGTGATT
    GACTTTTTTG TGACCTTTGA AAATAATAGA GATGTCGCTG AAGTTCTTGT TGTACTACTT
    GAAGAGGAAC TCTGCGCTTA CGATCTTACT GACCCTAATA TTTGTGCTAT CAAAGCGCCA
    TATCTTCACT CTGTCCATGC ATCAGCTGTC ACTTGCAATT ACCTTGCTTC TGAAGTCGTA
    CAGTCGGTAT ATGAAAGTAT TTTAAGAGCT GGAGATGAAC AAGACATTGA CTATAGCAAT
    ATTAGCTGGC CTATCACTGG CGGTACTCTC CCGGATAACT TAGAAGAATC TGTAGAAGAG
    GACGCGACTA AGCTTTATGA GATTTTGTTA ACTGGTCACG AAGATGGTTC TGTTAAATTT
    TGGGACTGCA CTGGAGTGTT GCTTAAACCA ATTTATAATT TTAAAACTAG CAGCATTTTT
    GGAAGTGAGT CAGACTTCCG AGATGACGCA GCTGCAGATA TGAGTGCGGA ACAAGTCGAT
    GAAGGAGAAC CGCCATTTCG GAAATCAGGA CTTTTTGATC CTTATTCAGA TGACCCTCGT
    TTAGCAGTGA AGAAAATAGC ATTCTGCCCA AAAACCGGAC AACTTATTGT TGGTGGCACA
    GCGGGCCAAA TAGTTATAGC CGACTTCATA GACTTACCCG AAAAAGTGTC TTTAAAATAC
    ATTTCAATGA ATTTGGTCAG CGATCGTGAT GGATTTGTGT GGAAGGGTCA CGATCAGTTA
    AACGTGCGAT CGAACTTATT AGACGGAGAA GCAATTCCTA CGACGGAACG TGGTGTAAAT
    ATATCGGGAG TACTGCAAGT TTTGCCGCCA GCCAGCATAA CATGCATGGC ACTCGAAGCA
    AGCTGGGGCC TAGTATCTGG TGGGACTGCG CACGGCTTAG TTCTCTTTGA CTTCAAAAAC
    TTTGTTCCAG TATTTCATCG CTGCACTTTA AACCCAAATG ATCTTACTGG AGCAGGAGAG
    CAGCTGTCTC GTCGAAAGTC TTTTAAGAAA TCATTGAGGG AGTCATTTAG AAAGCTTCGC
    AAGGGTCGAT CGACCAGGAC CAACCAGAGC AATCAAGTAC CAACAACGCT GGAAGCAAGA
    CCCGTCGAGA GGCAAATAGA GGCTCGTTGT GCAGATGACG GGCTAGGATC CATGGTGCGA
    TGTTTACTAT TTGCCAAAAC TTATGTTACT AATGTCAACA TAACGTCGCC AACTTTGTGG
    TCAGCAACAA ATGCCAGTAC AGTCTCGGTT TTCCTTCTGC ATTTGCCACC AGCGCAGACC
    GCGGCAACTG CCGTCCCGTC GGCAAGTGGC AATGCACCAC CACACATGCC CCGCCGAATT
    TCTGCGCAGC TTGCTAAAGA AATACAATTA AAACATCGTG CTCCTGTGGT GGGTATTTCT
    ATTTTTGATC AGGCGGGTAG CCCTGTCGAT CAGCTGAACG CCGGTGAAAA CGGGAGTCCA
    CCGCATCGTG TACTTATTGC TTCCGAGGAA CAGTTCAAGG TGTTTTCACT TCCGCAACTA
    AAGCCGATTA ACAAATATAA GCTTACCGCT AACGAAGGTG CTCGGATTCG CCGCATCCAT
    TTTGGTTCGT TTAGTTGTCG CATATCCCCG GAAACACTGC AGAGTATGCA CGGTTGTAGC
    CCAACTAAGT CCACGCGTTC ACATGGCGAT GGAGAGGCGG ATCCTAATAT CAGTGGAAGC
    TTGGCTGTAA GTCGTGGAGA TGTATATAAC GAAACAGCAT TGATATGTTT AACGAATATG
    GGCGATATCA TGGTTTTATC AGTACCTGAA TTAAAAAGAC AGCTGAATGC CGCAGCAGTG
    CGACGGGAAG ACATTAATGG AGTTTCGTCA CTTTGCTTTA CAAACTCTGG AGAAGCACTG
    TATATGATGT CTTCTTCTGA ACTGCAGCGT ATTGCTTTAG CCACGTCCAG AGTCGTGCAA
    CCCACTGGCG TTGTTCCAGT AGAACCATTA GAAAATGAAG AGTCTGTGTT GGAAGAAAAT
    GATGCAGAGA ATAATAAGGA AACCTACGCA TGTGATGAAG TTGTGAATAC ATATGAAATT
    AAAAATCCAT CAGGCATTTC AATATGCACA AGGCCTGCAG AGGAAAACGT TGGAAGAAAT
    AGTGTTCAGC AAGTTAATGG AGTCAACATT TCAAATTCAC CTAATCAAGC TAACGAGACT
    ATCAGCAGCT CTATTGGCGA TATTACCGTT GACTCGGTGC GCGACCATTT AAATATGACG
    ACCACCACTT TGTGTTCTAT TAATACAGAG GAAACCATTG GTCGCCTATC TGTACTTAGC
    ACGCAAACCA ACAAAGCCAG TACTACCGTA AACATGAGTG AAATTCCAAA TATTAATATT
    TCTAATTTAG AGGACTTGGA ATCGAAAAGA AATACGACGG AAACGAGTAC TAGTTCTGTT
    GTAATTAAAT CTATAATTAC AAACATTTCT CATGAAAAAA CGAACGGAGA CAACAAAATA
    GGAACGCCAA AAACAGCGCC TGAAGAAAGC CAATTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_722587.1
    CDS368..3706
    Translation

    Target ORF information:

    RefSeq Version NM_164352.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster lethal (2) giant larvae (l(2)gl), transcript variant F, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_164352.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCAATAG GGACGCAAAC AGGGGCTTTA AAAGTTTTCG GTCAACCCGG AGTTGAATTG 
    TACGGTCAGC ATACTTTGTT AAACAATTCA GCATCGGAGC TTAATGTACA ATTACTTGAA
    TGGGTGTATG GAACTGGTCG CATACTTTCG TTGACGGCAG CGAATCAATT AATTCTATGG
    GAGCCAGTTG GAGCAACGTT GCTGCCAATC AAAACACTAC CGTTTGACGG CAAACTTAAA
    AAAGTTTCAT CGCTGTGCTG TTCTCTCAGT AAGGATCTGC TATGGATTGG AACAGAAGGT
    GGAAACATCT ATCAACTGGA TTTACATACA TTTACCATTA AGGAGCCTGT AATTTACCAT
    GACGTTGTGC TAGAGCAGGT GCCACCAGCC TACAAGCTAA ATCCTGGTGC AATTGAGTCA
    ATCCGCCAAC TTCCAAACTC CCCTAGCAAA CTTCTAGTTG CATACAATCG CGGCCTTTGT
    GTTTTGTGGG ATTTTGAAAG CGCATCTGTC CAGCGAGCAT ACATAGCCCC TGGACATGGA
    CAGAGCGTTG GTCTTACAGT GAACTTCGAA GGATCTGAAT TTACCTGGTA CCACGCTGAT
    GGTTCATACG CCACTTGGAG CATAGATAAC CCAGAACCGC CGTCGAATGT TAATTATGTG
    CCTTATGGAC CTGATCCATG CAAAAGCATA AATCGACTGT ACAAAGGCAA GCGAAGATCC
    AACGATGTAA TTGTTTTTTC CGGCGGCATG CCACGGTCAG CATATGGTGA TCACAATTGT
    GTGTCCGTTC ACGCCAGCGA TGGACACAAA GTGTGTCTTG ACTTTACGTC TAAAGTGATT
    GACTTTTTTG TGACCTTTGA AAATAATAGA GATGTCGCTG AAGTTCTTGT TGTACTACTT
    GAAGAGGAAC TCTGCGCTTA CGATCTTACT GACCCTAATA TTTGTGCTAT CAAAGCGCCA
    TATCTTCACT CTGTCCATGC ATCAGCTGTC ACTTGCAATT ACCTTGCTTC TGAAGTCGTA
    CAGTCGGTAT ATGAAAGTAT TTTAAGAGCT GGAGATGAAC AAGACATTGA CTATAGCAAT
    ATTAGCTGGC CTATCACTGG CGGTACTCTC CCGGATAACT TAGAAGAATC TGTAGAAGAG
    GACGCGACTA AGCTTTATGA GATTTTGTTA ACTGGTCACG AAGATGGTTC TGTTAAATTT
    TGGGACTGCA CTGGAGTGTT GCTTAAACCA ATTTATAATT TTAAAACTAG CAGCATTTTT
    GGAAGTGAGT CAGACTTCCG AGATGACGCA GCTGCAGATA TGAGTGCGGA ACAAGTCGAT
    GAAGGAGAAC CGCCATTTCG GAAATCAGGA CTTTTTGATC CTTATTCAGA TGACCCTCGT
    TTAGCAGTGA AGAAAATAGC ATTCTGCCCA AAAACCGGAC AACTTATTGT TGGTGGCACA
    GCGGGCCAAA TAGTTATAGC CGACTTCATA GACTTACCCG AAAAAGTGTC TTTAAAATAC
    ATTTCAATGA ATTTGGTCAG CGATCGTGAT GGATTTGTGT GGAAGGGTCA CGATCAGTTA
    AACGTGCGAT CGAACTTATT AGACGGAGAA GCAATTCCTA CGACGGAACG TGGTGTAAAT
    ATATCGGGAG TACTGCAAGT TTTGCCGCCA GCCAGCATAA CATGCATGGC ACTCGAAGCA
    AGCTGGGGCC TAGTATCTGG TGGGACTGCG CACGGCTTAG TTCTCTTTGA CTTCAAAAAC
    TTTGTTCCAG TATTTCATCG CTGCACTTTA AACCCAAATG ATCTTACTGG AGCAGGAGAG
    CAGCTGTCTC GTCGAAAGTC TTTTAAGAAA TCATTGAGGG AGTCATTTAG AAAGCTTCGC
    AAGGGTCGAT CGACCAGGAC CAACCAGAGC AATCAAGTAC CAACAACGCT GGAAGCAAGA
    CCCGTCGAGA GGCAAATAGA GGCTCGTTGT GCAGATGACG GGCTAGGATC CATGGTGCGA
    TGTTTACTAT TTGCCAAAAC TTATGTTACT AATGTCAACA TAACGTCGCC AACTTTGTGG
    TCAGCAACAA ATGCCAGTAC AGTCTCGGTT TTCCTTCTGC ATTTGCCACC AGCGCAGACC
    GCGGCAACTG CCGTCCCGTC GGCAAGTGGC AATGCACCAC CACACATGCC CCGCCGAATT
    TCTGCGCAGC TTGCTAAAGA AATACAATTA AAACATCGTG CTCCTGTGGT GGGTATTTCT
    ATTTTTGATC AGGCGGGTAG CCCTGTCGAT CAGCTGAACG CCGGTGAAAA CGGGAGTCCA
    CCGCATCGTG TACTTATTGC TTCCGAGGAA CAGTTCAAGG TGTTTTCACT TCCGCAACTA
    AAGCCGATTA ACAAATATAA GCTTACCGCT AACGAAGGTG CTCGGATTCG CCGCATCCAT
    TTTGGTTCGT TTAGTTGTCG CATATCCCCG GAAACACTGC AGAGTATGCA CGGTTGTAGC
    CCAACTAAGT CCACGCGTTC ACATGGCGAT GGAGAGGCGG ATCCTAATAT CAGTGGAAGC
    TTGGCTGTAA GTCGTGGAGA TGTATATAAC GAAACAGCAT TGATATGTTT AACGAATATG
    GGCGATATCA TGGTTTTATC AGTACCTGAA TTAAAAAGAC AGCTGAATGC CGCAGCAGTG
    CGACGGGAAG ACATTAATGG AGTTTCGTCA CTTTGCTTTA CAAACTCTGG AGAAGCACTG
    TATATGATGT CTTCTTCTGA ACTGCAGCGT ATTGCTTTAG CCACGTCCAG AGTCGTGCAA
    CCCACTGGCG TTGTTCCAGT AGAACCATTA GAAAATGAAG AGTCTGTGTT GGAAGAAAAT
    GATGCAGAGA ATAATAAGGA AACCTACGCA TGTGATGAAG TTGTGAATAC ATATGAAATT
    AAAAATCCAT CAGGCATTTC AATATGCACA AGGCCTGCAG AGGAAAACGT TGGAAGAAAT
    AGTGTTCAGC AAGTTAATGG AGTCAACATT TCAAATTCAC CTAATCAAGC TAACGAGACT
    ATCAGCAGCT CTATTGGCGA TATTACCGTT GACTCGGTGC GCGACCATTT AAATATGACG
    ACCACCACTT TGTGTTCTAT TAATACAGAG GAAACCATTG GTCGCCTATC TGTACTTAGC
    ACGCAAACCA ACAAAGCCAG TACTACCGTA AACATGAGTG AAATTCCAAA TATTAATATT
    TCTAATTTAG AGGACTTGGA ATCGAAAAGA AATACGACGG AAACGAGTAC TAGTTCTGTT
    GTAATTAAAT CTATAATTAC AAACATTTCT CATGAAAAAA CGAACGGAGA CAACAAAATA
    GGAACGCCAA AAACAGCGCC TGAAGAAAGC CAATTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa15843
    Clone ID Related Accession (Same CDS sequence) NM_164352.3 , NM_164350.3 , NM_164351.3
    Accession Version NM_164350.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3339bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product lethal (2) giant larvae, isoform D
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164350.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCAATAG GGACGCAAAC AGGGGCTTTA AAAGTTTTCG GTCAACCCGG AGTTGAATTG 
    TACGGTCAGC ATACTTTGTT AAACAATTCA GCATCGGAGC TTAATGTACA ATTACTTGAA
    TGGGTGTATG GAACTGGTCG CATACTTTCG TTGACGGCAG CGAATCAATT AATTCTATGG
    GAGCCAGTTG GAGCAACGTT GCTGCCAATC AAAACACTAC CGTTTGACGG CAAACTTAAA
    AAAGTTTCAT CGCTGTGCTG TTCTCTCAGT AAGGATCTGC TATGGATTGG AACAGAAGGT
    GGAAACATCT ATCAACTGGA TTTACATACA TTTACCATTA AGGAGCCTGT AATTTACCAT
    GACGTTGTGC TAGAGCAGGT GCCACCAGCC TACAAGCTAA ATCCTGGTGC AATTGAGTCA
    ATCCGCCAAC TTCCAAACTC CCCTAGCAAA CTTCTAGTTG CATACAATCG CGGCCTTTGT
    GTTTTGTGGG ATTTTGAAAG CGCATCTGTC CAGCGAGCAT ACATAGCCCC TGGACATGGA
    CAGAGCGTTG GTCTTACAGT GAACTTCGAA GGATCTGAAT TTACCTGGTA CCACGCTGAT
    GGTTCATACG CCACTTGGAG CATAGATAAC CCAGAACCGC CGTCGAATGT TAATTATGTG
    CCTTATGGAC CTGATCCATG CAAAAGCATA AATCGACTGT ACAAAGGCAA GCGAAGATCC
    AACGATGTAA TTGTTTTTTC CGGCGGCATG CCACGGTCAG CATATGGTGA TCACAATTGT
    GTGTCCGTTC ACGCCAGCGA TGGACACAAA GTGTGTCTTG ACTTTACGTC TAAAGTGATT
    GACTTTTTTG TGACCTTTGA AAATAATAGA GATGTCGCTG AAGTTCTTGT TGTACTACTT
    GAAGAGGAAC TCTGCGCTTA CGATCTTACT GACCCTAATA TTTGTGCTAT CAAAGCGCCA
    TATCTTCACT CTGTCCATGC ATCAGCTGTC ACTTGCAATT ACCTTGCTTC TGAAGTCGTA
    CAGTCGGTAT ATGAAAGTAT TTTAAGAGCT GGAGATGAAC AAGACATTGA CTATAGCAAT
    ATTAGCTGGC CTATCACTGG CGGTACTCTC CCGGATAACT TAGAAGAATC TGTAGAAGAG
    GACGCGACTA AGCTTTATGA GATTTTGTTA ACTGGTCACG AAGATGGTTC TGTTAAATTT
    TGGGACTGCA CTGGAGTGTT GCTTAAACCA ATTTATAATT TTAAAACTAG CAGCATTTTT
    GGAAGTGAGT CAGACTTCCG AGATGACGCA GCTGCAGATA TGAGTGCGGA ACAAGTCGAT
    GAAGGAGAAC CGCCATTTCG GAAATCAGGA CTTTTTGATC CTTATTCAGA TGACCCTCGT
    TTAGCAGTGA AGAAAATAGC ATTCTGCCCA AAAACCGGAC AACTTATTGT TGGTGGCACA
    GCGGGCCAAA TAGTTATAGC CGACTTCATA GACTTACCCG AAAAAGTGTC TTTAAAATAC
    ATTTCAATGA ATTTGGTCAG CGATCGTGAT GGATTTGTGT GGAAGGGTCA CGATCAGTTA
    AACGTGCGAT CGAACTTATT AGACGGAGAA GCAATTCCTA CGACGGAACG TGGTGTAAAT
    ATATCGGGAG TACTGCAAGT TTTGCCGCCA GCCAGCATAA CATGCATGGC ACTCGAAGCA
    AGCTGGGGCC TAGTATCTGG TGGGACTGCG CACGGCTTAG TTCTCTTTGA CTTCAAAAAC
    TTTGTTCCAG TATTTCATCG CTGCACTTTA AACCCAAATG ATCTTACTGG AGCAGGAGAG
    CAGCTGTCTC GTCGAAAGTC TTTTAAGAAA TCATTGAGGG AGTCATTTAG AAAGCTTCGC
    AAGGGTCGAT CGACCAGGAC CAACCAGAGC AATCAAGTAC CAACAACGCT GGAAGCAAGA
    CCCGTCGAGA GGCAAATAGA GGCTCGTTGT GCAGATGACG GGCTAGGATC CATGGTGCGA
    TGTTTACTAT TTGCCAAAAC TTATGTTACT AATGTCAACA TAACGTCGCC AACTTTGTGG
    TCAGCAACAA ATGCCAGTAC AGTCTCGGTT TTCCTTCTGC ATTTGCCACC AGCGCAGACC
    GCGGCAACTG CCGTCCCGTC GGCAAGTGGC AATGCACCAC CACACATGCC CCGCCGAATT
    TCTGCGCAGC TTGCTAAAGA AATACAATTA AAACATCGTG CTCCTGTGGT GGGTATTTCT
    ATTTTTGATC AGGCGGGTAG CCCTGTCGAT CAGCTGAACG CCGGTGAAAA CGGGAGTCCA
    CCGCATCGTG TACTTATTGC TTCCGAGGAA CAGTTCAAGG TGTTTTCACT TCCGCAACTA
    AAGCCGATTA ACAAATATAA GCTTACCGCT AACGAAGGTG CTCGGATTCG CCGCATCCAT
    TTTGGTTCGT TTAGTTGTCG CATATCCCCG GAAACACTGC AGAGTATGCA CGGTTGTAGC
    CCAACTAAGT CCACGCGTTC ACATGGCGAT GGAGAGGCGG ATCCTAATAT CAGTGGAAGC
    TTGGCTGTAA GTCGTGGAGA TGTATATAAC GAAACAGCAT TGATATGTTT AACGAATATG
    GGCGATATCA TGGTTTTATC AGTACCTGAA TTAAAAAGAC AGCTGAATGC CGCAGCAGTG
    CGACGGGAAG ACATTAATGG AGTTTCGTCA CTTTGCTTTA CAAACTCTGG AGAAGCACTG
    TATATGATGT CTTCTTCTGA ACTGCAGCGT ATTGCTTTAG CCACGTCCAG AGTCGTGCAA
    CCCACTGGCG TTGTTCCAGT AGAACCATTA GAAAATGAAG AGTCTGTGTT GGAAGAAAAT
    GATGCAGAGA ATAATAAGGA AACCTACGCA TGTGATGAAG TTGTGAATAC ATATGAAATT
    AAAAATCCAT CAGGCATTTC AATATGCACA AGGCCTGCAG AGGAAAACGT TGGAAGAAAT
    AGTGTTCAGC AAGTTAATGG AGTCAACATT TCAAATTCAC CTAATCAAGC TAACGAGACT
    ATCAGCAGCT CTATTGGCGA TATTACCGTT GACTCGGTGC GCGACCATTT AAATATGACG
    ACCACCACTT TGTGTTCTAT TAATACAGAG GAAACCATTG GTCGCCTATC TGTACTTAGC
    ACGCAAACCA ACAAAGCCAG TACTACCGTA AACATGAGTG AAATTCCAAA TATTAATATT
    TCTAATTTAG AGGACTTGGA ATCGAAAAGA AATACGACGG AAACGAGTAC TAGTTCTGTT
    GTAATTAAAT CTATAATTAC AAACATTTCT CATGAAAAAA CGAACGGAGA CAACAAAATA
    GGAACGCCAA AAACAGCGCC TGAAGAAAGC CAATTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_722585.1
    CDS516..3854
    Translation

    Target ORF information:

    RefSeq Version NM_164350.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster lethal (2) giant larvae (l(2)gl), transcript variant D, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_164350.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCAATAG GGACGCAAAC AGGGGCTTTA AAAGTTTTCG GTCAACCCGG AGTTGAATTG 
    TACGGTCAGC ATACTTTGTT AAACAATTCA GCATCGGAGC TTAATGTACA ATTACTTGAA
    TGGGTGTATG GAACTGGTCG CATACTTTCG TTGACGGCAG CGAATCAATT AATTCTATGG
    GAGCCAGTTG GAGCAACGTT GCTGCCAATC AAAACACTAC CGTTTGACGG CAAACTTAAA
    AAAGTTTCAT CGCTGTGCTG TTCTCTCAGT AAGGATCTGC TATGGATTGG AACAGAAGGT
    GGAAACATCT ATCAACTGGA TTTACATACA TTTACCATTA AGGAGCCTGT AATTTACCAT
    GACGTTGTGC TAGAGCAGGT GCCACCAGCC TACAAGCTAA ATCCTGGTGC AATTGAGTCA
    ATCCGCCAAC TTCCAAACTC CCCTAGCAAA CTTCTAGTTG CATACAATCG CGGCCTTTGT
    GTTTTGTGGG ATTTTGAAAG CGCATCTGTC CAGCGAGCAT ACATAGCCCC TGGACATGGA
    CAGAGCGTTG GTCTTACAGT GAACTTCGAA GGATCTGAAT TTACCTGGTA CCACGCTGAT
    GGTTCATACG CCACTTGGAG CATAGATAAC CCAGAACCGC CGTCGAATGT TAATTATGTG
    CCTTATGGAC CTGATCCATG CAAAAGCATA AATCGACTGT ACAAAGGCAA GCGAAGATCC
    AACGATGTAA TTGTTTTTTC CGGCGGCATG CCACGGTCAG CATATGGTGA TCACAATTGT
    GTGTCCGTTC ACGCCAGCGA TGGACACAAA GTGTGTCTTG ACTTTACGTC TAAAGTGATT
    GACTTTTTTG TGACCTTTGA AAATAATAGA GATGTCGCTG AAGTTCTTGT TGTACTACTT
    GAAGAGGAAC TCTGCGCTTA CGATCTTACT GACCCTAATA TTTGTGCTAT CAAAGCGCCA
    TATCTTCACT CTGTCCATGC ATCAGCTGTC ACTTGCAATT ACCTTGCTTC TGAAGTCGTA
    CAGTCGGTAT ATGAAAGTAT TTTAAGAGCT GGAGATGAAC AAGACATTGA CTATAGCAAT
    ATTAGCTGGC CTATCACTGG CGGTACTCTC CCGGATAACT TAGAAGAATC TGTAGAAGAG
    GACGCGACTA AGCTTTATGA GATTTTGTTA ACTGGTCACG AAGATGGTTC TGTTAAATTT
    TGGGACTGCA CTGGAGTGTT GCTTAAACCA ATTTATAATT TTAAAACTAG CAGCATTTTT
    GGAAGTGAGT CAGACTTCCG AGATGACGCA GCTGCAGATA TGAGTGCGGA ACAAGTCGAT
    GAAGGAGAAC CGCCATTTCG GAAATCAGGA CTTTTTGATC CTTATTCAGA TGACCCTCGT
    TTAGCAGTGA AGAAAATAGC ATTCTGCCCA AAAACCGGAC AACTTATTGT TGGTGGCACA
    GCGGGCCAAA TAGTTATAGC CGACTTCATA GACTTACCCG AAAAAGTGTC TTTAAAATAC
    ATTTCAATGA ATTTGGTCAG CGATCGTGAT GGATTTGTGT GGAAGGGTCA CGATCAGTTA
    AACGTGCGAT CGAACTTATT AGACGGAGAA GCAATTCCTA CGACGGAACG TGGTGTAAAT
    ATATCGGGAG TACTGCAAGT TTTGCCGCCA GCCAGCATAA CATGCATGGC ACTCGAAGCA
    AGCTGGGGCC TAGTATCTGG TGGGACTGCG CACGGCTTAG TTCTCTTTGA CTTCAAAAAC
    TTTGTTCCAG TATTTCATCG CTGCACTTTA AACCCAAATG ATCTTACTGG AGCAGGAGAG
    CAGCTGTCTC GTCGAAAGTC TTTTAAGAAA TCATTGAGGG AGTCATTTAG AAAGCTTCGC
    AAGGGTCGAT CGACCAGGAC CAACCAGAGC AATCAAGTAC CAACAACGCT GGAAGCAAGA
    CCCGTCGAGA GGCAAATAGA GGCTCGTTGT GCAGATGACG GGCTAGGATC CATGGTGCGA
    TGTTTACTAT TTGCCAAAAC TTATGTTACT AATGTCAACA TAACGTCGCC AACTTTGTGG
    TCAGCAACAA ATGCCAGTAC AGTCTCGGTT TTCCTTCTGC ATTTGCCACC AGCGCAGACC
    GCGGCAACTG CCGTCCCGTC GGCAAGTGGC AATGCACCAC CACACATGCC CCGCCGAATT
    TCTGCGCAGC TTGCTAAAGA AATACAATTA AAACATCGTG CTCCTGTGGT GGGTATTTCT
    ATTTTTGATC AGGCGGGTAG CCCTGTCGAT CAGCTGAACG CCGGTGAAAA CGGGAGTCCA
    CCGCATCGTG TACTTATTGC TTCCGAGGAA CAGTTCAAGG TGTTTTCACT TCCGCAACTA
    AAGCCGATTA ACAAATATAA GCTTACCGCT AACGAAGGTG CTCGGATTCG CCGCATCCAT
    TTTGGTTCGT TTAGTTGTCG CATATCCCCG GAAACACTGC AGAGTATGCA CGGTTGTAGC
    CCAACTAAGT CCACGCGTTC ACATGGCGAT GGAGAGGCGG ATCCTAATAT CAGTGGAAGC
    TTGGCTGTAA GTCGTGGAGA TGTATATAAC GAAACAGCAT TGATATGTTT AACGAATATG
    GGCGATATCA TGGTTTTATC AGTACCTGAA TTAAAAAGAC AGCTGAATGC CGCAGCAGTG
    CGACGGGAAG ACATTAATGG AGTTTCGTCA CTTTGCTTTA CAAACTCTGG AGAAGCACTG
    TATATGATGT CTTCTTCTGA ACTGCAGCGT ATTGCTTTAG CCACGTCCAG AGTCGTGCAA
    CCCACTGGCG TTGTTCCAGT AGAACCATTA GAAAATGAAG AGTCTGTGTT GGAAGAAAAT
    GATGCAGAGA ATAATAAGGA AACCTACGCA TGTGATGAAG TTGTGAATAC ATATGAAATT
    AAAAATCCAT CAGGCATTTC AATATGCACA AGGCCTGCAG AGGAAAACGT TGGAAGAAAT
    AGTGTTCAGC AAGTTAATGG AGTCAACATT TCAAATTCAC CTAATCAAGC TAACGAGACT
    ATCAGCAGCT CTATTGGCGA TATTACCGTT GACTCGGTGC GCGACCATTT AAATATGACG
    ACCACCACTT TGTGTTCTAT TAATACAGAG GAAACCATTG GTCGCCTATC TGTACTTAGC
    ACGCAAACCA ACAAAGCCAG TACTACCGTA AACATGAGTG AAATTCCAAA TATTAATATT
    TCTAATTTAG AGGACTTGGA ATCGAAAAGA AATACGACGG AAACGAGTAC TAGTTCTGTT
    GTAATTAAAT CTATAATTAC AAACATTTCT CATGAAAAAA CGAACGGAGA CAACAAAATA
    GGAACGCCAA AAACAGCGCC TGAAGAAAGC CAATTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa15843
    Clone ID Related Accession (Same CDS sequence) NM_164352.3 , NM_164350.3 , NM_164351.3
    Accession Version NM_164351.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3339bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product lethal (2) giant larvae, isoform E
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164351.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCAATAG GGACGCAAAC AGGGGCTTTA AAAGTTTTCG GTCAACCCGG AGTTGAATTG 
    TACGGTCAGC ATACTTTGTT AAACAATTCA GCATCGGAGC TTAATGTACA ATTACTTGAA
    TGGGTGTATG GAACTGGTCG CATACTTTCG TTGACGGCAG CGAATCAATT AATTCTATGG
    GAGCCAGTTG GAGCAACGTT GCTGCCAATC AAAACACTAC CGTTTGACGG CAAACTTAAA
    AAAGTTTCAT CGCTGTGCTG TTCTCTCAGT AAGGATCTGC TATGGATTGG AACAGAAGGT
    GGAAACATCT ATCAACTGGA TTTACATACA TTTACCATTA AGGAGCCTGT AATTTACCAT
    GACGTTGTGC TAGAGCAGGT GCCACCAGCC TACAAGCTAA ATCCTGGTGC AATTGAGTCA
    ATCCGCCAAC TTCCAAACTC CCCTAGCAAA CTTCTAGTTG CATACAATCG CGGCCTTTGT
    GTTTTGTGGG ATTTTGAAAG CGCATCTGTC CAGCGAGCAT ACATAGCCCC TGGACATGGA
    CAGAGCGTTG GTCTTACAGT GAACTTCGAA GGATCTGAAT TTACCTGGTA CCACGCTGAT
    GGTTCATACG CCACTTGGAG CATAGATAAC CCAGAACCGC CGTCGAATGT TAATTATGTG
    CCTTATGGAC CTGATCCATG CAAAAGCATA AATCGACTGT ACAAAGGCAA GCGAAGATCC
    AACGATGTAA TTGTTTTTTC CGGCGGCATG CCACGGTCAG CATATGGTGA TCACAATTGT
    GTGTCCGTTC ACGCCAGCGA TGGACACAAA GTGTGTCTTG ACTTTACGTC TAAAGTGATT
    GACTTTTTTG TGACCTTTGA AAATAATAGA GATGTCGCTG AAGTTCTTGT TGTACTACTT
    GAAGAGGAAC TCTGCGCTTA CGATCTTACT GACCCTAATA TTTGTGCTAT CAAAGCGCCA
    TATCTTCACT CTGTCCATGC ATCAGCTGTC ACTTGCAATT ACCTTGCTTC TGAAGTCGTA
    CAGTCGGTAT ATGAAAGTAT TTTAAGAGCT GGAGATGAAC AAGACATTGA CTATAGCAAT
    ATTAGCTGGC CTATCACTGG CGGTACTCTC CCGGATAACT TAGAAGAATC TGTAGAAGAG
    GACGCGACTA AGCTTTATGA GATTTTGTTA ACTGGTCACG AAGATGGTTC TGTTAAATTT
    TGGGACTGCA CTGGAGTGTT GCTTAAACCA ATTTATAATT TTAAAACTAG CAGCATTTTT
    GGAAGTGAGT CAGACTTCCG AGATGACGCA GCTGCAGATA TGAGTGCGGA ACAAGTCGAT
    GAAGGAGAAC CGCCATTTCG GAAATCAGGA CTTTTTGATC CTTATTCAGA TGACCCTCGT
    TTAGCAGTGA AGAAAATAGC ATTCTGCCCA AAAACCGGAC AACTTATTGT TGGTGGCACA
    GCGGGCCAAA TAGTTATAGC CGACTTCATA GACTTACCCG AAAAAGTGTC TTTAAAATAC
    ATTTCAATGA ATTTGGTCAG CGATCGTGAT GGATTTGTGT GGAAGGGTCA CGATCAGTTA
    AACGTGCGAT CGAACTTATT AGACGGAGAA GCAATTCCTA CGACGGAACG TGGTGTAAAT
    ATATCGGGAG TACTGCAAGT TTTGCCGCCA GCCAGCATAA CATGCATGGC ACTCGAAGCA
    AGCTGGGGCC TAGTATCTGG TGGGACTGCG CACGGCTTAG TTCTCTTTGA CTTCAAAAAC
    TTTGTTCCAG TATTTCATCG CTGCACTTTA AACCCAAATG ATCTTACTGG AGCAGGAGAG
    CAGCTGTCTC GTCGAAAGTC TTTTAAGAAA TCATTGAGGG AGTCATTTAG AAAGCTTCGC
    AAGGGTCGAT CGACCAGGAC CAACCAGAGC AATCAAGTAC CAACAACGCT GGAAGCAAGA
    CCCGTCGAGA GGCAAATAGA GGCTCGTTGT GCAGATGACG GGCTAGGATC CATGGTGCGA
    TGTTTACTAT TTGCCAAAAC TTATGTTACT AATGTCAACA TAACGTCGCC AACTTTGTGG
    TCAGCAACAA ATGCCAGTAC AGTCTCGGTT TTCCTTCTGC ATTTGCCACC AGCGCAGACC
    GCGGCAACTG CCGTCCCGTC GGCAAGTGGC AATGCACCAC CACACATGCC CCGCCGAATT
    TCTGCGCAGC TTGCTAAAGA AATACAATTA AAACATCGTG CTCCTGTGGT GGGTATTTCT
    ATTTTTGATC AGGCGGGTAG CCCTGTCGAT CAGCTGAACG CCGGTGAAAA CGGGAGTCCA
    CCGCATCGTG TACTTATTGC TTCCGAGGAA CAGTTCAAGG TGTTTTCACT TCCGCAACTA
    AAGCCGATTA ACAAATATAA GCTTACCGCT AACGAAGGTG CTCGGATTCG CCGCATCCAT
    TTTGGTTCGT TTAGTTGTCG CATATCCCCG GAAACACTGC AGAGTATGCA CGGTTGTAGC
    CCAACTAAGT CCACGCGTTC ACATGGCGAT GGAGAGGCGG ATCCTAATAT CAGTGGAAGC
    TTGGCTGTAA GTCGTGGAGA TGTATATAAC GAAACAGCAT TGATATGTTT AACGAATATG
    GGCGATATCA TGGTTTTATC AGTACCTGAA TTAAAAAGAC AGCTGAATGC CGCAGCAGTG
    CGACGGGAAG ACATTAATGG AGTTTCGTCA CTTTGCTTTA CAAACTCTGG AGAAGCACTG
    TATATGATGT CTTCTTCTGA ACTGCAGCGT ATTGCTTTAG CCACGTCCAG AGTCGTGCAA
    CCCACTGGCG TTGTTCCAGT AGAACCATTA GAAAATGAAG AGTCTGTGTT GGAAGAAAAT
    GATGCAGAGA ATAATAAGGA AACCTACGCA TGTGATGAAG TTGTGAATAC ATATGAAATT
    AAAAATCCAT CAGGCATTTC AATATGCACA AGGCCTGCAG AGGAAAACGT TGGAAGAAAT
    AGTGTTCAGC AAGTTAATGG AGTCAACATT TCAAATTCAC CTAATCAAGC TAACGAGACT
    ATCAGCAGCT CTATTGGCGA TATTACCGTT GACTCGGTGC GCGACCATTT AAATATGACG
    ACCACCACTT TGTGTTCTAT TAATACAGAG GAAACCATTG GTCGCCTATC TGTACTTAGC
    ACGCAAACCA ACAAAGCCAG TACTACCGTA AACATGAGTG AAATTCCAAA TATTAATATT
    TCTAATTTAG AGGACTTGGA ATCGAAAAGA AATACGACGG AAACGAGTAC TAGTTCTGTT
    GTAATTAAAT CTATAATTAC AAACATTTCT CATGAAAAAA CGAACGGAGA CAACAAAATA
    GGAACGCCAA AAACAGCGCC TGAAGAAAGC CAATTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_722586.1
    CDS511..3849
    Translation

    Target ORF information:

    RefSeq Version NM_164351.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster lethal (2) giant larvae (l(2)gl), transcript variant E, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_164351.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCAATAG GGACGCAAAC AGGGGCTTTA AAAGTTTTCG GTCAACCCGG AGTTGAATTG 
    TACGGTCAGC ATACTTTGTT AAACAATTCA GCATCGGAGC TTAATGTACA ATTACTTGAA
    TGGGTGTATG GAACTGGTCG CATACTTTCG TTGACGGCAG CGAATCAATT AATTCTATGG
    GAGCCAGTTG GAGCAACGTT GCTGCCAATC AAAACACTAC CGTTTGACGG CAAACTTAAA
    AAAGTTTCAT CGCTGTGCTG TTCTCTCAGT AAGGATCTGC TATGGATTGG AACAGAAGGT
    GGAAACATCT ATCAACTGGA TTTACATACA TTTACCATTA AGGAGCCTGT AATTTACCAT
    GACGTTGTGC TAGAGCAGGT GCCACCAGCC TACAAGCTAA ATCCTGGTGC AATTGAGTCA
    ATCCGCCAAC TTCCAAACTC CCCTAGCAAA CTTCTAGTTG CATACAATCG CGGCCTTTGT
    GTTTTGTGGG ATTTTGAAAG CGCATCTGTC CAGCGAGCAT ACATAGCCCC TGGACATGGA
    CAGAGCGTTG GTCTTACAGT GAACTTCGAA GGATCTGAAT TTACCTGGTA CCACGCTGAT
    GGTTCATACG CCACTTGGAG CATAGATAAC CCAGAACCGC CGTCGAATGT TAATTATGTG
    CCTTATGGAC CTGATCCATG CAAAAGCATA AATCGACTGT ACAAAGGCAA GCGAAGATCC
    AACGATGTAA TTGTTTTTTC CGGCGGCATG CCACGGTCAG CATATGGTGA TCACAATTGT
    GTGTCCGTTC ACGCCAGCGA TGGACACAAA GTGTGTCTTG ACTTTACGTC TAAAGTGATT
    GACTTTTTTG TGACCTTTGA AAATAATAGA GATGTCGCTG AAGTTCTTGT TGTACTACTT
    GAAGAGGAAC TCTGCGCTTA CGATCTTACT GACCCTAATA TTTGTGCTAT CAAAGCGCCA
    TATCTTCACT CTGTCCATGC ATCAGCTGTC ACTTGCAATT ACCTTGCTTC TGAAGTCGTA
    CAGTCGGTAT ATGAAAGTAT TTTAAGAGCT GGAGATGAAC AAGACATTGA CTATAGCAAT
    ATTAGCTGGC CTATCACTGG CGGTACTCTC CCGGATAACT TAGAAGAATC TGTAGAAGAG
    GACGCGACTA AGCTTTATGA GATTTTGTTA ACTGGTCACG AAGATGGTTC TGTTAAATTT
    TGGGACTGCA CTGGAGTGTT GCTTAAACCA ATTTATAATT TTAAAACTAG CAGCATTTTT
    GGAAGTGAGT CAGACTTCCG AGATGACGCA GCTGCAGATA TGAGTGCGGA ACAAGTCGAT
    GAAGGAGAAC CGCCATTTCG GAAATCAGGA CTTTTTGATC CTTATTCAGA TGACCCTCGT
    TTAGCAGTGA AGAAAATAGC ATTCTGCCCA AAAACCGGAC AACTTATTGT TGGTGGCACA
    GCGGGCCAAA TAGTTATAGC CGACTTCATA GACTTACCCG AAAAAGTGTC TTTAAAATAC
    ATTTCAATGA ATTTGGTCAG CGATCGTGAT GGATTTGTGT GGAAGGGTCA CGATCAGTTA
    AACGTGCGAT CGAACTTATT AGACGGAGAA GCAATTCCTA CGACGGAACG TGGTGTAAAT
    ATATCGGGAG TACTGCAAGT TTTGCCGCCA GCCAGCATAA CATGCATGGC ACTCGAAGCA
    AGCTGGGGCC TAGTATCTGG TGGGACTGCG CACGGCTTAG TTCTCTTTGA CTTCAAAAAC
    TTTGTTCCAG TATTTCATCG CTGCACTTTA AACCCAAATG ATCTTACTGG AGCAGGAGAG
    CAGCTGTCTC GTCGAAAGTC TTTTAAGAAA TCATTGAGGG AGTCATTTAG AAAGCTTCGC
    AAGGGTCGAT CGACCAGGAC CAACCAGAGC AATCAAGTAC CAACAACGCT GGAAGCAAGA
    CCCGTCGAGA GGCAAATAGA GGCTCGTTGT GCAGATGACG GGCTAGGATC CATGGTGCGA
    TGTTTACTAT TTGCCAAAAC TTATGTTACT AATGTCAACA TAACGTCGCC AACTTTGTGG
    TCAGCAACAA ATGCCAGTAC AGTCTCGGTT TTCCTTCTGC ATTTGCCACC AGCGCAGACC
    GCGGCAACTG CCGTCCCGTC GGCAAGTGGC AATGCACCAC CACACATGCC CCGCCGAATT
    TCTGCGCAGC TTGCTAAAGA AATACAATTA AAACATCGTG CTCCTGTGGT GGGTATTTCT
    ATTTTTGATC AGGCGGGTAG CCCTGTCGAT CAGCTGAACG CCGGTGAAAA CGGGAGTCCA
    CCGCATCGTG TACTTATTGC TTCCGAGGAA CAGTTCAAGG TGTTTTCACT TCCGCAACTA
    AAGCCGATTA ACAAATATAA GCTTACCGCT AACGAAGGTG CTCGGATTCG CCGCATCCAT
    TTTGGTTCGT TTAGTTGTCG CATATCCCCG GAAACACTGC AGAGTATGCA CGGTTGTAGC
    CCAACTAAGT CCACGCGTTC ACATGGCGAT GGAGAGGCGG ATCCTAATAT CAGTGGAAGC
    TTGGCTGTAA GTCGTGGAGA TGTATATAAC GAAACAGCAT TGATATGTTT AACGAATATG
    GGCGATATCA TGGTTTTATC AGTACCTGAA TTAAAAAGAC AGCTGAATGC CGCAGCAGTG
    CGACGGGAAG ACATTAATGG AGTTTCGTCA CTTTGCTTTA CAAACTCTGG AGAAGCACTG
    TATATGATGT CTTCTTCTGA ACTGCAGCGT ATTGCTTTAG CCACGTCCAG AGTCGTGCAA
    CCCACTGGCG TTGTTCCAGT AGAACCATTA GAAAATGAAG AGTCTGTGTT GGAAGAAAAT
    GATGCAGAGA ATAATAAGGA AACCTACGCA TGTGATGAAG TTGTGAATAC ATATGAAATT
    AAAAATCCAT CAGGCATTTC AATATGCACA AGGCCTGCAG AGGAAAACGT TGGAAGAAAT
    AGTGTTCAGC AAGTTAATGG AGTCAACATT TCAAATTCAC CTAATCAAGC TAACGAGACT
    ATCAGCAGCT CTATTGGCGA TATTACCGTT GACTCGGTGC GCGACCATTT AAATATGACG
    ACCACCACTT TGTGTTCTAT TAATACAGAG GAAACCATTG GTCGCCTATC TGTACTTAGC
    ACGCAAACCA ACAAAGCCAG TACTACCGTA AACATGAGTG AAATTCCAAA TATTAATATT
    TCTAATTTAG AGGACTTGGA ATCGAAAAGA AATACGACGG AAACGAGTAC TAGTTCTGTT
    GTAATTAAAT CTATAATTAC AAACATTTCT CATGAAAAAA CGAACGGAGA CAACAAAATA
    GGAACGCCAA AAACAGCGCC TGAAGAAAGC CAATTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa15852
    Clone ID Related Accession (Same CDS sequence) NM_164348.3
    Accession Version NM_164348.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3462bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product lethal (2) giant larvae, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164348.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTTAAAGT TTATCAGAGG AAAAGGGCAG CAGCCCAGTG CTGACAGACA ACGCTACATC 
    ACGGCACAGC ATGGCTTTCC TCATAAGCCT TCGGCTCTTG CGTATGATCC AGTTTTGAAA
    CTTATGGCAA TAGGGACGCA AACAGGGGCT TTAAAAGTTT TCGGTCAACC CGGAGTTGAA
    TTGTACGGTC AGCATACTTT GTTAAACAAT TCAGCATCGG AGCTTAATGT ACAATTACTT
    GAATGGGTGT ATGGAACTGG TCGCATACTT TCGTTGACGG CAGCGAATCA ATTAATTCTA
    TGGGAGCCAG TTGGAGCAAC GTTGCTGCCA ATCAAAACAC TACCGTTTGA CGGCAAACTT
    AAAAAAGTTT CATCGCTGTG CTGTTCTCTC AGTAAGGATC TGCTATGGAT TGGAACAGAA
    GGTGGAAACA TCTATCAACT GGATTTACAT ACATTTACCA TTAAGGAGCC TGTAATTTAC
    CATGACGTTG TGCTAGAGCA GGTGCCACCA GCCTACAAGC TAAATCCTGG TGCAATTGAG
    TCAATCCGCC AACTTCCAAA CTCCCCTAGC AAACTTCTAG TTGCATACAA TCGCGGCCTT
    TGTGTTTTGT GGGATTTTGA AAGCGCATCT GTCCAGCGAG CATACATAGC CCCTGGACAT
    GGACAGAGCG TTGGTCTTAC AGTGAACTTC GAAGGATCTG AATTTACCTG GTACCACGCT
    GATGGTTCAT ACGCCACTTG GAGCATAGAT AACCCAGAAC CGCCGTCGAA TGTTAATTAT
    GTGCCTTATG GACCTGATCC ATGCAAAAGC ATAAATCGAC TGTACAAAGG CAAGCGAAGA
    TCCAACGATG TAATTGTTTT TTCCGGCGGC ATGCCACGGT CAGCATATGG TGATCACAAT
    TGTGTGTCCG TTCACGCCAG CGATGGACAC AAAGTGTGTC TTGACTTTAC GTCTAAAGTG
    ATTGACTTTT TTGTGACCTT TGAAAATAAT AGAGATGTCG CTGAAGTTCT TGTTGTACTA
    CTTGAAGAGG AACTCTGCGC TTACGATCTT ACTGACCCTA ATATTTGTGC TATCAAAGCG
    CCATATCTTC ACTCTGTCCA TGCATCAGCT GTCACTTGCA ATTACCTTGC TTCTGAAGTC
    GTACAGTCGG TATATGAAAG TATTTTAAGA GCTGGAGATG AACAAGACAT TGACTATAGC
    AATATTAGCT GGCCTATCAC TGGCGGTACT CTCCCGGATA ACTTAGAAGA ATCTGTAGAA
    GAGGACGCGA CTAAGCTTTA TGAGATTTTG TTAACTGGTC ACGAAGATGG TTCTGTTAAA
    TTTTGGGACT GCACTGGAGT GTTGCTTAAA CCAATTTATA ATTTTAAAAC TAGCAGCATT
    TTTGGAAGTG AGTCAGACTT CCGAGATGAC GCAGCTGCAG ATATGAGTGC GGAACAAGTC
    GATGAAGGAG AACCGCCATT TCGGAAATCA GGACTTTTTG ATCCTTATTC AGATGACCCT
    CGTTTAGCAG TGAAGAAAAT AGCATTCTGC CCAAAAACCG GACAACTTAT TGTTGGTGGC
    ACAGCGGGCC AAATAGTTAT AGCCGACTTC ATAGACTTAC CCGAAAAAGT GTCTTTAAAA
    TACATTTCAA TGAATTTGGT CAGCGATCGT GATGGATTTG TGTGGAAGGG TCACGATCAG
    TTAAACGTGC GATCGAACTT ATTAGACGGA GAAGCAATTC CTACGACGGA ACGTGGTGTA
    AATATATCGG GAGTACTGCA AGTTTTGCCG CCAGCCAGCA TAACATGCAT GGCACTCGAA
    GCAAGCTGGG GCCTAGTATC TGGTGGGACT GCGCACGGCT TAGTTCTCTT TGACTTCAAA
    AACTTTGTTC CAGTATTTCA TCGCTGCACT TTAAACCCAA ATGATCTTAC TGGAGCAGGA
    GAGCAGCTGT CTCGTCGAAA GTCTTTTAAG AAATCATTGA GGGAGTCATT TAGAAAGCTT
    CGCAAGGGTC GATCGACCAG GACCAACCAG AGCAATCAAG TACCAACAAC GCTGGAAGCA
    AGACCCGTCG AGAGGCAAAT AGAGGCTCGT TGTGCAGATG ACGGGCTAGG ATCCATGGTG
    CGATGTTTAC TATTTGCCAA AACTTATGTT ACTAATGTCA ACATAACGTC GCCAACTTTG
    TGGTCAGCAA CAAATGCCAG TACAGTCTCG GTTTTCCTTC TGCATTTGCC ACCAGCGCAG
    ACCGCGGCAA CTGCCGTCCC GTCGGCAAGT GGCAATGCAC CACCACACAT GCCCCGCCGA
    ATTTCTGCGC AGCTTGCTAA AGAAATACAA TTAAAACATC GTGCTCCTGT GGTGGGTATT
    TCTATTTTTG ATCAGGCGGG TAGCCCTGTC GATCAGCTGA ACGCCGGTGA AAACGGGAGT
    CCACCGCATC GTGTACTTAT TGCTTCCGAG GAACAGTTCA AGGTGTTTTC ACTTCCGCAA
    CTAAAGCCGA TTAACAAATA TAAGCTTACC GCTAACGAAG GTGCTCGGAT TCGCCGCATC
    CATTTTGGTT CGTTTAGTTG TCGCATATCC CCGGAAACAC TGCAGAGTAT GCACGGTTGT
    AGCCCAACTA AGTCCACGCG TTCACATGGC GATGGAGAGG CGGATCCTAA TATCAGTGGA
    AGCTTGGCTG TAAGTCGTGG AGATGTATAT AACGAAACAG CATTGATATG TTTAACGAAT
    ATGGGCGATA TCATGGTTTT ATCAGTACCT GAATTAAAAA GACAGCTGAA TGCCGCAGCA
    GTGCGACGGG AAGACATTAA TGGAGTTTCG TCACTTTGCT TTACAAACTC TGGAGAAGCA
    CTGTATATGA TGTCTTCTTC TGAACTGCAG CGTATTGCTT TAGCCACGTC CAGAGTCGTG
    CAACCCACTG GCGTTGTTCC AGTAGAACCA TTAGAAAATG AAGAGTCTGT GTTGGAAGAA
    AATGATGCAG AGAATAATAA GGAAACCTAC GCATGTGATG AAGTTGTGAA TACATATGAA
    ATTAAAAATC CATCAGGCAT TTCAATATGC ACAAGGCCTG CAGAGGAAAA CGTTGGAAGA
    AATAGTGTTC AGCAAGTTAA TGGAGTCAAC ATTTCAAATT CACCTAATCA AGCTAACGAG
    ACTATCAGCA GCTCTATTGG CGATATTACC GTTGACTCGG TGCGCGACCA TTTAAATATG
    ACGACCACCA CTTTGTGTTC TATTAATACA GAGGAAACCA TTGGTCGCCT ATCTGTACTT
    AGCACGCAAA CCAACAAAGC CAGTACTACC GTAAACATGA GTGAAATTCC AAATATTAAT
    ATTTCTAATT TAGAGGACTT GGAATCGAAA AGAAATACGA CGGAAACGAG TACTAGTTCT
    GTTGTAATTA AATCTATAAT TACAAACATT TCTCATGAAA AAACGAACGG AGACAACAAA
    ATAGGAACGC CAAAAACAGC GCCTGAAGAA AGCCAATTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_722583.1
    CDS318..3779
    Translation

    Target ORF information:

    RefSeq Version NM_164348.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster lethal (2) giant larvae (l(2)gl), transcript variant B, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_164348.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTTAAAGT TTATCAGAGG AAAAGGGCAG CAGCCCAGTG CTGACAGACA ACGCTACATC 
    ACGGCACAGC ATGGCTTTCC TCATAAGCCT TCGGCTCTTG CGTATGATCC AGTTTTGAAA
    CTTATGGCAA TAGGGACGCA AACAGGGGCT TTAAAAGTTT TCGGTCAACC CGGAGTTGAA
    TTGTACGGTC AGCATACTTT GTTAAACAAT TCAGCATCGG AGCTTAATGT ACAATTACTT
    GAATGGGTGT ATGGAACTGG TCGCATACTT TCGTTGACGG CAGCGAATCA ATTAATTCTA
    TGGGAGCCAG TTGGAGCAAC GTTGCTGCCA ATCAAAACAC TACCGTTTGA CGGCAAACTT
    AAAAAAGTTT CATCGCTGTG CTGTTCTCTC AGTAAGGATC TGCTATGGAT TGGAACAGAA
    GGTGGAAACA TCTATCAACT GGATTTACAT ACATTTACCA TTAAGGAGCC TGTAATTTAC
    CATGACGTTG TGCTAGAGCA GGTGCCACCA GCCTACAAGC TAAATCCTGG TGCAATTGAG
    TCAATCCGCC AACTTCCAAA CTCCCCTAGC AAACTTCTAG TTGCATACAA TCGCGGCCTT
    TGTGTTTTGT GGGATTTTGA AAGCGCATCT GTCCAGCGAG CATACATAGC CCCTGGACAT
    GGACAGAGCG TTGGTCTTAC AGTGAACTTC GAAGGATCTG AATTTACCTG GTACCACGCT
    GATGGTTCAT ACGCCACTTG GAGCATAGAT AACCCAGAAC CGCCGTCGAA TGTTAATTAT
    GTGCCTTATG GACCTGATCC ATGCAAAAGC ATAAATCGAC TGTACAAAGG CAAGCGAAGA
    TCCAACGATG TAATTGTTTT TTCCGGCGGC ATGCCACGGT CAGCATATGG TGATCACAAT
    TGTGTGTCCG TTCACGCCAG CGATGGACAC AAAGTGTGTC TTGACTTTAC GTCTAAAGTG
    ATTGACTTTT TTGTGACCTT TGAAAATAAT AGAGATGTCG CTGAAGTTCT TGTTGTACTA
    CTTGAAGAGG AACTCTGCGC TTACGATCTT ACTGACCCTA ATATTTGTGC TATCAAAGCG
    CCATATCTTC ACTCTGTCCA TGCATCAGCT GTCACTTGCA ATTACCTTGC TTCTGAAGTC
    GTACAGTCGG TATATGAAAG TATTTTAAGA GCTGGAGATG AACAAGACAT TGACTATAGC
    AATATTAGCT GGCCTATCAC TGGCGGTACT CTCCCGGATA ACTTAGAAGA ATCTGTAGAA
    GAGGACGCGA CTAAGCTTTA TGAGATTTTG TTAACTGGTC ACGAAGATGG TTCTGTTAAA
    TTTTGGGACT GCACTGGAGT GTTGCTTAAA CCAATTTATA ATTTTAAAAC TAGCAGCATT
    TTTGGAAGTG AGTCAGACTT CCGAGATGAC GCAGCTGCAG ATATGAGTGC GGAACAAGTC
    GATGAAGGAG AACCGCCATT TCGGAAATCA GGACTTTTTG ATCCTTATTC AGATGACCCT
    CGTTTAGCAG TGAAGAAAAT AGCATTCTGC CCAAAAACCG GACAACTTAT TGTTGGTGGC
    ACAGCGGGCC AAATAGTTAT AGCCGACTTC ATAGACTTAC CCGAAAAAGT GTCTTTAAAA
    TACATTTCAA TGAATTTGGT CAGCGATCGT GATGGATTTG TGTGGAAGGG TCACGATCAG
    TTAAACGTGC GATCGAACTT ATTAGACGGA GAAGCAATTC CTACGACGGA ACGTGGTGTA
    AATATATCGG GAGTACTGCA AGTTTTGCCG CCAGCCAGCA TAACATGCAT GGCACTCGAA
    GCAAGCTGGG GCCTAGTATC TGGTGGGACT GCGCACGGCT TAGTTCTCTT TGACTTCAAA
    AACTTTGTTC CAGTATTTCA TCGCTGCACT TTAAACCCAA ATGATCTTAC TGGAGCAGGA
    GAGCAGCTGT CTCGTCGAAA GTCTTTTAAG AAATCATTGA GGGAGTCATT TAGAAAGCTT
    CGCAAGGGTC GATCGACCAG GACCAACCAG AGCAATCAAG TACCAACAAC GCTGGAAGCA
    AGACCCGTCG AGAGGCAAAT AGAGGCTCGT TGTGCAGATG ACGGGCTAGG ATCCATGGTG
    CGATGTTTAC TATTTGCCAA AACTTATGTT ACTAATGTCA ACATAACGTC GCCAACTTTG
    TGGTCAGCAA CAAATGCCAG TACAGTCTCG GTTTTCCTTC TGCATTTGCC ACCAGCGCAG
    ACCGCGGCAA CTGCCGTCCC GTCGGCAAGT GGCAATGCAC CACCACACAT GCCCCGCCGA
    ATTTCTGCGC AGCTTGCTAA AGAAATACAA TTAAAACATC GTGCTCCTGT GGTGGGTATT
    TCTATTTTTG ATCAGGCGGG TAGCCCTGTC GATCAGCTGA ACGCCGGTGA AAACGGGAGT
    CCACCGCATC GTGTACTTAT TGCTTCCGAG GAACAGTTCA AGGTGTTTTC ACTTCCGCAA
    CTAAAGCCGA TTAACAAATA TAAGCTTACC GCTAACGAAG GTGCTCGGAT TCGCCGCATC
    CATTTTGGTT CGTTTAGTTG TCGCATATCC CCGGAAACAC TGCAGAGTAT GCACGGTTGT
    AGCCCAACTA AGTCCACGCG TTCACATGGC GATGGAGAGG CGGATCCTAA TATCAGTGGA
    AGCTTGGCTG TAAGTCGTGG AGATGTATAT AACGAAACAG CATTGATATG TTTAACGAAT
    ATGGGCGATA TCATGGTTTT ATCAGTACCT GAATTAAAAA GACAGCTGAA TGCCGCAGCA
    GTGCGACGGG AAGACATTAA TGGAGTTTCG TCACTTTGCT TTACAAACTC TGGAGAAGCA
    CTGTATATGA TGTCTTCTTC TGAACTGCAG CGTATTGCTT TAGCCACGTC CAGAGTCGTG
    CAACCCACTG GCGTTGTTCC AGTAGAACCA TTAGAAAATG AAGAGTCTGT GTTGGAAGAA
    AATGATGCAG AGAATAATAA GGAAACCTAC GCATGTGATG AAGTTGTGAA TACATATGAA
    ATTAAAAATC CATCAGGCAT TTCAATATGC ACAAGGCCTG CAGAGGAAAA CGTTGGAAGA
    AATAGTGTTC AGCAAGTTAA TGGAGTCAAC ATTTCAAATT CACCTAATCA AGCTAACGAG
    ACTATCAGCA GCTCTATTGG CGATATTACC GTTGACTCGG TGCGCGACCA TTTAAATATG
    ACGACCACCA CTTTGTGTTC TATTAATACA GAGGAAACCA TTGGTCGCCT ATCTGTACTT
    AGCACGCAAA CCAACAAAGC CAGTACTACC GTAAACATGA GTGAAATTCC AAATATTAAT
    ATTTCTAATT TAGAGGACTT GGAATCGAAA AGAAATACGA CGGAAACGAG TACTAGTTCT
    GTTGTAATTA AATCTATAAT TACAAACATT TCTCATGAAA AAACGAACGG AGACAACAAA
    ATAGGAACGC CAAAAACAGC GCCTGAAGAA AGCCAATTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.