S6kII cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	S6kII
Entrez Gene ID	33139
Full Name	Ribosomal protein S6 kinase II
Synonyms	CG17596,Dmel\CG17596,RSK,Rsk,S6KII,dRSK,ign,rsk,rsk2
General protein information	Preferred Names Ribosomal protein S6 kinase II Names CG17596 gene product from transcript CG17596-RB CG17596-PA CG17596-PB RPS6-protein kinase-II Ribosomal-S6-kinase S6 kinase II S6kII-PA S6kII-PB ignorant p90 ribosomal S6 kinase ribosomal protein S6 kinase II
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 20C1-20C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001272850.1	NP_001259779.1	ribosomal protein S6 kinase II, isoform B
NM_078713.3	NP_523437.2	ribosomal protein S6 kinase II, isoform A

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Rps6ka3	NP_683747.1
Xenopus tropicalis (tropical clawed frog)	LOC100496161	XP_002936090.2
Canis lupus familiaris (dog)	RPS6KA3	XP_005641244.1
Rattus norvegicus (Norway rat)	Rps6ka3	NP_001178933.1
Homo sapiens (human)	RPS6KA3	NP_004577.1
Bos taurus (cattle)	RPS6KA3	NP_001092407.1
Danio rerio (zebrafish)	rps6ka3b	NP_001076495.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003040	XP_311835.5
Pan troglodytes (chimpanzee)	RPS6KA3	XP_003317432.2
	LOC696825	XP_002808568.1
Gallus gallus (chicken)	RPS6KA3	XP_004934729.1
Drosophila melanogaster (fruit fly)	S6kII	NP_523437.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

RSK2 is required for normal synaptic morphology and function. Loss of RSK2 function interferes with ERK signaling at different levels, interfering with synaptic function and axonal transport in Drosophila motoneurons.
Title: Loss of the Coffin-Lowry syndrome-associated gene RSK2 alters ERK activity, synaptic function and axonal transport in Drosophila motoneurons.

This study demonistrated that the visual orientation memory of Drosophila requires Foraging (PKG) upstream of Ignorant (RSK2) in ring neurons of the central complex.
Title: The visual orientation memory of Drosophila requires Foraging (PKG) upstream of Ignorant (RSK2) in ring neurons of the central complex.

the phosphorylation of S6KII residues, in vivo, does not follow a strict sequential pattern, as implied by certain cell-based studies of mammalian RSK protein.
Title: The C-terminal kinase and ERK-binding domains of Drosophila S6KII (RSK) are required for phosphorylation of the protein and modulation of circadian behavior.

In mutants with a full deletion or an N-terminal partial deletion of rsk, an increased bouton number at the neuromuscular junction is found.
Title: Drosophila RSK negatively regulates bouton number at the neuromuscular junction.

There is a novel regulatory mechanism of the Ras/ERK pathway by RSK, which negatively regulates ERK activity by acting as a cytoplasmic anchor in Drosophila
Title: Inhibition of ERK-MAP kinase signaling by RSK during Drosophila development.

The following S6kII gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the S6kII cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa02276	NM_078713.3 Latest version!	Drosophila melanogaster ribosomal protein S6 kinase II, transcript variant A (S6kII), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa02276	NM_001272850.1 Latest version!	Drosophila melanogaster ribosomal protein S6 kinase II, transcript variant B (S6kII), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

S6kII ( NM_001272850.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259779.1 Drosophila melanogaster ribosomal protein S6 kinase II, transcript variant B (S6kII), mRNA.

Price & Availability↑

CloneID	OFa02276
Clone ID Related Accession (Same CDS sequence)	NM_001272850.1 , NM_078713.3
Accession Version	NM_001272850.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2736bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ribosomal protein S6 kinase II, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGCTGG CCGATTCGCA AAAGGATCTC CGCCAGCAGC CTCAGCAGCA GCAGCAGCAG 
CAGCATGTGT CCTCCACCAG CAGCAGCAAC AATGCGGAGC AGCAATGCAG CAGCAGCGGA 
TTGGGTCTGC AGCTGCGCCA GCGCATGCAA ATTACCTCGT CCGGCTGCAG CAGCCTGGCG 
GTCACGCCCA TGGAGCACAC GCCCACCGAG GACGATGAGA GTGGCGGAGG CAACAGCGGT 
GTCACTTCCT CGGTGACCAC TGTGACATCA TCGCAACGCC GCCAGCAGCT GCAACAGGTG 
CAACAGCAAT CTGCTCTGCA AGCGGCCCTC GAGCAGCATC ACATCTCACC AACAGCAGAC 
TTGGATTCCG CTAGGAAAAG GCCGACGCAT CGAACCTTGT GCCCGCCGCC AGAACTTATG 
GAGCTGAGTG ATTCCGAGTC CCAGGGAGGC GTGGAAACTG GCGGTAGGAG AGAAGGGGCT 
ACTGGGCGAA GTGCACCTGA TTTAGAAGAC ACCGAGCCCC TATATGAAAC AGAAAACGAA 
TTCGAGCTTA AGGAAGTCAT CAAGGAGGGT CACGACAAGG CCGATCCTTC CCAGTTCGAG 
CTCCTACGGG TTCTGGGCGA AGGTAGCTTT GGAAAGGTGT TTCTAGTGCG AAAGATCATA 
GGCAAAGATG CAGGAACACT CTATGCCATG AAGGTGCTCA AAAAGGCCAC CCTAAAAGTA 
AAAGATCGCG TAAGGAGCAC AAATGAACGA AAAATACTAG CGGACGTGGG TCATGCTTTC 
ATCGTACGTC TTCACTATGC CTTCCAAACT CCCGGAAAAC TCTACTTGAT ACTGGATTTT 
CTTCGTGGCG GTGATCTGTT TACCCGTCTA TCCAAAGAAG TAATGTTTAC GGAAGAAGAT 
GTCAAGTTCT ATTTAGCGGA ACTGGCGCTA GCTATGAATC ACCTACACAC ATTGGGCATT 
ATCTACAGGG ATCTGAAACC GGAAAATATT CTACTGGACG AGCATGGCCA TATAGCCTTG 
ACGGACTTTG GTCTATCCAA GCAGCCTTTG GATGGCTCAA AAACATATAG CTTTTGTGGA 
ACCGTAGAAT ACATGGCGCC GGAGATCGTG AACCGAAAGG GACACGATTT TGCCGCTGAT 
TGGTGGAGTT TCGGGGTGCT CATGTACGAA ATGTTAACGG GGAATTTACC CTTTCATGGC 
CAAACCCGCC AAGAGACTAT GAATCAGATC CTTAGAAGTA AGCTGGGCAT GCCGGAGAAT 
TTGTCGCCAG AGGCGCAATC CCTGCTACGT GCTCTCTTCA AAAGAAACCC CCAGAATCGT 
TTGGGTGCGG GTGCCCAAGG AATTCTGGAC ATCAAGGCGC ACTGCTTTTT TGCCACCATC 
GACTGGGTGA GATTAGAACG AAAGCAGGTG CGTCCGCCTT TCATACCGGC GGTTAGCCGT 
GACGATGCCT TTTACTTTGA TGTGGAGTAC ACCTCAAAGT CCCCCAGGGA TTCTCCGGGT 
GGCCCGATCT CCGCATCTGC CCATGAGATC TTCCGCGGGT TCAGCTTTGT GGCTCCTGTC 
CTTCTGGAAG GTCAGTGTGC TGGCTCGAAT ATGTGCTCCA CCAGCGCCAG TCCTCTGCAT 
AGTATAGCTC CTATTCCTGC TGCTCCAGTG GGAGCCCCTC GAACATTACC TGGTGTTCTT 
CCCGGAAACT TCCATGCGGA ATATAATCTA CTGCAAGAAC TGGGACGTGG AACCTTTTCA 
GTTTGTCGGT TGTGCGAGCA TCGAGCCTCC AAGAAACATT ACGCAGTAAA AGTAATCGAA 
AAGGCAGCTG TGGCCGCAGC ATCCACATCC ACTTCTGCCG ATTGTTGGGA GGAGGTGGAG 
ATTATGCTGA GGTACGGCAA CCACCCAAAT ATCGTCACTC TGTACTCTGT TTACGAGGAT 
GCGGGGTCCG CATATCTTGT GATGGAGCTG CTTAAGGGTG GCGAGCTTCT CGATCGGATA 
CTTGCCGTGG GCCAGATGTG CGAGAGTGAG GCCAGCGCGG TGTTAAGGAC AATTGCATCT 
GCGGTAGCAT ATCTCCATGA ACATGGCGTG GTCCATCGAG ATCTTAAGCC TTCAAATATG 
ATATATGCCA GTATGCGGCA AACTCCCGAG ACCCTAAAGC TCTGCGATTT GGGTTTCGCG 
AAGCAGCTGC GCGCGGACAA CGGCCTCCTG ATGACGCCAT GCTACACAGC CAATTTTGTG 
GCTCCCGAGG TTCTAAAGAG ACAGGGCTAT GACCTGGCTT GCGACATCTG GTCGCTCGGT 
GTGCTGCTTT ACATCATGTT ATCCGGCCGG ACGCCTTTCG CCAGCACTCC AAATGATTCA 
CCGGACGTAA TACTGAAGCG CATCGGATCA GGACAAATTG ACTTCACAAG CAGTCGCTGG 
GCACTGATCA GTGTGCCGGC CAAAGAACTT TTGCGTCAGA TGCTACACAT AGTACCGGAG 
AATCGGCCGA CGGCGGCGCG AATACTTGAG CACGACTGGC TGCGGGAGCA ATTCGCCGGC 
GGCGTACAGC TTACAGAGTA TGCGGTGGCG CCCGGATCCC AACTTTCGCT GGGCGCCCAG 
CAGCAGCAGC AGAATCACAT CTCCATGGCC TTAAGAGGCG CTGTTGATGC CACTTTCCGG 
GCTATTGCCA TACCCCAGGC GGCGAATGTG GGACCCGTAG AACTTTCCAT GCTCGCCAAG 
AGGCGGGCCA AAGATCGAGC CAACCTGCAC TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259779.1
CDS	388..3123
Translation	MPLADSQKDLRQQPQQQQQQQHVSSTSSSNNAEQQCSSSGLGLQLRQRMQITSSGCSSLAVTPMEHTPTEDDESGGGNSGVTSSVTTVTSSQRRQQLQQVQQQSALQAALEQHHISPTADLDSARKRPTHRTLCPPPELMELSDSESQGGVETGGRREGATGRSAPDLEDTEPLYETENEFELKEVIKEGHDKADPSQFELLRVLGEGSFGKVFLVRKIIGKDAGTLYAMKVLKKATLKVKDRVRSTNERKILADVGHAFIVRLHYAFQTPGKLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALAMNHLHTLGIIYRDLKPENILLDEHGHIALTDFGLSKQPLDGSKTYSFCGTVEYMAPEIVNRKGHDFAADWWSFGVLMYEMLTGNLPFHGQTRQETMNQILRSKLGMPENLSPEAQSLLRALFKRNPQNRLGAGAQGILDIKAHCFFATIDWVRLERKQVRPPFIPAVSRDDAFYFDVEYTSKSPRDSPGGPISASAHEIFRGFSFVAPVLLEGQCAGSNMCSTSASPLHSIAPIPAAPVGAPRTLPGVLPGNFHAEYNLLQELGRGTFSVCRLCEHRASKKHYAVKVIEKAAVAAASTSTSADCWEEVEIMLRYGNHPNIVTLYSVYEDAGSAYLVMELLKGGELLDRILAVGQMCESEASAVLRTIASAVAYLHEHGVVHRDLKPSNMIYASMRQTPETLKLCDLGFAKQLRADNGLLMTPCYTANFVAPEVLKRQGYDLACDIWSLGVLLYIMLSGRTPFASTPNDSPDVILKRIGSGQIDFTSSRWALISVPAKELLRQMLHIVPENRPTAARILEHDWLREQFAGGVQLTEYAVAPGSQLSLGAQQQQQNHISMALRGAVDATFRAIAIPQAANVGPVELSMLAKRRAKDRANLHS

Target ORF information:

RefSeq Version	NM_001272850.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ribosomal protein S6 kinase II, transcript variant B (S6kII), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272850.1

ORF Insert Sequence:

ATGCCGCTGG CCGATTCGCA AAAGGATCTC CGCCAGCAGC CTCAGCAGCA GCAGCAGCAG 
CAGCATGTGT CCTCCACCAG CAGCAGCAAC AATGCGGAGC AGCAATGCAG CAGCAGCGGA 
TTGGGTCTGC AGCTGCGCCA GCGCATGCAA ATTACCTCGT CCGGCTGCAG CAGCCTGGCG 
GTCACGCCCA TGGAGCACAC GCCCACCGAG GACGATGAGA GTGGCGGAGG CAACAGCGGT 
GTCACTTCCT CGGTGACCAC TGTGACATCA TCGCAACGCC GCCAGCAGCT GCAACAGGTG 
CAACAGCAAT CTGCTCTGCA AGCGGCCCTC GAGCAGCATC ACATCTCACC AACAGCAGAC 
TTGGATTCCG CTAGGAAAAG GCCGACGCAT CGAACCTTGT GCCCGCCGCC AGAACTTATG 
GAGCTGAGTG ATTCCGAGTC CCAGGGAGGC GTGGAAACTG GCGGTAGGAG AGAAGGGGCT 
ACTGGGCGAA GTGCACCTGA TTTAGAAGAC ACCGAGCCCC TATATGAAAC AGAAAACGAA 
TTCGAGCTTA AGGAAGTCAT CAAGGAGGGT CACGACAAGG CCGATCCTTC CCAGTTCGAG 
CTCCTACGGG TTCTGGGCGA AGGTAGCTTT GGAAAGGTGT TTCTAGTGCG AAAGATCATA 
GGCAAAGATG CAGGAACACT CTATGCCATG AAGGTGCTCA AAAAGGCCAC CCTAAAAGTA 
AAAGATCGCG TAAGGAGCAC AAATGAACGA AAAATACTAG CGGACGTGGG TCATGCTTTC 
ATCGTACGTC TTCACTATGC CTTCCAAACT CCCGGAAAAC TCTACTTGAT ACTGGATTTT 
CTTCGTGGCG GTGATCTGTT TACCCGTCTA TCCAAAGAAG TAATGTTTAC GGAAGAAGAT 
GTCAAGTTCT ATTTAGCGGA ACTGGCGCTA GCTATGAATC ACCTACACAC ATTGGGCATT 
ATCTACAGGG ATCTGAAACC GGAAAATATT CTACTGGACG AGCATGGCCA TATAGCCTTG 
ACGGACTTTG GTCTATCCAA GCAGCCTTTG GATGGCTCAA AAACATATAG CTTTTGTGGA 
ACCGTAGAAT ACATGGCGCC GGAGATCGTG AACCGAAAGG GACACGATTT TGCCGCTGAT 
TGGTGGAGTT TCGGGGTGCT CATGTACGAA ATGTTAACGG GGAATTTACC CTTTCATGGC 
CAAACCCGCC AAGAGACTAT GAATCAGATC CTTAGAAGTA AGCTGGGCAT GCCGGAGAAT 
TTGTCGCCAG AGGCGCAATC CCTGCTACGT GCTCTCTTCA AAAGAAACCC CCAGAATCGT 
TTGGGTGCGG GTGCCCAAGG AATTCTGGAC ATCAAGGCGC ACTGCTTTTT TGCCACCATC 
GACTGGGTGA GATTAGAACG AAAGCAGGTG CGTCCGCCTT TCATACCGGC GGTTAGCCGT 
GACGATGCCT TTTACTTTGA TGTGGAGTAC ACCTCAAAGT CCCCCAGGGA TTCTCCGGGT 
GGCCCGATCT CCGCATCTGC CCATGAGATC TTCCGCGGGT TCAGCTTTGT GGCTCCTGTC 
CTTCTGGAAG GTCAGTGTGC TGGCTCGAAT ATGTGCTCCA CCAGCGCCAG TCCTCTGCAT 
AGTATAGCTC CTATTCCTGC TGCTCCAGTG GGAGCCCCTC GAACATTACC TGGTGTTCTT 
CCCGGAAACT TCCATGCGGA ATATAATCTA CTGCAAGAAC TGGGACGTGG AACCTTTTCA 
GTTTGTCGGT TGTGCGAGCA TCGAGCCTCC AAGAAACATT ACGCAGTAAA AGTAATCGAA 
AAGGCAGCTG TGGCCGCAGC ATCCACATCC ACTTCTGCCG ATTGTTGGGA GGAGGTGGAG 
ATTATGCTGA GGTACGGCAA CCACCCAAAT ATCGTCACTC TGTACTCTGT TTACGAGGAT 
GCGGGGTCCG CATATCTTGT GATGGAGCTG CTTAAGGGTG GCGAGCTTCT CGATCGGATA 
CTTGCCGTGG GCCAGATGTG CGAGAGTGAG GCCAGCGCGG TGTTAAGGAC AATTGCATCT 
GCGGTAGCAT ATCTCCATGA ACATGGCGTG GTCCATCGAG ATCTTAAGCC TTCAAATATG 
ATATATGCCA GTATGCGGCA AACTCCCGAG ACCCTAAAGC TCTGCGATTT GGGTTTCGCG 
AAGCAGCTGC GCGCGGACAA CGGCCTCCTG ATGACGCCAT GCTACACAGC CAATTTTGTG 
GCTCCCGAGG TTCTAAAGAG ACAGGGCTAT GACCTGGCTT GCGACATCTG GTCGCTCGGT 
GTGCTGCTTT ACATCATGTT ATCCGGCCGG ACGCCTTTCG CCAGCACTCC AAATGATTCA 
CCGGACGTAA TACTGAAGCG CATCGGATCA GGACAAATTG ACTTCACAAG CAGTCGCTGG 
GCACTGATCA GTGTGCCGGC CAAAGAACTT TTGCGTCAGA TGCTACACAT AGTACCGGAG 
AATCGGCCGA CGGCGGCGCG AATACTTGAG CACGACTGGC TGCGGGAGCA ATTCGCCGGC 
GGCGTACAGC TTACAGAGTA TGCGGTGGCG CCCGGATCCC AACTTTCGCT GGGCGCCCAG 
CAGCAGCAGC AGAATCACAT CTCCATGGCC TTAAGAGGCG CTGTTGATGC CACTTTCCGG 
GCTATTGCCA TACCCCAGGC GGCGAATGTG GGACCCGTAG AACTTTCCAT GCTCGCCAAG 
AGGCGGGCCA AAGATCGAGC CAACCTGCAC TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

S6kII ( NM_078713.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523437.2 Drosophila melanogaster ribosomal protein S6 kinase II, transcript variant A (S6kII), mRNA.

Price & Availability↑

CloneID	OFa02276
Clone ID Related Accession (Same CDS sequence)	NM_001272850.1 , NM_078713.3
Accession Version	NM_078713.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2736bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ribosomal protein S6 kinase II, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_078713.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGCTGG CCGATTCGCA AAAGGATCTC CGCCAGCAGC CTCAGCAGCA GCAGCAGCAG 
CAGCATGTGT CCTCCACCAG CAGCAGCAAC AATGCGGAGC AGCAATGCAG CAGCAGCGGA 
TTGGGTCTGC AGCTGCGCCA GCGCATGCAA ATTACCTCGT CCGGCTGCAG CAGCCTGGCG 
GTCACGCCCA TGGAGCACAC GCCCACCGAG GACGATGAGA GTGGCGGAGG CAACAGCGGT 
GTCACTTCCT CGGTGACCAC TGTGACATCA TCGCAACGCC GCCAGCAGCT GCAACAGGTG 
CAACAGCAAT CTGCTCTGCA AGCGGCCCTC GAGCAGCATC ACATCTCACC AACAGCAGAC 
TTGGATTCCG CTAGGAAAAG GCCGACGCAT CGAACCTTGT GCCCGCCGCC AGAACTTATG 
GAGCTGAGTG ATTCCGAGTC CCAGGGAGGC GTGGAAACTG GCGGTAGGAG AGAAGGGGCT 
ACTGGGCGAA GTGCACCTGA TTTAGAAGAC ACCGAGCCCC TATATGAAAC AGAAAACGAA 
TTCGAGCTTA AGGAAGTCAT CAAGGAGGGT CACGACAAGG CCGATCCTTC CCAGTTCGAG 
CTCCTACGGG TTCTGGGCGA AGGTAGCTTT GGAAAGGTGT TTCTAGTGCG AAAGATCATA 
GGCAAAGATG CAGGAACACT CTATGCCATG AAGGTGCTCA AAAAGGCCAC CCTAAAAGTA 
AAAGATCGCG TAAGGAGCAC AAATGAACGA AAAATACTAG CGGACGTGGG TCATGCTTTC 
ATCGTACGTC TTCACTATGC CTTCCAAACT CCCGGAAAAC TCTACTTGAT ACTGGATTTT 
CTTCGTGGCG GTGATCTGTT TACCCGTCTA TCCAAAGAAG TAATGTTTAC GGAAGAAGAT 
GTCAAGTTCT ATTTAGCGGA ACTGGCGCTA GCTATGAATC ACCTACACAC ATTGGGCATT 
ATCTACAGGG ATCTGAAACC GGAAAATATT CTACTGGACG AGCATGGCCA TATAGCCTTG 
ACGGACTTTG GTCTATCCAA GCAGCCTTTG GATGGCTCAA AAACATATAG CTTTTGTGGA 
ACCGTAGAAT ACATGGCGCC GGAGATCGTG AACCGAAAGG GACACGATTT TGCCGCTGAT 
TGGTGGAGTT TCGGGGTGCT CATGTACGAA ATGTTAACGG GGAATTTACC CTTTCATGGC 
CAAACCCGCC AAGAGACTAT GAATCAGATC CTTAGAAGTA AGCTGGGCAT GCCGGAGAAT 
TTGTCGCCAG AGGCGCAATC CCTGCTACGT GCTCTCTTCA AAAGAAACCC CCAGAATCGT 
TTGGGTGCGG GTGCCCAAGG AATTCTGGAC ATCAAGGCGC ACTGCTTTTT TGCCACCATC 
GACTGGGTGA GATTAGAACG AAAGCAGGTG CGTCCGCCTT TCATACCGGC GGTTAGCCGT 
GACGATGCCT TTTACTTTGA TGTGGAGTAC ACCTCAAAGT CCCCCAGGGA TTCTCCGGGT 
GGCCCGATCT CCGCATCTGC CCATGAGATC TTCCGCGGGT TCAGCTTTGT GGCTCCTGTC 
CTTCTGGAAG GTCAGTGTGC TGGCTCGAAT ATGTGCTCCA CCAGCGCCAG TCCTCTGCAT 
AGTATAGCTC CTATTCCTGC TGCTCCAGTG GGAGCCCCTC GAACATTACC TGGTGTTCTT 
CCCGGAAACT TCCATGCGGA ATATAATCTA CTGCAAGAAC TGGGACGTGG AACCTTTTCA 
GTTTGTCGGT TGTGCGAGCA TCGAGCCTCC AAGAAACATT ACGCAGTAAA AGTAATCGAA 
AAGGCAGCTG TGGCCGCAGC ATCCACATCC ACTTCTGCCG ATTGTTGGGA GGAGGTGGAG 
ATTATGCTGA GGTACGGCAA CCACCCAAAT ATCGTCACTC TGTACTCTGT TTACGAGGAT 
GCGGGGTCCG CATATCTTGT GATGGAGCTG CTTAAGGGTG GCGAGCTTCT CGATCGGATA 
CTTGCCGTGG GCCAGATGTG CGAGAGTGAG GCCAGCGCGG TGTTAAGGAC AATTGCATCT 
GCGGTAGCAT ATCTCCATGA ACATGGCGTG GTCCATCGAG ATCTTAAGCC TTCAAATATG 
ATATATGCCA GTATGCGGCA AACTCCCGAG ACCCTAAAGC TCTGCGATTT GGGTTTCGCG 
AAGCAGCTGC GCGCGGACAA CGGCCTCCTG ATGACGCCAT GCTACACAGC CAATTTTGTG 
GCTCCCGAGG TTCTAAAGAG ACAGGGCTAT GACCTGGCTT GCGACATCTG GTCGCTCGGT 
GTGCTGCTTT ACATCATGTT ATCCGGCCGG ACGCCTTTCG CCAGCACTCC AAATGATTCA 
CCGGACGTAA TACTGAAGCG CATCGGATCA GGACAAATTG ACTTCACAAG CAGTCGCTGG 
GCACTGATCA GTGTGCCGGC CAAAGAACTT TTGCGTCAGA TGCTACACAT AGTACCGGAG 
AATCGGCCGA CGGCGGCGCG AATACTTGAG CACGACTGGC TGCGGGAGCA ATTCGCCGGC 
GGCGTACAGC TTACAGAGTA TGCGGTGGCG CCCGGATCCC AACTTTCGCT GGGCGCCCAG 
CAGCAGCAGC AGAATCACAT CTCCATGGCC TTAAGAGGCG CTGTTGATGC CACTTTCCGG 
GCTATTGCCA TACCCCAGGC GGCGAATGTG GGACCCGTAG AACTTTCCAT GCTCGCCAAG 
AGGCGGGCCA AAGATCGAGC CAACCTGCAC TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523437.2
CDS	388..3123
Translation	MPLADSQKDLRQQPQQQQQQQHVSSTSSSNNAEQQCSSSGLGLQLRQRMQITSSGCSSLAVTPMEHTPTEDDESGGGNSGVTSSVTTVTSSQRRQQLQQVQQQSALQAALEQHHISPTADLDSARKRPTHRTLCPPPELMELSDSESQGGVETGGRREGATGRSAPDLEDTEPLYETENEFELKEVIKEGHDKADPSQFELLRVLGEGSFGKVFLVRKIIGKDAGTLYAMKVLKKATLKVKDRVRSTNERKILADVGHAFIVRLHYAFQTPGKLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALAMNHLHTLGIIYRDLKPENILLDEHGHIALTDFGLSKQPLDGSKTYSFCGTVEYMAPEIVNRKGHDFAADWWSFGVLMYEMLTGNLPFHGQTRQETMNQILRSKLGMPENLSPEAQSLLRALFKRNPQNRLGAGAQGILDIKAHCFFATIDWVRLERKQVRPPFIPAVSRDDAFYFDVEYTSKSPRDSPGGPISASAHEIFRGFSFVAPVLLEGQCAGSNMCSTSASPLHSIAPIPAAPVGAPRTLPGVLPGNFHAEYNLLQELGRGTFSVCRLCEHRASKKHYAVKVIEKAAVAAASTSTSADCWEEVEIMLRYGNHPNIVTLYSVYEDAGSAYLVMELLKGGELLDRILAVGQMCESEASAVLRTIASAVAYLHEHGVVHRDLKPSNMIYASMRQTPETLKLCDLGFAKQLRADNGLLMTPCYTANFVAPEVLKRQGYDLACDIWSLGVLLYIMLSGRTPFASTPNDSPDVILKRIGSGQIDFTSSRWALISVPAKELLRQMLHIVPENRPTAARILEHDWLREQFAGGVQLTEYAVAPGSQLSLGAQQQQQNHISMALRGAVDATFRAIAIPQAANVGPVELSMLAKRRAKDRANLHS

Target ORF information:

RefSeq Version	NM_078713.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ribosomal protein S6 kinase II, transcript variant A (S6kII), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078713.3

ORF Insert Sequence:

ATGCCGCTGG CCGATTCGCA AAAGGATCTC CGCCAGCAGC CTCAGCAGCA GCAGCAGCAG 
CAGCATGTGT CCTCCACCAG CAGCAGCAAC AATGCGGAGC AGCAATGCAG CAGCAGCGGA 
TTGGGTCTGC AGCTGCGCCA GCGCATGCAA ATTACCTCGT CCGGCTGCAG CAGCCTGGCG 
GTCACGCCCA TGGAGCACAC GCCCACCGAG GACGATGAGA GTGGCGGAGG CAACAGCGGT 
GTCACTTCCT CGGTGACCAC TGTGACATCA TCGCAACGCC GCCAGCAGCT GCAACAGGTG 
CAACAGCAAT CTGCTCTGCA AGCGGCCCTC GAGCAGCATC ACATCTCACC AACAGCAGAC 
TTGGATTCCG CTAGGAAAAG GCCGACGCAT CGAACCTTGT GCCCGCCGCC AGAACTTATG 
GAGCTGAGTG ATTCCGAGTC CCAGGGAGGC GTGGAAACTG GCGGTAGGAG AGAAGGGGCT 
ACTGGGCGAA GTGCACCTGA TTTAGAAGAC ACCGAGCCCC TATATGAAAC AGAAAACGAA 
TTCGAGCTTA AGGAAGTCAT CAAGGAGGGT CACGACAAGG CCGATCCTTC CCAGTTCGAG 
CTCCTACGGG TTCTGGGCGA AGGTAGCTTT GGAAAGGTGT TTCTAGTGCG AAAGATCATA 
GGCAAAGATG CAGGAACACT CTATGCCATG AAGGTGCTCA AAAAGGCCAC CCTAAAAGTA 
AAAGATCGCG TAAGGAGCAC AAATGAACGA AAAATACTAG CGGACGTGGG TCATGCTTTC 
ATCGTACGTC TTCACTATGC CTTCCAAACT CCCGGAAAAC TCTACTTGAT ACTGGATTTT 
CTTCGTGGCG GTGATCTGTT TACCCGTCTA TCCAAAGAAG TAATGTTTAC GGAAGAAGAT 
GTCAAGTTCT ATTTAGCGGA ACTGGCGCTA GCTATGAATC ACCTACACAC ATTGGGCATT 
ATCTACAGGG ATCTGAAACC GGAAAATATT CTACTGGACG AGCATGGCCA TATAGCCTTG 
ACGGACTTTG GTCTATCCAA GCAGCCTTTG GATGGCTCAA AAACATATAG CTTTTGTGGA 
ACCGTAGAAT ACATGGCGCC GGAGATCGTG AACCGAAAGG GACACGATTT TGCCGCTGAT 
TGGTGGAGTT TCGGGGTGCT CATGTACGAA ATGTTAACGG GGAATTTACC CTTTCATGGC 
CAAACCCGCC AAGAGACTAT GAATCAGATC CTTAGAAGTA AGCTGGGCAT GCCGGAGAAT 
TTGTCGCCAG AGGCGCAATC CCTGCTACGT GCTCTCTTCA AAAGAAACCC CCAGAATCGT 
TTGGGTGCGG GTGCCCAAGG AATTCTGGAC ATCAAGGCGC ACTGCTTTTT TGCCACCATC 
GACTGGGTGA GATTAGAACG AAAGCAGGTG CGTCCGCCTT TCATACCGGC GGTTAGCCGT 
GACGATGCCT TTTACTTTGA TGTGGAGTAC ACCTCAAAGT CCCCCAGGGA TTCTCCGGGT 
GGCCCGATCT CCGCATCTGC CCATGAGATC TTCCGCGGGT TCAGCTTTGT GGCTCCTGTC 
CTTCTGGAAG GTCAGTGTGC TGGCTCGAAT ATGTGCTCCA CCAGCGCCAG TCCTCTGCAT 
AGTATAGCTC CTATTCCTGC TGCTCCAGTG GGAGCCCCTC GAACATTACC TGGTGTTCTT 
CCCGGAAACT TCCATGCGGA ATATAATCTA CTGCAAGAAC TGGGACGTGG AACCTTTTCA 
GTTTGTCGGT TGTGCGAGCA TCGAGCCTCC AAGAAACATT ACGCAGTAAA AGTAATCGAA 
AAGGCAGCTG TGGCCGCAGC ATCCACATCC ACTTCTGCCG ATTGTTGGGA GGAGGTGGAG 
ATTATGCTGA GGTACGGCAA CCACCCAAAT ATCGTCACTC TGTACTCTGT TTACGAGGAT 
GCGGGGTCCG CATATCTTGT GATGGAGCTG CTTAAGGGTG GCGAGCTTCT CGATCGGATA 
CTTGCCGTGG GCCAGATGTG CGAGAGTGAG GCCAGCGCGG TGTTAAGGAC AATTGCATCT 
GCGGTAGCAT ATCTCCATGA ACATGGCGTG GTCCATCGAG ATCTTAAGCC TTCAAATATG 
ATATATGCCA GTATGCGGCA AACTCCCGAG ACCCTAAAGC TCTGCGATTT GGGTTTCGCG 
AAGCAGCTGC GCGCGGACAA CGGCCTCCTG ATGACGCCAT GCTACACAGC CAATTTTGTG 
GCTCCCGAGG TTCTAAAGAG ACAGGGCTAT GACCTGGCTT GCGACATCTG GTCGCTCGGT 
GTGCTGCTTT ACATCATGTT ATCCGGCCGG ACGCCTTTCG CCAGCACTCC AAATGATTCA 
CCGGACGTAA TACTGAAGCG CATCGGATCA GGACAAATTG ACTTCACAAG CAGTCGCTGG 
GCACTGATCA GTGTGCCGGC CAAAGAACTT TTGCGTCAGA TGCTACACAT AGTACCGGAG 
AATCGGCCGA CGGCGGCGCG AATACTTGAG CACGACTGGC TGCGGGAGCA ATTCGCCGGC 
GGCGTACAGC TTACAGAGTA TGCGGTGGCG CCCGGATCCC AACTTTCGCT GGGCGCCCAG 
CAGCAGCAGC AGAATCACAT CTCCATGGCC TTAAGAGGCG CTGTTGATGC CACTTTCCGG 
GCTATTGCCA TACCCCAGGC GGCGAATGTG GGACCCGTAG AACTTTCCAT GCTCGCCAAG 
AGGCGGGCCA AAGATCGAGC CAACCTGCAC TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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S6kII cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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