run cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	run
Entrez Gene ID	33059
Full Name	runt
Synonyms	1,2,AA33,CG1849,Dmel\CG1849,LB5,P235,RUN,Rnt,Run,Runt,l(1)19Ea,l(1)AA33,l(1)B2/13.1,l(1)LB9,lLB5,leg
General protein information	Preferred Names runt Names CG1849 gene product from transcript CG1849-RC CG1849-PA CG1849-PB CG1849-PC legless run-PA run-PB run-PC section 1 section 2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 19E2-19E2\|1-65 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_078700.4	NP_523424.2	runt, isoform A
NM_001298572.1	NP_001285501.1	runt, isoform C
NM_001258857.2	NP_001245786.1	runt, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Runt-dependent regulation in the Drosophila blastoderm embryo relies on unique, target-gene-specific molecular interactions.
Title: Hairless is a cofactor for Runt-dependent transcriptional regulation.

Lilli plays an architectural role in facilitating transcriptional activation that depends both on the target gene and the developmental context
Title: The HMG-box protein Lilliputian is required for Runt-dependent activation of the pair-rule gene fushi-tarazu.

Contrasting responses of different downstream targets to Runt in the presence or absence of Ftz is thus central to the combinatorial logic of the pair-rule to segment-polarity transition.
Title: Ftz modulates Runt-dependent activation and repression of segment-polarity gene transcription.

These proteins provide a paradigm for understanding DNA-binding-independent regulation by developmentally important transcription factors.
Title: A DNA-binding-independent pathway of repression by the Drosophila Runt protein.

there are two distinct steps in the repression of engrailed by Runt
Title: Distinct in vivo requirements for establishment versus maintenance of transcriptional repression.

The following run gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the run cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa02214	NM_001258857.2 Latest version!	Drosophila melanogaster runt (run), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OFa03225	NM_001298572.1 Latest version!	Drosophila melanogaster runt (run), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OFa03225	NM_078700.4 Latest version!	Drosophila melanogaster runt (run), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

run ( NM_001258857.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245786.1 Drosophila melanogaster runt (run), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa02214
Clone ID Related Accession (Same CDS sequence)	NM_001258857.2
Accession Version	NM_001258857.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	runt, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001258857.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATCTGC CAGCGGGTCC AACGATGGTG GCCAACAACA CACAGGTCCT GGCCGCTGCC 
GCCGCCGCAG CAGCCGCCGC AGCGGCCGCG GTAGCCCAGG GTCCTGGGCC GCAGCAGAGT 
AGCAATGCCA CCACCGCCTC CGCAATCGCC ATCAATCCCG CCCAGAGTCT GGCCAACACG 
AGCACCCACT CGGCCAGCAG CACGGGCAGC TCCACACCAG ATCTCAGCAC GAACAACACC 
AGCTCGAGCA GCAACGCCAC CACCAGTCCG CAGAACTCCG CCAAGATGCC CAGCTCGATG 
ACCGACATGT TCGCCAGCCT GCACGAGATG CTGCAGGAGT ATCACGGCGA GCTGGCCCAG 
ACCGGATCGC CCTCGATCCT CTGCAGCGCC CTGCCCAATC ACTGGCGGTC GAACAAGTCG 
CTGCCCGGAG CCTTCAAGGT GATCGCCCTG GACGACGTGC CCGATGGCAC CCTGGTGTCC 
ATCAAGTGCG GCAACGACGA AAACTACTGC GGCGAGCTGA GGAACTGCAC CACGACCATG 
AAGAACCAGG TGGCCAAGTT CAACGATCTG CGCTTCGTGG GACGATCGGG ACGCGGCAAG 
TCCTTCACGC TGACCATCAC CATTGCCACC TATCCGGTGC AGATCGCCAG CTACAGCAAA 
GCCATCAAGG TGACCGTCGA CGGGCCACGG GAGCCAAGAA GCTATGGCTA TCCCCATCCC 
GGCGCGTTTA ACCCGTTCAT GCTGAATCCC GCTTGGCTGG ATGCGGCGTA CATGACCTAC 
GGCTATGCGG ACTACTTCCG CCACCAGGCA GCCGCCCAGG CGGCCCAGGT GCATCATCCG 
GCACTGGCCA AGTCCTCGGC TTCCTCGGTG TCGCCAAATC CCAATCCATC GGTGGCCACC 
AGCTCCTCGT CGGCGGTTCA GCCATCTGAG TATCCCCATC CGGCGGCAGC AGTGGCAGCG 
GCGGCGGGAC AACCGGCGGC CATGATGCCA TCGCCACCGG GAGCGGCGCC TGCCACGCCC 
TATGCCATTC CGCAGTTCCC ATTCAACCAT GTGGCCGCCG CAGCCGCCGC CAAGGCAGCC 
ACGCCACACG CCTTCCATCC GTATAACTTT GCCGCGGCAG CTGGCTTGCG GGCCCGCAAT 
GCTGCCCTGC ACCACCAGAG CGAGCCGGTC CACGTCAGCC CCGCCTCCTC CAGGCCCTCC 
AGCTCCTCGC CCACCCAGCA ACACGTCCTG CTGAAGCTAA ACACCTCCAT CGAGACGAGC 
TCCATCCACG AGCAGTCCGC CAGTGACGGC GACTCCGACG ACGAGCAGAT CGACGTGGTC 
AAATCGGAGT TCGACCTGGA CAAGAGCCTG GATGTGGCGC CACTTCGCAT GCGCTGCGAC 
CTGAAGGCGC CCTCGGCCAT GAAGCCGCTC TACCACGAGA GCGGTCCTGG AGCGGTAGCC 
AACAGCCGCC AGCCGTCGCC GGAAACGACC ACCAAGATCA AGAGCGCCGC CGTGCAGCAG 
AAGACCGTGT GGCGGCCCTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245786.1
CDS	255..1778
Translation	MHLPAGPTMVANNTQVLAAAAAAAAAAAAAVAQGPGPQQSSNATTASAIAINPAQSLANTSTHSASSTGSSTPDLSTNNTSSSSNATTSPQNSAKMPSSMTDMFASLHEMLQEYHGELAQTGSPSILCSALPNHWRSNKSLPGAFKVIALDDVPDGTLVSIKCGNDENYCGELRNCTTTMKNQVAKFNDLRFVGRSGRGKSFTLTITIATYPVQIASYSKAIKVTVDGPREPRSYGYPHPGAFNPFMLNPAWLDAAYMTYGYADYFRHQAAAQAAQVHHPALAKSSASSVSPNPNPSVATSSSSAVQPSEYPHPAAAVAAAAGQPAAMMPSPPGAAPATPYAIPQFPFNHVAAAAAAKAATPHAFHPYNFAAAAGLRARNAALHHQSEPVHVSPASSRPSSSSPTQQHVLLKLNTSIETSSIHEQSASDGDSDDEQIDVVKSEFDLDKSLDVAPLRMRCDLKAPSAMKPLYHESGPGAVANSRQPSPETTTKIKSAAVQQKTVWRPY

Target ORF information:

RefSeq Version	NM_001258857.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster runt (run), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258857.2

ORF Insert Sequence:

ATGCATCTGC CAGCGGGTCC AACGATGGTG GCCAACAACA CACAGGTCCT GGCCGCTGCC 
GCCGCCGCAG CAGCCGCCGC AGCGGCCGCG GTAGCCCAGG GTCCTGGGCC GCAGCAGAGT 
AGCAATGCCA CCACCGCCTC CGCAATCGCC ATCAATCCCG CCCAGAGTCT GGCCAACACG 
AGCACCCACT CGGCCAGCAG CACGGGCAGC TCCACACCAG ATCTCAGCAC GAACAACACC 
AGCTCGAGCA GCAACGCCAC CACCAGTCCG CAGAACTCCG CCAAGATGCC CAGCTCGATG 
ACCGACATGT TCGCCAGCCT GCACGAGATG CTGCAGGAGT ATCACGGCGA GCTGGCCCAG 
ACCGGATCGC CCTCGATCCT CTGCAGCGCC CTGCCCAATC ACTGGCGGTC GAACAAGTCG 
CTGCCCGGAG CCTTCAAGGT GATCGCCCTG GACGACGTGC CCGATGGCAC CCTGGTGTCC 
ATCAAGTGCG GCAACGACGA AAACTACTGC GGCGAGCTGA GGAACTGCAC CACGACCATG 
AAGAACCAGG TGGCCAAGTT CAACGATCTG CGCTTCGTGG GACGATCGGG ACGCGGCAAG 
TCCTTCACGC TGACCATCAC CATTGCCACC TATCCGGTGC AGATCGCCAG CTACAGCAAA 
GCCATCAAGG TGACCGTCGA CGGGCCACGG GAGCCAAGAA GCTATGGCTA TCCCCATCCC 
GGCGCGTTTA ACCCGTTCAT GCTGAATCCC GCTTGGCTGG ATGCGGCGTA CATGACCTAC 
GGCTATGCGG ACTACTTCCG CCACCAGGCA GCCGCCCAGG CGGCCCAGGT GCATCATCCG 
GCACTGGCCA AGTCCTCGGC TTCCTCGGTG TCGCCAAATC CCAATCCATC GGTGGCCACC 
AGCTCCTCGT CGGCGGTTCA GCCATCTGAG TATCCCCATC CGGCGGCAGC AGTGGCAGCG 
GCGGCGGGAC AACCGGCGGC CATGATGCCA TCGCCACCGG GAGCGGCGCC TGCCACGCCC 
TATGCCATTC CGCAGTTCCC ATTCAACCAT GTGGCCGCCG CAGCCGCCGC CAAGGCAGCC 
ACGCCACACG CCTTCCATCC GTATAACTTT GCCGCGGCAG CTGGCTTGCG GGCCCGCAAT 
GCTGCCCTGC ACCACCAGAG CGAGCCGGTC CACGTCAGCC CCGCCTCCTC CAGGCCCTCC 
AGCTCCTCGC CCACCCAGCA ACACGTCCTG CTGAAGCTAA ACACCTCCAT CGAGACGAGC 
TCCATCCACG AGCAGTCCGC CAGTGACGGC GACTCCGACG ACGAGCAGAT CGACGTGGTC 
AAATCGGAGT TCGACCTGGA CAAGAGCCTG GATGTGGCGC CACTTCGCAT GCGCTGCGAC 
CTGAAGGCGC CCTCGGCCAT GAAGCCGCTC TACCACGAGA GCGGTCCTGG AGCGGTAGCC 
AACAGCCGCC AGCCGTCGCC GGAAACGACC ACCAAGATCA AGAGCGCCGC CGTGCAGCAG 
AAGACCGTGT GGCGGCCCTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

run ( NM_078700.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523424.2 Drosophila melanogaster runt (run), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa03225
Clone ID Related Accession (Same CDS sequence)	NM_078700.4 , NM_001298572.1
Accession Version	NM_078700.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1533bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	runt, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_078700.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATCTGC CAGCGGGTCC AACGATGGTG GCCAACAACA CACAGGTCCT GGCCGCTGCC 
GCCGCCGCAG CAGCCGCCGC AGCGGCCGCG GTAGCCCAGG GTCCTGGGCC GCAGCAGAGT 
AGCAATGCCA CCACCGCCTC CGCAATCGCC ATCAATCCCG CCCAGAGTCT GGCCAACACG 
AGCACCCACT CGGCCAGCAG CACGGGCAGC TCCACACCAG ATCTCAGCAC GAACAACACC 
AGCTCGAGCA GCAACGCCAC CACCAGTCCG CAGAACTCCG CCAAGATGCC CAGCTCGATG 
ACCGACATGT TCGCCAGCCT GCACGAGATG CTGCAGGAGT ATCACGGCGA GCTGGCCCAG 
ACCGGATCGC CCTCGATCCT CTGCAGCGCC CTGCCCAATC ACTGGCGGTC GAACAAGTCG 
CTGCCCGGAG CCTTCAAGGT GATCGCCCTG GACGACGTGC CCGATGGCAC CCTGGTGTCC 
ATCAAGTGCG GCAACGACGA AAACTACTGC GGCGAGCTGA GGAACTGCAC CACGACCATG 
AAGAACCAGG TGGCCAAGTT CAACGATCTG CGCTTCGTGG GACGATCGGG ACGCGGCAAG 
TCCTTCACGC TGACCATCAC CATTGCCACC TATCCGGTGC AGATCGCCAG CTACAGCAAA 
GCCATCAAGG TGACCGTCGA CGGGCCACGG GAGCCAAGAA GTAAGCAAAG CTATGGCTAT 
CCCCATCCCG GCGCGTTTAA CCCGTTCATG CTGAATCCCG CTTGGCTGGA TGCGGCGTAC 
ATGACCTACG GCTATGCGGA CTACTTCCGC CACCAGGCAG CCGCCCAGGC GGCCCAGGTG 
CATCATCCGG CACTGGCCAA GTCCTCGGCT TCCTCGGTGT CGCCAAATCC CAATCCATCG 
GTGGCCACCA GCTCCTCGTC GGCGGTTCAG CCATCTGAGT ATCCCCATCC GGCGGCAGCA 
GTGGCAGCGG CGGCGGGACA ACCGGCGGCC ATGATGCCAT CGCCACCGGG AGCGGCGCCT 
GCCACGCCCT ATGCCATTCC GCAGTTCCCA TTCAACCATG TGGCCGCCGC AGCCGCCGCC 
AAGGCAGCCA CGCCACACGC CTTCCATCCG TATAACTTTG CCGCGGCAGC TGGCTTGCGG 
GCCCGCAATG CTGCCCTGCA CCACCAGAGC GAGCCGGTCC ACGTCAGCCC CGCCTCCTCC 
AGGCCCTCCA GCTCCTCGCC CACCCAGCAA CACGTCCTGC TGAAGCTAAA CACCTCCATC 
GAGACGAGCT CCATCCACGA GCAGTCCGCC AGTGACGGCG ACTCCGACGA CGAGCAGATC 
GACGTGGTCA AATCGGAGTT CGACCTGGAC AAGAGCCTGG ATGTGGCGCC ACTTCGCATG 
CGCTGCGACC TGAAGGCGCC CTCGGCCATG AAGCCGCTCT ACCACGAGAG CGGTCCTGGA 
GCGGTAGCCA ACAGCCGCCA GCCGTCGCCG GAAACGACCA CCAAGATCAA GAGCGCCGCC 
GTGCAGCAGA AGACCGTGTG GCGGCCCTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523424.2
CDS	255..1787
Translation	MHLPAGPTMVANNTQVLAAAAAAAAAAAAAVAQGPGPQQSSNATTASAIAINPAQSLANTSTHSASSTGSSTPDLSTNNTSSSSNATTSPQNSAKMPSSMTDMFASLHEMLQEYHGELAQTGSPSILCSALPNHWRSNKSLPGAFKVIALDDVPDGTLVSIKCGNDENYCGELRNCTTTMKNQVAKFNDLRFVGRSGRGKSFTLTITIATYPVQIASYSKAIKVTVDGPREPRSKQSYGYPHPGAFNPFMLNPAWLDAAYMTYGYADYFRHQAAAQAAQVHHPALAKSSASSVSPNPNPSVATSSSSAVQPSEYPHPAAAVAAAAGQPAAMMPSPPGAAPATPYAIPQFPFNHVAAAAAAKAATPHAFHPYNFAAAAGLRARNAALHHQSEPVHVSPASSRPSSSSPTQQHVLLKLNTSIETSSIHEQSASDGDSDDEQIDVVKSEFDLDKSLDVAPLRMRCDLKAPSAMKPLYHESGPGAVANSRQPSPETTTKIKSAAVQQKTVWRPY

Target ORF information:

RefSeq Version	NM_078700.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster runt (run), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078700.4

ORF Insert Sequence:

ATGCATCTGC CAGCGGGTCC AACGATGGTG GCCAACAACA CACAGGTCCT GGCCGCTGCC 
GCCGCCGCAG CAGCCGCCGC AGCGGCCGCG GTAGCCCAGG GTCCTGGGCC GCAGCAGAGT 
AGCAATGCCA CCACCGCCTC CGCAATCGCC ATCAATCCCG CCCAGAGTCT GGCCAACACG 
AGCACCCACT CGGCCAGCAG CACGGGCAGC TCCACACCAG ATCTCAGCAC GAACAACACC 
AGCTCGAGCA GCAACGCCAC CACCAGTCCG CAGAACTCCG CCAAGATGCC CAGCTCGATG 
ACCGACATGT TCGCCAGCCT GCACGAGATG CTGCAGGAGT ATCACGGCGA GCTGGCCCAG 
ACCGGATCGC CCTCGATCCT CTGCAGCGCC CTGCCCAATC ACTGGCGGTC GAACAAGTCG 
CTGCCCGGAG CCTTCAAGGT GATCGCCCTG GACGACGTGC CCGATGGCAC CCTGGTGTCC 
ATCAAGTGCG GCAACGACGA AAACTACTGC GGCGAGCTGA GGAACTGCAC CACGACCATG 
AAGAACCAGG TGGCCAAGTT CAACGATCTG CGCTTCGTGG GACGATCGGG ACGCGGCAAG 
TCCTTCACGC TGACCATCAC CATTGCCACC TATCCGGTGC AGATCGCCAG CTACAGCAAA 
GCCATCAAGG TGACCGTCGA CGGGCCACGG GAGCCAAGAA GTAAGCAAAG CTATGGCTAT 
CCCCATCCCG GCGCGTTTAA CCCGTTCATG CTGAATCCCG CTTGGCTGGA TGCGGCGTAC 
ATGACCTACG GCTATGCGGA CTACTTCCGC CACCAGGCAG CCGCCCAGGC GGCCCAGGTG 
CATCATCCGG CACTGGCCAA GTCCTCGGCT TCCTCGGTGT CGCCAAATCC CAATCCATCG 
GTGGCCACCA GCTCCTCGTC GGCGGTTCAG CCATCTGAGT ATCCCCATCC GGCGGCAGCA 
GTGGCAGCGG CGGCGGGACA ACCGGCGGCC ATGATGCCAT CGCCACCGGG AGCGGCGCCT 
GCCACGCCCT ATGCCATTCC GCAGTTCCCA TTCAACCATG TGGCCGCCGC AGCCGCCGCC 
AAGGCAGCCA CGCCACACGC CTTCCATCCG TATAACTTTG CCGCGGCAGC TGGCTTGCGG 
GCCCGCAATG CTGCCCTGCA CCACCAGAGC GAGCCGGTCC ACGTCAGCCC CGCCTCCTCC 
AGGCCCTCCA GCTCCTCGCC CACCCAGCAA CACGTCCTGC TGAAGCTAAA CACCTCCATC 
GAGACGAGCT CCATCCACGA GCAGTCCGCC AGTGACGGCG ACTCCGACGA CGAGCAGATC 
GACGTGGTCA AATCGGAGTT CGACCTGGAC AAGAGCCTGG ATGTGGCGCC ACTTCGCATG 
CGCTGCGACC TGAAGGCGCC CTCGGCCATG AAGCCGCTCT ACCACGAGAG CGGTCCTGGA 
GCGGTAGCCA ACAGCCGCCA GCCGTCGCCG GAAACGACCA CCAAGATCAA GAGCGCCGCC 
GTGCAGCAGA AGACCGTGTG GCGGCCCTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

run ( NM_001298572.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285501.1 Drosophila melanogaster runt (run), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa03225
Clone ID Related Accession (Same CDS sequence)	NM_078700.4 , NM_001298572.1
Accession Version	NM_001298572.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1533bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	runt, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATCTGC CAGCGGGTCC AACGATGGTG GCCAACAACA CACAGGTCCT GGCCGCTGCC 
GCCGCCGCAG CAGCCGCCGC AGCGGCCGCG GTAGCCCAGG GTCCTGGGCC GCAGCAGAGT 
AGCAATGCCA CCACCGCCTC CGCAATCGCC ATCAATCCCG CCCAGAGTCT GGCCAACACG 
AGCACCCACT CGGCCAGCAG CACGGGCAGC TCCACACCAG ATCTCAGCAC GAACAACACC 
AGCTCGAGCA GCAACGCCAC CACCAGTCCG CAGAACTCCG CCAAGATGCC CAGCTCGATG 
ACCGACATGT TCGCCAGCCT GCACGAGATG CTGCAGGAGT ATCACGGCGA GCTGGCCCAG 
ACCGGATCGC CCTCGATCCT CTGCAGCGCC CTGCCCAATC ACTGGCGGTC GAACAAGTCG 
CTGCCCGGAG CCTTCAAGGT GATCGCCCTG GACGACGTGC CCGATGGCAC CCTGGTGTCC 
ATCAAGTGCG GCAACGACGA AAACTACTGC GGCGAGCTGA GGAACTGCAC CACGACCATG 
AAGAACCAGG TGGCCAAGTT CAACGATCTG CGCTTCGTGG GACGATCGGG ACGCGGCAAG 
TCCTTCACGC TGACCATCAC CATTGCCACC TATCCGGTGC AGATCGCCAG CTACAGCAAA 
GCCATCAAGG TGACCGTCGA CGGGCCACGG GAGCCAAGAA GTAAGCAAAG CTATGGCTAT 
CCCCATCCCG GCGCGTTTAA CCCGTTCATG CTGAATCCCG CTTGGCTGGA TGCGGCGTAC 
ATGACCTACG GCTATGCGGA CTACTTCCGC CACCAGGCAG CCGCCCAGGC GGCCCAGGTG 
CATCATCCGG CACTGGCCAA GTCCTCGGCT TCCTCGGTGT CGCCAAATCC CAATCCATCG 
GTGGCCACCA GCTCCTCGTC GGCGGTTCAG CCATCTGAGT ATCCCCATCC GGCGGCAGCA 
GTGGCAGCGG CGGCGGGACA ACCGGCGGCC ATGATGCCAT CGCCACCGGG AGCGGCGCCT 
GCCACGCCCT ATGCCATTCC GCAGTTCCCA TTCAACCATG TGGCCGCCGC AGCCGCCGCC 
AAGGCAGCCA CGCCACACGC CTTCCATCCG TATAACTTTG CCGCGGCAGC TGGCTTGCGG 
GCCCGCAATG CTGCCCTGCA CCACCAGAGC GAGCCGGTCC ACGTCAGCCC CGCCTCCTCC 
AGGCCCTCCA GCTCCTCGCC CACCCAGCAA CACGTCCTGC TGAAGCTAAA CACCTCCATC 
GAGACGAGCT CCATCCACGA GCAGTCCGCC AGTGACGGCG ACTCCGACGA CGAGCAGATC 
GACGTGGTCA AATCGGAGTT CGACCTGGAC AAGAGCCTGG ATGTGGCGCC ACTTCGCATG 
CGCTGCGACC TGAAGGCGCC CTCGGCCATG AAGCCGCTCT ACCACGAGAG CGGTCCTGGA 
GCGGTAGCCA ACAGCCGCCA GCCGTCGCCG GAAACGACCA CCAAGATCAA GAGCGCCGCC 
GTGCAGCAGA AGACCGTGTG GCGGCCCTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285501.1
CDS	926..2458
Translation	MHLPAGPTMVANNTQVLAAAAAAAAAAAAAVAQGPGPQQSSNATTASAIAINPAQSLANTSTHSASSTGSSTPDLSTNNTSSSSNATTSPQNSAKMPSSMTDMFASLHEMLQEYHGELAQTGSPSILCSALPNHWRSNKSLPGAFKVIALDDVPDGTLVSIKCGNDENYCGELRNCTTTMKNQVAKFNDLRFVGRSGRGKSFTLTITIATYPVQIASYSKAIKVTVDGPREPRSKQSYGYPHPGAFNPFMLNPAWLDAAYMTYGYADYFRHQAAAQAAQVHHPALAKSSASSVSPNPNPSVATSSSSAVQPSEYPHPAAAVAAAAGQPAAMMPSPPGAAPATPYAIPQFPFNHVAAAAAAKAATPHAFHPYNFAAAAGLRARNAALHHQSEPVHVSPASSRPSSSSPTQQHVLLKLNTSIETSSIHEQSASDGDSDDEQIDVVKSEFDLDKSLDVAPLRMRCDLKAPSAMKPLYHESGPGAVANSRQPSPETTTKIKSAAVQQKTVWRPY

Target ORF information:

RefSeq Version	NM_001298572.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster runt (run), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298572.1

ORF Insert Sequence:

ATGCATCTGC CAGCGGGTCC AACGATGGTG GCCAACAACA CACAGGTCCT GGCCGCTGCC 
GCCGCCGCAG CAGCCGCCGC AGCGGCCGCG GTAGCCCAGG GTCCTGGGCC GCAGCAGAGT 
AGCAATGCCA CCACCGCCTC CGCAATCGCC ATCAATCCCG CCCAGAGTCT GGCCAACACG 
AGCACCCACT CGGCCAGCAG CACGGGCAGC TCCACACCAG ATCTCAGCAC GAACAACACC 
AGCTCGAGCA GCAACGCCAC CACCAGTCCG CAGAACTCCG CCAAGATGCC CAGCTCGATG 
ACCGACATGT TCGCCAGCCT GCACGAGATG CTGCAGGAGT ATCACGGCGA GCTGGCCCAG 
ACCGGATCGC CCTCGATCCT CTGCAGCGCC CTGCCCAATC ACTGGCGGTC GAACAAGTCG 
CTGCCCGGAG CCTTCAAGGT GATCGCCCTG GACGACGTGC CCGATGGCAC CCTGGTGTCC 
ATCAAGTGCG GCAACGACGA AAACTACTGC GGCGAGCTGA GGAACTGCAC CACGACCATG 
AAGAACCAGG TGGCCAAGTT CAACGATCTG CGCTTCGTGG GACGATCGGG ACGCGGCAAG 
TCCTTCACGC TGACCATCAC CATTGCCACC TATCCGGTGC AGATCGCCAG CTACAGCAAA 
GCCATCAAGG TGACCGTCGA CGGGCCACGG GAGCCAAGAA GTAAGCAAAG CTATGGCTAT 
CCCCATCCCG GCGCGTTTAA CCCGTTCATG CTGAATCCCG CTTGGCTGGA TGCGGCGTAC 
ATGACCTACG GCTATGCGGA CTACTTCCGC CACCAGGCAG CCGCCCAGGC GGCCCAGGTG 
CATCATCCGG CACTGGCCAA GTCCTCGGCT TCCTCGGTGT CGCCAAATCC CAATCCATCG 
GTGGCCACCA GCTCCTCGTC GGCGGTTCAG CCATCTGAGT ATCCCCATCC GGCGGCAGCA 
GTGGCAGCGG CGGCGGGACA ACCGGCGGCC ATGATGCCAT CGCCACCGGG AGCGGCGCCT 
GCCACGCCCT ATGCCATTCC GCAGTTCCCA TTCAACCATG TGGCCGCCGC AGCCGCCGCC 
AAGGCAGCCA CGCCACACGC CTTCCATCCG TATAACTTTG CCGCGGCAGC TGGCTTGCGG 
GCCCGCAATG CTGCCCTGCA CCACCAGAGC GAGCCGGTCC ACGTCAGCCC CGCCTCCTCC 
AGGCCCTCCA GCTCCTCGCC CACCCAGCAA CACGTCCTGC TGAAGCTAAA CACCTCCATC 
GAGACGAGCT CCATCCACGA GCAGTCCGCC AGTGACGGCG ACTCCGACGA CGAGCAGATC 
GACGTGGTCA AATCGGAGTT CGACCTGGAC AAGAGCCTGG ATGTGGCGCC ACTTCGCATG 
CGCTGCGACC TGAAGGCGCC CTCGGCCATG AAGCCGCTCT ACCACGAGAG CGGTCCTGGA 
GCGGTAGCCA ACAGCCGCCA GCCGTCGCCG GAAACGACCA CCAAGATCAA GAGCGCCGCC 
GTGCAGCAGA AGACCGTGTG GCGGCCCTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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run cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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