RhoGAP19D cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	RhoGAP19D
Entrez Gene ID	33048
Full Name	Rho GTPase activating protein at 19D
Synonyms	1412,CG1412,Dmel\CG1412
General protein information	Preferred Names Rho GTPase activating protein at 19D Names CG1412 gene product from transcript CG1412-RB CG1412-PA CG1412-PB CG1412-PC CG1412-PD CG1412-PE RhoGAP19D-PA RhoGAP19D-PB RhoGAP19D-PC RhoGAP19D-PD RhoGAP19D-PE
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 19D1-19D2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001258851.1	NP_001245780.1	Rho GTPase activating protein at 19D, isoform C
NM_001169338.2	NP_001162809.1	Rho GTPase activating protein at 19D, isoform B
NM_134552.6	NP_608396.2	Rho GTPase activating protein at 19D, isoform A
NM_001298567.1	NP_001285496.1	Rho GTPase activating protein at 19D, isoform D
NM_001298568.1	NP_001285497.1	Rho GTPase activating protein at 19D, isoform E

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following RhoGAP19D gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RhoGAP19D cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa02208	NM_001169338.2 Latest version!	Drosophila melanogaster Rho GTPase activating protein at 19D (RhoGAP19D), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OFa02209	NM_001258851.1 Latest version!	Drosophila melanogaster Rho GTPase activating protein at 19D (RhoGAP19D), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OFa02209	NM_001298568.1 Latest version!	Drosophila melanogaster Rho GTPase activating protein at 19D (RhoGAP19D), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OFa03218	NM_134552.6 Latest version!	Drosophila melanogaster Rho GTPase activating protein at 19D (RhoGAP19D), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OFa21558	NM_001298567.1 Latest version!	Drosophila melanogaster Rho GTPase activating protein at 19D (RhoGAP19D), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RhoGAP19D ( NM_001169338.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001162809.1 Drosophila melanogaster Rho GTPase activating protein at 19D (RhoGAP19D), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa02208
Clone ID Related Accession (Same CDS sequence)	NM_001169338.2
Accession Version	NM_001169338.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6330bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho GTPase activating protein at 19D, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On May 8, 2012 this sequence version replaced NM_001169338.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTACAAA ACTCGAACGG CGCTGCCGCA GCGCATTCGG CAGCCTCAGC GGTGGCGACT 
CCGGCAGCGG CGGCATCCGC AGCAGCAGCA GCTGCTGCAG CGGCGGTGGC TGCAGCGGCC 
AACAACGCCG CCCTGGCCGC CTCCTCCATC CAGCCCAAGC TCATCGTCAT CCGCCGGCGA 
CCCAACCAGG GATTCGGGTT CACGCTGCGC CACTTCATCG CCTATCCGCC GGAAGATGAC 
CAGGCATCAT CATCCGCATC GGGTTTGGTA TCGGGATCGG CGACAGCAGC AACGGCGGCA 
TCGGTATCGA CCAACTGGCC ACAGGAAGCC TCCAGTGCGG CGGGCAGCAA CAGCGGCAGC 
AGCAGCAGCG TCGGCGTCGC TGGGATCACC GGTTTGGAGC CAACGTCGCC GACGTCGCTG 
CCGCCGTACC AAGTGAAAGC GATGGAGACC ATTTTCATCA AAGAGGTGCA GGCGAACGGA 
CCGGCCCACT ACGCCAACCT GCAGACGGGC GACCGCGTGC TGATGGTCAA CAACCAGCCG 
ATCGCTGGCA TCGCCTACAG CACCATCGTC TCGATGATCA AGCAGACGCC GGCGGTCCTC 
ACGCTGCACG TGGTGCCCAA GGAGTGCGAT GTCCTGCAGA TGCACTATAC GAGCATTGCC 
CACACGCCGG AGTCGAATCG CCTGACCATG TCGACGCACT CGCCCTCGCT GCTGGCCAAC 
GGCTCCCACA AGACCACATT TCCAGCGGCG GCAGCAGTTG CAGCACCACC GCATCAGCAT 
CAGCAGCAGC AGCAACAGTT GATCTCATAT CCCGCTGTTG CAGCCGTGAC CACATCAACG 
CCGGCAGGAT CGCCGCAATC CCAATCCCAA TCCCAATCGC ACTCGCACCC CAACACCCAT 
CATGCACCAT CCCTGCACGG AGGCAGTCGA TCGGGCAGCA TCACCAGCAC GGCCAGCGGA 
GGCATCACCG TCCTCAGTCA GCCGTTCTAC CCACAGCAGC AGCAGCAACA GCAACAGCAG 
CAGCAACATC GTCATCCAGC GCTGACTGGC GGCAGCAGCA GCATCGATTT CGGGGACATG 
GCACATGGAC TGCGCCAGCA TCAGCAGCAG CAGCAACATC TGTACCAGCA GCAGCAACAG 
CATCACCAGT TTATGCGTGC TAATCAGCCG CCAAATTTAA CAGTATTATC GGCCAGCTCG 
GCAACGAACA CGCCCACACC CACCGCCCGC CAGCCCAAGT CCGCGACGAG CCACAATCAG 
GCGTTGTATG CAGTCGGAGC GGGTCCGAGC TCAGCAGCTG GATCTGGGTC GGGATCCCTT 
TCGGGGGGCA GTGAGAACAG CGACCTTATG ATCCGACTGC GCGAGTCCAT CAAGCAGAAG 
GAGGAGTTCC TTAAGTCGCC GGTGCCCGCC TCCATGAGCT CCGCCTCCTC TATCAACGGC 
AACCAGGCGC AGTCGTCATC GCCGGCACAG CAGCAACACC TGCAGCAGCA CCAGCAGCAC 
TTCTTTCCCT CGCACACGCC GCCAGGTGGT GTCCAAGTGG TGGTGCCTCC ACCGCGCAGT 
CGCCATCAAG TGATGCTCAA GCAGCAGCAG CAGCAACAGC AGCAGCACCA GCAACATCAT 
GTCCTTGGCT ACGACATGTA CCACTCCGGT GGTAAGCTGG TGCAGCGGTC TTCCACGGCG 
CCTGGCAGTG TGTGCCACCA ACACCAGCAG CAGCAGCAGC ATCACCTCTA TCACCAGCAG 
CAACATCAGC AGCTCCAGCA GACGCAACAT CGCCAGGTGA TGATCAACAA CAATGCCAAG 
TACGCCAAGG ATCTGGACTA TTTTGAGACG TTGCAGGAGA CAGGAGTCAC CAATAAGGTC 
TTTGAGCGCA AGCTGGTGTC ACACATGTCC AAGGGATTCG ATCCGTTTAA GCCGCTGTCG 
ATCAACACCA GTGCCCTGGA GTCGTCTGCC AGCACGTCGA GCACTTCGGC CACCACGACG 
GTGAAGAAGC AAAGTGTGCT GTATGAATCC CTGCAGCAGC ATCCATTGGC CAAGTACCAT 
CAGACGCAGC AGCAAACGAC AACGACGACG ACGGTGGATG GACAGACGAG TAGCCGGATG 
GAGCTGCACA AGGCGACCCT GGCGAATGCC ACCATCGATC CGGTGCCAGT TGGATCCAGC 
ACAGCCATTG TGGACAGTGC GTCGGGAAGC GGCAACGTTG TGCGGCGCTA CAAGCCATTA 
TCCTCCAGCT CCTTTGACGA AGGCAAACCA ATGCGTAGGA TATCCTACCT GCGGGCAACC 
AACAATGAGA CCGACTTCAA TGCGGAGGCT GCCGCTGGCG GAGATGCCGC GGCAACAGGG 
GCGTCAGGAC CAGCATCTGC ATCAGCAGCT TCATCTGCAC CTGGACCCAC GCCCTCATCT 
GCTCCTGCAC CAGCTCCAGC ACCAGCACCC CTGCCCATTT CGATCCCCAT TCCGGTGGCT 
GCTGTCGTCG AGCCGCAAAA GACGAAGTCC CTGGTGGAGG AGCATCCGGA TCCCACCTAC 
GATGTGATCG GAGGCACAGG TGGTGGCCAC GAGTTCATCG AGACGCCGAA CGGCCTGGAG 
GATGTGCGTC TGTCGGCCCA CGGGAGTCGG AGGGCCTCCA TGAGCAGTGA CATGCGAAGC 
AGCATCCACA TTGATGCCGA GTTAAATGGC AAGTATGTGC CAAACGAACT GGAGGCCTTT 
GAGACGGAAA TCGAGATCAA GTCCTCTTGC ATCAACGGAA AACGCATGTC CGAGTACCGA 
TCATGGCGTC AGGTGAAGCT GGAGATCAAG GGAGATCTGC TGCGTATCTA CTCAGGCCGA 
CATGCCAAAT CGGAGCACAA TGTGATAGAA CTTGATATTA GAAACTTTAA GTTCTTCGAC 
GAGTCGATGG ATAAGAAGAA ATACTTGATC CGTATGCAGT CGAAGCCATC GCCCAGTGGA 
GCCCATTTGG CCTCCTTGGG CAACGAGTTA AACCTGGACG AGGGTCACGA CAAGCTGCTA 
ACCTCGTCGG GCAGCAGCAG TGCGAATGAG CCCGCTGCCA ACGCAGCATC GGCCCTGACC 
GCATCCACCT CCTCGTCCAC CTGCAGCAGC GGTCCTTACA CGGAGATCCT ATTCAAGACG 
AAGAGCAGCA ACGAGATGAA GCGTCTGTTT GGACTTCTGC AGTGGAAGGA TAGCCTCAAC 
TACGATGATA ATGAGCACCA GCCACAGGGC AAGCAGTTGG CAGAACCACA GAAAACAGTG 
GCCAGCGCCA GTTATCTGGA CGACGTCCAA GGTGGAGCGA GTGCAGCCGA CAGCTCACCC 
TTGAGCTCCC ATTCCGGTGG CATTGCAGAC GCTAGTGGCA GTCATCATGT TTCTGCGCTA 
CCAGCAGCTG CGGCTATTGT CGCCGCCACC AGCGACTCCA TTTCGCCGGT GATGAAGGCA 
AGGAAGTCCT CTTCGCACAA GCATATACCT GATAAGGATT TGGGATCGCC CAAATCGAAG 
AACTGGAAGG ATTTGCTCTT CCGCCGTGGC GGCAGTGGCA GCGGAGGAGT GTCACACCAC 
GACTTGGCTT CGCCATCCGC CTGTGCAGCC AAGCAGATTG GATCTATTGG CGTTCCACTG 
CGCAGCTGTC CCATGTCGAA GGTCAATGCA TATGTGCCGC ATCTGGTAGA GGTGTGCACG 
AACATTGTGG AGACAAAGGG TCTGGGAGTG GTGGGCATCT ATCGCATACC CGGCAACAAG 
GCGGCCATAT CAGAGCTGTC CGAACTTGTT AACACCAAGG ACTTTCAGTT CGAGAGCTGT 
GCCAGCGATG ACCGCTGGGA GGATGTGAAT GTGGTGAGCA GCCTGCTCAA GTTGTTTATA 
CGCAGCTTGC CCGATGCCTT AATGCCGGCA AGTTACTATA TCAACTTTAT TGAGGCGGAC 
AAGAAGTTTG GCCTGGAGAG AATTGTACTG TTGCGCGAGA TTGTGGAATC GTTACCACGG 
CATCCCTACG AAACGATGAA GCACTTGATT CGGCATCTGT GCCGAGTTAG CGGCAATTGC 
GATGTGAACC GCATGGAGCC CAAGAACCTG GCCATCATAT TTGGGCCATC TATAATCAGG 
ACGCCGAACG ATACTCTGGA AACGGCAGTC AAGGACATGA AGCATCAATG CCGCATCGTT 
GAGCTGCTGG TCACACAGTA CGACTACTTC TTCGAAGGCG GCAGTCTGCC GGATATAGCC 
GAAGTAAGCG GCGGTACAGC TGCCGTAAGC GCCGCTCAGC CGCAGCAGCA GACGCAGGAG 
GATCAGACGA GCATGCTGCT GCACAACCTG TCGAAGATCG AGCGGATCAC AGAGCGCGAA 
ACCACGCGCA CCTCGCGCTT TATGCCGCAG CTCAGACGAA AGACCCATGG CAAGCGCAGC 
GCCGTAAACT CGGACACGTA CTCCGGCGAG AGCGTTCTGT CGTTGGACTA CGCCAAGGCG 
TCCGCCTCTG CCTCGGCATC CGCAAGCAGC ACCGCCAGTA CTTCGAGCAG CTCGACGCTT 
CACAGCGGGG GCAAGGCATC CGCCTCATCC TCATCCAGTT CCACTACCAA GTTCAGCTCA 
TCGTCATCGT CGGCGTGCTC AACAACGGCG GTGGCTATTT CTTCCTCCAT CTCGGCGACG 
TCCTCCAAAT TGTTAAGCGC CAATTTTAAG AAGCGTAGCA CAGGAGGTTT CTTGGGCTCA 
AGATCCTCGC ACCAGACGCG CAAGGCCAGC TTGGACGAAA AGGACTCAGG ACAGTCGTCT 
GGCAGCCTTG GTCACAGCCT GGGAGTAGGC ATAAATCTGG CCAAGGAACA ACAGCGCAGC 
AGCGTGGACA TATCCATTCT GCTAACGGAC GACGACTCTA GCAGCAGGCT AAGCGATACG 
GGTTCCATGT CGCTGACGAC CATTACGGAT ACACTGGACA GCAAGCTACG CAACTTGCGC 
AGCGGCTCCG AATCGAATGA TGAGAATGGA TCGCCCGAGT TTCCCAAGAA CCGGCGGCAC 
ACCTTGGGCC AGCCTCTGCA CATGCACTCC GAGAACATTC CGTATGCGGA CGAGTCGCCC 
GAGCGGCTGT TCCATCAGTT TTGCAATCCC CTGGAGACAG CCCCCTTATC CCTGCATCTA 
AGCCGCTCCT GCACGGCCAC AAATACAATT GGTGGCGATG AAAAGCCTTC GAAACAGTCG 
GGTGGAGTTG CACCACTGCC CCCAAGTTTG CTTAAGGCCG CACATAAGCT ACAGCACTCA 
GCCACGGTGC CCATTCATCA GGGAAGCTCC ACTGCACCAA CTAGCGGAGC AGCCACGCCA 
GTAGGCACGC CAACCGGAAG CGGTGGGGCC GGAGCTATTC CCTCGCGCAG CTCATCGAGC 
AGTGTCACGA CGCTAACCAA AAGCATACAA CCCAGGTTCA GTAACTATCT AAGCTACGAA 
AGAGGCAATG CAGCCGCTGG AGGCGCCGAA AGGGTGGTGG GATCCGCCGG AGGAGCTGCC 
TCCGACGACG ATTCCGAGGG CTCGACCACC AGTGATCCCA AGGAGAAGCT GCTTCTCGGT 
GAACGACCGT CGCCTTCGTT TTTTATCGAG CGCTACAACA AAAAGAGACG CGATCATCGG 
TTATTTCGCA GCGCCAGTTT CAATTGCCGG AACTACTCCA CCAGGCACAT AACGGCGCAG 
ACATCCGGAG GAGTAGCGGC GGGAGTATCG GGCGGAGTAG CTGGAGCGGT GGCGGTGGGG 
GTGGCTTGCT GCCAGGCATT GAGCACCACT GGCTTGACCA AGGACGAGAA GACTGATATG 
AACCTGACCA AGAAGCGCCA GATCCAGAAC AAGCAGAATC GGTCCATTAA GCGGCGGCAC 
ACAGTAGGCG GACCTCACGA CTATTCCGCC AGCAATGGTG GCTGCTCGAA TCACTCACAC 
GGTCACGACC TGGCAGCCAT CAACTACAAT CACCACAATC GCCATCACCA GCAACAGTTG 
TTGAAACTGG GCAGCGCTAC ATCTGGCGGT GGAGGCAGTG GCGCAGCCGC GGCCGAGACG 
ACGACCACCA CAACGACCAC CACCACGGTG CTGCGGCGCC TCGGTACAGG TCAAGCTGGC 
GCCGAAGCTA GTGTGGCATT GCCCGCATCA AGTTCCGGAT CGAGCAGCAA CAACAATAAC 
TCGCCCCAAA GCGATGGCAG TAATGGCAAC GGCGGTGGAG TGGCGGGAGT TGCCTCCAAT 
GCACCACCAA CTGGCGTAGG TGTCGGTGGC AACAACAGCA GCGGAAGCAG CAGCAACAGC 
AGCAGCACCA GCACAACTCC CGCTGGGAAC GGCGATCAGG TCCTGGAAGT GGGCATACGT 
ATCAAGAACA TTAGCCGGGG TCGCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162809.1
CDS	803..7132
Translation	MLQNSNGAAAAHSAASAVATPAAAASAAAAAAAAAVAAAANNAALAASSIQPKLIVIRRRPNQGFGFTLRHFIAYPPEDDQASSSASGLVSGSATAATAASVSTNWPQEASSAAGSNSGSSSSVGVAGITGLEPTSPTSLPPYQVKAMETIFIKEVQANGPAHYANLQTGDRVLMVNNQPIAGIAYSTIVSMIKQTPAVLTLHVVPKECDVLQMHYTSIAHTPESNRLTMSTHSPSLLANGSHKTTFPAAAAVAAPPHQHQQQQQQLISYPAVAAVTTSTPAGSPQSQSQSQSHSHPNTHHAPSLHGGSRSGSITSTASGGITVLSQPFYPQQQQQQQQQQQHRHPALTGGSSSIDFGDMAHGLRQHQQQQQHLYQQQQQHHQFMRANQPPNLTVLSASSATNTPTPTARQPKSATSHNQALYAVGAGPSSAAGSGSGSLSGGSENSDLMIRLRESIKQKEEFLKSPVPASMSSASSINGNQAQSSSPAQQQHLQQHQQHFFPSHTPPGGVQVVVPPPRSRHQVMLKQQQQQQQQHQQHHVLGYDMYHSGGKLVQRSSTAPGSVCHQHQQQQQHHLYHQQQHQQLQQTQHRQVMINNNAKYAKDLDYFETLQETGVTNKVFERKLVSHMSKGFDPFKPLSINTSALESSASTSSTSATTTVKKQSVLYESLQQHPLAKYHQTQQQTTTTTTVDGQTSSRMELHKATLANATIDPVPVGSSTAIVDSASGSGNVVRRYKPLSSSSFDEGKPMRRISYLRATNNETDFNAEAAAGGDAAATGASGPASASAASSAPGPTPSSAPAPAPAPAPLPISIPIPVAAVVEPQKTKSLVEEHPDPTYDVIGGTGGGHEFIETPNGLEDVRLSAHGSRRASMSSDMRSSIHIDAELNGKYVPNELEAFETEIEIKSSCINGKRMSEYRSWRQVKLEIKGDLLRIYSGRHAKSEHNVIELDIRNFKFFDESMDKKKYLIRMQSKPSPSGAHLASLGNELNLDEGHDKLLTSSGSSSANEPAANAASALTASTSSSTCSSGPYTEILFKTKSSNEMKRLFGLLQWKDSLNYDDNEHQPQGKQLAEPQKTVASASYLDDVQGGASAADSSPLSSHSGGIADASGSHHVSALPAAAAIVAATSDSISPVMKARKSSSHKHIPDKDLGSPKSKNWKDLLFRRGGSGSGGVSHHDLASPSACAAKQIGSIGVPLRSCPMSKVNAYVPHLVEVCTNIVETKGLGVVGIYRIPGNKAAISELSELVNTKDFQFESCASDDRWEDVNVVSSLLKLFIRSLPDALMPASYYINFIEADKKFGLERIVLLREIVESLPRHPYETMKHLIRHLCRVSGNCDVNRMEPKNLAIIFGPSIIRTPNDTLETAVKDMKHQCRIVELLVTQYDYFFEGGSLPDIAEVSGGTAAVSAAQPQQQTQEDQTSMLLHNLSKIERITERETTRTSRFMPQLRRKTHGKRSAVNSDTYSGESVLSLDYAKASASASASASSTASTSSSSTLHSGGKASASSSSSSTTKFSSSSSSACSTTAVAISSSISATSSKLLSANFKKRSTGGFLGSRSSHQTRKASLDEKDSGQSSGSLGHSLGVGINLAKEQQRSSVDISILLTDDDSSSRLSDTGSMSLTTITDTLDSKLRNLRSGSESNDENGSPEFPKNRRHTLGQPLHMHSENIPYADESPERLFHQFCNPLETAPLSLHLSRSCTATNTIGGDEKPSKQSGGVAPLPPSLLKAAHKLQHSATVPIHQGSSTAPTSGAATPVGTPTGSGGAGAIPSRSSSSSVTTLTKSIQPRFSNYLSYERGNAAAGGAERVVGSAGGAASDDDSEGSTTSDPKEKLLLGERPSPSFFIERYNKKRRDHRLFRSASFNCRNYSTRHITAQTSGGVAAGVSGGVAGAVAVGVACCQALSTTGLTKDEKTDMNLTKKRQIQNKQNRSIKRRHTVGGPHDYSASNGGCSNHSHGHDLAAINYNHHNRHHQQQLLKLGSATSGGGGSGAAAAETTTTTTTTTTVLRRLGTGQAGAEASVALPASSSGSSSNNNNSPQSDGSNGNGGGVAGVASNAPPTGVGVGGNNSSGSSSNSSSTSTTPAGNGDQVLEVGIRIKNISRGRF

Target ORF information:

RefSeq Version	NM_001169338.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho GTPase activating protein at 19D (RhoGAP19D), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169338.2

ORF Insert Sequence:

ATGTTACAAA ACTCGAACGG CGCTGCCGCA GCGCATTCGG CAGCCTCAGC GGTGGCGACT 
CCGGCAGCGG CGGCATCCGC AGCAGCAGCA GCTGCTGCAG CGGCGGTGGC TGCAGCGGCC 
AACAACGCCG CCCTGGCCGC CTCCTCCATC CAGCCCAAGC TCATCGTCAT CCGCCGGCGA 
CCCAACCAGG GATTCGGGTT CACGCTGCGC CACTTCATCG CCTATCCGCC GGAAGATGAC 
CAGGCATCAT CATCCGCATC GGGTTTGGTA TCGGGATCGG CGACAGCAGC AACGGCGGCA 
TCGGTATCGA CCAACTGGCC ACAGGAAGCC TCCAGTGCGG CGGGCAGCAA CAGCGGCAGC 
AGCAGCAGCG TCGGCGTCGC TGGGATCACC GGTTTGGAGC CAACGTCGCC GACGTCGCTG 
CCGCCGTACC AAGTGAAAGC GATGGAGACC ATTTTCATCA AAGAGGTGCA GGCGAACGGA 
CCGGCCCACT ACGCCAACCT GCAGACGGGC GACCGCGTGC TGATGGTCAA CAACCAGCCG 
ATCGCTGGCA TCGCCTACAG CACCATCGTC TCGATGATCA AGCAGACGCC GGCGGTCCTC 
ACGCTGCACG TGGTGCCCAA GGAGTGCGAT GTCCTGCAGA TGCACTATAC GAGCATTGCC 
CACACGCCGG AGTCGAATCG CCTGACCATG TCGACGCACT CGCCCTCGCT GCTGGCCAAC 
GGCTCCCACA AGACCACATT TCCAGCGGCG GCAGCAGTTG CAGCACCACC GCATCAGCAT 
CAGCAGCAGC AGCAACAGTT GATCTCATAT CCCGCTGTTG CAGCCGTGAC CACATCAACG 
CCGGCAGGAT CGCCGCAATC CCAATCCCAA TCCCAATCGC ACTCGCACCC CAACACCCAT 
CATGCACCAT CCCTGCACGG AGGCAGTCGA TCGGGCAGCA TCACCAGCAC GGCCAGCGGA 
GGCATCACCG TCCTCAGTCA GCCGTTCTAC CCACAGCAGC AGCAGCAACA GCAACAGCAG 
CAGCAACATC GTCATCCAGC GCTGACTGGC GGCAGCAGCA GCATCGATTT CGGGGACATG 
GCACATGGAC TGCGCCAGCA TCAGCAGCAG CAGCAACATC TGTACCAGCA GCAGCAACAG 
CATCACCAGT TTATGCGTGC TAATCAGCCG CCAAATTTAA CAGTATTATC GGCCAGCTCG 
GCAACGAACA CGCCCACACC CACCGCCCGC CAGCCCAAGT CCGCGACGAG CCACAATCAG 
GCGTTGTATG CAGTCGGAGC GGGTCCGAGC TCAGCAGCTG GATCTGGGTC GGGATCCCTT 
TCGGGGGGCA GTGAGAACAG CGACCTTATG ATCCGACTGC GCGAGTCCAT CAAGCAGAAG 
GAGGAGTTCC TTAAGTCGCC GGTGCCCGCC TCCATGAGCT CCGCCTCCTC TATCAACGGC 
AACCAGGCGC AGTCGTCATC GCCGGCACAG CAGCAACACC TGCAGCAGCA CCAGCAGCAC 
TTCTTTCCCT CGCACACGCC GCCAGGTGGT GTCCAAGTGG TGGTGCCTCC ACCGCGCAGT 
CGCCATCAAG TGATGCTCAA GCAGCAGCAG CAGCAACAGC AGCAGCACCA GCAACATCAT 
GTCCTTGGCT ACGACATGTA CCACTCCGGT GGTAAGCTGG TGCAGCGGTC TTCCACGGCG 
CCTGGCAGTG TGTGCCACCA ACACCAGCAG CAGCAGCAGC ATCACCTCTA TCACCAGCAG 
CAACATCAGC AGCTCCAGCA GACGCAACAT CGCCAGGTGA TGATCAACAA CAATGCCAAG 
TACGCCAAGG ATCTGGACTA TTTTGAGACG TTGCAGGAGA CAGGAGTCAC CAATAAGGTC 
TTTGAGCGCA AGCTGGTGTC ACACATGTCC AAGGGATTCG ATCCGTTTAA GCCGCTGTCG 
ATCAACACCA GTGCCCTGGA GTCGTCTGCC AGCACGTCGA GCACTTCGGC CACCACGACG 
GTGAAGAAGC AAAGTGTGCT GTATGAATCC CTGCAGCAGC ATCCATTGGC CAAGTACCAT 
CAGACGCAGC AGCAAACGAC AACGACGACG ACGGTGGATG GACAGACGAG TAGCCGGATG 
GAGCTGCACA AGGCGACCCT GGCGAATGCC ACCATCGATC CGGTGCCAGT TGGATCCAGC 
ACAGCCATTG TGGACAGTGC GTCGGGAAGC GGCAACGTTG TGCGGCGCTA CAAGCCATTA 
TCCTCCAGCT CCTTTGACGA AGGCAAACCA ATGCGTAGGA TATCCTACCT GCGGGCAACC 
AACAATGAGA CCGACTTCAA TGCGGAGGCT GCCGCTGGCG GAGATGCCGC GGCAACAGGG 
GCGTCAGGAC CAGCATCTGC ATCAGCAGCT TCATCTGCAC CTGGACCCAC GCCCTCATCT 
GCTCCTGCAC CAGCTCCAGC ACCAGCACCC CTGCCCATTT CGATCCCCAT TCCGGTGGCT 
GCTGTCGTCG AGCCGCAAAA GACGAAGTCC CTGGTGGAGG AGCATCCGGA TCCCACCTAC 
GATGTGATCG GAGGCACAGG TGGTGGCCAC GAGTTCATCG AGACGCCGAA CGGCCTGGAG 
GATGTGCGTC TGTCGGCCCA CGGGAGTCGG AGGGCCTCCA TGAGCAGTGA CATGCGAAGC 
AGCATCCACA TTGATGCCGA GTTAAATGGC AAGTATGTGC CAAACGAACT GGAGGCCTTT 
GAGACGGAAA TCGAGATCAA GTCCTCTTGC ATCAACGGAA AACGCATGTC CGAGTACCGA 
TCATGGCGTC AGGTGAAGCT GGAGATCAAG GGAGATCTGC TGCGTATCTA CTCAGGCCGA 
CATGCCAAAT CGGAGCACAA TGTGATAGAA CTTGATATTA GAAACTTTAA GTTCTTCGAC 
GAGTCGATGG ATAAGAAGAA ATACTTGATC CGTATGCAGT CGAAGCCATC GCCCAGTGGA 
GCCCATTTGG CCTCCTTGGG CAACGAGTTA AACCTGGACG AGGGTCACGA CAAGCTGCTA 
ACCTCGTCGG GCAGCAGCAG TGCGAATGAG CCCGCTGCCA ACGCAGCATC GGCCCTGACC 
GCATCCACCT CCTCGTCCAC CTGCAGCAGC GGTCCTTACA CGGAGATCCT ATTCAAGACG 
AAGAGCAGCA ACGAGATGAA GCGTCTGTTT GGACTTCTGC AGTGGAAGGA TAGCCTCAAC 
TACGATGATA ATGAGCACCA GCCACAGGGC AAGCAGTTGG CAGAACCACA GAAAACAGTG 
GCCAGCGCCA GTTATCTGGA CGACGTCCAA GGTGGAGCGA GTGCAGCCGA CAGCTCACCC 
TTGAGCTCCC ATTCCGGTGG CATTGCAGAC GCTAGTGGCA GTCATCATGT TTCTGCGCTA 
CCAGCAGCTG CGGCTATTGT CGCCGCCACC AGCGACTCCA TTTCGCCGGT GATGAAGGCA 
AGGAAGTCCT CTTCGCACAA GCATATACCT GATAAGGATT TGGGATCGCC CAAATCGAAG 
AACTGGAAGG ATTTGCTCTT CCGCCGTGGC GGCAGTGGCA GCGGAGGAGT GTCACACCAC 
GACTTGGCTT CGCCATCCGC CTGTGCAGCC AAGCAGATTG GATCTATTGG CGTTCCACTG 
CGCAGCTGTC CCATGTCGAA GGTCAATGCA TATGTGCCGC ATCTGGTAGA GGTGTGCACG 
AACATTGTGG AGACAAAGGG TCTGGGAGTG GTGGGCATCT ATCGCATACC CGGCAACAAG 
GCGGCCATAT CAGAGCTGTC CGAACTTGTT AACACCAAGG ACTTTCAGTT CGAGAGCTGT 
GCCAGCGATG ACCGCTGGGA GGATGTGAAT GTGGTGAGCA GCCTGCTCAA GTTGTTTATA 
CGCAGCTTGC CCGATGCCTT AATGCCGGCA AGTTACTATA TCAACTTTAT TGAGGCGGAC 
AAGAAGTTTG GCCTGGAGAG AATTGTACTG TTGCGCGAGA TTGTGGAATC GTTACCACGG 
CATCCCTACG AAACGATGAA GCACTTGATT CGGCATCTGT GCCGAGTTAG CGGCAATTGC 
GATGTGAACC GCATGGAGCC CAAGAACCTG GCCATCATAT TTGGGCCATC TATAATCAGG 
ACGCCGAACG ATACTCTGGA AACGGCAGTC AAGGACATGA AGCATCAATG CCGCATCGTT 
GAGCTGCTGG TCACACAGTA CGACTACTTC TTCGAAGGCG GCAGTCTGCC GGATATAGCC 
GAAGTAAGCG GCGGTACAGC TGCCGTAAGC GCCGCTCAGC CGCAGCAGCA GACGCAGGAG 
GATCAGACGA GCATGCTGCT GCACAACCTG TCGAAGATCG AGCGGATCAC AGAGCGCGAA 
ACCACGCGCA CCTCGCGCTT TATGCCGCAG CTCAGACGAA AGACCCATGG CAAGCGCAGC 
GCCGTAAACT CGGACACGTA CTCCGGCGAG AGCGTTCTGT CGTTGGACTA CGCCAAGGCG 
TCCGCCTCTG CCTCGGCATC CGCAAGCAGC ACCGCCAGTA CTTCGAGCAG CTCGACGCTT 
CACAGCGGGG GCAAGGCATC CGCCTCATCC TCATCCAGTT CCACTACCAA GTTCAGCTCA 
TCGTCATCGT CGGCGTGCTC AACAACGGCG GTGGCTATTT CTTCCTCCAT CTCGGCGACG 
TCCTCCAAAT TGTTAAGCGC CAATTTTAAG AAGCGTAGCA CAGGAGGTTT CTTGGGCTCA 
AGATCCTCGC ACCAGACGCG CAAGGCCAGC TTGGACGAAA AGGACTCAGG ACAGTCGTCT 
GGCAGCCTTG GTCACAGCCT GGGAGTAGGC ATAAATCTGG CCAAGGAACA ACAGCGCAGC 
AGCGTGGACA TATCCATTCT GCTAACGGAC GACGACTCTA GCAGCAGGCT AAGCGATACG 
GGTTCCATGT CGCTGACGAC CATTACGGAT ACACTGGACA GCAAGCTACG CAACTTGCGC 
AGCGGCTCCG AATCGAATGA TGAGAATGGA TCGCCCGAGT TTCCCAAGAA CCGGCGGCAC 
ACCTTGGGCC AGCCTCTGCA CATGCACTCC GAGAACATTC CGTATGCGGA CGAGTCGCCC 
GAGCGGCTGT TCCATCAGTT TTGCAATCCC CTGGAGACAG CCCCCTTATC CCTGCATCTA 
AGCCGCTCCT GCACGGCCAC AAATACAATT GGTGGCGATG AAAAGCCTTC GAAACAGTCG 
GGTGGAGTTG CACCACTGCC CCCAAGTTTG CTTAAGGCCG CACATAAGCT ACAGCACTCA 
GCCACGGTGC CCATTCATCA GGGAAGCTCC ACTGCACCAA CTAGCGGAGC AGCCACGCCA 
GTAGGCACGC CAACCGGAAG CGGTGGGGCC GGAGCTATTC CCTCGCGCAG CTCATCGAGC 
AGTGTCACGA CGCTAACCAA AAGCATACAA CCCAGGTTCA GTAACTATCT AAGCTACGAA 
AGAGGCAATG CAGCCGCTGG AGGCGCCGAA AGGGTGGTGG GATCCGCCGG AGGAGCTGCC 
TCCGACGACG ATTCCGAGGG CTCGACCACC AGTGATCCCA AGGAGAAGCT GCTTCTCGGT 
GAACGACCGT CGCCTTCGTT TTTTATCGAG CGCTACAACA AAAAGAGACG CGATCATCGG 
TTATTTCGCA GCGCCAGTTT CAATTGCCGG AACTACTCCA CCAGGCACAT AACGGCGCAG 
ACATCCGGAG GAGTAGCGGC GGGAGTATCG GGCGGAGTAG CTGGAGCGGT GGCGGTGGGG 
GTGGCTTGCT GCCAGGCATT GAGCACCACT GGCTTGACCA AGGACGAGAA GACTGATATG 
AACCTGACCA AGAAGCGCCA GATCCAGAAC AAGCAGAATC GGTCCATTAA GCGGCGGCAC 
ACAGTAGGCG GACCTCACGA CTATTCCGCC AGCAATGGTG GCTGCTCGAA TCACTCACAC 
GGTCACGACC TGGCAGCCAT CAACTACAAT CACCACAATC GCCATCACCA GCAACAGTTG 
TTGAAACTGG GCAGCGCTAC ATCTGGCGGT GGAGGCAGTG GCGCAGCCGC GGCCGAGACG 
ACGACCACCA CAACGACCAC CACCACGGTG CTGCGGCGCC TCGGTACAGG TCAAGCTGGC 
GCCGAAGCTA GTGTGGCATT GCCCGCATCA AGTTCCGGAT CGAGCAGCAA CAACAATAAC 
TCGCCCCAAA GCGATGGCAG TAATGGCAAC GGCGGTGGAG TGGCGGGAGT TGCCTCCAAT 
GCACCACCAA CTGGCGTAGG TGTCGGTGGC AACAACAGCA GCGGAAGCAG CAGCAACAGC 
AGCAGCACCA GCACAACTCC CGCTGGGAAC GGCGATCAGG TCCTGGAAGT GGGCATACGT 
ATCAAGAACA TTAGCCGGGG TCGCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RhoGAP19D ( NM_001258851.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245780.1 Drosophila melanogaster Rho GTPase activating protein at 19D (RhoGAP19D), transcript variant C, mRNA.

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CloneID	OFa02209
Clone ID Related Accession (Same CDS sequence)	NM_001258851.1 , NM_001298568.1
Accession Version	NM_001258851.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho GTPase activating protein at 19D, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTACAAA ACTCGAACGG CGCTGCCGCA GCGCATTCGG CAGCCTCAGC GGTGGCGACT 
CCGGCAGCGG CGGCATCCGC AGCAGCAGCA GCTGCTGCAG CGGCGGTGGC TGCAGCGGCC 
AACAACGCCG CCCTGGCCGC CTCCTCCATC CAGCCCAAGC TCATCGTCAT CCGCCGGCGA 
CCCAACCAGG GATTCGGGTT CACGCTGCGC CACTTCATCG CCTATCCGCC GGAAGATGAC 
CAGGCATCAT CATCCGCATC GGGTTTGGTA TCGGGATCGG CGACAGCAGC AACGGCGGCA 
TCGGTATCGA CCAACTGGCC ACAGGAAGCC TCCAGTGCGG CGGGCAGCAA CAGCGGCAGC 
AGCAGCAGCG TCGGCGTCGC TGGGATCACC GGTTTGGAGC CAACGTCGCC GACGTCGCTG 
CCGCCGTACC AAGTGAAAGC GATGGAGACC ATTTTCATCA AAGAGGTGCA GGCGAACGGA 
CCGGCCCACT ACGCCAACCT GCAGACGGGC GACCGCGTGC TGATGGTCAA CAACCAGCCG 
ATCGCTGGCA TCGCCTACAG CACCATCGTC TCGATGATCA AGCAGACGCC GGCGGTCCTC 
ACGCTGCACG TGGTGCCCAA GGAGTGCGAT GTCCTGCAGA TGCACTATAC GAGCATTGCC 
CACACGCCGG AGTCGAATCG CCTGACCATG TCGACGCACT CGCCCTCGCT GCTGGCCAAC 
GGCTCCCACA AGACCACATT TCCAGCGGCG GCAGCAGTTG CAGCACCACC GCATCAGCAT 
CAGCAGCAGC AGCAACAGTT GATCTCATAT CCCGCTGTTG CAGCCGTGAC CACATCAACG 
CCGGCAGGAT CGCCGCAATC CCAATCCCAA TCCCAATCGC ACTCGCACCC CAACACCCAT 
CATGCACCAT CCCTGCACGG AGGCAGTCGA TCGGGCAGCA TCACCAGCAC GGCCAGCGGA 
GGCATCACCG TCCTCAGTCA GCCGTTCTAC CCACAGCAGC AGCAGCAACA GCAACAGCAG 
CAGCAACATC GTCATCCAGC GCTGACTGGC GGCAGCAGCA GCATCGATTT CGGGGACATG 
GCACATGGAC TGCGCCAGCA TCAGCAGCAG CAGCAACATC TGTACCAGCA GCAGCAACAG 
CATCACCAGT TTATGCGTGC TAATCAGCCG CCAAATTTAA CAGTATTATC GGCCAGCTCG 
GCAACGAACA CGCCCACACC CACCGCCCGC CAGCCCAAGT CCGCGACGAG CCACAATCAG 
GCGTTGTATG CAGTCGGAGC GGGTCCGAGC TCAGCAGCTG GATCTGGGTC GGGATCCCTT 
TCGGGGGGCA GTGAGAACAG CGACCTTATG ATCCGACTGC GCGAGTCCAT CAAGCAGAAG 
GAGGAGTTCC TTAAGTCGCC GGTGCCCGCC TCCATGAGCT CCGCCTCCTC TATCAACGGC 
AACCAGGCGC AGTCGTCATC GCCGGCACAG CAGCAACACC TGCAGCAGCA CCAGCAGCAC 
TTCTTTCCCT CGCACACGCC GCCAGGTGGT GTCCAAGTGG TGGTGCCTCC ACCGCGCAGT 
CGCCATCAAG TGATGCTCAA GCAGCAGCAG CAGCAACAGC AGCAGCACCA GCAACATCAT 
GTCCTTGGCT ACGACATGTA CCACTCCGGT GGTAAGCTGG TGCAGCGGTC TTCCACGGCG 
CCTGGCAGTG TGTGCCACCA ACACCAGCAG CAGCAGCAGC ATCACCTCTA TCACCAGCAG 
CAACATCAGC AGCTCCAGCA GACGCAACAT CGCCAGGTGA TGATCAACAA CAATGCCAAG 
TACGCCAAGG ATCTGGACTA TTTTGAGACG TTGCAGGAGA CAGGAGTCAC CAATAAGGTC 
TTTGAGCGCA AGCTGGTGTC ACACATGTCC AAGGGATTCG ATCCGTTTAA GCCGCTGTCG 
ATCAACACCA GTGCCCTGGA GTCGTCTGCC AGCACGTCGA GCACTTCGGC CACCACGACG 
GTGAAGAAGC AAAGTGTGCT GTATGAATCC CTGCAGCAGC ATCCATTGGC CAAGTACCAT 
CAGACGCAGC AGCAAACGAC AACGACGACG ACGGTGGATG GACAGACGAG TAGCCGGATG 
GAGCTGCACA AGGCGACCCT GGCGAATGCC ACCATCGATC CGGTGCCAGT TGGATCCAGC 
AAGTTTGTGA CGCTGCCGGA TAAGCCGGAT AAGGGTTCCT CCGTTGCCAC CTTGGCGGAC 
ATCGCCGAGA GCAGTGCAGC AGCCATTGTG GACAGTGCGT CGGGAAGCGG CAACGTTGTG 
CGGCGCTACA AGCCATTATC CTCCAGCTCC TTTGACGAAG GCAAACCAAT GCGTAGGATA 
TCCTACCTGC GGGCAACCAA CAATGAGACC GACTTCAATG CGGAGGCTGC CGCTGGCGGA 
GATGCCGCGG CAACAGGGGC GTCAGGACCA GCATCTGCAT CAGCAGCTTC ATCTGCACCT 
GGACCCACGC CCTCATCTGC TCCTGCACCA GCTCCAGCAC CAGCACCCCT GCCCATTTCG 
ATCCCCATTC CGGTGGCTGC TGTCGTCGAG CCGCAAAAGA CGAAGTCCCT GGTGGAGGAG 
CATCCGGATC CCACCTACGA TGTGATCGGA GGCACAGGTG GTGGCCACGA GTTCATCGAG 
ACGCCGAACG GCCTGGAGGA TGTGCGTCTG TCGGCCCACG GGAGTCGGAG GGCCTCCATG 
AGCAGTGACA TGCGAAGCAG CATCCACATT GATGCCGAGT TAAATGGCAA GTATGTGCCA 
AACGAACTGG AGGCCTTTGA GACGGAAATC GAGATCAAGT CCTCTTGCAT CAACGGAAAA 
CGCATGTCCG AGTACCGATC ATGGCGTCAG GTGAAGCTGG AGATCAAGGG AGATCTGCTG 
CGTATCTACT CAGGCCGACA TGCCAAATCG GAGCACAATG TGATAGAACT TGATATTAGA 
AACTTTAAGT TCTTCGACGA GTCGATGGAT AAGAAGAAAT ACTTGATCCG TATGCAGTCG 
AAGCCATCGC CCAGTGGAGC CCATTTGGCC TCCTTGGGCA ACGAGTTAAA CCTGGACGAG 
GGTCACGACA AGCTGCTAAC CTCGTCGGGC AGCAGCAGTG CGAATGAGCC CGCTGCCAAC 
GCAGCATCGG CCCTGACCGC ATCCACCTCC TCGTCCACCT GCAGCAGCGG TCCTTACACG 
GAGATCCTAT TCAAGACGAA GAGCAGCAAC GAGATGAAGC GTCTGTTTGG ACTTCTGCAG 
TGGAAGGATA GCCTCAACTA CGATGATAAT GAGCACCAGC CACAGGGCAA GCAGTTGGCA 
GAACCACAGA AAACAGTGGC CAGCGCCAGT TATCTGGACG ACGTCCAAGG TGGAGCGAGT 
GCAGCCGACA GCTCACCCTT GAGCTCCCAT TCCGGTGGCA TTGCAGACGC TAGTGGCAGT 
CATCATGTTT CTGCGCTACC AGCAGCTGCG GCTATTGTCG CCGCCACCAG CGACTCCATT 
TCGCCGGTGA TGAAGGCAAG GAAGTCCTCT TCGCACAAGC ATATACCTGA TAAGGATTTG 
GGATCGCCCA AATCGAAGAA CTGGAAGGAT TTGCTCTTCC GCCGTGGCGG CAGTGGCAGC 
GGAGGAGTGT CACACCACGA CTTGGCTTCG CCATCCGCCT GTGCAGCCAA GCAGATTGGA 
TCTATTGGCG TTCCACTGCG CAGCTGTCCC ATGTCGAAGG TCAATGCATA TGTGCCGCAT 
CTGGTAGAGG TGTGCACGAA CATTGTGGAG ACAAAGGGTC TGGGAGTGGT GGGCATCTAT 
CGCATACCCG GCAACAAGGC GGCCATATCA GAGCTGTCCG AACTTGTTAA CACCAAGGAC 
TTTCAGTTCG AGAGCTGTGC CAGCGATGAC CGCTGGGAGG ATGTGAATGT GGTGAGCAGC 
CTGCTCAAGT TGTTTATACG CAGCTTGCCC GATGCCTTAA TGCCGGCAAG TTACTATATC 
AACTTTATTG AGGCGGACAA GAAGTTTGGC CTGGAGAGAA TTGTACTGTT GCGCGAGATT 
GTGGAATCGT TACCACGGCA TCCCTACGAA ACGATGAAGC ACTTGATTCG GCATCTGTGC 
CGAGTTAGCG GCAATTGCGA TGTGAACCGC ATGGAGCCCA AGAACCTGGC CATCATATTT 
GGGCCATCTA TAATCAGGAC GCCGAACGAT ACTCTGGAAA CGGCAGTCAA GGACATGAAG 
CATCAATGCC GCATCGTTGA GCTGCTGGTC ACACAGTACG ACTACTTCTT CGAAGGCGGC 
AGTCTGCCGG ATATAGCCGA AGTAAGCGGC GGTACAGCTG CCGTAAGCGC CGCTCAGCCG 
CAGCAGCAGA CGCAGGAGGA TCAGACGAGC ATGCTGCTGC ACAACCTGTC GAAGATCGAG 
CGGATCACAG AGCGCGAAAC CACGCGCACC TCGCGCTTTA TGCCGCAGCT CAGACGAAAG 
ACCCATGGCA AGCGCAGCGC CGTAAACTCG GACACGTACT CCGGCGAGAG CGTTCTGTCG 
TTGGACTACG CCAAGGCGTC CGCCTCTGCC TCGGCATCCG CAAGCAGCAC CGCCAGTACT 
TCGAGCAGCT CGACGCTTCA CAGCGGGGGC AAGGCATCCG CCTCATCCTC ATCCAGTTCC 
ACTACCAAGT TCAGCTCATC GTCATCGTCG GCGTGCTCAA CAACGGCGGT GGCTATTTCT 
TCCTCCATCT CGGCGACGTC CTCCAAATTG TTAAGCGCCA ATTTTAAGAA GCGTAGCACA 
GGAGGTTTCT TGGGCTCAAG ATCCTCGCAC CAGACGCGCA AGGCCAGCTT GGACGAAAAG 
GACTCAGGAC AGTCGTCTGG CAGCCTTGGT CACAGCCTGG GAGTAGGCAT AAATCTGGCC 
AAGGAACAAC AGCGCAGCAG CGTGGACATA TCCATTCTGC TAACGGACGA CGACTCTAGC 
AGCAGGCTAA GCGATACGGG TTCCATGTCG CTGACGACCA TTACGGATAC ACTGGACAGC 
AAGCTACGCA ACTTGCGCAG CGGCTCCGAA TCGAATGATG AGAATGGATC GCCCGAGTTT 
CCCAAGAACC GGCGGCACAC CTTGGGCCAG CCTCTGCACA TGCACTCCGA GAACATTCCG 
TATGCGGACG AGTCGCCCGA GCGGCTGTTC CATCAGTTTT GCAATCCCCT GGAGACAGCC 
CCCTTATCCC TGCATCTAAG CCGCTCCTGC ACGGCCACAA ATACAATTGG TGGCGATGAA 
AAGCCTTCGA AACAGTCGGG TGGAGTTGCA CCACTGCCCC CAAGTTTGCT TAAGGCCGCA 
CATAAGCTAC AGCACTCAGC CACGGTGCCC ATTCATCAGG GAAGCTCCAC TGCACCAACT 
AGCGGAGCAG CCACGCCAGT AGGCACGCCA ACCGGAAGCG GTGGGGCCGG AGCTATTCCC 
TCGCGCAGCT CATCGAGCAG TGTCACGACG CTAACCAAAA GCATACAACC CAGGTTCAGT 
AACTATCTAA GCTACGAAAG AGGCAATGCA GCCGCTGGAG GCGCCGAAAG GGTGGTGGGA 
TCCGCCGGAG GAGCTGCCTC CGACGACGAT TCCGAGGGCT CGACCACCAG TGATCCCAAG 
GAGAAGCTGC TTCTCGGTGA ACGACCGTCG CCTTCGTTTT TTATCGAGCG CTACAACAAA 
AAGAGACGCG ATCATCGGTT ATTTCGCAGC GCCAGTTTCA ATTGCCGGAA CTACTCCACC 
AGGCACATAA CGGCGCAGAC ATCCGGAGGA GTAGCGGCGG GAGTATCGGG CGGAGTAGCT 
GGAGCGGTGG CGGTGGGGGT GGCTTGCTGC CAGGCATTGA GCACCACTGG CTTGACCAAG 
GACGAGAAGA CTGATATGAA CCTGACCAAG AAGCGCCAGA TCCAGAACAA GCAGAATCGG 
TCCATTAAGC GGCGGCACAC AGTAGGCGGA CCTCACGACT ATTCCGCCAG CAATGGTGGC 
TGCTCGAATC ACTCACACGG TCACGACCTG GCAGCCATCA ACTACAATCA CCACAATCGC 
CATCACCAGC AACAGTTGTT GAAACTGGGC AGCGCTACAT CTGGCGGTGG AGGCAGTGGC 
GCAGCCGCGG CCGAGACGAC GACCACCACA ACGACCACCA CCACGGTGCT GCGGCGCCTC 
GGTACAGGTC AAGCTGGCGC CGAAGCTAGT GTGGCATTGC CCGCATCAAG TTCCGGATCG 
AGCAGCAACA ACAATAACTC GCCCCAAAGC GATGGCAGTA ATGGCAACGG CGGTGGAGTG 
GCGGGAGTTG CCTCCAATGC ACCACCAACT GGCGTAGGTG TCGGTGGCAA CAACAGCAGC 
GGAAGCAGCA GCAACAGCAG CAGCACCAGC ACAACTCCCG CTGGGAACGG CGATCAGGTC 
CTGGAAGTGG GCATACGTAT CAAGAACATT AGCCGGGGTC GCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245780.1
CDS	803..7210
Translation	MLQNSNGAAAAHSAASAVATPAAAASAAAAAAAAAVAAAANNAALAASSIQPKLIVIRRRPNQGFGFTLRHFIAYPPEDDQASSSASGLVSGSATAATAASVSTNWPQEASSAAGSNSGSSSSVGVAGITGLEPTSPTSLPPYQVKAMETIFIKEVQANGPAHYANLQTGDRVLMVNNQPIAGIAYSTIVSMIKQTPAVLTLHVVPKECDVLQMHYTSIAHTPESNRLTMSTHSPSLLANGSHKTTFPAAAAVAAPPHQHQQQQQQLISYPAVAAVTTSTPAGSPQSQSQSQSHSHPNTHHAPSLHGGSRSGSITSTASGGITVLSQPFYPQQQQQQQQQQQHRHPALTGGSSSIDFGDMAHGLRQHQQQQQHLYQQQQQHHQFMRANQPPNLTVLSASSATNTPTPTARQPKSATSHNQALYAVGAGPSSAAGSGSGSLSGGSENSDLMIRLRESIKQKEEFLKSPVPASMSSASSINGNQAQSSSPAQQQHLQQHQQHFFPSHTPPGGVQVVVPPPRSRHQVMLKQQQQQQQQHQQHHVLGYDMYHSGGKLVQRSSTAPGSVCHQHQQQQQHHLYHQQQHQQLQQTQHRQVMINNNAKYAKDLDYFETLQETGVTNKVFERKLVSHMSKGFDPFKPLSINTSALESSASTSSTSATTTVKKQSVLYESLQQHPLAKYHQTQQQTTTTTTVDGQTSSRMELHKATLANATIDPVPVGSSKFVTLPDKPDKGSSVATLADIAESSAAAIVDSASGSGNVVRRYKPLSSSSFDEGKPMRRISYLRATNNETDFNAEAAAGGDAAATGASGPASASAASSAPGPTPSSAPAPAPAPAPLPISIPIPVAAVVEPQKTKSLVEEHPDPTYDVIGGTGGGHEFIETPNGLEDVRLSAHGSRRASMSSDMRSSIHIDAELNGKYVPNELEAFETEIEIKSSCINGKRMSEYRSWRQVKLEIKGDLLRIYSGRHAKSEHNVIELDIRNFKFFDESMDKKKYLIRMQSKPSPSGAHLASLGNELNLDEGHDKLLTSSGSSSANEPAANAASALTASTSSSTCSSGPYTEILFKTKSSNEMKRLFGLLQWKDSLNYDDNEHQPQGKQLAEPQKTVASASYLDDVQGGASAADSSPLSSHSGGIADASGSHHVSALPAAAAIVAATSDSISPVMKARKSSSHKHIPDKDLGSPKSKNWKDLLFRRGGSGSGGVSHHDLASPSACAAKQIGSIGVPLRSCPMSKVNAYVPHLVEVCTNIVETKGLGVVGIYRIPGNKAAISELSELVNTKDFQFESCASDDRWEDVNVVSSLLKLFIRSLPDALMPASYYINFIEADKKFGLERIVLLREIVESLPRHPYETMKHLIRHLCRVSGNCDVNRMEPKNLAIIFGPSIIRTPNDTLETAVKDMKHQCRIVELLVTQYDYFFEGGSLPDIAEVSGGTAAVSAAQPQQQTQEDQTSMLLHNLSKIERITERETTRTSRFMPQLRRKTHGKRSAVNSDTYSGESVLSLDYAKASASASASASSTASTSSSSTLHSGGKASASSSSSSTTKFSSSSSSACSTTAVAISSSISATSSKLLSANFKKRSTGGFLGSRSSHQTRKASLDEKDSGQSSGSLGHSLGVGINLAKEQQRSSVDISILLTDDDSSSRLSDTGSMSLTTITDTLDSKLRNLRSGSESNDENGSPEFPKNRRHTLGQPLHMHSENIPYADESPERLFHQFCNPLETAPLSLHLSRSCTATNTIGGDEKPSKQSGGVAPLPPSLLKAAHKLQHSATVPIHQGSSTAPTSGAATPVGTPTGSGGAGAIPSRSSSSSVTTLTKSIQPRFSNYLSYERGNAAAGGAERVVGSAGGAASDDDSEGSTTSDPKEKLLLGERPSPSFFIERYNKKRRDHRLFRSASFNCRNYSTRHITAQTSGGVAAGVSGGVAGAVAVGVACCQALSTTGLTKDEKTDMNLTKKRQIQNKQNRSIKRRHTVGGPHDYSASNGGCSNHSHGHDLAAINYNHHNRHHQQQLLKLGSATSGGGGSGAAAAETTTTTTTTTTVLRRLGTGQAGAEASVALPASSSGSSSNNNNSPQSDGSNGNGGGVAGVASNAPPTGVGVGGNNSSGSSSNSSSTSTTPAGNGDQVLEVGIRIKNISRGRF

Target ORF information:

RefSeq Version	NM_001258851.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho GTPase activating protein at 19D (RhoGAP19D), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258851.1

ORF Insert Sequence:

ATGTTACAAA ACTCGAACGG CGCTGCCGCA GCGCATTCGG CAGCCTCAGC GGTGGCGACT 
CCGGCAGCGG CGGCATCCGC AGCAGCAGCA GCTGCTGCAG CGGCGGTGGC TGCAGCGGCC 
AACAACGCCG CCCTGGCCGC CTCCTCCATC CAGCCCAAGC TCATCGTCAT CCGCCGGCGA 
CCCAACCAGG GATTCGGGTT CACGCTGCGC CACTTCATCG CCTATCCGCC GGAAGATGAC 
CAGGCATCAT CATCCGCATC GGGTTTGGTA TCGGGATCGG CGACAGCAGC AACGGCGGCA 
TCGGTATCGA CCAACTGGCC ACAGGAAGCC TCCAGTGCGG CGGGCAGCAA CAGCGGCAGC 
AGCAGCAGCG TCGGCGTCGC TGGGATCACC GGTTTGGAGC CAACGTCGCC GACGTCGCTG 
CCGCCGTACC AAGTGAAAGC GATGGAGACC ATTTTCATCA AAGAGGTGCA GGCGAACGGA 
CCGGCCCACT ACGCCAACCT GCAGACGGGC GACCGCGTGC TGATGGTCAA CAACCAGCCG 
ATCGCTGGCA TCGCCTACAG CACCATCGTC TCGATGATCA AGCAGACGCC GGCGGTCCTC 
ACGCTGCACG TGGTGCCCAA GGAGTGCGAT GTCCTGCAGA TGCACTATAC GAGCATTGCC 
CACACGCCGG AGTCGAATCG CCTGACCATG TCGACGCACT CGCCCTCGCT GCTGGCCAAC 
GGCTCCCACA AGACCACATT TCCAGCGGCG GCAGCAGTTG CAGCACCACC GCATCAGCAT 
CAGCAGCAGC AGCAACAGTT GATCTCATAT CCCGCTGTTG CAGCCGTGAC CACATCAACG 
CCGGCAGGAT CGCCGCAATC CCAATCCCAA TCCCAATCGC ACTCGCACCC CAACACCCAT 
CATGCACCAT CCCTGCACGG AGGCAGTCGA TCGGGCAGCA TCACCAGCAC GGCCAGCGGA 
GGCATCACCG TCCTCAGTCA GCCGTTCTAC CCACAGCAGC AGCAGCAACA GCAACAGCAG 
CAGCAACATC GTCATCCAGC GCTGACTGGC GGCAGCAGCA GCATCGATTT CGGGGACATG 
GCACATGGAC TGCGCCAGCA TCAGCAGCAG CAGCAACATC TGTACCAGCA GCAGCAACAG 
CATCACCAGT TTATGCGTGC TAATCAGCCG CCAAATTTAA CAGTATTATC GGCCAGCTCG 
GCAACGAACA CGCCCACACC CACCGCCCGC CAGCCCAAGT CCGCGACGAG CCACAATCAG 
GCGTTGTATG CAGTCGGAGC GGGTCCGAGC TCAGCAGCTG GATCTGGGTC GGGATCCCTT 
TCGGGGGGCA GTGAGAACAG CGACCTTATG ATCCGACTGC GCGAGTCCAT CAAGCAGAAG 
GAGGAGTTCC TTAAGTCGCC GGTGCCCGCC TCCATGAGCT CCGCCTCCTC TATCAACGGC 
AACCAGGCGC AGTCGTCATC GCCGGCACAG CAGCAACACC TGCAGCAGCA CCAGCAGCAC 
TTCTTTCCCT CGCACACGCC GCCAGGTGGT GTCCAAGTGG TGGTGCCTCC ACCGCGCAGT 
CGCCATCAAG TGATGCTCAA GCAGCAGCAG CAGCAACAGC AGCAGCACCA GCAACATCAT 
GTCCTTGGCT ACGACATGTA CCACTCCGGT GGTAAGCTGG TGCAGCGGTC TTCCACGGCG 
CCTGGCAGTG TGTGCCACCA ACACCAGCAG CAGCAGCAGC ATCACCTCTA TCACCAGCAG 
CAACATCAGC AGCTCCAGCA GACGCAACAT CGCCAGGTGA TGATCAACAA CAATGCCAAG 
TACGCCAAGG ATCTGGACTA TTTTGAGACG TTGCAGGAGA CAGGAGTCAC CAATAAGGTC 
TTTGAGCGCA AGCTGGTGTC ACACATGTCC AAGGGATTCG ATCCGTTTAA GCCGCTGTCG 
ATCAACACCA GTGCCCTGGA GTCGTCTGCC AGCACGTCGA GCACTTCGGC CACCACGACG 
GTGAAGAAGC AAAGTGTGCT GTATGAATCC CTGCAGCAGC ATCCATTGGC CAAGTACCAT 
CAGACGCAGC AGCAAACGAC AACGACGACG ACGGTGGATG GACAGACGAG TAGCCGGATG 
GAGCTGCACA AGGCGACCCT GGCGAATGCC ACCATCGATC CGGTGCCAGT TGGATCCAGC 
AAGTTTGTGA CGCTGCCGGA TAAGCCGGAT AAGGGTTCCT CCGTTGCCAC CTTGGCGGAC 
ATCGCCGAGA GCAGTGCAGC AGCCATTGTG GACAGTGCGT CGGGAAGCGG CAACGTTGTG 
CGGCGCTACA AGCCATTATC CTCCAGCTCC TTTGACGAAG GCAAACCAAT GCGTAGGATA 
TCCTACCTGC GGGCAACCAA CAATGAGACC GACTTCAATG CGGAGGCTGC CGCTGGCGGA 
GATGCCGCGG CAACAGGGGC GTCAGGACCA GCATCTGCAT CAGCAGCTTC ATCTGCACCT 
GGACCCACGC CCTCATCTGC TCCTGCACCA GCTCCAGCAC CAGCACCCCT GCCCATTTCG 
ATCCCCATTC CGGTGGCTGC TGTCGTCGAG CCGCAAAAGA CGAAGTCCCT GGTGGAGGAG 
CATCCGGATC CCACCTACGA TGTGATCGGA GGCACAGGTG GTGGCCACGA GTTCATCGAG 
ACGCCGAACG GCCTGGAGGA TGTGCGTCTG TCGGCCCACG GGAGTCGGAG GGCCTCCATG 
AGCAGTGACA TGCGAAGCAG CATCCACATT GATGCCGAGT TAAATGGCAA GTATGTGCCA 
AACGAACTGG AGGCCTTTGA GACGGAAATC GAGATCAAGT CCTCTTGCAT CAACGGAAAA 
CGCATGTCCG AGTACCGATC ATGGCGTCAG GTGAAGCTGG AGATCAAGGG AGATCTGCTG 
CGTATCTACT CAGGCCGACA TGCCAAATCG GAGCACAATG TGATAGAACT TGATATTAGA 
AACTTTAAGT TCTTCGACGA GTCGATGGAT AAGAAGAAAT ACTTGATCCG TATGCAGTCG 
AAGCCATCGC CCAGTGGAGC CCATTTGGCC TCCTTGGGCA ACGAGTTAAA CCTGGACGAG 
GGTCACGACA AGCTGCTAAC CTCGTCGGGC AGCAGCAGTG CGAATGAGCC CGCTGCCAAC 
GCAGCATCGG CCCTGACCGC ATCCACCTCC TCGTCCACCT GCAGCAGCGG TCCTTACACG 
GAGATCCTAT TCAAGACGAA GAGCAGCAAC GAGATGAAGC GTCTGTTTGG ACTTCTGCAG 
TGGAAGGATA GCCTCAACTA CGATGATAAT GAGCACCAGC CACAGGGCAA GCAGTTGGCA 
GAACCACAGA AAACAGTGGC CAGCGCCAGT TATCTGGACG ACGTCCAAGG TGGAGCGAGT 
GCAGCCGACA GCTCACCCTT GAGCTCCCAT TCCGGTGGCA TTGCAGACGC TAGTGGCAGT 
CATCATGTTT CTGCGCTACC AGCAGCTGCG GCTATTGTCG CCGCCACCAG CGACTCCATT 
TCGCCGGTGA TGAAGGCAAG GAAGTCCTCT TCGCACAAGC ATATACCTGA TAAGGATTTG 
GGATCGCCCA AATCGAAGAA CTGGAAGGAT TTGCTCTTCC GCCGTGGCGG CAGTGGCAGC 
GGAGGAGTGT CACACCACGA CTTGGCTTCG CCATCCGCCT GTGCAGCCAA GCAGATTGGA 
TCTATTGGCG TTCCACTGCG CAGCTGTCCC ATGTCGAAGG TCAATGCATA TGTGCCGCAT 
CTGGTAGAGG TGTGCACGAA CATTGTGGAG ACAAAGGGTC TGGGAGTGGT GGGCATCTAT 
CGCATACCCG GCAACAAGGC GGCCATATCA GAGCTGTCCG AACTTGTTAA CACCAAGGAC 
TTTCAGTTCG AGAGCTGTGC CAGCGATGAC CGCTGGGAGG ATGTGAATGT GGTGAGCAGC 
CTGCTCAAGT TGTTTATACG CAGCTTGCCC GATGCCTTAA TGCCGGCAAG TTACTATATC 
AACTTTATTG AGGCGGACAA GAAGTTTGGC CTGGAGAGAA TTGTACTGTT GCGCGAGATT 
GTGGAATCGT TACCACGGCA TCCCTACGAA ACGATGAAGC ACTTGATTCG GCATCTGTGC 
CGAGTTAGCG GCAATTGCGA TGTGAACCGC ATGGAGCCCA AGAACCTGGC CATCATATTT 
GGGCCATCTA TAATCAGGAC GCCGAACGAT ACTCTGGAAA CGGCAGTCAA GGACATGAAG 
CATCAATGCC GCATCGTTGA GCTGCTGGTC ACACAGTACG ACTACTTCTT CGAAGGCGGC 
AGTCTGCCGG ATATAGCCGA AGTAAGCGGC GGTACAGCTG CCGTAAGCGC CGCTCAGCCG 
CAGCAGCAGA CGCAGGAGGA TCAGACGAGC ATGCTGCTGC ACAACCTGTC GAAGATCGAG 
CGGATCACAG AGCGCGAAAC CACGCGCACC TCGCGCTTTA TGCCGCAGCT CAGACGAAAG 
ACCCATGGCA AGCGCAGCGC CGTAAACTCG GACACGTACT CCGGCGAGAG CGTTCTGTCG 
TTGGACTACG CCAAGGCGTC CGCCTCTGCC TCGGCATCCG CAAGCAGCAC CGCCAGTACT 
TCGAGCAGCT CGACGCTTCA CAGCGGGGGC AAGGCATCCG CCTCATCCTC ATCCAGTTCC 
ACTACCAAGT TCAGCTCATC GTCATCGTCG GCGTGCTCAA CAACGGCGGT GGCTATTTCT 
TCCTCCATCT CGGCGACGTC CTCCAAATTG TTAAGCGCCA ATTTTAAGAA GCGTAGCACA 
GGAGGTTTCT TGGGCTCAAG ATCCTCGCAC CAGACGCGCA AGGCCAGCTT GGACGAAAAG 
GACTCAGGAC AGTCGTCTGG CAGCCTTGGT CACAGCCTGG GAGTAGGCAT AAATCTGGCC 
AAGGAACAAC AGCGCAGCAG CGTGGACATA TCCATTCTGC TAACGGACGA CGACTCTAGC 
AGCAGGCTAA GCGATACGGG TTCCATGTCG CTGACGACCA TTACGGATAC ACTGGACAGC 
AAGCTACGCA ACTTGCGCAG CGGCTCCGAA TCGAATGATG AGAATGGATC GCCCGAGTTT 
CCCAAGAACC GGCGGCACAC CTTGGGCCAG CCTCTGCACA TGCACTCCGA GAACATTCCG 
TATGCGGACG AGTCGCCCGA GCGGCTGTTC CATCAGTTTT GCAATCCCCT GGAGACAGCC 
CCCTTATCCC TGCATCTAAG CCGCTCCTGC ACGGCCACAA ATACAATTGG TGGCGATGAA 
AAGCCTTCGA AACAGTCGGG TGGAGTTGCA CCACTGCCCC CAAGTTTGCT TAAGGCCGCA 
CATAAGCTAC AGCACTCAGC CACGGTGCCC ATTCATCAGG GAAGCTCCAC TGCACCAACT 
AGCGGAGCAG CCACGCCAGT AGGCACGCCA ACCGGAAGCG GTGGGGCCGG AGCTATTCCC 
TCGCGCAGCT CATCGAGCAG TGTCACGACG CTAACCAAAA GCATACAACC CAGGTTCAGT 
AACTATCTAA GCTACGAAAG AGGCAATGCA GCCGCTGGAG GCGCCGAAAG GGTGGTGGGA 
TCCGCCGGAG GAGCTGCCTC CGACGACGAT TCCGAGGGCT CGACCACCAG TGATCCCAAG 
GAGAAGCTGC TTCTCGGTGA ACGACCGTCG CCTTCGTTTT TTATCGAGCG CTACAACAAA 
AAGAGACGCG ATCATCGGTT ATTTCGCAGC GCCAGTTTCA ATTGCCGGAA CTACTCCACC 
AGGCACATAA CGGCGCAGAC ATCCGGAGGA GTAGCGGCGG GAGTATCGGG CGGAGTAGCT 
GGAGCGGTGG CGGTGGGGGT GGCTTGCTGC CAGGCATTGA GCACCACTGG CTTGACCAAG 
GACGAGAAGA CTGATATGAA CCTGACCAAG AAGCGCCAGA TCCAGAACAA GCAGAATCGG 
TCCATTAAGC GGCGGCACAC AGTAGGCGGA CCTCACGACT ATTCCGCCAG CAATGGTGGC 
TGCTCGAATC ACTCACACGG TCACGACCTG GCAGCCATCA ACTACAATCA CCACAATCGC 
CATCACCAGC AACAGTTGTT GAAACTGGGC AGCGCTACAT CTGGCGGTGG AGGCAGTGGC 
GCAGCCGCGG CCGAGACGAC GACCACCACA ACGACCACCA CCACGGTGCT GCGGCGCCTC 
GGTACAGGTC AAGCTGGCGC CGAAGCTAGT GTGGCATTGC CCGCATCAAG TTCCGGATCG 
AGCAGCAACA ACAATAACTC GCCCCAAAGC GATGGCAGTA ATGGCAACGG CGGTGGAGTG 
GCGGGAGTTG CCTCCAATGC ACCACCAACT GGCGTAGGTG TCGGTGGCAA CAACAGCAGC 
GGAAGCAGCA GCAACAGCAG CAGCACCAGC ACAACTCCCG CTGGGAACGG CGATCAGGTC 
CTGGAAGTGG GCATACGTAT CAAGAACATT AGCCGGGGTC GCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RhoGAP19D ( NM_001298568.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285497.1 Drosophila melanogaster Rho GTPase activating protein at 19D (RhoGAP19D), transcript variant E, mRNA.

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CloneID	OFa02209
Clone ID Related Accession (Same CDS sequence)	NM_001258851.1 , NM_001298568.1
Accession Version	NM_001298568.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho GTPase activating protein at 19D, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTACAAA ACTCGAACGG CGCTGCCGCA GCGCATTCGG CAGCCTCAGC GGTGGCGACT 
CCGGCAGCGG CGGCATCCGC AGCAGCAGCA GCTGCTGCAG CGGCGGTGGC TGCAGCGGCC 
AACAACGCCG CCCTGGCCGC CTCCTCCATC CAGCCCAAGC TCATCGTCAT CCGCCGGCGA 
CCCAACCAGG GATTCGGGTT CACGCTGCGC CACTTCATCG CCTATCCGCC GGAAGATGAC 
CAGGCATCAT CATCCGCATC GGGTTTGGTA TCGGGATCGG CGACAGCAGC AACGGCGGCA 
TCGGTATCGA CCAACTGGCC ACAGGAAGCC TCCAGTGCGG CGGGCAGCAA CAGCGGCAGC 
AGCAGCAGCG TCGGCGTCGC TGGGATCACC GGTTTGGAGC CAACGTCGCC GACGTCGCTG 
CCGCCGTACC AAGTGAAAGC GATGGAGACC ATTTTCATCA AAGAGGTGCA GGCGAACGGA 
CCGGCCCACT ACGCCAACCT GCAGACGGGC GACCGCGTGC TGATGGTCAA CAACCAGCCG 
ATCGCTGGCA TCGCCTACAG CACCATCGTC TCGATGATCA AGCAGACGCC GGCGGTCCTC 
ACGCTGCACG TGGTGCCCAA GGAGTGCGAT GTCCTGCAGA TGCACTATAC GAGCATTGCC 
CACACGCCGG AGTCGAATCG CCTGACCATG TCGACGCACT CGCCCTCGCT GCTGGCCAAC 
GGCTCCCACA AGACCACATT TCCAGCGGCG GCAGCAGTTG CAGCACCACC GCATCAGCAT 
CAGCAGCAGC AGCAACAGTT GATCTCATAT CCCGCTGTTG CAGCCGTGAC CACATCAACG 
CCGGCAGGAT CGCCGCAATC CCAATCCCAA TCCCAATCGC ACTCGCACCC CAACACCCAT 
CATGCACCAT CCCTGCACGG AGGCAGTCGA TCGGGCAGCA TCACCAGCAC GGCCAGCGGA 
GGCATCACCG TCCTCAGTCA GCCGTTCTAC CCACAGCAGC AGCAGCAACA GCAACAGCAG 
CAGCAACATC GTCATCCAGC GCTGACTGGC GGCAGCAGCA GCATCGATTT CGGGGACATG 
GCACATGGAC TGCGCCAGCA TCAGCAGCAG CAGCAACATC TGTACCAGCA GCAGCAACAG 
CATCACCAGT TTATGCGTGC TAATCAGCCG CCAAATTTAA CAGTATTATC GGCCAGCTCG 
GCAACGAACA CGCCCACACC CACCGCCCGC CAGCCCAAGT CCGCGACGAG CCACAATCAG 
GCGTTGTATG CAGTCGGAGC GGGTCCGAGC TCAGCAGCTG GATCTGGGTC GGGATCCCTT 
TCGGGGGGCA GTGAGAACAG CGACCTTATG ATCCGACTGC GCGAGTCCAT CAAGCAGAAG 
GAGGAGTTCC TTAAGTCGCC GGTGCCCGCC TCCATGAGCT CCGCCTCCTC TATCAACGGC 
AACCAGGCGC AGTCGTCATC GCCGGCACAG CAGCAACACC TGCAGCAGCA CCAGCAGCAC 
TTCTTTCCCT CGCACACGCC GCCAGGTGGT GTCCAAGTGG TGGTGCCTCC ACCGCGCAGT 
CGCCATCAAG TGATGCTCAA GCAGCAGCAG CAGCAACAGC AGCAGCACCA GCAACATCAT 
GTCCTTGGCT ACGACATGTA CCACTCCGGT GGTAAGCTGG TGCAGCGGTC TTCCACGGCG 
CCTGGCAGTG TGTGCCACCA ACACCAGCAG CAGCAGCAGC ATCACCTCTA TCACCAGCAG 
CAACATCAGC AGCTCCAGCA GACGCAACAT CGCCAGGTGA TGATCAACAA CAATGCCAAG 
TACGCCAAGG ATCTGGACTA TTTTGAGACG TTGCAGGAGA CAGGAGTCAC CAATAAGGTC 
TTTGAGCGCA AGCTGGTGTC ACACATGTCC AAGGGATTCG ATCCGTTTAA GCCGCTGTCG 
ATCAACACCA GTGCCCTGGA GTCGTCTGCC AGCACGTCGA GCACTTCGGC CACCACGACG 
GTGAAGAAGC AAAGTGTGCT GTATGAATCC CTGCAGCAGC ATCCATTGGC CAAGTACCAT 
CAGACGCAGC AGCAAACGAC AACGACGACG ACGGTGGATG GACAGACGAG TAGCCGGATG 
GAGCTGCACA AGGCGACCCT GGCGAATGCC ACCATCGATC CGGTGCCAGT TGGATCCAGC 
AAGTTTGTGA CGCTGCCGGA TAAGCCGGAT AAGGGTTCCT CCGTTGCCAC CTTGGCGGAC 
ATCGCCGAGA GCAGTGCAGC AGCCATTGTG GACAGTGCGT CGGGAAGCGG CAACGTTGTG 
CGGCGCTACA AGCCATTATC CTCCAGCTCC TTTGACGAAG GCAAACCAAT GCGTAGGATA 
TCCTACCTGC GGGCAACCAA CAATGAGACC GACTTCAATG CGGAGGCTGC CGCTGGCGGA 
GATGCCGCGG CAACAGGGGC GTCAGGACCA GCATCTGCAT CAGCAGCTTC ATCTGCACCT 
GGACCCACGC CCTCATCTGC TCCTGCACCA GCTCCAGCAC CAGCACCCCT GCCCATTTCG 
ATCCCCATTC CGGTGGCTGC TGTCGTCGAG CCGCAAAAGA CGAAGTCCCT GGTGGAGGAG 
CATCCGGATC CCACCTACGA TGTGATCGGA GGCACAGGTG GTGGCCACGA GTTCATCGAG 
ACGCCGAACG GCCTGGAGGA TGTGCGTCTG TCGGCCCACG GGAGTCGGAG GGCCTCCATG 
AGCAGTGACA TGCGAAGCAG CATCCACATT GATGCCGAGT TAAATGGCAA GTATGTGCCA 
AACGAACTGG AGGCCTTTGA GACGGAAATC GAGATCAAGT CCTCTTGCAT CAACGGAAAA 
CGCATGTCCG AGTACCGATC ATGGCGTCAG GTGAAGCTGG AGATCAAGGG AGATCTGCTG 
CGTATCTACT CAGGCCGACA TGCCAAATCG GAGCACAATG TGATAGAACT TGATATTAGA 
AACTTTAAGT TCTTCGACGA GTCGATGGAT AAGAAGAAAT ACTTGATCCG TATGCAGTCG 
AAGCCATCGC CCAGTGGAGC CCATTTGGCC TCCTTGGGCA ACGAGTTAAA CCTGGACGAG 
GGTCACGACA AGCTGCTAAC CTCGTCGGGC AGCAGCAGTG CGAATGAGCC CGCTGCCAAC 
GCAGCATCGG CCCTGACCGC ATCCACCTCC TCGTCCACCT GCAGCAGCGG TCCTTACACG 
GAGATCCTAT TCAAGACGAA GAGCAGCAAC GAGATGAAGC GTCTGTTTGG ACTTCTGCAG 
TGGAAGGATA GCCTCAACTA CGATGATAAT GAGCACCAGC CACAGGGCAA GCAGTTGGCA 
GAACCACAGA AAACAGTGGC CAGCGCCAGT TATCTGGACG ACGTCCAAGG TGGAGCGAGT 
GCAGCCGACA GCTCACCCTT GAGCTCCCAT TCCGGTGGCA TTGCAGACGC TAGTGGCAGT 
CATCATGTTT CTGCGCTACC AGCAGCTGCG GCTATTGTCG CCGCCACCAG CGACTCCATT 
TCGCCGGTGA TGAAGGCAAG GAAGTCCTCT TCGCACAAGC ATATACCTGA TAAGGATTTG 
GGATCGCCCA AATCGAAGAA CTGGAAGGAT TTGCTCTTCC GCCGTGGCGG CAGTGGCAGC 
GGAGGAGTGT CACACCACGA CTTGGCTTCG CCATCCGCCT GTGCAGCCAA GCAGATTGGA 
TCTATTGGCG TTCCACTGCG CAGCTGTCCC ATGTCGAAGG TCAATGCATA TGTGCCGCAT 
CTGGTAGAGG TGTGCACGAA CATTGTGGAG ACAAAGGGTC TGGGAGTGGT GGGCATCTAT 
CGCATACCCG GCAACAAGGC GGCCATATCA GAGCTGTCCG AACTTGTTAA CACCAAGGAC 
TTTCAGTTCG AGAGCTGTGC CAGCGATGAC CGCTGGGAGG ATGTGAATGT GGTGAGCAGC 
CTGCTCAAGT TGTTTATACG CAGCTTGCCC GATGCCTTAA TGCCGGCAAG TTACTATATC 
AACTTTATTG AGGCGGACAA GAAGTTTGGC CTGGAGAGAA TTGTACTGTT GCGCGAGATT 
GTGGAATCGT TACCACGGCA TCCCTACGAA ACGATGAAGC ACTTGATTCG GCATCTGTGC 
CGAGTTAGCG GCAATTGCGA TGTGAACCGC ATGGAGCCCA AGAACCTGGC CATCATATTT 
GGGCCATCTA TAATCAGGAC GCCGAACGAT ACTCTGGAAA CGGCAGTCAA GGACATGAAG 
CATCAATGCC GCATCGTTGA GCTGCTGGTC ACACAGTACG ACTACTTCTT CGAAGGCGGC 
AGTCTGCCGG ATATAGCCGA AGTAAGCGGC GGTACAGCTG CCGTAAGCGC CGCTCAGCCG 
CAGCAGCAGA CGCAGGAGGA TCAGACGAGC ATGCTGCTGC ACAACCTGTC GAAGATCGAG 
CGGATCACAG AGCGCGAAAC CACGCGCACC TCGCGCTTTA TGCCGCAGCT CAGACGAAAG 
ACCCATGGCA AGCGCAGCGC CGTAAACTCG GACACGTACT CCGGCGAGAG CGTTCTGTCG 
TTGGACTACG CCAAGGCGTC CGCCTCTGCC TCGGCATCCG CAAGCAGCAC CGCCAGTACT 
TCGAGCAGCT CGACGCTTCA CAGCGGGGGC AAGGCATCCG CCTCATCCTC ATCCAGTTCC 
ACTACCAAGT TCAGCTCATC GTCATCGTCG GCGTGCTCAA CAACGGCGGT GGCTATTTCT 
TCCTCCATCT CGGCGACGTC CTCCAAATTG TTAAGCGCCA ATTTTAAGAA GCGTAGCACA 
GGAGGTTTCT TGGGCTCAAG ATCCTCGCAC CAGACGCGCA AGGCCAGCTT GGACGAAAAG 
GACTCAGGAC AGTCGTCTGG CAGCCTTGGT CACAGCCTGG GAGTAGGCAT AAATCTGGCC 
AAGGAACAAC AGCGCAGCAG CGTGGACATA TCCATTCTGC TAACGGACGA CGACTCTAGC 
AGCAGGCTAA GCGATACGGG TTCCATGTCG CTGACGACCA TTACGGATAC ACTGGACAGC 
AAGCTACGCA ACTTGCGCAG CGGCTCCGAA TCGAATGATG AGAATGGATC GCCCGAGTTT 
CCCAAGAACC GGCGGCACAC CTTGGGCCAG CCTCTGCACA TGCACTCCGA GAACATTCCG 
TATGCGGACG AGTCGCCCGA GCGGCTGTTC CATCAGTTTT GCAATCCCCT GGAGACAGCC 
CCCTTATCCC TGCATCTAAG CCGCTCCTGC ACGGCCACAA ATACAATTGG TGGCGATGAA 
AAGCCTTCGA AACAGTCGGG TGGAGTTGCA CCACTGCCCC CAAGTTTGCT TAAGGCCGCA 
CATAAGCTAC AGCACTCAGC CACGGTGCCC ATTCATCAGG GAAGCTCCAC TGCACCAACT 
AGCGGAGCAG CCACGCCAGT AGGCACGCCA ACCGGAAGCG GTGGGGCCGG AGCTATTCCC 
TCGCGCAGCT CATCGAGCAG TGTCACGACG CTAACCAAAA GCATACAACC CAGGTTCAGT 
AACTATCTAA GCTACGAAAG AGGCAATGCA GCCGCTGGAG GCGCCGAAAG GGTGGTGGGA 
TCCGCCGGAG GAGCTGCCTC CGACGACGAT TCCGAGGGCT CGACCACCAG TGATCCCAAG 
GAGAAGCTGC TTCTCGGTGA ACGACCGTCG CCTTCGTTTT TTATCGAGCG CTACAACAAA 
AAGAGACGCG ATCATCGGTT ATTTCGCAGC GCCAGTTTCA ATTGCCGGAA CTACTCCACC 
AGGCACATAA CGGCGCAGAC ATCCGGAGGA GTAGCGGCGG GAGTATCGGG CGGAGTAGCT 
GGAGCGGTGG CGGTGGGGGT GGCTTGCTGC CAGGCATTGA GCACCACTGG CTTGACCAAG 
GACGAGAAGA CTGATATGAA CCTGACCAAG AAGCGCCAGA TCCAGAACAA GCAGAATCGG 
TCCATTAAGC GGCGGCACAC AGTAGGCGGA CCTCACGACT ATTCCGCCAG CAATGGTGGC 
TGCTCGAATC ACTCACACGG TCACGACCTG GCAGCCATCA ACTACAATCA CCACAATCGC 
CATCACCAGC AACAGTTGTT GAAACTGGGC AGCGCTACAT CTGGCGGTGG AGGCAGTGGC 
GCAGCCGCGG CCGAGACGAC GACCACCACA ACGACCACCA CCACGGTGCT GCGGCGCCTC 
GGTACAGGTC AAGCTGGCGC CGAAGCTAGT GTGGCATTGC CCGCATCAAG TTCCGGATCG 
AGCAGCAACA ACAATAACTC GCCCCAAAGC GATGGCAGTA ATGGCAACGG CGGTGGAGTG 
GCGGGAGTTG CCTCCAATGC ACCACCAACT GGCGTAGGTG TCGGTGGCAA CAACAGCAGC 
GGAAGCAGCA GCAACAGCAG CAGCACCAGC ACAACTCCCG CTGGGAACGG CGATCAGGTC 
CTGGAAGTGG GCATACGTAT CAAGAACATT AGCCGGGGTC GCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285497.1
CDS	318..6725
Translation	MLQNSNGAAAAHSAASAVATPAAAASAAAAAAAAAVAAAANNAALAASSIQPKLIVIRRRPNQGFGFTLRHFIAYPPEDDQASSSASGLVSGSATAATAASVSTNWPQEASSAAGSNSGSSSSVGVAGITGLEPTSPTSLPPYQVKAMETIFIKEVQANGPAHYANLQTGDRVLMVNNQPIAGIAYSTIVSMIKQTPAVLTLHVVPKECDVLQMHYTSIAHTPESNRLTMSTHSPSLLANGSHKTTFPAAAAVAAPPHQHQQQQQQLISYPAVAAVTTSTPAGSPQSQSQSQSHSHPNTHHAPSLHGGSRSGSITSTASGGITVLSQPFYPQQQQQQQQQQQHRHPALTGGSSSIDFGDMAHGLRQHQQQQQHLYQQQQQHHQFMRANQPPNLTVLSASSATNTPTPTARQPKSATSHNQALYAVGAGPSSAAGSGSGSLSGGSENSDLMIRLRESIKQKEEFLKSPVPASMSSASSINGNQAQSSSPAQQQHLQQHQQHFFPSHTPPGGVQVVVPPPRSRHQVMLKQQQQQQQQHQQHHVLGYDMYHSGGKLVQRSSTAPGSVCHQHQQQQQHHLYHQQQHQQLQQTQHRQVMINNNAKYAKDLDYFETLQETGVTNKVFERKLVSHMSKGFDPFKPLSINTSALESSASTSSTSATTTVKKQSVLYESLQQHPLAKYHQTQQQTTTTTTVDGQTSSRMELHKATLANATIDPVPVGSSKFVTLPDKPDKGSSVATLADIAESSAAAIVDSASGSGNVVRRYKPLSSSSFDEGKPMRRISYLRATNNETDFNAEAAAGGDAAATGASGPASASAASSAPGPTPSSAPAPAPAPAPLPISIPIPVAAVVEPQKTKSLVEEHPDPTYDVIGGTGGGHEFIETPNGLEDVRLSAHGSRRASMSSDMRSSIHIDAELNGKYVPNELEAFETEIEIKSSCINGKRMSEYRSWRQVKLEIKGDLLRIYSGRHAKSEHNVIELDIRNFKFFDESMDKKKYLIRMQSKPSPSGAHLASLGNELNLDEGHDKLLTSSGSSSANEPAANAASALTASTSSSTCSSGPYTEILFKTKSSNEMKRLFGLLQWKDSLNYDDNEHQPQGKQLAEPQKTVASASYLDDVQGGASAADSSPLSSHSGGIADASGSHHVSALPAAAAIVAATSDSISPVMKARKSSSHKHIPDKDLGSPKSKNWKDLLFRRGGSGSGGVSHHDLASPSACAAKQIGSIGVPLRSCPMSKVNAYVPHLVEVCTNIVETKGLGVVGIYRIPGNKAAISELSELVNTKDFQFESCASDDRWEDVNVVSSLLKLFIRSLPDALMPASYYINFIEADKKFGLERIVLLREIVESLPRHPYETMKHLIRHLCRVSGNCDVNRMEPKNLAIIFGPSIIRTPNDTLETAVKDMKHQCRIVELLVTQYDYFFEGGSLPDIAEVSGGTAAVSAAQPQQQTQEDQTSMLLHNLSKIERITERETTRTSRFMPQLRRKTHGKRSAVNSDTYSGESVLSLDYAKASASASASASSTASTSSSSTLHSGGKASASSSSSSTTKFSSSSSSACSTTAVAISSSISATSSKLLSANFKKRSTGGFLGSRSSHQTRKASLDEKDSGQSSGSLGHSLGVGINLAKEQQRSSVDISILLTDDDSSSRLSDTGSMSLTTITDTLDSKLRNLRSGSESNDENGSPEFPKNRRHTLGQPLHMHSENIPYADESPERLFHQFCNPLETAPLSLHLSRSCTATNTIGGDEKPSKQSGGVAPLPPSLLKAAHKLQHSATVPIHQGSSTAPTSGAATPVGTPTGSGGAGAIPSRSSSSSVTTLTKSIQPRFSNYLSYERGNAAAGGAERVVGSAGGAASDDDSEGSTTSDPKEKLLLGERPSPSFFIERYNKKRRDHRLFRSASFNCRNYSTRHITAQTSGGVAAGVSGGVAGAVAVGVACCQALSTTGLTKDEKTDMNLTKKRQIQNKQNRSIKRRHTVGGPHDYSASNGGCSNHSHGHDLAAINYNHHNRHHQQQLLKLGSATSGGGGSGAAAAETTTTTTTTTTVLRRLGTGQAGAEASVALPASSSGSSSNNNNSPQSDGSNGNGGGVAGVASNAPPTGVGVGGNNSSGSSSNSSSTSTTPAGNGDQVLEVGIRIKNISRGRF

Target ORF information:

RefSeq Version	NM_001298568.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho GTPase activating protein at 19D (RhoGAP19D), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298568.1

ORF Insert Sequence:

ATGTTACAAA ACTCGAACGG CGCTGCCGCA GCGCATTCGG CAGCCTCAGC GGTGGCGACT 
CCGGCAGCGG CGGCATCCGC AGCAGCAGCA GCTGCTGCAG CGGCGGTGGC TGCAGCGGCC 
AACAACGCCG CCCTGGCCGC CTCCTCCATC CAGCCCAAGC TCATCGTCAT CCGCCGGCGA 
CCCAACCAGG GATTCGGGTT CACGCTGCGC CACTTCATCG CCTATCCGCC GGAAGATGAC 
CAGGCATCAT CATCCGCATC GGGTTTGGTA TCGGGATCGG CGACAGCAGC AACGGCGGCA 
TCGGTATCGA CCAACTGGCC ACAGGAAGCC TCCAGTGCGG CGGGCAGCAA CAGCGGCAGC 
AGCAGCAGCG TCGGCGTCGC TGGGATCACC GGTTTGGAGC CAACGTCGCC GACGTCGCTG 
CCGCCGTACC AAGTGAAAGC GATGGAGACC ATTTTCATCA AAGAGGTGCA GGCGAACGGA 
CCGGCCCACT ACGCCAACCT GCAGACGGGC GACCGCGTGC TGATGGTCAA CAACCAGCCG 
ATCGCTGGCA TCGCCTACAG CACCATCGTC TCGATGATCA AGCAGACGCC GGCGGTCCTC 
ACGCTGCACG TGGTGCCCAA GGAGTGCGAT GTCCTGCAGA TGCACTATAC GAGCATTGCC 
CACACGCCGG AGTCGAATCG CCTGACCATG TCGACGCACT CGCCCTCGCT GCTGGCCAAC 
GGCTCCCACA AGACCACATT TCCAGCGGCG GCAGCAGTTG CAGCACCACC GCATCAGCAT 
CAGCAGCAGC AGCAACAGTT GATCTCATAT CCCGCTGTTG CAGCCGTGAC CACATCAACG 
CCGGCAGGAT CGCCGCAATC CCAATCCCAA TCCCAATCGC ACTCGCACCC CAACACCCAT 
CATGCACCAT CCCTGCACGG AGGCAGTCGA TCGGGCAGCA TCACCAGCAC GGCCAGCGGA 
GGCATCACCG TCCTCAGTCA GCCGTTCTAC CCACAGCAGC AGCAGCAACA GCAACAGCAG 
CAGCAACATC GTCATCCAGC GCTGACTGGC GGCAGCAGCA GCATCGATTT CGGGGACATG 
GCACATGGAC TGCGCCAGCA TCAGCAGCAG CAGCAACATC TGTACCAGCA GCAGCAACAG 
CATCACCAGT TTATGCGTGC TAATCAGCCG CCAAATTTAA CAGTATTATC GGCCAGCTCG 
GCAACGAACA CGCCCACACC CACCGCCCGC CAGCCCAAGT CCGCGACGAG CCACAATCAG 
GCGTTGTATG CAGTCGGAGC GGGTCCGAGC TCAGCAGCTG GATCTGGGTC GGGATCCCTT 
TCGGGGGGCA GTGAGAACAG CGACCTTATG ATCCGACTGC GCGAGTCCAT CAAGCAGAAG 
GAGGAGTTCC TTAAGTCGCC GGTGCCCGCC TCCATGAGCT CCGCCTCCTC TATCAACGGC 
AACCAGGCGC AGTCGTCATC GCCGGCACAG CAGCAACACC TGCAGCAGCA CCAGCAGCAC 
TTCTTTCCCT CGCACACGCC GCCAGGTGGT GTCCAAGTGG TGGTGCCTCC ACCGCGCAGT 
CGCCATCAAG TGATGCTCAA GCAGCAGCAG CAGCAACAGC AGCAGCACCA GCAACATCAT 
GTCCTTGGCT ACGACATGTA CCACTCCGGT GGTAAGCTGG TGCAGCGGTC TTCCACGGCG 
CCTGGCAGTG TGTGCCACCA ACACCAGCAG CAGCAGCAGC ATCACCTCTA TCACCAGCAG 
CAACATCAGC AGCTCCAGCA GACGCAACAT CGCCAGGTGA TGATCAACAA CAATGCCAAG 
TACGCCAAGG ATCTGGACTA TTTTGAGACG TTGCAGGAGA CAGGAGTCAC CAATAAGGTC 
TTTGAGCGCA AGCTGGTGTC ACACATGTCC AAGGGATTCG ATCCGTTTAA GCCGCTGTCG 
ATCAACACCA GTGCCCTGGA GTCGTCTGCC AGCACGTCGA GCACTTCGGC CACCACGACG 
GTGAAGAAGC AAAGTGTGCT GTATGAATCC CTGCAGCAGC ATCCATTGGC CAAGTACCAT 
CAGACGCAGC AGCAAACGAC AACGACGACG ACGGTGGATG GACAGACGAG TAGCCGGATG 
GAGCTGCACA AGGCGACCCT GGCGAATGCC ACCATCGATC CGGTGCCAGT TGGATCCAGC 
AAGTTTGTGA CGCTGCCGGA TAAGCCGGAT AAGGGTTCCT CCGTTGCCAC CTTGGCGGAC 
ATCGCCGAGA GCAGTGCAGC AGCCATTGTG GACAGTGCGT CGGGAAGCGG CAACGTTGTG 
CGGCGCTACA AGCCATTATC CTCCAGCTCC TTTGACGAAG GCAAACCAAT GCGTAGGATA 
TCCTACCTGC GGGCAACCAA CAATGAGACC GACTTCAATG CGGAGGCTGC CGCTGGCGGA 
GATGCCGCGG CAACAGGGGC GTCAGGACCA GCATCTGCAT CAGCAGCTTC ATCTGCACCT 
GGACCCACGC CCTCATCTGC TCCTGCACCA GCTCCAGCAC CAGCACCCCT GCCCATTTCG 
ATCCCCATTC CGGTGGCTGC TGTCGTCGAG CCGCAAAAGA CGAAGTCCCT GGTGGAGGAG 
CATCCGGATC CCACCTACGA TGTGATCGGA GGCACAGGTG GTGGCCACGA GTTCATCGAG 
ACGCCGAACG GCCTGGAGGA TGTGCGTCTG TCGGCCCACG GGAGTCGGAG GGCCTCCATG 
AGCAGTGACA TGCGAAGCAG CATCCACATT GATGCCGAGT TAAATGGCAA GTATGTGCCA 
AACGAACTGG AGGCCTTTGA GACGGAAATC GAGATCAAGT CCTCTTGCAT CAACGGAAAA 
CGCATGTCCG AGTACCGATC ATGGCGTCAG GTGAAGCTGG AGATCAAGGG AGATCTGCTG 
CGTATCTACT CAGGCCGACA TGCCAAATCG GAGCACAATG TGATAGAACT TGATATTAGA 
AACTTTAAGT TCTTCGACGA GTCGATGGAT AAGAAGAAAT ACTTGATCCG TATGCAGTCG 
AAGCCATCGC CCAGTGGAGC CCATTTGGCC TCCTTGGGCA ACGAGTTAAA CCTGGACGAG 
GGTCACGACA AGCTGCTAAC CTCGTCGGGC AGCAGCAGTG CGAATGAGCC CGCTGCCAAC 
GCAGCATCGG CCCTGACCGC ATCCACCTCC TCGTCCACCT GCAGCAGCGG TCCTTACACG 
GAGATCCTAT TCAAGACGAA GAGCAGCAAC GAGATGAAGC GTCTGTTTGG ACTTCTGCAG 
TGGAAGGATA GCCTCAACTA CGATGATAAT GAGCACCAGC CACAGGGCAA GCAGTTGGCA 
GAACCACAGA AAACAGTGGC CAGCGCCAGT TATCTGGACG ACGTCCAAGG TGGAGCGAGT 
GCAGCCGACA GCTCACCCTT GAGCTCCCAT TCCGGTGGCA TTGCAGACGC TAGTGGCAGT 
CATCATGTTT CTGCGCTACC AGCAGCTGCG GCTATTGTCG CCGCCACCAG CGACTCCATT 
TCGCCGGTGA TGAAGGCAAG GAAGTCCTCT TCGCACAAGC ATATACCTGA TAAGGATTTG 
GGATCGCCCA AATCGAAGAA CTGGAAGGAT TTGCTCTTCC GCCGTGGCGG CAGTGGCAGC 
GGAGGAGTGT CACACCACGA CTTGGCTTCG CCATCCGCCT GTGCAGCCAA GCAGATTGGA 
TCTATTGGCG TTCCACTGCG CAGCTGTCCC ATGTCGAAGG TCAATGCATA TGTGCCGCAT 
CTGGTAGAGG TGTGCACGAA CATTGTGGAG ACAAAGGGTC TGGGAGTGGT GGGCATCTAT 
CGCATACCCG GCAACAAGGC GGCCATATCA GAGCTGTCCG AACTTGTTAA CACCAAGGAC 
TTTCAGTTCG AGAGCTGTGC CAGCGATGAC CGCTGGGAGG ATGTGAATGT GGTGAGCAGC 
CTGCTCAAGT TGTTTATACG CAGCTTGCCC GATGCCTTAA TGCCGGCAAG TTACTATATC 
AACTTTATTG AGGCGGACAA GAAGTTTGGC CTGGAGAGAA TTGTACTGTT GCGCGAGATT 
GTGGAATCGT TACCACGGCA TCCCTACGAA ACGATGAAGC ACTTGATTCG GCATCTGTGC 
CGAGTTAGCG GCAATTGCGA TGTGAACCGC ATGGAGCCCA AGAACCTGGC CATCATATTT 
GGGCCATCTA TAATCAGGAC GCCGAACGAT ACTCTGGAAA CGGCAGTCAA GGACATGAAG 
CATCAATGCC GCATCGTTGA GCTGCTGGTC ACACAGTACG ACTACTTCTT CGAAGGCGGC 
AGTCTGCCGG ATATAGCCGA AGTAAGCGGC GGTACAGCTG CCGTAAGCGC CGCTCAGCCG 
CAGCAGCAGA CGCAGGAGGA TCAGACGAGC ATGCTGCTGC ACAACCTGTC GAAGATCGAG 
CGGATCACAG AGCGCGAAAC CACGCGCACC TCGCGCTTTA TGCCGCAGCT CAGACGAAAG 
ACCCATGGCA AGCGCAGCGC CGTAAACTCG GACACGTACT CCGGCGAGAG CGTTCTGTCG 
TTGGACTACG CCAAGGCGTC CGCCTCTGCC TCGGCATCCG CAAGCAGCAC CGCCAGTACT 
TCGAGCAGCT CGACGCTTCA CAGCGGGGGC AAGGCATCCG CCTCATCCTC ATCCAGTTCC 
ACTACCAAGT TCAGCTCATC GTCATCGTCG GCGTGCTCAA CAACGGCGGT GGCTATTTCT 
TCCTCCATCT CGGCGACGTC CTCCAAATTG TTAAGCGCCA ATTTTAAGAA GCGTAGCACA 
GGAGGTTTCT TGGGCTCAAG ATCCTCGCAC CAGACGCGCA AGGCCAGCTT GGACGAAAAG 
GACTCAGGAC AGTCGTCTGG CAGCCTTGGT CACAGCCTGG GAGTAGGCAT AAATCTGGCC 
AAGGAACAAC AGCGCAGCAG CGTGGACATA TCCATTCTGC TAACGGACGA CGACTCTAGC 
AGCAGGCTAA GCGATACGGG TTCCATGTCG CTGACGACCA TTACGGATAC ACTGGACAGC 
AAGCTACGCA ACTTGCGCAG CGGCTCCGAA TCGAATGATG AGAATGGATC GCCCGAGTTT 
CCCAAGAACC GGCGGCACAC CTTGGGCCAG CCTCTGCACA TGCACTCCGA GAACATTCCG 
TATGCGGACG AGTCGCCCGA GCGGCTGTTC CATCAGTTTT GCAATCCCCT GGAGACAGCC 
CCCTTATCCC TGCATCTAAG CCGCTCCTGC ACGGCCACAA ATACAATTGG TGGCGATGAA 
AAGCCTTCGA AACAGTCGGG TGGAGTTGCA CCACTGCCCC CAAGTTTGCT TAAGGCCGCA 
CATAAGCTAC AGCACTCAGC CACGGTGCCC ATTCATCAGG GAAGCTCCAC TGCACCAACT 
AGCGGAGCAG CCACGCCAGT AGGCACGCCA ACCGGAAGCG GTGGGGCCGG AGCTATTCCC 
TCGCGCAGCT CATCGAGCAG TGTCACGACG CTAACCAAAA GCATACAACC CAGGTTCAGT 
AACTATCTAA GCTACGAAAG AGGCAATGCA GCCGCTGGAG GCGCCGAAAG GGTGGTGGGA 
TCCGCCGGAG GAGCTGCCTC CGACGACGAT TCCGAGGGCT CGACCACCAG TGATCCCAAG 
GAGAAGCTGC TTCTCGGTGA ACGACCGTCG CCTTCGTTTT TTATCGAGCG CTACAACAAA 
AAGAGACGCG ATCATCGGTT ATTTCGCAGC GCCAGTTTCA ATTGCCGGAA CTACTCCACC 
AGGCACATAA CGGCGCAGAC ATCCGGAGGA GTAGCGGCGG GAGTATCGGG CGGAGTAGCT 
GGAGCGGTGG CGGTGGGGGT GGCTTGCTGC CAGGCATTGA GCACCACTGG CTTGACCAAG 
GACGAGAAGA CTGATATGAA CCTGACCAAG AAGCGCCAGA TCCAGAACAA GCAGAATCGG 
TCCATTAAGC GGCGGCACAC AGTAGGCGGA CCTCACGACT ATTCCGCCAG CAATGGTGGC 
TGCTCGAATC ACTCACACGG TCACGACCTG GCAGCCATCA ACTACAATCA CCACAATCGC 
CATCACCAGC AACAGTTGTT GAAACTGGGC AGCGCTACAT CTGGCGGTGG AGGCAGTGGC 
GCAGCCGCGG CCGAGACGAC GACCACCACA ACGACCACCA CCACGGTGCT GCGGCGCCTC 
GGTACAGGTC AAGCTGGCGC CGAAGCTAGT GTGGCATTGC CCGCATCAAG TTCCGGATCG 
AGCAGCAACA ACAATAACTC GCCCCAAAGC GATGGCAGTA ATGGCAACGG CGGTGGAGTG 
GCGGGAGTTG CCTCCAATGC ACCACCAACT GGCGTAGGTG TCGGTGGCAA CAACAGCAGC 
GGAAGCAGCA GCAACAGCAG CAGCACCAGC ACAACTCCCG CTGGGAACGG CGATCAGGTC 
CTGGAAGTGG GCATACGTAT CAAGAACATT AGCCGGGGTC GCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RhoGAP19D ( NM_134552.6 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_608396.2 Drosophila melanogaster Rho GTPase activating protein at 19D (RhoGAP19D), transcript variant A, mRNA.

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CloneID	OFa03218
Clone ID Related Accession (Same CDS sequence)	NM_134552.6
Accession Version	NM_134552.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho GTPase activating protein at 19D, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_134552.5. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTACAAA ACTCGAACGG CGCTGCCGCA GCGCATTCGG CAGCCTCAGC GGTGGCGACT 
CCGGCAGCGG CGGCATCCGC AGCAGCAGCA GCTGCTGCAG CGGCGGTGGC TGCAGCGGCC 
AACAACGCCG CCCTGGCCGC CTCCTCCATC CAGCCCAAGC TCATCGTCAT CCGCCGGCGA 
CCCAACCAGG GATTCGGGTT CACGCTGCGC CACTTCATCG CCTATCCGCC GGAAGATGAC 
CAGGCATCAT CATCCGCATC GGGTTTGGTA TCGGGATCGG CGACAGCAGC AACGGCGGCA 
TCGGTATCGA CCAACTGGCC ACAGGAAGCC TCCAGTGCGG CGGGCAGCAA CAGCGGCAGC 
AGCAGCAGCG TCGGCGTCGC TGGGATCACC GGTTTGGAGC CAACGTCGCC GACGTCGCTG 
CCGCCGTACC AAGTGAAAGC GATGGAGACC ATTTTCATCA AAGAGGTGCA GGCGAACGGA 
CCGGCCCACT ACGCCAACCT GCAGACGGGC GACCGCGTGC TGATGGTCAA CAACCAGCCG 
ATCGCTGGCA TCGCCTACAG CACCATCGTC TCGATGATCA AGCAGACGCC GGCGGTCCTC 
ACGCTGCACG TGGTGCCCAA GGAGTGCGAT GTCCTGCAGA TGCACTATAC GAGCATTGCC 
CACACGCCGG AGTCGAATCG CCTGACCATG TCGACGCACT CGCCCTCGCT GCTGGCCAAC 
GGCTCCCACA AGACCACATT TCCAGCGGCG GCAGCAGTTG CAGCACCACC GCATCAGCAT 
CAGCAGCAGC AGCAACAGTT GATCTCATAT CCCGCTGTTG CAGCCGTGAC CACATCAACG 
CCGGCAGGAT CGCCGCAATC CCAATCCCAA TCCCAATCGC ACTCGCACCC CAACACCCAT 
CATGCACCAT CCCTGCACGG AGGCAGTCGA TCGGGCAGCA TCACCAGCAC GGCCAGCGGA 
GGCATCACCG TCCTCAGTCA GCCGTTCTAC CCACAGCAGC AGCAGCAACA GCAACAGCAG 
CAGCAACATC GTCATCCAGC GCTGACTGGC GGCAGCAGCA GCATCGATTT CGGGGACATG 
GCACATGGAC TGCGCCAGCA TCAGCAGCAG CAGCAACATC TGTACCAGCA GCAGCAACAG 
CATCACCAGT TTATGCGTGC TAATCAGCCG CCAAATTTAA CAGTATTATC GGCCAGCTCG 
GCAACGAACA CGCCCACACC CACCGCCCGC CAGCCCAAGT CCGCGACGAG CCACAATCAG 
GCGTTGTATG CAGTCGGAGC GGGTCCGAGC TCAGCAGCTG GATCTGGGTC GGGATCCCTT 
TCGGGGGGCA GTGAGAACAG CGACCTTATG ATCCGACTGC GCGAGTCCAT CAAGCAGAAG 
GAGGAGTTCC TTAAGTCGCC GGTGCCCGCC TCCATGAGCT CCGCCTCCTC TATCAACGGC 
AACCAGGCGC AGTCGTCATC GCCGGCACAG CAGCAACACC TGCAGCAGCA CCAGCAGCAC 
TTCTTTCCCT CGCACACGCC GCCAGGTGGT GTCCAAGTGG TGGTGCCTCC ACCGCGCAGT 
CGCCATCAAG TGATGCTCAA GCAGCAGCAG CAGCAACAGC AGCAGCACCA GCAACATCAT 
GTCCTTGGCT ACGACATGTA CCACTCCGGT GGTAAGCTGG TGCAGCGGTC TTCCACGGCG 
CCTGGCAGTG TGTGCCACCA ACACCAGCAG CAGCAGCAGC ATCACCTCTA TCACCAGCAG 
CAACATCAGC AGCTCCAGCA GACGCAACAT CGCCAGGTGA TGATCAACAA CAATGCCAAG 
TACGCCAAGG ATCTGGACTA TTTTGAGACG TTGCAGGAGA CAGGAGTCAC CAATAAGGTC 
TTTGAGCGCA AGCTGGTGTC ACACATGTCC AAGGGATTCG ATCCGTTTAA GCCGCTGTCG 
ATCAACACCA GTGCCCTGGA GTCGTCTGCC AGCACGTCGA GCACTTCGGC CACCACGACG 
GTGAAGAAGC AAAGTGTGCT GTATGAATCC CTGCAGCAGC ATCCATTGGC CAAGTACCAT 
CAGACGCAGC AGCAAACGAC AACGACGACG ACGGTGGATG GACAGACGAG TAGCCGGATG 
GAGCTGCACA AGGCGACCCT GGCGAATGCC ACCATCGATC CGGTGCCAGT TGGATCCAGC 
ACAGCAGCCA TTGTGGACAG TGCGTCGGGA AGCGGCAACG TTGTGCGGCG CTACAAGCCA 
TTATCCTCCA GCTCCTTTGA CGAAGGCAAA CCAATGCGTA GGATATCCTA CCTGCGGGCA 
ACCAACAATG AGACCGACTT CAATGCGGAG GCTGCCGCTG GCGGAGATGC CGCGGCAACA 
GGGGCGTCAG GACCAGCATC TGCATCAGCA GCTTCATCTG CACCTGGACC CACGCCCTCA 
TCTGCTCCTG CACCAGCTCC AGCACCAGCA CCCCTGCCCA TTTCGATCCC CATTCCGGTG 
GCTGCTGTCG TCGAGCCGCA AAAGACGAAG TCCCTGGTGG AGGAGCATCC GGATCCCACC 
TACGATGTGA TCGGAGGCAC AGGTGGTGGC CACGAGTTCA TCGAGACGCC GAACGGCCTG 
GAGGATGTGC GTCTGTCGGC CCACGGGAGT CGGAGGGCCT CCATGAGCAG TGACATGCGA 
AGCAGCATCC ACATTGATGC CGAGTTAAAT GGCAAGTATG TGCCAAACGA ACTGGAGGCC 
TTTGAGACGG AAATCGAGAT CAAGTCCTCT TGCATCAACG GAAAACGCAT GTCCGAGTAC 
CGATCATGGC GTCAGGTGAA GCTGGAGATC AAGGGAGATC TGCTGCGTAT CTACTCAGGC 
CGACATGCCA AATCGGAGCA CAATGTGATA GAACTTGATA TTAGAAACTT TAAGTTCTTC 
GACGAGTCGA TGGATAAGAA GAAATACTTG ATCCGTATGC AGTCGAAGCC ATCGCCCAGT 
GGAGCCCATT TGGCCTCCTT GGGCAACGAG TTAAACCTGG ACGAGGGTCA CGACAAGCTG 
CTAACCTCGT CGGGCAGCAG CAGTGCGAAT GAGCCCGCTG CCAACGCAGC ATCGGCCCTG 
ACCGCATCCA CCTCCTCGTC CACCTGCAGC AGCGGTCCTT ACACGGAGAT CCTATTCAAG 
ACGAAGAGCA GCAACGAGAT GAAGCGTCTG TTTGGACTTC TGCAGTGGAA GGATAGCCTC 
AACTACGATG ATAATGAGCA CCAGCCACAG GGCAAGCAGT TGGCAGAACC ACAGAAAACA 
GTGGCCAGCG CCAGTTATCT GGACGACGTC CAAGGTGGAG CGAGTGCAGC CGACAGCTCA 
CCCTTGAGCT CCCATTCCGG TGGCATTGCA GACGCTAGTG GCAGTCATCA TGTTTCTGCG 
CTACCAGCAG CTGCGGCTAT TGTCGCCGCC ACCAGCGACT CCATTTCGCC GGTGATGAAG 
GCAAGGAAGT CCTCTTCGCA CAAGCATATA CCTGATAAGG ATTTGGGATC GCCCAAATCG 
AAGAACTGGA AGGATTTGCT CTTCCGCCGT GGCGGCAGTG GCAGCGGAGG AGTGTCACAC 
CACGACTTGG CTTCGCCATC CGCCTGTGCA GCCAAGCAGA TTGGATCTAT TGGCGTTCCA 
CTGCGCAGCT GTCCCATGTC GAAGGTCAAT GCATATGTGC CGCATCTGGT AGAGGTGTGC 
ACGAACATTG TGGAGACAAA GGGTCTGGGA GTGGTGGGCA TCTATCGCAT ACCCGGCAAC 
AAGGCGGCCA TATCAGAGCT GTCCGAACTT GTTAACACCA AGGACTTTCA GTTCGAGAGC 
TGTGCCAGCG ATGACCGCTG GGAGGATGTG AATGTGGTGA GCAGCCTGCT CAAGTTGTTT 
ATACGCAGCT TGCCCGATGC CTTAATGCCG GCAAGTTACT ATATCAACTT TATTGAGGCG 
GACAAGAAGT TTGGCCTGGA GAGAATTGTA CTGTTGCGCG AGATTGTGGA ATCGTTACCA 
CGGCATCCCT ACGAAACGAT GAAGCACTTG ATTCGGCATC TGTGCCGAGT TAGCGGCAAT 
TGCGATGTGA ACCGCATGGA GCCCAAGAAC CTGGCCATCA TATTTGGGCC ATCTATAATC 
AGGACGCCGA ACGATACTCT GGAAACGGCA GTCAAGGACA TGAAGCATCA ATGCCGCATC 
GTTGAGCTGC TGGTCACACA GTACGACTAC TTCTTCGAAG GCGGCAGTCT GCCGGATATA 
GCCGAAGTAA GCGGCGGTAC AGCTGCCGTA AGCGCCGCTC AGCCGCAGCA GCAGACGCAG 
GAGGATCAGA CGAGCATGCT GCTGCACAAC CTGTCGAAGA TCGAGCGGAT CACAGAGCGC 
GAAACCACGC GCACCTCGCG CTTTATGCCG CAGCTCAGAC GAAAGACCCA TGGCAAGCGC 
AGCGCCGTAA ACTCGGACAC GTACTCCGGC GAGAGCGTTC TGGTAAGCGG GAGCTCCTCC 
AACACCACCA CCACCACCAC CACCACCACC CACACCACAA GTAGCACAAG TAGTAGCAAC 
ACCATCACCA CAACCATCAG CACCACCACC ATCCAGCAGC GACGGGCGCA GCGCAAGGCT 
GTGCAGAGCA TTTGCGACCA GGCTCTAATC CTGCTCCTGC CCACACCCGT TTCTTCCGTC 
TCTCTTCCCT TTCAGTCGTT GGACTACGCC AAGGCGTCCG CCTCTGCCTC GGCATCCGCA 
AGCAGCACCG CCAGTACTTC GAGCAGCTCG ACGCTTCACA GCGGGGGCAA GGCATCCGCC 
TCATCCTCAT CCAGTTCCAC TACCAAGTTC AGCTCATCGT CATCGTCGGC GTGCTCAACA 
ACGGCGGTGG CTATTTCTTC CTCCATCTCG GCGACGTCCT CCAAATTGTT AAGCGCCAAT 
TTTAAGAAGC GTAGCACAGG AGGTTTCTTG GGCTCAAGAT CCTCGCACCA GACGCGCAAG 
GCCAGCTTGG ACGAAAAGGA CTCAGGACAG TCGTCTGGCA GCCTTGGTCA CAGCCTGGGA 
GTAGGCATAA ATCTGGCCAA GGAACAACAG CGCAGCAGCG TGGACATATC CATTCTGCTA 
ACGGACGACG ACTCTAGCAG CAGGCTAAGC GATACGGGTT CCATGTCGCT GACGACCATT 
ACGGATACAC TGGACAGCAA GCTACGCAAC TTGCGCAGCG GCTCCGAATC GAATGATGAG 
AATGGATCGC CCGAGTTTCC CAAGAACCGG CGGCACACCT TGGGCCAGCC TCTGCACATG 
CACTCCGAGA ACATTCCGTA TGCGGACGAG TCGCCCGAGC GGCTGTTCCA TCAGTTTTGC 
AATCCCCTGG AGACAGCCCC CTTATCCCTG CATCTAAGCC GCTCCTGCAC GGCCACAAAT 
ACAATTGGTG GCGATGAAAA GCCTTCGAAA CAGTCGGGTG GAGTTGCACC ACTGCCCCCA 
AGTTTGCTTA AGGCCGCACA TAAGCTACAG CACTCAGCCA CGGTGCCCAT TCATCAGGGA 
AGCTCCACTG CACCAACTAG CGGAGCAGCC ACGCCAGTAG GCACGCCAAC CGGAAGCGGT 
GGGGCCGGAG CTATTCCCTC GCGCAGCTCA TCGAGCAGTG TCACGACGCT AACCAAAAGC 
ATACAACCCA GGTTCAGTAA CTATCTAAGC TACGAAAGAG GCAATGCAGC CGCTGGAGGC 
GCCGAAAGGG TGGTGGGATC CGCCGGAGGA GCTGCCTCCG ACGACGATTC CGAGGGCTCG 
ACCACCAGTG ATCCCAAGGA GAAGCTGCTT CTCGGTGAAC GACCGTCGCC TTCGTTTTTT 
ATCGAGCGCT ACAACAAAAA GAGACGCGAT CATCGGTTAT TTCGCAGCGC CAGTTTCAAT 
TGCCGGAACT ACTCCACCAG GCACATAACG GCGCAGACAT CCGGAGGAGT AGCGGCGGGA 
GTATCGGGCG GAGTAGCTGG AGCGGTGGCG GTGGGGGTGG CTTGCTGCCA GGCATTGAGC 
ACCACTGGCT TGACCAAGGA CGAGAAGACT GATATGAACC TGACCAAGAA GCGCCAGATC 
CAGAACAAGC AGAATCGGTC CATTAAGCGG CGGCACACAG TAGGCGGACC TCACGACTAT 
TCCGCCAGCA ATGGTGGCTG CTCGAATCAC TCACACGGTC ACGACCTGGC AGCCATCAAC 
TACAATCACC ACAATCGCCA TCACCAGCAA CAGTTGTTGA AACTGGGCAG CGCTACATCT 
GGCGGTGGAG GCAGTGGCGC AGCCGCGGCC GAGACGACGA CCACCACAAC GACCACCACC 
ACGGTGCTGC GGCGCCTCGG TACAGGTCAA GCTGGCGCCG AAGCTAGTGT GGCATTGCCC 
GCATCAAGTT CCGGATCGAG CAGCAACAAC AATAACTCGC CCCAAAGCGA TGGCAGTAAT 
GGCAACGGCG GTGGAGTGGC GGGAGTTGCC TCCAATGCAC CACCAACTGG CGTAGGTGTC 
GGTGGCAACA ACAGCAGCGG AAGCAGCAGC AACAGCAGCA GCACCAGCAC AACTCCCGCT 
GGGAACGGCG ATCAGGTCCT GGAAGTGGGC ATACGTATCA AGAACATTAG CCGGGGTCGC 
TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_608396.2
CDS	803..7348
Translation	MLQNSNGAAAAHSAASAVATPAAAASAAAAAAAAAVAAAANNAALAASSIQPKLIVIRRRPNQGFGFTLRHFIAYPPEDDQASSSASGLVSGSATAATAASVSTNWPQEASSAAGSNSGSSSSVGVAGITGLEPTSPTSLPPYQVKAMETIFIKEVQANGPAHYANLQTGDRVLMVNNQPIAGIAYSTIVSMIKQTPAVLTLHVVPKECDVLQMHYTSIAHTPESNRLTMSTHSPSLLANGSHKTTFPAAAAVAAPPHQHQQQQQQLISYPAVAAVTTSTPAGSPQSQSQSQSHSHPNTHHAPSLHGGSRSGSITSTASGGITVLSQPFYPQQQQQQQQQQQHRHPALTGGSSSIDFGDMAHGLRQHQQQQQHLYQQQQQHHQFMRANQPPNLTVLSASSATNTPTPTARQPKSATSHNQALYAVGAGPSSAAGSGSGSLSGGSENSDLMIRLRESIKQKEEFLKSPVPASMSSASSINGNQAQSSSPAQQQHLQQHQQHFFPSHTPPGGVQVVVPPPRSRHQVMLKQQQQQQQQHQQHHVLGYDMYHSGGKLVQRSSTAPGSVCHQHQQQQQHHLYHQQQHQQLQQTQHRQVMINNNAKYAKDLDYFETLQETGVTNKVFERKLVSHMSKGFDPFKPLSINTSALESSASTSSTSATTTVKKQSVLYESLQQHPLAKYHQTQQQTTTTTTVDGQTSSRMELHKATLANATIDPVPVGSSTAAIVDSASGSGNVVRRYKPLSSSSFDEGKPMRRISYLRATNNETDFNAEAAAGGDAAATGASGPASASAASSAPGPTPSSAPAPAPAPAPLPISIPIPVAAVVEPQKTKSLVEEHPDPTYDVIGGTGGGHEFIETPNGLEDVRLSAHGSRRASMSSDMRSSIHIDAELNGKYVPNELEAFETEIEIKSSCINGKRMSEYRSWRQVKLEIKGDLLRIYSGRHAKSEHNVIELDIRNFKFFDESMDKKKYLIRMQSKPSPSGAHLASLGNELNLDEGHDKLLTSSGSSSANEPAANAASALTASTSSSTCSSGPYTEILFKTKSSNEMKRLFGLLQWKDSLNYDDNEHQPQGKQLAEPQKTVASASYLDDVQGGASAADSSPLSSHSGGIADASGSHHVSALPAAAAIVAATSDSISPVMKARKSSSHKHIPDKDLGSPKSKNWKDLLFRRGGSGSGGVSHHDLASPSACAAKQIGSIGVPLRSCPMSKVNAYVPHLVEVCTNIVETKGLGVVGIYRIPGNKAAISELSELVNTKDFQFESCASDDRWEDVNVVSSLLKLFIRSLPDALMPASYYINFIEADKKFGLERIVLLREIVESLPRHPYETMKHLIRHLCRVSGNCDVNRMEPKNLAIIFGPSIIRTPNDTLETAVKDMKHQCRIVELLVTQYDYFFEGGSLPDIAEVSGGTAAVSAAQPQQQTQEDQTSMLLHNLSKIERITERETTRTSRFMPQLRRKTHGKRSAVNSDTYSGESVLVSGSSSNTTTTTTTTTHTTSSTSSSNTITTTISTTTIQQRRAQRKAVQSICDQALILLLPTPVSSVSLPFQSLDYAKASASASASASSTASTSSSSTLHSGGKASASSSSSSTTKFSSSSSSACSTTAVAISSSISATSSKLLSANFKKRSTGGFLGSRSSHQTRKASLDEKDSGQSSGSLGHSLGVGINLAKEQQRSSVDISILLTDDDSSSRLSDTGSMSLTTITDTLDSKLRNLRSGSESNDENGSPEFPKNRRHTLGQPLHMHSENIPYADESPERLFHQFCNPLETAPLSLHLSRSCTATNTIGGDEKPSKQSGGVAPLPPSLLKAAHKLQHSATVPIHQGSSTAPTSGAATPVGTPTGSGGAGAIPSRSSSSSVTTLTKSIQPRFSNYLSYERGNAAAGGAERVVGSAGGAASDDDSEGSTTSDPKEKLLLGERPSPSFFIERYNKKRRDHRLFRSASFNCRNYSTRHITAQTSGGVAAGVSGGVAGAVAVGVACCQALSTTGLTKDEKTDMNLTKKRQIQNKQNRSIKRRHTVGGPHDYSASNGGCSNHSHGHDLAAINYNHHNRHHQQQLLKLGSATSGGGGSGAAAAETTTTTTTTTTVLRRLGTGQAGAEASVALPASSSGSSSNNNNSPQSDGSNGNGGGVAGVASNAPPTGVGVGGNNSSGSSSNSSSTSTTPAGNGDQVLEVGIRIKNISRGRF

Target ORF information:

RefSeq Version	NM_134552.6
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho GTPase activating protein at 19D (RhoGAP19D), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134552.6

ORF Insert Sequence:

ATGTTACAAA ACTCGAACGG CGCTGCCGCA GCGCATTCGG CAGCCTCAGC GGTGGCGACT 
CCGGCAGCGG CGGCATCCGC AGCAGCAGCA GCTGCTGCAG CGGCGGTGGC TGCAGCGGCC 
AACAACGCCG CCCTGGCCGC CTCCTCCATC CAGCCCAAGC TCATCGTCAT CCGCCGGCGA 
CCCAACCAGG GATTCGGGTT CACGCTGCGC CACTTCATCG CCTATCCGCC GGAAGATGAC 
CAGGCATCAT CATCCGCATC GGGTTTGGTA TCGGGATCGG CGACAGCAGC AACGGCGGCA 
TCGGTATCGA CCAACTGGCC ACAGGAAGCC TCCAGTGCGG CGGGCAGCAA CAGCGGCAGC 
AGCAGCAGCG TCGGCGTCGC TGGGATCACC GGTTTGGAGC CAACGTCGCC GACGTCGCTG 
CCGCCGTACC AAGTGAAAGC GATGGAGACC ATTTTCATCA AAGAGGTGCA GGCGAACGGA 
CCGGCCCACT ACGCCAACCT GCAGACGGGC GACCGCGTGC TGATGGTCAA CAACCAGCCG 
ATCGCTGGCA TCGCCTACAG CACCATCGTC TCGATGATCA AGCAGACGCC GGCGGTCCTC 
ACGCTGCACG TGGTGCCCAA GGAGTGCGAT GTCCTGCAGA TGCACTATAC GAGCATTGCC 
CACACGCCGG AGTCGAATCG CCTGACCATG TCGACGCACT CGCCCTCGCT GCTGGCCAAC 
GGCTCCCACA AGACCACATT TCCAGCGGCG GCAGCAGTTG CAGCACCACC GCATCAGCAT 
CAGCAGCAGC AGCAACAGTT GATCTCATAT CCCGCTGTTG CAGCCGTGAC CACATCAACG 
CCGGCAGGAT CGCCGCAATC CCAATCCCAA TCCCAATCGC ACTCGCACCC CAACACCCAT 
CATGCACCAT CCCTGCACGG AGGCAGTCGA TCGGGCAGCA TCACCAGCAC GGCCAGCGGA 
GGCATCACCG TCCTCAGTCA GCCGTTCTAC CCACAGCAGC AGCAGCAACA GCAACAGCAG 
CAGCAACATC GTCATCCAGC GCTGACTGGC GGCAGCAGCA GCATCGATTT CGGGGACATG 
GCACATGGAC TGCGCCAGCA TCAGCAGCAG CAGCAACATC TGTACCAGCA GCAGCAACAG 
CATCACCAGT TTATGCGTGC TAATCAGCCG CCAAATTTAA CAGTATTATC GGCCAGCTCG 
GCAACGAACA CGCCCACACC CACCGCCCGC CAGCCCAAGT CCGCGACGAG CCACAATCAG 
GCGTTGTATG CAGTCGGAGC GGGTCCGAGC TCAGCAGCTG GATCTGGGTC GGGATCCCTT 
TCGGGGGGCA GTGAGAACAG CGACCTTATG ATCCGACTGC GCGAGTCCAT CAAGCAGAAG 
GAGGAGTTCC TTAAGTCGCC GGTGCCCGCC TCCATGAGCT CCGCCTCCTC TATCAACGGC 
AACCAGGCGC AGTCGTCATC GCCGGCACAG CAGCAACACC TGCAGCAGCA CCAGCAGCAC 
TTCTTTCCCT CGCACACGCC GCCAGGTGGT GTCCAAGTGG TGGTGCCTCC ACCGCGCAGT 
CGCCATCAAG TGATGCTCAA GCAGCAGCAG CAGCAACAGC AGCAGCACCA GCAACATCAT 
GTCCTTGGCT ACGACATGTA CCACTCCGGT GGTAAGCTGG TGCAGCGGTC TTCCACGGCG 
CCTGGCAGTG TGTGCCACCA ACACCAGCAG CAGCAGCAGC ATCACCTCTA TCACCAGCAG 
CAACATCAGC AGCTCCAGCA GACGCAACAT CGCCAGGTGA TGATCAACAA CAATGCCAAG 
TACGCCAAGG ATCTGGACTA TTTTGAGACG TTGCAGGAGA CAGGAGTCAC CAATAAGGTC 
TTTGAGCGCA AGCTGGTGTC ACACATGTCC AAGGGATTCG ATCCGTTTAA GCCGCTGTCG 
ATCAACACCA GTGCCCTGGA GTCGTCTGCC AGCACGTCGA GCACTTCGGC CACCACGACG 
GTGAAGAAGC AAAGTGTGCT GTATGAATCC CTGCAGCAGC ATCCATTGGC CAAGTACCAT 
CAGACGCAGC AGCAAACGAC AACGACGACG ACGGTGGATG GACAGACGAG TAGCCGGATG 
GAGCTGCACA AGGCGACCCT GGCGAATGCC ACCATCGATC CGGTGCCAGT TGGATCCAGC 
ACAGCAGCCA TTGTGGACAG TGCGTCGGGA AGCGGCAACG TTGTGCGGCG CTACAAGCCA 
TTATCCTCCA GCTCCTTTGA CGAAGGCAAA CCAATGCGTA GGATATCCTA CCTGCGGGCA 
ACCAACAATG AGACCGACTT CAATGCGGAG GCTGCCGCTG GCGGAGATGC CGCGGCAACA 
GGGGCGTCAG GACCAGCATC TGCATCAGCA GCTTCATCTG CACCTGGACC CACGCCCTCA 
TCTGCTCCTG CACCAGCTCC AGCACCAGCA CCCCTGCCCA TTTCGATCCC CATTCCGGTG 
GCTGCTGTCG TCGAGCCGCA AAAGACGAAG TCCCTGGTGG AGGAGCATCC GGATCCCACC 
TACGATGTGA TCGGAGGCAC AGGTGGTGGC CACGAGTTCA TCGAGACGCC GAACGGCCTG 
GAGGATGTGC GTCTGTCGGC CCACGGGAGT CGGAGGGCCT CCATGAGCAG TGACATGCGA 
AGCAGCATCC ACATTGATGC CGAGTTAAAT GGCAAGTATG TGCCAAACGA ACTGGAGGCC 
TTTGAGACGG AAATCGAGAT CAAGTCCTCT TGCATCAACG GAAAACGCAT GTCCGAGTAC 
CGATCATGGC GTCAGGTGAA GCTGGAGATC AAGGGAGATC TGCTGCGTAT CTACTCAGGC 
CGACATGCCA AATCGGAGCA CAATGTGATA GAACTTGATA TTAGAAACTT TAAGTTCTTC 
GACGAGTCGA TGGATAAGAA GAAATACTTG ATCCGTATGC AGTCGAAGCC ATCGCCCAGT 
GGAGCCCATT TGGCCTCCTT GGGCAACGAG TTAAACCTGG ACGAGGGTCA CGACAAGCTG 
CTAACCTCGT CGGGCAGCAG CAGTGCGAAT GAGCCCGCTG CCAACGCAGC ATCGGCCCTG 
ACCGCATCCA CCTCCTCGTC CACCTGCAGC AGCGGTCCTT ACACGGAGAT CCTATTCAAG 
ACGAAGAGCA GCAACGAGAT GAAGCGTCTG TTTGGACTTC TGCAGTGGAA GGATAGCCTC 
AACTACGATG ATAATGAGCA CCAGCCACAG GGCAAGCAGT TGGCAGAACC ACAGAAAACA 
GTGGCCAGCG CCAGTTATCT GGACGACGTC CAAGGTGGAG CGAGTGCAGC CGACAGCTCA 
CCCTTGAGCT CCCATTCCGG TGGCATTGCA GACGCTAGTG GCAGTCATCA TGTTTCTGCG 
CTACCAGCAG CTGCGGCTAT TGTCGCCGCC ACCAGCGACT CCATTTCGCC GGTGATGAAG 
GCAAGGAAGT CCTCTTCGCA CAAGCATATA CCTGATAAGG ATTTGGGATC GCCCAAATCG 
AAGAACTGGA AGGATTTGCT CTTCCGCCGT GGCGGCAGTG GCAGCGGAGG AGTGTCACAC 
CACGACTTGG CTTCGCCATC CGCCTGTGCA GCCAAGCAGA TTGGATCTAT TGGCGTTCCA 
CTGCGCAGCT GTCCCATGTC GAAGGTCAAT GCATATGTGC CGCATCTGGT AGAGGTGTGC 
ACGAACATTG TGGAGACAAA GGGTCTGGGA GTGGTGGGCA TCTATCGCAT ACCCGGCAAC 
AAGGCGGCCA TATCAGAGCT GTCCGAACTT GTTAACACCA AGGACTTTCA GTTCGAGAGC 
TGTGCCAGCG ATGACCGCTG GGAGGATGTG AATGTGGTGA GCAGCCTGCT CAAGTTGTTT 
ATACGCAGCT TGCCCGATGC CTTAATGCCG GCAAGTTACT ATATCAACTT TATTGAGGCG 
GACAAGAAGT TTGGCCTGGA GAGAATTGTA CTGTTGCGCG AGATTGTGGA ATCGTTACCA 
CGGCATCCCT ACGAAACGAT GAAGCACTTG ATTCGGCATC TGTGCCGAGT TAGCGGCAAT 
TGCGATGTGA ACCGCATGGA GCCCAAGAAC CTGGCCATCA TATTTGGGCC ATCTATAATC 
AGGACGCCGA ACGATACTCT GGAAACGGCA GTCAAGGACA TGAAGCATCA ATGCCGCATC 
GTTGAGCTGC TGGTCACACA GTACGACTAC TTCTTCGAAG GCGGCAGTCT GCCGGATATA 
GCCGAAGTAA GCGGCGGTAC AGCTGCCGTA AGCGCCGCTC AGCCGCAGCA GCAGACGCAG 
GAGGATCAGA CGAGCATGCT GCTGCACAAC CTGTCGAAGA TCGAGCGGAT CACAGAGCGC 
GAAACCACGC GCACCTCGCG CTTTATGCCG CAGCTCAGAC GAAAGACCCA TGGCAAGCGC 
AGCGCCGTAA ACTCGGACAC GTACTCCGGC GAGAGCGTTC TGGTAAGCGG GAGCTCCTCC 
AACACCACCA CCACCACCAC CACCACCACC CACACCACAA GTAGCACAAG TAGTAGCAAC 
ACCATCACCA CAACCATCAG CACCACCACC ATCCAGCAGC GACGGGCGCA GCGCAAGGCT 
GTGCAGAGCA TTTGCGACCA GGCTCTAATC CTGCTCCTGC CCACACCCGT TTCTTCCGTC 
TCTCTTCCCT TTCAGTCGTT GGACTACGCC AAGGCGTCCG CCTCTGCCTC GGCATCCGCA 
AGCAGCACCG CCAGTACTTC GAGCAGCTCG ACGCTTCACA GCGGGGGCAA GGCATCCGCC 
TCATCCTCAT CCAGTTCCAC TACCAAGTTC AGCTCATCGT CATCGTCGGC GTGCTCAACA 
ACGGCGGTGG CTATTTCTTC CTCCATCTCG GCGACGTCCT CCAAATTGTT AAGCGCCAAT 
TTTAAGAAGC GTAGCACAGG AGGTTTCTTG GGCTCAAGAT CCTCGCACCA GACGCGCAAG 
GCCAGCTTGG ACGAAAAGGA CTCAGGACAG TCGTCTGGCA GCCTTGGTCA CAGCCTGGGA 
GTAGGCATAA ATCTGGCCAA GGAACAACAG CGCAGCAGCG TGGACATATC CATTCTGCTA 
ACGGACGACG ACTCTAGCAG CAGGCTAAGC GATACGGGTT CCATGTCGCT GACGACCATT 
ACGGATACAC TGGACAGCAA GCTACGCAAC TTGCGCAGCG GCTCCGAATC GAATGATGAG 
AATGGATCGC CCGAGTTTCC CAAGAACCGG CGGCACACCT TGGGCCAGCC TCTGCACATG 
CACTCCGAGA ACATTCCGTA TGCGGACGAG TCGCCCGAGC GGCTGTTCCA TCAGTTTTGC 
AATCCCCTGG AGACAGCCCC CTTATCCCTG CATCTAAGCC GCTCCTGCAC GGCCACAAAT 
ACAATTGGTG GCGATGAAAA GCCTTCGAAA CAGTCGGGTG GAGTTGCACC ACTGCCCCCA 
AGTTTGCTTA AGGCCGCACA TAAGCTACAG CACTCAGCCA CGGTGCCCAT TCATCAGGGA 
AGCTCCACTG CACCAACTAG CGGAGCAGCC ACGCCAGTAG GCACGCCAAC CGGAAGCGGT 
GGGGCCGGAG CTATTCCCTC GCGCAGCTCA TCGAGCAGTG TCACGACGCT AACCAAAAGC 
ATACAACCCA GGTTCAGTAA CTATCTAAGC TACGAAAGAG GCAATGCAGC CGCTGGAGGC 
GCCGAAAGGG TGGTGGGATC CGCCGGAGGA GCTGCCTCCG ACGACGATTC CGAGGGCTCG 
ACCACCAGTG ATCCCAAGGA GAAGCTGCTT CTCGGTGAAC GACCGTCGCC TTCGTTTTTT 
ATCGAGCGCT ACAACAAAAA GAGACGCGAT CATCGGTTAT TTCGCAGCGC CAGTTTCAAT 
TGCCGGAACT ACTCCACCAG GCACATAACG GCGCAGACAT CCGGAGGAGT AGCGGCGGGA 
GTATCGGGCG GAGTAGCTGG AGCGGTGGCG GTGGGGGTGG CTTGCTGCCA GGCATTGAGC 
ACCACTGGCT TGACCAAGGA CGAGAAGACT GATATGAACC TGACCAAGAA GCGCCAGATC 
CAGAACAAGC AGAATCGGTC CATTAAGCGG CGGCACACAG TAGGCGGACC TCACGACTAT 
TCCGCCAGCA ATGGTGGCTG CTCGAATCAC TCACACGGTC ACGACCTGGC AGCCATCAAC 
TACAATCACC ACAATCGCCA TCACCAGCAA CAGTTGTTGA AACTGGGCAG CGCTACATCT 
GGCGGTGGAG GCAGTGGCGC AGCCGCGGCC GAGACGACGA CCACCACAAC GACCACCACC 
ACGGTGCTGC GGCGCCTCGG TACAGGTCAA GCTGGCGCCG AAGCTAGTGT GGCATTGCCC 
GCATCAAGTT CCGGATCGAG CAGCAACAAC AATAACTCGC CCCAAAGCGA TGGCAGTAAT 
GGCAACGGCG GTGGAGTGGC GGGAGTTGCC TCCAATGCAC CACCAACTGG CGTAGGTGTC 
GGTGGCAACA ACAGCAGCGG AAGCAGCAGC AACAGCAGCA GCACCAGCAC AACTCCCGCT 
GGGAACGGCG ATCAGGTCCT GGAAGTGGGC ATACGTATCA AGAACATTAG CCGGGGTCGC 
TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RhoGAP19D ( NM_001298567.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285496.1 Drosophila melanogaster Rho GTPase activating protein at 19D (RhoGAP19D), transcript variant D, mRNA.

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CloneID	OFa21558
Clone ID Related Accession (Same CDS sequence)	NM_001298567.1
Accession Version	NM_001298567.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6621bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho GTPase activating protein at 19D, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTACAAA ACTCGAACGG CGCTGCCGCA GCGCATTCGG CAGCCTCAGC GGTGGCGACT 
CCGGCAGCGG CGGCATCCGC AGCAGCAGCA GCTGCTGCAG CGGCGGTGGC TGCAGCGGCC 
AACAACGCCG CCCTGGCCGC CTCCTCCATC CAGCCCAAGC TCATCGTCAT CCGCCGGCGA 
CCCAACCAGG GATTCGGGTT CACGCTGCGC CACTTCATCG CCTATCCGCC GGAAGATGAC 
CAGGCATCAT CATCCGCATC GGGTTTGGTA TCGGGATCGG CGACAGCAGC AACGGCGGCA 
TCGGTATCGA CCAACTGGCC ACAGGAAGCC TCCAGTGCGG CGGGCAGCAA CAGCGGCAGC 
AGCAGCAGCG TCGGCGTCGC TGGGATCACC GGTTTGGAGC CAACGTCGCC GACGTCGCTG 
CCGCCGTACC AAGTGAAAGC GATGGAGACC ATTTTCATCA AAGAGGTGCA GGCGAACGGA 
CCGGCCCACT ACGCCAACCT GCAGACGGGC GACCGCGTGC TGATGGTCAA CAACCAGCCG 
ATCGCTGGCA TCGCCTACAG CACCATCGTC TCGATGATCA AGCAGACGCC GGCGGTCCTC 
ACGCTGCACG TGGTGCCCAA GGAGTGCGAT GTCCTGCAGA TGCACTATAC GAGCATTGCC 
CACACGCCGG AGTCGAATCG CCTGACCATG TCGACGCACT CGCCCTCGCT GCTGGCCAAC 
GGCTCCCACA AGACCACATT TCCAGCGGCG GCAGCAGTTG CAGCACCACC GCATCAGCAT 
CAGCAGCAGC AGCAACAGTT GATCTCATAT CCCGCTGTTG CAGCCGTGAC CACATCAACG 
CCGGCAGGAT CGCCGCAATC CCAATCCCAA TCCCAATCGC ACTCGCACCC CAACACCCAT 
CATGCACCAT CCCTGCACGG AGGCAGTCGA TCGGGCAGCA TCACCAGCAC GGCCAGCGGA 
GGCATCACCG TCCTCAGTCA GCCGTTCTAC CCACAGCAGC AGCAGCAACA GCAACAGCAG 
CAGCAACATC GTCATCCAGC GCTGACTGGC GGCAGCAGCA GCATCGATTT CGGGGACATG 
GCACATGGAC TGCGCCAGCA TCAGCAGCAG CAGCAACATC TGTACCAGCA GCAGCAACAG 
CATCACCAGT TTATGCGTGC TAATCAGCCG CCAAATTTAA CAGTATTATC GGCCAGCTCG 
GCAACGAACA CGCCCACACC CACCGCCCGC CAGCCCAAGT CCGCGACGAG CCACAATCAG 
GCGTTGTATG CAGTCGGAGC GGGTCCGAGC TCAGCAGCTG GATCTGGGTC GGGATCCCTT 
TCGGGGGGCA GTGAGAACAG CGACCTTATG ATCCGACTGC GCGAGTCCAT CAAGCAGAAG 
GAGGAGTTCC TTAAGTCGCC GGTGCCCGCC TCCATGAGCT CCGCCTCCTC TATCAACGGC 
AACCAGGCGC AGTCGTCATC GCCGGCACAG CAGCAACACC TGCAGCAGCA CCAGCAGCAC 
TTCTTTCCCT CGCACACGCC GCCAGGTGGT GTCCAAGTGG TGGTGCCTCC ACCGCGCAGT 
CGCCATCAAG TGATGCTCAA GCAGCAGCAG CAGCAACAGC AGCAGCACCA GCAACATCAT 
GTCCTTGGCT ACGACATGTA CCACTCCGGT GGTAAGCTGG TGCAGCGGTC TTCCACGGCG 
CCTGGCAGTG TGTGCCACCA ACACCAGCAG CAGCAGCAGC ATCACCTCTA TCACCAGCAG 
CAACATCAGC AGCTCCAGCA GACGCAACAT CGCCAGGTGA TGATCAACAA CAATGCCAAG 
TACGCCAAGG ATCTGGACTA TTTTGAGACG TTGCAGGAGA CAGGAGTCAC CAATAAGGTC 
TTTGAGCGCA AGCTGGTGTC ACACATGTCC AAGGGATTCG ATCCGTTTAA GCCGCTGTCG 
ATCAACACCA GTGCCCTGGA GTCGTCTGCC AGCACGTCGA GCACTTCGGC CACCACGACG 
GTGAAGAAGC AAAGTGTGCT GTATGAATCC CTGCAGCAGC ATCCATTGGC CAAGTACCAT 
CAGACGCAGC AGCAAACGAC AACGACGACG ACGGTGGATG GACAGACGAG TAGCCGGATG 
GAGCTGCACA AGGCGACCCT GGCGAATGCC ACCATCGATC CGGTGCCAGT TGGATCCAGC 
AAGTTTGTGA CGCTGCCGGA TAAGCCGGAT AAGGGTTCCT CCGTTGCCAC CTTGGCGGAC 
ATCGCCGAGA GCAGTGCAGC AGCCATTGTG GACAGTGCGT CGGGAAGCGG CAACGTTGTG 
CGGCGCTACA AGCCATTATC CTCCAGCTCC TTTGACGAAG GCAAACCAAT GCGTAGGATA 
TCCTACCTGC GGGCAACCAA CAATGAGACC GACTTCAATG CGGAGGCTGC CGCTGGCGGA 
GATGCCGCGG CAACAGGGGC GTCAGGACCA GCATCTGCAT CAGCAGCTTC ATCTGCACCT 
GGACCCACGC CCTCATCTGC TCCTGCACCA GCTCCAGCAC CAGCACCCCT GCCCATTTCG 
ATCCCCATTC CGGTGGCTGC TGTCGTCGAG CCGCAAAAGA CGAAGTCCCT GGTGGAGGAG 
CATCCGGATC CCACCTACGA TGTGATCGGA GGCACAGGTG GTGGCCACGA GTTCATCGAG 
ACGCCGAACG GCCTGGAGGA TGTGCGTCTG TCGGCCCACG GGAGTCGGAG GGCCTCCATG 
AGCAGTGACA TGCGAAGCAG CATCCACATT GATGCCGAGT TAAATGGCAA GTATGTGCCA 
AACGAACTGG AGGCCTTTGA GACGGAAATC GAGATCAAGT CCTCTTGCAT CAACGGAAAA 
CGCATGTCCG AGTACCGATC ATGGCGTCAG GTGAAGCTGG AGATCAAGGG AGATCTGCTG 
CGTATCTACT CAGGCCGACA TGCCAAATCG GAGCACAATG TGATAGAACT TGATATTAGA 
AACTTTAAGT TCTTCGACGA GTCGATGGAT AAGAAGAAAT ACTTGATCCG TATGCAGTCG 
AAGCCATCGC CCAGTGGAGC CCATTTGGCC TCCTTGGGCA ACGAGTTAAA CCTGGACGAG 
GGTCACGACA AGCTGCTAAC CTCGTCGGGC AGCAGCAGTG CGAATGAGCC CGCTGCCAAC 
GCAGCATCGG CCCTGACCGC ATCCACCTCC TCGTCCACCT GCAGCAGCGG TCCTTACACG 
GAGATCCTAT TCAAGACGAA GAGCAGCAAC GAGATGAAGC GTCTGTTTGG ACTTCTGCAG 
TGGAAGGATA GCCTCAACTA CGATGATAAT GAGCACCAGC CACAGGGCAA GCAGTTGGCA 
GAACCACAGA AAACAGTGGC CAGCGCCAGT TATCTGGACG ACGTCCAAGG TGGAGCGAGT 
GCAGCCGACA GCTCACCCTT GAGCTCCCAT TCCGGTGGCA TTGCAGACGC TAGTGGCAGT 
CATCATGTTT CTGCGCTACC AGCAGCTGCG GCTATTGTCG CCGCCACCAG CGACTCCATT 
TCGCCGGTGA TGAAGGCAAG GAAGTCCTCT TCGCACAAGC ATATACCTGA TAAGGATTTG 
GGATCGCCCA AATCGAAGAA CTGGAAGGAT TTGCTCTTCC GCCGTGGCGG CAGTGGCAGC 
GGAGGAGTGT CACACCACGA CTTGGCTTCG CCATCCGCCT GTGCAGCCAA GCAGATTGGA 
TCTATTGGCG TTCCACTGCG CAGCTGTCCC ATGTCGAAGG TCAATGCATA TGTGCCGCAT 
CTGGTAGAGG TGTGCACGAA CATTGTGGAG ACAAAGGGTC TGGGAGTGGT GGGCATCTAT 
CGCATACCCG GCAACAAGGC GGCCATATCA GAGCTGTCCG AACTTGTTAA CACCAAGGAC 
TTTCAGTTCG AGAGCTGTGC CAGCGATGAC CGCTGGGAGG ATGTGAATGT GGTGAGCAGC 
CTGCTCAAGT TGTTTATACG CAGCTTGCCC GATGCCTTAA TGCCGGCAAG TTACTATATC 
AACTTTATTG AGGCGGACAA GAAGTTTGGC CTGGAGAGAA TTGTACTGTT GCGCGAGATT 
GTGGAATCGT TACCACGGCA TCCCTACGAA ACGATGAAGC ACTTGATTCG GCATCTGTGC 
CGAGTTAGCG GCAATTGCGA TGTGAACCGC ATGGAGCCCA AGAACCTGGC CATCATATTT 
GGGCCATCTA TAATCAGGAC GCCGAACGAT ACTCTGGAAA CGGCAGTCAA GGACATGAAG 
CATCAATGCC GCATCGTTGA GCTGCTGGTC ACACAGTACG ACTACTTCTT CGAAGGCGGC 
AGTCTGCCGG ATATAGCCGA AGTAAGCGGC GGTACAGCTG CCGTAAGCGC CGCTCAGCCG 
CAGCAGCAGA CGCAGGAGGA TCAGACGAGC ATGCTGCTGC ACAACCTGTC GAAGATCGAG 
CGGATCACAG AGCGCGAAAC CACGCGCACC TCGCGCTTTA TGCCGCAGCT CAGACGAAAG 
ACCCATGGCA AGCGCAGCGC CGTAAACTCG GACACGTACT CCGGCGAGAG CGTTCTGGTA 
AGCGGGAGCT CCTCCAACAC CACCACCACC ACCACCACCA CCACCCACAC CACAAGTAGC 
ACAAGTAGTA GCAACACCAT CACCACAACC ATCAGCACCA CCACCATCCA GCAGCGACGG 
GCGCAGCGCA AGGCTGTGCA GAGCATTTGC GACCAGGCTC TAATCCTGCT CCTGCCCACA 
CCCGTTTCTT CCGTCTCTCT TCCCTTTCAG TCGTTGGACT ACGCCAAGGC GTCCGCCTCT 
GCCTCGGCAT CCGCAAGCAG CACCGCCAGT ACTTCGAGCA GCTCGACGCT TCACAGCGGG 
GGCAAGGCAT CCGCCTCATC CTCATCCAGT TCCACTACCA AGTTCAGCTC ATCGTCATCG 
TCGGCGTGCT CAACAACGGC GGTGGCTATT TCTTCCTCCA TCTCGGCGAC GTCCTCCAAA 
TTGTTAAGCG CCAATTTTAA GAAGCGTAGC ACAGGAGGTT TCTTGGGCTC AAGATCCTCG 
CACCAGACGC GCAAGGCCAG CTTGGACGAA AAGGACTCAG GACAGTCGTC TGGCAGCCTT 
GGTCACAGCC TGGGAGTAGG CATAAATCTG GCCAAGGAAC AACAGCGCAG CAGCGTGGAC 
ATATCCATTC TGCTAACGGA CGACGACTCT AGCAGCAGGC TAAGCGATAC GGGTTCCATG 
TCGCTGACGA CCATTACGGA TACACTGGAC AGCAAGCTAC GCAACTTGCG CAGCGGCTCC 
GAATCGAATG ATGAGAATGG ATCGCCCGAG TTTCCCAAGA ACCGGCGGCA CACCTTGGGC 
CAGCCTCTGC ACATGCACTC CGAGAACATT CCGTATGCGG ACGAGTCGCC CGAGCGGCTG 
TTCCATCAGT TTTGCAATCC CCTGGAGACA GCCCCCTTAT CCCTGCATCT AAGCCGCTCC 
TGCACGGCCA CAAATACAAT TGGTGGCGAT GAAAAGCCTT CGAAACAGTC GGGTGGAGTT 
GCACCACTGC CCCCAAGTTT GCTTAAGGCC GCACATAAGC TACAGCACTC AGCCACGGTG 
CCCATTCATC AGGGAAGCTC CACTGCACCA ACTAGCGGAG CAGCCACGCC AGTAGGCACG 
CCAACCGGAA GCGGTGGGGC CGGAGCTATT CCCTCGCGCA GCTCATCGAG CAGTGTCACG 
ACGCTAACCA AAAGCATACA ACCCAGGTTC AGTAACTATC TAAGCTACGA AAGAGGCAAT 
GCAGCCGCTG GAGGCGCCGA AAGGGTGGTG GGATCCGCCG GAGGAGCTGC CTCCGACGAC 
GATTCCGAGG GCTCGACCAC CAGTGATCCC AAGGAGAAGC TGCTTCTCGG TGAACGACCG 
TCGCCTTCGT TTTTTATCGA GCGCTACAAC AAAAAGAGAC GCGATCATCG GTTATTTCGC 
AGCGCCAGTT TCAATTGCCG GAACTACTCC ACCAGGCACA TAACGGCGCA GACATCCGGA 
GGAGTAGCGG CGGGAGTATC GGGCGGAGTA GCTGGAGCGG TGGCGGTGGG GGTGGCTTGC 
TGCCAGGCAT TGAGCACCAC TGGCTTGACC AAGGACGAGA AGACTGATAT GAACCTGACC 
AAGAAGCGCC AGATCCAGAA CAAGCAGAAT CGGTCCATTA AGCGGCGGCA CACAGTAGGC 
GGACCTCACG ACTATTCCGC CAGCAATGGT GGCTGCTCGA ATCACTCACA CGGTCACGAC 
CTGGCAGCCA TCAACTACAA TCACCACAAT CGCCATCACC AGCAACAGTT GTTGAAACTG 
GGCAGCGCTA CATCTGGCGG TGGAGGCAGT GGCGCAGCCG CGGCCGAGAC GACGACCACC 
ACAACGACCA CCACCACGGT GCTGCGGCGC CTCGGTACAG GTCAAGCTGG CGCCGAAGCT 
AGTGTGGCAT TGCCCGCATC AAGTTCCGGA TCGAGCAGCA ACAACAATAA CTCGCCCCAA 
AGCGATGGCA GTAATGGCAA CGGCGGTGGA GTGGCGGGAG TTGCCTCCAA TGCACCACCA 
ACTGGCGTAG GTGTCGGTGG CAACAACAGC AGCGGAAGCA GCAGCAACAG CAGCAGCACC 
AGCACAACTC CCGCTGGGAA CGGCGATCAG GTCCTGGAAG TGGGCATACG TATCAAGAAC 
ATTAGCCGGG GTCGCTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285496.1
CDS	803..7423
Translation	MLQNSNGAAAAHSAASAVATPAAAASAAAAAAAAAVAAAANNAALAASSIQPKLIVIRRRPNQGFGFTLRHFIAYPPEDDQASSSASGLVSGSATAATAASVSTNWPQEASSAAGSNSGSSSSVGVAGITGLEPTSPTSLPPYQVKAMETIFIKEVQANGPAHYANLQTGDRVLMVNNQPIAGIAYSTIVSMIKQTPAVLTLHVVPKECDVLQMHYTSIAHTPESNRLTMSTHSPSLLANGSHKTTFPAAAAVAAPPHQHQQQQQQLISYPAVAAVTTSTPAGSPQSQSQSQSHSHPNTHHAPSLHGGSRSGSITSTASGGITVLSQPFYPQQQQQQQQQQQHRHPALTGGSSSIDFGDMAHGLRQHQQQQQHLYQQQQQHHQFMRANQPPNLTVLSASSATNTPTPTARQPKSATSHNQALYAVGAGPSSAAGSGSGSLSGGSENSDLMIRLRESIKQKEEFLKSPVPASMSSASSINGNQAQSSSPAQQQHLQQHQQHFFPSHTPPGGVQVVVPPPRSRHQVMLKQQQQQQQQHQQHHVLGYDMYHSGGKLVQRSSTAPGSVCHQHQQQQQHHLYHQQQHQQLQQTQHRQVMINNNAKYAKDLDYFETLQETGVTNKVFERKLVSHMSKGFDPFKPLSINTSALESSASTSSTSATTTVKKQSVLYESLQQHPLAKYHQTQQQTTTTTTVDGQTSSRMELHKATLANATIDPVPVGSSKFVTLPDKPDKGSSVATLADIAESSAAAIVDSASGSGNVVRRYKPLSSSSFDEGKPMRRISYLRATNNETDFNAEAAAGGDAAATGASGPASASAASSAPGPTPSSAPAPAPAPAPLPISIPIPVAAVVEPQKTKSLVEEHPDPTYDVIGGTGGGHEFIETPNGLEDVRLSAHGSRRASMSSDMRSSIHIDAELNGKYVPNELEAFETEIEIKSSCINGKRMSEYRSWRQVKLEIKGDLLRIYSGRHAKSEHNVIELDIRNFKFFDESMDKKKYLIRMQSKPSPSGAHLASLGNELNLDEGHDKLLTSSGSSSANEPAANAASALTASTSSSTCSSGPYTEILFKTKSSNEMKRLFGLLQWKDSLNYDDNEHQPQGKQLAEPQKTVASASYLDDVQGGASAADSSPLSSHSGGIADASGSHHVSALPAAAAIVAATSDSISPVMKARKSSSHKHIPDKDLGSPKSKNWKDLLFRRGGSGSGGVSHHDLASPSACAAKQIGSIGVPLRSCPMSKVNAYVPHLVEVCTNIVETKGLGVVGIYRIPGNKAAISELSELVNTKDFQFESCASDDRWEDVNVVSSLLKLFIRSLPDALMPASYYINFIEADKKFGLERIVLLREIVESLPRHPYETMKHLIRHLCRVSGNCDVNRMEPKNLAIIFGPSIIRTPNDTLETAVKDMKHQCRIVELLVTQYDYFFEGGSLPDIAEVSGGTAAVSAAQPQQQTQEDQTSMLLHNLSKIERITERETTRTSRFMPQLRRKTHGKRSAVNSDTYSGESVLVSGSSSNTTTTTTTTTHTTSSTSSSNTITTTISTTTIQQRRAQRKAVQSICDQALILLLPTPVSSVSLPFQSLDYAKASASASASASSTASTSSSSTLHSGGKASASSSSSSTTKFSSSSSSACSTTAVAISSSISATSSKLLSANFKKRSTGGFLGSRSSHQTRKASLDEKDSGQSSGSLGHSLGVGINLAKEQQRSSVDISILLTDDDSSSRLSDTGSMSLTTITDTLDSKLRNLRSGSESNDENGSPEFPKNRRHTLGQPLHMHSENIPYADESPERLFHQFCNPLETAPLSLHLSRSCTATNTIGGDEKPSKQSGGVAPLPPSLLKAAHKLQHSATVPIHQGSSTAPTSGAATPVGTPTGSGGAGAIPSRSSSSSVTTLTKSIQPRFSNYLSYERGNAAAGGAERVVGSAGGAASDDDSEGSTTSDPKEKLLLGERPSPSFFIERYNKKRRDHRLFRSASFNCRNYSTRHITAQTSGGVAAGVSGGVAGAVAVGVACCQALSTTGLTKDEKTDMNLTKKRQIQNKQNRSIKRRHTVGGPHDYSASNGGCSNHSHGHDLAAINYNHHNRHHQQQLLKLGSATSGGGGSGAAAAETTTTTTTTTTVLRRLGTGQAGAEASVALPASSSGSSSNNNNSPQSDGSNGNGGGVAGVASNAPPTGVGVGGNNSSGSSSNSSSTSTTPAGNGDQVLEVGIRIKNISRGRF

Target ORF information:

RefSeq Version	NM_001298567.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho GTPase activating protein at 19D (RhoGAP19D), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298567.1

ORF Insert Sequence:

ATGTTACAAA ACTCGAACGG CGCTGCCGCA GCGCATTCGG CAGCCTCAGC GGTGGCGACT 
CCGGCAGCGG CGGCATCCGC AGCAGCAGCA GCTGCTGCAG CGGCGGTGGC TGCAGCGGCC 
AACAACGCCG CCCTGGCCGC CTCCTCCATC CAGCCCAAGC TCATCGTCAT CCGCCGGCGA 
CCCAACCAGG GATTCGGGTT CACGCTGCGC CACTTCATCG CCTATCCGCC GGAAGATGAC 
CAGGCATCAT CATCCGCATC GGGTTTGGTA TCGGGATCGG CGACAGCAGC AACGGCGGCA 
TCGGTATCGA CCAACTGGCC ACAGGAAGCC TCCAGTGCGG CGGGCAGCAA CAGCGGCAGC 
AGCAGCAGCG TCGGCGTCGC TGGGATCACC GGTTTGGAGC CAACGTCGCC GACGTCGCTG 
CCGCCGTACC AAGTGAAAGC GATGGAGACC ATTTTCATCA AAGAGGTGCA GGCGAACGGA 
CCGGCCCACT ACGCCAACCT GCAGACGGGC GACCGCGTGC TGATGGTCAA CAACCAGCCG 
ATCGCTGGCA TCGCCTACAG CACCATCGTC TCGATGATCA AGCAGACGCC GGCGGTCCTC 
ACGCTGCACG TGGTGCCCAA GGAGTGCGAT GTCCTGCAGA TGCACTATAC GAGCATTGCC 
CACACGCCGG AGTCGAATCG CCTGACCATG TCGACGCACT CGCCCTCGCT GCTGGCCAAC 
GGCTCCCACA AGACCACATT TCCAGCGGCG GCAGCAGTTG CAGCACCACC GCATCAGCAT 
CAGCAGCAGC AGCAACAGTT GATCTCATAT CCCGCTGTTG CAGCCGTGAC CACATCAACG 
CCGGCAGGAT CGCCGCAATC CCAATCCCAA TCCCAATCGC ACTCGCACCC CAACACCCAT 
CATGCACCAT CCCTGCACGG AGGCAGTCGA TCGGGCAGCA TCACCAGCAC GGCCAGCGGA 
GGCATCACCG TCCTCAGTCA GCCGTTCTAC CCACAGCAGC AGCAGCAACA GCAACAGCAG 
CAGCAACATC GTCATCCAGC GCTGACTGGC GGCAGCAGCA GCATCGATTT CGGGGACATG 
GCACATGGAC TGCGCCAGCA TCAGCAGCAG CAGCAACATC TGTACCAGCA GCAGCAACAG 
CATCACCAGT TTATGCGTGC TAATCAGCCG CCAAATTTAA CAGTATTATC GGCCAGCTCG 
GCAACGAACA CGCCCACACC CACCGCCCGC CAGCCCAAGT CCGCGACGAG CCACAATCAG 
GCGTTGTATG CAGTCGGAGC GGGTCCGAGC TCAGCAGCTG GATCTGGGTC GGGATCCCTT 
TCGGGGGGCA GTGAGAACAG CGACCTTATG ATCCGACTGC GCGAGTCCAT CAAGCAGAAG 
GAGGAGTTCC TTAAGTCGCC GGTGCCCGCC TCCATGAGCT CCGCCTCCTC TATCAACGGC 
AACCAGGCGC AGTCGTCATC GCCGGCACAG CAGCAACACC TGCAGCAGCA CCAGCAGCAC 
TTCTTTCCCT CGCACACGCC GCCAGGTGGT GTCCAAGTGG TGGTGCCTCC ACCGCGCAGT 
CGCCATCAAG TGATGCTCAA GCAGCAGCAG CAGCAACAGC AGCAGCACCA GCAACATCAT 
GTCCTTGGCT ACGACATGTA CCACTCCGGT GGTAAGCTGG TGCAGCGGTC TTCCACGGCG 
CCTGGCAGTG TGTGCCACCA ACACCAGCAG CAGCAGCAGC ATCACCTCTA TCACCAGCAG 
CAACATCAGC AGCTCCAGCA GACGCAACAT CGCCAGGTGA TGATCAACAA CAATGCCAAG 
TACGCCAAGG ATCTGGACTA TTTTGAGACG TTGCAGGAGA CAGGAGTCAC CAATAAGGTC 
TTTGAGCGCA AGCTGGTGTC ACACATGTCC AAGGGATTCG ATCCGTTTAA GCCGCTGTCG 
ATCAACACCA GTGCCCTGGA GTCGTCTGCC AGCACGTCGA GCACTTCGGC CACCACGACG 
GTGAAGAAGC AAAGTGTGCT GTATGAATCC CTGCAGCAGC ATCCATTGGC CAAGTACCAT 
CAGACGCAGC AGCAAACGAC AACGACGACG ACGGTGGATG GACAGACGAG TAGCCGGATG 
GAGCTGCACA AGGCGACCCT GGCGAATGCC ACCATCGATC CGGTGCCAGT TGGATCCAGC 
AAGTTTGTGA CGCTGCCGGA TAAGCCGGAT AAGGGTTCCT CCGTTGCCAC CTTGGCGGAC 
ATCGCCGAGA GCAGTGCAGC AGCCATTGTG GACAGTGCGT CGGGAAGCGG CAACGTTGTG 
CGGCGCTACA AGCCATTATC CTCCAGCTCC TTTGACGAAG GCAAACCAAT GCGTAGGATA 
TCCTACCTGC GGGCAACCAA CAATGAGACC GACTTCAATG CGGAGGCTGC CGCTGGCGGA 
GATGCCGCGG CAACAGGGGC GTCAGGACCA GCATCTGCAT CAGCAGCTTC ATCTGCACCT 
GGACCCACGC CCTCATCTGC TCCTGCACCA GCTCCAGCAC CAGCACCCCT GCCCATTTCG 
ATCCCCATTC CGGTGGCTGC TGTCGTCGAG CCGCAAAAGA CGAAGTCCCT GGTGGAGGAG 
CATCCGGATC CCACCTACGA TGTGATCGGA GGCACAGGTG GTGGCCACGA GTTCATCGAG 
ACGCCGAACG GCCTGGAGGA TGTGCGTCTG TCGGCCCACG GGAGTCGGAG GGCCTCCATG 
AGCAGTGACA TGCGAAGCAG CATCCACATT GATGCCGAGT TAAATGGCAA GTATGTGCCA 
AACGAACTGG AGGCCTTTGA GACGGAAATC GAGATCAAGT CCTCTTGCAT CAACGGAAAA 
CGCATGTCCG AGTACCGATC ATGGCGTCAG GTGAAGCTGG AGATCAAGGG AGATCTGCTG 
CGTATCTACT CAGGCCGACA TGCCAAATCG GAGCACAATG TGATAGAACT TGATATTAGA 
AACTTTAAGT TCTTCGACGA GTCGATGGAT AAGAAGAAAT ACTTGATCCG TATGCAGTCG 
AAGCCATCGC CCAGTGGAGC CCATTTGGCC TCCTTGGGCA ACGAGTTAAA CCTGGACGAG 
GGTCACGACA AGCTGCTAAC CTCGTCGGGC AGCAGCAGTG CGAATGAGCC CGCTGCCAAC 
GCAGCATCGG CCCTGACCGC ATCCACCTCC TCGTCCACCT GCAGCAGCGG TCCTTACACG 
GAGATCCTAT TCAAGACGAA GAGCAGCAAC GAGATGAAGC GTCTGTTTGG ACTTCTGCAG 
TGGAAGGATA GCCTCAACTA CGATGATAAT GAGCACCAGC CACAGGGCAA GCAGTTGGCA 
GAACCACAGA AAACAGTGGC CAGCGCCAGT TATCTGGACG ACGTCCAAGG TGGAGCGAGT 
GCAGCCGACA GCTCACCCTT GAGCTCCCAT TCCGGTGGCA TTGCAGACGC TAGTGGCAGT 
CATCATGTTT CTGCGCTACC AGCAGCTGCG GCTATTGTCG CCGCCACCAG CGACTCCATT 
TCGCCGGTGA TGAAGGCAAG GAAGTCCTCT TCGCACAAGC ATATACCTGA TAAGGATTTG 
GGATCGCCCA AATCGAAGAA CTGGAAGGAT TTGCTCTTCC GCCGTGGCGG CAGTGGCAGC 
GGAGGAGTGT CACACCACGA CTTGGCTTCG CCATCCGCCT GTGCAGCCAA GCAGATTGGA 
TCTATTGGCG TTCCACTGCG CAGCTGTCCC ATGTCGAAGG TCAATGCATA TGTGCCGCAT 
CTGGTAGAGG TGTGCACGAA CATTGTGGAG ACAAAGGGTC TGGGAGTGGT GGGCATCTAT 
CGCATACCCG GCAACAAGGC GGCCATATCA GAGCTGTCCG AACTTGTTAA CACCAAGGAC 
TTTCAGTTCG AGAGCTGTGC CAGCGATGAC CGCTGGGAGG ATGTGAATGT GGTGAGCAGC 
CTGCTCAAGT TGTTTATACG CAGCTTGCCC GATGCCTTAA TGCCGGCAAG TTACTATATC 
AACTTTATTG AGGCGGACAA GAAGTTTGGC CTGGAGAGAA TTGTACTGTT GCGCGAGATT 
GTGGAATCGT TACCACGGCA TCCCTACGAA ACGATGAAGC ACTTGATTCG GCATCTGTGC 
CGAGTTAGCG GCAATTGCGA TGTGAACCGC ATGGAGCCCA AGAACCTGGC CATCATATTT 
GGGCCATCTA TAATCAGGAC GCCGAACGAT ACTCTGGAAA CGGCAGTCAA GGACATGAAG 
CATCAATGCC GCATCGTTGA GCTGCTGGTC ACACAGTACG ACTACTTCTT CGAAGGCGGC 
AGTCTGCCGG ATATAGCCGA AGTAAGCGGC GGTACAGCTG CCGTAAGCGC CGCTCAGCCG 
CAGCAGCAGA CGCAGGAGGA TCAGACGAGC ATGCTGCTGC ACAACCTGTC GAAGATCGAG 
CGGATCACAG AGCGCGAAAC CACGCGCACC TCGCGCTTTA TGCCGCAGCT CAGACGAAAG 
ACCCATGGCA AGCGCAGCGC CGTAAACTCG GACACGTACT CCGGCGAGAG CGTTCTGGTA 
AGCGGGAGCT CCTCCAACAC CACCACCACC ACCACCACCA CCACCCACAC CACAAGTAGC 
ACAAGTAGTA GCAACACCAT CACCACAACC ATCAGCACCA CCACCATCCA GCAGCGACGG 
GCGCAGCGCA AGGCTGTGCA GAGCATTTGC GACCAGGCTC TAATCCTGCT CCTGCCCACA 
CCCGTTTCTT CCGTCTCTCT TCCCTTTCAG TCGTTGGACT ACGCCAAGGC GTCCGCCTCT 
GCCTCGGCAT CCGCAAGCAG CACCGCCAGT ACTTCGAGCA GCTCGACGCT TCACAGCGGG 
GGCAAGGCAT CCGCCTCATC CTCATCCAGT TCCACTACCA AGTTCAGCTC ATCGTCATCG 
TCGGCGTGCT CAACAACGGC GGTGGCTATT TCTTCCTCCA TCTCGGCGAC GTCCTCCAAA 
TTGTTAAGCG CCAATTTTAA GAAGCGTAGC ACAGGAGGTT TCTTGGGCTC AAGATCCTCG 
CACCAGACGC GCAAGGCCAG CTTGGACGAA AAGGACTCAG GACAGTCGTC TGGCAGCCTT 
GGTCACAGCC TGGGAGTAGG CATAAATCTG GCCAAGGAAC AACAGCGCAG CAGCGTGGAC 
ATATCCATTC TGCTAACGGA CGACGACTCT AGCAGCAGGC TAAGCGATAC GGGTTCCATG 
TCGCTGACGA CCATTACGGA TACACTGGAC AGCAAGCTAC GCAACTTGCG CAGCGGCTCC 
GAATCGAATG ATGAGAATGG ATCGCCCGAG TTTCCCAAGA ACCGGCGGCA CACCTTGGGC 
CAGCCTCTGC ACATGCACTC CGAGAACATT CCGTATGCGG ACGAGTCGCC CGAGCGGCTG 
TTCCATCAGT TTTGCAATCC CCTGGAGACA GCCCCCTTAT CCCTGCATCT AAGCCGCTCC 
TGCACGGCCA CAAATACAAT TGGTGGCGAT GAAAAGCCTT CGAAACAGTC GGGTGGAGTT 
GCACCACTGC CCCCAAGTTT GCTTAAGGCC GCACATAAGC TACAGCACTC AGCCACGGTG 
CCCATTCATC AGGGAAGCTC CACTGCACCA ACTAGCGGAG CAGCCACGCC AGTAGGCACG 
CCAACCGGAA GCGGTGGGGC CGGAGCTATT CCCTCGCGCA GCTCATCGAG CAGTGTCACG 
ACGCTAACCA AAAGCATACA ACCCAGGTTC AGTAACTATC TAAGCTACGA AAGAGGCAAT 
GCAGCCGCTG GAGGCGCCGA AAGGGTGGTG GGATCCGCCG GAGGAGCTGC CTCCGACGAC 
GATTCCGAGG GCTCGACCAC CAGTGATCCC AAGGAGAAGC TGCTTCTCGG TGAACGACCG 
TCGCCTTCGT TTTTTATCGA GCGCTACAAC AAAAAGAGAC GCGATCATCG GTTATTTCGC 
AGCGCCAGTT TCAATTGCCG GAACTACTCC ACCAGGCACA TAACGGCGCA GACATCCGGA 
GGAGTAGCGG CGGGAGTATC GGGCGGAGTA GCTGGAGCGG TGGCGGTGGG GGTGGCTTGC 
TGCCAGGCAT TGAGCACCAC TGGCTTGACC AAGGACGAGA AGACTGATAT GAACCTGACC 
AAGAAGCGCC AGATCCAGAA CAAGCAGAAT CGGTCCATTA AGCGGCGGCA CACAGTAGGC 
GGACCTCACG ACTATTCCGC CAGCAATGGT GGCTGCTCGA ATCACTCACA CGGTCACGAC 
CTGGCAGCCA TCAACTACAA TCACCACAAT CGCCATCACC AGCAACAGTT GTTGAAACTG 
GGCAGCGCTA CATCTGGCGG TGGAGGCAGT GGCGCAGCCG CGGCCGAGAC GACGACCACC 
ACAACGACCA CCACCACGGT GCTGCGGCGC CTCGGTACAG GTCAAGCTGG CGCCGAAGCT 
AGTGTGGCAT TGCCCGCATC AAGTTCCGGA TCGAGCAGCA ACAACAATAA CTCGCCCCAA 
AGCGATGGCA GTAATGGCAA CGGCGGTGGA GTGGCGGGAG TTGCCTCCAA TGCACCACCA 
ACTGGCGTAG GTGTCGGTGG CAACAACAGC AGCGGAAGCA GCAGCAACAG CAGCAGCACC 
AGCACAACTC CCGCTGGGAA CGGCGATCAG GTCCTGGAAG TGGGCATACG TATCAAGAAC 
ATTAGCCGGG GTCGCTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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RhoGAP19D cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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