zld cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	zld
Entrez Gene ID	32994
Full Name	zelda
Synonyms	CG12701,Dmel\CG12701,EP1344,VFL,Vfl,ZLD,Zelda,Zld,l(1)G0225,l(1)G0353,l(1)G0427,vfl
General protein information	Preferred Names zelda Names CG12701 gene product from transcript CG12701-RD CG12701-PA CG12701-PB CG12701-PD CG12701-PF CG12701-PG EP1344 ZELDA lethal (1) G0225 lethal (1) G0353 lethal (1) G0427 vielfaltig vielfaltig/Zelda zinc-finger early drosophila activator zld-PA zld-PB zld-PD zld-PF zld-PG
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 18F-18F

mRNA and Protein(s)

mRNA	Protein	Name
NM_001258839.2	NP_001245768.1	zelda, isoform F
NM_167685.3	NP_728304.1	zelda, isoform B
NM_134512.5	NP_608356.1	zelda, isoform A
NM_001298537.1	NP_001285466.1	zelda, isoform G
NM_001258840.3	NP_001245769.1	zelda, isoform D

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our results unveil new roles of zld in different biological contexts and suggest that changes in expression of zld (and probably other major TFs) are critical in the evolution of insect Gene regulatory networks
Title: Evolution and multiple roles of the Pancrustacea specific transcription factor zelda in insects.

we identify key roles for the Zelda and Zerknullt transcription factors in establishing the resulting expression domain, and find widespread binding of insulator proteins to the Mad and Brinker-bound genomic regions. Analysis of embryos lacking the BEAF-32 insulator protein shows reduced transcription of a peak BMP target gene and a reduction in the number of amnioserosa cells, the fate specified by peak BMP signaling.
Title: Regulation of the BMP Signaling-Responsive Transcriptional Network in the Drosophila Embryo.

both Zelda and GAGA factor function to specify sites of open chromatin and together facilitate the remodeling of the early embryonic genome
Title: Zelda is differentially required for chromatin accessibility, transcription factor binding, and gene expression in the early Drosophila embryo.

Zld primes enhancers by lowering the high nucleosome barrier just enough to assist transcription factors (TFs) in accessing their binding motifs and promoting spatially controlled enhancer activation if the right patterning TFs are present.
Title: Zelda overcomes the high intrinsic nucleosome barrier at enhancers during Drosophila zygotic genome activation.

Zelda binds DNA using C-terminal zinc fingers and activates transcription through a low-complexity domain.
Title: Transcriptional activation is a conserved feature of the early embryonic factor Zelda that requires a cluster of four zinc fingers for DNA binding and a low-complexity activation domain.

The following zld gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the zld cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa02182	NM_001298537.1 Latest version!	Drosophila melanogaster zelda (zld), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OFa02182	NM_134512.5 Latest version!	Drosophila melanogaster zelda (zld), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OFa02182	NM_167685.3 Latest version!	Drosophila melanogaster zelda (zld), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OFa03176	NM_001258840.3 Latest version!	Drosophila melanogaster zelda (zld), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OFa21549	NM_001258839.2 Latest version!	Drosophila melanogaster zelda (zld), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

zld ( NM_167685.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_728304.1 Drosophila melanogaster zelda (zld), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa02182
Clone ID Related Accession (Same CDS sequence)	NM_167685.3 , NM_134512.5 , NM_001298537.1
Accession Version	NM_167685.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	zelda, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_167685.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGAGCA TTAAGACCGA GATGCCGCCC CTGCACGCGG CAGAGGCGCT CGCCTCCAGC 
AGTGCTACAG ACAGTGGTGG GGGAGGAGCA GGAGGAGGAG GAGGAGGAGG TGGAGGAGGA 
TCGGGCGGAC CAGGAGCGGG AGGAACGGGA GGCGTAGGCA GTGCGCCCGC CACACCCAAT 
GCCACAATTA GCGCCGCCGC CGACTCCAGT GATAATCAAC CCGGCACGCC GCAGCCCACG 
CAACAGCAGC AATCGACGCA GCAACAGTTG CAGCAGCCAC AATCGCAACA GCAGCAGCAG 
CAGGCCATGG GTGGAGGAGA TCCCCAGCAG CAGCAACAGC AGCAGCAGGT AACGGGTATC 
ACCCACCAGC CGTACGCCAC CCACCACATG TACTCCGCAT CTGGCGGACA GCAGCAACAA 
CAGCAGCAGA TCTACGGCGG ACTCTACGGC GGGGACATGC AGCAGCAGCA GGGTTACGCC 
AGCAGCTACA TCAACAGCTA CGAGCAGTTC TACCAGCAGC AACAGCAGCA GCAGCAGGGC 
ACTGATTACG CCTTTGGCAC CGTGGGCGTT GACTATGGCA AGAGCGCCGG TGTTCGCTAT 
CATCCCTACC TGCAGACGCC AACCTCCGGA CTGGGAGGCA TTCCAACGGC CAGCTCGGCA 
CAGGAGGAGG CGGGCAGTGC GCCCAGCGCG GTCAGCACCA CCACAGCGGT GGCCATGAGT 
CCGCGTGTGG TTAGCAGCAG CAGTCCAACG TCGACGTCCT CGCACCTGCA GCTGGGCAGC 
TCCAGTGGCC AGACACCAGG CTCACCAGGC GGAGCCGCCG GAAGTGCCGG ATGCAAGTTG 
CAGTGCAAGA AGTGCGGCAT CCTCACCACC AACGAGTCGG AGCTACAGGA GCACATCGCC 
AACGTGCACG GAGAGTCGCC GTACGGCAGC AGCGGTTACG CCAGTTCACC GTACATCAAG 
GAGGAGATGC CCACACCACA GCCACCCGGC GTTGGATCCG CAACGGCCAA TCCCGGCGAA 
TTACTCGACC TAGATTCCCA GAAGATGGTC TATCACCAGC AGCTGCTCCA GCAGCAACAA 
CAGCAACACG ACGTGGTCGC TGGACTGCCG CTGGGCGCTC TGCCGGATCC GCTGCACTCG 
ATGCAATCTA TGCAGCAGCG CGCACTCCAC AGCTGGGAGC AGCAGCCACA GCAGACGGTG 
GCCTCCGTGG AGGGCCTGCC ACCCTACATG CAGCAGGGAT TGGGCGTGGG GCTGGGAGTC 
GGTGTGGACA AGTCGCCCTA CTACTCACCA AAGCAGTCAC CCTATCATCA GTCAACCGGC 
GTTGGCGGAG CCACGCTGAT CAAGCAGGAG TACGGCGGCC ACGGACTGAT CAAGTCCGAG 
TATCCCGATT CGCAGCATTA CGTGGACAAG TCCTTCGATC CCACAGCTGG CGGAGCTGGT 
GGCGCGGAAC TGTGCGCCAG CGTGGCCACC AGTCCGGCGG AGTTCCCCAG CACCACTACG 
GGCGGACCAG GGCAGGAGGG AGCGGCGGGT GCGGCGCCAG GAGGTGGATA CCGCGGTTTC 
GAGCCACCCA GCTCGAGCTC CGTGCTGCCG GCCAACAGCC TGACGGCCAA GGCGGCCACC 
TGGAAGTCGA ACGAGGCGCG CCGTCCGAAA ACGTACAACT GCACGGCCTG CAACAAGTGG 
TTCACCAGCT CGGGCCATCT TAAGCGCCAC TACAATACGA CGCTGCACAA GAATGCGGTT 
AAGTCGAGCG GCCAGCCGGA TCCAGCCACC CTGCCCATCT CGGCGCACCA CCATCCCGCC 
CGCGACCCGG TGACGAAGCC CAGTCGCAGG GGCAATGCCG CAGCCGCTGC GGCCGCCGCT 
GCAGCTGCCG CCTCGGCCAG CGGTCAGGGT CAGCAGCAAC AGCCGCCGAT ACCGCCGCCG 
CCGGCCAATG TGCCACCCCC GGAGCCGCCT AGAAGTCCGC CCGATTATGG CGGAGGAGGC 
GGCTTGGGCG TGGGAGCGAT GGGCGGCGCT GCCATGTCCC AGTACTCGGC CTCACCCTCG 
CCCACACAGC AGCAGCAGCA CCACCTCAAC CACCATCAGC AGCAGGCCAA CGGCTATGCT 
AATGGCACCG CCAACGGCTA TGGCTACATG CAGCAACAAG TGCAATCCAC GACAAACGCT 
TCACCACAGC ACGCTTCCAA CAACAACAGC AACAACCAGC AGCAGCAGCA ACAACAGCAG 
CAGCACCATC AGCAGCAGAT GCCGCAGCAC CACAACTCCG TTTTGAACGG TCACCCAAAC 
GGGCTAGCAG GTCCCTCCGC CCCACACAAC AACAACACCA CCCAAATGCC GTCCTCCCAA 
ATGAGGGGCC TGCTGAACGA AACAACAACC ACGCCGCCAC CAACAACAAC AACCCGAGCA 
CCACAAATCA CAACAACAGC AACAACAACA ACAACGGCGG CCACCACGGT AGCTATAAAG 
AGCGAGCAAA TGGAGGACAG CAACCACACT CACACCCACA CACACACGCA TCCCAATCAC 
AGTCACAGCC AGGACAGGAG CCACAGCAGC AGCAGCAGCA GCTCAATGGC CACGGAGGAG 
GCGGAGGAGC AGGAGCTGCG GGATCAGGAG CAGGCGGACG ATCACCTGCA TCAGCATCAA 
CAGGCGTCGC AGCAGTATCT GCTGTCGGCG CGTCATTACC ACAGCAGCAC GCCCAACACG 
CTCAGCAGCA GCAACACCAA TCCCAGCACG CCCAGCAGCA ACTCGCCCCA TACTATCTAC 
CGGCAGGAGC AGCAGGGAAC GGATTTCTCG CGCACCACCC CGCCGCCGCA GCCGCTGCCG 
CCTATGGGAA TGCTGCCGCC TATGGCAATG GACTACAACA TGCTGGCTTT GGATATGCCC 
ATGCCCATGC CCACGCTCAT GCACAGCAAT ATGCTGCAGT GCAGCAGCAC CAGCACCACG 
CCGCTAGCTA CTACCATCAC CACCAGTATG CCGGACACTA TGCAGCCGCC GCAGCAGCAG 
CTGGTGCACC ACTACCAGGC GGTGCTCCAC CCGCTCCATC AGCAGCTGGG GGAGCAGCAC 
CAGCGCCAGG AGGCGGATCA TCATCAGCAG CAGCGGGAGC TTCACCAACT GGATCAGCAG 
CAGCAGCAGC AGCAGGCGTT AATCCTGGCG GACAGTTTGC CGCACAGCAG CAGTTCGCCC 
ACCAGCAGCT CCCCACCGCC CACCATGCCC ATGCCGCTCA CCACCATCAC AGCGCCGCAG 
CTGCTGCCGC TGCAGCCGCC GCCGCCGCAC ATCACCAGCA CCATGCCCAT GCCGCCCACC 
ATGCACATGC CCATCATGCC GCCGCCGCCG CAATGCTACC AGCAGCTGCA GCCGCTGGAC 
CCCACAATGA GCTATCACAC TATTATTGGT AGTGGCCCGG AGGCGCATAC CGGAACGGCC 
GGCGGTGGCT ATAGCAATCA GATCACCACC AGCGATGGCC AGATCCTGCA GCTGATGCCA 
ACATCCCTGT TCGCGCCATA TGCACCGCTC TCGCCGTACT CGGTGGCCGC CCAGCGATCG 
CCGCAGGAGG GCGATCTGCC GCCGGTTCAC ACACTCACGA CGGCCCTGCA TGCCCATCAG 
CAGGGCGGGC AGCAGGAGGC GCAGACGCCA ACGCTGACTG TGCTCTCCAC GCCCTACTCG 
CCCACAGTGA GCAGCTCGCG GGCGACTCCC GCACTGGAGA TGGATATGGC CACGCTGATG 
CAGCACCATC AGGACTATGA GATGGAGCAG TACCAGATGC AGCACCAGCA GTTGGATCAG 
ATGCAGCAGC ACCAGCAGCA ACTGGACCAC CAGCAGCAGC AACAGATCCT GGCGGATCAG 
ACCCAGACGA TGGCCCAACA GCCGCTGGCC AAGAAGCGGC GTGGCGGCAA CGCAACACCA 
TCGACGACGA AGCGACGGCG GAACAGCAGC GTCGGATCGA CGTCGCCGCA CTCGACCACC 
CTGCCATCGG GACGGATCAA GTGTCTGGAG TGCGACAAGG AGTTCACCAA GAACTGCTAC 
CTCACGCAGC ACAACAAGAG CTTCCACTCC GGTGAGTACC CATTTCGCTG CCAGAAGTGC 
GGCAAGCGCT TTCAGAGCGA GGATGTCTAC ACCACGCACC TGGGTCGCCA CCGCACCCAG 
GACAAGCCGC ATAAGTGCGA GCTGTGCCCC AAGCAGTTCC ATCACAAGAC GGATCTGCGC 
CGACACGTGG AGGCCATCCA TACGGGGCTG AAGCAGCACA TGTGCGACAT CTGTGAGAAG 
GGCTTCTGTC GCAAGGATCA TCTGCGCAAG CACCTCGAGA CGCACAATCG GCCGCGGGTG 
GTGGGCAAGA AGTCCGCCGC GGCAGCAGCC GCCGCCGCAG CAGCAGCAGC AGTCACTGCC 
ACGGCGGGAT TGGGAGGTGC ACCAGCCGCC GGTTCCATTA AGGCGGCATT TGCCCGCTCG 
CTAACGGTGA CGGCTGTGAG CAGTGAGCCA GGCGCAGGAG TTGCAATACC CTCAGCTCCT 
GCCACTGCCA CATGTGCTCC TGTATCTGTA TCAGCGTCAG CTCTTGTCCA GGCCGTCAAT 
CCCAGTCTGA CTTCCGGCCT CAGTCTGAAG CGGCACATCG ACGATGTGGC CGTTGACGAT 
GACAGCCTGT TGGAGGAGGA CGAGGATGAG ATGGAGATGG AGATGGAGAT GGATATGTTG 
GAGGAGGAAG ATGAGGAGGA GGAGGAGCTG GGCGATCACC ACATGATAAT CAAGAGCGAG 
TACGTGCAGG AGGAGTTTCA GATGATCGAG AAGAGCATAG AGCTCTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_728304.1
CDS	918..5708
Translation	MTSIKTEMPPLHAAEALASSSATDSGGGGAGGGGGGGGGGSGGPGAGGTGGVGSAPATPNATISAAADSSDNQPGTPQPTQQQQSTQQQLQQPQSQQQQQQAMGGGDPQQQQQQQQVTGITHQPYATHHMYSASGGQQQQQQQIYGGLYGGDMQQQQGYASSYINSYEQFYQQQQQQQQGTDYAFGTVGVDYGKSAGVRYHPYLQTPTSGLGGIPTASSAQEEAGSAPSAVSTTTAVAMSPRVVSSSSPTSTSSHLQLGSSSGQTPGSPGGAAGSAGCKLQCKKCGILTTNESELQEHIANVHGESPYGSSGYASSPYIKEEMPTPQPPGVGSATANPGELLDLDSQKMVYHQQLLQQQQQQHDVVAGLPLGALPDPLHSMQSMQQRALHSWEQQPQQTVASVEGLPPYMQQGLGVGLGVGVDKSPYYSPKQSPYHQSTGVGGATLIKQEYGGHGLIKSEYPDSQHYVDKSFDPTAGGAGGAELCASVATSPAEFPSTTTGGPGQEGAAGAAPGGGYRGFEPPSSSSVLPANSLTAKAATWKSNEARRPKTYNCTACNKWFTSSGHLKRHYNTTLHKNAVKSSGQPDPATLPISAHHHPARDPVTKPSRRGNAAAAAAAAAAAASASGQGQQQQPPIPPPPANVPPPEPPRSPPDYGGGGGLGVGAMGGAAMSQYSASPSPTQQQQHHLNHHQQQANGYANGTANGYGYMQQQVQSTTNASPQHASNNNSNNQQQQQQQQQHHQQQMPQHHNSVLNGHPNGLAGPSAPHNNNTTQMPSSQMRGLLNETTTTPPPTTTTRAPQITTTATTTTTAATTVAIKSEQMEDSNHTHTHTHTHPNHSHSQDRSHSSSSSSSMATEEAEEQELRDQEQADDHLHQHQQASQQYLLSARHYHSSTPNTLSSSNTNPSTPSSNSPHTIYRQEQQGTDFSRTTPPPQPLPPMGMLPPMAMDYNMLALDMPMPMPTLMHSNMLQCSSTSTTPLATTITTSMPDTMQPPQQQLVHHYQAVLHPLHQQLGEQHQRQEADHHQQQRELHQLDQQQQQQQALILADSLPHSSSSPTSSSPPPTMPMPLTTITAPQLLPLQPPPPHITSTMPMPPTMHMPIMPPPPQCYQQLQPLDPTMSYHTIIGSGPEAHTGTAGGGYSNQITTSDGQILQLMPTSLFAPYAPLSPYSVAAQRSPQEGDLPPVHTLTTALHAHQQGGQQEAQTPTLTVLSTPYSPTVSSSRATPALEMDMATLMQHHQDYEMEQYQMQHQQLDQMQQHQQQLDHQQQQQILADQTQTMAQQPLAKKRRGGNATPSTTKRRRNSSVGSTSPHSTTLPSGRIKCLECDKEFTKNCYLTQHNKSFHSGEYPFRCQKCGKRFQSEDVYTTHLGRHRTQDKPHKCELCPKQFHHKTDLRRHVEAIHTGLKQHMCDICEKGFCRKDHLRKHLETHNRPRVVGKKSAAAAAAAAAAAAVTATAGLGGAPAAGSIKAAFARSLTVTAVSSEPGAGVAIPSAPATATCAPVSVSASALVQAVNPSLTSGLSLKRHIDDVAVDDDSLLEEDEDEMEMEMEMDMLEEEDEEEEELGDHHMIIKSEYVQEEFQMIEKSIELY

Target ORF information:

RefSeq Version	NM_167685.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster zelda (zld), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167685.3

ORF Insert Sequence:

ATGACGAGCA TTAAGACCGA GATGCCGCCC CTGCACGCGG CAGAGGCGCT CGCCTCCAGC 
AGTGCTACAG ACAGTGGTGG GGGAGGAGCA GGAGGAGGAG GAGGAGGAGG TGGAGGAGGA 
TCGGGCGGAC CAGGAGCGGG AGGAACGGGA GGCGTAGGCA GTGCGCCCGC CACACCCAAT 
GCCACAATTA GCGCCGCCGC CGACTCCAGT GATAATCAAC CCGGCACGCC GCAGCCCACG 
CAACAGCAGC AATCGACGCA GCAACAGTTG CAGCAGCCAC AATCGCAACA GCAGCAGCAG 
CAGGCCATGG GTGGAGGAGA TCCCCAGCAG CAGCAACAGC AGCAGCAGGT AACGGGTATC 
ACCCACCAGC CGTACGCCAC CCACCACATG TACTCCGCAT CTGGCGGACA GCAGCAACAA 
CAGCAGCAGA TCTACGGCGG ACTCTACGGC GGGGACATGC AGCAGCAGCA GGGTTACGCC 
AGCAGCTACA TCAACAGCTA CGAGCAGTTC TACCAGCAGC AACAGCAGCA GCAGCAGGGC 
ACTGATTACG CCTTTGGCAC CGTGGGCGTT GACTATGGCA AGAGCGCCGG TGTTCGCTAT 
CATCCCTACC TGCAGACGCC AACCTCCGGA CTGGGAGGCA TTCCAACGGC CAGCTCGGCA 
CAGGAGGAGG CGGGCAGTGC GCCCAGCGCG GTCAGCACCA CCACAGCGGT GGCCATGAGT 
CCGCGTGTGG TTAGCAGCAG CAGTCCAACG TCGACGTCCT CGCACCTGCA GCTGGGCAGC 
TCCAGTGGCC AGACACCAGG CTCACCAGGC GGAGCCGCCG GAAGTGCCGG ATGCAAGTTG 
CAGTGCAAGA AGTGCGGCAT CCTCACCACC AACGAGTCGG AGCTACAGGA GCACATCGCC 
AACGTGCACG GAGAGTCGCC GTACGGCAGC AGCGGTTACG CCAGTTCACC GTACATCAAG 
GAGGAGATGC CCACACCACA GCCACCCGGC GTTGGATCCG CAACGGCCAA TCCCGGCGAA 
TTACTCGACC TAGATTCCCA GAAGATGGTC TATCACCAGC AGCTGCTCCA GCAGCAACAA 
CAGCAACACG ACGTGGTCGC TGGACTGCCG CTGGGCGCTC TGCCGGATCC GCTGCACTCG 
ATGCAATCTA TGCAGCAGCG CGCACTCCAC AGCTGGGAGC AGCAGCCACA GCAGACGGTG 
GCCTCCGTGG AGGGCCTGCC ACCCTACATG CAGCAGGGAT TGGGCGTGGG GCTGGGAGTC 
GGTGTGGACA AGTCGCCCTA CTACTCACCA AAGCAGTCAC CCTATCATCA GTCAACCGGC 
GTTGGCGGAG CCACGCTGAT CAAGCAGGAG TACGGCGGCC ACGGACTGAT CAAGTCCGAG 
TATCCCGATT CGCAGCATTA CGTGGACAAG TCCTTCGATC CCACAGCTGG CGGAGCTGGT 
GGCGCGGAAC TGTGCGCCAG CGTGGCCACC AGTCCGGCGG AGTTCCCCAG CACCACTACG 
GGCGGACCAG GGCAGGAGGG AGCGGCGGGT GCGGCGCCAG GAGGTGGATA CCGCGGTTTC 
GAGCCACCCA GCTCGAGCTC CGTGCTGCCG GCCAACAGCC TGACGGCCAA GGCGGCCACC 
TGGAAGTCGA ACGAGGCGCG CCGTCCGAAA ACGTACAACT GCACGGCCTG CAACAAGTGG 
TTCACCAGCT CGGGCCATCT TAAGCGCCAC TACAATACGA CGCTGCACAA GAATGCGGTT 
AAGTCGAGCG GCCAGCCGGA TCCAGCCACC CTGCCCATCT CGGCGCACCA CCATCCCGCC 
CGCGACCCGG TGACGAAGCC CAGTCGCAGG GGCAATGCCG CAGCCGCTGC GGCCGCCGCT 
GCAGCTGCCG CCTCGGCCAG CGGTCAGGGT CAGCAGCAAC AGCCGCCGAT ACCGCCGCCG 
CCGGCCAATG TGCCACCCCC GGAGCCGCCT AGAAGTCCGC CCGATTATGG CGGAGGAGGC 
GGCTTGGGCG TGGGAGCGAT GGGCGGCGCT GCCATGTCCC AGTACTCGGC CTCACCCTCG 
CCCACACAGC AGCAGCAGCA CCACCTCAAC CACCATCAGC AGCAGGCCAA CGGCTATGCT 
AATGGCACCG CCAACGGCTA TGGCTACATG CAGCAACAAG TGCAATCCAC GACAAACGCT 
TCACCACAGC ACGCTTCCAA CAACAACAGC AACAACCAGC AGCAGCAGCA ACAACAGCAG 
CAGCACCATC AGCAGCAGAT GCCGCAGCAC CACAACTCCG TTTTGAACGG TCACCCAAAC 
GGGCTAGCAG GTCCCTCCGC CCCACACAAC AACAACACCA CCCAAATGCC GTCCTCCCAA 
ATGAGGGGCC TGCTGAACGA AACAACAACC ACGCCGCCAC CAACAACAAC AACCCGAGCA 
CCACAAATCA CAACAACAGC AACAACAACA ACAACGGCGG CCACCACGGT AGCTATAAAG 
AGCGAGCAAA TGGAGGACAG CAACCACACT CACACCCACA CACACACGCA TCCCAATCAC 
AGTCACAGCC AGGACAGGAG CCACAGCAGC AGCAGCAGCA GCTCAATGGC CACGGAGGAG 
GCGGAGGAGC AGGAGCTGCG GGATCAGGAG CAGGCGGACG ATCACCTGCA TCAGCATCAA 
CAGGCGTCGC AGCAGTATCT GCTGTCGGCG CGTCATTACC ACAGCAGCAC GCCCAACACG 
CTCAGCAGCA GCAACACCAA TCCCAGCACG CCCAGCAGCA ACTCGCCCCA TACTATCTAC 
CGGCAGGAGC AGCAGGGAAC GGATTTCTCG CGCACCACCC CGCCGCCGCA GCCGCTGCCG 
CCTATGGGAA TGCTGCCGCC TATGGCAATG GACTACAACA TGCTGGCTTT GGATATGCCC 
ATGCCCATGC CCACGCTCAT GCACAGCAAT ATGCTGCAGT GCAGCAGCAC CAGCACCACG 
CCGCTAGCTA CTACCATCAC CACCAGTATG CCGGACACTA TGCAGCCGCC GCAGCAGCAG 
CTGGTGCACC ACTACCAGGC GGTGCTCCAC CCGCTCCATC AGCAGCTGGG GGAGCAGCAC 
CAGCGCCAGG AGGCGGATCA TCATCAGCAG CAGCGGGAGC TTCACCAACT GGATCAGCAG 
CAGCAGCAGC AGCAGGCGTT AATCCTGGCG GACAGTTTGC CGCACAGCAG CAGTTCGCCC 
ACCAGCAGCT CCCCACCGCC CACCATGCCC ATGCCGCTCA CCACCATCAC AGCGCCGCAG 
CTGCTGCCGC TGCAGCCGCC GCCGCCGCAC ATCACCAGCA CCATGCCCAT GCCGCCCACC 
ATGCACATGC CCATCATGCC GCCGCCGCCG CAATGCTACC AGCAGCTGCA GCCGCTGGAC 
CCCACAATGA GCTATCACAC TATTATTGGT AGTGGCCCGG AGGCGCATAC CGGAACGGCC 
GGCGGTGGCT ATAGCAATCA GATCACCACC AGCGATGGCC AGATCCTGCA GCTGATGCCA 
ACATCCCTGT TCGCGCCATA TGCACCGCTC TCGCCGTACT CGGTGGCCGC CCAGCGATCG 
CCGCAGGAGG GCGATCTGCC GCCGGTTCAC ACACTCACGA CGGCCCTGCA TGCCCATCAG 
CAGGGCGGGC AGCAGGAGGC GCAGACGCCA ACGCTGACTG TGCTCTCCAC GCCCTACTCG 
CCCACAGTGA GCAGCTCGCG GGCGACTCCC GCACTGGAGA TGGATATGGC CACGCTGATG 
CAGCACCATC AGGACTATGA GATGGAGCAG TACCAGATGC AGCACCAGCA GTTGGATCAG 
ATGCAGCAGC ACCAGCAGCA ACTGGACCAC CAGCAGCAGC AACAGATCCT GGCGGATCAG 
ACCCAGACGA TGGCCCAACA GCCGCTGGCC AAGAAGCGGC GTGGCGGCAA CGCAACACCA 
TCGACGACGA AGCGACGGCG GAACAGCAGC GTCGGATCGA CGTCGCCGCA CTCGACCACC 
CTGCCATCGG GACGGATCAA GTGTCTGGAG TGCGACAAGG AGTTCACCAA GAACTGCTAC 
CTCACGCAGC ACAACAAGAG CTTCCACTCC GGTGAGTACC CATTTCGCTG CCAGAAGTGC 
GGCAAGCGCT TTCAGAGCGA GGATGTCTAC ACCACGCACC TGGGTCGCCA CCGCACCCAG 
GACAAGCCGC ATAAGTGCGA GCTGTGCCCC AAGCAGTTCC ATCACAAGAC GGATCTGCGC 
CGACACGTGG AGGCCATCCA TACGGGGCTG AAGCAGCACA TGTGCGACAT CTGTGAGAAG 
GGCTTCTGTC GCAAGGATCA TCTGCGCAAG CACCTCGAGA CGCACAATCG GCCGCGGGTG 
GTGGGCAAGA AGTCCGCCGC GGCAGCAGCC GCCGCCGCAG CAGCAGCAGC AGTCACTGCC 
ACGGCGGGAT TGGGAGGTGC ACCAGCCGCC GGTTCCATTA AGGCGGCATT TGCCCGCTCG 
CTAACGGTGA CGGCTGTGAG CAGTGAGCCA GGCGCAGGAG TTGCAATACC CTCAGCTCCT 
GCCACTGCCA CATGTGCTCC TGTATCTGTA TCAGCGTCAG CTCTTGTCCA GGCCGTCAAT 
CCCAGTCTGA CTTCCGGCCT CAGTCTGAAG CGGCACATCG ACGATGTGGC CGTTGACGAT 
GACAGCCTGT TGGAGGAGGA CGAGGATGAG ATGGAGATGG AGATGGAGAT GGATATGTTG 
GAGGAGGAAG ATGAGGAGGA GGAGGAGCTG GGCGATCACC ACATGATAAT CAAGAGCGAG 
TACGTGCAGG AGGAGTTTCA GATGATCGAG AAGAGCATAG AGCTCTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

zld ( NM_134512.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_608356.1 Drosophila melanogaster zelda (zld), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa02182
Clone ID Related Accession (Same CDS sequence)	NM_167685.3 , NM_134512.5 , NM_001298537.1
Accession Version	NM_134512.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	zelda, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_134512.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGAGCA TTAAGACCGA GATGCCGCCC CTGCACGCGG CAGAGGCGCT CGCCTCCAGC 
AGTGCTACAG ACAGTGGTGG GGGAGGAGCA GGAGGAGGAG GAGGAGGAGG TGGAGGAGGA 
TCGGGCGGAC CAGGAGCGGG AGGAACGGGA GGCGTAGGCA GTGCGCCCGC CACACCCAAT 
GCCACAATTA GCGCCGCCGC CGACTCCAGT GATAATCAAC CCGGCACGCC GCAGCCCACG 
CAACAGCAGC AATCGACGCA GCAACAGTTG CAGCAGCCAC AATCGCAACA GCAGCAGCAG 
CAGGCCATGG GTGGAGGAGA TCCCCAGCAG CAGCAACAGC AGCAGCAGGT AACGGGTATC 
ACCCACCAGC CGTACGCCAC CCACCACATG TACTCCGCAT CTGGCGGACA GCAGCAACAA 
CAGCAGCAGA TCTACGGCGG ACTCTACGGC GGGGACATGC AGCAGCAGCA GGGTTACGCC 
AGCAGCTACA TCAACAGCTA CGAGCAGTTC TACCAGCAGC AACAGCAGCA GCAGCAGGGC 
ACTGATTACG CCTTTGGCAC CGTGGGCGTT GACTATGGCA AGAGCGCCGG TGTTCGCTAT 
CATCCCTACC TGCAGACGCC AACCTCCGGA CTGGGAGGCA TTCCAACGGC CAGCTCGGCA 
CAGGAGGAGG CGGGCAGTGC GCCCAGCGCG GTCAGCACCA CCACAGCGGT GGCCATGAGT 
CCGCGTGTGG TTAGCAGCAG CAGTCCAACG TCGACGTCCT CGCACCTGCA GCTGGGCAGC 
TCCAGTGGCC AGACACCAGG CTCACCAGGC GGAGCCGCCG GAAGTGCCGG ATGCAAGTTG 
CAGTGCAAGA AGTGCGGCAT CCTCACCACC AACGAGTCGG AGCTACAGGA GCACATCGCC 
AACGTGCACG GAGAGTCGCC GTACGGCAGC AGCGGTTACG CCAGTTCACC GTACATCAAG 
GAGGAGATGC CCACACCACA GCCACCCGGC GTTGGATCCG CAACGGCCAA TCCCGGCGAA 
TTACTCGACC TAGATTCCCA GAAGATGGTC TATCACCAGC AGCTGCTCCA GCAGCAACAA 
CAGCAACACG ACGTGGTCGC TGGACTGCCG CTGGGCGCTC TGCCGGATCC GCTGCACTCG 
ATGCAATCTA TGCAGCAGCG CGCACTCCAC AGCTGGGAGC AGCAGCCACA GCAGACGGTG 
GCCTCCGTGG AGGGCCTGCC ACCCTACATG CAGCAGGGAT TGGGCGTGGG GCTGGGAGTC 
GGTGTGGACA AGTCGCCCTA CTACTCACCA AAGCAGTCAC CCTATCATCA GTCAACCGGC 
GTTGGCGGAG CCACGCTGAT CAAGCAGGAG TACGGCGGCC ACGGACTGAT CAAGTCCGAG 
TATCCCGATT CGCAGCATTA CGTGGACAAG TCCTTCGATC CCACAGCTGG CGGAGCTGGT 
GGCGCGGAAC TGTGCGCCAG CGTGGCCACC AGTCCGGCGG AGTTCCCCAG CACCACTACG 
GGCGGACCAG GGCAGGAGGG AGCGGCGGGT GCGGCGCCAG GAGGTGGATA CCGCGGTTTC 
GAGCCACCCA GCTCGAGCTC CGTGCTGCCG GCCAACAGCC TGACGGCCAA GGCGGCCACC 
TGGAAGTCGA ACGAGGCGCG CCGTCCGAAA ACGTACAACT GCACGGCCTG CAACAAGTGG 
TTCACCAGCT CGGGCCATCT TAAGCGCCAC TACAATACGA CGCTGCACAA GAATGCGGTT 
AAGTCGAGCG GCCAGCCGGA TCCAGCCACC CTGCCCATCT CGGCGCACCA CCATCCCGCC 
CGCGACCCGG TGACGAAGCC CAGTCGCAGG GGCAATGCCG CAGCCGCTGC GGCCGCCGCT 
GCAGCTGCCG CCTCGGCCAG CGGTCAGGGT CAGCAGCAAC AGCCGCCGAT ACCGCCGCCG 
CCGGCCAATG TGCCACCCCC GGAGCCGCCT AGAAGTCCGC CCGATTATGG CGGAGGAGGC 
GGCTTGGGCG TGGGAGCGAT GGGCGGCGCT GCCATGTCCC AGTACTCGGC CTCACCCTCG 
CCCACACAGC AGCAGCAGCA CCACCTCAAC CACCATCAGC AGCAGGCCAA CGGCTATGCT 
AATGGCACCG CCAACGGCTA TGGCTACATG CAGCAACAAG TGCAATCCAC GACAAACGCT 
TCACCACAGC ACGCTTCCAA CAACAACAGC AACAACCAGC AGCAGCAGCA ACAACAGCAG 
CAGCACCATC AGCAGCAGAT GCCGCAGCAC CACAACTCCG TTTTGAACGG TCACCCAAAC 
GGGCTAGCAG GTCCCTCCGC CCCACACAAC AACAACACCA CCCAAATGCC GTCCTCCCAA 
ATGAGGGGCC TGCTGAACGA AACAACAACC ACGCCGCCAC CAACAACAAC AACCCGAGCA 
CCACAAATCA CAACAACAGC AACAACAACA ACAACGGCGG CCACCACGGT AGCTATAAAG 
AGCGAGCAAA TGGAGGACAG CAACCACACT CACACCCACA CACACACGCA TCCCAATCAC 
AGTCACAGCC AGGACAGGAG CCACAGCAGC AGCAGCAGCA GCTCAATGGC CACGGAGGAG 
GCGGAGGAGC AGGAGCTGCG GGATCAGGAG CAGGCGGACG ATCACCTGCA TCAGCATCAA 
CAGGCGTCGC AGCAGTATCT GCTGTCGGCG CGTCATTACC ACAGCAGCAC GCCCAACACG 
CTCAGCAGCA GCAACACCAA TCCCAGCACG CCCAGCAGCA ACTCGCCCCA TACTATCTAC 
CGGCAGGAGC AGCAGGGAAC GGATTTCTCG CGCACCACCC CGCCGCCGCA GCCGCTGCCG 
CCTATGGGAA TGCTGCCGCC TATGGCAATG GACTACAACA TGCTGGCTTT GGATATGCCC 
ATGCCCATGC CCACGCTCAT GCACAGCAAT ATGCTGCAGT GCAGCAGCAC CAGCACCACG 
CCGCTAGCTA CTACCATCAC CACCAGTATG CCGGACACTA TGCAGCCGCC GCAGCAGCAG 
CTGGTGCACC ACTACCAGGC GGTGCTCCAC CCGCTCCATC AGCAGCTGGG GGAGCAGCAC 
CAGCGCCAGG AGGCGGATCA TCATCAGCAG CAGCGGGAGC TTCACCAACT GGATCAGCAG 
CAGCAGCAGC AGCAGGCGTT AATCCTGGCG GACAGTTTGC CGCACAGCAG CAGTTCGCCC 
ACCAGCAGCT CCCCACCGCC CACCATGCCC ATGCCGCTCA CCACCATCAC AGCGCCGCAG 
CTGCTGCCGC TGCAGCCGCC GCCGCCGCAC ATCACCAGCA CCATGCCCAT GCCGCCCACC 
ATGCACATGC CCATCATGCC GCCGCCGCCG CAATGCTACC AGCAGCTGCA GCCGCTGGAC 
CCCACAATGA GCTATCACAC TATTATTGGT AGTGGCCCGG AGGCGCATAC CGGAACGGCC 
GGCGGTGGCT ATAGCAATCA GATCACCACC AGCGATGGCC AGATCCTGCA GCTGATGCCA 
ACATCCCTGT TCGCGCCATA TGCACCGCTC TCGCCGTACT CGGTGGCCGC CCAGCGATCG 
CCGCAGGAGG GCGATCTGCC GCCGGTTCAC ACACTCACGA CGGCCCTGCA TGCCCATCAG 
CAGGGCGGGC AGCAGGAGGC GCAGACGCCA ACGCTGACTG TGCTCTCCAC GCCCTACTCG 
CCCACAGTGA GCAGCTCGCG GGCGACTCCC GCACTGGAGA TGGATATGGC CACGCTGATG 
CAGCACCATC AGGACTATGA GATGGAGCAG TACCAGATGC AGCACCAGCA GTTGGATCAG 
ATGCAGCAGC ACCAGCAGCA ACTGGACCAC CAGCAGCAGC AACAGATCCT GGCGGATCAG 
ACCCAGACGA TGGCCCAACA GCCGCTGGCC AAGAAGCGGC GTGGCGGCAA CGCAACACCA 
TCGACGACGA AGCGACGGCG GAACAGCAGC GTCGGATCGA CGTCGCCGCA CTCGACCACC 
CTGCCATCGG GACGGATCAA GTGTCTGGAG TGCGACAAGG AGTTCACCAA GAACTGCTAC 
CTCACGCAGC ACAACAAGAG CTTCCACTCC GGTGAGTACC CATTTCGCTG CCAGAAGTGC 
GGCAAGCGCT TTCAGAGCGA GGATGTCTAC ACCACGCACC TGGGTCGCCA CCGCACCCAG 
GACAAGCCGC ATAAGTGCGA GCTGTGCCCC AAGCAGTTCC ATCACAAGAC GGATCTGCGC 
CGACACGTGG AGGCCATCCA TACGGGGCTG AAGCAGCACA TGTGCGACAT CTGTGAGAAG 
GGCTTCTGTC GCAAGGATCA TCTGCGCAAG CACCTCGAGA CGCACAATCG GCCGCGGGTG 
GTGGGCAAGA AGTCCGCCGC GGCAGCAGCC GCCGCCGCAG CAGCAGCAGC AGTCACTGCC 
ACGGCGGGAT TGGGAGGTGC ACCAGCCGCC GGTTCCATTA AGGCGGCATT TGCCCGCTCG 
CTAACGGTGA CGGCTGTGAG CAGTGAGCCA GGCGCAGGAG TTGCAATACC CTCAGCTCCT 
GCCACTGCCA CATGTGCTCC TGTATCTGTA TCAGCGTCAG CTCTTGTCCA GGCCGTCAAT 
CCCAGTCTGA CTTCCGGCCT CAGTCTGAAG CGGCACATCG ACGATGTGGC CGTTGACGAT 
GACAGCCTGT TGGAGGAGGA CGAGGATGAG ATGGAGATGG AGATGGAGAT GGATATGTTG 
GAGGAGGAAG ATGAGGAGGA GGAGGAGCTG GGCGATCACC ACATGATAAT CAAGAGCGAG 
TACGTGCAGG AGGAGTTTCA GATGATCGAG AAGAGCATAG AGCTCTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_608356.1
CDS	1263..6053
Translation	MTSIKTEMPPLHAAEALASSSATDSGGGGAGGGGGGGGGGSGGPGAGGTGGVGSAPATPNATISAAADSSDNQPGTPQPTQQQQSTQQQLQQPQSQQQQQQAMGGGDPQQQQQQQQVTGITHQPYATHHMYSASGGQQQQQQQIYGGLYGGDMQQQQGYASSYINSYEQFYQQQQQQQQGTDYAFGTVGVDYGKSAGVRYHPYLQTPTSGLGGIPTASSAQEEAGSAPSAVSTTTAVAMSPRVVSSSSPTSTSSHLQLGSSSGQTPGSPGGAAGSAGCKLQCKKCGILTTNESELQEHIANVHGESPYGSSGYASSPYIKEEMPTPQPPGVGSATANPGELLDLDSQKMVYHQQLLQQQQQQHDVVAGLPLGALPDPLHSMQSMQQRALHSWEQQPQQTVASVEGLPPYMQQGLGVGLGVGVDKSPYYSPKQSPYHQSTGVGGATLIKQEYGGHGLIKSEYPDSQHYVDKSFDPTAGGAGGAELCASVATSPAEFPSTTTGGPGQEGAAGAAPGGGYRGFEPPSSSSVLPANSLTAKAATWKSNEARRPKTYNCTACNKWFTSSGHLKRHYNTTLHKNAVKSSGQPDPATLPISAHHHPARDPVTKPSRRGNAAAAAAAAAAAASASGQGQQQQPPIPPPPANVPPPEPPRSPPDYGGGGGLGVGAMGGAAMSQYSASPSPTQQQQHHLNHHQQQANGYANGTANGYGYMQQQVQSTTNASPQHASNNNSNNQQQQQQQQQHHQQQMPQHHNSVLNGHPNGLAGPSAPHNNNTTQMPSSQMRGLLNETTTTPPPTTTTRAPQITTTATTTTTAATTVAIKSEQMEDSNHTHTHTHTHPNHSHSQDRSHSSSSSSSMATEEAEEQELRDQEQADDHLHQHQQASQQYLLSARHYHSSTPNTLSSSNTNPSTPSSNSPHTIYRQEQQGTDFSRTTPPPQPLPPMGMLPPMAMDYNMLALDMPMPMPTLMHSNMLQCSSTSTTPLATTITTSMPDTMQPPQQQLVHHYQAVLHPLHQQLGEQHQRQEADHHQQQRELHQLDQQQQQQQALILADSLPHSSSSPTSSSPPPTMPMPLTTITAPQLLPLQPPPPHITSTMPMPPTMHMPIMPPPPQCYQQLQPLDPTMSYHTIIGSGPEAHTGTAGGGYSNQITTSDGQILQLMPTSLFAPYAPLSPYSVAAQRSPQEGDLPPVHTLTTALHAHQQGGQQEAQTPTLTVLSTPYSPTVSSSRATPALEMDMATLMQHHQDYEMEQYQMQHQQLDQMQQHQQQLDHQQQQQILADQTQTMAQQPLAKKRRGGNATPSTTKRRRNSSVGSTSPHSTTLPSGRIKCLECDKEFTKNCYLTQHNKSFHSGEYPFRCQKCGKRFQSEDVYTTHLGRHRTQDKPHKCELCPKQFHHKTDLRRHVEAIHTGLKQHMCDICEKGFCRKDHLRKHLETHNRPRVVGKKSAAAAAAAAAAAAVTATAGLGGAPAAGSIKAAFARSLTVTAVSSEPGAGVAIPSAPATATCAPVSVSASALVQAVNPSLTSGLSLKRHIDDVAVDDDSLLEEDEDEMEMEMEMDMLEEEDEEEEELGDHHMIIKSEYVQEEFQMIEKSIELY

Target ORF information:

RefSeq Version	NM_134512.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster zelda (zld), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134512.5

ORF Insert Sequence:

ATGACGAGCA TTAAGACCGA GATGCCGCCC CTGCACGCGG CAGAGGCGCT CGCCTCCAGC 
AGTGCTACAG ACAGTGGTGG GGGAGGAGCA GGAGGAGGAG GAGGAGGAGG TGGAGGAGGA 
TCGGGCGGAC CAGGAGCGGG AGGAACGGGA GGCGTAGGCA GTGCGCCCGC CACACCCAAT 
GCCACAATTA GCGCCGCCGC CGACTCCAGT GATAATCAAC CCGGCACGCC GCAGCCCACG 
CAACAGCAGC AATCGACGCA GCAACAGTTG CAGCAGCCAC AATCGCAACA GCAGCAGCAG 
CAGGCCATGG GTGGAGGAGA TCCCCAGCAG CAGCAACAGC AGCAGCAGGT AACGGGTATC 
ACCCACCAGC CGTACGCCAC CCACCACATG TACTCCGCAT CTGGCGGACA GCAGCAACAA 
CAGCAGCAGA TCTACGGCGG ACTCTACGGC GGGGACATGC AGCAGCAGCA GGGTTACGCC 
AGCAGCTACA TCAACAGCTA CGAGCAGTTC TACCAGCAGC AACAGCAGCA GCAGCAGGGC 
ACTGATTACG CCTTTGGCAC CGTGGGCGTT GACTATGGCA AGAGCGCCGG TGTTCGCTAT 
CATCCCTACC TGCAGACGCC AACCTCCGGA CTGGGAGGCA TTCCAACGGC CAGCTCGGCA 
CAGGAGGAGG CGGGCAGTGC GCCCAGCGCG GTCAGCACCA CCACAGCGGT GGCCATGAGT 
CCGCGTGTGG TTAGCAGCAG CAGTCCAACG TCGACGTCCT CGCACCTGCA GCTGGGCAGC 
TCCAGTGGCC AGACACCAGG CTCACCAGGC GGAGCCGCCG GAAGTGCCGG ATGCAAGTTG 
CAGTGCAAGA AGTGCGGCAT CCTCACCACC AACGAGTCGG AGCTACAGGA GCACATCGCC 
AACGTGCACG GAGAGTCGCC GTACGGCAGC AGCGGTTACG CCAGTTCACC GTACATCAAG 
GAGGAGATGC CCACACCACA GCCACCCGGC GTTGGATCCG CAACGGCCAA TCCCGGCGAA 
TTACTCGACC TAGATTCCCA GAAGATGGTC TATCACCAGC AGCTGCTCCA GCAGCAACAA 
CAGCAACACG ACGTGGTCGC TGGACTGCCG CTGGGCGCTC TGCCGGATCC GCTGCACTCG 
ATGCAATCTA TGCAGCAGCG CGCACTCCAC AGCTGGGAGC AGCAGCCACA GCAGACGGTG 
GCCTCCGTGG AGGGCCTGCC ACCCTACATG CAGCAGGGAT TGGGCGTGGG GCTGGGAGTC 
GGTGTGGACA AGTCGCCCTA CTACTCACCA AAGCAGTCAC CCTATCATCA GTCAACCGGC 
GTTGGCGGAG CCACGCTGAT CAAGCAGGAG TACGGCGGCC ACGGACTGAT CAAGTCCGAG 
TATCCCGATT CGCAGCATTA CGTGGACAAG TCCTTCGATC CCACAGCTGG CGGAGCTGGT 
GGCGCGGAAC TGTGCGCCAG CGTGGCCACC AGTCCGGCGG AGTTCCCCAG CACCACTACG 
GGCGGACCAG GGCAGGAGGG AGCGGCGGGT GCGGCGCCAG GAGGTGGATA CCGCGGTTTC 
GAGCCACCCA GCTCGAGCTC CGTGCTGCCG GCCAACAGCC TGACGGCCAA GGCGGCCACC 
TGGAAGTCGA ACGAGGCGCG CCGTCCGAAA ACGTACAACT GCACGGCCTG CAACAAGTGG 
TTCACCAGCT CGGGCCATCT TAAGCGCCAC TACAATACGA CGCTGCACAA GAATGCGGTT 
AAGTCGAGCG GCCAGCCGGA TCCAGCCACC CTGCCCATCT CGGCGCACCA CCATCCCGCC 
CGCGACCCGG TGACGAAGCC CAGTCGCAGG GGCAATGCCG CAGCCGCTGC GGCCGCCGCT 
GCAGCTGCCG CCTCGGCCAG CGGTCAGGGT CAGCAGCAAC AGCCGCCGAT ACCGCCGCCG 
CCGGCCAATG TGCCACCCCC GGAGCCGCCT AGAAGTCCGC CCGATTATGG CGGAGGAGGC 
GGCTTGGGCG TGGGAGCGAT GGGCGGCGCT GCCATGTCCC AGTACTCGGC CTCACCCTCG 
CCCACACAGC AGCAGCAGCA CCACCTCAAC CACCATCAGC AGCAGGCCAA CGGCTATGCT 
AATGGCACCG CCAACGGCTA TGGCTACATG CAGCAACAAG TGCAATCCAC GACAAACGCT 
TCACCACAGC ACGCTTCCAA CAACAACAGC AACAACCAGC AGCAGCAGCA ACAACAGCAG 
CAGCACCATC AGCAGCAGAT GCCGCAGCAC CACAACTCCG TTTTGAACGG TCACCCAAAC 
GGGCTAGCAG GTCCCTCCGC CCCACACAAC AACAACACCA CCCAAATGCC GTCCTCCCAA 
ATGAGGGGCC TGCTGAACGA AACAACAACC ACGCCGCCAC CAACAACAAC AACCCGAGCA 
CCACAAATCA CAACAACAGC AACAACAACA ACAACGGCGG CCACCACGGT AGCTATAAAG 
AGCGAGCAAA TGGAGGACAG CAACCACACT CACACCCACA CACACACGCA TCCCAATCAC 
AGTCACAGCC AGGACAGGAG CCACAGCAGC AGCAGCAGCA GCTCAATGGC CACGGAGGAG 
GCGGAGGAGC AGGAGCTGCG GGATCAGGAG CAGGCGGACG ATCACCTGCA TCAGCATCAA 
CAGGCGTCGC AGCAGTATCT GCTGTCGGCG CGTCATTACC ACAGCAGCAC GCCCAACACG 
CTCAGCAGCA GCAACACCAA TCCCAGCACG CCCAGCAGCA ACTCGCCCCA TACTATCTAC 
CGGCAGGAGC AGCAGGGAAC GGATTTCTCG CGCACCACCC CGCCGCCGCA GCCGCTGCCG 
CCTATGGGAA TGCTGCCGCC TATGGCAATG GACTACAACA TGCTGGCTTT GGATATGCCC 
ATGCCCATGC CCACGCTCAT GCACAGCAAT ATGCTGCAGT GCAGCAGCAC CAGCACCACG 
CCGCTAGCTA CTACCATCAC CACCAGTATG CCGGACACTA TGCAGCCGCC GCAGCAGCAG 
CTGGTGCACC ACTACCAGGC GGTGCTCCAC CCGCTCCATC AGCAGCTGGG GGAGCAGCAC 
CAGCGCCAGG AGGCGGATCA TCATCAGCAG CAGCGGGAGC TTCACCAACT GGATCAGCAG 
CAGCAGCAGC AGCAGGCGTT AATCCTGGCG GACAGTTTGC CGCACAGCAG CAGTTCGCCC 
ACCAGCAGCT CCCCACCGCC CACCATGCCC ATGCCGCTCA CCACCATCAC AGCGCCGCAG 
CTGCTGCCGC TGCAGCCGCC GCCGCCGCAC ATCACCAGCA CCATGCCCAT GCCGCCCACC 
ATGCACATGC CCATCATGCC GCCGCCGCCG CAATGCTACC AGCAGCTGCA GCCGCTGGAC 
CCCACAATGA GCTATCACAC TATTATTGGT AGTGGCCCGG AGGCGCATAC CGGAACGGCC 
GGCGGTGGCT ATAGCAATCA GATCACCACC AGCGATGGCC AGATCCTGCA GCTGATGCCA 
ACATCCCTGT TCGCGCCATA TGCACCGCTC TCGCCGTACT CGGTGGCCGC CCAGCGATCG 
CCGCAGGAGG GCGATCTGCC GCCGGTTCAC ACACTCACGA CGGCCCTGCA TGCCCATCAG 
CAGGGCGGGC AGCAGGAGGC GCAGACGCCA ACGCTGACTG TGCTCTCCAC GCCCTACTCG 
CCCACAGTGA GCAGCTCGCG GGCGACTCCC GCACTGGAGA TGGATATGGC CACGCTGATG 
CAGCACCATC AGGACTATGA GATGGAGCAG TACCAGATGC AGCACCAGCA GTTGGATCAG 
ATGCAGCAGC ACCAGCAGCA ACTGGACCAC CAGCAGCAGC AACAGATCCT GGCGGATCAG 
ACCCAGACGA TGGCCCAACA GCCGCTGGCC AAGAAGCGGC GTGGCGGCAA CGCAACACCA 
TCGACGACGA AGCGACGGCG GAACAGCAGC GTCGGATCGA CGTCGCCGCA CTCGACCACC 
CTGCCATCGG GACGGATCAA GTGTCTGGAG TGCGACAAGG AGTTCACCAA GAACTGCTAC 
CTCACGCAGC ACAACAAGAG CTTCCACTCC GGTGAGTACC CATTTCGCTG CCAGAAGTGC 
GGCAAGCGCT TTCAGAGCGA GGATGTCTAC ACCACGCACC TGGGTCGCCA CCGCACCCAG 
GACAAGCCGC ATAAGTGCGA GCTGTGCCCC AAGCAGTTCC ATCACAAGAC GGATCTGCGC 
CGACACGTGG AGGCCATCCA TACGGGGCTG AAGCAGCACA TGTGCGACAT CTGTGAGAAG 
GGCTTCTGTC GCAAGGATCA TCTGCGCAAG CACCTCGAGA CGCACAATCG GCCGCGGGTG 
GTGGGCAAGA AGTCCGCCGC GGCAGCAGCC GCCGCCGCAG CAGCAGCAGC AGTCACTGCC 
ACGGCGGGAT TGGGAGGTGC ACCAGCCGCC GGTTCCATTA AGGCGGCATT TGCCCGCTCG 
CTAACGGTGA CGGCTGTGAG CAGTGAGCCA GGCGCAGGAG TTGCAATACC CTCAGCTCCT 
GCCACTGCCA CATGTGCTCC TGTATCTGTA TCAGCGTCAG CTCTTGTCCA GGCCGTCAAT 
CCCAGTCTGA CTTCCGGCCT CAGTCTGAAG CGGCACATCG ACGATGTGGC CGTTGACGAT 
GACAGCCTGT TGGAGGAGGA CGAGGATGAG ATGGAGATGG AGATGGAGAT GGATATGTTG 
GAGGAGGAAG ATGAGGAGGA GGAGGAGCTG GGCGATCACC ACATGATAAT CAAGAGCGAG 
TACGTGCAGG AGGAGTTTCA GATGATCGAG AAGAGCATAG AGCTCTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

zld ( NM_001298537.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285466.1 Drosophila melanogaster zelda (zld), transcript variant G, mRNA.

Price & Availability↑

CloneID	OFa02182
Clone ID Related Accession (Same CDS sequence)	NM_167685.3 , NM_134512.5 , NM_001298537.1
Accession Version	NM_001298537.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	zelda, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGAGCA TTAAGACCGA GATGCCGCCC CTGCACGCGG CAGAGGCGCT CGCCTCCAGC 
AGTGCTACAG ACAGTGGTGG GGGAGGAGCA GGAGGAGGAG GAGGAGGAGG TGGAGGAGGA 
TCGGGCGGAC CAGGAGCGGG AGGAACGGGA GGCGTAGGCA GTGCGCCCGC CACACCCAAT 
GCCACAATTA GCGCCGCCGC CGACTCCAGT GATAATCAAC CCGGCACGCC GCAGCCCACG 
CAACAGCAGC AATCGACGCA GCAACAGTTG CAGCAGCCAC AATCGCAACA GCAGCAGCAG 
CAGGCCATGG GTGGAGGAGA TCCCCAGCAG CAGCAACAGC AGCAGCAGGT AACGGGTATC 
ACCCACCAGC CGTACGCCAC CCACCACATG TACTCCGCAT CTGGCGGACA GCAGCAACAA 
CAGCAGCAGA TCTACGGCGG ACTCTACGGC GGGGACATGC AGCAGCAGCA GGGTTACGCC 
AGCAGCTACA TCAACAGCTA CGAGCAGTTC TACCAGCAGC AACAGCAGCA GCAGCAGGGC 
ACTGATTACG CCTTTGGCAC CGTGGGCGTT GACTATGGCA AGAGCGCCGG TGTTCGCTAT 
CATCCCTACC TGCAGACGCC AACCTCCGGA CTGGGAGGCA TTCCAACGGC CAGCTCGGCA 
CAGGAGGAGG CGGGCAGTGC GCCCAGCGCG GTCAGCACCA CCACAGCGGT GGCCATGAGT 
CCGCGTGTGG TTAGCAGCAG CAGTCCAACG TCGACGTCCT CGCACCTGCA GCTGGGCAGC 
TCCAGTGGCC AGACACCAGG CTCACCAGGC GGAGCCGCCG GAAGTGCCGG ATGCAAGTTG 
CAGTGCAAGA AGTGCGGCAT CCTCACCACC AACGAGTCGG AGCTACAGGA GCACATCGCC 
AACGTGCACG GAGAGTCGCC GTACGGCAGC AGCGGTTACG CCAGTTCACC GTACATCAAG 
GAGGAGATGC CCACACCACA GCCACCCGGC GTTGGATCCG CAACGGCCAA TCCCGGCGAA 
TTACTCGACC TAGATTCCCA GAAGATGGTC TATCACCAGC AGCTGCTCCA GCAGCAACAA 
CAGCAACACG ACGTGGTCGC TGGACTGCCG CTGGGCGCTC TGCCGGATCC GCTGCACTCG 
ATGCAATCTA TGCAGCAGCG CGCACTCCAC AGCTGGGAGC AGCAGCCACA GCAGACGGTG 
GCCTCCGTGG AGGGCCTGCC ACCCTACATG CAGCAGGGAT TGGGCGTGGG GCTGGGAGTC 
GGTGTGGACA AGTCGCCCTA CTACTCACCA AAGCAGTCAC CCTATCATCA GTCAACCGGC 
GTTGGCGGAG CCACGCTGAT CAAGCAGGAG TACGGCGGCC ACGGACTGAT CAAGTCCGAG 
TATCCCGATT CGCAGCATTA CGTGGACAAG TCCTTCGATC CCACAGCTGG CGGAGCTGGT 
GGCGCGGAAC TGTGCGCCAG CGTGGCCACC AGTCCGGCGG AGTTCCCCAG CACCACTACG 
GGCGGACCAG GGCAGGAGGG AGCGGCGGGT GCGGCGCCAG GAGGTGGATA CCGCGGTTTC 
GAGCCACCCA GCTCGAGCTC CGTGCTGCCG GCCAACAGCC TGACGGCCAA GGCGGCCACC 
TGGAAGTCGA ACGAGGCGCG CCGTCCGAAA ACGTACAACT GCACGGCCTG CAACAAGTGG 
TTCACCAGCT CGGGCCATCT TAAGCGCCAC TACAATACGA CGCTGCACAA GAATGCGGTT 
AAGTCGAGCG GCCAGCCGGA TCCAGCCACC CTGCCCATCT CGGCGCACCA CCATCCCGCC 
CGCGACCCGG TGACGAAGCC CAGTCGCAGG GGCAATGCCG CAGCCGCTGC GGCCGCCGCT 
GCAGCTGCCG CCTCGGCCAG CGGTCAGGGT CAGCAGCAAC AGCCGCCGAT ACCGCCGCCG 
CCGGCCAATG TGCCACCCCC GGAGCCGCCT AGAAGTCCGC CCGATTATGG CGGAGGAGGC 
GGCTTGGGCG TGGGAGCGAT GGGCGGCGCT GCCATGTCCC AGTACTCGGC CTCACCCTCG 
CCCACACAGC AGCAGCAGCA CCACCTCAAC CACCATCAGC AGCAGGCCAA CGGCTATGCT 
AATGGCACCG CCAACGGCTA TGGCTACATG CAGCAACAAG TGCAATCCAC GACAAACGCT 
TCACCACAGC ACGCTTCCAA CAACAACAGC AACAACCAGC AGCAGCAGCA ACAACAGCAG 
CAGCACCATC AGCAGCAGAT GCCGCAGCAC CACAACTCCG TTTTGAACGG TCACCCAAAC 
GGGCTAGCAG GTCCCTCCGC CCCACACAAC AACAACACCA CCCAAATGCC GTCCTCCCAA 
ATGAGGGGCC TGCTGAACGA AACAACAACC ACGCCGCCAC CAACAACAAC AACCCGAGCA 
CCACAAATCA CAACAACAGC AACAACAACA ACAACGGCGG CCACCACGGT AGCTATAAAG 
AGCGAGCAAA TGGAGGACAG CAACCACACT CACACCCACA CACACACGCA TCCCAATCAC 
AGTCACAGCC AGGACAGGAG CCACAGCAGC AGCAGCAGCA GCTCAATGGC CACGGAGGAG 
GCGGAGGAGC AGGAGCTGCG GGATCAGGAG CAGGCGGACG ATCACCTGCA TCAGCATCAA 
CAGGCGTCGC AGCAGTATCT GCTGTCGGCG CGTCATTACC ACAGCAGCAC GCCCAACACG 
CTCAGCAGCA GCAACACCAA TCCCAGCACG CCCAGCAGCA ACTCGCCCCA TACTATCTAC 
CGGCAGGAGC AGCAGGGAAC GGATTTCTCG CGCACCACCC CGCCGCCGCA GCCGCTGCCG 
CCTATGGGAA TGCTGCCGCC TATGGCAATG GACTACAACA TGCTGGCTTT GGATATGCCC 
ATGCCCATGC CCACGCTCAT GCACAGCAAT ATGCTGCAGT GCAGCAGCAC CAGCACCACG 
CCGCTAGCTA CTACCATCAC CACCAGTATG CCGGACACTA TGCAGCCGCC GCAGCAGCAG 
CTGGTGCACC ACTACCAGGC GGTGCTCCAC CCGCTCCATC AGCAGCTGGG GGAGCAGCAC 
CAGCGCCAGG AGGCGGATCA TCATCAGCAG CAGCGGGAGC TTCACCAACT GGATCAGCAG 
CAGCAGCAGC AGCAGGCGTT AATCCTGGCG GACAGTTTGC CGCACAGCAG CAGTTCGCCC 
ACCAGCAGCT CCCCACCGCC CACCATGCCC ATGCCGCTCA CCACCATCAC AGCGCCGCAG 
CTGCTGCCGC TGCAGCCGCC GCCGCCGCAC ATCACCAGCA CCATGCCCAT GCCGCCCACC 
ATGCACATGC CCATCATGCC GCCGCCGCCG CAATGCTACC AGCAGCTGCA GCCGCTGGAC 
CCCACAATGA GCTATCACAC TATTATTGGT AGTGGCCCGG AGGCGCATAC CGGAACGGCC 
GGCGGTGGCT ATAGCAATCA GATCACCACC AGCGATGGCC AGATCCTGCA GCTGATGCCA 
ACATCCCTGT TCGCGCCATA TGCACCGCTC TCGCCGTACT CGGTGGCCGC CCAGCGATCG 
CCGCAGGAGG GCGATCTGCC GCCGGTTCAC ACACTCACGA CGGCCCTGCA TGCCCATCAG 
CAGGGCGGGC AGCAGGAGGC GCAGACGCCA ACGCTGACTG TGCTCTCCAC GCCCTACTCG 
CCCACAGTGA GCAGCTCGCG GGCGACTCCC GCACTGGAGA TGGATATGGC CACGCTGATG 
CAGCACCATC AGGACTATGA GATGGAGCAG TACCAGATGC AGCACCAGCA GTTGGATCAG 
ATGCAGCAGC ACCAGCAGCA ACTGGACCAC CAGCAGCAGC AACAGATCCT GGCGGATCAG 
ACCCAGACGA TGGCCCAACA GCCGCTGGCC AAGAAGCGGC GTGGCGGCAA CGCAACACCA 
TCGACGACGA AGCGACGGCG GAACAGCAGC GTCGGATCGA CGTCGCCGCA CTCGACCACC 
CTGCCATCGG GACGGATCAA GTGTCTGGAG TGCGACAAGG AGTTCACCAA GAACTGCTAC 
CTCACGCAGC ACAACAAGAG CTTCCACTCC GGTGAGTACC CATTTCGCTG CCAGAAGTGC 
GGCAAGCGCT TTCAGAGCGA GGATGTCTAC ACCACGCACC TGGGTCGCCA CCGCACCCAG 
GACAAGCCGC ATAAGTGCGA GCTGTGCCCC AAGCAGTTCC ATCACAAGAC GGATCTGCGC 
CGACACGTGG AGGCCATCCA TACGGGGCTG AAGCAGCACA TGTGCGACAT CTGTGAGAAG 
GGCTTCTGTC GCAAGGATCA TCTGCGCAAG CACCTCGAGA CGCACAATCG GCCGCGGGTG 
GTGGGCAAGA AGTCCGCCGC GGCAGCAGCC GCCGCCGCAG CAGCAGCAGC AGTCACTGCC 
ACGGCGGGAT TGGGAGGTGC ACCAGCCGCC GGTTCCATTA AGGCGGCATT TGCCCGCTCG 
CTAACGGTGA CGGCTGTGAG CAGTGAGCCA GGCGCAGGAG TTGCAATACC CTCAGCTCCT 
GCCACTGCCA CATGTGCTCC TGTATCTGTA TCAGCGTCAG CTCTTGTCCA GGCCGTCAAT 
CCCAGTCTGA CTTCCGGCCT CAGTCTGAAG CGGCACATCG ACGATGTGGC CGTTGACGAT 
GACAGCCTGT TGGAGGAGGA CGAGGATGAG ATGGAGATGG AGATGGAGAT GGATATGTTG 
GAGGAGGAAG ATGAGGAGGA GGAGGAGCTG GGCGATCACC ACATGATAAT CAAGAGCGAG 
TACGTGCAGG AGGAGTTTCA GATGATCGAG AAGAGCATAG AGCTCTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285466.1
CDS	858..5648
Translation	MTSIKTEMPPLHAAEALASSSATDSGGGGAGGGGGGGGGGSGGPGAGGTGGVGSAPATPNATISAAADSSDNQPGTPQPTQQQQSTQQQLQQPQSQQQQQQAMGGGDPQQQQQQQQVTGITHQPYATHHMYSASGGQQQQQQQIYGGLYGGDMQQQQGYASSYINSYEQFYQQQQQQQQGTDYAFGTVGVDYGKSAGVRYHPYLQTPTSGLGGIPTASSAQEEAGSAPSAVSTTTAVAMSPRVVSSSSPTSTSSHLQLGSSSGQTPGSPGGAAGSAGCKLQCKKCGILTTNESELQEHIANVHGESPYGSSGYASSPYIKEEMPTPQPPGVGSATANPGELLDLDSQKMVYHQQLLQQQQQQHDVVAGLPLGALPDPLHSMQSMQQRALHSWEQQPQQTVASVEGLPPYMQQGLGVGLGVGVDKSPYYSPKQSPYHQSTGVGGATLIKQEYGGHGLIKSEYPDSQHYVDKSFDPTAGGAGGAELCASVATSPAEFPSTTTGGPGQEGAAGAAPGGGYRGFEPPSSSSVLPANSLTAKAATWKSNEARRPKTYNCTACNKWFTSSGHLKRHYNTTLHKNAVKSSGQPDPATLPISAHHHPARDPVTKPSRRGNAAAAAAAAAAAASASGQGQQQQPPIPPPPANVPPPEPPRSPPDYGGGGGLGVGAMGGAAMSQYSASPSPTQQQQHHLNHHQQQANGYANGTANGYGYMQQQVQSTTNASPQHASNNNSNNQQQQQQQQQHHQQQMPQHHNSVLNGHPNGLAGPSAPHNNNTTQMPSSQMRGLLNETTTTPPPTTTTRAPQITTTATTTTTAATTVAIKSEQMEDSNHTHTHTHTHPNHSHSQDRSHSSSSSSSMATEEAEEQELRDQEQADDHLHQHQQASQQYLLSARHYHSSTPNTLSSSNTNPSTPSSNSPHTIYRQEQQGTDFSRTTPPPQPLPPMGMLPPMAMDYNMLALDMPMPMPTLMHSNMLQCSSTSTTPLATTITTSMPDTMQPPQQQLVHHYQAVLHPLHQQLGEQHQRQEADHHQQQRELHQLDQQQQQQQALILADSLPHSSSSPTSSSPPPTMPMPLTTITAPQLLPLQPPPPHITSTMPMPPTMHMPIMPPPPQCYQQLQPLDPTMSYHTIIGSGPEAHTGTAGGGYSNQITTSDGQILQLMPTSLFAPYAPLSPYSVAAQRSPQEGDLPPVHTLTTALHAHQQGGQQEAQTPTLTVLSTPYSPTVSSSRATPALEMDMATLMQHHQDYEMEQYQMQHQQLDQMQQHQQQLDHQQQQQILADQTQTMAQQPLAKKRRGGNATPSTTKRRRNSSVGSTSPHSTTLPSGRIKCLECDKEFTKNCYLTQHNKSFHSGEYPFRCQKCGKRFQSEDVYTTHLGRHRTQDKPHKCELCPKQFHHKTDLRRHVEAIHTGLKQHMCDICEKGFCRKDHLRKHLETHNRPRVVGKKSAAAAAAAAAAAAVTATAGLGGAPAAGSIKAAFARSLTVTAVSSEPGAGVAIPSAPATATCAPVSVSASALVQAVNPSLTSGLSLKRHIDDVAVDDDSLLEEDEDEMEMEMEMDMLEEEDEEEEELGDHHMIIKSEYVQEEFQMIEKSIELY

Target ORF information:

RefSeq Version	NM_001298537.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster zelda (zld), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298537.1

ORF Insert Sequence:

ATGACGAGCA TTAAGACCGA GATGCCGCCC CTGCACGCGG CAGAGGCGCT CGCCTCCAGC 
AGTGCTACAG ACAGTGGTGG GGGAGGAGCA GGAGGAGGAG GAGGAGGAGG TGGAGGAGGA 
TCGGGCGGAC CAGGAGCGGG AGGAACGGGA GGCGTAGGCA GTGCGCCCGC CACACCCAAT 
GCCACAATTA GCGCCGCCGC CGACTCCAGT GATAATCAAC CCGGCACGCC GCAGCCCACG 
CAACAGCAGC AATCGACGCA GCAACAGTTG CAGCAGCCAC AATCGCAACA GCAGCAGCAG 
CAGGCCATGG GTGGAGGAGA TCCCCAGCAG CAGCAACAGC AGCAGCAGGT AACGGGTATC 
ACCCACCAGC CGTACGCCAC CCACCACATG TACTCCGCAT CTGGCGGACA GCAGCAACAA 
CAGCAGCAGA TCTACGGCGG ACTCTACGGC GGGGACATGC AGCAGCAGCA GGGTTACGCC 
AGCAGCTACA TCAACAGCTA CGAGCAGTTC TACCAGCAGC AACAGCAGCA GCAGCAGGGC 
ACTGATTACG CCTTTGGCAC CGTGGGCGTT GACTATGGCA AGAGCGCCGG TGTTCGCTAT 
CATCCCTACC TGCAGACGCC AACCTCCGGA CTGGGAGGCA TTCCAACGGC CAGCTCGGCA 
CAGGAGGAGG CGGGCAGTGC GCCCAGCGCG GTCAGCACCA CCACAGCGGT GGCCATGAGT 
CCGCGTGTGG TTAGCAGCAG CAGTCCAACG TCGACGTCCT CGCACCTGCA GCTGGGCAGC 
TCCAGTGGCC AGACACCAGG CTCACCAGGC GGAGCCGCCG GAAGTGCCGG ATGCAAGTTG 
CAGTGCAAGA AGTGCGGCAT CCTCACCACC AACGAGTCGG AGCTACAGGA GCACATCGCC 
AACGTGCACG GAGAGTCGCC GTACGGCAGC AGCGGTTACG CCAGTTCACC GTACATCAAG 
GAGGAGATGC CCACACCACA GCCACCCGGC GTTGGATCCG CAACGGCCAA TCCCGGCGAA 
TTACTCGACC TAGATTCCCA GAAGATGGTC TATCACCAGC AGCTGCTCCA GCAGCAACAA 
CAGCAACACG ACGTGGTCGC TGGACTGCCG CTGGGCGCTC TGCCGGATCC GCTGCACTCG 
ATGCAATCTA TGCAGCAGCG CGCACTCCAC AGCTGGGAGC AGCAGCCACA GCAGACGGTG 
GCCTCCGTGG AGGGCCTGCC ACCCTACATG CAGCAGGGAT TGGGCGTGGG GCTGGGAGTC 
GGTGTGGACA AGTCGCCCTA CTACTCACCA AAGCAGTCAC CCTATCATCA GTCAACCGGC 
GTTGGCGGAG CCACGCTGAT CAAGCAGGAG TACGGCGGCC ACGGACTGAT CAAGTCCGAG 
TATCCCGATT CGCAGCATTA CGTGGACAAG TCCTTCGATC CCACAGCTGG CGGAGCTGGT 
GGCGCGGAAC TGTGCGCCAG CGTGGCCACC AGTCCGGCGG AGTTCCCCAG CACCACTACG 
GGCGGACCAG GGCAGGAGGG AGCGGCGGGT GCGGCGCCAG GAGGTGGATA CCGCGGTTTC 
GAGCCACCCA GCTCGAGCTC CGTGCTGCCG GCCAACAGCC TGACGGCCAA GGCGGCCACC 
TGGAAGTCGA ACGAGGCGCG CCGTCCGAAA ACGTACAACT GCACGGCCTG CAACAAGTGG 
TTCACCAGCT CGGGCCATCT TAAGCGCCAC TACAATACGA CGCTGCACAA GAATGCGGTT 
AAGTCGAGCG GCCAGCCGGA TCCAGCCACC CTGCCCATCT CGGCGCACCA CCATCCCGCC 
CGCGACCCGG TGACGAAGCC CAGTCGCAGG GGCAATGCCG CAGCCGCTGC GGCCGCCGCT 
GCAGCTGCCG CCTCGGCCAG CGGTCAGGGT CAGCAGCAAC AGCCGCCGAT ACCGCCGCCG 
CCGGCCAATG TGCCACCCCC GGAGCCGCCT AGAAGTCCGC CCGATTATGG CGGAGGAGGC 
GGCTTGGGCG TGGGAGCGAT GGGCGGCGCT GCCATGTCCC AGTACTCGGC CTCACCCTCG 
CCCACACAGC AGCAGCAGCA CCACCTCAAC CACCATCAGC AGCAGGCCAA CGGCTATGCT 
AATGGCACCG CCAACGGCTA TGGCTACATG CAGCAACAAG TGCAATCCAC GACAAACGCT 
TCACCACAGC ACGCTTCCAA CAACAACAGC AACAACCAGC AGCAGCAGCA ACAACAGCAG 
CAGCACCATC AGCAGCAGAT GCCGCAGCAC CACAACTCCG TTTTGAACGG TCACCCAAAC 
GGGCTAGCAG GTCCCTCCGC CCCACACAAC AACAACACCA CCCAAATGCC GTCCTCCCAA 
ATGAGGGGCC TGCTGAACGA AACAACAACC ACGCCGCCAC CAACAACAAC AACCCGAGCA 
CCACAAATCA CAACAACAGC AACAACAACA ACAACGGCGG CCACCACGGT AGCTATAAAG 
AGCGAGCAAA TGGAGGACAG CAACCACACT CACACCCACA CACACACGCA TCCCAATCAC 
AGTCACAGCC AGGACAGGAG CCACAGCAGC AGCAGCAGCA GCTCAATGGC CACGGAGGAG 
GCGGAGGAGC AGGAGCTGCG GGATCAGGAG CAGGCGGACG ATCACCTGCA TCAGCATCAA 
CAGGCGTCGC AGCAGTATCT GCTGTCGGCG CGTCATTACC ACAGCAGCAC GCCCAACACG 
CTCAGCAGCA GCAACACCAA TCCCAGCACG CCCAGCAGCA ACTCGCCCCA TACTATCTAC 
CGGCAGGAGC AGCAGGGAAC GGATTTCTCG CGCACCACCC CGCCGCCGCA GCCGCTGCCG 
CCTATGGGAA TGCTGCCGCC TATGGCAATG GACTACAACA TGCTGGCTTT GGATATGCCC 
ATGCCCATGC CCACGCTCAT GCACAGCAAT ATGCTGCAGT GCAGCAGCAC CAGCACCACG 
CCGCTAGCTA CTACCATCAC CACCAGTATG CCGGACACTA TGCAGCCGCC GCAGCAGCAG 
CTGGTGCACC ACTACCAGGC GGTGCTCCAC CCGCTCCATC AGCAGCTGGG GGAGCAGCAC 
CAGCGCCAGG AGGCGGATCA TCATCAGCAG CAGCGGGAGC TTCACCAACT GGATCAGCAG 
CAGCAGCAGC AGCAGGCGTT AATCCTGGCG GACAGTTTGC CGCACAGCAG CAGTTCGCCC 
ACCAGCAGCT CCCCACCGCC CACCATGCCC ATGCCGCTCA CCACCATCAC AGCGCCGCAG 
CTGCTGCCGC TGCAGCCGCC GCCGCCGCAC ATCACCAGCA CCATGCCCAT GCCGCCCACC 
ATGCACATGC CCATCATGCC GCCGCCGCCG CAATGCTACC AGCAGCTGCA GCCGCTGGAC 
CCCACAATGA GCTATCACAC TATTATTGGT AGTGGCCCGG AGGCGCATAC CGGAACGGCC 
GGCGGTGGCT ATAGCAATCA GATCACCACC AGCGATGGCC AGATCCTGCA GCTGATGCCA 
ACATCCCTGT TCGCGCCATA TGCACCGCTC TCGCCGTACT CGGTGGCCGC CCAGCGATCG 
CCGCAGGAGG GCGATCTGCC GCCGGTTCAC ACACTCACGA CGGCCCTGCA TGCCCATCAG 
CAGGGCGGGC AGCAGGAGGC GCAGACGCCA ACGCTGACTG TGCTCTCCAC GCCCTACTCG 
CCCACAGTGA GCAGCTCGCG GGCGACTCCC GCACTGGAGA TGGATATGGC CACGCTGATG 
CAGCACCATC AGGACTATGA GATGGAGCAG TACCAGATGC AGCACCAGCA GTTGGATCAG 
ATGCAGCAGC ACCAGCAGCA ACTGGACCAC CAGCAGCAGC AACAGATCCT GGCGGATCAG 
ACCCAGACGA TGGCCCAACA GCCGCTGGCC AAGAAGCGGC GTGGCGGCAA CGCAACACCA 
TCGACGACGA AGCGACGGCG GAACAGCAGC GTCGGATCGA CGTCGCCGCA CTCGACCACC 
CTGCCATCGG GACGGATCAA GTGTCTGGAG TGCGACAAGG AGTTCACCAA GAACTGCTAC 
CTCACGCAGC ACAACAAGAG CTTCCACTCC GGTGAGTACC CATTTCGCTG CCAGAAGTGC 
GGCAAGCGCT TTCAGAGCGA GGATGTCTAC ACCACGCACC TGGGTCGCCA CCGCACCCAG 
GACAAGCCGC ATAAGTGCGA GCTGTGCCCC AAGCAGTTCC ATCACAAGAC GGATCTGCGC 
CGACACGTGG AGGCCATCCA TACGGGGCTG AAGCAGCACA TGTGCGACAT CTGTGAGAAG 
GGCTTCTGTC GCAAGGATCA TCTGCGCAAG CACCTCGAGA CGCACAATCG GCCGCGGGTG 
GTGGGCAAGA AGTCCGCCGC GGCAGCAGCC GCCGCCGCAG CAGCAGCAGC AGTCACTGCC 
ACGGCGGGAT TGGGAGGTGC ACCAGCCGCC GGTTCCATTA AGGCGGCATT TGCCCGCTCG 
CTAACGGTGA CGGCTGTGAG CAGTGAGCCA GGCGCAGGAG TTGCAATACC CTCAGCTCCT 
GCCACTGCCA CATGTGCTCC TGTATCTGTA TCAGCGTCAG CTCTTGTCCA GGCCGTCAAT 
CCCAGTCTGA CTTCCGGCCT CAGTCTGAAG CGGCACATCG ACGATGTGGC CGTTGACGAT 
GACAGCCTGT TGGAGGAGGA CGAGGATGAG ATGGAGATGG AGATGGAGAT GGATATGTTG 
GAGGAGGAAG ATGAGGAGGA GGAGGAGCTG GGCGATCACC ACATGATAAT CAAGAGCGAG 
TACGTGCAGG AGGAGTTTCA GATGATCGAG AAGAGCATAG AGCTCTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

zld ( NM_001258840.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245769.1 Drosophila melanogaster zelda (zld), transcript variant D, mRNA.

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CloneID	OFa03176
Clone ID Related Accession (Same CDS sequence)	NM_001258840.3
Accession Version	NM_001258840.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	zelda, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001258840.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGAGCA TTAAGACCGA GATGCCGCCC CTGCACGCGG CAGAGGCGCT CGCCTCCAGC 
AGTGCTACAG ACAGTGGTGG GGGAGGAGCA GGAGGAGGAG GAGGAGGAGG TGGAGGAGGA 
TCGGGCGGAC CAGGAGCGGG AGGAACGGGA GGCGTAGGCA GTGCGCCCGC CACACCCAAT 
GCCACAATTA GCGCCGCCGC CGACTCCAGT GATAATCAAC CCGGCACGCC GCAGCCCACG 
CAACAGCAGC AATCGACGCA GCAACAGTTG CAGCAGCCAC AATCGCAACA GCAGCAGCAG 
CAGGCCATGG GTGGAGGAGA TCCCCAGCAG CAGCAACAGC AGCAGCAGGT AACGGGTATC 
ACCCACCAGC CGTACGCCAC CCACCACATG TACTCCGCAT CTGGCGGACA GCAGCAACAA 
CAGCAGCAGA TCTACGGCGG ACTCTACGGC GGGGACATGC AGCAGCAGCA GGGTTACGCC 
AGCAGCTACA TCAACAGCTA CGAGCAGTTC TACCAGCAGC AACAGCAGCA GCAGCAGGGC 
ACTGATTACG CCTTTGGCAC CGTGGGCGTT GACTATGGCA AGAGCGCCGG TGTTCGCTAT 
CATCCCTACC TGCAGACGCC AACCTCCGGA CTGGGAGGCA TTCCAACGGC CAGCTCGGCA 
CAGGAGGAGG CGGGCAGTGC GCCCAGCGCG GTCAGCACCA CCACAGCGGT GGCCATGAGT 
CCGCGTGTGG TTAGCAGCAG CAGTCCAACG TCGACGTCCT CGCACCTGCA GCTGGGCAGC 
TCCAGTGGCC AGACACCAGG CTCACCAGGC GGAGCCGCCG GAAGTGCCGG ATGCAAGTTG 
CAGTGCAAGA AGTGCGGCAT CCTCACCACC AACGAGTCGG AGCTACAGGA GCACATCGCC 
AACGTGCACG GAGAGTCGCC GTACGGCAGC AGCGGTTACG CCAGTTCACC GTACATCAAG 
GAGGAGATGC CCACACCACA GCCACCCGGC GTTGGATCCG CAACGGCCAA TCCCGGCGAA 
TTACTCGACC TAGATTCCCA GAAGATGGTC TATCACCAGC AGCTGCTCCA GCAGCAACAA 
CAGCAACACG ACGTGGTCGC TGGACTGCCG CTGGGCGCTC TGCCGGATCC GCTGCACTCG 
ATGCAATCTA TGCAGCAGCG CGCACTCCAC AGCTGGGAGC AGCAGCCACA GCAGACGGTG 
GCCTCCGTGG AGGGCCTGCC ACCCTACATG CAGCAGGGAT TGGGCGTGGG GCTGGGAGTC 
GGTGTGGACA AGTCGCCCTA CTACTCACCA AAGCAGTCAC CCTATCATCA GTCAACCGGC 
GTTGGCGGAG CCACGCTGAT CAAGCAGGAG TACGGCGGCC ACGGACTGAT CAAGTCCGAG 
TATCCCGATT CGCAGCATTA CGTGGACAAG TCCTTCGATC CCACAGCTGG CGGAGCTGGT 
GGCGCGGAAC TGTGCGCCAG CGTGGCCACC AGTCCGGCGG AGTTCCCCAG CACCACTACG 
GGCGGACCAG GGCAGGAGGG AGCGGCGGGT GCGGCGCCAG GAGGTGGATA CCGCGGTTTC 
GAGCCACCCA GCTCGAGCTC CGTGCTGCCG GCCAACAGCC TGACGGCCAA GGCGGCCACC 
TGGAAGTCGA ACGAGGCGCG CCGTCCGAAA ACGTACAACT GCACGGCCTG CAACAAGTGG 
TTCACCAGCT CGGGCCATCT TAAGCGCCAC TACAATACGA CGCTGCACAA GAATGCGGTT 
AAGTCGAGCG GCCAGCCGGA TCCAGCCACC CTGCCCATCT CGGCGCACCA CCATCCCGCC 
CGCGACCCGG TGACGAAGCC CAGTCGCAGG GGCAATGCCG CAGCCGCTGC GGCCGCCGCT 
GCAGCTGCCG CCTCGGCCAG CGGTCAGGGT CAGCAGCAAC AGCCGCCGAT ACCGCCGCCG 
CCGGCCAATG TGCCACCCCC GGAGCCGCCT AGAAGTCCGC CCGATTATGG CGGAGGAGGC 
GGCTTGGGCG TGGGAGCGAT GGGCGGCGCT GCCATGTCCC AGTACTCGGC CTCACCCTCG 
CCCACACAGC AGCAGCAGCA CCACCTCAAC CACCATCAGC AGCAGGCCAA CGGCTATGCT 
AATGGCACCG CCAACGGCTA TGGCTACATG CAGCAACAAG TGCAATCCAC GACAAACGCT 
TCACCACAGC ACGCTTCCAA CAACAACAGC AACAACCAGC AGCAGCAGCA ACAACAGCAG 
CAGCACCATC AGCAGCAGAT GCCGCAGCAC CACAACTCCG TTTTGAACGG TCACCCAAAC 
GGGCTAGCAG GTCCCTCCGC CCCACACAAC AACAACACCA CCCAAATGCC GTCCTCCCAA 
ATGAGGGGCC TGCTGAACGA AACAACAACC ACGCCGCCAC CAACAACAAC AACCCGAGCA 
CCACAAATCA CAACAACAGC AACAACAACA ACAACGGCGG CCACCACGGT AGCTATAAAG 
AGCGAGCAAA TGGAGGACAG CAACCACACT CACACCCACA CACACACGCA TCCCAATCAC 
AGTCACAGCC AGGACAGGAG CCACAGCAGC AGCAGCAGCA GCTCAATGGC CACGGAGGAG 
GCGGAGGAGC AGGAGCTGCG GGATCAGGAG CAGGCGGACG ATCACCTGCA TCAGCATCAA 
CAGGCGTCGC AGCAGTATCT GCTGTCGGCG CGTCATTACC ACAGCAGCAC GCCCAACACG 
CTCAGCAGCA GCAACACCAA TCCCAGCACG CCCAGCAGCA ACTCGCCCCA TACTATCTAC 
CGGCAGGAGC AGCAGGGAAC GGATTTCTCG CGCACCACCC CGCCGCCGCA GCCGCTGCCG 
CCTATGGGAA TGCTGCCGCC TATGGCAATG GACTACAACA TGCTGGCTTT GGATATGCCC 
ATGCCCATGC CCACGCTCAT GCACAGCAAT ATGCTGCAGT GCAGCAGCAC CAGCACCACG 
CCGCTAGCTA CTACCATCAC CACCAGTATG CCGGACACTA TGCAGCCGCC GCAGCAGCAG 
CTGGTGCACC ACTACCAGGC GGTGCTCCAC CCGCTCCATC AGCAGCTGGG GGAGCAGCAC 
CAGCGCCAGG AGGCGGATCA TCATCAGCAG CAGCGGGAGC TTCACCAACT GGATCAGCAG 
CAGCAGCAGC AGCAGGCGTT AATCCTGGCG GACAGTTTGC CGCACAGCAG CAGTTCGCCC 
ACCAGCAGCT CCCCACCGCC CACCATGCCC ATGCCGCTCA CCACCATCAC AGCGCCGCAG 
CTGCTGCCGC TGCAGCCGCC GCCGCCGCAC ATCACCAGCA CCATGCCCAT GCCGCCCACC 
ATGCACATGC CCATCATGCC GCCGCCGCCG CAATGCTACC AGCAGCTGCA GCCGCTGGAC 
CCCACAATGA GCTATCACAC TATTATTGGT AGTGGCCCGG AGGCGCATAC CGGAACGGCC 
GGCGGTGGCT ATAGCAATCA GATCACCACC AGCGATGGCC AGATCCTGCA GCTGATGCCA 
ACATCCCTGT TCGCGCCATA TGCACCGCTC TCGCCGTACT CGGTGGCCGC CCAGCGATCG 
CCGCAGGAGG GCGATCTGCC GCCGGTTCAC ACACTCACGA CGGCCCTGCA TGCCCATCAG 
CAGGGCGGGC AGCAGGAGGC GCAGACGCCA ACGCTGACTG TGCTCTCCAC GCCCTACTCG 
CCCACAGTGA GCAGCTCGCG GGCGACTCCC GCACTGGAGA TGGATATGGC CACGCTGATG 
CAGCACCATC AGGACTATGA GATGGAGCAG TACCAGATGC AGCACCAGCA GTTGGATCAG 
ATGCAGCAGC ACCAGCAGCA ACTGGACCAC CAGCAGCAGC AACAGATCCT GGCGGATCAG 
ACCCAGACGA TGGCCCAACA GCCGCTGGCC AAGAAGCGGC GTGGCGGCAA CGCAACACCA 
TCGACGACGA AGCGACGGCG GAACAGCAGC GTCGGATCGA CGTCGCCGCA CTCGACCACC 
CTGCCATCGG GACGGATCAA GTGTCTGGAG TGCGACAAGG AGTTCACCAA GAACTGCTAC 
CTCACGCAGC ACAACAAGAG CTTCCACTCC GCATCCAGGA GGCAGAACAA ATCGAATCCA 
TCTATGTACC CAGCGAACGC CCAGAGACGG ATCAAAATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245769.1
CDS	918..5039
Translation	MTSIKTEMPPLHAAEALASSSATDSGGGGAGGGGGGGGGGSGGPGAGGTGGVGSAPATPNATISAAADSSDNQPGTPQPTQQQQSTQQQLQQPQSQQQQQQAMGGGDPQQQQQQQQVTGITHQPYATHHMYSASGGQQQQQQQIYGGLYGGDMQQQQGYASSYINSYEQFYQQQQQQQQGTDYAFGTVGVDYGKSAGVRYHPYLQTPTSGLGGIPTASSAQEEAGSAPSAVSTTTAVAMSPRVVSSSSPTSTSSHLQLGSSSGQTPGSPGGAAGSAGCKLQCKKCGILTTNESELQEHIANVHGESPYGSSGYASSPYIKEEMPTPQPPGVGSATANPGELLDLDSQKMVYHQQLLQQQQQQHDVVAGLPLGALPDPLHSMQSMQQRALHSWEQQPQQTVASVEGLPPYMQQGLGVGLGVGVDKSPYYSPKQSPYHQSTGVGGATLIKQEYGGHGLIKSEYPDSQHYVDKSFDPTAGGAGGAELCASVATSPAEFPSTTTGGPGQEGAAGAAPGGGYRGFEPPSSSSVLPANSLTAKAATWKSNEARRPKTYNCTACNKWFTSSGHLKRHYNTTLHKNAVKSSGQPDPATLPISAHHHPARDPVTKPSRRGNAAAAAAAAAAAASASGQGQQQQPPIPPPPANVPPPEPPRSPPDYGGGGGLGVGAMGGAAMSQYSASPSPTQQQQHHLNHHQQQANGYANGTANGYGYMQQQVQSTTNASPQHASNNNSNNQQQQQQQQQHHQQQMPQHHNSVLNGHPNGLAGPSAPHNNNTTQMPSSQMRGLLNETTTTPPPTTTTRAPQITTTATTTTTAATTVAIKSEQMEDSNHTHTHTHTHPNHSHSQDRSHSSSSSSSMATEEAEEQELRDQEQADDHLHQHQQASQQYLLSARHYHSSTPNTLSSSNTNPSTPSSNSPHTIYRQEQQGTDFSRTTPPPQPLPPMGMLPPMAMDYNMLALDMPMPMPTLMHSNMLQCSSTSTTPLATTITTSMPDTMQPPQQQLVHHYQAVLHPLHQQLGEQHQRQEADHHQQQRELHQLDQQQQQQQALILADSLPHSSSSPTSSSPPPTMPMPLTTITAPQLLPLQPPPPHITSTMPMPPTMHMPIMPPPPQCYQQLQPLDPTMSYHTIIGSGPEAHTGTAGGGYSNQITTSDGQILQLMPTSLFAPYAPLSPYSVAAQRSPQEGDLPPVHTLTTALHAHQQGGQQEAQTPTLTVLSTPYSPTVSSSRATPALEMDMATLMQHHQDYEMEQYQMQHQQLDQMQQHQQQLDHQQQQQILADQTQTMAQQPLAKKRRGGNATPSTTKRRRNSSVGSTSPHSTTLPSGRIKCLECDKEFTKNCYLTQHNKSFHSASRRQNKSNPSMYPANAQRRIKI

Target ORF information:

RefSeq Version	NM_001258840.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster zelda (zld), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258840.3

ORF Insert Sequence:

ATGACGAGCA TTAAGACCGA GATGCCGCCC CTGCACGCGG CAGAGGCGCT CGCCTCCAGC 
AGTGCTACAG ACAGTGGTGG GGGAGGAGCA GGAGGAGGAG GAGGAGGAGG TGGAGGAGGA 
TCGGGCGGAC CAGGAGCGGG AGGAACGGGA GGCGTAGGCA GTGCGCCCGC CACACCCAAT 
GCCACAATTA GCGCCGCCGC CGACTCCAGT GATAATCAAC CCGGCACGCC GCAGCCCACG 
CAACAGCAGC AATCGACGCA GCAACAGTTG CAGCAGCCAC AATCGCAACA GCAGCAGCAG 
CAGGCCATGG GTGGAGGAGA TCCCCAGCAG CAGCAACAGC AGCAGCAGGT AACGGGTATC 
ACCCACCAGC CGTACGCCAC CCACCACATG TACTCCGCAT CTGGCGGACA GCAGCAACAA 
CAGCAGCAGA TCTACGGCGG ACTCTACGGC GGGGACATGC AGCAGCAGCA GGGTTACGCC 
AGCAGCTACA TCAACAGCTA CGAGCAGTTC TACCAGCAGC AACAGCAGCA GCAGCAGGGC 
ACTGATTACG CCTTTGGCAC CGTGGGCGTT GACTATGGCA AGAGCGCCGG TGTTCGCTAT 
CATCCCTACC TGCAGACGCC AACCTCCGGA CTGGGAGGCA TTCCAACGGC CAGCTCGGCA 
CAGGAGGAGG CGGGCAGTGC GCCCAGCGCG GTCAGCACCA CCACAGCGGT GGCCATGAGT 
CCGCGTGTGG TTAGCAGCAG CAGTCCAACG TCGACGTCCT CGCACCTGCA GCTGGGCAGC 
TCCAGTGGCC AGACACCAGG CTCACCAGGC GGAGCCGCCG GAAGTGCCGG ATGCAAGTTG 
CAGTGCAAGA AGTGCGGCAT CCTCACCACC AACGAGTCGG AGCTACAGGA GCACATCGCC 
AACGTGCACG GAGAGTCGCC GTACGGCAGC AGCGGTTACG CCAGTTCACC GTACATCAAG 
GAGGAGATGC CCACACCACA GCCACCCGGC GTTGGATCCG CAACGGCCAA TCCCGGCGAA 
TTACTCGACC TAGATTCCCA GAAGATGGTC TATCACCAGC AGCTGCTCCA GCAGCAACAA 
CAGCAACACG ACGTGGTCGC TGGACTGCCG CTGGGCGCTC TGCCGGATCC GCTGCACTCG 
ATGCAATCTA TGCAGCAGCG CGCACTCCAC AGCTGGGAGC AGCAGCCACA GCAGACGGTG 
GCCTCCGTGG AGGGCCTGCC ACCCTACATG CAGCAGGGAT TGGGCGTGGG GCTGGGAGTC 
GGTGTGGACA AGTCGCCCTA CTACTCACCA AAGCAGTCAC CCTATCATCA GTCAACCGGC 
GTTGGCGGAG CCACGCTGAT CAAGCAGGAG TACGGCGGCC ACGGACTGAT CAAGTCCGAG 
TATCCCGATT CGCAGCATTA CGTGGACAAG TCCTTCGATC CCACAGCTGG CGGAGCTGGT 
GGCGCGGAAC TGTGCGCCAG CGTGGCCACC AGTCCGGCGG AGTTCCCCAG CACCACTACG 
GGCGGACCAG GGCAGGAGGG AGCGGCGGGT GCGGCGCCAG GAGGTGGATA CCGCGGTTTC 
GAGCCACCCA GCTCGAGCTC CGTGCTGCCG GCCAACAGCC TGACGGCCAA GGCGGCCACC 
TGGAAGTCGA ACGAGGCGCG CCGTCCGAAA ACGTACAACT GCACGGCCTG CAACAAGTGG 
TTCACCAGCT CGGGCCATCT TAAGCGCCAC TACAATACGA CGCTGCACAA GAATGCGGTT 
AAGTCGAGCG GCCAGCCGGA TCCAGCCACC CTGCCCATCT CGGCGCACCA CCATCCCGCC 
CGCGACCCGG TGACGAAGCC CAGTCGCAGG GGCAATGCCG CAGCCGCTGC GGCCGCCGCT 
GCAGCTGCCG CCTCGGCCAG CGGTCAGGGT CAGCAGCAAC AGCCGCCGAT ACCGCCGCCG 
CCGGCCAATG TGCCACCCCC GGAGCCGCCT AGAAGTCCGC CCGATTATGG CGGAGGAGGC 
GGCTTGGGCG TGGGAGCGAT GGGCGGCGCT GCCATGTCCC AGTACTCGGC CTCACCCTCG 
CCCACACAGC AGCAGCAGCA CCACCTCAAC CACCATCAGC AGCAGGCCAA CGGCTATGCT 
AATGGCACCG CCAACGGCTA TGGCTACATG CAGCAACAAG TGCAATCCAC GACAAACGCT 
TCACCACAGC ACGCTTCCAA CAACAACAGC AACAACCAGC AGCAGCAGCA ACAACAGCAG 
CAGCACCATC AGCAGCAGAT GCCGCAGCAC CACAACTCCG TTTTGAACGG TCACCCAAAC 
GGGCTAGCAG GTCCCTCCGC CCCACACAAC AACAACACCA CCCAAATGCC GTCCTCCCAA 
ATGAGGGGCC TGCTGAACGA AACAACAACC ACGCCGCCAC CAACAACAAC AACCCGAGCA 
CCACAAATCA CAACAACAGC AACAACAACA ACAACGGCGG CCACCACGGT AGCTATAAAG 
AGCGAGCAAA TGGAGGACAG CAACCACACT CACACCCACA CACACACGCA TCCCAATCAC 
AGTCACAGCC AGGACAGGAG CCACAGCAGC AGCAGCAGCA GCTCAATGGC CACGGAGGAG 
GCGGAGGAGC AGGAGCTGCG GGATCAGGAG CAGGCGGACG ATCACCTGCA TCAGCATCAA 
CAGGCGTCGC AGCAGTATCT GCTGTCGGCG CGTCATTACC ACAGCAGCAC GCCCAACACG 
CTCAGCAGCA GCAACACCAA TCCCAGCACG CCCAGCAGCA ACTCGCCCCA TACTATCTAC 
CGGCAGGAGC AGCAGGGAAC GGATTTCTCG CGCACCACCC CGCCGCCGCA GCCGCTGCCG 
CCTATGGGAA TGCTGCCGCC TATGGCAATG GACTACAACA TGCTGGCTTT GGATATGCCC 
ATGCCCATGC CCACGCTCAT GCACAGCAAT ATGCTGCAGT GCAGCAGCAC CAGCACCACG 
CCGCTAGCTA CTACCATCAC CACCAGTATG CCGGACACTA TGCAGCCGCC GCAGCAGCAG 
CTGGTGCACC ACTACCAGGC GGTGCTCCAC CCGCTCCATC AGCAGCTGGG GGAGCAGCAC 
CAGCGCCAGG AGGCGGATCA TCATCAGCAG CAGCGGGAGC TTCACCAACT GGATCAGCAG 
CAGCAGCAGC AGCAGGCGTT AATCCTGGCG GACAGTTTGC CGCACAGCAG CAGTTCGCCC 
ACCAGCAGCT CCCCACCGCC CACCATGCCC ATGCCGCTCA CCACCATCAC AGCGCCGCAG 
CTGCTGCCGC TGCAGCCGCC GCCGCCGCAC ATCACCAGCA CCATGCCCAT GCCGCCCACC 
ATGCACATGC CCATCATGCC GCCGCCGCCG CAATGCTACC AGCAGCTGCA GCCGCTGGAC 
CCCACAATGA GCTATCACAC TATTATTGGT AGTGGCCCGG AGGCGCATAC CGGAACGGCC 
GGCGGTGGCT ATAGCAATCA GATCACCACC AGCGATGGCC AGATCCTGCA GCTGATGCCA 
ACATCCCTGT TCGCGCCATA TGCACCGCTC TCGCCGTACT CGGTGGCCGC CCAGCGATCG 
CCGCAGGAGG GCGATCTGCC GCCGGTTCAC ACACTCACGA CGGCCCTGCA TGCCCATCAG 
CAGGGCGGGC AGCAGGAGGC GCAGACGCCA ACGCTGACTG TGCTCTCCAC GCCCTACTCG 
CCCACAGTGA GCAGCTCGCG GGCGACTCCC GCACTGGAGA TGGATATGGC CACGCTGATG 
CAGCACCATC AGGACTATGA GATGGAGCAG TACCAGATGC AGCACCAGCA GTTGGATCAG 
ATGCAGCAGC ACCAGCAGCA ACTGGACCAC CAGCAGCAGC AACAGATCCT GGCGGATCAG 
ACCCAGACGA TGGCCCAACA GCCGCTGGCC AAGAAGCGGC GTGGCGGCAA CGCAACACCA 
TCGACGACGA AGCGACGGCG GAACAGCAGC GTCGGATCGA CGTCGCCGCA CTCGACCACC 
CTGCCATCGG GACGGATCAA GTGTCTGGAG TGCGACAAGG AGTTCACCAA GAACTGCTAC 
CTCACGCAGC ACAACAAGAG CTTCCACTCC GCATCCAGGA GGCAGAACAA ATCGAATCCA 
TCTATGTACC CAGCGAACGC CCAGAGACGG ATCAAAATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

zld ( NM_001258839.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245768.1 Drosophila melanogaster zelda (zld), transcript variant F, mRNA.

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CloneID	OFa21549
Clone ID Related Accession (Same CDS sequence)	NM_001258839.2
Accession Version	NM_001258839.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	zelda, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001258839.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGAGCA TTAAGACCGA GATGCCGCCC CTGCACGCGG CAGAGGCGCT CGCCTCCAGC 
AGTGCTACAG ACAGTGGTGG GGGAGGAGCA GGAGGAGGAG GAGGAGGAGG TGGAGGAGGA 
TCGGGCGGAC CAGGAGCGGG AGGAACGGGA GGCGTAGGCA GTGCGCCCGC CACACCCAAT 
GCCACAATTA GCGCCGCCGC CGACTCCAGT GATAATCAAC CCGGCACGCC GCAGCCCACG 
CAACAGCAGC AATCGACGCA GCAACAGTTG CAGCAGCCAC AATCGCAACA GCAGCAGCAG 
CAGGCCATGG GTGGAGGAGA TCCCCAGCAG CAGCAACAGC AGCAGCAGGT AACGGGTATC 
ACCCACCAGC CGTACGCCAC CCACCACATG TACTCCGCAT CTGGCGGACA GCAGCAACAA 
CAGCAGCAGA TCTACGGCGG ACTCTACGGC GGGGACATGC AGCAGCAGCA GGGTTACGCC 
AGCAGCTACA TCAACAGCTA CGAGCAGTTC TACCAGCAGC AACAGCAGCA GCAGCAGGGC 
ACTGATTACG CCTTTGGCAC CGTGGGCGTT GACTATGGCA AGAGCGCCGG TGTTCGCTAT 
CATCCCTACC TGCAGACGCC AACCTCCGGA CTGGGAGGCA TTCCAACGGC CAGCTCGGCA 
CAGGAGGAGG CGGGCAGTGC GCCCAGCGCG GTCAGCACCA CCACAGCGGT GGCCATGAGT 
CCGCGTGTGG TTAGCAGCAG CAGTCCAACG TCGACGTCCT CGCACCTGCA GCTGGGCAGC 
TCCAGTGGCC AGACACCAGG CTCACCAGGC GGAGCCGCCG GAAGTGCCGG ATGCAAGTTG 
CAGTGCAAGA AGTGCGGCAT CCTCACCACC AACGAGTCGG AGCTACAGGA GCACATCGCC 
AACGTGCACG GAGAGTCGCC GTACGGCAGC AGCGGTTACG CCAGTTCACC GTACATCAAG 
GAGGAGATGC CCACACCACA GCCACCCGGC GTTGGATCCG CAACGGCCAA TCCCGGCGAA 
TTACTCGACC TAGATTCCCA GAAGATGGTC TATCACCAGC AGCTGCTCCA GCAGCAACAA 
CAGCAACACG ACGTGGTCGC TGGACTGCCG CTGGGCGCTC TGCCGGATCC GCTGCACTCG 
ATGCAATCTA TGCAGCAGCG CGCACTCCAC AGCTGGGAGC AGCAGCCACA GCAGACGGTG 
GCCTCCGTGG AGGGCCTGCC ACCCTACATG CAGCAGGGAT TGGGCGTGGG GCTGGGAGTC 
GGTGTGGACA AGTCGCCCTA CTACTCACCA AAGCAGTCAC CCTATCATCA GTCAACCGGC 
GTTGGCGGAG CCACGCTGAT CAAGCAGGAG TACGGCGGCC ACGGACTGAT CAAGTCCGAG 
TATCCCGATT CGCAGCATTA CGTGGACAAG TCCTTCGATC CCACAGCTGG CGGAGCTGGT 
GGCGCGGAAC TGTGCGCCAG CGTGGCCACC AGTCCGGCGG AGTTCCCCAG CACCACTACG 
GGCGGACCAG GGCAGGAGGG AGCGGCGGGT GCGGCGCCAG GAGGTGGATA CCGCGGTTTC 
GAGCCACCCA GCTCGAGCTC CGTGCTGCCG GCCAACAGCC TGACGGCCAA GGCGGCCACC 
TGGAAGTCGA ACGAGGCGCG CCGTCCGAAA ACGTACAACT GCACGGCCTG CAACAAGTGG 
TTCACCAGCT CGGGCCATCT TAAGCGCCAC TACAATACGA CGCTGCACAA GAATGCGGTT 
AAGTCGAGCG GCCAGCCGGA TCCAGCCACC CTGCCCATCT CGGCGCACCA CCATCCCGCC 
CGCGACCCGG TGACGAAGCC CAGTCGCAGG GGCAATGCCG CAGCCGCTGC GGCCGCCGCT 
GCAGCTGCCG CCTCGGCCAG CGGTCAGGGT CAGCAGCAAC AGCCGCCGAT ACCGCCGCCG 
CCGGCCAATG TGCCACCCCC GGAGCCGCCT AGAAGTCCGC CCGATTATGG CGGAGGAGGC 
GGCTTGGGCG TGGGAGCGAT GGGCGGCGCT GCCATGTCCC AGTACTCGGC CTCACCCTCG 
CCCACACAGC AGCAGCAGCA CCACCTCAAC CACCATCAGC AGCAGGCCAA CGGCTATGCT 
AATGGCACCG CCAACGGCTA TGGCTACATG CAGCAACAAG TGCAATCCAC GACAAACGCT 
TCACCACAGC ACGCTTCCAA CAACAACAGC AACAACCAGC AGCAGCAGCA ACAACAGCAG 
CAGCACCATC AGCAGCAGAT GCCGCAGCAC CACAACTCCG TTTTGAACGG TCACCCAAAC 
GGGCTAGCAG GTCCCTCCGC CCCACACAAC AACAACACCA CCCAAATGCC GTCCTCCCAA 
ATGAGGGGCC TGCTGAACGA AACAACAACC ACGCCGCCAC CAACAACAAC AACCCGAGCA 
CCACAAATCA CAACAACAGC AACAACAACA ACAACGGCGG CCACCACGGT AGCTATAAAG 
AGCGAGCAAA TGGAGGACAG CAACCACACT CACACCCACA CACACACGCA TCCCAATCAC 
AGTCACAGCC AGGACAGGAG CCACAGCAGC AGCAGCAGCA GCTCAATGGC CACGGAGGAG 
GCGGAGGAGC AGGAGCTGCG GGATCAGGAG CAGGCGGACG ATCACCTGCA TCAGCATCAA 
CAGGCGTCGC AGCAGTATCT GCTGTCGGCG CGTCATTACC ACAGCAGCAC GCCCAACACG 
CTCAGCAGCA GCAACACCAA TCCCAGCACG CCCAGCAGCA ACTCGCCCCA TACTATCTAC 
CGGCAGGAGC AGCAGGGAAC GGATTTCTCG CGCACCACCC CGCCGCCGCA GCCGCTGCCG 
CCTATGGGAA TGCTGCCGCC TATGGCAATG GACTACAACA TGCTGGCTTT GGATATGCCC 
ATGCCCATGC CCACGCTCAT GCACAGCAAT ATGCTGCAGT GCAGCAGCAC CAGCACCACG 
CCGCTAGCTA CTACCATCAC CACCAGTATG CCGGACACTA TGCAGCCGCC GCAGCAGCAG 
CTGGTGCACC ACTACCAGGC GGTGCTCCAC CCGCTCCATC AGCAGCTGGG GGAGCAGCAC 
CAGCGCCAGG AGGCGGATCA TCATCAGCAG CAGCGGGAGC TTCACCAACT GGATCAGCAG 
CAGCAGCAGC AGCAGGCGTT AATCCTGGCG GACAGTTTGC CGCACAGCAG CAGTTCGCCC 
ACCAGCAGCT CCCCACCGCC CACCATGCCC ATGCCGCTCA CCACCATCAC AGCGCCGCAG 
CTGCTGCCGC TGCAGCCGCC GCCGCCGCAC ATCACCAGCA CCATGCCCAT GCCGCCCACC 
ATGCACATGC CCATCATGCC GCCGCCGCCG CAATGCTACC AGCAGCTGCA GCCGCTGGAC 
CCCACAATGA GCTATCACAC TATTATTGGT AGTGGCCCGG AGGCGCATAC CGGAACGGCC 
GGCGGTGGCT ATAGCAATCA GATCACCACC AGCGATGGCC AGATCCTGCA GCTGATGCCA 
ACATCCCTGT TCGCGCCATA TGCACCGCTC TCGCCGTACT CGGTGGCCGC CCAGCGATCG 
CCGCAGGAGG GCGATCTGCC GCCGGTTCAC ACACTCACGA CGGCCCTGCA TGCCCATCAG 
CAGGGCGGGC AGCAGGAGGC GCAGACGCCA ACGCTGACTG TGCTCTCCAC GCCCTACTCG 
CCCACAGTGA GCAGCTCGCG GGCGACTCCC GCACTGGAGA TGGATATGGC CACGCTGATG 
CAGCACCATC AGGACTATGA GATGGAGCAG TACCAGATGC AGCACCAGCA GTTGGATCAG 
ATGCAGCAGC ACCAGCAGCA ACTGGACCAC CAGCAGCAGC AACAGATCCT GGCGGATCAG 
ACCCAGACGA TGGCCCAACA GCCGCTGGCC AAGAAGCGGC GTGGCGGCAA CGCAACACCA 
TCGACGACGA AGCGACGGCG GAACAGCAGC GTCGGATCGA CGTCGCCGCA CTCGACCACC 
CTGCCATCGG GACGGATCAA GTGTCTGGAG TGCGACAAGG AGTTCACCAA GAACTGCTAC 
CTCACGCAGC ACAACAAGAG CTTCCACTCC GTGAGTTTGC CATATGTATG TGTGAAACGA 
TTGAAAGACA GCCAGAATTC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245768.1
CDS	918..5021
Translation	MTSIKTEMPPLHAAEALASSSATDSGGGGAGGGGGGGGGGSGGPGAGGTGGVGSAPATPNATISAAADSSDNQPGTPQPTQQQQSTQQQLQQPQSQQQQQQAMGGGDPQQQQQQQQVTGITHQPYATHHMYSASGGQQQQQQQIYGGLYGGDMQQQQGYASSYINSYEQFYQQQQQQQQGTDYAFGTVGVDYGKSAGVRYHPYLQTPTSGLGGIPTASSAQEEAGSAPSAVSTTTAVAMSPRVVSSSSPTSTSSHLQLGSSSGQTPGSPGGAAGSAGCKLQCKKCGILTTNESELQEHIANVHGESPYGSSGYASSPYIKEEMPTPQPPGVGSATANPGELLDLDSQKMVYHQQLLQQQQQQHDVVAGLPLGALPDPLHSMQSMQQRALHSWEQQPQQTVASVEGLPPYMQQGLGVGLGVGVDKSPYYSPKQSPYHQSTGVGGATLIKQEYGGHGLIKSEYPDSQHYVDKSFDPTAGGAGGAELCASVATSPAEFPSTTTGGPGQEGAAGAAPGGGYRGFEPPSSSSVLPANSLTAKAATWKSNEARRPKTYNCTACNKWFTSSGHLKRHYNTTLHKNAVKSSGQPDPATLPISAHHHPARDPVTKPSRRGNAAAAAAAAAAAASASGQGQQQQPPIPPPPANVPPPEPPRSPPDYGGGGGLGVGAMGGAAMSQYSASPSPTQQQQHHLNHHQQQANGYANGTANGYGYMQQQVQSTTNASPQHASNNNSNNQQQQQQQQQHHQQQMPQHHNSVLNGHPNGLAGPSAPHNNNTTQMPSSQMRGLLNETTTTPPPTTTTRAPQITTTATTTTTAATTVAIKSEQMEDSNHTHTHTHTHPNHSHSQDRSHSSSSSSSMATEEAEEQELRDQEQADDHLHQHQQASQQYLLSARHYHSSTPNTLSSSNTNPSTPSSNSPHTIYRQEQQGTDFSRTTPPPQPLPPMGMLPPMAMDYNMLALDMPMPMPTLMHSNMLQCSSTSTTPLATTITTSMPDTMQPPQQQLVHHYQAVLHPLHQQLGEQHQRQEADHHQQQRELHQLDQQQQQQQALILADSLPHSSSSPTSSSPPPTMPMPLTTITAPQLLPLQPPPPHITSTMPMPPTMHMPIMPPPPQCYQQLQPLDPTMSYHTIIGSGPEAHTGTAGGGYSNQITTSDGQILQLMPTSLFAPYAPLSPYSVAAQRSPQEGDLPPVHTLTTALHAHQQGGQQEAQTPTLTVLSTPYSPTVSSSRATPALEMDMATLMQHHQDYEMEQYQMQHQQLDQMQQHQQQLDHQQQQQILADQTQTMAQQPLAKKRRGGNATPSTTKRRRNSSVGSTSPHSTTLPSGRIKCLECDKEFTKNCYLTQHNKSFHSVSLPYVCVKRLKDSQNS

Target ORF information:

RefSeq Version	NM_001258839.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster zelda (zld), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258839.2

ORF Insert Sequence:

ATGACGAGCA TTAAGACCGA GATGCCGCCC CTGCACGCGG CAGAGGCGCT CGCCTCCAGC 
AGTGCTACAG ACAGTGGTGG GGGAGGAGCA GGAGGAGGAG GAGGAGGAGG TGGAGGAGGA 
TCGGGCGGAC CAGGAGCGGG AGGAACGGGA GGCGTAGGCA GTGCGCCCGC CACACCCAAT 
GCCACAATTA GCGCCGCCGC CGACTCCAGT GATAATCAAC CCGGCACGCC GCAGCCCACG 
CAACAGCAGC AATCGACGCA GCAACAGTTG CAGCAGCCAC AATCGCAACA GCAGCAGCAG 
CAGGCCATGG GTGGAGGAGA TCCCCAGCAG CAGCAACAGC AGCAGCAGGT AACGGGTATC 
ACCCACCAGC CGTACGCCAC CCACCACATG TACTCCGCAT CTGGCGGACA GCAGCAACAA 
CAGCAGCAGA TCTACGGCGG ACTCTACGGC GGGGACATGC AGCAGCAGCA GGGTTACGCC 
AGCAGCTACA TCAACAGCTA CGAGCAGTTC TACCAGCAGC AACAGCAGCA GCAGCAGGGC 
ACTGATTACG CCTTTGGCAC CGTGGGCGTT GACTATGGCA AGAGCGCCGG TGTTCGCTAT 
CATCCCTACC TGCAGACGCC AACCTCCGGA CTGGGAGGCA TTCCAACGGC CAGCTCGGCA 
CAGGAGGAGG CGGGCAGTGC GCCCAGCGCG GTCAGCACCA CCACAGCGGT GGCCATGAGT 
CCGCGTGTGG TTAGCAGCAG CAGTCCAACG TCGACGTCCT CGCACCTGCA GCTGGGCAGC 
TCCAGTGGCC AGACACCAGG CTCACCAGGC GGAGCCGCCG GAAGTGCCGG ATGCAAGTTG 
CAGTGCAAGA AGTGCGGCAT CCTCACCACC AACGAGTCGG AGCTACAGGA GCACATCGCC 
AACGTGCACG GAGAGTCGCC GTACGGCAGC AGCGGTTACG CCAGTTCACC GTACATCAAG 
GAGGAGATGC CCACACCACA GCCACCCGGC GTTGGATCCG CAACGGCCAA TCCCGGCGAA 
TTACTCGACC TAGATTCCCA GAAGATGGTC TATCACCAGC AGCTGCTCCA GCAGCAACAA 
CAGCAACACG ACGTGGTCGC TGGACTGCCG CTGGGCGCTC TGCCGGATCC GCTGCACTCG 
ATGCAATCTA TGCAGCAGCG CGCACTCCAC AGCTGGGAGC AGCAGCCACA GCAGACGGTG 
GCCTCCGTGG AGGGCCTGCC ACCCTACATG CAGCAGGGAT TGGGCGTGGG GCTGGGAGTC 
GGTGTGGACA AGTCGCCCTA CTACTCACCA AAGCAGTCAC CCTATCATCA GTCAACCGGC 
GTTGGCGGAG CCACGCTGAT CAAGCAGGAG TACGGCGGCC ACGGACTGAT CAAGTCCGAG 
TATCCCGATT CGCAGCATTA CGTGGACAAG TCCTTCGATC CCACAGCTGG CGGAGCTGGT 
GGCGCGGAAC TGTGCGCCAG CGTGGCCACC AGTCCGGCGG AGTTCCCCAG CACCACTACG 
GGCGGACCAG GGCAGGAGGG AGCGGCGGGT GCGGCGCCAG GAGGTGGATA CCGCGGTTTC 
GAGCCACCCA GCTCGAGCTC CGTGCTGCCG GCCAACAGCC TGACGGCCAA GGCGGCCACC 
TGGAAGTCGA ACGAGGCGCG CCGTCCGAAA ACGTACAACT GCACGGCCTG CAACAAGTGG 
TTCACCAGCT CGGGCCATCT TAAGCGCCAC TACAATACGA CGCTGCACAA GAATGCGGTT 
AAGTCGAGCG GCCAGCCGGA TCCAGCCACC CTGCCCATCT CGGCGCACCA CCATCCCGCC 
CGCGACCCGG TGACGAAGCC CAGTCGCAGG GGCAATGCCG CAGCCGCTGC GGCCGCCGCT 
GCAGCTGCCG CCTCGGCCAG CGGTCAGGGT CAGCAGCAAC AGCCGCCGAT ACCGCCGCCG 
CCGGCCAATG TGCCACCCCC GGAGCCGCCT AGAAGTCCGC CCGATTATGG CGGAGGAGGC 
GGCTTGGGCG TGGGAGCGAT GGGCGGCGCT GCCATGTCCC AGTACTCGGC CTCACCCTCG 
CCCACACAGC AGCAGCAGCA CCACCTCAAC CACCATCAGC AGCAGGCCAA CGGCTATGCT 
AATGGCACCG CCAACGGCTA TGGCTACATG CAGCAACAAG TGCAATCCAC GACAAACGCT 
TCACCACAGC ACGCTTCCAA CAACAACAGC AACAACCAGC AGCAGCAGCA ACAACAGCAG 
CAGCACCATC AGCAGCAGAT GCCGCAGCAC CACAACTCCG TTTTGAACGG TCACCCAAAC 
GGGCTAGCAG GTCCCTCCGC CCCACACAAC AACAACACCA CCCAAATGCC GTCCTCCCAA 
ATGAGGGGCC TGCTGAACGA AACAACAACC ACGCCGCCAC CAACAACAAC AACCCGAGCA 
CCACAAATCA CAACAACAGC AACAACAACA ACAACGGCGG CCACCACGGT AGCTATAAAG 
AGCGAGCAAA TGGAGGACAG CAACCACACT CACACCCACA CACACACGCA TCCCAATCAC 
AGTCACAGCC AGGACAGGAG CCACAGCAGC AGCAGCAGCA GCTCAATGGC CACGGAGGAG 
GCGGAGGAGC AGGAGCTGCG GGATCAGGAG CAGGCGGACG ATCACCTGCA TCAGCATCAA 
CAGGCGTCGC AGCAGTATCT GCTGTCGGCG CGTCATTACC ACAGCAGCAC GCCCAACACG 
CTCAGCAGCA GCAACACCAA TCCCAGCACG CCCAGCAGCA ACTCGCCCCA TACTATCTAC 
CGGCAGGAGC AGCAGGGAAC GGATTTCTCG CGCACCACCC CGCCGCCGCA GCCGCTGCCG 
CCTATGGGAA TGCTGCCGCC TATGGCAATG GACTACAACA TGCTGGCTTT GGATATGCCC 
ATGCCCATGC CCACGCTCAT GCACAGCAAT ATGCTGCAGT GCAGCAGCAC CAGCACCACG 
CCGCTAGCTA CTACCATCAC CACCAGTATG CCGGACACTA TGCAGCCGCC GCAGCAGCAG 
CTGGTGCACC ACTACCAGGC GGTGCTCCAC CCGCTCCATC AGCAGCTGGG GGAGCAGCAC 
CAGCGCCAGG AGGCGGATCA TCATCAGCAG CAGCGGGAGC TTCACCAACT GGATCAGCAG 
CAGCAGCAGC AGCAGGCGTT AATCCTGGCG GACAGTTTGC CGCACAGCAG CAGTTCGCCC 
ACCAGCAGCT CCCCACCGCC CACCATGCCC ATGCCGCTCA CCACCATCAC AGCGCCGCAG 
CTGCTGCCGC TGCAGCCGCC GCCGCCGCAC ATCACCAGCA CCATGCCCAT GCCGCCCACC 
ATGCACATGC CCATCATGCC GCCGCCGCCG CAATGCTACC AGCAGCTGCA GCCGCTGGAC 
CCCACAATGA GCTATCACAC TATTATTGGT AGTGGCCCGG AGGCGCATAC CGGAACGGCC 
GGCGGTGGCT ATAGCAATCA GATCACCACC AGCGATGGCC AGATCCTGCA GCTGATGCCA 
ACATCCCTGT TCGCGCCATA TGCACCGCTC TCGCCGTACT CGGTGGCCGC CCAGCGATCG 
CCGCAGGAGG GCGATCTGCC GCCGGTTCAC ACACTCACGA CGGCCCTGCA TGCCCATCAG 
CAGGGCGGGC AGCAGGAGGC GCAGACGCCA ACGCTGACTG TGCTCTCCAC GCCCTACTCG 
CCCACAGTGA GCAGCTCGCG GGCGACTCCC GCACTGGAGA TGGATATGGC CACGCTGATG 
CAGCACCATC AGGACTATGA GATGGAGCAG TACCAGATGC AGCACCAGCA GTTGGATCAG 
ATGCAGCAGC ACCAGCAGCA ACTGGACCAC CAGCAGCAGC AACAGATCCT GGCGGATCAG 
ACCCAGACGA TGGCCCAACA GCCGCTGGCC AAGAAGCGGC GTGGCGGCAA CGCAACACCA 
TCGACGACGA AGCGACGGCG GAACAGCAGC GTCGGATCGA CGTCGCCGCA CTCGACCACC 
CTGCCATCGG GACGGATCAA GTGTCTGGAG TGCGACAAGG AGTTCACCAA GAACTGCTAC 
CTCACGCAGC ACAACAAGAG CTTCCACTCC GTGAGTTTGC CATATGTATG TGTGAAACGA 
TTGAAAGACA GCCAGAATTC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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zld cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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