Cdc42 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cdc42
Entrez Gene ID	32981
Full Name	CG12530 gene product from transcript CG12530-RC
Synonyms	CDC42,CG12530,Cdc42Dm,D-CDC42,D-Cdc42,D-cdc42,DCDC42,DCdc42,Dcdc42,Dm Cdc42,DmCDC42,Dmcdc42,Dmel\CG12530,P40793,cdc42
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 18E1-18E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001258834.3	NP_001245763.1	Cdc42, isoform D
NM_001258833.3	NP_001245762.1	Cdc42, isoform C
NM_078690.4	NP_523414.1	Cdc42, isoform A

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CDC42	XP_003951460.1
Canis lupus familiaris (dog)	CDC42	NP_001003254.1
Saccharomyces cerevisiae (baker's yeast)	CDC42	NP_013330.1
Neurospora crassa	NCU06454	XP_957345.2
Xenopus tropicalis (tropical clawed frog)	cdc42	NP_001017070.1
Homo sapiens (human)	CDC42	NP_001034891.1
Danio rerio (zebrafish)	cdc42l	NP_956159.1
Magnaporthe oryzae (rice blast fungus)	MGG_00466	XP_003718598.1
	LOC100424807	XP_002803768.1
Caenorhabditis elegans (roundworm)	cdc-42	NP_495598.1
Oryza sativa (rice)	Os01g0229400	NP_001042486.1
Anopheles gambiae (African malaria mosquito)	CDC42_ANOGA	XP_003436126.1
Kluyveromyces lactis	KLLA0A04213g	XP_451186.1
Eremothecium gossypii	AGOS_AGL093W	NP_986573.1
Bos taurus (cattle)	CDC42	NP_001039797.1
Gallus gallus (chicken)	CDC42	NP_990379.1
Drosophila melanogaster (fruit fly)	Cdc42	NP_001245764.1
Schizosaccharomyces pombe (fission yeast)	cdc42	NP_593536.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Cdc42 controls protrusion formation in a cell non-autonomous manner.
Title: Non-autonomous role of Cdc42 in cell-cell communication during collective migration.

Cdc42 regulates binding of Crumbs protein to the Par-6 CRIB-PDZ module.
Title: Binding of Crumbs to the Par-6 CRIB-PDZ Module Is Regulated by Cdc42.

We propose that the guanine nucleotide exchange factor GEFmeso is involved in a developmental process that requires the synergistic action of CDC42 and Rac1 during Drosophila development
Title: Rac1 is a novel interactor of Drosophila guanine nucleotide exchange factor GEFmeso.

The distinct buttoning mechanism we propose for dorsal vessel closure is elaborated through signaling pathways regulating Cdc42 activity in this cell type.
Title: Cdc42 is required in a genetically distinct subset of cardiac cells during Drosophila dorsal vessel closure.

A proof-of-principle experiment in which the Cdc42 GTPase associates with its alleged partner WASp within neurons during the time and space that coincide with the newly developing CNS.
Title: Imaging dynamic molecular signaling by the Cdc42 GTPase within the developing CNS.

The following Cdc42 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cdc42 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa02169	NM_078690.4 Latest version!	Drosophila melanogaster Cdc42, transcript variant A (Cdc42), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa02169	NM_001258833.3 Latest version!	Drosophila melanogaster Cdc42, transcript variant C (Cdc42), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa02169	NM_001258834.3 Latest version!	Drosophila melanogaster Cdc42, transcript variant D (Cdc42), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cdc42 ( NM_001258834.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245763.1 Drosophila melanogaster Cdc42, transcript variant D (Cdc42), mRNA.

Price & Availability↑

CloneID	OFa02169
Clone ID Related Accession (Same CDS sequence)	NM_001258834.3 , NM_001258833.3 , NM_078690.4
Accession Version	NM_001258834.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Cdc42, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001258834.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAAACCA TCAAGTGCGT GGTCGTCGGC GACGGAGCCG TGGGTAAGAC ATGCCTGCTC 
ATCTCGTATA CAACCAACAA GTTCCCGTCG GAGTACGTGC CCACGGTGTT CGACAACTAT 
GCGGTCACTG TGATGATCGG CGGTGAGCCC TACACACTGG GCCTGTTCGA TACGGCCGGA 
CAGGAGGACT ACGATCGGCT GCGTCCGCTC TCCTATCCGC AGACGGATGT CTTCCTTGTC 
TGCTTTTCGG TGGTCAGTCC CAGTTCCTTC GAGAACGTCA AGGAGAAGTG GGTGCCCGAG 
ATTACACACC ATTGCCAAAA GACGCCGTTC CTGCTGGTGG GCACACAGAT TGATTTGCGC 
GACGAGAACA GCACGCTGGA GAAGCTGGCC AAGAACAAGC AGAAGCCCAT CACCATGGAG 
CAGGGCGAGA AGCTGGCCAA GGAGCTGAAG GCCGTCAAGT ACGTGGAGTG CTCGGCCTTG 
ACACAGAAGG GCCTGAAAAA TGTATTCGAC GAGGCCATCC TGGCCGCCCT CGAGCCACCA 
GAGCCCACAA AGAAAAGGAA GTGCAAATTC TTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245763.1
CDS	86..661
Translation	MQTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVMIGGEPYTLGLFDTAGQEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHHCQKTPFLLVGTQIDLRDENSTLEKLAKNKQKPITMEQGEKLAKELKAVKYVECSALTQKGLKNVFDEAILAALEPPEPTKKRKCKFL

Target ORF information:

RefSeq Version	NM_001258834.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Cdc42, transcript variant D (Cdc42), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258834.3

ORF Insert Sequence:

ATGCAAACCA TCAAGTGCGT GGTCGTCGGC GACGGAGCCG TGGGTAAGAC ATGCCTGCTC 
ATCTCGTATA CAACCAACAA GTTCCCGTCG GAGTACGTGC CCACGGTGTT CGACAACTAT 
GCGGTCACTG TGATGATCGG CGGTGAGCCC TACACACTGG GCCTGTTCGA TACGGCCGGA 
CAGGAGGACT ACGATCGGCT GCGTCCGCTC TCCTATCCGC AGACGGATGT CTTCCTTGTC 
TGCTTTTCGG TGGTCAGTCC CAGTTCCTTC GAGAACGTCA AGGAGAAGTG GGTGCCCGAG 
ATTACACACC ATTGCCAAAA GACGCCGTTC CTGCTGGTGG GCACACAGAT TGATTTGCGC 
GACGAGAACA GCACGCTGGA GAAGCTGGCC AAGAACAAGC AGAAGCCCAT CACCATGGAG 
CAGGGCGAGA AGCTGGCCAA GGAGCTGAAG GCCGTCAAGT ACGTGGAGTG CTCGGCCTTG 
ACACAGAAGG GCCTGAAAAA TGTATTCGAC GAGGCCATCC TGGCCGCCCT CGAGCCACCA 
GAGCCCACAA AGAAAAGGAA GTGCAAATTC TTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc42 ( NM_001258833.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245762.1 Drosophila melanogaster Cdc42, transcript variant C (Cdc42), mRNA.

Price & Availability↑

CloneID	OFa02169
Clone ID Related Accession (Same CDS sequence)	NM_001258834.3 , NM_001258833.3 , NM_078690.4
Accession Version	NM_001258833.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Cdc42, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001258833.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAAACCA TCAAGTGCGT GGTCGTCGGC GACGGAGCCG TGGGTAAGAC ATGCCTGCTC 
ATCTCGTATA CAACCAACAA GTTCCCGTCG GAGTACGTGC CCACGGTGTT CGACAACTAT 
GCGGTCACTG TGATGATCGG CGGTGAGCCC TACACACTGG GCCTGTTCGA TACGGCCGGA 
CAGGAGGACT ACGATCGGCT GCGTCCGCTC TCCTATCCGC AGACGGATGT CTTCCTTGTC 
TGCTTTTCGG TGGTCAGTCC CAGTTCCTTC GAGAACGTCA AGGAGAAGTG GGTGCCCGAG 
ATTACACACC ATTGCCAAAA GACGCCGTTC CTGCTGGTGG GCACACAGAT TGATTTGCGC 
GACGAGAACA GCACGCTGGA GAAGCTGGCC AAGAACAAGC AGAAGCCCAT CACCATGGAG 
CAGGGCGAGA AGCTGGCCAA GGAGCTGAAG GCCGTCAAGT ACGTGGAGTG CTCGGCCTTG 
ACACAGAAGG GCCTGAAAAA TGTATTCGAC GAGGCCATCC TGGCCGCCCT CGAGCCACCA 
GAGCCCACAA AGAAAAGGAA GTGCAAATTC TTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245762.1
CDS	159..734
Translation	MQTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVMIGGEPYTLGLFDTAGQEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHHCQKTPFLLVGTQIDLRDENSTLEKLAKNKQKPITMEQGEKLAKELKAVKYVECSALTQKGLKNVFDEAILAALEPPEPTKKRKCKFL

Target ORF information:

RefSeq Version	NM_001258833.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Cdc42, transcript variant C (Cdc42), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258833.3

ORF Insert Sequence:

ATGCAAACCA TCAAGTGCGT GGTCGTCGGC GACGGAGCCG TGGGTAAGAC ATGCCTGCTC 
ATCTCGTATA CAACCAACAA GTTCCCGTCG GAGTACGTGC CCACGGTGTT CGACAACTAT 
GCGGTCACTG TGATGATCGG CGGTGAGCCC TACACACTGG GCCTGTTCGA TACGGCCGGA 
CAGGAGGACT ACGATCGGCT GCGTCCGCTC TCCTATCCGC AGACGGATGT CTTCCTTGTC 
TGCTTTTCGG TGGTCAGTCC CAGTTCCTTC GAGAACGTCA AGGAGAAGTG GGTGCCCGAG 
ATTACACACC ATTGCCAAAA GACGCCGTTC CTGCTGGTGG GCACACAGAT TGATTTGCGC 
GACGAGAACA GCACGCTGGA GAAGCTGGCC AAGAACAAGC AGAAGCCCAT CACCATGGAG 
CAGGGCGAGA AGCTGGCCAA GGAGCTGAAG GCCGTCAAGT ACGTGGAGTG CTCGGCCTTG 
ACACAGAAGG GCCTGAAAAA TGTATTCGAC GAGGCCATCC TGGCCGCCCT CGAGCCACCA 
GAGCCCACAA AGAAAAGGAA GTGCAAATTC TTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc42 ( NM_078690.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523414.1 Drosophila melanogaster Cdc42, transcript variant A (Cdc42), mRNA.

Price & Availability↑

CloneID	OFa02169
Clone ID Related Accession (Same CDS sequence)	NM_001258834.3 , NM_001258833.3 , NM_078690.4
Accession Version	NM_078690.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Cdc42, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_078690.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAAACCA TCAAGTGCGT GGTCGTCGGC GACGGAGCCG TGGGTAAGAC ATGCCTGCTC 
ATCTCGTATA CAACCAACAA GTTCCCGTCG GAGTACGTGC CCACGGTGTT CGACAACTAT 
GCGGTCACTG TGATGATCGG CGGTGAGCCC TACACACTGG GCCTGTTCGA TACGGCCGGA 
CAGGAGGACT ACGATCGGCT GCGTCCGCTC TCCTATCCGC AGACGGATGT CTTCCTTGTC 
TGCTTTTCGG TGGTCAGTCC CAGTTCCTTC GAGAACGTCA AGGAGAAGTG GGTGCCCGAG 
ATTACACACC ATTGCCAAAA GACGCCGTTC CTGCTGGTGG GCACACAGAT TGATTTGCGC 
GACGAGAACA GCACGCTGGA GAAGCTGGCC AAGAACAAGC AGAAGCCCAT CACCATGGAG 
CAGGGCGAGA AGCTGGCCAA GGAGCTGAAG GCCGTCAAGT ACGTGGAGTG CTCGGCCTTG 
ACACAGAAGG GCCTGAAAAA TGTATTCGAC GAGGCCATCC TGGCCGCCCT CGAGCCACCA 
GAGCCCACAA AGAAAAGGAA GTGCAAATTC TTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523414.1
CDS	159..734
Translation	MQTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVMIGGEPYTLGLFDTAGQEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHHCQKTPFLLVGTQIDLRDENSTLEKLAKNKQKPITMEQGEKLAKELKAVKYVECSALTQKGLKNVFDEAILAALEPPEPTKKRKCKFL

Target ORF information:

RefSeq Version	NM_078690.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Cdc42, transcript variant A (Cdc42), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078690.4

ORF Insert Sequence:

ATGCAAACCA TCAAGTGCGT GGTCGTCGGC GACGGAGCCG TGGGTAAGAC ATGCCTGCTC 
ATCTCGTATA CAACCAACAA GTTCCCGTCG GAGTACGTGC CCACGGTGTT CGACAACTAT 
GCGGTCACTG TGATGATCGG CGGTGAGCCC TACACACTGG GCCTGTTCGA TACGGCCGGA 
CAGGAGGACT ACGATCGGCT GCGTCCGCTC TCCTATCCGC AGACGGATGT CTTCCTTGTC 
TGCTTTTCGG TGGTCAGTCC CAGTTCCTTC GAGAACGTCA AGGAGAAGTG GGTGCCCGAG 
ATTACACACC ATTGCCAAAA GACGCCGTTC CTGCTGGTGG GCACACAGAT TGATTTGCGC 
GACGAGAACA GCACGCTGGA GAAGCTGGCC AAGAACAAGC AGAAGCCCAT CACCATGGAG 
CAGGGCGAGA AGCTGGCCAA GGAGCTGAAG GCCGTCAAGT ACGTGGAGTG CTCGGCCTTG 
ACACAGAAGG GCCTGAAAAA TGTATTCGAC GAGGCCATCC TGGCCGCCCT CGAGCCACCA 
GAGCCCACAA AGAAAAGGAA GTGCAAATTC TTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Cdc42 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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