car cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	car
Entrez Gene ID	32947
Full Name	carnation
Synonyms	CG12230,Car,Dm-Vps33,Dmel\CG12230,VPS33a,Vps33,Vps33/carnation,Vps33A,Vps33a,Vps33p,carn,l(1)G0447
General protein information	Preferred Names carnation Names CG12230 gene product from transcript CG12230-RB CG12230-PA CG12230-PB CG12230-PC car-PA car-PB car-PC vacuolar protein sorting protein 33
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 18D1-18D1\|1-62.5 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_078686.5	NP_523410.1	carnation, isoform A
NM_167664.2	NP_728266.1	carnation, isoform B
NM_001272778.1	NP_001259707.1	carnation, isoform C

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	vps33a	NP_001093443.1
Drosophila melanogaster (fruit fly)	car	NP_001259707.1
Magnaporthe oryzae (rice blast fungus)	MGG_09914	XP_003710012.1
Arabidopsis thaliana (thale cress)	ATVPS33	NP_001154679.1
Oryza sativa (rice)	Os04g0223000	NP_001052270.1
Homo sapiens (human)	VPS33A	NP_075067.2
Pan troglodytes (chimpanzee)	VPS33A	XP_001167604.1
	VPS33A	XP_001098683.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003203	XP_312907.4
Canis lupus familiaris (dog)	VPS33A	XP_534660.2
Gallus gallus (chicken)	VPS33A	XP_415153.1
Bos taurus (cattle)	VPS33A	NP_001039514.1
Rattus norvegicus (Norway rat)	Vps33a	NP_075250.2
Neurospora crassa	NCU09121	XP_958893.1
Mus musculus (house mouse)	Vps33a	NP_084205.3
Schizosaccharomyces pombe (fission yeast)	vps33	NP_596210.1
Xenopus tropicalis (tropical clawed frog)	vps33a	NP_001016935.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

dVps33B predominantly binds the early endosomal Avl and Car to dSyntaxin16
Title: The SM protein Car/Vps33A regulates SNARE-mediated trafficking to lysosomes and lysosome-related organelles.

The following car gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the car cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa02135	NM_078686.5 Latest version!	Drosophila melanogaster carnation (car), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa02135	NM_167664.2 Latest version!	Drosophila melanogaster carnation (car), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa02953	NM_001272778.1 Latest version!	Drosophila melanogaster carnation (car), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

car ( NM_078686.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523410.1 Drosophila melanogaster carnation (car), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa02135
Clone ID Related Accession (Same CDS sequence)	NM_078686.5 , NM_167664.2
Accession Version	NM_078686.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	carnation, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_078686.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCCCCC ACTTGAAGGG CCATGGTCAG CGGGTCAACC TGCAGTTGCT GCAGGAGGCC 
GCCTGCCGCG AGCTGCTGCA GCAGCTGGAC CGCATTGAGG GTTCCAAGGT TATTGTGCTG 
GACGAGACCA TGATCGGACC GCTGGACTTG GTTACCCGGC CAAAGTTATT CGCTGATCGA 
GGCATCCGTC TGCTGGCCCT CAAGCCGGAG CTTCATTTGC CGCGCGAGGT GGCCAATGTG 
GTGTACGTGA TGCGCCCACG CGTGGCGCTG ATGGAGCAGC TGGCCGCCCA CGTGAAGGCA 
GGCGGAAGAG CGGCCGCTGG ACGGCAGTAC CACATCCTGT TCGCCCCGAG GCGGTCATGT 
CTGTGCGTCA GCCAACTGGA GGTCAGCGGC GTGTTGGGCA GCTTCGGAAA CATCGAGGAA 
CTGGCCTGGA ACTATCTGCC GCTGGATGTC GACCTGGTAT CGATGGAGAT GCCCAATGCC 
TTCCGCGATG TGAGTGTGGA CGGTGACACT AGCTCGCTGT ATCAGGCGGC AGTGGGCCTA 
GTGCAGCTGC AGCGTCTATA CGGTCGCATT CCGAAGATCT ACGGAAAAGG AGAGTTTGCG 
CACAGGGTAT GGGAGCACGC CAAGCAGCTG GGCCGGGATG AGCGGACTCT GTACAACGGC 
GACAAGGGTG TCGTCGATCA GTTGATCCTT CTGGACAGAA GCATCGATCT GCTCAGCCCG 
CTGGCCACAC AACTCACCTA CGAGGGGCTT ATCGATGAGT TCTACGGCAT CCGGCAGAAC 
AAACTGACGC TGCCCGCCGA GAACTTTCCC TCTGACGGAG CCCTTCCCGG AGGCGGAGGC 
AGTGGCCCAC GAGTCGAGGA ATCGCAGTCG CTGCTGGGTG ACACCGAGAA AAAGACCATC 
CTACTGCACT CCGGCGAGCA GTTGTACGCG GAGCTGCGGA ACAAGCACTT CAACGAGGTC 
ACCAAGCTGC TCGCTCGCAA GGCACGGGAA ATTCACGTCC AGATGCACGC CACCTCCCAG 
GACAAGAGCG TGCAGGAGAT CAAGAGCTTC GTCGAGAACC TCCTGCCGCA GCTAATGGCC 
CAGAAGAAGG CCACCTCTGA GCATACGGCC ATCGCTGGCC TCCTGCACGA ACAGGTGAAC 
GCGGTGCGCT TCGCTGACGA CCTGGCTGCC GAGCAGGAGT TCATGGTCTG CGCAGATATC 
GACAAGCCAA GCGCCTACAT CGAGGACCTG ATTGCCTGCA GGGTGGAGTT GAATCGCGTC 
CTGCGGCTCA TCTGCATGCA GTGCCACGCC GCCTCCGGAT TCAAGGAGAA GCTGCTTAAC 
CACTACAAGC GCGAACTGGT CCATGTGTAC GGTCTGGAAG TGCTGCTCAC CATCAGCAAC 
CTGGAGAAAT CGGGCCTGCT CCATCTGCAA ACAGAGTCGC GGGCTTACAG CGTTCTGCGA 
AAGACGCTGC ACCTCACGGT GGACGACAAC GTTGAAATTG AGCCAAAGGA CATCAGCTAC 
GTGCACAGCT TCTACGCTCC GCTGACCGCG CGCATTGTGG AGCATTCGCT CAAACCGCTG 
GGTTGGCAGA CGCTTAAGAG CCAAATCAAT AACCTGCCCG GACCGACGTT CGAGGACTTT 
CAGGCGCAGT TGGTGGGCAT CGGTGGACGG CATACGGTCA CCACAGTCAG CGAGGGATCC 
CTACTGAATG TGCCGCGAGT GGTGCTCGTC TGCTTTGTGG GCGGATGCAC GTTCGCTGAA 
ATAGCTGCGC TGCGTTTCCT CGCCGCCCAG GAGGACAACA ACGTGGAGTT CCTGATTGCC 
ACCACGAAGG TGGTCAACAA ACACTCCTTC CTGGACAGCC TGATGAGCTC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523410.1
CDS	160..2013
Translation	MFPHLKGHGQRVNLQLLQEAACRELLQQLDRIEGSKVIVLDETMIGPLDLVTRPKLFADRGIRLLALKPELHLPREVANVVYVMRPRVALMEQLAAHVKAGGRAAAGRQYHILFAPRRSCLCVSQLEVSGVLGSFGNIEELAWNYLPLDVDLVSMEMPNAFRDVSVDGDTSSLYQAAVGLVQLQRLYGRIPKIYGKGEFAHRVWEHAKQLGRDERTLYNGDKGVVDQLILLDRSIDLLSPLATQLTYEGLIDEFYGIRQNKLTLPAENFPSDGALPGGGGSGPRVEESQSLLGDTEKKTILLHSGEQLYAELRNKHFNEVTKLLARKAREIHVQMHATSQDKSVQEIKSFVENLLPQLMAQKKATSEHTAIAGLLHEQVNAVRFADDLAAEQEFMVCADIDKPSAYIEDLIACRVELNRVLRLICMQCHAASGFKEKLLNHYKRELVHVYGLEVLLTISNLEKSGLLHLQTESRAYSVLRKTLHLTVDDNVEIEPKDISYVHSFYAPLTARIVEHSLKPLGWQTLKSQINNLPGPTFEDFQAQLVGIGGRHTVTTVSEGSLLNVPRVVLVCFVGGCTFAEIAALRFLAAQEDNNVEFLIATTKVVNKHSFLDSLMSS

Target ORF information:

RefSeq Version	NM_078686.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster carnation (car), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078686.5

ORF Insert Sequence:

ATGTTCCCCC ACTTGAAGGG CCATGGTCAG CGGGTCAACC TGCAGTTGCT GCAGGAGGCC 
GCCTGCCGCG AGCTGCTGCA GCAGCTGGAC CGCATTGAGG GTTCCAAGGT TATTGTGCTG 
GACGAGACCA TGATCGGACC GCTGGACTTG GTTACCCGGC CAAAGTTATT CGCTGATCGA 
GGCATCCGTC TGCTGGCCCT CAAGCCGGAG CTTCATTTGC CGCGCGAGGT GGCCAATGTG 
GTGTACGTGA TGCGCCCACG CGTGGCGCTG ATGGAGCAGC TGGCCGCCCA CGTGAAGGCA 
GGCGGAAGAG CGGCCGCTGG ACGGCAGTAC CACATCCTGT TCGCCCCGAG GCGGTCATGT 
CTGTGCGTCA GCCAACTGGA GGTCAGCGGC GTGTTGGGCA GCTTCGGAAA CATCGAGGAA 
CTGGCCTGGA ACTATCTGCC GCTGGATGTC GACCTGGTAT CGATGGAGAT GCCCAATGCC 
TTCCGCGATG TGAGTGTGGA CGGTGACACT AGCTCGCTGT ATCAGGCGGC AGTGGGCCTA 
GTGCAGCTGC AGCGTCTATA CGGTCGCATT CCGAAGATCT ACGGAAAAGG AGAGTTTGCG 
CACAGGGTAT GGGAGCACGC CAAGCAGCTG GGCCGGGATG AGCGGACTCT GTACAACGGC 
GACAAGGGTG TCGTCGATCA GTTGATCCTT CTGGACAGAA GCATCGATCT GCTCAGCCCG 
CTGGCCACAC AACTCACCTA CGAGGGGCTT ATCGATGAGT TCTACGGCAT CCGGCAGAAC 
AAACTGACGC TGCCCGCCGA GAACTTTCCC TCTGACGGAG CCCTTCCCGG AGGCGGAGGC 
AGTGGCCCAC GAGTCGAGGA ATCGCAGTCG CTGCTGGGTG ACACCGAGAA AAAGACCATC 
CTACTGCACT CCGGCGAGCA GTTGTACGCG GAGCTGCGGA ACAAGCACTT CAACGAGGTC 
ACCAAGCTGC TCGCTCGCAA GGCACGGGAA ATTCACGTCC AGATGCACGC CACCTCCCAG 
GACAAGAGCG TGCAGGAGAT CAAGAGCTTC GTCGAGAACC TCCTGCCGCA GCTAATGGCC 
CAGAAGAAGG CCACCTCTGA GCATACGGCC ATCGCTGGCC TCCTGCACGA ACAGGTGAAC 
GCGGTGCGCT TCGCTGACGA CCTGGCTGCC GAGCAGGAGT TCATGGTCTG CGCAGATATC 
GACAAGCCAA GCGCCTACAT CGAGGACCTG ATTGCCTGCA GGGTGGAGTT GAATCGCGTC 
CTGCGGCTCA TCTGCATGCA GTGCCACGCC GCCTCCGGAT TCAAGGAGAA GCTGCTTAAC 
CACTACAAGC GCGAACTGGT CCATGTGTAC GGTCTGGAAG TGCTGCTCAC CATCAGCAAC 
CTGGAGAAAT CGGGCCTGCT CCATCTGCAA ACAGAGTCGC GGGCTTACAG CGTTCTGCGA 
AAGACGCTGC ACCTCACGGT GGACGACAAC GTTGAAATTG AGCCAAAGGA CATCAGCTAC 
GTGCACAGCT TCTACGCTCC GCTGACCGCG CGCATTGTGG AGCATTCGCT CAAACCGCTG 
GGTTGGCAGA CGCTTAAGAG CCAAATCAAT AACCTGCCCG GACCGACGTT CGAGGACTTT 
CAGGCGCAGT TGGTGGGCAT CGGTGGACGG CATACGGTCA CCACAGTCAG CGAGGGATCC 
CTACTGAATG TGCCGCGAGT GGTGCTCGTC TGCTTTGTGG GCGGATGCAC GTTCGCTGAA 
ATAGCTGCGC TGCGTTTCCT CGCCGCCCAG GAGGACAACA ACGTGGAGTT CCTGATTGCC 
ACCACGAAGG TGGTCAACAA ACACTCCTTC CTGGACAGCC TGATGAGCTC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

car ( NM_167664.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_728266.1 Drosophila melanogaster carnation (car), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa02135
Clone ID Related Accession (Same CDS sequence)	NM_078686.5 , NM_167664.2
Accession Version	NM_167664.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	carnation, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Dec 17, 2009 this sequence version replaced NM_167664.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCCCCC ACTTGAAGGG CCATGGTCAG CGGGTCAACC TGCAGTTGCT GCAGGAGGCC 
GCCTGCCGCG AGCTGCTGCA GCAGCTGGAC CGCATTGAGG GTTCCAAGGT TATTGTGCTG 
GACGAGACCA TGATCGGACC GCTGGACTTG GTTACCCGGC CAAAGTTATT CGCTGATCGA 
GGCATCCGTC TGCTGGCCCT CAAGCCGGAG CTTCATTTGC CGCGCGAGGT GGCCAATGTG 
GTGTACGTGA TGCGCCCACG CGTGGCGCTG ATGGAGCAGC TGGCCGCCCA CGTGAAGGCA 
GGCGGAAGAG CGGCCGCTGG ACGGCAGTAC CACATCCTGT TCGCCCCGAG GCGGTCATGT 
CTGTGCGTCA GCCAACTGGA GGTCAGCGGC GTGTTGGGCA GCTTCGGAAA CATCGAGGAA 
CTGGCCTGGA ACTATCTGCC GCTGGATGTC GACCTGGTAT CGATGGAGAT GCCCAATGCC 
TTCCGCGATG TGAGTGTGGA CGGTGACACT AGCTCGCTGT ATCAGGCGGC AGTGGGCCTA 
GTGCAGCTGC AGCGTCTATA CGGTCGCATT CCGAAGATCT ACGGAAAAGG AGAGTTTGCG 
CACAGGGTAT GGGAGCACGC CAAGCAGCTG GGCCGGGATG AGCGGACTCT GTACAACGGC 
GACAAGGGTG TCGTCGATCA GTTGATCCTT CTGGACAGAA GCATCGATCT GCTCAGCCCG 
CTGGCCACAC AACTCACCTA CGAGGGGCTT ATCGATGAGT TCTACGGCAT CCGGCAGAAC 
AAACTGACGC TGCCCGCCGA GAACTTTCCC TCTGACGGAG CCCTTCCCGG AGGCGGAGGC 
AGTGGCCCAC GAGTCGAGGA ATCGCAGTCG CTGCTGGGTG ACACCGAGAA AAAGACCATC 
CTACTGCACT CCGGCGAGCA GTTGTACGCG GAGCTGCGGA ACAAGCACTT CAACGAGGTC 
ACCAAGCTGC TCGCTCGCAA GGCACGGGAA ATTCACGTCC AGATGCACGC CACCTCCCAG 
GACAAGAGCG TGCAGGAGAT CAAGAGCTTC GTCGAGAACC TCCTGCCGCA GCTAATGGCC 
CAGAAGAAGG CCACCTCTGA GCATACGGCC ATCGCTGGCC TCCTGCACGA ACAGGTGAAC 
GCGGTGCGCT TCGCTGACGA CCTGGCTGCC GAGCAGGAGT TCATGGTCTG CGCAGATATC 
GACAAGCCAA GCGCCTACAT CGAGGACCTG ATTGCCTGCA GGGTGGAGTT GAATCGCGTC 
CTGCGGCTCA TCTGCATGCA GTGCCACGCC GCCTCCGGAT TCAAGGAGAA GCTGCTTAAC 
CACTACAAGC GCGAACTGGT CCATGTGTAC GGTCTGGAAG TGCTGCTCAC CATCAGCAAC 
CTGGAGAAAT CGGGCCTGCT CCATCTGCAA ACAGAGTCGC GGGCTTACAG CGTTCTGCGA 
AAGACGCTGC ACCTCACGGT GGACGACAAC GTTGAAATTG AGCCAAAGGA CATCAGCTAC 
GTGCACAGCT TCTACGCTCC GCTGACCGCG CGCATTGTGG AGCATTCGCT CAAACCGCTG 
GGTTGGCAGA CGCTTAAGAG CCAAATCAAT AACCTGCCCG GACCGACGTT CGAGGACTTT 
CAGGCGCAGT TGGTGGGCAT CGGTGGACGG CATACGGTCA CCACAGTCAG CGAGGGATCC 
CTACTGAATG TGCCGCGAGT GGTGCTCGTC TGCTTTGTGG GCGGATGCAC GTTCGCTGAA 
ATAGCTGCGC TGCGTTTCCT CGCCGCCCAG GAGGACAACA ACGTGGAGTT CCTGATTGCC 
ACCACGAAGG TGGTCAACAA ACACTCCTTC CTGGACAGCC TGATGAGCTC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_728266.1
CDS	236..2089
Translation	MFPHLKGHGQRVNLQLLQEAACRELLQQLDRIEGSKVIVLDETMIGPLDLVTRPKLFADRGIRLLALKPELHLPREVANVVYVMRPRVALMEQLAAHVKAGGRAAAGRQYHILFAPRRSCLCVSQLEVSGVLGSFGNIEELAWNYLPLDVDLVSMEMPNAFRDVSVDGDTSSLYQAAVGLVQLQRLYGRIPKIYGKGEFAHRVWEHAKQLGRDERTLYNGDKGVVDQLILLDRSIDLLSPLATQLTYEGLIDEFYGIRQNKLTLPAENFPSDGALPGGGGSGPRVEESQSLLGDTEKKTILLHSGEQLYAELRNKHFNEVTKLLARKAREIHVQMHATSQDKSVQEIKSFVENLLPQLMAQKKATSEHTAIAGLLHEQVNAVRFADDLAAEQEFMVCADIDKPSAYIEDLIACRVELNRVLRLICMQCHAASGFKEKLLNHYKRELVHVYGLEVLLTISNLEKSGLLHLQTESRAYSVLRKTLHLTVDDNVEIEPKDISYVHSFYAPLTARIVEHSLKPLGWQTLKSQINNLPGPTFEDFQAQLVGIGGRHTVTTVSEGSLLNVPRVVLVCFVGGCTFAEIAALRFLAAQEDNNVEFLIATTKVVNKHSFLDSLMSS

Target ORF information:

RefSeq Version	NM_167664.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster carnation (car), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167664.2

ORF Insert Sequence:

ATGTTCCCCC ACTTGAAGGG CCATGGTCAG CGGGTCAACC TGCAGTTGCT GCAGGAGGCC 
GCCTGCCGCG AGCTGCTGCA GCAGCTGGAC CGCATTGAGG GTTCCAAGGT TATTGTGCTG 
GACGAGACCA TGATCGGACC GCTGGACTTG GTTACCCGGC CAAAGTTATT CGCTGATCGA 
GGCATCCGTC TGCTGGCCCT CAAGCCGGAG CTTCATTTGC CGCGCGAGGT GGCCAATGTG 
GTGTACGTGA TGCGCCCACG CGTGGCGCTG ATGGAGCAGC TGGCCGCCCA CGTGAAGGCA 
GGCGGAAGAG CGGCCGCTGG ACGGCAGTAC CACATCCTGT TCGCCCCGAG GCGGTCATGT 
CTGTGCGTCA GCCAACTGGA GGTCAGCGGC GTGTTGGGCA GCTTCGGAAA CATCGAGGAA 
CTGGCCTGGA ACTATCTGCC GCTGGATGTC GACCTGGTAT CGATGGAGAT GCCCAATGCC 
TTCCGCGATG TGAGTGTGGA CGGTGACACT AGCTCGCTGT ATCAGGCGGC AGTGGGCCTA 
GTGCAGCTGC AGCGTCTATA CGGTCGCATT CCGAAGATCT ACGGAAAAGG AGAGTTTGCG 
CACAGGGTAT GGGAGCACGC CAAGCAGCTG GGCCGGGATG AGCGGACTCT GTACAACGGC 
GACAAGGGTG TCGTCGATCA GTTGATCCTT CTGGACAGAA GCATCGATCT GCTCAGCCCG 
CTGGCCACAC AACTCACCTA CGAGGGGCTT ATCGATGAGT TCTACGGCAT CCGGCAGAAC 
AAACTGACGC TGCCCGCCGA GAACTTTCCC TCTGACGGAG CCCTTCCCGG AGGCGGAGGC 
AGTGGCCCAC GAGTCGAGGA ATCGCAGTCG CTGCTGGGTG ACACCGAGAA AAAGACCATC 
CTACTGCACT CCGGCGAGCA GTTGTACGCG GAGCTGCGGA ACAAGCACTT CAACGAGGTC 
ACCAAGCTGC TCGCTCGCAA GGCACGGGAA ATTCACGTCC AGATGCACGC CACCTCCCAG 
GACAAGAGCG TGCAGGAGAT CAAGAGCTTC GTCGAGAACC TCCTGCCGCA GCTAATGGCC 
CAGAAGAAGG CCACCTCTGA GCATACGGCC ATCGCTGGCC TCCTGCACGA ACAGGTGAAC 
GCGGTGCGCT TCGCTGACGA CCTGGCTGCC GAGCAGGAGT TCATGGTCTG CGCAGATATC 
GACAAGCCAA GCGCCTACAT CGAGGACCTG ATTGCCTGCA GGGTGGAGTT GAATCGCGTC 
CTGCGGCTCA TCTGCATGCA GTGCCACGCC GCCTCCGGAT TCAAGGAGAA GCTGCTTAAC 
CACTACAAGC GCGAACTGGT CCATGTGTAC GGTCTGGAAG TGCTGCTCAC CATCAGCAAC 
CTGGAGAAAT CGGGCCTGCT CCATCTGCAA ACAGAGTCGC GGGCTTACAG CGTTCTGCGA 
AAGACGCTGC ACCTCACGGT GGACGACAAC GTTGAAATTG AGCCAAAGGA CATCAGCTAC 
GTGCACAGCT TCTACGCTCC GCTGACCGCG CGCATTGTGG AGCATTCGCT CAAACCGCTG 
GGTTGGCAGA CGCTTAAGAG CCAAATCAAT AACCTGCCCG GACCGACGTT CGAGGACTTT 
CAGGCGCAGT TGGTGGGCAT CGGTGGACGG CATACGGTCA CCACAGTCAG CGAGGGATCC 
CTACTGAATG TGCCGCGAGT GGTGCTCGTC TGCTTTGTGG GCGGATGCAC GTTCGCTGAA 
ATAGCTGCGC TGCGTTTCCT CGCCGCCCAG GAGGACAACA ACGTGGAGTT CCTGATTGCC 
ACCACGAAGG TGGTCAACAA ACACTCCTTC CTGGACAGCC TGATGAGCTC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

car ( NM_001272778.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259707.1 Drosophila melanogaster carnation (car), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa02953
Clone ID Related Accession (Same CDS sequence)	NM_001272778.1
Accession Version	NM_001272778.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1920bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	carnation, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCCCCC ACTTGAAGGG CCATGGTCAG CGGGTCAACC TGCAGTTGCT GCAGGAGGCC 
GCCTGCCGCG AGCTGCTGCA GCAGCTGGAC CGCATTGAGG GTTCCAAGGT TATTGTGCTG 
GACGAGACCA TGATCGGACC GCTGGACTTG GTTACCCGGC CAAAGTTATT CGCTGATCGA 
GGCATCCGTC TGCTGGCCCT CAAGCCGGAG CTTCATTTGC CGCGCGAGGT GGCCAATGTG 
GTGTACGTGA TGCGCCCACG CGTGGCGCTG ATGGAGCAGC TGGCCGCCCA CGTGAAGGCA 
GGCGGAAGAG CGGCCGCTGG ACGGCAGTAC CACATCCTGT TCGCCCCGAG GCGGTCATGT 
CTGTGCGTCA GCCAACTGGA GGTCAGCGGC GTGTTGGGCA GCTTCGGAAA CATCGAGGAA 
CTGGCCTGGA ACTATCTGCC GCTGGATGTC GACCTGGTAT CGATGGAGAT GCCCAATGCC 
TTCCGCGATG TGAGTGTGGA CGGTGACACT AGCTCGCTGT ATCAGGCGGC AGTGGGCCTA 
GTGCAGCTGC AGCGTCTATA CGGTCGCATT CCGAAGATCT ACGGAAAAGG AGAGTTTGCG 
CACAGGGTAT GGGAGCACGC CAAGCAGCTG GGCCGGGATG AGCGGACTCT GTACAACGGC 
GACAAGGGTG TCGTCGATCA GTTGATCCTT CTGGACAGAA GCATCGATCT GCTCAGCCCG 
CTGGCCACAC AACTCACCTA CGAGGGGCTT ATCGATGAGT TCTACGGCAT CCGGCAGAAC 
AAACTGACGC TGCCCGCCGA GAACTTTCCC TCTGACGGAG CCCTTCCCGG AGGCGGAGGC 
AGTGGCCCAC GAGTCGAGGA ATCGCAGTCG CTGCTGGGTG ACACCGAGAA AAAGACCATC 
CTACTGCACT CCGGCGAGCA GTTGTACGCG GAGCTGCGGA ACAAGCACTT CAACGAGGTC 
ACCAAGCTGC TCGCTCGCAA GGCACGGGAA ATTCACGTCC AGATGCACGC CACCTCCCAG 
GACAAGAGCG TGCAGGAGAT CAAGAGCTTC GTCGAGAACC TCCTGCCGCA GCTAATGGCC 
CAGAAGAAGG CCACCTCTGA GCATACGGCC ATCGCTGGCC TCCTGCACGA ACAGGTGAAC 
GCGGTGCGCT TCGCTGACGA CCTGGCTGCC GAGCAGGAGT TCATGGTCTG CGCAGATATC 
GACAAGCCAA GCGCCTACAT CGAGGACCTG ATTGCCTGCA GGGTGGAGTT GAATCGCGTC 
CTGCGGCTCA TCTGCATGCA GTGCCACGCC GCCTCCGGAT TCAAGGAGAA GCTGCTTAAC 
CACTACAAGC GCGAACTGGT CCATGTGTAC GGTCTGGAAG TGCTGCTCAC CATCAGCAAC 
CTGGAGAAAT CGGGCCTGCT CCATCTGCAA ACAGAGTCGC GGGCTTACAG CGTTCTGCGA 
AAGACGCTGC ACCTCACGGT GGACGACAAC GTTGAAATTG AGCCAAAGGA CATCAGCTAC 
GTGCACAGCT TCTACGCTCC GCTGACCGCG CGCATTGTGG AGCATTCGCT CAAACCGCTG 
GGTTGGCAGA CGCTTAAGAG CCAAATCAAT AACCTGCCCG GACCGACGTT CGAGGACTTT 
CAGGCGCAGT TGGTGGGCAT CGGTGGACGG CATACGGTCA CCACAGTCAG CGAGGGATCC 
CTACTGAATG TGCCGCGAGT GGTGCTCGTC TGCTTTGTGG GCGGATGCAC GTTCGCTGAA 
ATAGCTGCGC TGCGTTTCCT CGCCGCCCAG GAGGACAACA ACGTGGAGTT CCTGATTGCC 
ACCACGAAGG TGGTCAACAA ACACTCCTTC CTGGACAGCC TGATGAGCTC GTGAGGCCGG 
CGGCTAAGCC GACTGGAAGC CTATCGCCTG TCCAACGCCC ACGGCCTGGC ACGTGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259707.1
CDS	236..2155
Translation	MFPHLKGHGQRVNLQLLQEAACRELLQQLDRIEGSKVIVLDETMIGPLDLVTRPKLFADRGIRLLALKPELHLPREVANVVYVMRPRVALMEQLAAHVKAGGRAAAGRQYHILFAPRRSCLCVSQLEVSGVLGSFGNIEELAWNYLPLDVDLVSMEMPNAFRDVSVDGDTSSLYQAAVGLVQLQRLYGRIPKIYGKGEFAHRVWEHAKQLGRDERTLYNGDKGVVDQLILLDRSIDLLSPLATQLTYEGLIDEFYGIRQNKLTLPAENFPSDGALPGGGGSGPRVEESQSLLGDTEKKTILLHSGEQLYAELRNKHFNEVTKLLARKAREIHVQMHATSQDKSVQEIKSFVENLLPQLMAQKKATSEHTAIAGLLHEQVNAVRFADDLAAEQEFMVCADIDKPSAYIEDLIACRVELNRVLRLICMQCHAASGFKEKLLNHYKRELVHVYGLEVLLTISNLEKSGLLHLQTESRAYSVLRKTLHLTVDDNVEIEPKDISYVHSFYAPLTARIVEHSLKPLGWQTLKSQINNLPGPTFEDFQAQLVGIGGRHTVTTVSEGSLLNVPRVVLVCFVGGCTFAEIAALRFLAAQEDNNVEFLIATTKVVNKHSFLDSLMSS*GRRLSRLEAYRLSNAHGLARG

Target ORF information:

RefSeq Version	NM_001272778.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster carnation (car), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272778.1

ORF Insert Sequence:

ATGTTCCCCC ACTTGAAGGG CCATGGTCAG CGGGTCAACC TGCAGTTGCT GCAGGAGGCC 
GCCTGCCGCG AGCTGCTGCA GCAGCTGGAC CGCATTGAGG GTTCCAAGGT TATTGTGCTG 
GACGAGACCA TGATCGGACC GCTGGACTTG GTTACCCGGC CAAAGTTATT CGCTGATCGA 
GGCATCCGTC TGCTGGCCCT CAAGCCGGAG CTTCATTTGC CGCGCGAGGT GGCCAATGTG 
GTGTACGTGA TGCGCCCACG CGTGGCGCTG ATGGAGCAGC TGGCCGCCCA CGTGAAGGCA 
GGCGGAAGAG CGGCCGCTGG ACGGCAGTAC CACATCCTGT TCGCCCCGAG GCGGTCATGT 
CTGTGCGTCA GCCAACTGGA GGTCAGCGGC GTGTTGGGCA GCTTCGGAAA CATCGAGGAA 
CTGGCCTGGA ACTATCTGCC GCTGGATGTC GACCTGGTAT CGATGGAGAT GCCCAATGCC 
TTCCGCGATG TGAGTGTGGA CGGTGACACT AGCTCGCTGT ATCAGGCGGC AGTGGGCCTA 
GTGCAGCTGC AGCGTCTATA CGGTCGCATT CCGAAGATCT ACGGAAAAGG AGAGTTTGCG 
CACAGGGTAT GGGAGCACGC CAAGCAGCTG GGCCGGGATG AGCGGACTCT GTACAACGGC 
GACAAGGGTG TCGTCGATCA GTTGATCCTT CTGGACAGAA GCATCGATCT GCTCAGCCCG 
CTGGCCACAC AACTCACCTA CGAGGGGCTT ATCGATGAGT TCTACGGCAT CCGGCAGAAC 
AAACTGACGC TGCCCGCCGA GAACTTTCCC TCTGACGGAG CCCTTCCCGG AGGCGGAGGC 
AGTGGCCCAC GAGTCGAGGA ATCGCAGTCG CTGCTGGGTG ACACCGAGAA AAAGACCATC 
CTACTGCACT CCGGCGAGCA GTTGTACGCG GAGCTGCGGA ACAAGCACTT CAACGAGGTC 
ACCAAGCTGC TCGCTCGCAA GGCACGGGAA ATTCACGTCC AGATGCACGC CACCTCCCAG 
GACAAGAGCG TGCAGGAGAT CAAGAGCTTC GTCGAGAACC TCCTGCCGCA GCTAATGGCC 
CAGAAGAAGG CCACCTCTGA GCATACGGCC ATCGCTGGCC TCCTGCACGA ACAGGTGAAC 
GCGGTGCGCT TCGCTGACGA CCTGGCTGCC GAGCAGGAGT TCATGGTCTG CGCAGATATC 
GACAAGCCAA GCGCCTACAT CGAGGACCTG ATTGCCTGCA GGGTGGAGTT GAATCGCGTC 
CTGCGGCTCA TCTGCATGCA GTGCCACGCC GCCTCCGGAT TCAAGGAGAA GCTGCTTAAC 
CACTACAAGC GCGAACTGGT CCATGTGTAC GGTCTGGAAG TGCTGCTCAC CATCAGCAAC 
CTGGAGAAAT CGGGCCTGCT CCATCTGCAA ACAGAGTCGC GGGCTTACAG CGTTCTGCGA 
AAGACGCTGC ACCTCACGGT GGACGACAAC GTTGAAATTG AGCCAAAGGA CATCAGCTAC 
GTGCACAGCT TCTACGCTCC GCTGACCGCG CGCATTGTGG AGCATTCGCT CAAACCGCTG 
GGTTGGCAGA CGCTTAAGAG CCAAATCAAT AACCTGCCCG GACCGACGTT CGAGGACTTT 
CAGGCGCAGT TGGTGGGCAT CGGTGGACGG CATACGGTCA CCACAGTCAG CGAGGGATCC 
CTACTGAATG TGCCGCGAGT GGTGCTCGTC TGCTTTGTGG GCGGATGCAC GTTCGCTGAA 
ATAGCTGCGC TGCGTTTCCT CGCCGCCCAG GAGGACAACA ACGTGGAGTT CCTGATTGCC 
ACCACGAAGG TGGTCAACAA ACACTCCTTC CTGGACAGCC TGATGAGCTC GTGAGGCCGG 
CGGCTAAGCC GACTGGAAGC CTATCGCCTG TCCAACGCCC ACGGCCTGGC ACGTGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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car cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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