phm cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	phm
Entrez Gene ID	32857
Full Name	phantom
Synonyms	306a1,CG6578,CYP306A1,Cyp306a1,Cyp306a1-Dm,Dmel\CG6578,PHM,exo,ptm
General protein information	Preferred Names phantom Names CG6578 gene product from transcript CG6578-RB CG6578-PA CG6578-PB cytochrome P450 exocephalon phm-PA phm-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 17D1-17D1\|1-64 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_133091.3	NP_573319.1	phantom, isoform A
NM_001298485.1	NP_001285414.1	phantom, isoform B

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Cyp2j9	NP_083255.1
Rattus norvegicus (Norway rat)	Cyp2j3	NP_786942.1
Gallus gallus (chicken)	CYP2J2	XP_422511.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004665	XP_318345.4
Gallus gallus (chicken)	LOC424676	XP_422509.2
Gallus gallus (chicken)	LOC424677	XP_004936904.1
Drosophila melanogaster (fruit fly)	phm	NP_573319.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Phantom, a cytochrome P450 enzyme essential for ecdysone biosynthesis, plays a critical role in the control of border cell migration in Drosophila.
Title: Phantom, a cytochrome P450 enzyme essential for ecdysone biosynthesis, plays a critical role in the control of border cell migration in Drosophila.

The Drosophila gene phantom (phm)encodes the microsomal 25-hydroxylase (Cyp306a1); strong expression of phm is restricted to the prothoracic gland cells of the Drosophila larval ring gland, and in the adult ovary, but also in the embryonic epidermis.
Title: Phantom encodes the 25-hydroxylase of Drosophila melanogaster and Bombyx mori: a P450 enzyme critical in ecdysone biosynthesis.

CYP306A1 functions as a carbon 25 hydroxylase and has an essential role in ecdysteroid biosynthesis during insect development
Title: CYP306A1, a cytochrome P450 enzyme, is essential for ecdysteroid biosynthesis in the prothoracic glands of Bombyx and Drosophila.

The following phm gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the phm cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa02048	NM_133091.3 Latest version!	Drosophila melanogaster phantom (phm), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa02048	NM_001298485.1 Latest version!	Drosophila melanogaster phantom (phm), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

phm ( NM_133091.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_573319.1 Drosophila melanogaster phantom (phm), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa02048
Clone ID Related Accession (Same CDS sequence)	NM_133091.3 , NM_001298485.1
Accession Version	NM_133091.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phantom, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_133091.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGCGG ACATCGTCGA TATTGGCCAC ACCGGTTGGA TGCCCTCGGT GCAGAGCCTG 
AGTATTCTGC TGGTTCCGGG TGCGCTCGTC CTGGTGATTC TCTACCTGTG CGAGCGCCAG 
TGCAATGACC TCATGGGTGC CCCACCGCCG GGTCCCTGGG GCCTGCCCTT TCTGGGTTAC 
CTGCCCTTCC TGGACGCCCG TGCGCCGCAC AAGTCACTCC AGAAGCTGGC CAAGCGGTAT 
GGTGGAATTT TCGAGCTGAA AATGGGCAGG GTGCCGACCG TAGTCCTCTC GGATGCCGCC 
TTGGTGCGGG ATTTCTTTCG GCGCGATGTG ATGACTGGCC GTGCGCCGCT CTACCTCACC 
CACGGCATCA TGGGTGGATT TGGCATCATC TGCGCCCAGG AGGACATTTG GCGACATGCA 
CGGCGCGAGA CTATCGATTG GCTAAAGGCC TTGGGCATGA CCCGTCGGCC GGGGGAACTG 
CGCGCGCGGC TGGAGCGGCG CATAGCCCGC GGAGTCGACG AGTGCGTACG GCTTTTCGAT 
ACTGAGGCAA AGAAGAGCTG TGCGTCGGAA GTGAATCCGC TGCCGGCGCT CCATCACTCG 
CTGGGCAACA TAATCAACGA CCTGGTCTTC GGGATCACCT ACAAGCGCGA CGACCCCGAC 
TGGCTGTACC TGCAGCGGCT GCAGGAGGAG GGCGTCAAGC TGATTGGCGT CTCCGGGGTG 
GTCAACTTTT TGCCCTGGCT GCGTCACCTG CCCGCCAACG TGCGCAACAT CCGCTTCCTG 
CTGGAGGGCA AGGCCAAGAC GCACGCCATC TACGACCGCA TTGTGGAGGC CTGTGGCCAG 
CGGCTGAAGG AGAAGCAGAA GGTGTTCAAG GAGCTCCAGG AGCAGAAGCG GCTGCAAAGG 
CAGCTAGAGA AGGAGCAGCT CAGGCAGTCA AAGGAAGCGG ATCCAAGCCA GGAGCAGAGT 
GAGGCAGACG AGGATGACGA GGAGAGCGAT GAGGAGGACA CGTACGAGCC GGAGTGCATC 
CTGGAGCACT TCCTAGCCGT TCGAGACACG GATTCGCAGC TCTACTGCGA CGACCAGCTG 
CGCCATCTGC TGGCCGATCT CTTTGGAGCC GGGGTGGACA CCTCGCTGGC CACCCTGCGC 
TGGTTCCTGC TCTACTTGGC CCGCGAACAA CGCTGCCAGC GGCGCCTGCA TGAGCTCCTC 
CTGCCGCTGG GTCCGTCTCC CACTTTGGAG GAACTGGAGC CGCTGGCCTA CCTAAGGGCT 
TGCATTTCCG AGACGATGCG CATACGCAGC GTTGTCCCAC TGGGCATTCC GCACGGATGC 
AAAGAGAACT TCGTCGTGGG CGATTATTTT ATCAAGGGTG GTTCGATGAT CGTTTGCTCG 
GAGTGGGCTA TCCACATGGA CCCAGTGGCC TTCCCGGAAC CGGAGGAGTT CCGTCCGGAG 
CGCTTCTTGA CCGCCGATGG AGCCTACCAG GCGCCGCCAC AGTTCATCCC ATTCTCGTCC 
GGCTATCGAA TGTGTCCCGG CGAAGAGATG GCTCGCATGA TACTCACGCT CTTTACGGGT 
CGCATCCTCA GGCGCTTCCA CTTGGAACTG CCCTCGGGCA CTGAGGTGGA CATGGCGGGT 
GAGAGCGGCA TCACCCTGAC CCCCACTCCG CACATGCTGC GATTCACCAA GCTGCCGGCG 
GTGGAGATGC GCCATGCACC CGACGGAGCT GTGGTGCAGG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_573319.1
CDS	197..1921
Translation	MSADIVDIGHTGWMPSVQSLSILLVPGALVLVILYLCERQCNDLMGAPPPGPWGLPFLGYLPFLDARAPHKSLQKLAKRYGGIFELKMGRVPTVVLSDAALVRDFFRRDVMTGRAPLYLTHGIMGGFGIICAQEDIWRHARRETIDWLKALGMTRRPGELRARLERRIARGVDECVRLFDTEAKKSCASEVNPLPALHHSLGNIINDLVFGITYKRDDPDWLYLQRLQEEGVKLIGVSGVVNFLPWLRHLPANVRNIRFLLEGKAKTHAIYDRIVEACGQRLKEKQKVFKELQEQKRLQRQLEKEQLRQSKEADPSQEQSEADEDDEESDEEDTYEPECILEHFLAVRDTDSQLYCDDQLRHLLADLFGAGVDTSLATLRWFLLYLAREQRCQRRLHELLLPLGPSPTLEELEPLAYLRACISETMRIRSVVPLGIPHGCKENFVVGDYFIKGGSMIVCSEWAIHMDPVAFPEPEEFRPERFLTADGAYQAPPQFIPFSSGYRMCPGEEMARMILTLFTGRILRRFHLELPSGTEVDMAGESGITLTPTPHMLRFTKLPAVEMRHAPDGAVVQD

Target ORF information:

RefSeq Version	NM_133091.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phantom (phm), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133091.3

ORF Insert Sequence:

ATGTCGGCGG ACATCGTCGA TATTGGCCAC ACCGGTTGGA TGCCCTCGGT GCAGAGCCTG 
AGTATTCTGC TGGTTCCGGG TGCGCTCGTC CTGGTGATTC TCTACCTGTG CGAGCGCCAG 
TGCAATGACC TCATGGGTGC CCCACCGCCG GGTCCCTGGG GCCTGCCCTT TCTGGGTTAC 
CTGCCCTTCC TGGACGCCCG TGCGCCGCAC AAGTCACTCC AGAAGCTGGC CAAGCGGTAT 
GGTGGAATTT TCGAGCTGAA AATGGGCAGG GTGCCGACCG TAGTCCTCTC GGATGCCGCC 
TTGGTGCGGG ATTTCTTTCG GCGCGATGTG ATGACTGGCC GTGCGCCGCT CTACCTCACC 
CACGGCATCA TGGGTGGATT TGGCATCATC TGCGCCCAGG AGGACATTTG GCGACATGCA 
CGGCGCGAGA CTATCGATTG GCTAAAGGCC TTGGGCATGA CCCGTCGGCC GGGGGAACTG 
CGCGCGCGGC TGGAGCGGCG CATAGCCCGC GGAGTCGACG AGTGCGTACG GCTTTTCGAT 
ACTGAGGCAA AGAAGAGCTG TGCGTCGGAA GTGAATCCGC TGCCGGCGCT CCATCACTCG 
CTGGGCAACA TAATCAACGA CCTGGTCTTC GGGATCACCT ACAAGCGCGA CGACCCCGAC 
TGGCTGTACC TGCAGCGGCT GCAGGAGGAG GGCGTCAAGC TGATTGGCGT CTCCGGGGTG 
GTCAACTTTT TGCCCTGGCT GCGTCACCTG CCCGCCAACG TGCGCAACAT CCGCTTCCTG 
CTGGAGGGCA AGGCCAAGAC GCACGCCATC TACGACCGCA TTGTGGAGGC CTGTGGCCAG 
CGGCTGAAGG AGAAGCAGAA GGTGTTCAAG GAGCTCCAGG AGCAGAAGCG GCTGCAAAGG 
CAGCTAGAGA AGGAGCAGCT CAGGCAGTCA AAGGAAGCGG ATCCAAGCCA GGAGCAGAGT 
GAGGCAGACG AGGATGACGA GGAGAGCGAT GAGGAGGACA CGTACGAGCC GGAGTGCATC 
CTGGAGCACT TCCTAGCCGT TCGAGACACG GATTCGCAGC TCTACTGCGA CGACCAGCTG 
CGCCATCTGC TGGCCGATCT CTTTGGAGCC GGGGTGGACA CCTCGCTGGC CACCCTGCGC 
TGGTTCCTGC TCTACTTGGC CCGCGAACAA CGCTGCCAGC GGCGCCTGCA TGAGCTCCTC 
CTGCCGCTGG GTCCGTCTCC CACTTTGGAG GAACTGGAGC CGCTGGCCTA CCTAAGGGCT 
TGCATTTCCG AGACGATGCG CATACGCAGC GTTGTCCCAC TGGGCATTCC GCACGGATGC 
AAAGAGAACT TCGTCGTGGG CGATTATTTT ATCAAGGGTG GTTCGATGAT CGTTTGCTCG 
GAGTGGGCTA TCCACATGGA CCCAGTGGCC TTCCCGGAAC CGGAGGAGTT CCGTCCGGAG 
CGCTTCTTGA CCGCCGATGG AGCCTACCAG GCGCCGCCAC AGTTCATCCC ATTCTCGTCC 
GGCTATCGAA TGTGTCCCGG CGAAGAGATG GCTCGCATGA TACTCACGCT CTTTACGGGT 
CGCATCCTCA GGCGCTTCCA CTTGGAACTG CCCTCGGGCA CTGAGGTGGA CATGGCGGGT 
GAGAGCGGCA TCACCCTGAC CCCCACTCCG CACATGCTGC GATTCACCAA GCTGCCGGCG 
GTGGAGATGC GCCATGCACC CGACGGAGCT GTGGTGCAGG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phm ( NM_001298485.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285414.1 Drosophila melanogaster phantom (phm), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa02048
Clone ID Related Accession (Same CDS sequence)	NM_133091.3 , NM_001298485.1
Accession Version	NM_001298485.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phantom, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGCGG ACATCGTCGA TATTGGCCAC ACCGGTTGGA TGCCCTCGGT GCAGAGCCTG 
AGTATTCTGC TGGTTCCGGG TGCGCTCGTC CTGGTGATTC TCTACCTGTG CGAGCGCCAG 
TGCAATGACC TCATGGGTGC CCCACCGCCG GGTCCCTGGG GCCTGCCCTT TCTGGGTTAC 
CTGCCCTTCC TGGACGCCCG TGCGCCGCAC AAGTCACTCC AGAAGCTGGC CAAGCGGTAT 
GGTGGAATTT TCGAGCTGAA AATGGGCAGG GTGCCGACCG TAGTCCTCTC GGATGCCGCC 
TTGGTGCGGG ATTTCTTTCG GCGCGATGTG ATGACTGGCC GTGCGCCGCT CTACCTCACC 
CACGGCATCA TGGGTGGATT TGGCATCATC TGCGCCCAGG AGGACATTTG GCGACATGCA 
CGGCGCGAGA CTATCGATTG GCTAAAGGCC TTGGGCATGA CCCGTCGGCC GGGGGAACTG 
CGCGCGCGGC TGGAGCGGCG CATAGCCCGC GGAGTCGACG AGTGCGTACG GCTTTTCGAT 
ACTGAGGCAA AGAAGAGCTG TGCGTCGGAA GTGAATCCGC TGCCGGCGCT CCATCACTCG 
CTGGGCAACA TAATCAACGA CCTGGTCTTC GGGATCACCT ACAAGCGCGA CGACCCCGAC 
TGGCTGTACC TGCAGCGGCT GCAGGAGGAG GGCGTCAAGC TGATTGGCGT CTCCGGGGTG 
GTCAACTTTT TGCCCTGGCT GCGTCACCTG CCCGCCAACG TGCGCAACAT CCGCTTCCTG 
CTGGAGGGCA AGGCCAAGAC GCACGCCATC TACGACCGCA TTGTGGAGGC CTGTGGCCAG 
CGGCTGAAGG AGAAGCAGAA GGTGTTCAAG GAGCTCCAGG AGCAGAAGCG GCTGCAAAGG 
CAGCTAGAGA AGGAGCAGCT CAGGCAGTCA AAGGAAGCGG ATCCAAGCCA GGAGCAGAGT 
GAGGCAGACG AGGATGACGA GGAGAGCGAT GAGGAGGACA CGTACGAGCC GGAGTGCATC 
CTGGAGCACT TCCTAGCCGT TCGAGACACG GATTCGCAGC TCTACTGCGA CGACCAGCTG 
CGCCATCTGC TGGCCGATCT CTTTGGAGCC GGGGTGGACA CCTCGCTGGC CACCCTGCGC 
TGGTTCCTGC TCTACTTGGC CCGCGAACAA CGCTGCCAGC GGCGCCTGCA TGAGCTCCTC 
CTGCCGCTGG GTCCGTCTCC CACTTTGGAG GAACTGGAGC CGCTGGCCTA CCTAAGGGCT 
TGCATTTCCG AGACGATGCG CATACGCAGC GTTGTCCCAC TGGGCATTCC GCACGGATGC 
AAAGAGAACT TCGTCGTGGG CGATTATTTT ATCAAGGGTG GTTCGATGAT CGTTTGCTCG 
GAGTGGGCTA TCCACATGGA CCCAGTGGCC TTCCCGGAAC CGGAGGAGTT CCGTCCGGAG 
CGCTTCTTGA CCGCCGATGG AGCCTACCAG GCGCCGCCAC AGTTCATCCC ATTCTCGTCC 
GGCTATCGAA TGTGTCCCGG CGAAGAGATG GCTCGCATGA TACTCACGCT CTTTACGGGT 
CGCATCCTCA GGCGCTTCCA CTTGGAACTG CCCTCGGGCA CTGAGGTGGA CATGGCGGGT 
GAGAGCGGCA TCACCCTGAC CCCCACTCCG CACATGCTGC GATTCACCAA GCTGCCGGCG 
GTGGAGATGC GCCATGCACC CGACGGAGCT GTGGTGCAGG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285414.1
CDS	197..1921
Translation	MSADIVDIGHTGWMPSVQSLSILLVPGALVLVILYLCERQCNDLMGAPPPGPWGLPFLGYLPFLDARAPHKSLQKLAKRYGGIFELKMGRVPTVVLSDAALVRDFFRRDVMTGRAPLYLTHGIMGGFGIICAQEDIWRHARRETIDWLKALGMTRRPGELRARLERRIARGVDECVRLFDTEAKKSCASEVNPLPALHHSLGNIINDLVFGITYKRDDPDWLYLQRLQEEGVKLIGVSGVVNFLPWLRHLPANVRNIRFLLEGKAKTHAIYDRIVEACGQRLKEKQKVFKELQEQKRLQRQLEKEQLRQSKEADPSQEQSEADEDDEESDEEDTYEPECILEHFLAVRDTDSQLYCDDQLRHLLADLFGAGVDTSLATLRWFLLYLAREQRCQRRLHELLLPLGPSPTLEELEPLAYLRACISETMRIRSVVPLGIPHGCKENFVVGDYFIKGGSMIVCSEWAIHMDPVAFPEPEEFRPERFLTADGAYQAPPQFIPFSSGYRMCPGEEMARMILTLFTGRILRRFHLELPSGTEVDMAGESGITLTPTPHMLRFTKLPAVEMRHAPDGAVVQD

Target ORF information:

RefSeq Version	NM_001298485.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phantom (phm), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298485.1

ORF Insert Sequence:

ATGTCGGCGG ACATCGTCGA TATTGGCCAC ACCGGTTGGA TGCCCTCGGT GCAGAGCCTG 
AGTATTCTGC TGGTTCCGGG TGCGCTCGTC CTGGTGATTC TCTACCTGTG CGAGCGCCAG 
TGCAATGACC TCATGGGTGC CCCACCGCCG GGTCCCTGGG GCCTGCCCTT TCTGGGTTAC 
CTGCCCTTCC TGGACGCCCG TGCGCCGCAC AAGTCACTCC AGAAGCTGGC CAAGCGGTAT 
GGTGGAATTT TCGAGCTGAA AATGGGCAGG GTGCCGACCG TAGTCCTCTC GGATGCCGCC 
TTGGTGCGGG ATTTCTTTCG GCGCGATGTG ATGACTGGCC GTGCGCCGCT CTACCTCACC 
CACGGCATCA TGGGTGGATT TGGCATCATC TGCGCCCAGG AGGACATTTG GCGACATGCA 
CGGCGCGAGA CTATCGATTG GCTAAAGGCC TTGGGCATGA CCCGTCGGCC GGGGGAACTG 
CGCGCGCGGC TGGAGCGGCG CATAGCCCGC GGAGTCGACG AGTGCGTACG GCTTTTCGAT 
ACTGAGGCAA AGAAGAGCTG TGCGTCGGAA GTGAATCCGC TGCCGGCGCT CCATCACTCG 
CTGGGCAACA TAATCAACGA CCTGGTCTTC GGGATCACCT ACAAGCGCGA CGACCCCGAC 
TGGCTGTACC TGCAGCGGCT GCAGGAGGAG GGCGTCAAGC TGATTGGCGT CTCCGGGGTG 
GTCAACTTTT TGCCCTGGCT GCGTCACCTG CCCGCCAACG TGCGCAACAT CCGCTTCCTG 
CTGGAGGGCA AGGCCAAGAC GCACGCCATC TACGACCGCA TTGTGGAGGC CTGTGGCCAG 
CGGCTGAAGG AGAAGCAGAA GGTGTTCAAG GAGCTCCAGG AGCAGAAGCG GCTGCAAAGG 
CAGCTAGAGA AGGAGCAGCT CAGGCAGTCA AAGGAAGCGG ATCCAAGCCA GGAGCAGAGT 
GAGGCAGACG AGGATGACGA GGAGAGCGAT GAGGAGGACA CGTACGAGCC GGAGTGCATC 
CTGGAGCACT TCCTAGCCGT TCGAGACACG GATTCGCAGC TCTACTGCGA CGACCAGCTG 
CGCCATCTGC TGGCCGATCT CTTTGGAGCC GGGGTGGACA CCTCGCTGGC CACCCTGCGC 
TGGTTCCTGC TCTACTTGGC CCGCGAACAA CGCTGCCAGC GGCGCCTGCA TGAGCTCCTC 
CTGCCGCTGG GTCCGTCTCC CACTTTGGAG GAACTGGAGC CGCTGGCCTA CCTAAGGGCT 
TGCATTTCCG AGACGATGCG CATACGCAGC GTTGTCCCAC TGGGCATTCC GCACGGATGC 
AAAGAGAACT TCGTCGTGGG CGATTATTTT ATCAAGGGTG GTTCGATGAT CGTTTGCTCG 
GAGTGGGCTA TCCACATGGA CCCAGTGGCC TTCCCGGAAC CGGAGGAGTT CCGTCCGGAG 
CGCTTCTTGA CCGCCGATGG AGCCTACCAG GCGCCGCCAC AGTTCATCCC ATTCTCGTCC 
GGCTATCGAA TGTGTCCCGG CGAAGAGATG GCTCGCATGA TACTCACGCT CTTTACGGGT 
CGCATCCTCA GGCGCTTCCA CTTGGAACTG CCCTCGGGCA CTGAGGTGGA CATGGCGGGT 
GAGAGCGGCA TCACCCTGAC CCCCACTCCG CACATGCTGC GATTCACCAA GCTGCCGGCG 
GTGGAGATGC GCCATGCACC CGACGGAGCT GTGGTGCAGG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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phm cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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