Wnt5 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Wnt5
Entrez Gene ID	32838
Full Name	Wnt oncogene analog 5
Synonyms	CG6407,DWnt-3,DWnt-3/5,DWnt-5,DWnt3,DWnt3/5,DWnt5,Dm DWnt3/5,Dm-3,Dmel\CG6407,Dwnt-3,Dwnt-5,Dwnt3,Dwnt5,WNT5,Wnt,Wnt-3,Wnt3,Wnt3/5,anon-WO03040301.228,wnt3,wnt5
General protein information	Preferred Names Wnt oncogene analog 5 Names CG6407 gene product from transcript CG6407-RB CG6407-PA CG6407-PB Wnt5-PA Wnt5-PB wnt-oncogene-analog-3
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 17B6-17C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_057576.5	NP_476924.1	Wnt oncogene analog 5, isoform A
NM_001298478.1	NP_001285407.1	Wnt oncogene analog 5, isoform B

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Wnt4	NP_033549.1
Drosophila melanogaster (fruit fly)	Wnt5	NP_476924.1
Caenorhabditis elegans (roundworm)	cwn-1	NP_493668.1
Xenopus tropicalis (tropical clawed frog)	wnt4	NP_001239015.1
Homo sapiens (human)	WNT4	NP_110388.2
Pan troglodytes (chimpanzee)	WNT4	XP_524597.3
Bos taurus (cattle)	WNT4	XP_003585839.2
Rattus norvegicus (Norway rat)	Wnt4	NP_445854.1
Canis lupus familiaris (dog)	WNT4	XP_855190.3
Gallus gallus (chicken)	WNT4	NP_990114.1
Danio rerio (zebrafish)	wnt4a	NP_001035477.1
	WNT4	XP_001100814.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The pool of ADP-ribosylated Axin, which is degraded under basal conditions, increases immediately following Wnt stimulation in both Drosophila and human cells.
Title: Wnt pathway activation by ADP-ribosylation.

Wnt5 promotes dendrite termination on the periphery of sternites through the Ryk receptor family kinase Derailed (Drl) and the Rho GTPase guanine nucleotide exchange factor Trio in C4da neurons
Title: Adult Drosophila sensory neurons specify dendritic territories independently of dendritic contacts through the Wnt5-Drl signaling pathway.

Wnt5 is required for projection neurons dendrites migration and targeting in the developing antennal lobe.
Title: Wnt5 and drl/ryk gradients pattern the Drosophila olfactory dendritic map.

WNT5 interacts with doughnut and derailed to mediate muscle attachment site selection
Title: WNT5 interacts with the Ryk receptors doughnut and derailed to mediate muscle attachment site selection in Drosophila melanogaster.

Wnt5 is broadly expressed in the developing brain and regulates axonal development of mushroom body neurons.
Title: The Wnt5/planar cell polarity pathway regulates axonal development of the Drosophila mushroom body neuron.

The following Wnt5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Wnt5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa02027	NM_057576.5 Latest version!	Drosophila melanogaster Wnt oncogene analog 5 (Wnt5), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa02027	NM_001298478.1 Latest version!	Drosophila melanogaster Wnt oncogene analog 5 (Wnt5), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Wnt5 ( NM_057576.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476924.1 Drosophila melanogaster Wnt oncogene analog 5 (Wnt5), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa02027
Clone ID Related Accession (Same CDS sequence)	NM_057576.5 , NM_001298478.1
Accession Version	NM_057576.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3015bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Wnt oncogene analog 5, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_057576.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTTGCT ACAGAAAAAG GCACTTTCTA TTGTGGCTCT TGCGTGCTGT GTGTATGTTG 
CACTTAACCG CGAGAGGGGC ATATGCCACA GTTGGGTTGC AAGGAGTGCC GACATGGATA 
TATCTCGGCC TCAAGTCCCC CTTCATCGAG TTTGGCAACC AGGTGGAGCA GCTGGCCAAT 
TCCAGCATAC CACTGAACAT GACCAAGGAC GAGCAGGCCA ATATGCATCA AGAGGGCCTA 
CGCAAGCTCG GTACGTTCAT AAAGCCAGTG GACCTGCGGG ACTCGGAGAC TGGCTTCGTC 
AAGGCCGATC TCACCAAGAG ACTGGTATTC GATAGACCGA ACAACATTAC ATCTCGCCCT 
ATTCACCCGA TACAGGAGGA GATGGATCAG AAGCAGATAA TCCTGCTCGA CGAGGATACC 
GACGAGAATG GCCTGCCAGC CAGTCTCACC GACGAGGATC GCAAGTTTAT AGTGCCGATG 
GCGCTCAAGA ATATATCGCC CGATCCACGC TGGGCGGCCA CTACACCGAG TCCCTCCGCT 
TTGCAGCCGA ACGCTAAAGC CATCTCGACC ATTGTGCCCT CGCCTCTGGC CCAGGTCGAG 
GGGGATCCCA CGTCCAACAT CGATGACCTG AAGAAGCACA TACTCTTCTT GCACAACATG 
ACCAAGACCA ATTCGAACTT CGAGTCGAAA TTCGTTAAAT TCCCGAGCCT GCAAAAGGAC 
AAGGCCAAGA CATCGGGAGC TGGCGGTTCG CCGCCCAATC CCAAGCGGCC CCAGCGGCCG 
ATTCATCAGT ATTCCGCGCC CATAGCCCCA CCAACACCCA AGGTGCCCGC GCCAGATGGC 
GGCGGCGTAG GAGGAGCAGC TTACAATCCC GGAGAGCAGC CAATTGGTGG CTACTATCAG 
AACGAGGAAC TAGCGAATAA TCAATCCCTT CTTAAACCAA CAGATACCGA CTCCCATCCA 
GCGGCCGGCG GTAGCAGCCA TGGCCAGAAG AATCCCAGCG AGCCCCAGGT GATACTGCTC 
AACGAGACAC TCTCCACGGA GACCTCAATC GAAGCGGATC GCAGTCCATC GATAAACCAG 
CCCAAGGCGG GATCGCCTGC GCGCACAACA AAGCGACCAC CTTGCCTGCG CAATCCCGAG 
TCCCCGAAAT GCATACGTCA GCGTCGGCGG GAGGAGCAAC AGCGGCAGCG GGAGCGGGAC 
GAGTGGTTCC GCGGTCAGTC GCAGTACATG CAGCCCCGGT TCGAGCCGAT CATACAGACG 
ATTAACAATA CGAAGAGATT TGCCGTATCA ATCGAGATTC CAGACTCCTT TAAAGTATCC 
TCCGAGGGAT CGGATGGGGA GTTGCTTTCG CGAGTCGAAC GCTCGCAGCC CAGCATTAGT 
AGTAGTAGTA GTAGCAGTAG TAGCAGTAGT AGGAAAATCA TGCCAGACTA TATTAAGGTA 
TCCATGGAGA ACAACACATC CGTCACGGAT TATTTTAAGC ACGACGTTGT GATGACATCG 
GCAGATGTCG CCAGCGATAG GGAATTCCTT ATCAAGAACA TGGAGGAGCA CGGAGGCGCT 
GGCTCCGCGA ACAGTCATCA CAATGATACG ACTCCAACTG CAGACGCATA TTCGGAGACA 
ATCGATCTTA ATCCCAATAA CTGCTATAGC GCAATAGGTC TAAGCAACAG CCAAAAGAAG 
CAATGTGTTA AGCACACCAG CGTGATGCCG GCCATAAGTC GTGGTGCCCG TGCCGCCATC 
CAGGAGTGCC AGTTTCAGTT CAAGAATCGC CGCTGGAACT GCAGCACAAC GAACGACGAG 
ACCGTATTTG GTCCCATGAC CAGCCTGGCT GCTCCCGAAA TGGCCTTCAT CCACGCCCTG 
GCCGCGGCCA CGGTGACCAG CTTCATAGCT CGCGCCTGCC GGGATGGCCA ACTGGCCTCC 
TGCAGCTGCT CCCGCGGCAG TCGACCCAAA CAGCTCCACG ACGACTGGAA GTGGGGCGGC 
TGTGGCGACA ACCTGGAGTT CGCCTACAAG TTCGCCACGG ACTTCATCGA TTCGCGGGAG 
AAGGAAACCA ATCGCGAGAC GCGTGGCGTT AAGAGAAAAC GCGAGGAGAT CAACAAGAAT 
CGCATGCATT CCGATGACAC GAATGCTTTT AACATAGGTA TTAAACGTAA CAAAAACGTA 
GATGCTAAAA ACGATACAAG TTTGGTAGTG AGAAACGTTA GGAAAAGCAC TGAGGCTGAA 
AACAGTCACA TACTCAATGA GAACTTTGAT CAGCACCTAT TGGAACTAGA GCAGCGCATT 
ACGAAGGAGA TACTTACATC CAAGATAGAC GAGGAGGAGA TGATTAAGCT GCAGGAGAAG 
ATCAAACAGG AGATTGTCAA CACCAAGTTC TTCAAGGGTG AGCAGCAGCC GCGCAAGAAG 
AAGCGAAAAA ACCAGAGAGC CGCCGCCGAT GCGCCCGCCT ATCCGAGGAA CGGCATCAAG 
GAGAGCTACA AGGATGGCGG CATATTGCCG CGCAGCACGG CCACTGTCAA GGCCAGGAGC 
CTGATGAACT TGCACAACAA CGAGGCCGGA CGTCGGGCGG TGATCAAGAA GGCCAGGATA 
ACGTGCAAGT GCCACGGCGT GTCCGGCTCC TGCAGCCTGA TCACCTGCTG GCAGCAATTG 
TCCTCCATCC GGGAGATTGG CGACTATCTG CGCGAGAAGT ACGAGGGCGC CACCAAGGTG 
AAGATCAACA AGCGTGGCCG CCTCCAGATC AAGGACTTGC AATTCAAGGT GCCGACCGCT 
CACGATCTTA TTTACCTAGA CGAAAGTCCC GACTGGTGCC GCAATAGCTA TGCGCTGCAT 
TGGCCGGGAA CGCACGGACG TGTGTGCCAC AAAAACTCGT CGGGATTGGA GAGCTGTGCC 
ATCCTCTGCT GCGGCCGGGG CTATAATACG AAGAACATTA TAGTTAACGA ACGCTGCAAT 
TGCAAATTTC ACTGGTGTTG CCAGGTTAAA TGTGAAGTTT GTACGAAGGT ACTCGAGGAG 
CACACATGTA AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476924.1
CDS	554..3568
Translation	MSCYRKRHFLLWLLRAVCMLHLTARGAYATVGLQGVPTWIYLGLKSPFIEFGNQVEQLANSSIPLNMTKDEQANMHQEGLRKLGTFIKPVDLRDSETGFVKADLTKRLVFDRPNNITSRPIHPIQEEMDQKQIILLDEDTDENGLPASLTDEDRKFIVPMALKNISPDPRWAATTPSPSALQPNAKAISTIVPSPLAQVEGDPTSNIDDLKKHILFLHNMTKTNSNFESKFVKFPSLQKDKAKTSGAGGSPPNPKRPQRPIHQYSAPIAPPTPKVPAPDGGGVGGAAYNPGEQPIGGYYQNEELANNQSLLKPTDTDSHPAAGGSSHGQKNPSEPQVILLNETLSTETSIEADRSPSINQPKAGSPARTTKRPPCLRNPESPKCIRQRRREEQQRQRERDEWFRGQSQYMQPRFEPIIQTINNTKRFAVSIEIPDSFKVSSEGSDGELLSRVERSQPSISSSSSSSSSSSRKIMPDYIKVSMENNTSVTDYFKHDVVMTSADVASDREFLIKNMEEHGGAGSANSHHNDTTPTADAYSETIDLNPNNCYSAIGLSNSQKKQCVKHTSVMPAISRGARAAIQECQFQFKNRRWNCSTTNDETVFGPMTSLAAPEMAFIHALAAATVTSFIARACRDGQLASCSCSRGSRPKQLHDDWKWGGCGDNLEFAYKFATDFIDSREKETNRETRGVKRKREEINKNRMHSDDTNAFNIGIKRNKNVDAKNDTSLVVRNVRKSTEAENSHILNENFDQHLLELEQRITKEILTSKIDEEEMIKLQEKIKQEIVNTKFFKGEQQPRKKKRKNQRAAADAPAYPRNGIKESYKDGGILPRSTATVKARSLMNLHNNEAGRRAVIKKARITCKCHGVSGSCSLITCWQQLSSIREIGDYLREKYEGATKVKINKRGRLQIKDLQFKVPTAHDLIYLDESPDWCRNSYALHWPGTHGRVCHKNSSGLESCAILCCGRGYNTKNIIVNERCNCKFHWCCQVKCEVCTKVLEEHTCK

Target ORF information:

RefSeq Version	NM_057576.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Wnt oncogene analog 5 (Wnt5), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057576.5

ORF Insert Sequence:

ATGAGTTGCT ACAGAAAAAG GCACTTTCTA TTGTGGCTCT TGCGTGCTGT GTGTATGTTG 
CACTTAACCG CGAGAGGGGC ATATGCCACA GTTGGGTTGC AAGGAGTGCC GACATGGATA 
TATCTCGGCC TCAAGTCCCC CTTCATCGAG TTTGGCAACC AGGTGGAGCA GCTGGCCAAT 
TCCAGCATAC CACTGAACAT GACCAAGGAC GAGCAGGCCA ATATGCATCA AGAGGGCCTA 
CGCAAGCTCG GTACGTTCAT AAAGCCAGTG GACCTGCGGG ACTCGGAGAC TGGCTTCGTC 
AAGGCCGATC TCACCAAGAG ACTGGTATTC GATAGACCGA ACAACATTAC ATCTCGCCCT 
ATTCACCCGA TACAGGAGGA GATGGATCAG AAGCAGATAA TCCTGCTCGA CGAGGATACC 
GACGAGAATG GCCTGCCAGC CAGTCTCACC GACGAGGATC GCAAGTTTAT AGTGCCGATG 
GCGCTCAAGA ATATATCGCC CGATCCACGC TGGGCGGCCA CTACACCGAG TCCCTCCGCT 
TTGCAGCCGA ACGCTAAAGC CATCTCGACC ATTGTGCCCT CGCCTCTGGC CCAGGTCGAG 
GGGGATCCCA CGTCCAACAT CGATGACCTG AAGAAGCACA TACTCTTCTT GCACAACATG 
ACCAAGACCA ATTCGAACTT CGAGTCGAAA TTCGTTAAAT TCCCGAGCCT GCAAAAGGAC 
AAGGCCAAGA CATCGGGAGC TGGCGGTTCG CCGCCCAATC CCAAGCGGCC CCAGCGGCCG 
ATTCATCAGT ATTCCGCGCC CATAGCCCCA CCAACACCCA AGGTGCCCGC GCCAGATGGC 
GGCGGCGTAG GAGGAGCAGC TTACAATCCC GGAGAGCAGC CAATTGGTGG CTACTATCAG 
AACGAGGAAC TAGCGAATAA TCAATCCCTT CTTAAACCAA CAGATACCGA CTCCCATCCA 
GCGGCCGGCG GTAGCAGCCA TGGCCAGAAG AATCCCAGCG AGCCCCAGGT GATACTGCTC 
AACGAGACAC TCTCCACGGA GACCTCAATC GAAGCGGATC GCAGTCCATC GATAAACCAG 
CCCAAGGCGG GATCGCCTGC GCGCACAACA AAGCGACCAC CTTGCCTGCG CAATCCCGAG 
TCCCCGAAAT GCATACGTCA GCGTCGGCGG GAGGAGCAAC AGCGGCAGCG GGAGCGGGAC 
GAGTGGTTCC GCGGTCAGTC GCAGTACATG CAGCCCCGGT TCGAGCCGAT CATACAGACG 
ATTAACAATA CGAAGAGATT TGCCGTATCA ATCGAGATTC CAGACTCCTT TAAAGTATCC 
TCCGAGGGAT CGGATGGGGA GTTGCTTTCG CGAGTCGAAC GCTCGCAGCC CAGCATTAGT 
AGTAGTAGTA GTAGCAGTAG TAGCAGTAGT AGGAAAATCA TGCCAGACTA TATTAAGGTA 
TCCATGGAGA ACAACACATC CGTCACGGAT TATTTTAAGC ACGACGTTGT GATGACATCG 
GCAGATGTCG CCAGCGATAG GGAATTCCTT ATCAAGAACA TGGAGGAGCA CGGAGGCGCT 
GGCTCCGCGA ACAGTCATCA CAATGATACG ACTCCAACTG CAGACGCATA TTCGGAGACA 
ATCGATCTTA ATCCCAATAA CTGCTATAGC GCAATAGGTC TAAGCAACAG CCAAAAGAAG 
CAATGTGTTA AGCACACCAG CGTGATGCCG GCCATAAGTC GTGGTGCCCG TGCCGCCATC 
CAGGAGTGCC AGTTTCAGTT CAAGAATCGC CGCTGGAACT GCAGCACAAC GAACGACGAG 
ACCGTATTTG GTCCCATGAC CAGCCTGGCT GCTCCCGAAA TGGCCTTCAT CCACGCCCTG 
GCCGCGGCCA CGGTGACCAG CTTCATAGCT CGCGCCTGCC GGGATGGCCA ACTGGCCTCC 
TGCAGCTGCT CCCGCGGCAG TCGACCCAAA CAGCTCCACG ACGACTGGAA GTGGGGCGGC 
TGTGGCGACA ACCTGGAGTT CGCCTACAAG TTCGCCACGG ACTTCATCGA TTCGCGGGAG 
AAGGAAACCA ATCGCGAGAC GCGTGGCGTT AAGAGAAAAC GCGAGGAGAT CAACAAGAAT 
CGCATGCATT CCGATGACAC GAATGCTTTT AACATAGGTA TTAAACGTAA CAAAAACGTA 
GATGCTAAAA ACGATACAAG TTTGGTAGTG AGAAACGTTA GGAAAAGCAC TGAGGCTGAA 
AACAGTCACA TACTCAATGA GAACTTTGAT CAGCACCTAT TGGAACTAGA GCAGCGCATT 
ACGAAGGAGA TACTTACATC CAAGATAGAC GAGGAGGAGA TGATTAAGCT GCAGGAGAAG 
ATCAAACAGG AGATTGTCAA CACCAAGTTC TTCAAGGGTG AGCAGCAGCC GCGCAAGAAG 
AAGCGAAAAA ACCAGAGAGC CGCCGCCGAT GCGCCCGCCT ATCCGAGGAA CGGCATCAAG 
GAGAGCTACA AGGATGGCGG CATATTGCCG CGCAGCACGG CCACTGTCAA GGCCAGGAGC 
CTGATGAACT TGCACAACAA CGAGGCCGGA CGTCGGGCGG TGATCAAGAA GGCCAGGATA 
ACGTGCAAGT GCCACGGCGT GTCCGGCTCC TGCAGCCTGA TCACCTGCTG GCAGCAATTG 
TCCTCCATCC GGGAGATTGG CGACTATCTG CGCGAGAAGT ACGAGGGCGC CACCAAGGTG 
AAGATCAACA AGCGTGGCCG CCTCCAGATC AAGGACTTGC AATTCAAGGT GCCGACCGCT 
CACGATCTTA TTTACCTAGA CGAAAGTCCC GACTGGTGCC GCAATAGCTA TGCGCTGCAT 
TGGCCGGGAA CGCACGGACG TGTGTGCCAC AAAAACTCGT CGGGATTGGA GAGCTGTGCC 
ATCCTCTGCT GCGGCCGGGG CTATAATACG AAGAACATTA TAGTTAACGA ACGCTGCAAT 
TGCAAATTTC ACTGGTGTTG CCAGGTTAAA TGTGAAGTTT GTACGAAGGT ACTCGAGGAG 
CACACATGTA AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wnt5 ( NM_001298478.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285407.1 Drosophila melanogaster Wnt oncogene analog 5 (Wnt5), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa02027
Clone ID Related Accession (Same CDS sequence)	NM_057576.5 , NM_001298478.1
Accession Version	NM_001298478.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3015bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Wnt oncogene analog 5, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTTGCT ACAGAAAAAG GCACTTTCTA TTGTGGCTCT TGCGTGCTGT GTGTATGTTG 
CACTTAACCG CGAGAGGGGC ATATGCCACA GTTGGGTTGC AAGGAGTGCC GACATGGATA 
TATCTCGGCC TCAAGTCCCC CTTCATCGAG TTTGGCAACC AGGTGGAGCA GCTGGCCAAT 
TCCAGCATAC CACTGAACAT GACCAAGGAC GAGCAGGCCA ATATGCATCA AGAGGGCCTA 
CGCAAGCTCG GTACGTTCAT AAAGCCAGTG GACCTGCGGG ACTCGGAGAC TGGCTTCGTC 
AAGGCCGATC TCACCAAGAG ACTGGTATTC GATAGACCGA ACAACATTAC ATCTCGCCCT 
ATTCACCCGA TACAGGAGGA GATGGATCAG AAGCAGATAA TCCTGCTCGA CGAGGATACC 
GACGAGAATG GCCTGCCAGC CAGTCTCACC GACGAGGATC GCAAGTTTAT AGTGCCGATG 
GCGCTCAAGA ATATATCGCC CGATCCACGC TGGGCGGCCA CTACACCGAG TCCCTCCGCT 
TTGCAGCCGA ACGCTAAAGC CATCTCGACC ATTGTGCCCT CGCCTCTGGC CCAGGTCGAG 
GGGGATCCCA CGTCCAACAT CGATGACCTG AAGAAGCACA TACTCTTCTT GCACAACATG 
ACCAAGACCA ATTCGAACTT CGAGTCGAAA TTCGTTAAAT TCCCGAGCCT GCAAAAGGAC 
AAGGCCAAGA CATCGGGAGC TGGCGGTTCG CCGCCCAATC CCAAGCGGCC CCAGCGGCCG 
ATTCATCAGT ATTCCGCGCC CATAGCCCCA CCAACACCCA AGGTGCCCGC GCCAGATGGC 
GGCGGCGTAG GAGGAGCAGC TTACAATCCC GGAGAGCAGC CAATTGGTGG CTACTATCAG 
AACGAGGAAC TAGCGAATAA TCAATCCCTT CTTAAACCAA CAGATACCGA CTCCCATCCA 
GCGGCCGGCG GTAGCAGCCA TGGCCAGAAG AATCCCAGCG AGCCCCAGGT GATACTGCTC 
AACGAGACAC TCTCCACGGA GACCTCAATC GAAGCGGATC GCAGTCCATC GATAAACCAG 
CCCAAGGCGG GATCGCCTGC GCGCACAACA AAGCGACCAC CTTGCCTGCG CAATCCCGAG 
TCCCCGAAAT GCATACGTCA GCGTCGGCGG GAGGAGCAAC AGCGGCAGCG GGAGCGGGAC 
GAGTGGTTCC GCGGTCAGTC GCAGTACATG CAGCCCCGGT TCGAGCCGAT CATACAGACG 
ATTAACAATA CGAAGAGATT TGCCGTATCA ATCGAGATTC CAGACTCCTT TAAAGTATCC 
TCCGAGGGAT CGGATGGGGA GTTGCTTTCG CGAGTCGAAC GCTCGCAGCC CAGCATTAGT 
AGTAGTAGTA GTAGCAGTAG TAGCAGTAGT AGGAAAATCA TGCCAGACTA TATTAAGGTA 
TCCATGGAGA ACAACACATC CGTCACGGAT TATTTTAAGC ACGACGTTGT GATGACATCG 
GCAGATGTCG CCAGCGATAG GGAATTCCTT ATCAAGAACA TGGAGGAGCA CGGAGGCGCT 
GGCTCCGCGA ACAGTCATCA CAATGATACG ACTCCAACTG CAGACGCATA TTCGGAGACA 
ATCGATCTTA ATCCCAATAA CTGCTATAGC GCAATAGGTC TAAGCAACAG CCAAAAGAAG 
CAATGTGTTA AGCACACCAG CGTGATGCCG GCCATAAGTC GTGGTGCCCG TGCCGCCATC 
CAGGAGTGCC AGTTTCAGTT CAAGAATCGC CGCTGGAACT GCAGCACAAC GAACGACGAG 
ACCGTATTTG GTCCCATGAC CAGCCTGGCT GCTCCCGAAA TGGCCTTCAT CCACGCCCTG 
GCCGCGGCCA CGGTGACCAG CTTCATAGCT CGCGCCTGCC GGGATGGCCA ACTGGCCTCC 
TGCAGCTGCT CCCGCGGCAG TCGACCCAAA CAGCTCCACG ACGACTGGAA GTGGGGCGGC 
TGTGGCGACA ACCTGGAGTT CGCCTACAAG TTCGCCACGG ACTTCATCGA TTCGCGGGAG 
AAGGAAACCA ATCGCGAGAC GCGTGGCGTT AAGAGAAAAC GCGAGGAGAT CAACAAGAAT 
CGCATGCATT CCGATGACAC GAATGCTTTT AACATAGGTA TTAAACGTAA CAAAAACGTA 
GATGCTAAAA ACGATACAAG TTTGGTAGTG AGAAACGTTA GGAAAAGCAC TGAGGCTGAA 
AACAGTCACA TACTCAATGA GAACTTTGAT CAGCACCTAT TGGAACTAGA GCAGCGCATT 
ACGAAGGAGA TACTTACATC CAAGATAGAC GAGGAGGAGA TGATTAAGCT GCAGGAGAAG 
ATCAAACAGG AGATTGTCAA CACCAAGTTC TTCAAGGGTG AGCAGCAGCC GCGCAAGAAG 
AAGCGAAAAA ACCAGAGAGC CGCCGCCGAT GCGCCCGCCT ATCCGAGGAA CGGCATCAAG 
GAGAGCTACA AGGATGGCGG CATATTGCCG CGCAGCACGG CCACTGTCAA GGCCAGGAGC 
CTGATGAACT TGCACAACAA CGAGGCCGGA CGTCGGGCGG TGATCAAGAA GGCCAGGATA 
ACGTGCAAGT GCCACGGCGT GTCCGGCTCC TGCAGCCTGA TCACCTGCTG GCAGCAATTG 
TCCTCCATCC GGGAGATTGG CGACTATCTG CGCGAGAAGT ACGAGGGCGC CACCAAGGTG 
AAGATCAACA AGCGTGGCCG CCTCCAGATC AAGGACTTGC AATTCAAGGT GCCGACCGCT 
CACGATCTTA TTTACCTAGA CGAAAGTCCC GACTGGTGCC GCAATAGCTA TGCGCTGCAT 
TGGCCGGGAA CGCACGGACG TGTGTGCCAC AAAAACTCGT CGGGATTGGA GAGCTGTGCC 
ATCCTCTGCT GCGGCCGGGG CTATAATACG AAGAACATTA TAGTTAACGA ACGCTGCAAT 
TGCAAATTTC ACTGGTGTTG CCAGGTTAAA TGTGAAGTTT GTACGAAGGT ACTCGAGGAG 
CACACATGTA AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285407.1
CDS	554..3568
Translation	MSCYRKRHFLLWLLRAVCMLHLTARGAYATVGLQGVPTWIYLGLKSPFIEFGNQVEQLANSSIPLNMTKDEQANMHQEGLRKLGTFIKPVDLRDSETGFVKADLTKRLVFDRPNNITSRPIHPIQEEMDQKQIILLDEDTDENGLPASLTDEDRKFIVPMALKNISPDPRWAATTPSPSALQPNAKAISTIVPSPLAQVEGDPTSNIDDLKKHILFLHNMTKTNSNFESKFVKFPSLQKDKAKTSGAGGSPPNPKRPQRPIHQYSAPIAPPTPKVPAPDGGGVGGAAYNPGEQPIGGYYQNEELANNQSLLKPTDTDSHPAAGGSSHGQKNPSEPQVILLNETLSTETSIEADRSPSINQPKAGSPARTTKRPPCLRNPESPKCIRQRRREEQQRQRERDEWFRGQSQYMQPRFEPIIQTINNTKRFAVSIEIPDSFKVSSEGSDGELLSRVERSQPSISSSSSSSSSSSRKIMPDYIKVSMENNTSVTDYFKHDVVMTSADVASDREFLIKNMEEHGGAGSANSHHNDTTPTADAYSETIDLNPNNCYSAIGLSNSQKKQCVKHTSVMPAISRGARAAIQECQFQFKNRRWNCSTTNDETVFGPMTSLAAPEMAFIHALAAATVTSFIARACRDGQLASCSCSRGSRPKQLHDDWKWGGCGDNLEFAYKFATDFIDSREKETNRETRGVKRKREEINKNRMHSDDTNAFNIGIKRNKNVDAKNDTSLVVRNVRKSTEAENSHILNENFDQHLLELEQRITKEILTSKIDEEEMIKLQEKIKQEIVNTKFFKGEQQPRKKKRKNQRAAADAPAYPRNGIKESYKDGGILPRSTATVKARSLMNLHNNEAGRRAVIKKARITCKCHGVSGSCSLITCWQQLSSIREIGDYLREKYEGATKVKINKRGRLQIKDLQFKVPTAHDLIYLDESPDWCRNSYALHWPGTHGRVCHKNSSGLESCAILCCGRGYNTKNIIVNERCNCKFHWCCQVKCEVCTKVLEEHTCK

Target ORF information:

RefSeq Version	NM_001298478.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Wnt oncogene analog 5 (Wnt5), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298478.1

ORF Insert Sequence:

ATGAGTTGCT ACAGAAAAAG GCACTTTCTA TTGTGGCTCT TGCGTGCTGT GTGTATGTTG 
CACTTAACCG CGAGAGGGGC ATATGCCACA GTTGGGTTGC AAGGAGTGCC GACATGGATA 
TATCTCGGCC TCAAGTCCCC CTTCATCGAG TTTGGCAACC AGGTGGAGCA GCTGGCCAAT 
TCCAGCATAC CACTGAACAT GACCAAGGAC GAGCAGGCCA ATATGCATCA AGAGGGCCTA 
CGCAAGCTCG GTACGTTCAT AAAGCCAGTG GACCTGCGGG ACTCGGAGAC TGGCTTCGTC 
AAGGCCGATC TCACCAAGAG ACTGGTATTC GATAGACCGA ACAACATTAC ATCTCGCCCT 
ATTCACCCGA TACAGGAGGA GATGGATCAG AAGCAGATAA TCCTGCTCGA CGAGGATACC 
GACGAGAATG GCCTGCCAGC CAGTCTCACC GACGAGGATC GCAAGTTTAT AGTGCCGATG 
GCGCTCAAGA ATATATCGCC CGATCCACGC TGGGCGGCCA CTACACCGAG TCCCTCCGCT 
TTGCAGCCGA ACGCTAAAGC CATCTCGACC ATTGTGCCCT CGCCTCTGGC CCAGGTCGAG 
GGGGATCCCA CGTCCAACAT CGATGACCTG AAGAAGCACA TACTCTTCTT GCACAACATG 
ACCAAGACCA ATTCGAACTT CGAGTCGAAA TTCGTTAAAT TCCCGAGCCT GCAAAAGGAC 
AAGGCCAAGA CATCGGGAGC TGGCGGTTCG CCGCCCAATC CCAAGCGGCC CCAGCGGCCG 
ATTCATCAGT ATTCCGCGCC CATAGCCCCA CCAACACCCA AGGTGCCCGC GCCAGATGGC 
GGCGGCGTAG GAGGAGCAGC TTACAATCCC GGAGAGCAGC CAATTGGTGG CTACTATCAG 
AACGAGGAAC TAGCGAATAA TCAATCCCTT CTTAAACCAA CAGATACCGA CTCCCATCCA 
GCGGCCGGCG GTAGCAGCCA TGGCCAGAAG AATCCCAGCG AGCCCCAGGT GATACTGCTC 
AACGAGACAC TCTCCACGGA GACCTCAATC GAAGCGGATC GCAGTCCATC GATAAACCAG 
CCCAAGGCGG GATCGCCTGC GCGCACAACA AAGCGACCAC CTTGCCTGCG CAATCCCGAG 
TCCCCGAAAT GCATACGTCA GCGTCGGCGG GAGGAGCAAC AGCGGCAGCG GGAGCGGGAC 
GAGTGGTTCC GCGGTCAGTC GCAGTACATG CAGCCCCGGT TCGAGCCGAT CATACAGACG 
ATTAACAATA CGAAGAGATT TGCCGTATCA ATCGAGATTC CAGACTCCTT TAAAGTATCC 
TCCGAGGGAT CGGATGGGGA GTTGCTTTCG CGAGTCGAAC GCTCGCAGCC CAGCATTAGT 
AGTAGTAGTA GTAGCAGTAG TAGCAGTAGT AGGAAAATCA TGCCAGACTA TATTAAGGTA 
TCCATGGAGA ACAACACATC CGTCACGGAT TATTTTAAGC ACGACGTTGT GATGACATCG 
GCAGATGTCG CCAGCGATAG GGAATTCCTT ATCAAGAACA TGGAGGAGCA CGGAGGCGCT 
GGCTCCGCGA ACAGTCATCA CAATGATACG ACTCCAACTG CAGACGCATA TTCGGAGACA 
ATCGATCTTA ATCCCAATAA CTGCTATAGC GCAATAGGTC TAAGCAACAG CCAAAAGAAG 
CAATGTGTTA AGCACACCAG CGTGATGCCG GCCATAAGTC GTGGTGCCCG TGCCGCCATC 
CAGGAGTGCC AGTTTCAGTT CAAGAATCGC CGCTGGAACT GCAGCACAAC GAACGACGAG 
ACCGTATTTG GTCCCATGAC CAGCCTGGCT GCTCCCGAAA TGGCCTTCAT CCACGCCCTG 
GCCGCGGCCA CGGTGACCAG CTTCATAGCT CGCGCCTGCC GGGATGGCCA ACTGGCCTCC 
TGCAGCTGCT CCCGCGGCAG TCGACCCAAA CAGCTCCACG ACGACTGGAA GTGGGGCGGC 
TGTGGCGACA ACCTGGAGTT CGCCTACAAG TTCGCCACGG ACTTCATCGA TTCGCGGGAG 
AAGGAAACCA ATCGCGAGAC GCGTGGCGTT AAGAGAAAAC GCGAGGAGAT CAACAAGAAT 
CGCATGCATT CCGATGACAC GAATGCTTTT AACATAGGTA TTAAACGTAA CAAAAACGTA 
GATGCTAAAA ACGATACAAG TTTGGTAGTG AGAAACGTTA GGAAAAGCAC TGAGGCTGAA 
AACAGTCACA TACTCAATGA GAACTTTGAT CAGCACCTAT TGGAACTAGA GCAGCGCATT 
ACGAAGGAGA TACTTACATC CAAGATAGAC GAGGAGGAGA TGATTAAGCT GCAGGAGAAG 
ATCAAACAGG AGATTGTCAA CACCAAGTTC TTCAAGGGTG AGCAGCAGCC GCGCAAGAAG 
AAGCGAAAAA ACCAGAGAGC CGCCGCCGAT GCGCCCGCCT ATCCGAGGAA CGGCATCAAG 
GAGAGCTACA AGGATGGCGG CATATTGCCG CGCAGCACGG CCACTGTCAA GGCCAGGAGC 
CTGATGAACT TGCACAACAA CGAGGCCGGA CGTCGGGCGG TGATCAAGAA GGCCAGGATA 
ACGTGCAAGT GCCACGGCGT GTCCGGCTCC TGCAGCCTGA TCACCTGCTG GCAGCAATTG 
TCCTCCATCC GGGAGATTGG CGACTATCTG CGCGAGAAGT ACGAGGGCGC CACCAAGGTG 
AAGATCAACA AGCGTGGCCG CCTCCAGATC AAGGACTTGC AATTCAAGGT GCCGACCGCT 
CACGATCTTA TTTACCTAGA CGAAAGTCCC GACTGGTGCC GCAATAGCTA TGCGCTGCAT 
TGGCCGGGAA CGCACGGACG TGTGTGCCAC AAAAACTCGT CGGGATTGGA GAGCTGTGCC 
ATCCTCTGCT GCGGCCGGGG CTATAATACG AAGAACATTA TAGTTAACGA ACGCTGCAAT 
TGCAAATTTC ACTGGTGTTG CCAGGTTAAA TGTGAAGTTT GTACGAAGGT ACTCGAGGAG 
CACACATGTA AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Wnt5 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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