upd1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	upd1
Entrez Gene ID	32813
Full Name	unpaired 1
Synonyms	CG5993,Dmel\CG5993,OS,Os,UPD,Upd,Upd1,l(1)YC43,l(1)YM55,od,odsy,os,sis-C,sis-c,sisC,sy,unp,upa,upd
General protein information	Preferred Names unpaired 1 Names CG5993 gene product from transcript CG5993-RC CG5993-PA CG5993-PB CG5993-PC Unpaired/Out-stretched outstretched outstretched small eye outstretched-smalleye sisterless c small-eye unpaired upd homolog upd1-PA upd1-PB upd1-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 17A5-17A5

mRNA and Protein(s)

mRNA	Protein	Name
NM_080356.4	NP_525095.2	unpaired 1, isoform A
NM_001103545.3	NP_001097015.1	unpaired 1, isoform B
NM_001298473.1	NP_001285402.1	unpaired 1, isoform C

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

a mechanism based on the dilution of the growth modulator Upd can explain how growth termination is controlled in the eye disc
Title: Growth control in the

adipose- and brain-derived upd/leptin may control differing features of weight regulation through distinct neural circuits.
Title: A Leptin Analog Locally Produced in the Brain Acts via a Conserved Neural Circuit to Modulate Obesity-Linked Behaviors in Drosophila.

ROS/JNK/p38/Upd stress responsive module restores tissue homeostasis. This module is not only activated after cell death induction but also after physical damage and reveals one of the earliest responses for imaginal disc regeneration.
Title: ROS-Induced JNK and p38 Signaling Is Required for Unpaired Cytokine Activation during Drosophila Regeneration.

in the Drosophila egg chamber, two anterior cells secrete Unpaired to activate Signal transducer and activator of transcription (STAT) signalling in the epithelium.
Title: Tissue landscape alters adjacent cell fates during Drosophila egg development.

data suggest that Dally and Dlp are essential regulators for Upd distribution and JAK/STAT signaling activity in eye development
Title: Drosophila glypicans Dally and Dally-like are essential regulators for JAK/STAT signaling and Unpaired distribution in eye development.

The following upd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the upd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01995	NM_001103545.3 Latest version!	Drosophila melanogaster unpaired 1 (upd1), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa01995	NM_080356.4 Latest version!	Drosophila melanogaster unpaired 1 (upd1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa01995	NM_001298473.1 Latest version!	Drosophila melanogaster unpaired 1 (upd1), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

upd1 ( NM_080356.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_525095.2 Drosophila melanogaster unpaired 1 (upd1), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa01995
Clone ID Related Accession (Same CDS sequence)	NM_080356.4 , NM_001103545.3 , NM_001298473.1
Accession Version	NM_080356.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1242bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	unpaired 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_080356.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTCGTC CGCTGCTCCT GCTCCTGCCC ATCCTACTCG TCCTGCTCCT TGCCGCCCAG 
TTGCCCCACC TCGCCGAGGG GCGCAGCACC ACCAGCAGCG GCGGTCTAAC GGTTATCGAT 
AGCGAGCGCC TGCGCATTCG CACCACCTCC AGTACGGCTT CAGCTCAGCA TCCCAATCAG 
GGCACGATCC CTGCCAGCGC CGCTAGTCCG CGCAAGCGCC ACAGAAAGCG GAACAGCAAC 
TGGATCGACT ATCGCAACTT CGACGAGAAC ACCACCGCCC TGGAGTGGGC CAATCCCTGC 
GGTGGCAACT ATCATCCCTC GGCGGGTGAT CGCTTCAATC GCCAGCGTCC CAGGCAGAGC 
TTCAATCAGC TGAAGCGCCA CGCCTTTCGG GAGTACCGCA GCCTAAACAG TAGCCAGGAC 
TCGGCCATAG ATATCCGCAA CATGACCATG TGGTCCCTCC ACACGCACAA CTACAAGTTC 
CTGCCCAAGC TCAAGCCCAA TTCCACGATT GCCCTAAAGC GCTGGTACCG CAACATGCAA 
ACTTACGTGG CCAGCTTTGC GTATCTGAGG CGCCAGCAGA TCCGCTGGGA TCAGCGGTCC 
ATCACCCGCG AGTCCAGCAC CGCCCGCGAG CTGCGCGAGC TGCTCCTGAG CTCGCGGCGC 
ATCCTCTGCG AGCTGGAGAC AGCCGTCAAC CAGACGCAGA GTCCGCGCCA GAAGCAGCGG 
CGCAGTGGTG CGGCGGTCAC AGCAGTTGGC GGCACCACCA TGCTGGGTCA GCAGCTGCCA 
CAGATCTCGC GTCTCGAGAT GAACAAGCGT TTGAAGCTGC GCTCCAAGAC GAGTGGGCCC 
GGTATGGGTG GTGCTGCTTC CAGTGCATCC ATGGCGGCGG GCGAGGCGGA CTCGATCGAT 
ATGCGCTTTG TGAAGCACCA CTACTACGAC TTTTTGCGCA CCATGTACCA GCTGCTGCGG 
CGGGATGGCA AGCGGGTAAG GAGTCGGCCA CGGAAGCACC ACAAGAAGCA GCAGAGGAGC 
CAGAAAAAGT TGCTACAGCA GCAGCAGCAG CTGGCGGATC AACTGGAGCA GCGTTGGCGC 
AGCTCCACCT TGAATGGCAA GGAGTTCAAC GAGGTATCCA AGCCAGTGGC CGGTGGTGCT 
GCTGGAGTTG CAGATGTTGC AGGTGTTTCC GGTGGTGGAA GCAACCAATT TGCACGTGGC 
AGACGTGGAA AGCGCCAGTC GAAGCGCGTG CAGCGCACGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_525095.2
CDS	661..1902
Translation	MARPLLLLLPILLVLLLAAQLPHLAEGRSTTSSGGLTVIDSERLRIRTTSSTASAQHPNQGTIPASAASPRKRHRKRNSNWIDYRNFDENTTALEWANPCGGNYHPSAGDRFNRQRPRQSFNQLKRHAFREYRSLNSSQDSAIDIRNMTMWSLHTHNYKFLPKLKPNSTIALKRWYRNMQTYVASFAYLRRQQIRWDQRSITRESSTARELRELLLSSRRILCELETAVNQTQSPRQKQRRSGAAVTAVGGTTMLGQQLPQISRLEMNKRLKLRSKTSGPGMGGAASSASMAAGEADSIDMRFVKHHYYDFLRTMYQLLRRDGKRVRSRPRKHHKKQQRSQKKLLQQQQQLADQLEQRWRSSTLNGKEFNEVSKPVAGGAAGVADVAGVSGGGSNQFARGRRGKRQSKRVQRT

Target ORF information:

RefSeq Version	NM_080356.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster unpaired 1 (upd1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080356.4

ORF Insert Sequence:

ATGGCTCGTC CGCTGCTCCT GCTCCTGCCC ATCCTACTCG TCCTGCTCCT TGCCGCCCAG 
TTGCCCCACC TCGCCGAGGG GCGCAGCACC ACCAGCAGCG GCGGTCTAAC GGTTATCGAT 
AGCGAGCGCC TGCGCATTCG CACCACCTCC AGTACGGCTT CAGCTCAGCA TCCCAATCAG 
GGCACGATCC CTGCCAGCGC CGCTAGTCCG CGCAAGCGCC ACAGAAAGCG GAACAGCAAC 
TGGATCGACT ATCGCAACTT CGACGAGAAC ACCACCGCCC TGGAGTGGGC CAATCCCTGC 
GGTGGCAACT ATCATCCCTC GGCGGGTGAT CGCTTCAATC GCCAGCGTCC CAGGCAGAGC 
TTCAATCAGC TGAAGCGCCA CGCCTTTCGG GAGTACCGCA GCCTAAACAG TAGCCAGGAC 
TCGGCCATAG ATATCCGCAA CATGACCATG TGGTCCCTCC ACACGCACAA CTACAAGTTC 
CTGCCCAAGC TCAAGCCCAA TTCCACGATT GCCCTAAAGC GCTGGTACCG CAACATGCAA 
ACTTACGTGG CCAGCTTTGC GTATCTGAGG CGCCAGCAGA TCCGCTGGGA TCAGCGGTCC 
ATCACCCGCG AGTCCAGCAC CGCCCGCGAG CTGCGCGAGC TGCTCCTGAG CTCGCGGCGC 
ATCCTCTGCG AGCTGGAGAC AGCCGTCAAC CAGACGCAGA GTCCGCGCCA GAAGCAGCGG 
CGCAGTGGTG CGGCGGTCAC AGCAGTTGGC GGCACCACCA TGCTGGGTCA GCAGCTGCCA 
CAGATCTCGC GTCTCGAGAT GAACAAGCGT TTGAAGCTGC GCTCCAAGAC GAGTGGGCCC 
GGTATGGGTG GTGCTGCTTC CAGTGCATCC ATGGCGGCGG GCGAGGCGGA CTCGATCGAT 
ATGCGCTTTG TGAAGCACCA CTACTACGAC TTTTTGCGCA CCATGTACCA GCTGCTGCGG 
CGGGATGGCA AGCGGGTAAG GAGTCGGCCA CGGAAGCACC ACAAGAAGCA GCAGAGGAGC 
CAGAAAAAGT TGCTACAGCA GCAGCAGCAG CTGGCGGATC AACTGGAGCA GCGTTGGCGC 
AGCTCCACCT TGAATGGCAA GGAGTTCAAC GAGGTATCCA AGCCAGTGGC CGGTGGTGCT 
GCTGGAGTTG CAGATGTTGC AGGTGTTTCC GGTGGTGGAA GCAACCAATT TGCACGTGGC 
AGACGTGGAA AGCGCCAGTC GAAGCGCGTG CAGCGCACGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

upd1 ( NM_001103545.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097015.1 Drosophila melanogaster unpaired 1 (upd1), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa01995
Clone ID Related Accession (Same CDS sequence)	NM_080356.4 , NM_001103545.3 , NM_001298473.1
Accession Version	NM_001103545.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1242bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	unpaired 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001103545.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTCGTC CGCTGCTCCT GCTCCTGCCC ATCCTACTCG TCCTGCTCCT TGCCGCCCAG 
TTGCCCCACC TCGCCGAGGG GCGCAGCACC ACCAGCAGCG GCGGTCTAAC GGTTATCGAT 
AGCGAGCGCC TGCGCATTCG CACCACCTCC AGTACGGCTT CAGCTCAGCA TCCCAATCAG 
GGCACGATCC CTGCCAGCGC CGCTAGTCCG CGCAAGCGCC ACAGAAAGCG GAACAGCAAC 
TGGATCGACT ATCGCAACTT CGACGAGAAC ACCACCGCCC TGGAGTGGGC CAATCCCTGC 
GGTGGCAACT ATCATCCCTC GGCGGGTGAT CGCTTCAATC GCCAGCGTCC CAGGCAGAGC 
TTCAATCAGC TGAAGCGCCA CGCCTTTCGG GAGTACCGCA GCCTAAACAG TAGCCAGGAC 
TCGGCCATAG ATATCCGCAA CATGACCATG TGGTCCCTCC ACACGCACAA CTACAAGTTC 
CTGCCCAAGC TCAAGCCCAA TTCCACGATT GCCCTAAAGC GCTGGTACCG CAACATGCAA 
ACTTACGTGG CCAGCTTTGC GTATCTGAGG CGCCAGCAGA TCCGCTGGGA TCAGCGGTCC 
ATCACCCGCG AGTCCAGCAC CGCCCGCGAG CTGCGCGAGC TGCTCCTGAG CTCGCGGCGC 
ATCCTCTGCG AGCTGGAGAC AGCCGTCAAC CAGACGCAGA GTCCGCGCCA GAAGCAGCGG 
CGCAGTGGTG CGGCGGTCAC AGCAGTTGGC GGCACCACCA TGCTGGGTCA GCAGCTGCCA 
CAGATCTCGC GTCTCGAGAT GAACAAGCGT TTGAAGCTGC GCTCCAAGAC GAGTGGGCCC 
GGTATGGGTG GTGCTGCTTC CAGTGCATCC ATGGCGGCGG GCGAGGCGGA CTCGATCGAT 
ATGCGCTTTG TGAAGCACCA CTACTACGAC TTTTTGCGCA CCATGTACCA GCTGCTGCGG 
CGGGATGGCA AGCGGGTAAG GAGTCGGCCA CGGAAGCACC ACAAGAAGCA GCAGAGGAGC 
CAGAAAAAGT TGCTACAGCA GCAGCAGCAG CTGGCGGATC AACTGGAGCA GCGTTGGCGC 
AGCTCCACCT TGAATGGCAA GGAGTTCAAC GAGGTATCCA AGCCAGTGGC CGGTGGTGCT 
GCTGGAGTTG CAGATGTTGC AGGTGTTTCC GGTGGTGGAA GCAACCAATT TGCACGTGGC 
AGACGTGGAA AGCGCCAGTC GAAGCGCGTG CAGCGCACGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097015.1
CDS	462..1703
Translation	MARPLLLLLPILLVLLLAAQLPHLAEGRSTTSSGGLTVIDSERLRIRTTSSTASAQHPNQGTIPASAASPRKRHRKRNSNWIDYRNFDENTTALEWANPCGGNYHPSAGDRFNRQRPRQSFNQLKRHAFREYRSLNSSQDSAIDIRNMTMWSLHTHNYKFLPKLKPNSTIALKRWYRNMQTYVASFAYLRRQQIRWDQRSITRESSTARELRELLLSSRRILCELETAVNQTQSPRQKQRRSGAAVTAVGGTTMLGQQLPQISRLEMNKRLKLRSKTSGPGMGGAASSASMAAGEADSIDMRFVKHHYYDFLRTMYQLLRRDGKRVRSRPRKHHKKQQRSQKKLLQQQQQLADQLEQRWRSSTLNGKEFNEVSKPVAGGAAGVADVAGVSGGGSNQFARGRRGKRQSKRVQRT

Target ORF information:

RefSeq Version	NM_001103545.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster unpaired 1 (upd1), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103545.3

ORF Insert Sequence:

ATGGCTCGTC CGCTGCTCCT GCTCCTGCCC ATCCTACTCG TCCTGCTCCT TGCCGCCCAG 
TTGCCCCACC TCGCCGAGGG GCGCAGCACC ACCAGCAGCG GCGGTCTAAC GGTTATCGAT 
AGCGAGCGCC TGCGCATTCG CACCACCTCC AGTACGGCTT CAGCTCAGCA TCCCAATCAG 
GGCACGATCC CTGCCAGCGC CGCTAGTCCG CGCAAGCGCC ACAGAAAGCG GAACAGCAAC 
TGGATCGACT ATCGCAACTT CGACGAGAAC ACCACCGCCC TGGAGTGGGC CAATCCCTGC 
GGTGGCAACT ATCATCCCTC GGCGGGTGAT CGCTTCAATC GCCAGCGTCC CAGGCAGAGC 
TTCAATCAGC TGAAGCGCCA CGCCTTTCGG GAGTACCGCA GCCTAAACAG TAGCCAGGAC 
TCGGCCATAG ATATCCGCAA CATGACCATG TGGTCCCTCC ACACGCACAA CTACAAGTTC 
CTGCCCAAGC TCAAGCCCAA TTCCACGATT GCCCTAAAGC GCTGGTACCG CAACATGCAA 
ACTTACGTGG CCAGCTTTGC GTATCTGAGG CGCCAGCAGA TCCGCTGGGA TCAGCGGTCC 
ATCACCCGCG AGTCCAGCAC CGCCCGCGAG CTGCGCGAGC TGCTCCTGAG CTCGCGGCGC 
ATCCTCTGCG AGCTGGAGAC AGCCGTCAAC CAGACGCAGA GTCCGCGCCA GAAGCAGCGG 
CGCAGTGGTG CGGCGGTCAC AGCAGTTGGC GGCACCACCA TGCTGGGTCA GCAGCTGCCA 
CAGATCTCGC GTCTCGAGAT GAACAAGCGT TTGAAGCTGC GCTCCAAGAC GAGTGGGCCC 
GGTATGGGTG GTGCTGCTTC CAGTGCATCC ATGGCGGCGG GCGAGGCGGA CTCGATCGAT 
ATGCGCTTTG TGAAGCACCA CTACTACGAC TTTTTGCGCA CCATGTACCA GCTGCTGCGG 
CGGGATGGCA AGCGGGTAAG GAGTCGGCCA CGGAAGCACC ACAAGAAGCA GCAGAGGAGC 
CAGAAAAAGT TGCTACAGCA GCAGCAGCAG CTGGCGGATC AACTGGAGCA GCGTTGGCGC 
AGCTCCACCT TGAATGGCAA GGAGTTCAAC GAGGTATCCA AGCCAGTGGC CGGTGGTGCT 
GCTGGAGTTG CAGATGTTGC AGGTGTTTCC GGTGGTGGAA GCAACCAATT TGCACGTGGC 
AGACGTGGAA AGCGCCAGTC GAAGCGCGTG CAGCGCACGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

upd1 ( NM_001298473.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285402.1 Drosophila melanogaster unpaired 1 (upd1), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa01995
Clone ID Related Accession (Same CDS sequence)	NM_080356.4 , NM_001103545.3 , NM_001298473.1
Accession Version	NM_001298473.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1242bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	unpaired 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTCGTC CGCTGCTCCT GCTCCTGCCC ATCCTACTCG TCCTGCTCCT TGCCGCCCAG 
TTGCCCCACC TCGCCGAGGG GCGCAGCACC ACCAGCAGCG GCGGTCTAAC GGTTATCGAT 
AGCGAGCGCC TGCGCATTCG CACCACCTCC AGTACGGCTT CAGCTCAGCA TCCCAATCAG 
GGCACGATCC CTGCCAGCGC CGCTAGTCCG CGCAAGCGCC ACAGAAAGCG GAACAGCAAC 
TGGATCGACT ATCGCAACTT CGACGAGAAC ACCACCGCCC TGGAGTGGGC CAATCCCTGC 
GGTGGCAACT ATCATCCCTC GGCGGGTGAT CGCTTCAATC GCCAGCGTCC CAGGCAGAGC 
TTCAATCAGC TGAAGCGCCA CGCCTTTCGG GAGTACCGCA GCCTAAACAG TAGCCAGGAC 
TCGGCCATAG ATATCCGCAA CATGACCATG TGGTCCCTCC ACACGCACAA CTACAAGTTC 
CTGCCCAAGC TCAAGCCCAA TTCCACGATT GCCCTAAAGC GCTGGTACCG CAACATGCAA 
ACTTACGTGG CCAGCTTTGC GTATCTGAGG CGCCAGCAGA TCCGCTGGGA TCAGCGGTCC 
ATCACCCGCG AGTCCAGCAC CGCCCGCGAG CTGCGCGAGC TGCTCCTGAG CTCGCGGCGC 
ATCCTCTGCG AGCTGGAGAC AGCCGTCAAC CAGACGCAGA GTCCGCGCCA GAAGCAGCGG 
CGCAGTGGTG CGGCGGTCAC AGCAGTTGGC GGCACCACCA TGCTGGGTCA GCAGCTGCCA 
CAGATCTCGC GTCTCGAGAT GAACAAGCGT TTGAAGCTGC GCTCCAAGAC GAGTGGGCCC 
GGTATGGGTG GTGCTGCTTC CAGTGCATCC ATGGCGGCGG GCGAGGCGGA CTCGATCGAT 
ATGCGCTTTG TGAAGCACCA CTACTACGAC TTTTTGCGCA CCATGTACCA GCTGCTGCGG 
CGGGATGGCA AGCGGGTAAG GAGTCGGCCA CGGAAGCACC ACAAGAAGCA GCAGAGGAGC 
CAGAAAAAGT TGCTACAGCA GCAGCAGCAG CTGGCGGATC AACTGGAGCA GCGTTGGCGC 
AGCTCCACCT TGAATGGCAA GGAGTTCAAC GAGGTATCCA AGCCAGTGGC CGGTGGTGCT 
GCTGGAGTTG CAGATGTTGC AGGTGTTTCC GGTGGTGGAA GCAACCAATT TGCACGTGGC 
AGACGTGGAA AGCGCCAGTC GAAGCGCGTG CAGCGCACGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285402.1
CDS	621..1862
Translation	MARPLLLLLPILLVLLLAAQLPHLAEGRSTTSSGGLTVIDSERLRIRTTSSTASAQHPNQGTIPASAASPRKRHRKRNSNWIDYRNFDENTTALEWANPCGGNYHPSAGDRFNRQRPRQSFNQLKRHAFREYRSLNSSQDSAIDIRNMTMWSLHTHNYKFLPKLKPNSTIALKRWYRNMQTYVASFAYLRRQQIRWDQRSITRESSTARELRELLLSSRRILCELETAVNQTQSPRQKQRRSGAAVTAVGGTTMLGQQLPQISRLEMNKRLKLRSKTSGPGMGGAASSASMAAGEADSIDMRFVKHHYYDFLRTMYQLLRRDGKRVRSRPRKHHKKQQRSQKKLLQQQQQLADQLEQRWRSSTLNGKEFNEVSKPVAGGAAGVADVAGVSGGGSNQFARGRRGKRQSKRVQRT

Target ORF information:

RefSeq Version	NM_001298473.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster unpaired 1 (upd1), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298473.1

ORF Insert Sequence:

ATGGCTCGTC CGCTGCTCCT GCTCCTGCCC ATCCTACTCG TCCTGCTCCT TGCCGCCCAG 
TTGCCCCACC TCGCCGAGGG GCGCAGCACC ACCAGCAGCG GCGGTCTAAC GGTTATCGAT 
AGCGAGCGCC TGCGCATTCG CACCACCTCC AGTACGGCTT CAGCTCAGCA TCCCAATCAG 
GGCACGATCC CTGCCAGCGC CGCTAGTCCG CGCAAGCGCC ACAGAAAGCG GAACAGCAAC 
TGGATCGACT ATCGCAACTT CGACGAGAAC ACCACCGCCC TGGAGTGGGC CAATCCCTGC 
GGTGGCAACT ATCATCCCTC GGCGGGTGAT CGCTTCAATC GCCAGCGTCC CAGGCAGAGC 
TTCAATCAGC TGAAGCGCCA CGCCTTTCGG GAGTACCGCA GCCTAAACAG TAGCCAGGAC 
TCGGCCATAG ATATCCGCAA CATGACCATG TGGTCCCTCC ACACGCACAA CTACAAGTTC 
CTGCCCAAGC TCAAGCCCAA TTCCACGATT GCCCTAAAGC GCTGGTACCG CAACATGCAA 
ACTTACGTGG CCAGCTTTGC GTATCTGAGG CGCCAGCAGA TCCGCTGGGA TCAGCGGTCC 
ATCACCCGCG AGTCCAGCAC CGCCCGCGAG CTGCGCGAGC TGCTCCTGAG CTCGCGGCGC 
ATCCTCTGCG AGCTGGAGAC AGCCGTCAAC CAGACGCAGA GTCCGCGCCA GAAGCAGCGG 
CGCAGTGGTG CGGCGGTCAC AGCAGTTGGC GGCACCACCA TGCTGGGTCA GCAGCTGCCA 
CAGATCTCGC GTCTCGAGAT GAACAAGCGT TTGAAGCTGC GCTCCAAGAC GAGTGGGCCC 
GGTATGGGTG GTGCTGCTTC CAGTGCATCC ATGGCGGCGG GCGAGGCGGA CTCGATCGAT 
ATGCGCTTTG TGAAGCACCA CTACTACGAC TTTTTGCGCA CCATGTACCA GCTGCTGCGG 
CGGGATGGCA AGCGGGTAAG GAGTCGGCCA CGGAAGCACC ACAAGAAGCA GCAGAGGAGC 
CAGAAAAAGT TGCTACAGCA GCAGCAGCAG CTGGCGGATC AACTGGAGCA GCGTTGGCGC 
AGCTCCACCT TGAATGGCAA GGAGTTCAAC GAGGTATCCA AGCCAGTGGC CGGTGGTGCT 
GCTGGAGTTG CAGATGTTGC AGGTGTTTCC GGTGGTGGAA GCAACCAATT TGCACGTGGC 
AGACGTGGAA AGCGCCAGTC GAAGCGCGTG CAGCGCACGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
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upd1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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