Frq1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Frq1
Entrez Gene ID	32797
Full Name	Frequenin 1
Synonyms	CG5744,Dmel\CG5744,Frq,HL-II,frq,frq1
General protein information	Preferred Names Frequenin 1 Names CG5744 gene product from transcript CG5744-RC CG5744-PB CG5744-PC CG5744-PD CG5744-PE Frequenin2 Frq1-PB Frq1-PC Frq1-PD Frq1-PE frequenin frequenin 1 frequentin frequinin
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 16F7-17A1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001038764.2	NP_001033853.1	frequenin 1, isoform C
NM_133043.3	NP_573271.1	frequenin 1, isoform B
NM_001272744.1	NP_001259673.1	frequenin 1, isoform D
NM_001272745.1	NP_001259674.1	frequenin 1, isoform E

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP013126	XP_003437186.1
Neurospora crassa	NCU04379	XP_957456.1
Mus musculus (house mouse)	Ncs1	NP_062655.1
Gallus gallus (chicken)	NCS1	NP_990708.1
Drosophila melanogaster (fruit fly)	Frq1	NP_573271.1
Kluyveromyces lactis	KLLA0E02399g	XP_454056.1
Schizosaccharomyces pombe (fission yeast)	ncs1	NP_592879.1
Danio rerio (zebrafish)	ncs1b	NP_001018350.1
Eremothecium gossypii	AGOS_ACL163W	NP_983241.1
Homo sapiens (human)	NCS1	NP_055101.2
Bos taurus (cattle)	NCS1	XP_005213538.1
Rattus norvegicus (Norway rat)	Ncs1	NP_077342.1
Xenopus tropicalis (tropical clawed frog)	ncs1	NP_988973.1
Canis lupus familiaris (dog)	NCS1	XP_005625293.1
Drosophila melanogaster (fruit fly)	Frq2	NP_001259675.1
Caenorhabditis elegans (roundworm)	ncs-1	NP_508186.1
Saccharomyces cerevisiae (baker's yeast)	FRQ1	NP_010661.3
Magnaporthe oryzae (rice blast fungus)	MGG_01550	XP_003714524.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

[review] Stimulation protocols, sources of calcium and expression patterns (presynaptic vs postsynaptic) of Frq may result in the activation of various protein targets, leading to different synaptic effects.
Title: Multiple roles for frequenin/NCS-1 in synaptic function and development.

Synonymous substitutions have been prevalent in the evolution of the coding region of frq1 and frq2, indicating the presence of strong functional constraints following gene duplication.
Title: Two frequenins in Drosophila: unveiling the evolutionary history of an unusual neuronal calcium sensor (NCS) duplication.

Frq modulates Ca(2+) entry through a functional interaction with the alpha(1) voltage-gated Ca(2+)-channel subunit; this interaction regulates neurotransmission and nerve-terminal growth.
Title: Frequenin/NCS-1 and the Ca2+-channel alpha1-subunit co-regulate synaptic transmission and nerve-terminal growth.

We conclude that Frqs have two distinct functions: one in neurotransmission, regulating the probability of release per synapse, and another in axonal growth and bouton formation.
Title: Chronic and acute alterations in the functional levels of Frequenins 1 and 2 reveal their roles in synaptic transmission and axon terminal morphology.

The following Frq1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Frq1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01986	NM_001272745.1 Latest version!	Drosophila melanogaster frequenin 1, transcript variant E (Frq1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa01986	NM_001272744.1 Latest version!	Drosophila melanogaster frequenin 1, transcript variant D (Frq1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa01986	NM_001038764.2 Latest version!	Drosophila melanogaster frequenin 1, transcript variant C (Frq1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa01986	NM_133043.3 Latest version!	Drosophila melanogaster frequenin 1, transcript variant B (Frq1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Frq1 ( NM_001038764.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001033853.1 Drosophila melanogaster frequenin 1, transcript variant C (Frq1), mRNA.

Price & Availability↑

CloneID	OFa01986
Clone ID Related Accession (Same CDS sequence)	NM_001038764.2 , NM_133043.3 , NM_001272744.1 , NM_001272745.1
Accession Version	NM_001038764.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	frequenin 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_001038764.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGAAAAA AGAGCTCCAA GTTGAAACAG GATACCATCG ATCGCTTAAC AACGGACACA 
TACTTCACAG AGAAAGAAAT ACGTCAATGG CACAAAGGAT TCCTGAAAGA CTGCCCCAAC 
GGCCTGCTCA CGGAGCAAGG CTTCATCAAA ATATACAAAC AATTCTTTCC ACAAGGCGAT 
CCCAGTAAAT TCGCATCGCT CGTCTTTCGC GTCTTCGATG AGAACAATGA TGGCTCCATT 
GAGTTTGAGG AGTTCATACG CGCCTTATCC GTCACATCGA AGGGCAACCT GGACGAAAAA 
CTGCAGTGGG CCTTCCGTCT CTACGATGTG GACAACGATG GTTACATAAC GCGGGAGGAG 
ATGTACAACA TAGTGGATGC GATCTACCAG ATGGTGGGAC AGCAGCCGCA ATCGGAGGAC 
GAGAACACGC CGCAGAAACG AGTGGACAAG ATCTTCGATC AGATGGACAA AAACCACGAT 
GGCAAATTAA CATTAGAAGA ATTTCGTGAG GGTAGTAAAG CTGATCCACG TATAGTTCAG 
GCGTTAAGTT TAGGTGGTGG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001033853.1
CDS	581..1144
Translation	MGKKSSKLKQDTIDRLTTDTYFTEKEIRQWHKGFLKDCPNGLLTEQGFIKIYKQFFPQGDPSKFASLVFRVFDENNDGSIEFEEFIRALSVTSKGNLDEKLQWAFRLYDVDNDGYITREEMYNIVDAIYQMVGQQPQSEDENTPQKRVDKIFDQMDKNHDGKLTLEEFREGSKADPRIVQALSLGGG

Target ORF information:

RefSeq Version	NM_001038764.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster frequenin 1, transcript variant C (Frq1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001038764.2

ORF Insert Sequence:

ATGGGAAAAA AGAGCTCCAA GTTGAAACAG GATACCATCG ATCGCTTAAC AACGGACACA 
TACTTCACAG AGAAAGAAAT ACGTCAATGG CACAAAGGAT TCCTGAAAGA CTGCCCCAAC 
GGCCTGCTCA CGGAGCAAGG CTTCATCAAA ATATACAAAC AATTCTTTCC ACAAGGCGAT 
CCCAGTAAAT TCGCATCGCT CGTCTTTCGC GTCTTCGATG AGAACAATGA TGGCTCCATT 
GAGTTTGAGG AGTTCATACG CGCCTTATCC GTCACATCGA AGGGCAACCT GGACGAAAAA 
CTGCAGTGGG CCTTCCGTCT CTACGATGTG GACAACGATG GTTACATAAC GCGGGAGGAG 
ATGTACAACA TAGTGGATGC GATCTACCAG ATGGTGGGAC AGCAGCCGCA ATCGGAGGAC 
GAGAACACGC CGCAGAAACG AGTGGACAAG ATCTTCGATC AGATGGACAA AAACCACGAT 
GGCAAATTAA CATTAGAAGA ATTTCGTGAG GGTAGTAAAG CTGATCCACG TATAGTTCAG 
GCGTTAAGTT TAGGTGGTGG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Frq1 ( NM_133043.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_573271.1 Drosophila melanogaster frequenin 1, transcript variant B (Frq1), mRNA.

Price & Availability↑

CloneID	OFa01986
Clone ID Related Accession (Same CDS sequence)	NM_001038764.2 , NM_133043.3 , NM_001272744.1 , NM_001272745.1
Accession Version	NM_133043.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	frequenin 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_133043.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGAAAAA AGAGCTCCAA GTTGAAACAG GATACCATCG ATCGCTTAAC AACGGACACA 
TACTTCACAG AGAAAGAAAT ACGTCAATGG CACAAAGGAT TCCTGAAAGA CTGCCCCAAC 
GGCCTGCTCA CGGAGCAAGG CTTCATCAAA ATATACAAAC AATTCTTTCC ACAAGGCGAT 
CCCAGTAAAT TCGCATCGCT CGTCTTTCGC GTCTTCGATG AGAACAATGA TGGCTCCATT 
GAGTTTGAGG AGTTCATACG CGCCTTATCC GTCACATCGA AGGGCAACCT GGACGAAAAA 
CTGCAGTGGG CCTTCCGTCT CTACGATGTG GACAACGATG GTTACATAAC GCGGGAGGAG 
ATGTACAACA TAGTGGATGC GATCTACCAG ATGGTGGGAC AGCAGCCGCA ATCGGAGGAC 
GAGAACACGC CGCAGAAACG AGTGGACAAG ATCTTCGATC AGATGGACAA AAACCACGAT 
GGCAAATTAA CATTAGAAGA ATTTCGTGAG GGTAGTAAAG CTGATCCACG TATAGTTCAG 
GCGTTAAGTT TAGGTGGTGG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_573271.1
CDS	535..1098
Translation	MGKKSSKLKQDTIDRLTTDTYFTEKEIRQWHKGFLKDCPNGLLTEQGFIKIYKQFFPQGDPSKFASLVFRVFDENNDGSIEFEEFIRALSVTSKGNLDEKLQWAFRLYDVDNDGYITREEMYNIVDAIYQMVGQQPQSEDENTPQKRVDKIFDQMDKNHDGKLTLEEFREGSKADPRIVQALSLGGG

Target ORF information:

RefSeq Version	NM_133043.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster frequenin 1, transcript variant B (Frq1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133043.3

ORF Insert Sequence:

ATGGGAAAAA AGAGCTCCAA GTTGAAACAG GATACCATCG ATCGCTTAAC AACGGACACA 
TACTTCACAG AGAAAGAAAT ACGTCAATGG CACAAAGGAT TCCTGAAAGA CTGCCCCAAC 
GGCCTGCTCA CGGAGCAAGG CTTCATCAAA ATATACAAAC AATTCTTTCC ACAAGGCGAT 
CCCAGTAAAT TCGCATCGCT CGTCTTTCGC GTCTTCGATG AGAACAATGA TGGCTCCATT 
GAGTTTGAGG AGTTCATACG CGCCTTATCC GTCACATCGA AGGGCAACCT GGACGAAAAA 
CTGCAGTGGG CCTTCCGTCT CTACGATGTG GACAACGATG GTTACATAAC GCGGGAGGAG 
ATGTACAACA TAGTGGATGC GATCTACCAG ATGGTGGGAC AGCAGCCGCA ATCGGAGGAC 
GAGAACACGC CGCAGAAACG AGTGGACAAG ATCTTCGATC AGATGGACAA AAACCACGAT 
GGCAAATTAA CATTAGAAGA ATTTCGTGAG GGTAGTAAAG CTGATCCACG TATAGTTCAG 
GCGTTAAGTT TAGGTGGTGG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Frq1 ( NM_001272744.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259673.1 Drosophila melanogaster frequenin 1, transcript variant D (Frq1), mRNA.

Price & Availability↑

CloneID	OFa01986
Clone ID Related Accession (Same CDS sequence)	NM_001038764.2 , NM_133043.3 , NM_001272744.1 , NM_001272745.1
Accession Version	NM_001272744.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	frequenin 1, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGAAAAA AGAGCTCCAA GTTGAAACAG GATACCATCG ATCGCTTAAC AACGGACACA 
TACTTCACAG AGAAAGAAAT ACGTCAATGG CACAAAGGAT TCCTGAAAGA CTGCCCCAAC 
GGCCTGCTCA CGGAGCAAGG CTTCATCAAA ATATACAAAC AATTCTTTCC ACAAGGCGAT 
CCCAGTAAAT TCGCATCGCT CGTCTTTCGC GTCTTCGATG AGAACAATGA TGGCTCCATT 
GAGTTTGAGG AGTTCATACG CGCCTTATCC GTCACATCGA AGGGCAACCT GGACGAAAAA 
CTGCAGTGGG CCTTCCGTCT CTACGATGTG GACAACGATG GTTACATAAC GCGGGAGGAG 
ATGTACAACA TAGTGGATGC GATCTACCAG ATGGTGGGAC AGCAGCCGCA ATCGGAGGAC 
GAGAACACGC CGCAGAAACG AGTGGACAAG ATCTTCGATC AGATGGACAA AAACCACGAT 
GGCAAATTAA CATTAGAAGA ATTTCGTGAG GGTAGTAAAG CTGATCCACG TATAGTTCAG 
GCGTTAAGTT TAGGTGGTGG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259673.1
CDS	443..1006
Translation	MGKKSSKLKQDTIDRLTTDTYFTEKEIRQWHKGFLKDCPNGLLTEQGFIKIYKQFFPQGDPSKFASLVFRVFDENNDGSIEFEEFIRALSVTSKGNLDEKLQWAFRLYDVDNDGYITREEMYNIVDAIYQMVGQQPQSEDENTPQKRVDKIFDQMDKNHDGKLTLEEFREGSKADPRIVQALSLGGG

Target ORF information:

RefSeq Version	NM_001272744.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster frequenin 1, transcript variant D (Frq1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272744.1

ORF Insert Sequence:

ATGGGAAAAA AGAGCTCCAA GTTGAAACAG GATACCATCG ATCGCTTAAC AACGGACACA 
TACTTCACAG AGAAAGAAAT ACGTCAATGG CACAAAGGAT TCCTGAAAGA CTGCCCCAAC 
GGCCTGCTCA CGGAGCAAGG CTTCATCAAA ATATACAAAC AATTCTTTCC ACAAGGCGAT 
CCCAGTAAAT TCGCATCGCT CGTCTTTCGC GTCTTCGATG AGAACAATGA TGGCTCCATT 
GAGTTTGAGG AGTTCATACG CGCCTTATCC GTCACATCGA AGGGCAACCT GGACGAAAAA 
CTGCAGTGGG CCTTCCGTCT CTACGATGTG GACAACGATG GTTACATAAC GCGGGAGGAG 
ATGTACAACA TAGTGGATGC GATCTACCAG ATGGTGGGAC AGCAGCCGCA ATCGGAGGAC 
GAGAACACGC CGCAGAAACG AGTGGACAAG ATCTTCGATC AGATGGACAA AAACCACGAT 
GGCAAATTAA CATTAGAAGA ATTTCGTGAG GGTAGTAAAG CTGATCCACG TATAGTTCAG 
GCGTTAAGTT TAGGTGGTGG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Frq1 ( NM_001272745.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259674.1 Drosophila melanogaster frequenin 1, transcript variant E (Frq1), mRNA.

Price & Availability↑

CloneID	OFa01986
Clone ID Related Accession (Same CDS sequence)	NM_001038764.2 , NM_133043.3 , NM_001272744.1 , NM_001272745.1
Accession Version	NM_001272745.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	frequenin 1, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGAAAAA AGAGCTCCAA GTTGAAACAG GATACCATCG ATCGCTTAAC AACGGACACA 
TACTTCACAG AGAAAGAAAT ACGTCAATGG CACAAAGGAT TCCTGAAAGA CTGCCCCAAC 
GGCCTGCTCA CGGAGCAAGG CTTCATCAAA ATATACAAAC AATTCTTTCC ACAAGGCGAT 
CCCAGTAAAT TCGCATCGCT CGTCTTTCGC GTCTTCGATG AGAACAATGA TGGCTCCATT 
GAGTTTGAGG AGTTCATACG CGCCTTATCC GTCACATCGA AGGGCAACCT GGACGAAAAA 
CTGCAGTGGG CCTTCCGTCT CTACGATGTG GACAACGATG GTTACATAAC GCGGGAGGAG 
ATGTACAACA TAGTGGATGC GATCTACCAG ATGGTGGGAC AGCAGCCGCA ATCGGAGGAC 
GAGAACACGC CGCAGAAACG AGTGGACAAG ATCTTCGATC AGATGGACAA AAACCACGAT 
GGCAAATTAA CATTAGAAGA ATTTCGTGAG GGTAGTAAAG CTGATCCACG TATAGTTCAG 
GCGTTAAGTT TAGGTGGTGG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259674.1
CDS	439..1002
Translation	MGKKSSKLKQDTIDRLTTDTYFTEKEIRQWHKGFLKDCPNGLLTEQGFIKIYKQFFPQGDPSKFASLVFRVFDENNDGSIEFEEFIRALSVTSKGNLDEKLQWAFRLYDVDNDGYITREEMYNIVDAIYQMVGQQPQSEDENTPQKRVDKIFDQMDKNHDGKLTLEEFREGSKADPRIVQALSLGGG

Target ORF information:

RefSeq Version	NM_001272745.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster frequenin 1, transcript variant E (Frq1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272745.1

ORF Insert Sequence:

ATGGGAAAAA AGAGCTCCAA GTTGAAACAG GATACCATCG ATCGCTTAAC AACGGACACA 
TACTTCACAG AGAAAGAAAT ACGTCAATGG CACAAAGGAT TCCTGAAAGA CTGCCCCAAC 
GGCCTGCTCA CGGAGCAAGG CTTCATCAAA ATATACAAAC AATTCTTTCC ACAAGGCGAT 
CCCAGTAAAT TCGCATCGCT CGTCTTTCGC GTCTTCGATG AGAACAATGA TGGCTCCATT 
GAGTTTGAGG AGTTCATACG CGCCTTATCC GTCACATCGA AGGGCAACCT GGACGAAAAA 
CTGCAGTGGG CCTTCCGTCT CTACGATGTG GACAACGATG GTTACATAAC GCGGGAGGAG 
ATGTACAACA TAGTGGATGC GATCTACCAG ATGGTGGGAC AGCAGCCGCA ATCGGAGGAC 
GAGAACACGC CGCAGAAACG AGTGGACAAG ATCTTCGATC AGATGGACAA AAACCACGAT 
GGCAAATTAA CATTAGAAGA ATTTCGTGAG GGTAGTAAAG CTGATCCACG TATAGTTCAG 
GCGTTAAGTT TAGGTGGTGG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Frq1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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