CG8915 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG8915
Entrez Gene ID	32715
Full Name	CG8915 gene product from transcript CG8915-RB
Synonyms	Dmel\CG8915,cg8915
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 15F4-15F4

mRNA and Protein(s)

mRNA	Protein	Name
NM_132980.3	NP_573208.1	uncharacterized protein, isoform A
NM_001298440.1	NP_001285369.1	uncharacterized protein, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG8915 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG8915 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa01888	NM_001298440.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG8915), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa01888	NM_132980.3 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG8915), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG8915 ( NM_132980.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_573208.1 Drosophila melanogaster uncharacterized protein, transcript variant A (CG8915), mRNA.

Price & Availability↑

CloneID	OFa01888
Clone ID Related Accession (Same CDS sequence)	NM_132980.3 , NM_001298440.1
Accession Version	NM_132980.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2931bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_132980.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGACA GTGAAGAAGC AGTGAAACGT GGCCGTCGCC AGGGCAGAAA GAAGGCACAA 
CCTATGCCGG AACAGGCGGT AGTGTTGGTG GTCAATGGCC GAGTGACCGG TGGGCCGAAG 
GGAACTGGCA AACAGAAGAC TCGACCGGGC AAGAACGCGA AAGCCGACGC GGGCGACAAG 
CTGAAGTCCG AGGACGAAAA GATGCTCCGC CAACTGGTCG ACGACTTCCT GCAGAGCTGC 
GAGCCAGAGA AACAGCTCCC CGGACTTACA AAAGCCCAGC GCAGCCACGT TCACCGATTG 
GCCCAGCATC GCGGGCTTAA GACGGTGAGC AAGGGTCCAG AGGAGAACCG CGTCCTTTTT 
ATAAGTCGCC CACAGTCGGA TGGCCAACAG CAATACATTC TTTGCAACAC AAAGCTGGAC 
ATTTGTCCGC CGCTCCTAGA CGTTCTGGGA CAAATGGCCG GCAATGTGGA ACGCAGATTG 
ATGAACTCCG TTGGAGACTT AAAGTGCAAC AAACAGCAAT ATAAGGATAT ACGGCTTCCT 
TCCAACTTCG GCCTGGTCGG GCAGAGGCTT ATTCCGCCGC CGCAGAGCAA CCGGAACAGG 
AACATTCAGC ACGAGCGCCG GAGCCTGCCC ATCTACAAGC AGCGGGAGAG CATCCTCAAC 
GTTCTGCAGC GCGATCAGGT TTTGATCATT AAAGGAGCCA CCGGCTCTGG CAAGTCCACC 
CAACTTCCCC AGTACATCCT TGAGTGGGCC GCAGAGCATC GTGCTCCAGT CAGGATCGTG 
GTAAGTCAGC CTCGCCGCAT AGCAGCTATC AGTGTCTCGG AGCGCATTTC CAAGGAGCGC 
GGTGAGGCGC CTGGCACCAC AGTGGGCTAT AATATTCGCA TGAATAGACA ATGCAGCAGC 
AACACGGTTC TAATGCTGAC CACCAGTGGT TGCCTTTTAA GGGCTCTCGC CATGGATAAG 
AAGTCTTTTT TTAAAAACAC CACCCACTTA ATCATTGATG AAGCCCACGA GCGTGATTTG 
GACACAGACT TCCTGCTGCT GGCCACCAAG CTGGAGCTGC AAAAGAATCC GCACCTTCGC 
CTCGTGCTTA TGTCGGCCAC CATGGATCTT GAAGCATTGT CCAACTATTT TGGTGGGGGC 
ACTGTAATGG ATGTGGAGGG GCGCAGCTTC GAGGTGTCGA TCTATCATCT AGAGGACATT 
CTTAGTAAAA CTGGGTACAT GCACCCGAGA ATGGAGAAGT TTTTGGGCAA GCCGACAGGC 
AAAGAAACTC CTAGCGAACT ACTAGCCGCC TATTATGGAG GTAACACAAT CGTACATCCG 
GATATCGACA ATGACTTGAT CGTTTCGCTA TTGGAATTAT TACTGCGCCA AGGTGACGCT 
GGAGCGGTGA TTGTGTACCT GCCAGGCTAT AGCGATATGA CCTCGCTACT GGCCCGCTTG 
GAATCGTCTC TGCCGCGGGA ACAGATTACT ATCATACTGC TTCATAGCCA GGTGGACAAC 
AGCGAGCAAC GCAAGGTCTT CCGCACTTAT CCTGGCGTGC GTTTAAAGAT TATTCTTAGT 
ACCAACATAG GACAAACTTC GATCACCATT CCGGATCTAC TCTATGTCAT CGACACGGGC 
CTCGCCAAGA TGAAGACCTA TGACTCGACC ATAGATGCAT CGCAGTTAAC CCTCACGTGG 
ATCTCGCAGG CGGACGCCAA ACAGCGTGCG GGAAGAGCTG GACGCGTGTG CCATGGCAAT 
TGCTATCGTC TGTATGACAA CGACCGCATG GCCAGAATGA ATCTGTACAC CATCCCAGAG 
ATCATGAGGC GTACCTTGGA CGAGATATGT CTGCTGACCA AGTTGGCAGC TCCAGACAAG 
AAAATAGAAA ATTTTCTAGC TCTGGCTTTG GATACCCCAC CAAAGGATGC CGTAATGCAG 
TCCTGTTCTA GACTGAAGCT ATTGGGCGTG CTCGACGAAC GGGACGAGGT CACGCCGCTG 
GGACATATCG TTGCCGAACT GCCCTTAGGC GTTCAAATCG GCAAATGCCT GGTGTACTCC 
ATTTACTTGC GCTGCCTGGA CAGCATGATC ATAATTGCGG CCTACCACTC GGTGAGGGAT 
CCCTTTGTTC TAAACATAGA GCGCGGCAAG AAATCAGGGC AGCAGATATC CAGAGTTCTT 
TTCGCTGGCG ACGGAATGAG TGATTCGCTG GCGGTCATTA AACTCTACGA GGAGTTCACC 
AACTTAAAGC GCAAGGACAT CGGCGATTTT TGCGAGCGCA ATTTTATTTG CCGTAACGCA 
ATGGAGATGT TCGTCTCGGC GGCGAGTACT CTACGCGACA CCGTGTACCG TATCTTCCGC 
TTTAGCGAGG CCAGTGCTCG TTTGGCCTCG TCCTTCAACA ATGATACGAA TATGATCCGC 
CTGGCCCTGA CTGCAGGACT GTATCCCAAG TTGGCGTACA TGGATCGCGA GAACAAGAAC 
CAGCTGGTGG CTGAAGGAGA TCCATTAGTT CAGGTTTCGA GAAGCTCTTG CCTTCGGGGC 
AAAAAGAAGC AAAAGGATTT GGCCAGCGAG TGGATCCTGT TCGTTGAGAA GACCCGCACT 
GCTGACCAAA TTTCCTCGCT AGAGCATACC ACCTTGGTGA GCGGCTTGAT GGTGGCTCTG 
GCAGGCGGAA AACGGTTCAT AACCGAGGCG CATGGGTCCG AAATGCTGCT GTGCTTGGAC 
TCCTGGATCC GTTTGAAATG CCCAGCTGAA TTTGGACTCC AGCTCCATAA AGTGCGAATG 
CTAACGGATC GTGAATTCGC CAAGTTGGTA GCGACGCGGA AGCTTAGTCT AATGAGCGAT 
TTTATAGGGC CGGAAACAGT TCGGCGGTTG TTGGAGGCGG ATGTGCCCTC GACTTGCCTG 
GCCGAGGGCA AAAGCCTTCT GGACAGAGAC AACTGTGATA ACGACTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_573208.1
CDS	91..3021
Translation	MADSEEAVKRGRRQGRKKAQPMPEQAVVLVVNGRVTGGPKGTGKQKTRPGKNAKADAGDKLKSEDEKMLRQLVDDFLQSCEPEKQLPGLTKAQRSHVHRLAQHRGLKTVSKGPEENRVLFISRPQSDGQQQYILCNTKLDICPPLLDVLGQMAGNVERRLMNSVGDLKCNKQQYKDIRLPSNFGLVGQRLIPPPQSNRNRNIQHERRSLPIYKQRESILNVLQRDQVLIIKGATGSGKSTQLPQYILEWAAEHRAPVRIVVSQPRRIAAISVSERISKERGEAPGTTVGYNIRMNRQCSSNTVLMLTTSGCLLRALAMDKKSFFKNTTHLIIDEAHERDLDTDFLLLATKLELQKNPHLRLVLMSATMDLEALSNYFGGGTVMDVEGRSFEVSIYHLEDILSKTGYMHPRMEKFLGKPTGKETPSELLAAYYGGNTIVHPDIDNDLIVSLLELLLRQGDAGAVIVYLPGYSDMTSLLARLESSLPREQITIILLHSQVDNSEQRKVFRTYPGVRLKIILSTNIGQTSITIPDLLYVIDTGLAKMKTYDSTIDASQLTLTWISQADAKQRAGRAGRVCHGNCYRLYDNDRMARMNLYTIPEIMRRTLDEICLLTKLAAPDKKIENFLALALDTPPKDAVMQSCSRLKLLGVLDERDEVTPLGHIVAELPLGVQIGKCLVYSIYLRCLDSMIIIAAYHSVRDPFVLNIERGKKSGQQISRVLFAGDGMSDSLAVIKLYEEFTNLKRKDIGDFCERNFICRNAMEMFVSAASTLRDTVYRIFRFSEASARLASSFNNDTNMIRLALTAGLYPKLAYMDRENKNQLVAEGDPLVQVSRSSCLRGKKKQKDLASEWILFVEKTRTADQISSLEHTTLVSGLMVALAGGKRFITEAHGSEMLLCLDSWIRLKCPAEFGLQLHKVRMLTDREFAKLVATRKLSLMSDFIGPETVRRLLEADVPSTCLAEGKSLLDRDNCDNDF

Target ORF information:

RefSeq Version	NM_132980.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG8915), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132980.3

ORF Insert Sequence:

ATGGCGGACA GTGAAGAAGC AGTGAAACGT GGCCGTCGCC AGGGCAGAAA GAAGGCACAA 
CCTATGCCGG AACAGGCGGT AGTGTTGGTG GTCAATGGCC GAGTGACCGG TGGGCCGAAG 
GGAACTGGCA AACAGAAGAC TCGACCGGGC AAGAACGCGA AAGCCGACGC GGGCGACAAG 
CTGAAGTCCG AGGACGAAAA GATGCTCCGC CAACTGGTCG ACGACTTCCT GCAGAGCTGC 
GAGCCAGAGA AACAGCTCCC CGGACTTACA AAAGCCCAGC GCAGCCACGT TCACCGATTG 
GCCCAGCATC GCGGGCTTAA GACGGTGAGC AAGGGTCCAG AGGAGAACCG CGTCCTTTTT 
ATAAGTCGCC CACAGTCGGA TGGCCAACAG CAATACATTC TTTGCAACAC AAAGCTGGAC 
ATTTGTCCGC CGCTCCTAGA CGTTCTGGGA CAAATGGCCG GCAATGTGGA ACGCAGATTG 
ATGAACTCCG TTGGAGACTT AAAGTGCAAC AAACAGCAAT ATAAGGATAT ACGGCTTCCT 
TCCAACTTCG GCCTGGTCGG GCAGAGGCTT ATTCCGCCGC CGCAGAGCAA CCGGAACAGG 
AACATTCAGC ACGAGCGCCG GAGCCTGCCC ATCTACAAGC AGCGGGAGAG CATCCTCAAC 
GTTCTGCAGC GCGATCAGGT TTTGATCATT AAAGGAGCCA CCGGCTCTGG CAAGTCCACC 
CAACTTCCCC AGTACATCCT TGAGTGGGCC GCAGAGCATC GTGCTCCAGT CAGGATCGTG 
GTAAGTCAGC CTCGCCGCAT AGCAGCTATC AGTGTCTCGG AGCGCATTTC CAAGGAGCGC 
GGTGAGGCGC CTGGCACCAC AGTGGGCTAT AATATTCGCA TGAATAGACA ATGCAGCAGC 
AACACGGTTC TAATGCTGAC CACCAGTGGT TGCCTTTTAA GGGCTCTCGC CATGGATAAG 
AAGTCTTTTT TTAAAAACAC CACCCACTTA ATCATTGATG AAGCCCACGA GCGTGATTTG 
GACACAGACT TCCTGCTGCT GGCCACCAAG CTGGAGCTGC AAAAGAATCC GCACCTTCGC 
CTCGTGCTTA TGTCGGCCAC CATGGATCTT GAAGCATTGT CCAACTATTT TGGTGGGGGC 
ACTGTAATGG ATGTGGAGGG GCGCAGCTTC GAGGTGTCGA TCTATCATCT AGAGGACATT 
CTTAGTAAAA CTGGGTACAT GCACCCGAGA ATGGAGAAGT TTTTGGGCAA GCCGACAGGC 
AAAGAAACTC CTAGCGAACT ACTAGCCGCC TATTATGGAG GTAACACAAT CGTACATCCG 
GATATCGACA ATGACTTGAT CGTTTCGCTA TTGGAATTAT TACTGCGCCA AGGTGACGCT 
GGAGCGGTGA TTGTGTACCT GCCAGGCTAT AGCGATATGA CCTCGCTACT GGCCCGCTTG 
GAATCGTCTC TGCCGCGGGA ACAGATTACT ATCATACTGC TTCATAGCCA GGTGGACAAC 
AGCGAGCAAC GCAAGGTCTT CCGCACTTAT CCTGGCGTGC GTTTAAAGAT TATTCTTAGT 
ACCAACATAG GACAAACTTC GATCACCATT CCGGATCTAC TCTATGTCAT CGACACGGGC 
CTCGCCAAGA TGAAGACCTA TGACTCGACC ATAGATGCAT CGCAGTTAAC CCTCACGTGG 
ATCTCGCAGG CGGACGCCAA ACAGCGTGCG GGAAGAGCTG GACGCGTGTG CCATGGCAAT 
TGCTATCGTC TGTATGACAA CGACCGCATG GCCAGAATGA ATCTGTACAC CATCCCAGAG 
ATCATGAGGC GTACCTTGGA CGAGATATGT CTGCTGACCA AGTTGGCAGC TCCAGACAAG 
AAAATAGAAA ATTTTCTAGC TCTGGCTTTG GATACCCCAC CAAAGGATGC CGTAATGCAG 
TCCTGTTCTA GACTGAAGCT ATTGGGCGTG CTCGACGAAC GGGACGAGGT CACGCCGCTG 
GGACATATCG TTGCCGAACT GCCCTTAGGC GTTCAAATCG GCAAATGCCT GGTGTACTCC 
ATTTACTTGC GCTGCCTGGA CAGCATGATC ATAATTGCGG CCTACCACTC GGTGAGGGAT 
CCCTTTGTTC TAAACATAGA GCGCGGCAAG AAATCAGGGC AGCAGATATC CAGAGTTCTT 
TTCGCTGGCG ACGGAATGAG TGATTCGCTG GCGGTCATTA AACTCTACGA GGAGTTCACC 
AACTTAAAGC GCAAGGACAT CGGCGATTTT TGCGAGCGCA ATTTTATTTG CCGTAACGCA 
ATGGAGATGT TCGTCTCGGC GGCGAGTACT CTACGCGACA CCGTGTACCG TATCTTCCGC 
TTTAGCGAGG CCAGTGCTCG TTTGGCCTCG TCCTTCAACA ATGATACGAA TATGATCCGC 
CTGGCCCTGA CTGCAGGACT GTATCCCAAG TTGGCGTACA TGGATCGCGA GAACAAGAAC 
CAGCTGGTGG CTGAAGGAGA TCCATTAGTT CAGGTTTCGA GAAGCTCTTG CCTTCGGGGC 
AAAAAGAAGC AAAAGGATTT GGCCAGCGAG TGGATCCTGT TCGTTGAGAA GACCCGCACT 
GCTGACCAAA TTTCCTCGCT AGAGCATACC ACCTTGGTGA GCGGCTTGAT GGTGGCTCTG 
GCAGGCGGAA AACGGTTCAT AACCGAGGCG CATGGGTCCG AAATGCTGCT GTGCTTGGAC 
TCCTGGATCC GTTTGAAATG CCCAGCTGAA TTTGGACTCC AGCTCCATAA AGTGCGAATG 
CTAACGGATC GTGAATTCGC CAAGTTGGTA GCGACGCGGA AGCTTAGTCT AATGAGCGAT 
TTTATAGGGC CGGAAACAGT TCGGCGGTTG TTGGAGGCGG ATGTGCCCTC GACTTGCCTG 
GCCGAGGGCA AAAGCCTTCT GGACAGAGAC AACTGTGATA ACGACTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG8915 ( NM_001298440.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285369.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG8915), mRNA.

Price & Availability↑

CloneID	OFa01888
Clone ID Related Accession (Same CDS sequence)	NM_132980.3 , NM_001298440.1
Accession Version	NM_001298440.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2931bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGACA GTGAAGAAGC AGTGAAACGT GGCCGTCGCC AGGGCAGAAA GAAGGCACAA 
CCTATGCCGG AACAGGCGGT AGTGTTGGTG GTCAATGGCC GAGTGACCGG TGGGCCGAAG 
GGAACTGGCA AACAGAAGAC TCGACCGGGC AAGAACGCGA AAGCCGACGC GGGCGACAAG 
CTGAAGTCCG AGGACGAAAA GATGCTCCGC CAACTGGTCG ACGACTTCCT GCAGAGCTGC 
GAGCCAGAGA AACAGCTCCC CGGACTTACA AAAGCCCAGC GCAGCCACGT TCACCGATTG 
GCCCAGCATC GCGGGCTTAA GACGGTGAGC AAGGGTCCAG AGGAGAACCG CGTCCTTTTT 
ATAAGTCGCC CACAGTCGGA TGGCCAACAG CAATACATTC TTTGCAACAC AAAGCTGGAC 
ATTTGTCCGC CGCTCCTAGA CGTTCTGGGA CAAATGGCCG GCAATGTGGA ACGCAGATTG 
ATGAACTCCG TTGGAGACTT AAAGTGCAAC AAACAGCAAT ATAAGGATAT ACGGCTTCCT 
TCCAACTTCG GCCTGGTCGG GCAGAGGCTT ATTCCGCCGC CGCAGAGCAA CCGGAACAGG 
AACATTCAGC ACGAGCGCCG GAGCCTGCCC ATCTACAAGC AGCGGGAGAG CATCCTCAAC 
GTTCTGCAGC GCGATCAGGT TTTGATCATT AAAGGAGCCA CCGGCTCTGG CAAGTCCACC 
CAACTTCCCC AGTACATCCT TGAGTGGGCC GCAGAGCATC GTGCTCCAGT CAGGATCGTG 
GTAAGTCAGC CTCGCCGCAT AGCAGCTATC AGTGTCTCGG AGCGCATTTC CAAGGAGCGC 
GGTGAGGCGC CTGGCACCAC AGTGGGCTAT AATATTCGCA TGAATAGACA ATGCAGCAGC 
AACACGGTTC TAATGCTGAC CACCAGTGGT TGCCTTTTAA GGGCTCTCGC CATGGATAAG 
AAGTCTTTTT TTAAAAACAC CACCCACTTA ATCATTGATG AAGCCCACGA GCGTGATTTG 
GACACAGACT TCCTGCTGCT GGCCACCAAG CTGGAGCTGC AAAAGAATCC GCACCTTCGC 
CTCGTGCTTA TGTCGGCCAC CATGGATCTT GAAGCATTGT CCAACTATTT TGGTGGGGGC 
ACTGTAATGG ATGTGGAGGG GCGCAGCTTC GAGGTGTCGA TCTATCATCT AGAGGACATT 
CTTAGTAAAA CTGGGTACAT GCACCCGAGA ATGGAGAAGT TTTTGGGCAA GCCGACAGGC 
AAAGAAACTC CTAGCGAACT ACTAGCCGCC TATTATGGAG GTAACACAAT CGTACATCCG 
GATATCGACA ATGACTTGAT CGTTTCGCTA TTGGAATTAT TACTGCGCCA AGGTGACGCT 
GGAGCGGTGA TTGTGTACCT GCCAGGCTAT AGCGATATGA CCTCGCTACT GGCCCGCTTG 
GAATCGTCTC TGCCGCGGGA ACAGATTACT ATCATACTGC TTCATAGCCA GGTGGACAAC 
AGCGAGCAAC GCAAGGTCTT CCGCACTTAT CCTGGCGTGC GTTTAAAGAT TATTCTTAGT 
ACCAACATAG GACAAACTTC GATCACCATT CCGGATCTAC TCTATGTCAT CGACACGGGC 
CTCGCCAAGA TGAAGACCTA TGACTCGACC ATAGATGCAT CGCAGTTAAC CCTCACGTGG 
ATCTCGCAGG CGGACGCCAA ACAGCGTGCG GGAAGAGCTG GACGCGTGTG CCATGGCAAT 
TGCTATCGTC TGTATGACAA CGACCGCATG GCCAGAATGA ATCTGTACAC CATCCCAGAG 
ATCATGAGGC GTACCTTGGA CGAGATATGT CTGCTGACCA AGTTGGCAGC TCCAGACAAG 
AAAATAGAAA ATTTTCTAGC TCTGGCTTTG GATACCCCAC CAAAGGATGC CGTAATGCAG 
TCCTGTTCTA GACTGAAGCT ATTGGGCGTG CTCGACGAAC GGGACGAGGT CACGCCGCTG 
GGACATATCG TTGCCGAACT GCCCTTAGGC GTTCAAATCG GCAAATGCCT GGTGTACTCC 
ATTTACTTGC GCTGCCTGGA CAGCATGATC ATAATTGCGG CCTACCACTC GGTGAGGGAT 
CCCTTTGTTC TAAACATAGA GCGCGGCAAG AAATCAGGGC AGCAGATATC CAGAGTTCTT 
TTCGCTGGCG ACGGAATGAG TGATTCGCTG GCGGTCATTA AACTCTACGA GGAGTTCACC 
AACTTAAAGC GCAAGGACAT CGGCGATTTT TGCGAGCGCA ATTTTATTTG CCGTAACGCA 
ATGGAGATGT TCGTCTCGGC GGCGAGTACT CTACGCGACA CCGTGTACCG TATCTTCCGC 
TTTAGCGAGG CCAGTGCTCG TTTGGCCTCG TCCTTCAACA ATGATACGAA TATGATCCGC 
CTGGCCCTGA CTGCAGGACT GTATCCCAAG TTGGCGTACA TGGATCGCGA GAACAAGAAC 
CAGCTGGTGG CTGAAGGAGA TCCATTAGTT CAGGTTTCGA GAAGCTCTTG CCTTCGGGGC 
AAAAAGAAGC AAAAGGATTT GGCCAGCGAG TGGATCCTGT TCGTTGAGAA GACCCGCACT 
GCTGACCAAA TTTCCTCGCT AGAGCATACC ACCTTGGTGA GCGGCTTGAT GGTGGCTCTG 
GCAGGCGGAA AACGGTTCAT AACCGAGGCG CATGGGTCCG AAATGCTGCT GTGCTTGGAC 
TCCTGGATCC GTTTGAAATG CCCAGCTGAA TTTGGACTCC AGCTCCATAA AGTGCGAATG 
CTAACGGATC GTGAATTCGC CAAGTTGGTA GCGACGCGGA AGCTTAGTCT AATGAGCGAT 
TTTATAGGGC CGGAAACAGT TCGGCGGTTG TTGGAGGCGG ATGTGCCCTC GACTTGCCTG 
GCCGAGGGCA AAAGCCTTCT GGACAGAGAC AACTGTGATA ACGACTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285369.1
CDS	91..3021
Translation	MADSEEAVKRGRRQGRKKAQPMPEQAVVLVVNGRVTGGPKGTGKQKTRPGKNAKADAGDKLKSEDEKMLRQLVDDFLQSCEPEKQLPGLTKAQRSHVHRLAQHRGLKTVSKGPEENRVLFISRPQSDGQQQYILCNTKLDICPPLLDVLGQMAGNVERRLMNSVGDLKCNKQQYKDIRLPSNFGLVGQRLIPPPQSNRNRNIQHERRSLPIYKQRESILNVLQRDQVLIIKGATGSGKSTQLPQYILEWAAEHRAPVRIVVSQPRRIAAISVSERISKERGEAPGTTVGYNIRMNRQCSSNTVLMLTTSGCLLRALAMDKKSFFKNTTHLIIDEAHERDLDTDFLLLATKLELQKNPHLRLVLMSATMDLEALSNYFGGGTVMDVEGRSFEVSIYHLEDILSKTGYMHPRMEKFLGKPTGKETPSELLAAYYGGNTIVHPDIDNDLIVSLLELLLRQGDAGAVIVYLPGYSDMTSLLARLESSLPREQITIILLHSQVDNSEQRKVFRTYPGVRLKIILSTNIGQTSITIPDLLYVIDTGLAKMKTYDSTIDASQLTLTWISQADAKQRAGRAGRVCHGNCYRLYDNDRMARMNLYTIPEIMRRTLDEICLLTKLAAPDKKIENFLALALDTPPKDAVMQSCSRLKLLGVLDERDEVTPLGHIVAELPLGVQIGKCLVYSIYLRCLDSMIIIAAYHSVRDPFVLNIERGKKSGQQISRVLFAGDGMSDSLAVIKLYEEFTNLKRKDIGDFCERNFICRNAMEMFVSAASTLRDTVYRIFRFSEASARLASSFNNDTNMIRLALTAGLYPKLAYMDRENKNQLVAEGDPLVQVSRSSCLRGKKKQKDLASEWILFVEKTRTADQISSLEHTTLVSGLMVALAGGKRFITEAHGSEMLLCLDSWIRLKCPAEFGLQLHKVRMLTDREFAKLVATRKLSLMSDFIGPETVRRLLEADVPSTCLAEGKSLLDRDNCDNDF

Target ORF information:

RefSeq Version	NM_001298440.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG8915), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298440.1

ORF Insert Sequence:

ATGGCGGACA GTGAAGAAGC AGTGAAACGT GGCCGTCGCC AGGGCAGAAA GAAGGCACAA 
CCTATGCCGG AACAGGCGGT AGTGTTGGTG GTCAATGGCC GAGTGACCGG TGGGCCGAAG 
GGAACTGGCA AACAGAAGAC TCGACCGGGC AAGAACGCGA AAGCCGACGC GGGCGACAAG 
CTGAAGTCCG AGGACGAAAA GATGCTCCGC CAACTGGTCG ACGACTTCCT GCAGAGCTGC 
GAGCCAGAGA AACAGCTCCC CGGACTTACA AAAGCCCAGC GCAGCCACGT TCACCGATTG 
GCCCAGCATC GCGGGCTTAA GACGGTGAGC AAGGGTCCAG AGGAGAACCG CGTCCTTTTT 
ATAAGTCGCC CACAGTCGGA TGGCCAACAG CAATACATTC TTTGCAACAC AAAGCTGGAC 
ATTTGTCCGC CGCTCCTAGA CGTTCTGGGA CAAATGGCCG GCAATGTGGA ACGCAGATTG 
ATGAACTCCG TTGGAGACTT AAAGTGCAAC AAACAGCAAT ATAAGGATAT ACGGCTTCCT 
TCCAACTTCG GCCTGGTCGG GCAGAGGCTT ATTCCGCCGC CGCAGAGCAA CCGGAACAGG 
AACATTCAGC ACGAGCGCCG GAGCCTGCCC ATCTACAAGC AGCGGGAGAG CATCCTCAAC 
GTTCTGCAGC GCGATCAGGT TTTGATCATT AAAGGAGCCA CCGGCTCTGG CAAGTCCACC 
CAACTTCCCC AGTACATCCT TGAGTGGGCC GCAGAGCATC GTGCTCCAGT CAGGATCGTG 
GTAAGTCAGC CTCGCCGCAT AGCAGCTATC AGTGTCTCGG AGCGCATTTC CAAGGAGCGC 
GGTGAGGCGC CTGGCACCAC AGTGGGCTAT AATATTCGCA TGAATAGACA ATGCAGCAGC 
AACACGGTTC TAATGCTGAC CACCAGTGGT TGCCTTTTAA GGGCTCTCGC CATGGATAAG 
AAGTCTTTTT TTAAAAACAC CACCCACTTA ATCATTGATG AAGCCCACGA GCGTGATTTG 
GACACAGACT TCCTGCTGCT GGCCACCAAG CTGGAGCTGC AAAAGAATCC GCACCTTCGC 
CTCGTGCTTA TGTCGGCCAC CATGGATCTT GAAGCATTGT CCAACTATTT TGGTGGGGGC 
ACTGTAATGG ATGTGGAGGG GCGCAGCTTC GAGGTGTCGA TCTATCATCT AGAGGACATT 
CTTAGTAAAA CTGGGTACAT GCACCCGAGA ATGGAGAAGT TTTTGGGCAA GCCGACAGGC 
AAAGAAACTC CTAGCGAACT ACTAGCCGCC TATTATGGAG GTAACACAAT CGTACATCCG 
GATATCGACA ATGACTTGAT CGTTTCGCTA TTGGAATTAT TACTGCGCCA AGGTGACGCT 
GGAGCGGTGA TTGTGTACCT GCCAGGCTAT AGCGATATGA CCTCGCTACT GGCCCGCTTG 
GAATCGTCTC TGCCGCGGGA ACAGATTACT ATCATACTGC TTCATAGCCA GGTGGACAAC 
AGCGAGCAAC GCAAGGTCTT CCGCACTTAT CCTGGCGTGC GTTTAAAGAT TATTCTTAGT 
ACCAACATAG GACAAACTTC GATCACCATT CCGGATCTAC TCTATGTCAT CGACACGGGC 
CTCGCCAAGA TGAAGACCTA TGACTCGACC ATAGATGCAT CGCAGTTAAC CCTCACGTGG 
ATCTCGCAGG CGGACGCCAA ACAGCGTGCG GGAAGAGCTG GACGCGTGTG CCATGGCAAT 
TGCTATCGTC TGTATGACAA CGACCGCATG GCCAGAATGA ATCTGTACAC CATCCCAGAG 
ATCATGAGGC GTACCTTGGA CGAGATATGT CTGCTGACCA AGTTGGCAGC TCCAGACAAG 
AAAATAGAAA ATTTTCTAGC TCTGGCTTTG GATACCCCAC CAAAGGATGC CGTAATGCAG 
TCCTGTTCTA GACTGAAGCT ATTGGGCGTG CTCGACGAAC GGGACGAGGT CACGCCGCTG 
GGACATATCG TTGCCGAACT GCCCTTAGGC GTTCAAATCG GCAAATGCCT GGTGTACTCC 
ATTTACTTGC GCTGCCTGGA CAGCATGATC ATAATTGCGG CCTACCACTC GGTGAGGGAT 
CCCTTTGTTC TAAACATAGA GCGCGGCAAG AAATCAGGGC AGCAGATATC CAGAGTTCTT 
TTCGCTGGCG ACGGAATGAG TGATTCGCTG GCGGTCATTA AACTCTACGA GGAGTTCACC 
AACTTAAAGC GCAAGGACAT CGGCGATTTT TGCGAGCGCA ATTTTATTTG CCGTAACGCA 
ATGGAGATGT TCGTCTCGGC GGCGAGTACT CTACGCGACA CCGTGTACCG TATCTTCCGC 
TTTAGCGAGG CCAGTGCTCG TTTGGCCTCG TCCTTCAACA ATGATACGAA TATGATCCGC 
CTGGCCCTGA CTGCAGGACT GTATCCCAAG TTGGCGTACA TGGATCGCGA GAACAAGAAC 
CAGCTGGTGG CTGAAGGAGA TCCATTAGTT CAGGTTTCGA GAAGCTCTTG CCTTCGGGGC 
AAAAAGAAGC AAAAGGATTT GGCCAGCGAG TGGATCCTGT TCGTTGAGAA GACCCGCACT 
GCTGACCAAA TTTCCTCGCT AGAGCATACC ACCTTGGTGA GCGGCTTGAT GGTGGCTCTG 
GCAGGCGGAA AACGGTTCAT AACCGAGGCG CATGGGTCCG AAATGCTGCT GTGCTTGGAC 
TCCTGGATCC GTTTGAAATG CCCAGCTGAA TTTGGACTCC AGCTCCATAA AGTGCGAATG 
CTAACGGATC GTGAATTCGC CAAGTTGGTA GCGACGCGGA AGCTTAGTCT AATGAGCGAT 
TTTATAGGGC CGGAAACAGT TCGGCGGTTG TTGGAGGCGG ATGTGCCCTC GACTTGCCTG 
GCCGAGGGCA AAAGCCTTCT GGACAGAGAC AACTGTGATA ACGACTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG8915 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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