baz cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	baz
Entrez Gene ID	32703
Full Name	bazooka
Synonyms	BAZ,Baz,Baz/Par-3,Baz/Par3,Bazooka,CG5055,D-Par3,D-par3,Dmel\CG5055,PAR-3,PAR3,Par-3,Par3,dPar-3,l(1)G0484,par-3,par3
General protein information	Preferred Names bazooka Names CG5055 gene product from transcript CG5055-RA Bazooka-Par3 Bazooka/Par-3 Bazooka/Par3 CG5055-PA CG5055-PB CG5055-PC CG5055-PD baz-PA baz-PB baz-PC baz-PD par-3
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 15F1-15F1\|1-56.7 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001298435.2	NP_001285364.1	bazooka, isoform C
NM_001042818.4	NP_001036283.1	bazooka, isoform B
NM_001298436.2	NP_001285365.1	bazooka, isoform D
NM_001347807.1	NP_001334669.1	bazooka, isoform A

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	baz	NP_523383.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000869	XP_316842.5

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Baz protein in the mesoderm is down-regulated from junctional sites in response to Snail expression. This down-regulation leads to a specific decrease in adherens junctions without affecting other E-Cadherin pools.
Title: Polarity protein Par3/Bazooka follows myosin-dependent junction repositioning.

Par3/Baz CR3 can antagonize aPKC in stable apical Par complexes and modulation of CR3 inhibitory arms or opposing aPKC pockets would perturb the interaction, promoting Par3/Baz phosphorylation.
Title: aPKC Inhibition by Par3 CR3 Flanking Regions Controls Substrate Access and Underpins Apical-Junctional Polarization.

overexpression of Magi resulted in the displacement of Baz/Par3 and aPKC and lead to an increase in the level of PIP3. we found that Magi and Baz functioned in an antagonistic manner to regulate the localization of the apical polarity complex.
Title: Magi Is Associated with the Par Complex and Functions Antagonistically with Bazooka to Regulate the Apical Polarity Complex.

aPKC prevents hyper-polarization of junctions as epithelia develop from cellularization to gastrulation by inhibiting a positive feedback loop between Bazooka (Baz)/Par-3 and centrosomes. This loop is driven by Par-1, a kinase known to phosphorylate Baz.
Title: A Par-1-Par-3-Centrosome Cell Polarity Pathway and Its Tuning for Isotropic Cell Adhesion.

Bazooka (Baz) provides a platform for the correct centrosome orientation and that Baz-centrosome association is the key event that is monitored by the centrosome orientation checkpoint.
Title: The polarity protein Baz forms a platform for the centrosome orientation during asymmetric stem cell division in the Drosophila male germline.

The following baz gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the baz cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01876	NM_001347807.1 Latest version!	Drosophila melanogaster bazooka (baz), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa01877	NM_001042818.4 Latest version!	Drosophila melanogaster bazooka (baz), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa21513	NM_001298436.2 Latest version!	Drosophila melanogaster bazooka (baz), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa21514	NM_001298435.2 Latest version!	Drosophila melanogaster bazooka (baz), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

baz ( NM_001347807.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001334669.1 Drosophila melanogaster bazooka (baz), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa01876
Clone ID Related Accession (Same CDS sequence)	NM_001347807.1
Accession Version	NM_001347807.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	bazooka, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGGTCA CCGTCTGCTT CGGCGACGTT CGCATTCTGG TTCCCTGTGG TTCCGGCGAA 
CTCCTCGTCC GCGATCTCGT CAAGGAGGCC ACGCGACGCT ACATCAAGGC GGCCGGCAAG 
CCCGATTCCT GGGTGACCGT GACGCATCTC CAGACACAGT CGGGCATCCT CGATCCGGAT 
GATTGTGTCC GCGACGTGGC CGACGATCGG GAGCAGATAT TGGCGCACTT TGATGACCCA 
GGACCCGATC CAGGAGTTCC GCAAGGAGGC GGAGATGGAG CATCGGGCAG TTCGTCCGTT 
GGCACCGGTT CGCCGGACAT CTTTCGCGAT CCCACCAACA CGGAGGCGCC CACCTGTCCG 
CGGGATCTCT CCACGCCACA CATCGAGGTC ACCAGCACCA CATCGGGACC GATGGCTGGA 
CTCGGAGTTG GACTGATGGT GCGTCGCAGC AGTGATCCCA ATCTGCTGGC CTCGCTGAAG 
GCAGAGGGCA GTAACAAACG CTGGTCGGCG GCGGCTCCCC ACTACGCTGG CGGGGATTCG 
CCGGAGCGCC TGTTTCTTGA CAAGGCCGGT GGCCAGTTAT CGCCACAGTG GGAGGAGGAC 
GACGATCCCA GTCATCAGCT AAAGGAACAG CTGCTACATC AACAGCAGCC CCATGCTGCT 
AATGGAGGCT CCTCCTCCGG TAACCATCAG CCGTTTGCCC GATCCGGTCG CCTGTCGATG 
CAATTTCTTG GCGATGGCAA TGGCTACAAG TGGATGGAGG CAGCCGAGAA ACTTCAGAAT 
CAGCCGCCAG CCCAGCAGAC ATATCAGCAG GGTTCTCATC ATGCTGGTCA TGGTCAAAAC 
GGTGCCTACT CCAGCAAGTC CTTGCCCAGG GAGAGCAAGC GAAAGGAGCC CTTGGGACAG 
GCATATGAAT CCATCAGGGA GAAGGATGGT GAAATGCTGC TGATCATCAA CGAGTACGGC 
AGTCCGCTGG GACTAACTGC TCTGCCGGAC AAGGAGCATG GCGGTGGACT GTTGGTGCAA 
CATGTGGAGC CGGGCAGCCG AGCCGAGAGA GGACGACTGC GTCGTGATGA TCGCATCTTG 
GAGATCAATG GAATCAAGCT AATAGGACTC ACCGAATCGC AGGTTCAGGA GCAACTGCGG 
CGGGCTTTGG AGAGCTCAGA GTTGAGAGTT CGAGTGCTGC GCGGTGATCG CAATCGCCGC 
CAACAGCGTG ACTCTAAGGT GGCCGAGATG GTGGAGGTGG CTACGGTGTC ACCCACCCGC 
AAACCACATG CTGCTCCGGT GGGCACCTCG CTGCAGGTGG CCAATACCCG TAAACTGGGC 
AGGAAAATCG AAATTATGCT CAAGAAGGGA CCCAACGGTC TGGGCTTTTC GGTCACAACA 
CGCGATAATC CCGCCGGTGG CCACTGTCCC ATCTACATCA AGAATATCCT GCCACGAGGT 
GCGGCCATCG AGGATGGACG CCTGAAGCCC GGCGATCGTT TGCTCGAGGT GGATGGCACT 
CCAATGACTG GTAAAACACA AACGGATGTG GTGGCAATCT TGAGGGGCAT GCCAGCGGGA 
GCAACCGTAA GGATTGTGGT CTCCCGCCAG CAGGAGTTGG CGGAGCAGGC TGACCAGCCG 
GCGGAGAAAA GTGCTGGTGT GGCGGTGGCG CCCTCAGTTG CTCCACCAGC TGTTCCTGCA 
GCTGCAGCTC CAGCGCCTCC CATTCCGGTA CAAAAATCCA GCAGCGCACG TTCTCTGTTC 
ACTCATCAGC AGCAATCGCA GCTCAACGAA TCTCAGCACT TTATTGATGC GGGCAGCGAG 
TCGGCGGCTT CAAATGACAG CCTGCCGCCC AGCAGCAACA GTTGGCACTC CCGCGAGGAG 
CTGACCCTGC ACATTCCCGT ACACGACACC GAAAAGGCCG GACTGGGTGT CAGTGTGAAG 
GGAAAGACGT GCTCGAATCT GAACGCTTCC GGATCGAGTG CCTCCAGCGG CAGCAATGGA 
CTGATGAAGC ATGACGGTGA CTTGGGTATA TTCGTGAAGA ATGTAATTCA TGGCGGTGCT 
GCATCCCGCG ATGGTCGCTT GCGGATGAAT GATCAACTGC TGAGCGTGAA TGGAGTATCG 
CTGCGTGGGC AAAACAATGC CGAGGCCATG GAGACACTAC GTCGGGCAAT GGTCAACACG 
CCCGGCAAAC ATCCGGGAAC CATAACCCTG CTGGTAGGCC GCAAGATCTT GCGATCGGCC 
AGTTCCAGTG ACATTCTGGA CCACAGTAAC AGTCACAGTC ATAGCCATAG CAATAGTAGC 
GGTGGCAGCA ATTCAAATGG TAGCGGCAAT AACAACAATA GCAGCTCGAA TGCCAGCGAT 
AATTCTGGAG CCACGGTCAT ATATTTGAGT CCGGAGAAGA GGGAGCAGCG CTGCAATGGC 
GGCGGAGGTG GTGGCAGTGC TGGCAATGAG ATGAATAGAT GGAGCAATCC CGTTTTGGAT 
CGCCTAACCG GTGGCATTTG CTCCTCGAAT TCAGCGCAGC CATCCTCTCA GCAGTCTCAC 
CAGCAGCAGC CGCATCCTTC GCAGCAGCAA CAGCAGCAAC GTCGCCTGCC TGCAGCGCCC 
GTTTGCAGCA GTGCAGCTCT GAGAAACGAG AGCTACTACA TGGCCACCAA TGACAACTGG 
TCGCCGGCGC AGATGCACTT AATGACTGCT CATGGCAACA CGGCGCTGCT TATCGAGGAC 
GATGCCGAGC CGATGTCTCC AACACTACCG GCACGTCCGC ATGATGGGCA GCACTGCAAC 
ACGAGCAGTG CTAATCCATC GCAGAACTTG GCCGTCGGCA ATCAGGGGCC ACCCATAAAT 
ACCGTGCCTG GTACTCCGTC GACATCCAGC AACTTTGATG CCACCTACTC CTCGCAACTG 
AGCTTGGAGA CAAACTCGGG CGTGGAGCAT TTCTCGCGCG ATGCTTTGGG ACGACGCAGC 
ATCTCTGAGA AGCACCATGC GGCGCTGGAT GCCCGCGAAA CTGGCACCTA TCAGCGGAAT 
AAGAAGTTAC GCGAGGAGCG CGAACGCGAG CGTCGCATTC AGCTGACCAA ATCGGCTGTG 
TATGGTGGTT CCATTGAGTC CCTTACGGCT CGCATAGCCA GTGCTAATGC CCAGTTTTCG 
GGATATAAAC ATGCCAAGAC TGCATCCAGT ATCGAGCAAA GAGAGACGCA GCAGCAATTG 
GCCGCTGCGG AGGCGGAGGC AAGGGATCAG CTGGGCGATC TGGGTCCATC GCTGGGCATG 
AAGAAGTCCT CGTCGTTGGA GTCGCTCCAG ACTATGGTGC AGGAGCTGCA GATGTCGGAT 
GAGCCGCGTG GTCATCAGGC GTTGCGCGCA CCGCGTGGGC GTGGCAGGGA GGACAGTCTG 
CGGGCGGCGG TGGTCAGCGA ACCGGATGCG AGCAAGCCCC GCAAGACCTG GCTTTTGGAG 
GATGGCGATC ACGAGGGTGG TTTTGCCTCG CAGCGCAATG GACCGTTCCA GAGTTCCCTG 
AACGATGGCA AACACGGCTG CAAGTCGTCG CGGGCCAAGA AGCCAAGCAT ACTGCGCGGT 
ATCGGTCACA TGTTCCGCTT TGGCAAGAAT CGCAAGGATG GCGTGGTGCC AGTGGATAAC 
TATGCGGTAA ACATTTCGCC CCCTACATCG GTGGTTTCCA CAGCCACATC GCCGCAGCTG 
CAGCAGCAGC AACAGCAGCA GTTGCAGCAA CATCAGCAGC AACAACAGAT ACCAACCGCT 
GCATTGGCTG CTCTGGAGAG AAATGGCAAA CCGCCGGCGT ATCAGCCACC GCCACCGCTG 
CCTGCTCCAA ATGGAGTGGG TAGCAATGGT ATCCATCAGA ACGACATCTT CAACCATCGC 
TATCAGCATT ATGCCAACTA CGAGGACCTC CACCAGCAGC ACCAGCAACA CCAGATTAGT 
CGCCGCCATC AGCATTACCA TTCGCAGCGC AGTGCCCGCT CGCAGGATGT GAGCATGCAC 
TCGACGAGCT CCGGATCCCA GCCAGGATCC CTGGCTCAGC CGCAAGCGCA ATCAAACGGC 
GTTCGTCCCA TGAGCAGTTA CTACGAGTAC GAGACGGTTC AACAGCAACG GGTGGGCAGC 
ATCAAGCATA GCCACAGCAG CAGCGCCACG TCGTCCTCCT CGTCGCCCAT CAATGTGCCG 
CATTGGAAGG CGGCAGCCAT GAATGGCTAC TCGCCAGCCA GCCTGAATAG CAGTGCCCGG 
AGTCGAGGTC CGTTTGTGAC GCAGGTGACC ATACGGGAGC AGAGCAGTGG CGGCATACCC 
GCCCACCTGC TGCAGCAACA TCAGCAGCAG CAACTCCAGC AGCAGCAGCC CACCTACCAG 
ACTGTTCAGA AGATGTCAGG ACCATCGCAA TATGGTAGTG CCGCCGGTTC CCAGCCACAC 
GCCTCCAAGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001334669.1
CDS	1067..5461
Translation	MKVTVCFGDVRILVPCGSGELLVRDLVKEATRRYIKAAGKPDSWVTVTHLQTQSGILDPDDCVRDVADDREQILAHFDDPGPDPGVPQGGGDGASGSSSVGTGSPDIFRDPTNTEAPTCPRDLSTPHIEVTSTTSGPMAGLGVGLMVRRSSDPNLLASLKAEGSNKRWSAAAPHYAGGDSPERLFLDKAGGQLSPQWEEDDDPSHQLKEQLLHQQQPHAANGGSSSGNHQPFARSGRLSMQFLGDGNGYKWMEAAEKLQNQPPAQQTYQQGSHHAGHGQNGAYSSKSLPRESKRKEPLGQAYESIREKDGEMLLIINEYGSPLGLTALPDKEHGGGLLVQHVEPGSRAERGRLRRDDRILEINGIKLIGLTESQVQEQLRRALESSELRVRVLRGDRNRRQQRDSKVAEMVEVATVSPTRKPHAAPVGTSLQVANTRKLGRKIEIMLKKGPNGLGFSVTTRDNPAGGHCPIYIKNILPRGAAIEDGRLKPGDRLLEVDGTPMTGKTQTDVVAILRGMPAGATVRIVVSRQQELAEQADQPAEKSAGVAVAPSVAPPAVPAAAAPAPPIPVQKSSSARSLFTHQQQSQLNESQHFIDAGSESAASNDSLPPSSNSWHSREELTLHIPVHDTEKAGLGVSVKGKTCSNLNASGSSASSGSNGLMKHDGDLGIFVKNVIHGGAASRDGRLRMNDQLLSVNGVSLRGQNNAEAMETLRRAMVNTPGKHPGTITLLVGRKILRSASSSDILDHSNSHSHSHSNSSGGSNSNGSGNNNNSSSNASDNSGATVIYLSPEKREQRCNGGGGGGSAGNEMNRWSNPVLDRLTGGICSSNSAQPSSQQSHQQQPHPSQQQQQQRRLPAAPVCSSAALRNESYYMATNDNWSPAQMHLMTAHGNTALLIEDDAEPMSPTLPARPHDGQHCNTSSANPSQNLAVGNQGPPINTVPGTPSTSSNFDATYSSQLSLETNSGVEHFSRDALGRRSISEKHHAALDARETGTYQRNKKLREERERERRIQLTKSAVYGGSIESLTARIASANAQFSGYKHAKTASSIEQRETQQQLAAAEAEARDQLGDLGPSLGMKKSSSLESLQTMVQELQMSDEPRGHQALRAPRGRGREDSLRAAVVSEPDASKPRKTWLLEDGDHEGGFASQRNGPFQSSLNDGKHGCKSSRAKKPSILRGIGHMFRFGKNRKDGVVPVDNYAVNISPPTSVVSTATSPQLQQQQQQQLQQHQQQQQIPTAALAALERNGKPPAYQPPPPLPAPNGVGSNGIHQNDIFNHRYQHYANYEDLHQQHQQHQISRRHQHYHSQRSARSQDVSMHSTSSGSQPGSLAQPQAQSNGVRPMSSYYEYETVQQQRVGSIKHSHSSSATSSSSSPINVPHWKAAAMNGYSPASLNSSARSRGPFVTQVTIREQSSGGIPAHLLQQHQQQQLQQQQPTYQTVQKMSGPSQYGSAAGSQPHASKV

Target ORF information:

RefSeq Version	NM_001347807.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster bazooka (baz), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001347807.1

ORF Insert Sequence:

ATGAAGGTCA CCGTCTGCTT CGGCGACGTT CGCATTCTGG TTCCCTGTGG TTCCGGCGAA 
CTCCTCGTCC GCGATCTCGT CAAGGAGGCC ACGCGACGCT ACATCAAGGC GGCCGGCAAG 
CCCGATTCCT GGGTGACCGT GACGCATCTC CAGACACAGT CGGGCATCCT CGATCCGGAT 
GATTGTGTCC GCGACGTGGC CGACGATCGG GAGCAGATAT TGGCGCACTT TGATGACCCA 
GGACCCGATC CAGGAGTTCC GCAAGGAGGC GGAGATGGAG CATCGGGCAG TTCGTCCGTT 
GGCACCGGTT CGCCGGACAT CTTTCGCGAT CCCACCAACA CGGAGGCGCC CACCTGTCCG 
CGGGATCTCT CCACGCCACA CATCGAGGTC ACCAGCACCA CATCGGGACC GATGGCTGGA 
CTCGGAGTTG GACTGATGGT GCGTCGCAGC AGTGATCCCA ATCTGCTGGC CTCGCTGAAG 
GCAGAGGGCA GTAACAAACG CTGGTCGGCG GCGGCTCCCC ACTACGCTGG CGGGGATTCG 
CCGGAGCGCC TGTTTCTTGA CAAGGCCGGT GGCCAGTTAT CGCCACAGTG GGAGGAGGAC 
GACGATCCCA GTCATCAGCT AAAGGAACAG CTGCTACATC AACAGCAGCC CCATGCTGCT 
AATGGAGGCT CCTCCTCCGG TAACCATCAG CCGTTTGCCC GATCCGGTCG CCTGTCGATG 
CAATTTCTTG GCGATGGCAA TGGCTACAAG TGGATGGAGG CAGCCGAGAA ACTTCAGAAT 
CAGCCGCCAG CCCAGCAGAC ATATCAGCAG GGTTCTCATC ATGCTGGTCA TGGTCAAAAC 
GGTGCCTACT CCAGCAAGTC CTTGCCCAGG GAGAGCAAGC GAAAGGAGCC CTTGGGACAG 
GCATATGAAT CCATCAGGGA GAAGGATGGT GAAATGCTGC TGATCATCAA CGAGTACGGC 
AGTCCGCTGG GACTAACTGC TCTGCCGGAC AAGGAGCATG GCGGTGGACT GTTGGTGCAA 
CATGTGGAGC CGGGCAGCCG AGCCGAGAGA GGACGACTGC GTCGTGATGA TCGCATCTTG 
GAGATCAATG GAATCAAGCT AATAGGACTC ACCGAATCGC AGGTTCAGGA GCAACTGCGG 
CGGGCTTTGG AGAGCTCAGA GTTGAGAGTT CGAGTGCTGC GCGGTGATCG CAATCGCCGC 
CAACAGCGTG ACTCTAAGGT GGCCGAGATG GTGGAGGTGG CTACGGTGTC ACCCACCCGC 
AAACCACATG CTGCTCCGGT GGGCACCTCG CTGCAGGTGG CCAATACCCG TAAACTGGGC 
AGGAAAATCG AAATTATGCT CAAGAAGGGA CCCAACGGTC TGGGCTTTTC GGTCACAACA 
CGCGATAATC CCGCCGGTGG CCACTGTCCC ATCTACATCA AGAATATCCT GCCACGAGGT 
GCGGCCATCG AGGATGGACG CCTGAAGCCC GGCGATCGTT TGCTCGAGGT GGATGGCACT 
CCAATGACTG GTAAAACACA AACGGATGTG GTGGCAATCT TGAGGGGCAT GCCAGCGGGA 
GCAACCGTAA GGATTGTGGT CTCCCGCCAG CAGGAGTTGG CGGAGCAGGC TGACCAGCCG 
GCGGAGAAAA GTGCTGGTGT GGCGGTGGCG CCCTCAGTTG CTCCACCAGC TGTTCCTGCA 
GCTGCAGCTC CAGCGCCTCC CATTCCGGTA CAAAAATCCA GCAGCGCACG TTCTCTGTTC 
ACTCATCAGC AGCAATCGCA GCTCAACGAA TCTCAGCACT TTATTGATGC GGGCAGCGAG 
TCGGCGGCTT CAAATGACAG CCTGCCGCCC AGCAGCAACA GTTGGCACTC CCGCGAGGAG 
CTGACCCTGC ACATTCCCGT ACACGACACC GAAAAGGCCG GACTGGGTGT CAGTGTGAAG 
GGAAAGACGT GCTCGAATCT GAACGCTTCC GGATCGAGTG CCTCCAGCGG CAGCAATGGA 
CTGATGAAGC ATGACGGTGA CTTGGGTATA TTCGTGAAGA ATGTAATTCA TGGCGGTGCT 
GCATCCCGCG ATGGTCGCTT GCGGATGAAT GATCAACTGC TGAGCGTGAA TGGAGTATCG 
CTGCGTGGGC AAAACAATGC CGAGGCCATG GAGACACTAC GTCGGGCAAT GGTCAACACG 
CCCGGCAAAC ATCCGGGAAC CATAACCCTG CTGGTAGGCC GCAAGATCTT GCGATCGGCC 
AGTTCCAGTG ACATTCTGGA CCACAGTAAC AGTCACAGTC ATAGCCATAG CAATAGTAGC 
GGTGGCAGCA ATTCAAATGG TAGCGGCAAT AACAACAATA GCAGCTCGAA TGCCAGCGAT 
AATTCTGGAG CCACGGTCAT ATATTTGAGT CCGGAGAAGA GGGAGCAGCG CTGCAATGGC 
GGCGGAGGTG GTGGCAGTGC TGGCAATGAG ATGAATAGAT GGAGCAATCC CGTTTTGGAT 
CGCCTAACCG GTGGCATTTG CTCCTCGAAT TCAGCGCAGC CATCCTCTCA GCAGTCTCAC 
CAGCAGCAGC CGCATCCTTC GCAGCAGCAA CAGCAGCAAC GTCGCCTGCC TGCAGCGCCC 
GTTTGCAGCA GTGCAGCTCT GAGAAACGAG AGCTACTACA TGGCCACCAA TGACAACTGG 
TCGCCGGCGC AGATGCACTT AATGACTGCT CATGGCAACA CGGCGCTGCT TATCGAGGAC 
GATGCCGAGC CGATGTCTCC AACACTACCG GCACGTCCGC ATGATGGGCA GCACTGCAAC 
ACGAGCAGTG CTAATCCATC GCAGAACTTG GCCGTCGGCA ATCAGGGGCC ACCCATAAAT 
ACCGTGCCTG GTACTCCGTC GACATCCAGC AACTTTGATG CCACCTACTC CTCGCAACTG 
AGCTTGGAGA CAAACTCGGG CGTGGAGCAT TTCTCGCGCG ATGCTTTGGG ACGACGCAGC 
ATCTCTGAGA AGCACCATGC GGCGCTGGAT GCCCGCGAAA CTGGCACCTA TCAGCGGAAT 
AAGAAGTTAC GCGAGGAGCG CGAACGCGAG CGTCGCATTC AGCTGACCAA ATCGGCTGTG 
TATGGTGGTT CCATTGAGTC CCTTACGGCT CGCATAGCCA GTGCTAATGC CCAGTTTTCG 
GGATATAAAC ATGCCAAGAC TGCATCCAGT ATCGAGCAAA GAGAGACGCA GCAGCAATTG 
GCCGCTGCGG AGGCGGAGGC AAGGGATCAG CTGGGCGATC TGGGTCCATC GCTGGGCATG 
AAGAAGTCCT CGTCGTTGGA GTCGCTCCAG ACTATGGTGC AGGAGCTGCA GATGTCGGAT 
GAGCCGCGTG GTCATCAGGC GTTGCGCGCA CCGCGTGGGC GTGGCAGGGA GGACAGTCTG 
CGGGCGGCGG TGGTCAGCGA ACCGGATGCG AGCAAGCCCC GCAAGACCTG GCTTTTGGAG 
GATGGCGATC ACGAGGGTGG TTTTGCCTCG CAGCGCAATG GACCGTTCCA GAGTTCCCTG 
AACGATGGCA AACACGGCTG CAAGTCGTCG CGGGCCAAGA AGCCAAGCAT ACTGCGCGGT 
ATCGGTCACA TGTTCCGCTT TGGCAAGAAT CGCAAGGATG GCGTGGTGCC AGTGGATAAC 
TATGCGGTAA ACATTTCGCC CCCTACATCG GTGGTTTCCA CAGCCACATC GCCGCAGCTG 
CAGCAGCAGC AACAGCAGCA GTTGCAGCAA CATCAGCAGC AACAACAGAT ACCAACCGCT 
GCATTGGCTG CTCTGGAGAG AAATGGCAAA CCGCCGGCGT ATCAGCCACC GCCACCGCTG 
CCTGCTCCAA ATGGAGTGGG TAGCAATGGT ATCCATCAGA ACGACATCTT CAACCATCGC 
TATCAGCATT ATGCCAACTA CGAGGACCTC CACCAGCAGC ACCAGCAACA CCAGATTAGT 
CGCCGCCATC AGCATTACCA TTCGCAGCGC AGTGCCCGCT CGCAGGATGT GAGCATGCAC 
TCGACGAGCT CCGGATCCCA GCCAGGATCC CTGGCTCAGC CGCAAGCGCA ATCAAACGGC 
GTTCGTCCCA TGAGCAGTTA CTACGAGTAC GAGACGGTTC AACAGCAACG GGTGGGCAGC 
ATCAAGCATA GCCACAGCAG CAGCGCCACG TCGTCCTCCT CGTCGCCCAT CAATGTGCCG 
CATTGGAAGG CGGCAGCCAT GAATGGCTAC TCGCCAGCCA GCCTGAATAG CAGTGCCCGG 
AGTCGAGGTC CGTTTGTGAC GCAGGTGACC ATACGGGAGC AGAGCAGTGG CGGCATACCC 
GCCCACCTGC TGCAGCAACA TCAGCAGCAG CAACTCCAGC AGCAGCAGCC CACCTACCAG 
ACTGTTCAGA AGATGTCAGG ACCATCGCAA TATGGTAGTG CCGCCGGTTC CCAGCCACAC 
GCCTCCAAGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

baz ( NM_001042818.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001036283.1 Drosophila melanogaster bazooka (baz), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa01877
Clone ID Related Accession (Same CDS sequence)	NM_001042818.4
Accession Version	NM_001042818.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	bazooka, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Dec 13, 2016 this sequence version replaced NM_001042818.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCGACG TACCGCCAAA GTGTCCTGCG TTGGCCAACA AGCTGGGCGG TCTCTTTGGG 
TGGCGACACA CCTACAAGGT GGCCGCCAAG CAGGAGGGGA TTCCCCATGG GCAGCTACAC 
AAGTCCTACT CCCTCACGCT GCCCAAGCGA CCGCCTTCGC CATCGACCTA TTCGTGTGTG 
AAGCCCGATT CCTGGGTGAC CGTGACGCAT CTCCAGACAC AGTCGGGCAT CCTCGATCCG 
GATGATTGTG TCCGCGACGT GGCCGACGAT CGGGAGCAGA TATTGGCGCA CTTTGATGAC 
CCAGGACCCG ATCCAGGAGT TCCGCAAGGA GGCGGAGATG GAGCATCGGG CAGTTCGTCC 
GTTGGCACCG GTTCGCCGGA CATCTTTCGC GATCCCACCA ACACGGAGGC GCCCACCTGT 
CCGCGGGATC TCTCCACGCC ACACATCGAG GTCACCAGCA CCACATCGGG ACCGATGGCT 
GGACTCGGAG TTGGACTGAT GGTGCGTCGC AGCAGTGATC CCAATCTGCT GGCCTCGCTG 
AAGGCAGAGG GCAGTAACAA ACGCTGGTCG GCGGCGGCTC CCCACTACGC TGGCGGGGAT 
TCGCCGGAGC GCCTGTTTCT TGACAAGGCC GGTGGCCAGT TATCGCCACA GTGGGAGGAG 
GACGACGATC CCAGTCATCA GCTAAAGGAA CAGCTGCTAC ATCAACAGCA GCCCCATGCT 
GCTAATGGAG GCTCCTCCTC CGGTAACCAT CAGCCGTTTG CCCGATCCGG TCGCCTGTCG 
ATGCAATTTC TTGGCGATGG CAATGGCTAC AAGTGGATGG AGGCAGCCGA GAAACTTCAG 
AATCAGCCGC CAGCCCAGCA GACATATCAG CAGGGTTCTC ATCATGCTGG TCATGGTCAA 
AACGGTGCCT ACTCCAGCAA GTCCTTGCCC AGGGAGAGCA AGCGAAAGGA GCCCTTGGGA 
CAGGCATATG AATCCATCAG GGAGAAGGAT GGTGAAATGC TGCTGATCAT CAACGAGTAC 
GGCAGTCCGC TGGGACTAAC TGCTCTGCCG GACAAGGAGC ATGGCGGTGG ACTGTTGGTG 
CAACATGTGG AGCCGGGCAG CCGAGCCGAG AGAGGACGAC TGCGTCGTGA TGATCGCATC 
TTGGAGATCA ATGGAATCAA GCTAATAGGA CTCACCGAAT CGCAGGTTCA GGAGCAACTG 
CGGCGGGCTT TGGAGAGCTC AGAGTTGAGA GTTCGAGTGC TGCGCGGTGA TCGCAATCGC 
CGCCAACAGC GTGACTCTAA GGTGGCCGAG ATGGTGGAGG TGGCTACGGT GTCACCCACC 
CGCAAACCAC ATGCTGCTCC GGTGGGCACC TCGCTGCAGG TGGCCAATAC CCGTAAACTG 
GGCAGGAAAA TCGAAATTAT GCTCAAGAAG GGACCCAACG GTCTGGGCTT TTCGGTCACA 
ACACGCGATA ATCCCGCCGG TGGCCACTGT CCCATCTACA TCAAGAATAT CCTGCCACGA 
GGTGCGGCCA TCGAGGATGG ACGCCTGAAG CCCGGCGATC GTTTGCTCGA GGTGGATGGC 
ACTCCAATGA CTGGTAAAAC ACAAACGGAT GTGGTGGCAA TCTTGAGGGG CATGCCAGCG 
GGAGCAACCG TAAGGATTGT GGTCTCCCGC CAGCAGGAGT TGGCGGAGCA GGCTGACCAG 
CCGGCGGAGA AAAGTGCTGG TGTGGCGGTG GCGCCCTCAG TTGCTCCACC AGCTGTTCCT 
GCAGCTGCAG CTCCAGCGCC TCCCATTCCG GTACAAAAAT CCAGCAGCGC ACGTTCTCTG 
TTCACTCATC AGCAGCAATC GCAGCTCAAC GAATCTCAGC ACTTTATTGA TGCGGGCAGC 
GAGTCGGCGG CTTCAAATGA CAGCCTGCCG CCCAGCAGCA ACAGTTGGCA CTCCCGCGAG 
GAGCTGACCC TGCACATTCC CGTACACGAC ACCGAAAAGG CCGGACTGGG TGTCAGTGTG 
AAGGGAAAGA CGTGCTCGAA TCTGAACGCT TCCGGATCGA GTGCCTCCAG CGGCAGCAAT 
GGACTGATGA AGCATGACGG TGACTTGGGT ATATTCGTGA AGAATGTAAT TCATGGCGGT 
GCTGCATCCC GCGATGGTCG CTTGCGGATG AATGATCAAC TGCTGAGCGT GAATGGAGTA 
TCGCTGCGTG GGCAAAACAA TGCCGAGGCC ATGGAGACAC TACGTCGGGC AATGGTCAAC 
ACGCCCGGCA AACATCCGGG AACCATAACC CTGCTGGTAG GCCGCAAGAT CTTGCGATCG 
GCCAGTTCCA GTGACATTCT GGACCACAGT AACAGTCACA GTCATAGCCA TAGCAATAGT 
AGCGGTGGCA GCAATTCAAA TGGTAGCGGC AATAACAACA ATAGCAGCTC GAATGCCAGC 
GATAATTCTG GAGCCACGGT CATATATTTG AGTCCGGAGA AGAGGGAGCA GCGCTGCAAT 
GGCGGCGGAG GTGGTGGCAG TGCTGGCAAT GAGATGAATA GATGGAGCAA TCCCGTTTTG 
GATCGCCTAA CCGGTGGCAT TTGCTCCTCG AATTCAGCGC AGCCATCCTC TCAGCAGTCT 
CACCAGCAGC AGCCGCATCC TTCGCAGCAG CAACAGCAGC AACGTCGCCT GCCTGCAGCG 
CCCGTTTGCA GCAGTGCAGC TCTGAGAAAC GAGAGCTACT ACATGGCCAC CAATGACAAC 
TGGTCGCCGG CGCAGATGCA CTTAATGACT GCTCATGGCA ACACGGCGCT GCTTATCGAG 
GACGATGCCG AGCCGATGTC TCCAACACTA CCGGCACGTC CGCATGATGG GCAGCACTGC 
AACACGAGCA GTGCTAATCC ATCGCAGAAC TTGGCCGTCG GCAATCAGGG GCCACCCATA 
AATACCGTGC CTGGTACTCC GTCGACATCC AGCAACTTTG ATGCCACCTA CTCCTCGCAA 
CTGAGCTTGG AGACAAACTC GGGCGTGGAG CATTTCTCGC GCGATGCTTT GGGACGACGC 
AGCATCTCTG AGAAGCACCA TGCGGCGCTG GATGCCCGCG AAACTGGCAC CTATCAGCGG 
AATAAGAAGT TACGCGAGGA GCGCGAACGC GAGCGTCGCA TTCAGCTGAC CAAATCGGCT 
GTGTATGGTG GTTCCATTGA GTCCCTTACG GCTCGCATAG CCAGTGCTAA TGCCCAGTTT 
TCGGGATATA AACATGCCAA GACTGCATCC AGTATCGAGC AAAGAGAGAC GCAGCAGCAA 
TTGGCCGCTG CGGAGGCGGA GGCAAGGGAT CAGCTGGGCG ATCTGGGTCC ATCGCTGGGC 
ATGAAGAAGT CCTCGTCGTT GGAGTCGCTC CAGACTATGG TGCAGGAGCT GCAGATGTCG 
GATGAGCCGC GTGGTCATCA GGCGTTGCGC GCACCGCGTG GGCGTGGCAG GGAGGACAGT 
CTGCGGGCGG CGGTGGTCAG CGAACCGGAT GCGAGCAAGC CCCGCAAGAC CTGGCTTTTG 
GAGGATGGCG ATCACGAGGG TGGTTTTGCC TCGCAGCGCA ATGGACCGTT CCAGAGTTCC 
CTGAACGATG GCAAACACGG CTGCAAGTCG TCGCGGGCCA AGAAGCCAAG CATACTGCGC 
GGTATCGGTC ACATGTTCCG CTTTGGCAAG AATCGCAAGG ATGGCGTGGT GCCAGTGGAT 
AACTATGCGG TAAACATTTC GCCCCCTACA TCGGTGGTTT CCACAGCCAC ATCGCCGCAG 
CTGCAGCAGC AGCAACAGCA GCAGTTGCAG CAACATCAGC AGCAACAACA GATACCAACC 
GCTGCATTGG CTGCTCTGGA GAGAAATGGC AAACCGCCGG CGTATCAGCC ACCGCCACCG 
CTGCCTGCTC CAAATGGAGT GGGTAGCAAT GGTATCCATC AGAACGACAT CTTCAACCAT 
CGCTATCAGC ATTATGCCAA CTACGAGGAC CTCCACCAGC AGCACCAGCA ACACCAGATT 
AGTGGAGGCG ATTCCACCAC ATCGATTTCG GAAACGCTTT CGGAGTCAAC ACTCGAGTGC 
ATGCGACAGC AGGTCATCCG TCAGCGCATC AAGGTCGAGG CGGAAAGTCG CCGCCATCAG 
CATTACCATT CGCAGCGCAG TGCCCGCTCG CAGGATGTGA GCATGCACTC GACGAGCTCC 
GGATCCCAGC CAGGATCCCT GGCTCAGCCG CAAGCGCAAT CAAACGGCGT TCGTCCCATG 
AGCAGTTACT ACGAGTACGA GACGGTTCAA CAGCAACGGG TGGGCAGCAT CAAGCATAGC 
CACAGCAGCA GCGCCACGTC GTCCTCCTCG TCGCCCATCA ATGTGCCGCA TTGGAAGGCG 
GCAGCCATGA ATGGCTACTC GCCAGCCAGC CTGAATAGCA GTGCCCGGAG TCGAGGTCCG 
TTTGTGACGC AGGTGACCAT ACGGGAGCAG AGCAGTGGCG GCATACCCGC CCACCTGCTG 
CAGCAACATC AGCAGCAGCA ACTCCAGCAG CAGCAGCCCA CCTACCAGAC TGTTCAGAAG 
ATGTCAGGAC CATCGCAATA TGGTAGTGCC GCCGGTTCCC AGCCACACGC CTCCAAGGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036283.1
CDS	4958..9520
Translation	MFDVPPKCPALANKLGGLFGWRHTYKVAAKQEGIPHGQLHKSYSLTLPKRPPSPSTYSCVKPDSWVTVTHLQTQSGILDPDDCVRDVADDREQILAHFDDPGPDPGVPQGGGDGASGSSSVGTGSPDIFRDPTNTEAPTCPRDLSTPHIEVTSTTSGPMAGLGVGLMVRRSSDPNLLASLKAEGSNKRWSAAAPHYAGGDSPERLFLDKAGGQLSPQWEEDDDPSHQLKEQLLHQQQPHAANGGSSSGNHQPFARSGRLSMQFLGDGNGYKWMEAAEKLQNQPPAQQTYQQGSHHAGHGQNGAYSSKSLPRESKRKEPLGQAYESIREKDGEMLLIINEYGSPLGLTALPDKEHGGGLLVQHVEPGSRAERGRLRRDDRILEINGIKLIGLTESQVQEQLRRALESSELRVRVLRGDRNRRQQRDSKVAEMVEVATVSPTRKPHAAPVGTSLQVANTRKLGRKIEIMLKKGPNGLGFSVTTRDNPAGGHCPIYIKNILPRGAAIEDGRLKPGDRLLEVDGTPMTGKTQTDVVAILRGMPAGATVRIVVSRQQELAEQADQPAEKSAGVAVAPSVAPPAVPAAAAPAPPIPVQKSSSARSLFTHQQQSQLNESQHFIDAGSESAASNDSLPPSSNSWHSREELTLHIPVHDTEKAGLGVSVKGKTCSNLNASGSSASSGSNGLMKHDGDLGIFVKNVIHGGAASRDGRLRMNDQLLSVNGVSLRGQNNAEAMETLRRAMVNTPGKHPGTITLLVGRKILRSASSSDILDHSNSHSHSHSNSSGGSNSNGSGNNNNSSSNASDNSGATVIYLSPEKREQRCNGGGGGGSAGNEMNRWSNPVLDRLTGGICSSNSAQPSSQQSHQQQPHPSQQQQQQRRLPAAPVCSSAALRNESYYMATNDNWSPAQMHLMTAHGNTALLIEDDAEPMSPTLPARPHDGQHCNTSSANPSQNLAVGNQGPPINTVPGTPSTSSNFDATYSSQLSLETNSGVEHFSRDALGRRSISEKHHAALDARETGTYQRNKKLREERERERRIQLTKSAVYGGSIESLTARIASANAQFSGYKHAKTASSIEQRETQQQLAAAEAEARDQLGDLGPSLGMKKSSSLESLQTMVQELQMSDEPRGHQALRAPRGRGREDSLRAAVVSEPDASKPRKTWLLEDGDHEGGFASQRNGPFQSSLNDGKHGCKSSRAKKPSILRGIGHMFRFGKNRKDGVVPVDNYAVNISPPTSVVSTATSPQLQQQQQQQLQQHQQQQQIPTAALAALERNGKPPAYQPPPPLPAPNGVGSNGIHQNDIFNHRYQHYANYEDLHQQHQQHQISGGDSTTSISETLSESTLECMRQQVIRQRIKVEAESRRHQHYHSQRSARSQDVSMHSTSSGSQPGSLAQPQAQSNGVRPMSSYYEYETVQQQRVGSIKHSHSSSATSSSSSPINVPHWKAAAMNGYSPASLNSSARSRGPFVTQVTIREQSSGGIPAHLLQQHQQQQLQQQQPTYQTVQKMSGPSQYGSAAGSQPHASKV

Target ORF information:

RefSeq Version	NM_001042818.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster bazooka (baz), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001042818.4

ORF Insert Sequence:

ATGTTCGACG TACCGCCAAA GTGTCCTGCG TTGGCCAACA AGCTGGGCGG TCTCTTTGGG 
TGGCGACACA CCTACAAGGT GGCCGCCAAG CAGGAGGGGA TTCCCCATGG GCAGCTACAC 
AAGTCCTACT CCCTCACGCT GCCCAAGCGA CCGCCTTCGC CATCGACCTA TTCGTGTGTG 
AAGCCCGATT CCTGGGTGAC CGTGACGCAT CTCCAGACAC AGTCGGGCAT CCTCGATCCG 
GATGATTGTG TCCGCGACGT GGCCGACGAT CGGGAGCAGA TATTGGCGCA CTTTGATGAC 
CCAGGACCCG ATCCAGGAGT TCCGCAAGGA GGCGGAGATG GAGCATCGGG CAGTTCGTCC 
GTTGGCACCG GTTCGCCGGA CATCTTTCGC GATCCCACCA ACACGGAGGC GCCCACCTGT 
CCGCGGGATC TCTCCACGCC ACACATCGAG GTCACCAGCA CCACATCGGG ACCGATGGCT 
GGACTCGGAG TTGGACTGAT GGTGCGTCGC AGCAGTGATC CCAATCTGCT GGCCTCGCTG 
AAGGCAGAGG GCAGTAACAA ACGCTGGTCG GCGGCGGCTC CCCACTACGC TGGCGGGGAT 
TCGCCGGAGC GCCTGTTTCT TGACAAGGCC GGTGGCCAGT TATCGCCACA GTGGGAGGAG 
GACGACGATC CCAGTCATCA GCTAAAGGAA CAGCTGCTAC ATCAACAGCA GCCCCATGCT 
GCTAATGGAG GCTCCTCCTC CGGTAACCAT CAGCCGTTTG CCCGATCCGG TCGCCTGTCG 
ATGCAATTTC TTGGCGATGG CAATGGCTAC AAGTGGATGG AGGCAGCCGA GAAACTTCAG 
AATCAGCCGC CAGCCCAGCA GACATATCAG CAGGGTTCTC ATCATGCTGG TCATGGTCAA 
AACGGTGCCT ACTCCAGCAA GTCCTTGCCC AGGGAGAGCA AGCGAAAGGA GCCCTTGGGA 
CAGGCATATG AATCCATCAG GGAGAAGGAT GGTGAAATGC TGCTGATCAT CAACGAGTAC 
GGCAGTCCGC TGGGACTAAC TGCTCTGCCG GACAAGGAGC ATGGCGGTGG ACTGTTGGTG 
CAACATGTGG AGCCGGGCAG CCGAGCCGAG AGAGGACGAC TGCGTCGTGA TGATCGCATC 
TTGGAGATCA ATGGAATCAA GCTAATAGGA CTCACCGAAT CGCAGGTTCA GGAGCAACTG 
CGGCGGGCTT TGGAGAGCTC AGAGTTGAGA GTTCGAGTGC TGCGCGGTGA TCGCAATCGC 
CGCCAACAGC GTGACTCTAA GGTGGCCGAG ATGGTGGAGG TGGCTACGGT GTCACCCACC 
CGCAAACCAC ATGCTGCTCC GGTGGGCACC TCGCTGCAGG TGGCCAATAC CCGTAAACTG 
GGCAGGAAAA TCGAAATTAT GCTCAAGAAG GGACCCAACG GTCTGGGCTT TTCGGTCACA 
ACACGCGATA ATCCCGCCGG TGGCCACTGT CCCATCTACA TCAAGAATAT CCTGCCACGA 
GGTGCGGCCA TCGAGGATGG ACGCCTGAAG CCCGGCGATC GTTTGCTCGA GGTGGATGGC 
ACTCCAATGA CTGGTAAAAC ACAAACGGAT GTGGTGGCAA TCTTGAGGGG CATGCCAGCG 
GGAGCAACCG TAAGGATTGT GGTCTCCCGC CAGCAGGAGT TGGCGGAGCA GGCTGACCAG 
CCGGCGGAGA AAAGTGCTGG TGTGGCGGTG GCGCCCTCAG TTGCTCCACC AGCTGTTCCT 
GCAGCTGCAG CTCCAGCGCC TCCCATTCCG GTACAAAAAT CCAGCAGCGC ACGTTCTCTG 
TTCACTCATC AGCAGCAATC GCAGCTCAAC GAATCTCAGC ACTTTATTGA TGCGGGCAGC 
GAGTCGGCGG CTTCAAATGA CAGCCTGCCG CCCAGCAGCA ACAGTTGGCA CTCCCGCGAG 
GAGCTGACCC TGCACATTCC CGTACACGAC ACCGAAAAGG CCGGACTGGG TGTCAGTGTG 
AAGGGAAAGA CGTGCTCGAA TCTGAACGCT TCCGGATCGA GTGCCTCCAG CGGCAGCAAT 
GGACTGATGA AGCATGACGG TGACTTGGGT ATATTCGTGA AGAATGTAAT TCATGGCGGT 
GCTGCATCCC GCGATGGTCG CTTGCGGATG AATGATCAAC TGCTGAGCGT GAATGGAGTA 
TCGCTGCGTG GGCAAAACAA TGCCGAGGCC ATGGAGACAC TACGTCGGGC AATGGTCAAC 
ACGCCCGGCA AACATCCGGG AACCATAACC CTGCTGGTAG GCCGCAAGAT CTTGCGATCG 
GCCAGTTCCA GTGACATTCT GGACCACAGT AACAGTCACA GTCATAGCCA TAGCAATAGT 
AGCGGTGGCA GCAATTCAAA TGGTAGCGGC AATAACAACA ATAGCAGCTC GAATGCCAGC 
GATAATTCTG GAGCCACGGT CATATATTTG AGTCCGGAGA AGAGGGAGCA GCGCTGCAAT 
GGCGGCGGAG GTGGTGGCAG TGCTGGCAAT GAGATGAATA GATGGAGCAA TCCCGTTTTG 
GATCGCCTAA CCGGTGGCAT TTGCTCCTCG AATTCAGCGC AGCCATCCTC TCAGCAGTCT 
CACCAGCAGC AGCCGCATCC TTCGCAGCAG CAACAGCAGC AACGTCGCCT GCCTGCAGCG 
CCCGTTTGCA GCAGTGCAGC TCTGAGAAAC GAGAGCTACT ACATGGCCAC CAATGACAAC 
TGGTCGCCGG CGCAGATGCA CTTAATGACT GCTCATGGCA ACACGGCGCT GCTTATCGAG 
GACGATGCCG AGCCGATGTC TCCAACACTA CCGGCACGTC CGCATGATGG GCAGCACTGC 
AACACGAGCA GTGCTAATCC ATCGCAGAAC TTGGCCGTCG GCAATCAGGG GCCACCCATA 
AATACCGTGC CTGGTACTCC GTCGACATCC AGCAACTTTG ATGCCACCTA CTCCTCGCAA 
CTGAGCTTGG AGACAAACTC GGGCGTGGAG CATTTCTCGC GCGATGCTTT GGGACGACGC 
AGCATCTCTG AGAAGCACCA TGCGGCGCTG GATGCCCGCG AAACTGGCAC CTATCAGCGG 
AATAAGAAGT TACGCGAGGA GCGCGAACGC GAGCGTCGCA TTCAGCTGAC CAAATCGGCT 
GTGTATGGTG GTTCCATTGA GTCCCTTACG GCTCGCATAG CCAGTGCTAA TGCCCAGTTT 
TCGGGATATA AACATGCCAA GACTGCATCC AGTATCGAGC AAAGAGAGAC GCAGCAGCAA 
TTGGCCGCTG CGGAGGCGGA GGCAAGGGAT CAGCTGGGCG ATCTGGGTCC ATCGCTGGGC 
ATGAAGAAGT CCTCGTCGTT GGAGTCGCTC CAGACTATGG TGCAGGAGCT GCAGATGTCG 
GATGAGCCGC GTGGTCATCA GGCGTTGCGC GCACCGCGTG GGCGTGGCAG GGAGGACAGT 
CTGCGGGCGG CGGTGGTCAG CGAACCGGAT GCGAGCAAGC CCCGCAAGAC CTGGCTTTTG 
GAGGATGGCG ATCACGAGGG TGGTTTTGCC TCGCAGCGCA ATGGACCGTT CCAGAGTTCC 
CTGAACGATG GCAAACACGG CTGCAAGTCG TCGCGGGCCA AGAAGCCAAG CATACTGCGC 
GGTATCGGTC ACATGTTCCG CTTTGGCAAG AATCGCAAGG ATGGCGTGGT GCCAGTGGAT 
AACTATGCGG TAAACATTTC GCCCCCTACA TCGGTGGTTT CCACAGCCAC ATCGCCGCAG 
CTGCAGCAGC AGCAACAGCA GCAGTTGCAG CAACATCAGC AGCAACAACA GATACCAACC 
GCTGCATTGG CTGCTCTGGA GAGAAATGGC AAACCGCCGG CGTATCAGCC ACCGCCACCG 
CTGCCTGCTC CAAATGGAGT GGGTAGCAAT GGTATCCATC AGAACGACAT CTTCAACCAT 
CGCTATCAGC ATTATGCCAA CTACGAGGAC CTCCACCAGC AGCACCAGCA ACACCAGATT 
AGTGGAGGCG ATTCCACCAC ATCGATTTCG GAAACGCTTT CGGAGTCAAC ACTCGAGTGC 
ATGCGACAGC AGGTCATCCG TCAGCGCATC AAGGTCGAGG CGGAAAGTCG CCGCCATCAG 
CATTACCATT CGCAGCGCAG TGCCCGCTCG CAGGATGTGA GCATGCACTC GACGAGCTCC 
GGATCCCAGC CAGGATCCCT GGCTCAGCCG CAAGCGCAAT CAAACGGCGT TCGTCCCATG 
AGCAGTTACT ACGAGTACGA GACGGTTCAA CAGCAACGGG TGGGCAGCAT CAAGCATAGC 
CACAGCAGCA GCGCCACGTC GTCCTCCTCG TCGCCCATCA ATGTGCCGCA TTGGAAGGCG 
GCAGCCATGA ATGGCTACTC GCCAGCCAGC CTGAATAGCA GTGCCCGGAG TCGAGGTCCG 
TTTGTGACGC AGGTGACCAT ACGGGAGCAG AGCAGTGGCG GCATACCCGC CCACCTGCTG 
CAGCAACATC AGCAGCAGCA ACTCCAGCAG CAGCAGCCCA CCTACCAGAC TGTTCAGAAG 
ATGTCAGGAC CATCGCAATA TGGTAGTGCC GCCGGTTCCC AGCCACACGC CTCCAAGGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

baz ( NM_001298436.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285365.1 Drosophila melanogaster bazooka (baz), transcript variant D, mRNA.

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CloneID	OFa21513
Clone ID Related Accession (Same CDS sequence)	NM_001298436.2
Accession Version	NM_001298436.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	bazooka, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Dec 13, 2016 this sequence version replaced NM_001298436.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCGACG TACCGCCAAA GTGTCCTGCG TTGGCCAACA AGCTGGGCGG TCTCTTTGGG 
TGGCGACACA CCTACAAGGT GGCCGCCAAG CAGGAGGGGA TTCCCCATGG GCAGCTACAC 
AAGTCCTACT CCCTCACGCT GCCCAAGCGA CCGCCTTCGC CATCGACCTA TTCGTGTGTG 
AAGCCCGATT CCTGGGTGAC CGTGACGCAT CTCCAGACAC AGTCGGGCAT CCTCGATCCG 
GATGATTGTG TCCGCGACGT GGCCGACGAT CGGGAGCAGA TATTGGCGCA CTTTGATGAC 
CCAGGACCCG ATCCAGGAGT TCCGCAAGGA GGCGGAGATG GAGCATCGGG CAGTTCGTCC 
GTTGGCACCG GTTCGCCGGA CATCTTTCGC GATCCCACCA ACACGGAGGC GCCCACCTGT 
CCGCGGGATC TCTCCACGCC ACACATCGAG GTCACCAGCA CCACATCGGG ACCGATGGCT 
GGACTCGGAG TTGGACTGAT GGTGCGTCGC AGCAGTGATC CCAATCTGCT GGCCTCGCTG 
AAGGCAGAGG GCAGTAACAA ACGCTGGTCG GCGGCGGCTC CCCACTACGC TGGCGGGGAT 
TCGCCGGAGC GCCTGTTTCT TGACAAGGCC GGTGGCCAGT TATCGCCACA GTGGGAGGAG 
GACGACGATC CCAGTCATCA GCTAAAGGAA CAGCTGCTAC ATCAACAGCA GCCCCATGCT 
GCTAATGGAG GCTCCTCCTC CGGTAACCAT CAGCCGTTTG CCCGATCCGG TCGCCTGTCG 
ATGCAATTTC TTGGCGATGG CAATGGCTAC AAGTGGATGG AGGCAGCCGA GAAACTTCAG 
AATCAGCCGC CAGCCCAGCA GACATATCAG CAGGGTTCTC ATCATGCTGG TCATGGTCAA 
AACGGTGCCT ACTCCAGCAA GTCCTTGCCC AGGGAGAGCA AGCGAAAGGA GCCCTTGGGA 
CAGGCATATG AATCCATCAG GGAGAAGGAT GGTGAAATGC TGCTGATCAT CAACGAGTAC 
GGCAGTCCGC TGGGACTAAC TGCTCTGCCG GACAAGGAGC ATGGCGGTGG ACTGTTGGTG 
CAACATGTGG AGCCGGGCAG CCGAGCCGAG AGAGGACGAC TGCGTCGTGA TGATCGCATC 
TTGGAGATCA ATGGAATCAA GCTAATAGGA CTCACCGAAT CGCAGGTTCA GGAGCAACTG 
CGGCGGGCTT TGGAGAGCTC AGAGTTGAGA GTTCGAGTGC TGCGCGGTGA TCGCAATCGC 
CGCCAACAGC GTGACTCTAA GGTGGCCGAG ATGGTGGAGG TGGCTACGGT GTCACCCACC 
CGCAAACCAC ATGCTGCTCC GGTGGGCACC TCGCTGCAGG TGGCCAATAC CCGTAAACTG 
GGCAGGAAAA TCGAAATTAT GCTCAAGAAG GGACCCAACG GTCTGGGCTT TTCGGTCACA 
ACACGCGATA ATCCCGCCGG TGGCCACTGT CCCATCTACA TCAAGAATAT CCTGCCACGA 
GGTGCGGCCA TCGAGGATGG ACGCCTGAAG CCCGGCGATC GTTTGCTCGA GGTGGATGGC 
ACTCCAATGA CTGGTAAAAC ACAAACGGAT GTGGTGGCAA TCTTGAGGGG CATGCCAGCG 
GGAGCAACCG TAAGGATTGT GGTCTCCCGC CAGCAGGAGT TGGCGGAGCA GGCTGACCAG 
CCGGCGGAGA AAAGTGCTGG TGTGGCGGTG GCGCCCTCAG TTGCTCCACC AGCTGTTCCT 
GCAGCTGCAG CTCCAGCGCC TCCCATTCCG GTACAAAAAT CCAGCAGCGC ACGTTCTCTG 
TTCACTCATC AGCAGCAATC GCAGCTCAAC GAATCTCAGC ACTTTATTGA TGCGGGCAGC 
GAGTCGGCGG CTTCAAATGA CAGCCTGCCG CCCAGCAGCA ACAGTTGGCA CTCCCGCGAG 
GAGCTGACCC TGCACATTCC CGTACACGAC ACCGAAAAGG CCGGACTGGG TGTCAGTGTG 
AAGGGAAAGA CGTGCTCGAA TCTGAACGCT TCCGGATCGA GTGCCTCCAG CGGCAGCAAT 
GGACTGATGA AGCATGACGG TGACTTGGGT ATATTCGTGA AGAATGTAAT TCATGGCGGT 
GCTGCATCCC GCGATGGTCG CTTGCGGATG AATGATCAAC TGCTGAGCGT GAATGGAGTA 
TCGCTGCGTG GGCAAAACAA TGCCGAGGCC ATGGAGACAC TACGTCGGGC AATGGTCAAC 
ACGCCCGGCA AACATCCGGG AACCATAACC CTGCTGGTAG GCCGCAAGAT CTTGCGATCG 
GCCAGTTCCA GTGACATTCT GGACCACAGT AACAGTCACA GTCATAGCCA TAGCAATAGT 
AGCGGTGGCA GCAATTCAAA TGGTAGCGGC AATAACAACA ATAGCAGCTC GAATGCCAGC 
GATAATTCTG GAGCCACGGT CATATATTTG AGTCCGGAGA AGAGGGAGCA GCGCTGCAAT 
GGCGGCGGAG GTGGTGGCAG TGCTGGCAAT GAGATGAATA GATGGAGCAA TCCCGTTTTG 
GATCGCCTAA CCGGTGGCAT TTGCTCCTCG AATTCAGCGC AGCCATCCTC TCAGCAGTCT 
CACCAGCAGC AGCCGCATCC TTCGCAGCAG CAACAGCAGC AACGTCGCCT GCCTGCAGCG 
CCCGTTTGCA GCAGTGCAGC TCTGAGAAAC GAGAGCTACT ACATGGCCAC CAATGACAAC 
TGGTCGCCGG CGCAGATGCA CTTAATGACT GCTCATGGCA ACACGGCGCT GCTTATCGAG 
GACGATGCCG AGCCGATGTC TCCAACACTA CCGGCACGTC CGCATGATGG GCAGCACTGC 
AACACGAGCA GTGCTAATCC ATCGCAGAAC TTGGCCGTCG GCAATCAGGG GCCACCCATA 
AATACCGTGC CTGGTACTCC GTCGACATCC AGCAACTTTG ATGCCACCTA CTCCTCGCAA 
CTGAGCTTGG AGACAAACTC GGGCGTGGAG CATTTCTCGC GCGATGCTTT GGGACGACGC 
AGCATCTCTG AGAAGCACCA TGCGGCGCTG GATGCCCGCG AAACTGGCAC CTATCAGCGG 
AATAAGAAGT TACGCGAGGA GCGCGAACGC GAGCGTCGCA TTCAGCTGAC CAAATCGGCT 
GTGTATGGTG GTTCCATTGA GTCCCTTACG GCTCGCATAG CCAGTGCTAA TGCCCAGTTT 
TCGGGATATA AACATGCCAA GACTGCATCC AGTATCGAGC AAAGAGAGAC GCAGCAGCAA 
TTGGCCGCTG CGGAGGCGGA GGCAAGGGAT CAGCTGGGCG ATCTGGGTCC ATCGCTGGGC 
ATGAAGAAGT CCTCGTCGTT GGAGTCGCTC CAGACTATGG TGCAGGAGCT GCAGATGTCG 
GATGAGCCGC GTGGTCATCA GGCGTTGCGC GCACCGCGTG GGCGTGGCAG GGAGGACAGT 
CTGCGGGCGG CGGTGGTCAG CGAACCGGAT GCGAGCAAGC CCCGCAAGAC CTGGCTTTTG 
GAGGATGGCG ATCACGAGGG TGGTTTTGCC TCGCAGCGCA ATGGACCGTT CCAGAGTTCC 
CTGAACGATG GCAAACACGG CTGCAAGTCG TCGCGGGCCA AGAAGCCAAG CATACTGCGC 
GGTATCGGTC ACATGTTCCG CTTTGGCAAG AATCGCAAGG ATGGCGTGGT GCCAGTGGAT 
AACTATGCGG TAAACATTTC GCCCCCTACA TCGGTGGTTT CCACAGCCAC ATCGCCGCAG 
CTGCAGCAGC AGCAACAGCA GCAGTTGCAG CAACATCAGC AGCAACAACA GATACCAACC 
GCTGCATTGG CTGCTCTGGA GAGAAATGGC AAACCGCCGG CGTATCAGCC ACCGCCACCG 
CTGCCTGCTC CAAATGGAGT GGGTAGCAAT GGTATCCATC AGAACGACAT CTTCAACCAT 
CGCTATCAGC ATTATGCCAA CTACGAGGAC CTCCACCAGC AGCACCAGCA ACACCAGATT 
AGTCGCCGCC ATCAGCATTA CCATTCGCAG CGCAGTGCCC GCTCGCAGGA TGTGAGCATG 
CACTCGACGA GCTCCGGATC CCAGCCAGGA TCCCTGGCTC AGCCGCAAGC GCAATCAAAC 
GGCGTTCGTC CCATGAGCAG TTACTACGAG TACGAGACGG TTCAACAGCA ACGGGTGGGC 
AGCATCAAGC ATAGCCACAG CAGCAGCGCC ACGTCGTCCT CCTCGTCGCC CATCAATGTG 
CCGCATTGGA AGGCGGCAGC CATGAATGGC TACTCGCCAG CCAGCCTGAA TAGCAGTGCC 
CGGAGTCGAG GTCCGTTTGT GACGCAGGTG ACCATACGGG AGCAGAGCAG TGGCGGCATA 
CCCGCCCACC TGCTGCAGCA ACATCAGCAG CAGCAACTCC AGCAGCAGCA GCCCACCTAC 
CAGACTGTTC AGAAGATGTC AGGACCATCG CAATATGGTA GTGCCGCCGG TTCCCAGCCA 
CACGCCTCCA AGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285365.1
CDS	4958..9415
Translation	MFDVPPKCPALANKLGGLFGWRHTYKVAAKQEGIPHGQLHKSYSLTLPKRPPSPSTYSCVKPDSWVTVTHLQTQSGILDPDDCVRDVADDREQILAHFDDPGPDPGVPQGGGDGASGSSSVGTGSPDIFRDPTNTEAPTCPRDLSTPHIEVTSTTSGPMAGLGVGLMVRRSSDPNLLASLKAEGSNKRWSAAAPHYAGGDSPERLFLDKAGGQLSPQWEEDDDPSHQLKEQLLHQQQPHAANGGSSSGNHQPFARSGRLSMQFLGDGNGYKWMEAAEKLQNQPPAQQTYQQGSHHAGHGQNGAYSSKSLPRESKRKEPLGQAYESIREKDGEMLLIINEYGSPLGLTALPDKEHGGGLLVQHVEPGSRAERGRLRRDDRILEINGIKLIGLTESQVQEQLRRALESSELRVRVLRGDRNRRQQRDSKVAEMVEVATVSPTRKPHAAPVGTSLQVANTRKLGRKIEIMLKKGPNGLGFSVTTRDNPAGGHCPIYIKNILPRGAAIEDGRLKPGDRLLEVDGTPMTGKTQTDVVAILRGMPAGATVRIVVSRQQELAEQADQPAEKSAGVAVAPSVAPPAVPAAAAPAPPIPVQKSSSARSLFTHQQQSQLNESQHFIDAGSESAASNDSLPPSSNSWHSREELTLHIPVHDTEKAGLGVSVKGKTCSNLNASGSSASSGSNGLMKHDGDLGIFVKNVIHGGAASRDGRLRMNDQLLSVNGVSLRGQNNAEAMETLRRAMVNTPGKHPGTITLLVGRKILRSASSSDILDHSNSHSHSHSNSSGGSNSNGSGNNNNSSSNASDNSGATVIYLSPEKREQRCNGGGGGGSAGNEMNRWSNPVLDRLTGGICSSNSAQPSSQQSHQQQPHPSQQQQQQRRLPAAPVCSSAALRNESYYMATNDNWSPAQMHLMTAHGNTALLIEDDAEPMSPTLPARPHDGQHCNTSSANPSQNLAVGNQGPPINTVPGTPSTSSNFDATYSSQLSLETNSGVEHFSRDALGRRSISEKHHAALDARETGTYQRNKKLREERERERRIQLTKSAVYGGSIESLTARIASANAQFSGYKHAKTASSIEQRETQQQLAAAEAEARDQLGDLGPSLGMKKSSSLESLQTMVQELQMSDEPRGHQALRAPRGRGREDSLRAAVVSEPDASKPRKTWLLEDGDHEGGFASQRNGPFQSSLNDGKHGCKSSRAKKPSILRGIGHMFRFGKNRKDGVVPVDNYAVNISPPTSVVSTATSPQLQQQQQQQLQQHQQQQQIPTAALAALERNGKPPAYQPPPPLPAPNGVGSNGIHQNDIFNHRYQHYANYEDLHQQHQQHQISRRHQHYHSQRSARSQDVSMHSTSSGSQPGSLAQPQAQSNGVRPMSSYYEYETVQQQRVGSIKHSHSSSATSSSSSPINVPHWKAAAMNGYSPASLNSSARSRGPFVTQVTIREQSSGGIPAHLLQQHQQQQLQQQQPTYQTVQKMSGPSQYGSAAGSQPHASKV

Target ORF information:

RefSeq Version	NM_001298436.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster bazooka (baz), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298436.2

ORF Insert Sequence:

ATGTTCGACG TACCGCCAAA GTGTCCTGCG TTGGCCAACA AGCTGGGCGG TCTCTTTGGG 
TGGCGACACA CCTACAAGGT GGCCGCCAAG CAGGAGGGGA TTCCCCATGG GCAGCTACAC 
AAGTCCTACT CCCTCACGCT GCCCAAGCGA CCGCCTTCGC CATCGACCTA TTCGTGTGTG 
AAGCCCGATT CCTGGGTGAC CGTGACGCAT CTCCAGACAC AGTCGGGCAT CCTCGATCCG 
GATGATTGTG TCCGCGACGT GGCCGACGAT CGGGAGCAGA TATTGGCGCA CTTTGATGAC 
CCAGGACCCG ATCCAGGAGT TCCGCAAGGA GGCGGAGATG GAGCATCGGG CAGTTCGTCC 
GTTGGCACCG GTTCGCCGGA CATCTTTCGC GATCCCACCA ACACGGAGGC GCCCACCTGT 
CCGCGGGATC TCTCCACGCC ACACATCGAG GTCACCAGCA CCACATCGGG ACCGATGGCT 
GGACTCGGAG TTGGACTGAT GGTGCGTCGC AGCAGTGATC CCAATCTGCT GGCCTCGCTG 
AAGGCAGAGG GCAGTAACAA ACGCTGGTCG GCGGCGGCTC CCCACTACGC TGGCGGGGAT 
TCGCCGGAGC GCCTGTTTCT TGACAAGGCC GGTGGCCAGT TATCGCCACA GTGGGAGGAG 
GACGACGATC CCAGTCATCA GCTAAAGGAA CAGCTGCTAC ATCAACAGCA GCCCCATGCT 
GCTAATGGAG GCTCCTCCTC CGGTAACCAT CAGCCGTTTG CCCGATCCGG TCGCCTGTCG 
ATGCAATTTC TTGGCGATGG CAATGGCTAC AAGTGGATGG AGGCAGCCGA GAAACTTCAG 
AATCAGCCGC CAGCCCAGCA GACATATCAG CAGGGTTCTC ATCATGCTGG TCATGGTCAA 
AACGGTGCCT ACTCCAGCAA GTCCTTGCCC AGGGAGAGCA AGCGAAAGGA GCCCTTGGGA 
CAGGCATATG AATCCATCAG GGAGAAGGAT GGTGAAATGC TGCTGATCAT CAACGAGTAC 
GGCAGTCCGC TGGGACTAAC TGCTCTGCCG GACAAGGAGC ATGGCGGTGG ACTGTTGGTG 
CAACATGTGG AGCCGGGCAG CCGAGCCGAG AGAGGACGAC TGCGTCGTGA TGATCGCATC 
TTGGAGATCA ATGGAATCAA GCTAATAGGA CTCACCGAAT CGCAGGTTCA GGAGCAACTG 
CGGCGGGCTT TGGAGAGCTC AGAGTTGAGA GTTCGAGTGC TGCGCGGTGA TCGCAATCGC 
CGCCAACAGC GTGACTCTAA GGTGGCCGAG ATGGTGGAGG TGGCTACGGT GTCACCCACC 
CGCAAACCAC ATGCTGCTCC GGTGGGCACC TCGCTGCAGG TGGCCAATAC CCGTAAACTG 
GGCAGGAAAA TCGAAATTAT GCTCAAGAAG GGACCCAACG GTCTGGGCTT TTCGGTCACA 
ACACGCGATA ATCCCGCCGG TGGCCACTGT CCCATCTACA TCAAGAATAT CCTGCCACGA 
GGTGCGGCCA TCGAGGATGG ACGCCTGAAG CCCGGCGATC GTTTGCTCGA GGTGGATGGC 
ACTCCAATGA CTGGTAAAAC ACAAACGGAT GTGGTGGCAA TCTTGAGGGG CATGCCAGCG 
GGAGCAACCG TAAGGATTGT GGTCTCCCGC CAGCAGGAGT TGGCGGAGCA GGCTGACCAG 
CCGGCGGAGA AAAGTGCTGG TGTGGCGGTG GCGCCCTCAG TTGCTCCACC AGCTGTTCCT 
GCAGCTGCAG CTCCAGCGCC TCCCATTCCG GTACAAAAAT CCAGCAGCGC ACGTTCTCTG 
TTCACTCATC AGCAGCAATC GCAGCTCAAC GAATCTCAGC ACTTTATTGA TGCGGGCAGC 
GAGTCGGCGG CTTCAAATGA CAGCCTGCCG CCCAGCAGCA ACAGTTGGCA CTCCCGCGAG 
GAGCTGACCC TGCACATTCC CGTACACGAC ACCGAAAAGG CCGGACTGGG TGTCAGTGTG 
AAGGGAAAGA CGTGCTCGAA TCTGAACGCT TCCGGATCGA GTGCCTCCAG CGGCAGCAAT 
GGACTGATGA AGCATGACGG TGACTTGGGT ATATTCGTGA AGAATGTAAT TCATGGCGGT 
GCTGCATCCC GCGATGGTCG CTTGCGGATG AATGATCAAC TGCTGAGCGT GAATGGAGTA 
TCGCTGCGTG GGCAAAACAA TGCCGAGGCC ATGGAGACAC TACGTCGGGC AATGGTCAAC 
ACGCCCGGCA AACATCCGGG AACCATAACC CTGCTGGTAG GCCGCAAGAT CTTGCGATCG 
GCCAGTTCCA GTGACATTCT GGACCACAGT AACAGTCACA GTCATAGCCA TAGCAATAGT 
AGCGGTGGCA GCAATTCAAA TGGTAGCGGC AATAACAACA ATAGCAGCTC GAATGCCAGC 
GATAATTCTG GAGCCACGGT CATATATTTG AGTCCGGAGA AGAGGGAGCA GCGCTGCAAT 
GGCGGCGGAG GTGGTGGCAG TGCTGGCAAT GAGATGAATA GATGGAGCAA TCCCGTTTTG 
GATCGCCTAA CCGGTGGCAT TTGCTCCTCG AATTCAGCGC AGCCATCCTC TCAGCAGTCT 
CACCAGCAGC AGCCGCATCC TTCGCAGCAG CAACAGCAGC AACGTCGCCT GCCTGCAGCG 
CCCGTTTGCA GCAGTGCAGC TCTGAGAAAC GAGAGCTACT ACATGGCCAC CAATGACAAC 
TGGTCGCCGG CGCAGATGCA CTTAATGACT GCTCATGGCA ACACGGCGCT GCTTATCGAG 
GACGATGCCG AGCCGATGTC TCCAACACTA CCGGCACGTC CGCATGATGG GCAGCACTGC 
AACACGAGCA GTGCTAATCC ATCGCAGAAC TTGGCCGTCG GCAATCAGGG GCCACCCATA 
AATACCGTGC CTGGTACTCC GTCGACATCC AGCAACTTTG ATGCCACCTA CTCCTCGCAA 
CTGAGCTTGG AGACAAACTC GGGCGTGGAG CATTTCTCGC GCGATGCTTT GGGACGACGC 
AGCATCTCTG AGAAGCACCA TGCGGCGCTG GATGCCCGCG AAACTGGCAC CTATCAGCGG 
AATAAGAAGT TACGCGAGGA GCGCGAACGC GAGCGTCGCA TTCAGCTGAC CAAATCGGCT 
GTGTATGGTG GTTCCATTGA GTCCCTTACG GCTCGCATAG CCAGTGCTAA TGCCCAGTTT 
TCGGGATATA AACATGCCAA GACTGCATCC AGTATCGAGC AAAGAGAGAC GCAGCAGCAA 
TTGGCCGCTG CGGAGGCGGA GGCAAGGGAT CAGCTGGGCG ATCTGGGTCC ATCGCTGGGC 
ATGAAGAAGT CCTCGTCGTT GGAGTCGCTC CAGACTATGG TGCAGGAGCT GCAGATGTCG 
GATGAGCCGC GTGGTCATCA GGCGTTGCGC GCACCGCGTG GGCGTGGCAG GGAGGACAGT 
CTGCGGGCGG CGGTGGTCAG CGAACCGGAT GCGAGCAAGC CCCGCAAGAC CTGGCTTTTG 
GAGGATGGCG ATCACGAGGG TGGTTTTGCC TCGCAGCGCA ATGGACCGTT CCAGAGTTCC 
CTGAACGATG GCAAACACGG CTGCAAGTCG TCGCGGGCCA AGAAGCCAAG CATACTGCGC 
GGTATCGGTC ACATGTTCCG CTTTGGCAAG AATCGCAAGG ATGGCGTGGT GCCAGTGGAT 
AACTATGCGG TAAACATTTC GCCCCCTACA TCGGTGGTTT CCACAGCCAC ATCGCCGCAG 
CTGCAGCAGC AGCAACAGCA GCAGTTGCAG CAACATCAGC AGCAACAACA GATACCAACC 
GCTGCATTGG CTGCTCTGGA GAGAAATGGC AAACCGCCGG CGTATCAGCC ACCGCCACCG 
CTGCCTGCTC CAAATGGAGT GGGTAGCAAT GGTATCCATC AGAACGACAT CTTCAACCAT 
CGCTATCAGC ATTATGCCAA CTACGAGGAC CTCCACCAGC AGCACCAGCA ACACCAGATT 
AGTCGCCGCC ATCAGCATTA CCATTCGCAG CGCAGTGCCC GCTCGCAGGA TGTGAGCATG 
CACTCGACGA GCTCCGGATC CCAGCCAGGA TCCCTGGCTC AGCCGCAAGC GCAATCAAAC 
GGCGTTCGTC CCATGAGCAG TTACTACGAG TACGAGACGG TTCAACAGCA ACGGGTGGGC 
AGCATCAAGC ATAGCCACAG CAGCAGCGCC ACGTCGTCCT CCTCGTCGCC CATCAATGTG 
CCGCATTGGA AGGCGGCAGC CATGAATGGC TACTCGCCAG CCAGCCTGAA TAGCAGTGCC 
CGGAGTCGAG GTCCGTTTGT GACGCAGGTG ACCATACGGG AGCAGAGCAG TGGCGGCATA 
CCCGCCCACC TGCTGCAGCA ACATCAGCAG CAGCAACTCC AGCAGCAGCA GCCCACCTAC 
CAGACTGTTC AGAAGATGTC AGGACCATCG CAATATGGTA GTGCCGCCGG TTCCCAGCCA 
CACGCCTCCA AGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

baz ( NM_001298435.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285364.1 Drosophila melanogaster bazooka (baz), transcript variant C, mRNA.

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CloneID	OFa21514
Clone ID Related Accession (Same CDS sequence)	NM_001298435.2
Accession Version	NM_001298435.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	bazooka, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Dec 13, 2016 this sequence version replaced NM_001298435.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGGTCA CCGTCTGCTT CGGCGACGTT CGCATTCTGG TTCCCTGTGG TTCCGGCGAA 
CTCCTCGTCC GCGATCTCGT CAAGGAGGCC ACGCGACGCT ACATCAAGGC GGCCGGCAAG 
CCCGATTCCT GGGTGACCGT GACGCATCTC CAGACACAGT CGGGCATCCT CGATCCGGAT 
GATTGTGTCC GCGACGTGGC CGACGATCGG GAGCAGATAT TGGCGCACTT TGATGACCCA 
GGACCCGATC CAGGAGTTCC GCAAGGAGGC GGAGATGGAG CATCGGGCAG TTCGTCCGTT 
GGCACCGGTT CGCCGGACAT CTTTCGCGAT CCCACCAACA CGGAGGCGCC CACCTGTCCG 
CGGGATCTCT CCACGCCACA CATCGAGGTC ACCAGCACCA CATCGGGACC GATGGCTGGA 
CTCGGAGTTG GACTGATGGT GCGTCGCAGC AGTGATCCCA ATCTGCTGGC CTCGCTGAAG 
GCAGAGGGCA GTAACAAACG CTGGTCGGCG GCGGCTCCCC ACTACGCTGG CGGGGATTCG 
CCGGAGCGCC TGTTTCTTGA CAAGGCCGGT GGCCAGTTAT CGCCACAGTG GGAGGAGGAC 
GACGATCCCA GTCATCAGCT AAAGGAACAG CTGCTACATC AACAGCAGCC CCATGCTGCT 
AATGGAGGCT CCTCCTCCGG TAACCATCAG CCGTTTGCCC GATCCGGTCG CCTGTCGATG 
CAATTTCTTG GCGATGGCAA TGGCTACAAG TGGATGGAGG CAGCCGAGAA ACTTCAGAAT 
CAGCCGCCAG CCCAGCAGAC ATATCAGCAG GGTTCTCATC ATGCTGGTCA TGGTCAAAAC 
GGTGCCTACT CCAGCAAGTC CTTGCCCAGG GAGAGCAAGC GAAAGGAGCC CTTGGGACAG 
GCATATGAAT CCATCAGGGA GAAGGATGGT GAAATGCTGC TGATCATCAA CGAGTACGGC 
AGTCCGCTGG GACTAACTGC TCTGCCGGAC AAGGAGCATG GCGGTGGACT GTTGGTGCAA 
CATGTGGAGC CGGGCAGCCG AGCCGAGAGA GGACGACTGC GTCGTGATGA TCGCATCTTG 
GAGATCAATG GAATCAAGCT AATAGGACTC ACCGAATCGC AGGTTCAGGA GCAACTGCGG 
CGGGCTTTGG AGAGCTCAGA GTTGAGAGTT CGAGTGCTGC GCGGTGATCG CAATCGCCGC 
CAACAGCGTG ACTCTAAGGT GGCCGAGATG GTGGAGGTGG CTACGGTGTC ACCCACCCGC 
AAACCACATG CTGCTCCGGT GGGCACCTCG CTGCAGGTGG CCAATACCCG TAAACTGGGC 
AGGAAAATCG AAATTATGCT CAAGAAGGGA CCCAACGGTC TGGGCTTTTC GGTCACAACA 
CGCGATAATC CCGCCGGTGG CCACTGTCCC ATCTACATCA AGAATATCCT GCCACGAGGT 
GCGGCCATCG AGGATGGACG CCTGAAGCCC GGCGATCGTT TGCTCGAGGT GGATGGCACT 
CCAATGACTG GTAAAACACA AACGGATGTG GTGGCAATCT TGAGGGGCAT GCCAGCGGGA 
GCAACCGTAA GGATTGTGGT CTCCCGCCAG CAGGAGTTGG CGGAGCAGGC TGACCAGCCG 
GCGGAGAAAA GTGCTGGTGT GGCGGTGGCG CCCTCAGTTG CTCCACCAGC TGTTCCTGCA 
GCTGCAGCTC CAGCGCCTCC CATTCCGGTA CAAAAATCCA GCAGCGCACG TTCTCTGTTC 
ACTCATCAGC AGCAATCGCA GCTCAACGAA TCTCAGCACT TTATTGATGC GGGCAGCGAG 
TCGGCGGCTT CAAATGACAG CCTGCCGCCC AGCAGCAACA GTTGGCACTC CCGCGAGGAG 
CTGACCCTGC ACATTCCCGT ACACGACACC GAAAAGGCCG GACTGGGTGT CAGTGTGAAG 
GGAAAGACGT GCTCGAATCT GAACGCTTCC GGATCGAGTG CCTCCAGCGG CAGCAATGGA 
CTGATGAAGC ATGACGGTGA CTTGGGTATA TTCGTGAAGA ATGTAATTCA TGGCGGTGCT 
GCATCCCGCG ATGGTCGCTT GCGGATGAAT GATCAACTGC TGAGCGTGAA TGGAGTATCG 
CTGCGTGGGC AAAACAATGC CGAGGCCATG GAGACACTAC GTCGGGCAAT GGTCAACACG 
CCCGGCAAAC ATCCGGGAAC CATAACCCTG CTGGTAGGCC GCAAGATCTT GCGATCGGCC 
AGTTCCAGTG ACATTCTGGA CCACAGTAAC AGTCACAGTC ATAGCCATAG CAATAGTAGC 
GGTGGCAGCA ATTCAAATGG TAGCGGCAAT AACAACAATA GCAGCTCGAA TGCCAGCGAT 
AATTCTGGAG CCACGGTCAT ATATTTGAGT CCGGAGAAGA GGGAGCAGCG CTGCAATGGC 
GGCGGAGGTG GTGGCAGTGC TGGCAATGAG ATGAATAGAT GGAGCAATCC CGTTTTGGAT 
CGCCTAACCG GTGGCATTTG CTCCTCGAAT TCAGCGCAGC CATCCTCTCA GCAGTCTCAC 
CAGCAGCAGC CGCATCCTTC GCAGCAGCAA CAGCAGCAAC GTCGCCTGCC TGCAGCGCCC 
GTTTGCAGCA GTGCAGCTCT GAGAAACGAG AGCTACTACA TGGCCACCAA TGACAACTGG 
TCGCCGGCGC AGATGCACTT AATGACTGCT CATGGCAACA CGGCGCTGCT TATCGAGGAC 
GATGCCGAGC CGATGTCTCC AACACTACCG GCACGTCCGC ATGATGGGCA GCACTGCAAC 
ACGAGCAGTG CTAATCCATC GCAGAACTTG GCCGTCGGCA ATCAGGGGCC ACCCATAAAT 
ACCGTGCCTG GTACTCCGTC GACATCCAGC AACTTTGATG CCACCTACTC CTCGCAACTG 
AGCTTGGAGA CAAACTCGGG CGTGGAGCAT TTCTCGCGCG ATGCTTTGGG ACGACGCAGC 
ATCTCTGAGA AGCACCATGC GGCGCTGGAT GCCCGCGAAA CTGGCACCTA TCAGCGGAAT 
AAGAAGTTAC GCGAGGAGCG CGAACGCGAG CGTCGCATTC AGCTGACCAA ATCGGCTGTG 
TATGGTGGTT CCATTGAGTC CCTTACGGCT CGCATAGCCA GTGCTAATGC CCAGTTTTCG 
GGATATAAAC ATGCCAAGAC TGCATCCAGT ATCGAGCAAA GAGAGACGCA GCAGCAATTG 
GCCGCTGCGG AGGCGGAGGC AAGGGATCAG CTGGGCGATC TGGGTCCATC GCTGGGCATG 
AAGAAGTCCT CGTCGTTGGA GTCGCTCCAG ACTATGGTGC AGGAGCTGCA GATGTCGGAT 
GAGCCGCGTG GTCATCAGGC GTTGCGCGCA CCGCGTGGGC GTGGCAGGGA GGACAGTCTG 
CGGGCGGCGG TGGTCAGCGA ACCGGATGCG AGCAAGCCCC GCAAGACCTG GCTTTTGGAG 
GATGGCGATC ACGAGGGTGG TTTTGCCTCG CAGCGCAATG GACCGTTCCA GAGTTCCCTG 
AACGATGGCA AACACGGCTG CAAGTCGTCG CGGGCCAAGA AGCCAAGCAT ACTGCGCGGT 
ATCGGTCACA TGTTCCGCTT TGGCAAGAAT CGCAAGGATG GCGTGGTGCC AGTGGATAAC 
TATGCGGTAA ACATTTCGCC CCCTACATCG GTGGTTTCCA CAGCCACATC GCCGCAGCTG 
CAGCAGCAGC AACAGCAGCA GTTGCAGCAA CATCAGCAGC AACAACAGAT ACCAACCGCT 
GCATTGGCTG CTCTGGAGAG AAATGGCAAA CCGCCGGCGT ATCAGCCACC GCCACCGCTG 
CCTGCTCCAA ATGGAGTGGG TAGCAATGGT ATCCATCAGA ACGACATCTT CAACCATCGC 
TATCAGCATT ATGCCAACTA CGAGGACCTC CACCAGCAGC ACCAGCAACA CCAGATTAGT 
GGAGGCGATT CCACCACATC GATTTCGGAA ACGCTTTCGG AGTCAACACT CGAGTGCATG 
CGACAGCAGG TCATCCGTCA GCGCATCAAG GTCGAGGCGG AAAGTCGCCG CCATCAGCAT 
TACCATTCGC AGCGCAGTGC CCGCTCGCAG GATGTGAGCA TGCACTCGAC GAGCTCCGGA 
TCCCAGCCAG GATCCCTGGC TCAGCCGCAA GCGCAATCAA ACGGCGTTCG TCCCATGAGC 
AGTTACTACG AGTACGAGAC GGTTCAACAG CAACGGGTGG GCAGCATCAA GCATAGCCAC 
AGCAGCAGCG CCACGTCGTC CTCCTCGTCG CCCATCAATG TGCCGCATTG GAAGGCGGCA 
GCCATGAATG GCTACTCGCC AGCCAGCCTG AATAGCAGTG CCCGGAGTCG AGGTCCGTTT 
GTGACGCAGG TGACCATACG GGAGCAGAGC AGTGGCGGCA TACCCGCCCA CCTGCTGCAG 
CAACATCAGC AGCAGCAACT CCAGCAGCAG CAGCCCACCT ACCAGACTGT TCAGAAGATG 
TCAGGACCAT CGCAATATGG TAGTGCCGCC GGTTCCCAGC CACACGCCTC CAAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285364.1
CDS	1067..5566
Translation	MKVTVCFGDVRILVPCGSGELLVRDLVKEATRRYIKAAGKPDSWVTVTHLQTQSGILDPDDCVRDVADDREQILAHFDDPGPDPGVPQGGGDGASGSSSVGTGSPDIFRDPTNTEAPTCPRDLSTPHIEVTSTTSGPMAGLGVGLMVRRSSDPNLLASLKAEGSNKRWSAAAPHYAGGDSPERLFLDKAGGQLSPQWEEDDDPSHQLKEQLLHQQQPHAANGGSSSGNHQPFARSGRLSMQFLGDGNGYKWMEAAEKLQNQPPAQQTYQQGSHHAGHGQNGAYSSKSLPRESKRKEPLGQAYESIREKDGEMLLIINEYGSPLGLTALPDKEHGGGLLVQHVEPGSRAERGRLRRDDRILEINGIKLIGLTESQVQEQLRRALESSELRVRVLRGDRNRRQQRDSKVAEMVEVATVSPTRKPHAAPVGTSLQVANTRKLGRKIEIMLKKGPNGLGFSVTTRDNPAGGHCPIYIKNILPRGAAIEDGRLKPGDRLLEVDGTPMTGKTQTDVVAILRGMPAGATVRIVVSRQQELAEQADQPAEKSAGVAVAPSVAPPAVPAAAAPAPPIPVQKSSSARSLFTHQQQSQLNESQHFIDAGSESAASNDSLPPSSNSWHSREELTLHIPVHDTEKAGLGVSVKGKTCSNLNASGSSASSGSNGLMKHDGDLGIFVKNVIHGGAASRDGRLRMNDQLLSVNGVSLRGQNNAEAMETLRRAMVNTPGKHPGTITLLVGRKILRSASSSDILDHSNSHSHSHSNSSGGSNSNGSGNNNNSSSNASDNSGATVIYLSPEKREQRCNGGGGGGSAGNEMNRWSNPVLDRLTGGICSSNSAQPSSQQSHQQQPHPSQQQQQQRRLPAAPVCSSAALRNESYYMATNDNWSPAQMHLMTAHGNTALLIEDDAEPMSPTLPARPHDGQHCNTSSANPSQNLAVGNQGPPINTVPGTPSTSSNFDATYSSQLSLETNSGVEHFSRDALGRRSISEKHHAALDARETGTYQRNKKLREERERERRIQLTKSAVYGGSIESLTARIASANAQFSGYKHAKTASSIEQRETQQQLAAAEAEARDQLGDLGPSLGMKKSSSLESLQTMVQELQMSDEPRGHQALRAPRGRGREDSLRAAVVSEPDASKPRKTWLLEDGDHEGGFASQRNGPFQSSLNDGKHGCKSSRAKKPSILRGIGHMFRFGKNRKDGVVPVDNYAVNISPPTSVVSTATSPQLQQQQQQQLQQHQQQQQIPTAALAALERNGKPPAYQPPPPLPAPNGVGSNGIHQNDIFNHRYQHYANYEDLHQQHQQHQISGGDSTTSISETLSESTLECMRQQVIRQRIKVEAESRRHQHYHSQRSARSQDVSMHSTSSGSQPGSLAQPQAQSNGVRPMSSYYEYETVQQQRVGSIKHSHSSSATSSSSSPINVPHWKAAAMNGYSPASLNSSARSRGPFVTQVTIREQSSGGIPAHLLQQHQQQQLQQQQPTYQTVQKMSGPSQYGSAAGSQPHASKV

Target ORF information:

RefSeq Version	NM_001298435.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster bazooka (baz), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298435.2

ORF Insert Sequence:

ATGAAGGTCA CCGTCTGCTT CGGCGACGTT CGCATTCTGG TTCCCTGTGG TTCCGGCGAA 
CTCCTCGTCC GCGATCTCGT CAAGGAGGCC ACGCGACGCT ACATCAAGGC GGCCGGCAAG 
CCCGATTCCT GGGTGACCGT GACGCATCTC CAGACACAGT CGGGCATCCT CGATCCGGAT 
GATTGTGTCC GCGACGTGGC CGACGATCGG GAGCAGATAT TGGCGCACTT TGATGACCCA 
GGACCCGATC CAGGAGTTCC GCAAGGAGGC GGAGATGGAG CATCGGGCAG TTCGTCCGTT 
GGCACCGGTT CGCCGGACAT CTTTCGCGAT CCCACCAACA CGGAGGCGCC CACCTGTCCG 
CGGGATCTCT CCACGCCACA CATCGAGGTC ACCAGCACCA CATCGGGACC GATGGCTGGA 
CTCGGAGTTG GACTGATGGT GCGTCGCAGC AGTGATCCCA ATCTGCTGGC CTCGCTGAAG 
GCAGAGGGCA GTAACAAACG CTGGTCGGCG GCGGCTCCCC ACTACGCTGG CGGGGATTCG 
CCGGAGCGCC TGTTTCTTGA CAAGGCCGGT GGCCAGTTAT CGCCACAGTG GGAGGAGGAC 
GACGATCCCA GTCATCAGCT AAAGGAACAG CTGCTACATC AACAGCAGCC CCATGCTGCT 
AATGGAGGCT CCTCCTCCGG TAACCATCAG CCGTTTGCCC GATCCGGTCG CCTGTCGATG 
CAATTTCTTG GCGATGGCAA TGGCTACAAG TGGATGGAGG CAGCCGAGAA ACTTCAGAAT 
CAGCCGCCAG CCCAGCAGAC ATATCAGCAG GGTTCTCATC ATGCTGGTCA TGGTCAAAAC 
GGTGCCTACT CCAGCAAGTC CTTGCCCAGG GAGAGCAAGC GAAAGGAGCC CTTGGGACAG 
GCATATGAAT CCATCAGGGA GAAGGATGGT GAAATGCTGC TGATCATCAA CGAGTACGGC 
AGTCCGCTGG GACTAACTGC TCTGCCGGAC AAGGAGCATG GCGGTGGACT GTTGGTGCAA 
CATGTGGAGC CGGGCAGCCG AGCCGAGAGA GGACGACTGC GTCGTGATGA TCGCATCTTG 
GAGATCAATG GAATCAAGCT AATAGGACTC ACCGAATCGC AGGTTCAGGA GCAACTGCGG 
CGGGCTTTGG AGAGCTCAGA GTTGAGAGTT CGAGTGCTGC GCGGTGATCG CAATCGCCGC 
CAACAGCGTG ACTCTAAGGT GGCCGAGATG GTGGAGGTGG CTACGGTGTC ACCCACCCGC 
AAACCACATG CTGCTCCGGT GGGCACCTCG CTGCAGGTGG CCAATACCCG TAAACTGGGC 
AGGAAAATCG AAATTATGCT CAAGAAGGGA CCCAACGGTC TGGGCTTTTC GGTCACAACA 
CGCGATAATC CCGCCGGTGG CCACTGTCCC ATCTACATCA AGAATATCCT GCCACGAGGT 
GCGGCCATCG AGGATGGACG CCTGAAGCCC GGCGATCGTT TGCTCGAGGT GGATGGCACT 
CCAATGACTG GTAAAACACA AACGGATGTG GTGGCAATCT TGAGGGGCAT GCCAGCGGGA 
GCAACCGTAA GGATTGTGGT CTCCCGCCAG CAGGAGTTGG CGGAGCAGGC TGACCAGCCG 
GCGGAGAAAA GTGCTGGTGT GGCGGTGGCG CCCTCAGTTG CTCCACCAGC TGTTCCTGCA 
GCTGCAGCTC CAGCGCCTCC CATTCCGGTA CAAAAATCCA GCAGCGCACG TTCTCTGTTC 
ACTCATCAGC AGCAATCGCA GCTCAACGAA TCTCAGCACT TTATTGATGC GGGCAGCGAG 
TCGGCGGCTT CAAATGACAG CCTGCCGCCC AGCAGCAACA GTTGGCACTC CCGCGAGGAG 
CTGACCCTGC ACATTCCCGT ACACGACACC GAAAAGGCCG GACTGGGTGT CAGTGTGAAG 
GGAAAGACGT GCTCGAATCT GAACGCTTCC GGATCGAGTG CCTCCAGCGG CAGCAATGGA 
CTGATGAAGC ATGACGGTGA CTTGGGTATA TTCGTGAAGA ATGTAATTCA TGGCGGTGCT 
GCATCCCGCG ATGGTCGCTT GCGGATGAAT GATCAACTGC TGAGCGTGAA TGGAGTATCG 
CTGCGTGGGC AAAACAATGC CGAGGCCATG GAGACACTAC GTCGGGCAAT GGTCAACACG 
CCCGGCAAAC ATCCGGGAAC CATAACCCTG CTGGTAGGCC GCAAGATCTT GCGATCGGCC 
AGTTCCAGTG ACATTCTGGA CCACAGTAAC AGTCACAGTC ATAGCCATAG CAATAGTAGC 
GGTGGCAGCA ATTCAAATGG TAGCGGCAAT AACAACAATA GCAGCTCGAA TGCCAGCGAT 
AATTCTGGAG CCACGGTCAT ATATTTGAGT CCGGAGAAGA GGGAGCAGCG CTGCAATGGC 
GGCGGAGGTG GTGGCAGTGC TGGCAATGAG ATGAATAGAT GGAGCAATCC CGTTTTGGAT 
CGCCTAACCG GTGGCATTTG CTCCTCGAAT TCAGCGCAGC CATCCTCTCA GCAGTCTCAC 
CAGCAGCAGC CGCATCCTTC GCAGCAGCAA CAGCAGCAAC GTCGCCTGCC TGCAGCGCCC 
GTTTGCAGCA GTGCAGCTCT GAGAAACGAG AGCTACTACA TGGCCACCAA TGACAACTGG 
TCGCCGGCGC AGATGCACTT AATGACTGCT CATGGCAACA CGGCGCTGCT TATCGAGGAC 
GATGCCGAGC CGATGTCTCC AACACTACCG GCACGTCCGC ATGATGGGCA GCACTGCAAC 
ACGAGCAGTG CTAATCCATC GCAGAACTTG GCCGTCGGCA ATCAGGGGCC ACCCATAAAT 
ACCGTGCCTG GTACTCCGTC GACATCCAGC AACTTTGATG CCACCTACTC CTCGCAACTG 
AGCTTGGAGA CAAACTCGGG CGTGGAGCAT TTCTCGCGCG ATGCTTTGGG ACGACGCAGC 
ATCTCTGAGA AGCACCATGC GGCGCTGGAT GCCCGCGAAA CTGGCACCTA TCAGCGGAAT 
AAGAAGTTAC GCGAGGAGCG CGAACGCGAG CGTCGCATTC AGCTGACCAA ATCGGCTGTG 
TATGGTGGTT CCATTGAGTC CCTTACGGCT CGCATAGCCA GTGCTAATGC CCAGTTTTCG 
GGATATAAAC ATGCCAAGAC TGCATCCAGT ATCGAGCAAA GAGAGACGCA GCAGCAATTG 
GCCGCTGCGG AGGCGGAGGC AAGGGATCAG CTGGGCGATC TGGGTCCATC GCTGGGCATG 
AAGAAGTCCT CGTCGTTGGA GTCGCTCCAG ACTATGGTGC AGGAGCTGCA GATGTCGGAT 
GAGCCGCGTG GTCATCAGGC GTTGCGCGCA CCGCGTGGGC GTGGCAGGGA GGACAGTCTG 
CGGGCGGCGG TGGTCAGCGA ACCGGATGCG AGCAAGCCCC GCAAGACCTG GCTTTTGGAG 
GATGGCGATC ACGAGGGTGG TTTTGCCTCG CAGCGCAATG GACCGTTCCA GAGTTCCCTG 
AACGATGGCA AACACGGCTG CAAGTCGTCG CGGGCCAAGA AGCCAAGCAT ACTGCGCGGT 
ATCGGTCACA TGTTCCGCTT TGGCAAGAAT CGCAAGGATG GCGTGGTGCC AGTGGATAAC 
TATGCGGTAA ACATTTCGCC CCCTACATCG GTGGTTTCCA CAGCCACATC GCCGCAGCTG 
CAGCAGCAGC AACAGCAGCA GTTGCAGCAA CATCAGCAGC AACAACAGAT ACCAACCGCT 
GCATTGGCTG CTCTGGAGAG AAATGGCAAA CCGCCGGCGT ATCAGCCACC GCCACCGCTG 
CCTGCTCCAA ATGGAGTGGG TAGCAATGGT ATCCATCAGA ACGACATCTT CAACCATCGC 
TATCAGCATT ATGCCAACTA CGAGGACCTC CACCAGCAGC ACCAGCAACA CCAGATTAGT 
GGAGGCGATT CCACCACATC GATTTCGGAA ACGCTTTCGG AGTCAACACT CGAGTGCATG 
CGACAGCAGG TCATCCGTCA GCGCATCAAG GTCGAGGCGG AAAGTCGCCG CCATCAGCAT 
TACCATTCGC AGCGCAGTGC CCGCTCGCAG GATGTGAGCA TGCACTCGAC GAGCTCCGGA 
TCCCAGCCAG GATCCCTGGC TCAGCCGCAA GCGCAATCAA ACGGCGTTCG TCCCATGAGC 
AGTTACTACG AGTACGAGAC GGTTCAACAG CAACGGGTGG GCAGCATCAA GCATAGCCAC 
AGCAGCAGCG CCACGTCGTC CTCCTCGTCG CCCATCAATG TGCCGCATTG GAAGGCGGCA 
GCCATGAATG GCTACTCGCC AGCCAGCCTG AATAGCAGTG CCCGGAGTCG AGGTCCGTTT 
GTGACGCAGG TGACCATACG GGAGCAGAGC AGTGGCGGCA TACCCGCCCA CCTGCTGCAG 
CAACATCAGC AGCAGCAACT CCAGCAGCAG CAGCCCACCT ACCAGACTGT TCAGAAGATG 
TCAGGACCAT CGCAATATGG TAGTGCCGCC GGTTCCCAGC CACACGCCTC CAAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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baz cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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