RpS5a cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	RpS5a
Entrez Gene ID	32700
Full Name	Ribosomal protein S5a
Synonyms	CG8922,Dmel\CG8922,M(1)15D,M(1)15d,M(1)34i28,M(1)o,M[[o]],RS5,RS5A_DROME,Rp S5a,RpS5,S5a,rpS5,rps5
General protein information	Preferred Names Ribosomal protein S5a Names CG8922 gene product from transcript CG8922-RA CG8922-PA CG8922-PB CG8922-PC CG8922-PD Minute(1)15D RpS5a-PA RpS5a-PB RpS5a-PC RpS5a-PD minute 1(o) ribosomal protein S5 ribosomal protein S5 gene ribosomal protein S5a transcript e
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 15E5-15E7\|1-56.6 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001298431.1	NP_001285360.1	ribosomal protein S5a, isoform B
NM_001298432.1	NP_001285361.1	ribosomal protein S5a, isoform C
NM_078658.3	NP_523382.1	ribosomal protein S5a, isoform A
NM_001298433.1	NP_001285362.1	ribosomal protein S5a, isoform D

Pathways from BioSystems

Homologies

Bos taurus (cattle)	RPS5	NP_001015531.1
Mus musculus (house mouse)	Rps5	NP_033121.2
Eremothecium gossypii	AGOS_ABR171W	NP_983119.1
Arabidopsis thaliana (thale cress)	RPS5A	NP_187800.1
Arabidopsis thaliana (thale cress)	RPS5B	NP_181264.1
Pan troglodytes (chimpanzee)	RPS5	XP_001147137.2
	RPS5	XP_001097103.1
Schizosaccharomyces pombe (fission yeast)	rps5	NP_594279.1
Magnaporthe oryzae (rice blast fungus)	MGG_06658	XP_003709365.1
Neurospora crassa	NCU09475	XP_963230.2
Canis lupus familiaris (dog)	RPS5	XP_533568.1
Xenopus tropicalis (tropical clawed frog)	rps5	NP_001016992.1
Rattus norvegicus (Norway rat)	Rps5	NP_001264172.1
Drosophila melanogaster (fruit fly)	RpS5a	NP_523382.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008329	XP_555129.1
Caenorhabditis elegans (roundworm)	rps-5	NP_502077.1
Schizosaccharomyces pombe (fission yeast)	rps502	NP_594212.1
Oryza sativa (rice)	Os01g0100700	NP_001041738.1
Oryza sativa (rice)	Os11g0482000	NP_001067900.1
Homo sapiens (human)	RPS5	NP_001000.2
Danio rerio (zebrafish)	rps5	NP_775339.1
Saccharomyces cerevisiae (baker's yeast)	RPS5	NP_012657.1
Kluyveromyces lactis	KLLA0D10659g	XP_453536.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following RpS5a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RpS5a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01874	NM_078658.3 Latest version!	Drosophila melanogaster ribosomal protein S5a, transcript variant A (RpS5a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa01874	NM_001298431.1 Latest version!	Drosophila melanogaster ribosomal protein S5a, transcript variant B (RpS5a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa01874	NM_001298433.1 Latest version!	Drosophila melanogaster ribosomal protein S5a, transcript variant D (RpS5a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa01874	NM_001298432.1 Latest version!	Drosophila melanogaster ribosomal protein S5a, transcript variant C (RpS5a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RpS5a ( NM_001298431.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285360.1 Drosophila melanogaster ribosomal protein S5a, transcript variant B (RpS5a), mRNA.

Price & Availability↑

CloneID	OFa01874
Clone ID Related Accession (Same CDS sequence)	NM_001298431.1 , NM_001298432.1 , NM_078658.3 , NM_001298433.1
Accession Version	NM_001298431.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ribosomal protein S5a, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGAAG TTGCTGAAAA CGTGGTGGAG ACCTTCGAGG AGCCAGCGGC ACCTATGGAA 
GCCGAGGTGG CCGAGACGAT CCTGGAGACA AATGTGGTGT CCACCACTGA GCTGCCGGAG 
ATCAAGCTGT TCGGCCGCTG GTCTTGCGAC GATGTCACCG TTAACGACAT CTCTCTGCAG 
GATTACATCT CGGTGAAGGA GAAGTTTGCC CGCTATCTTC CCCATTCCGC CGGACGTTAT 
GCCGCCAAGC GTTTCCGCAA GGCCCAGTGC CCCATTGTGG AGCGTTTGAC CTGCTCCCTG 
ATGATGAAGG GTCGCAACAA CGGCAAGAAG CTGATGGCCT GCCGCATCGT CAAGCACTCG 
TTCGAGATCA TTCATCTGCT CACCGGGGAG AACCCTCTGC AGATCCTGGT CAGCGCCATC 
ATCAACTCGG GACCCCGTGA GGACTCCACC CGTATTGGAC GTGCCGGTAC CGTCCGTCGC 
CAGGCCGTCG ATGTGTCGCC CCTGCGTCGC GTCAACCAGG CTATCTGGCT GCTGTGCACT 
GGAGCTCGTG AGGCTGCCTT CAGGAACATC AAGACCATCG CCGAGTGCCT GGCTGATGAG 
CTGATCAACG CTGCTAAGGG ATCTTCCAAC TCGTACGCCA TCAAGAAGAA GGATGAGTTG 
GAGCGTGTCG CCAAGTCCAA CCGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285360.1
CDS	210..896
Translation	MAEVAENVVETFEEPAAPMEAEVAETILETNVVSTTELPEIKLFGRWSCDDVTVNDISLQDYISVKEKFARYLPHSAGRYAAKRFRKAQCPIVERLTCSLMMKGRNNGKKLMACRIVKHSFEIIHLLTGENPLQILVSAIINSGPREDSTRIGRAGTVRRQAVDVSPLRRVNQAIWLLCTGAREAAFRNIKTIAECLADELINAAKGSSNSYAIKKKDELERVAKSNR

Target ORF information:

RefSeq Version	NM_001298431.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ribosomal protein S5a, transcript variant B (RpS5a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298431.1

ORF Insert Sequence:

ATGGCCGAAG TTGCTGAAAA CGTGGTGGAG ACCTTCGAGG AGCCAGCGGC ACCTATGGAA 
GCCGAGGTGG CCGAGACGAT CCTGGAGACA AATGTGGTGT CCACCACTGA GCTGCCGGAG 
ATCAAGCTGT TCGGCCGCTG GTCTTGCGAC GATGTCACCG TTAACGACAT CTCTCTGCAG 
GATTACATCT CGGTGAAGGA GAAGTTTGCC CGCTATCTTC CCCATTCCGC CGGACGTTAT 
GCCGCCAAGC GTTTCCGCAA GGCCCAGTGC CCCATTGTGG AGCGTTTGAC CTGCTCCCTG 
ATGATGAAGG GTCGCAACAA CGGCAAGAAG CTGATGGCCT GCCGCATCGT CAAGCACTCG 
TTCGAGATCA TTCATCTGCT CACCGGGGAG AACCCTCTGC AGATCCTGGT CAGCGCCATC 
ATCAACTCGG GACCCCGTGA GGACTCCACC CGTATTGGAC GTGCCGGTAC CGTCCGTCGC 
CAGGCCGTCG ATGTGTCGCC CCTGCGTCGC GTCAACCAGG CTATCTGGCT GCTGTGCACT 
GGAGCTCGTG AGGCTGCCTT CAGGAACATC AAGACCATCG CCGAGTGCCT GGCTGATGAG 
CTGATCAACG CTGCTAAGGG ATCTTCCAAC TCGTACGCCA TCAAGAAGAA GGATGAGTTG 
GAGCGTGTCG CCAAGTCCAA CCGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RpS5a ( NM_001298432.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285361.1 Drosophila melanogaster ribosomal protein S5a, transcript variant C (RpS5a), mRNA.

Price & Availability↑

CloneID	OFa01874
Clone ID Related Accession (Same CDS sequence)	NM_001298431.1 , NM_001298432.1 , NM_078658.3 , NM_001298433.1
Accession Version	NM_001298432.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ribosomal protein S5a, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGAAG TTGCTGAAAA CGTGGTGGAG ACCTTCGAGG AGCCAGCGGC ACCTATGGAA 
GCCGAGGTGG CCGAGACGAT CCTGGAGACA AATGTGGTGT CCACCACTGA GCTGCCGGAG 
ATCAAGCTGT TCGGCCGCTG GTCTTGCGAC GATGTCACCG TTAACGACAT CTCTCTGCAG 
GATTACATCT CGGTGAAGGA GAAGTTTGCC CGCTATCTTC CCCATTCCGC CGGACGTTAT 
GCCGCCAAGC GTTTCCGCAA GGCCCAGTGC CCCATTGTGG AGCGTTTGAC CTGCTCCCTG 
ATGATGAAGG GTCGCAACAA CGGCAAGAAG CTGATGGCCT GCCGCATCGT CAAGCACTCG 
TTCGAGATCA TTCATCTGCT CACCGGGGAG AACCCTCTGC AGATCCTGGT CAGCGCCATC 
ATCAACTCGG GACCCCGTGA GGACTCCACC CGTATTGGAC GTGCCGGTAC CGTCCGTCGC 
CAGGCCGTCG ATGTGTCGCC CCTGCGTCGC GTCAACCAGG CTATCTGGCT GCTGTGCACT 
GGAGCTCGTG AGGCTGCCTT CAGGAACATC AAGACCATCG CCGAGTGCCT GGCTGATGAG 
CTGATCAACG CTGCTAAGGG ATCTTCCAAC TCGTACGCCA TCAAGAAGAA GGATGAGTTG 
GAGCGTGTCG CCAAGTCCAA CCGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285361.1
CDS	579..1265
Translation	MAEVAENVVETFEEPAAPMEAEVAETILETNVVSTTELPEIKLFGRWSCDDVTVNDISLQDYISVKEKFARYLPHSAGRYAAKRFRKAQCPIVERLTCSLMMKGRNNGKKLMACRIVKHSFEIIHLLTGENPLQILVSAIINSGPREDSTRIGRAGTVRRQAVDVSPLRRVNQAIWLLCTGAREAAFRNIKTIAECLADELINAAKGSSNSYAIKKKDELERVAKSNR

Target ORF information:

RefSeq Version	NM_001298432.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ribosomal protein S5a, transcript variant C (RpS5a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298432.1

ORF Insert Sequence:

ATGGCCGAAG TTGCTGAAAA CGTGGTGGAG ACCTTCGAGG AGCCAGCGGC ACCTATGGAA 
GCCGAGGTGG CCGAGACGAT CCTGGAGACA AATGTGGTGT CCACCACTGA GCTGCCGGAG 
ATCAAGCTGT TCGGCCGCTG GTCTTGCGAC GATGTCACCG TTAACGACAT CTCTCTGCAG 
GATTACATCT CGGTGAAGGA GAAGTTTGCC CGCTATCTTC CCCATTCCGC CGGACGTTAT 
GCCGCCAAGC GTTTCCGCAA GGCCCAGTGC CCCATTGTGG AGCGTTTGAC CTGCTCCCTG 
ATGATGAAGG GTCGCAACAA CGGCAAGAAG CTGATGGCCT GCCGCATCGT CAAGCACTCG 
TTCGAGATCA TTCATCTGCT CACCGGGGAG AACCCTCTGC AGATCCTGGT CAGCGCCATC 
ATCAACTCGG GACCCCGTGA GGACTCCACC CGTATTGGAC GTGCCGGTAC CGTCCGTCGC 
CAGGCCGTCG ATGTGTCGCC CCTGCGTCGC GTCAACCAGG CTATCTGGCT GCTGTGCACT 
GGAGCTCGTG AGGCTGCCTT CAGGAACATC AAGACCATCG CCGAGTGCCT GGCTGATGAG 
CTGATCAACG CTGCTAAGGG ATCTTCCAAC TCGTACGCCA TCAAGAAGAA GGATGAGTTG 
GAGCGTGTCG CCAAGTCCAA CCGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RpS5a ( NM_078658.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523382.1 Drosophila melanogaster ribosomal protein S5a, transcript variant A (RpS5a), mRNA.

Price & Availability↑

CloneID	OFa01874
Clone ID Related Accession (Same CDS sequence)	NM_001298431.1 , NM_001298432.1 , NM_078658.3 , NM_001298433.1
Accession Version	NM_078658.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ribosomal protein S5a, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_078658.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGAAG TTGCTGAAAA CGTGGTGGAG ACCTTCGAGG AGCCAGCGGC ACCTATGGAA 
GCCGAGGTGG CCGAGACGAT CCTGGAGACA AATGTGGTGT CCACCACTGA GCTGCCGGAG 
ATCAAGCTGT TCGGCCGCTG GTCTTGCGAC GATGTCACCG TTAACGACAT CTCTCTGCAG 
GATTACATCT CGGTGAAGGA GAAGTTTGCC CGCTATCTTC CCCATTCCGC CGGACGTTAT 
GCCGCCAAGC GTTTCCGCAA GGCCCAGTGC CCCATTGTGG AGCGTTTGAC CTGCTCCCTG 
ATGATGAAGG GTCGCAACAA CGGCAAGAAG CTGATGGCCT GCCGCATCGT CAAGCACTCG 
TTCGAGATCA TTCATCTGCT CACCGGGGAG AACCCTCTGC AGATCCTGGT CAGCGCCATC 
ATCAACTCGG GACCCCGTGA GGACTCCACC CGTATTGGAC GTGCCGGTAC CGTCCGTCGC 
CAGGCCGTCG ATGTGTCGCC CCTGCGTCGC GTCAACCAGG CTATCTGGCT GCTGTGCACT 
GGAGCTCGTG AGGCTGCCTT CAGGAACATC AAGACCATCG CCGAGTGCCT GGCTGATGAG 
CTGATCAACG CTGCTAAGGG ATCTTCCAAC TCGTACGCCA TCAAGAAGAA GGATGAGTTG 
GAGCGTGTCG CCAAGTCCAA CCGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523382.1
CDS	63..749
Translation	MAEVAENVVETFEEPAAPMEAEVAETILETNVVSTTELPEIKLFGRWSCDDVTVNDISLQDYISVKEKFARYLPHSAGRYAAKRFRKAQCPIVERLTCSLMMKGRNNGKKLMACRIVKHSFEIIHLLTGENPLQILVSAIINSGPREDSTRIGRAGTVRRQAVDVSPLRRVNQAIWLLCTGAREAAFRNIKTIAECLADELINAAKGSSNSYAIKKKDELERVAKSNR

Target ORF information:

RefSeq Version	NM_078658.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ribosomal protein S5a, transcript variant A (RpS5a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078658.3

ORF Insert Sequence:

ATGGCCGAAG TTGCTGAAAA CGTGGTGGAG ACCTTCGAGG AGCCAGCGGC ACCTATGGAA 
GCCGAGGTGG CCGAGACGAT CCTGGAGACA AATGTGGTGT CCACCACTGA GCTGCCGGAG 
ATCAAGCTGT TCGGCCGCTG GTCTTGCGAC GATGTCACCG TTAACGACAT CTCTCTGCAG 
GATTACATCT CGGTGAAGGA GAAGTTTGCC CGCTATCTTC CCCATTCCGC CGGACGTTAT 
GCCGCCAAGC GTTTCCGCAA GGCCCAGTGC CCCATTGTGG AGCGTTTGAC CTGCTCCCTG 
ATGATGAAGG GTCGCAACAA CGGCAAGAAG CTGATGGCCT GCCGCATCGT CAAGCACTCG 
TTCGAGATCA TTCATCTGCT CACCGGGGAG AACCCTCTGC AGATCCTGGT CAGCGCCATC 
ATCAACTCGG GACCCCGTGA GGACTCCACC CGTATTGGAC GTGCCGGTAC CGTCCGTCGC 
CAGGCCGTCG ATGTGTCGCC CCTGCGTCGC GTCAACCAGG CTATCTGGCT GCTGTGCACT 
GGAGCTCGTG AGGCTGCCTT CAGGAACATC AAGACCATCG CCGAGTGCCT GGCTGATGAG 
CTGATCAACG CTGCTAAGGG ATCTTCCAAC TCGTACGCCA TCAAGAAGAA GGATGAGTTG 
GAGCGTGTCG CCAAGTCCAA CCGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RpS5a ( NM_001298433.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285362.1 Drosophila melanogaster ribosomal protein S5a, transcript variant D (RpS5a), mRNA.

Price & Availability↑

CloneID	OFa01874
Clone ID Related Accession (Same CDS sequence)	NM_001298431.1 , NM_001298432.1 , NM_078658.3 , NM_001298433.1
Accession Version	NM_001298433.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ribosomal protein S5a, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGAAG TTGCTGAAAA CGTGGTGGAG ACCTTCGAGG AGCCAGCGGC ACCTATGGAA 
GCCGAGGTGG CCGAGACGAT CCTGGAGACA AATGTGGTGT CCACCACTGA GCTGCCGGAG 
ATCAAGCTGT TCGGCCGCTG GTCTTGCGAC GATGTCACCG TTAACGACAT CTCTCTGCAG 
GATTACATCT CGGTGAAGGA GAAGTTTGCC CGCTATCTTC CCCATTCCGC CGGACGTTAT 
GCCGCCAAGC GTTTCCGCAA GGCCCAGTGC CCCATTGTGG AGCGTTTGAC CTGCTCCCTG 
ATGATGAAGG GTCGCAACAA CGGCAAGAAG CTGATGGCCT GCCGCATCGT CAAGCACTCG 
TTCGAGATCA TTCATCTGCT CACCGGGGAG AACCCTCTGC AGATCCTGGT CAGCGCCATC 
ATCAACTCGG GACCCCGTGA GGACTCCACC CGTATTGGAC GTGCCGGTAC CGTCCGTCGC 
CAGGCCGTCG ATGTGTCGCC CCTGCGTCGC GTCAACCAGG CTATCTGGCT GCTGTGCACT 
GGAGCTCGTG AGGCTGCCTT CAGGAACATC AAGACCATCG CCGAGTGCCT GGCTGATGAG 
CTGATCAACG CTGCTAAGGG ATCTTCCAAC TCGTACGCCA TCAAGAAGAA GGATGAGTTG 
GAGCGTGTCG CCAAGTCCAA CCGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285362.1
CDS	8..694
Translation	MAEVAENVVETFEEPAAPMEAEVAETILETNVVSTTELPEIKLFGRWSCDDVTVNDISLQDYISVKEKFARYLPHSAGRYAAKRFRKAQCPIVERLTCSLMMKGRNNGKKLMACRIVKHSFEIIHLLTGENPLQILVSAIINSGPREDSTRIGRAGTVRRQAVDVSPLRRVNQAIWLLCTGAREAAFRNIKTIAECLADELINAAKGSSNSYAIKKKDELERVAKSNR

Target ORF information:

RefSeq Version	NM_001298433.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ribosomal protein S5a, transcript variant D (RpS5a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298433.1

ORF Insert Sequence:

ATGGCCGAAG TTGCTGAAAA CGTGGTGGAG ACCTTCGAGG AGCCAGCGGC ACCTATGGAA 
GCCGAGGTGG CCGAGACGAT CCTGGAGACA AATGTGGTGT CCACCACTGA GCTGCCGGAG 
ATCAAGCTGT TCGGCCGCTG GTCTTGCGAC GATGTCACCG TTAACGACAT CTCTCTGCAG 
GATTACATCT CGGTGAAGGA GAAGTTTGCC CGCTATCTTC CCCATTCCGC CGGACGTTAT 
GCCGCCAAGC GTTTCCGCAA GGCCCAGTGC CCCATTGTGG AGCGTTTGAC CTGCTCCCTG 
ATGATGAAGG GTCGCAACAA CGGCAAGAAG CTGATGGCCT GCCGCATCGT CAAGCACTCG 
TTCGAGATCA TTCATCTGCT CACCGGGGAG AACCCTCTGC AGATCCTGGT CAGCGCCATC 
ATCAACTCGG GACCCCGTGA GGACTCCACC CGTATTGGAC GTGCCGGTAC CGTCCGTCGC 
CAGGCCGTCG ATGTGTCGCC CCTGCGTCGC GTCAACCAGG CTATCTGGCT GCTGTGCACT 
GGAGCTCGTG AGGCTGCCTT CAGGAACATC AAGACCATCG CCGAGTGCCT GGCTGATGAG 
CTGATCAACG CTGCTAAGGG ATCTTCCAAC TCGTACGCCA TCAAGAAGAA GGATGAGTTG 
GAGCGTGTCG CCAAGTCCAA CCGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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RpS5a cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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