if cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	if
Entrez Gene ID	32661
Full Name	inflated
Synonyms	CG9623,CT27194,Dmel\CG9623,If,ItgaPS2,PS 2,PS2,PS2(c),PS2(m8),PS2(m8)alpha,PS2alpha,PSalpha2,aPS2,alpha-PS2,alpha2Int,alphaPS2,alphaPS2 integrin,alphaPS2C,alphaPS2m8,alpha[[PS2(ms8)]],alpha[[PS2]],in,inf
General protein information	Preferred Names inflated Names CG9623 gene product from transcript CG9623-RC CG9623-PB CG9623-PC CG9623-PD CG9623-PF PS II alpha alpha integrin alpha-PS2-integrin alpha-integrin alpha2 integrin alphaPS2 alphaPS2 integrin alphaPS2-integrin alpha[[PS2]] integrin if-PB if-PC if-PD if-PF integrin PSI alpha subunit integrin alphaPS2 subunit position-specific antigen 2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 15A5-15A7\|1-55 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001169307.1	NP_001162778.1	inflated, isoform F
NM_078654.2	NP_523378.2	inflated, isoform B
NM_167544.2	NP_728021.2	inflated, isoform C
NM_001169306.2	NP_001162777.1	inflated, isoform D

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	ITGA8	NP_990619.1
Caenorhabditis elegans (roundworm)	pat-2	NP_498948.1
Pan troglodytes (chimpanzee)	ITGA8	XP_507671.2
Bos taurus (cattle)	ITGA8	XP_002692127.1
	ITGA8	XP_001089317.1
Drosophila melanogaster (fruit fly)	if	NP_001162778.1
Canis lupus familiaris (dog)	ITGA8	XP_005617255.1
Mus musculus (house mouse)	Itga8	NP_001001309.1
Homo sapiens (human)	ITGA8	NP_003629.1
Rattus norvegicus (Norway rat)	Itga8	NP_001167443.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000032	XP_311131.5
Xenopus tropicalis (tropical clawed frog)	itga8	XP_002939074.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the conserved transmembrane Drosophila proteoglycan Kon-tiki (Kon, also known as Perdido) interacts with the alphaPS2betaPS integrin (alphaPS2 is encoded by inflated and betaPS by myospheroid) to mediate muscle-tendon adhesion.
Title: Kon-tiki enhances PS2 integrin adhesion and localizes its ligand, Thrombospondin, in the myotendinous junction.

the integrin heterodimer alphaPS3/betaPS becomes apically enriched and is required for engulfment by the epithelial follicle cells of the Drosophila ovary.
Title: Polarization of the epithelial layer and apical localization of integrins are required for engulfment of apoptotic cells in the Drosophila ovary.

Study presents evidence that alphaPS2 and alphaPS3 integrin have different and specific functions in the development of retinal glia
Title: Drosophila PS2 and PS3 integrins play distinct roles in retinal photoreceptors-glia interactions.

Data indicate that Tsp66E and Tsp74F mutant ovaries had elevated inflated gene product alphaPS2 integrin levels and reduced endocytic trafficking.
Title: Tsp66E, the Drosophila KAI1 homologue, and Tsp74F function to regulate ovarian follicle cell and wing development by stabilizing integrin localization.

These data suggest a model in which ERK phosphorylation must be spatially coupled to integrin-mediated DIM-7 activation to make a complex that can be imported efficiently.
Title: Nuclear localization of the ERK MAP kinase mediated by Drosophila alphaPS2betaPS integrin and importin-7.

The following if gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the if cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01838	NM_078654.2 Latest version!	Drosophila melanogaster inflated (if), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OFa01838	NM_001169306.2 Latest version!	Drosophila melanogaster inflated (if), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OFa01838	NM_001169307.1 Latest version!	Drosophila melanogaster inflated (if), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OFa03066	NM_167544.2 Latest version!	Drosophila melanogaster inflated (if), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

if ( NM_001169307.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001162778.1 Drosophila melanogaster inflated (if), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa01838
Clone ID Related Accession (Same CDS sequence)	NM_001169307.1 , NM_078654.2 , NM_001169306.2
Accession Version	NM_001169307.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4191bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	inflated, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTGGAG ATTCCATCCA CCGAAGAAGA ATGGCTCTTC ACTGCCCCAT AACGAGCCTG 
ATTCTGCTCC TGATCGCCAT GTCCGCCCAT GGTTACAACA TCGACCTGCC CAGCTACGTC 
CGTTTCCGGC AGTCCAGCAA CTCGATGTTC GGATTCAGCA TTGCGATGCA CAAGGGTCGC 
AGTGGATTCT ACGGCAATCA GAATAATGTC AGTTTGATTG TCGGAGCACC CAAATTCGAC 
ACCTCCCGCT ATCAACAGGG CGTGACCGAG GCAGGAGGCG TTTTCAAATG CAGCCTCAAC 
GACGACGACT GCAAATTGGT GCCATTCGAC TCCAAAGGCA ACAATCGCAA TGTGGACAAG 
GAGGTCGTGG ACAGGAAATC CTATCAATGG TTGGGCGCCA CTGTGGCAAC CGGTCGGGAT 
AGCGACTTGG TTGTGGCCTG TGCACCGCGC TATGTCTTCC ACACGATGAC GCCATCGAGG 
GCGTTCCGCA TTGATCCCGT GGGCACCTGC TTCACCTCGC ACAACTTCGA GGAGTTCTAC 
GAGGTCTCCC CCTGCCGGAC AAACAACTGG GGTTATCATC GCCAGGGCTC GTGTCAGGCT 
GGATTCAGTG CAGCGATCAA CGGGAATGGC TCGCGTCTGT TCATTGGCGC ACCTGGCAGT 
TGGTATTGGC AAGGACAAAC CTACTCGATA CCGCCCGATG CCAAGTTTCC ATTTAAGCCG 
CCTCTTTATC AGCCCTTCGG CACTGGAGGT ATGGCCAGCT CCCACGATGT GACCAGGCCG 
GAGAACCAAG TGTTCTCCAC TTCGGAGAGT GCCTCGGTGA ACGATGACTC CTATTTGGGC 
TACTCGATGG TCACCGGTGA TTTCGATGGC GATCGCAGCG AGGATGTGGC CATTGGAATG 
CCCCGCGGTG GTAATCTCGT GGGAAGGATC GTCGTTAATC GCTGGAACAT GGCGAACATC 
TTTAATATCA CGGGTCGCCA GATTGGCGAA TACTTTGGCT ATTCCTTGGC CACCAGCGAT 
GTGGATGGCG ACGGCCTGGA CGATCTGCTT ATCGGTGCTC CCATGTACAC GGATCCCGAC 
AACGTGGAGG GCAAATACGA TGTGGGCAGG GTGTACATCC TGCTGCAGGG CGGTCCCACC 
GAGGAGAAGC GTTGGACGAC GGAGCACATT CGCGATGGCT ACCACAGCAA GGGTAGATTC 
GGCTTGGCCC TGACCACCTT GGGCGATGTG AATGGCGATG GCTATGGAGA CTTCGCCGTT 
GGTGCCCCCT ACGATGGACC CGAGGGTCGT GGAGTGGTCT ATATCTTCCA TGGCTCGCCG 
ATGGGTCCAC TGGCCAAACC GTCGCAGATC ATTAAGTCCG AGCAGTTGGT GGAGGGAGCT 
CCCTATCCGC GAACATTTGG CTTCGCCTTG TCCGGCGGTC TGGACATGGA CGGCAATACC 
TATCCAGATC TCGCCGTGGG CGCCTACAGC TCCGACCAGG TGTTCATCTT TAAGTCGCGA 
CCTGTGGCCG CTGTTAATGC CGAAACCAGT TTCGCCAGCA ACTCGAAGCT GATCAGTCTG 
GACGACAGGA GCTGTCAGCT GGTCAGGGAT CACAAGAAGG TGCCATGCAT GCTACTGACC 
ACCTGCTGGA GCTACACGGG TCGCTATCTG CCCGAGCAAC TGGACTTCGA TGTGTCCTGG 
CTTCTGGATG CCAAAAAGTT GCTCAATCCT CGCATGTTCT TCCTGAGGGA TGAGGGCAAG 
AACATCAGGA ATCAGACGAT CCGCTTGAAC TATGGCCAGA AGTATTGCCT GAACGAGACC 
GTCTATCTGC TGGACAAGGT GCAGGATAAG TTGACGCCTC TGGAAGTGGA AGCCCGCTAC 
AATCTGCGCA GTAGTCGTCC CTTGGACCCG ATGGTTCGTC ATAGGAGGAG CATCCTTGAG 
CCGGTTATTG ATCAGAACCG CGAGATCGTC CTGCGGGATG CCATCAACAT TCAGAAGAAC 
TGTGGGCCCG ACAATATTTG CGAGCCAGAT CTCAAGCTGA AAGTCAGCAC TGTGGACAAG 
TATCTGTTTG GCTCACCCGA ACCTCTGGTC ATCGAGGTCT TTATCTCGAA CACCAATGAG 
GATGCCTTCG AGGCTGCCTT CTACATGGTC ACCCCGCCGG ATCTGCAGTT CAGGAAGCTG 
CAGCAGCTTG GCGAGAAGAA GGACACGCCC ATCACGTGCT CGCCGCCCAC TCCGGAGAAC 
AACCACACAC TCAAGTGCGA TATTGGAAAC CCGTTAGAGT CTGGCAAGAT TGCACACTTC 
AAGATCAGTT TGGTGCCGGA GGAGAAGTAT GGTAGCTCGT CCAGCTACGA CTTCTACTGG 
GAGGCAAATT CGACGAACTT GGAGAAACCC GGCTCCGAAT ACGACAACAA GATCCGCCAA 
AGCGTGGGTA TCTGGGTGGA CACGGATCTG GACATCAAGG GCACCTCCCT GCCAGACTAC 
CAGCTGTACA AGGCCGACGA CTACAAAGAG TTGGAGAATG CCACCAAGGA GGACGATATT 
GGACCTCAGG TGGTGCACAT CTACGAGATC CGCAACAATC GTCCCTCAAT CATCGAGGAG 
GCGGAGGTCT TCATCCATCT GCCCTACGAG ACAATCGTCG GGGATCCGCT AATGTACCTG 
TTGAATCAAC CAGAAACCGG GGGCAAGATC CAGTGCGACG ATGTGGCATT CAATGAGTAC 
AATCTGCTGC TGGACGAGAA GCTGGTAAAG AAGTCCTATC TGCAGGCCCA GGGCGCCATT 
TGGAATTCGG CCCAGGTCTC TGGCCAATCG TCTAGCTCCT CGTCATCCGG CGGTGCCAGC 
GTTCACATCG AGAAGGCTCG CGGTGAAGGT TTCGTTCGAG GAGTCTTGGT CAGCAATAGC 
ACCGATGCGG GTGACAAATT AAGTCCCAAA CAAGTGGAGC AACGGCGGCA GGAGGACACA 
TTGGAGGCGC TTGGAGATGC ATCCTTCGTG CACCGCGATC GCGCCAGTCA GGCGGTCCAG 
GAACCACAAG TTAATCAGAC CAGCTTCACT ACCTACTCCA CCAGCAGCTC TTCCTCCGGT 
TCGGGTGCTC CATCGGCCCA ACTGCGTGGA CACAGCACAC AGGGTCACAT CCAGATGGCC 
GGACCAGTTC AGCACACGAG CAGCTCCTCC TCTTCCAACT ACCGATCTTG GCCAGCGCAG 
CAGCAGCAGC AGCATCAGCA ACTACTTTTA GCCGGTTCCG GAGGATCTGG CCTTGGATCT 
CCGGTTACCT TTAACGACAA ATCGCAATTT GGTGGCCGTA ACAACAATTT CCATACCGGT 
ACTCTCGATT TGGGCACCCT AAACCGAGGC AATGTGGACA ACGAGCTGTA CAGGAGTCAG 
GGTCAATATC AGAATCCCAG CCAGAGTCTG GGCCAAAGCC AGGGTCAGTT CCAGGCCAAT 
GCCAACCAGG GACATTACCA GGGACAAAAT CAGGCCCAGT TCCAGGCACG TAATCCGGGC 
TTCCAGGGAC AGACTTCCTA CCAGGGACAG ACGCAGTACA GTGGCCAACC AGGTGGCTAT 
CAGACCCACC ATGTTACCTA CTCCTCTGGC AGCAAGCCCT ACTATGGCCG CGAGAATGAG 
GACTTCTACG ACGAGGATAA CCTGCAGCAG GCAACGCCTG GACACTGGAG CAGCAGCAGT 
AGCTCCAGTT CCAGTTCCGG CACACGCCGC CTGCGGAGGT CCAACGATAA GGATGGGGCA 
ACGGAGAAGC CGCTACAGAT CGACCTGAAC TCACCATGCC AGTCGGCCAG GTGTAAGAGC 
ATCCGCTGCG TGGTCACAAA TCTAGGCACC GAGGACGGCG ACGCCGCCTT TGTGGCCATC 
CGTGCACGCA TGGTGGCCAA GACGATGGAG AAGCTGGCCT CCAACGTGCC ACTCAATGTG 
TCCACCCTGG CGGTGGCCAA TGTCACACTG CTGCCCTTCA TTGGAGCGCC GAAGGATGCC 
ATTGTGAAGA CGCACGAGAT CTTCTACAAG GCGGAACCGG AACCGCTTCA GGTGCCCGAT 
GTGGTGCCTC TGTGGGTCGT CGTACTGGCC GCCTGTGCCG GAGCCCTCAT CTTCCTGCTG 
CTCGTCTGGC TGCTCTACAA GTGCGGCTTC TTTAACCGCA ACCGGCCAAC GGATCACTCG 
CAGGAGCGGC AGCCACTGAG GAATGGATAC CACGGCGACG AGCACCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162778.1
CDS	352..4542
Translation	MSGDSIHRRRMALHCPITSLILLLIAMSAHGYNIDLPSYVRFRQSSNSMFGFSIAMHKGRSGFYGNQNNVSLIVGAPKFDTSRYQQGVTEAGGVFKCSLNDDDCKLVPFDSKGNNRNVDKEVVDRKSYQWLGATVATGRDSDLVVACAPRYVFHTMTPSRAFRIDPVGTCFTSHNFEEFYEVSPCRTNNWGYHRQGSCQAGFSAAINGNGSRLFIGAPGSWYWQGQTYSIPPDAKFPFKPPLYQPFGTGGMASSHDVTRPENQVFSTSESASVNDDSYLGYSMVTGDFDGDRSEDVAIGMPRGGNLVGRIVVNRWNMANIFNITGRQIGEYFGYSLATSDVDGDGLDDLLIGAPMYTDPDNVEGKYDVGRVYILLQGGPTEEKRWTTEHIRDGYHSKGRFGLALTTLGDVNGDGYGDFAVGAPYDGPEGRGVVYIFHGSPMGPLAKPSQIIKSEQLVEGAPYPRTFGFALSGGLDMDGNTYPDLAVGAYSSDQVFIFKSRPVAAVNAETSFASNSKLISLDDRSCQLVRDHKKVPCMLLTTCWSYTGRYLPEQLDFDVSWLLDAKKLLNPRMFFLRDEGKNIRNQTIRLNYGQKYCLNETVYLLDKVQDKLTPLEVEARYNLRSSRPLDPMVRHRRSILEPVIDQNREIVLRDAINIQKNCGPDNICEPDLKLKVSTVDKYLFGSPEPLVIEVFISNTNEDAFEAAFYMVTPPDLQFRKLQQLGEKKDTPITCSPPTPENNHTLKCDIGNPLESGKIAHFKISLVPEEKYGSSSSYDFYWEANSTNLEKPGSEYDNKIRQSVGIWVDTDLDIKGTSLPDYQLYKADDYKELENATKEDDIGPQVVHIYEIRNNRPSIIEEAEVFIHLPYETIVGDPLMYLLNQPETGGKIQCDDVAFNEYNLLLDEKLVKKSYLQAQGAIWNSAQVSGQSSSSSSSGGASVHIEKARGEGFVRGVLVSNSTDAGDKLSPKQVEQRRQEDTLEALGDASFVHRDRASQAVQEPQVNQTSFTTYSTSSSSSGSGAPSAQLRGHSTQGHIQMAGPVQHTSSSSSSNYRSWPAQQQQQHQQLLLAGSGGSGLGSPVTFNDKSQFGGRNNNFHTGTLDLGTLNRGNVDNELYRSQGQYQNPSQSLGQSQGQFQANANQGHYQGQNQAQFQARNPGFQGQTSYQGQTQYSGQPGGYQTHHVTYSSGSKPYYGRENEDFYDEDNLQQATPGHWSSSSSSSSSSGTRRLRRSNDKDGATEKPLQIDLNSPCQSARCKSIRCVVTNLGTEDGDAAFVAIRARMVAKTMEKLASNVPLNVSTLAVANVTLLPFIGAPKDAIVKTHEIFYKAEPEPLQVPDVVPLWVVVLAACAGALIFLLLVWLLYKCGFFNRNRPTDHSQERQPLRNGYHGDEHL

Target ORF information:

RefSeq Version	NM_001169307.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster inflated (if), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169307.1

ORF Insert Sequence:

ATGAGTGGAG ATTCCATCCA CCGAAGAAGA ATGGCTCTTC ACTGCCCCAT AACGAGCCTG 
ATTCTGCTCC TGATCGCCAT GTCCGCCCAT GGTTACAACA TCGACCTGCC CAGCTACGTC 
CGTTTCCGGC AGTCCAGCAA CTCGATGTTC GGATTCAGCA TTGCGATGCA CAAGGGTCGC 
AGTGGATTCT ACGGCAATCA GAATAATGTC AGTTTGATTG TCGGAGCACC CAAATTCGAC 
ACCTCCCGCT ATCAACAGGG CGTGACCGAG GCAGGAGGCG TTTTCAAATG CAGCCTCAAC 
GACGACGACT GCAAATTGGT GCCATTCGAC TCCAAAGGCA ACAATCGCAA TGTGGACAAG 
GAGGTCGTGG ACAGGAAATC CTATCAATGG TTGGGCGCCA CTGTGGCAAC CGGTCGGGAT 
AGCGACTTGG TTGTGGCCTG TGCACCGCGC TATGTCTTCC ACACGATGAC GCCATCGAGG 
GCGTTCCGCA TTGATCCCGT GGGCACCTGC TTCACCTCGC ACAACTTCGA GGAGTTCTAC 
GAGGTCTCCC CCTGCCGGAC AAACAACTGG GGTTATCATC GCCAGGGCTC GTGTCAGGCT 
GGATTCAGTG CAGCGATCAA CGGGAATGGC TCGCGTCTGT TCATTGGCGC ACCTGGCAGT 
TGGTATTGGC AAGGACAAAC CTACTCGATA CCGCCCGATG CCAAGTTTCC ATTTAAGCCG 
CCTCTTTATC AGCCCTTCGG CACTGGAGGT ATGGCCAGCT CCCACGATGT GACCAGGCCG 
GAGAACCAAG TGTTCTCCAC TTCGGAGAGT GCCTCGGTGA ACGATGACTC CTATTTGGGC 
TACTCGATGG TCACCGGTGA TTTCGATGGC GATCGCAGCG AGGATGTGGC CATTGGAATG 
CCCCGCGGTG GTAATCTCGT GGGAAGGATC GTCGTTAATC GCTGGAACAT GGCGAACATC 
TTTAATATCA CGGGTCGCCA GATTGGCGAA TACTTTGGCT ATTCCTTGGC CACCAGCGAT 
GTGGATGGCG ACGGCCTGGA CGATCTGCTT ATCGGTGCTC CCATGTACAC GGATCCCGAC 
AACGTGGAGG GCAAATACGA TGTGGGCAGG GTGTACATCC TGCTGCAGGG CGGTCCCACC 
GAGGAGAAGC GTTGGACGAC GGAGCACATT CGCGATGGCT ACCACAGCAA GGGTAGATTC 
GGCTTGGCCC TGACCACCTT GGGCGATGTG AATGGCGATG GCTATGGAGA CTTCGCCGTT 
GGTGCCCCCT ACGATGGACC CGAGGGTCGT GGAGTGGTCT ATATCTTCCA TGGCTCGCCG 
ATGGGTCCAC TGGCCAAACC GTCGCAGATC ATTAAGTCCG AGCAGTTGGT GGAGGGAGCT 
CCCTATCCGC GAACATTTGG CTTCGCCTTG TCCGGCGGTC TGGACATGGA CGGCAATACC 
TATCCAGATC TCGCCGTGGG CGCCTACAGC TCCGACCAGG TGTTCATCTT TAAGTCGCGA 
CCTGTGGCCG CTGTTAATGC CGAAACCAGT TTCGCCAGCA ACTCGAAGCT GATCAGTCTG 
GACGACAGGA GCTGTCAGCT GGTCAGGGAT CACAAGAAGG TGCCATGCAT GCTACTGACC 
ACCTGCTGGA GCTACACGGG TCGCTATCTG CCCGAGCAAC TGGACTTCGA TGTGTCCTGG 
CTTCTGGATG CCAAAAAGTT GCTCAATCCT CGCATGTTCT TCCTGAGGGA TGAGGGCAAG 
AACATCAGGA ATCAGACGAT CCGCTTGAAC TATGGCCAGA AGTATTGCCT GAACGAGACC 
GTCTATCTGC TGGACAAGGT GCAGGATAAG TTGACGCCTC TGGAAGTGGA AGCCCGCTAC 
AATCTGCGCA GTAGTCGTCC CTTGGACCCG ATGGTTCGTC ATAGGAGGAG CATCCTTGAG 
CCGGTTATTG ATCAGAACCG CGAGATCGTC CTGCGGGATG CCATCAACAT TCAGAAGAAC 
TGTGGGCCCG ACAATATTTG CGAGCCAGAT CTCAAGCTGA AAGTCAGCAC TGTGGACAAG 
TATCTGTTTG GCTCACCCGA ACCTCTGGTC ATCGAGGTCT TTATCTCGAA CACCAATGAG 
GATGCCTTCG AGGCTGCCTT CTACATGGTC ACCCCGCCGG ATCTGCAGTT CAGGAAGCTG 
CAGCAGCTTG GCGAGAAGAA GGACACGCCC ATCACGTGCT CGCCGCCCAC TCCGGAGAAC 
AACCACACAC TCAAGTGCGA TATTGGAAAC CCGTTAGAGT CTGGCAAGAT TGCACACTTC 
AAGATCAGTT TGGTGCCGGA GGAGAAGTAT GGTAGCTCGT CCAGCTACGA CTTCTACTGG 
GAGGCAAATT CGACGAACTT GGAGAAACCC GGCTCCGAAT ACGACAACAA GATCCGCCAA 
AGCGTGGGTA TCTGGGTGGA CACGGATCTG GACATCAAGG GCACCTCCCT GCCAGACTAC 
CAGCTGTACA AGGCCGACGA CTACAAAGAG TTGGAGAATG CCACCAAGGA GGACGATATT 
GGACCTCAGG TGGTGCACAT CTACGAGATC CGCAACAATC GTCCCTCAAT CATCGAGGAG 
GCGGAGGTCT TCATCCATCT GCCCTACGAG ACAATCGTCG GGGATCCGCT AATGTACCTG 
TTGAATCAAC CAGAAACCGG GGGCAAGATC CAGTGCGACG ATGTGGCATT CAATGAGTAC 
AATCTGCTGC TGGACGAGAA GCTGGTAAAG AAGTCCTATC TGCAGGCCCA GGGCGCCATT 
TGGAATTCGG CCCAGGTCTC TGGCCAATCG TCTAGCTCCT CGTCATCCGG CGGTGCCAGC 
GTTCACATCG AGAAGGCTCG CGGTGAAGGT TTCGTTCGAG GAGTCTTGGT CAGCAATAGC 
ACCGATGCGG GTGACAAATT AAGTCCCAAA CAAGTGGAGC AACGGCGGCA GGAGGACACA 
TTGGAGGCGC TTGGAGATGC ATCCTTCGTG CACCGCGATC GCGCCAGTCA GGCGGTCCAG 
GAACCACAAG TTAATCAGAC CAGCTTCACT ACCTACTCCA CCAGCAGCTC TTCCTCCGGT 
TCGGGTGCTC CATCGGCCCA ACTGCGTGGA CACAGCACAC AGGGTCACAT CCAGATGGCC 
GGACCAGTTC AGCACACGAG CAGCTCCTCC TCTTCCAACT ACCGATCTTG GCCAGCGCAG 
CAGCAGCAGC AGCATCAGCA ACTACTTTTA GCCGGTTCCG GAGGATCTGG CCTTGGATCT 
CCGGTTACCT TTAACGACAA ATCGCAATTT GGTGGCCGTA ACAACAATTT CCATACCGGT 
ACTCTCGATT TGGGCACCCT AAACCGAGGC AATGTGGACA ACGAGCTGTA CAGGAGTCAG 
GGTCAATATC AGAATCCCAG CCAGAGTCTG GGCCAAAGCC AGGGTCAGTT CCAGGCCAAT 
GCCAACCAGG GACATTACCA GGGACAAAAT CAGGCCCAGT TCCAGGCACG TAATCCGGGC 
TTCCAGGGAC AGACTTCCTA CCAGGGACAG ACGCAGTACA GTGGCCAACC AGGTGGCTAT 
CAGACCCACC ATGTTACCTA CTCCTCTGGC AGCAAGCCCT ACTATGGCCG CGAGAATGAG 
GACTTCTACG ACGAGGATAA CCTGCAGCAG GCAACGCCTG GACACTGGAG CAGCAGCAGT 
AGCTCCAGTT CCAGTTCCGG CACACGCCGC CTGCGGAGGT CCAACGATAA GGATGGGGCA 
ACGGAGAAGC CGCTACAGAT CGACCTGAAC TCACCATGCC AGTCGGCCAG GTGTAAGAGC 
ATCCGCTGCG TGGTCACAAA TCTAGGCACC GAGGACGGCG ACGCCGCCTT TGTGGCCATC 
CGTGCACGCA TGGTGGCCAA GACGATGGAG AAGCTGGCCT CCAACGTGCC ACTCAATGTG 
TCCACCCTGG CGGTGGCCAA TGTCACACTG CTGCCCTTCA TTGGAGCGCC GAAGGATGCC 
ATTGTGAAGA CGCACGAGAT CTTCTACAAG GCGGAACCGG AACCGCTTCA GGTGCCCGAT 
GTGGTGCCTC TGTGGGTCGT CGTACTGGCC GCCTGTGCCG GAGCCCTCAT CTTCCTGCTG 
CTCGTCTGGC TGCTCTACAA GTGCGGCTTC TTTAACCGCA ACCGGCCAAC GGATCACTCG 
CAGGAGCGGC AGCCACTGAG GAATGGATAC CACGGCGACG AGCACCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

if ( NM_078654.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523378.2 Drosophila melanogaster inflated (if), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa01838
Clone ID Related Accession (Same CDS sequence)	NM_001169307.1 , NM_078654.2 , NM_001169306.2
Accession Version	NM_078654.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4191bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	inflated, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Nov 6, 2002 this sequence version replaced NM_078654.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTGGAG ATTCCATCCA CCGAAGAAGA ATGGCTCTTC ACTGCCCCAT AACGAGCCTG 
ATTCTGCTCC TGATCGCCAT GTCCGCCCAT GGTTACAACA TCGACCTGCC CAGCTACGTC 
CGTTTCCGGC AGTCCAGCAA CTCGATGTTC GGATTCAGCA TTGCGATGCA CAAGGGTCGC 
AGTGGATTCT ACGGCAATCA GAATAATGTC AGTTTGATTG TCGGAGCACC CAAATTCGAC 
ACCTCCCGCT ATCAACAGGG CGTGACCGAG GCAGGAGGCG TTTTCAAATG CAGCCTCAAC 
GACGACGACT GCAAATTGGT GCCATTCGAC TCCAAAGGCA ACAATCGCAA TGTGGACAAG 
GAGGTCGTGG ACAGGAAATC CTATCAATGG TTGGGCGCCA CTGTGGCAAC CGGTCGGGAT 
AGCGACTTGG TTGTGGCCTG TGCACCGCGC TATGTCTTCC ACACGATGAC GCCATCGAGG 
GCGTTCCGCA TTGATCCCGT GGGCACCTGC TTCACCTCGC ACAACTTCGA GGAGTTCTAC 
GAGGTCTCCC CCTGCCGGAC AAACAACTGG GGTTATCATC GCCAGGGCTC GTGTCAGGCT 
GGATTCAGTG CAGCGATCAA CGGGAATGGC TCGCGTCTGT TCATTGGCGC ACCTGGCAGT 
TGGTATTGGC AAGGACAAAC CTACTCGATA CCGCCCGATG CCAAGTTTCC ATTTAAGCCG 
CCTCTTTATC AGCCCTTCGG CACTGGAGGT ATGGCCAGCT CCCACGATGT GACCAGGCCG 
GAGAACCAAG TGTTCTCCAC TTCGGAGAGT GCCTCGGTGA ACGATGACTC CTATTTGGGC 
TACTCGATGG TCACCGGTGA TTTCGATGGC GATCGCAGCG AGGATGTGGC CATTGGAATG 
CCCCGCGGTG GTAATCTCGT GGGAAGGATC GTCGTTAATC GCTGGAACAT GGCGAACATC 
TTTAATATCA CGGGTCGCCA GATTGGCGAA TACTTTGGCT ATTCCTTGGC CACCAGCGAT 
GTGGATGGCG ACGGCCTGGA CGATCTGCTT ATCGGTGCTC CCATGTACAC GGATCCCGAC 
AACGTGGAGG GCAAATACGA TGTGGGCAGG GTGTACATCC TGCTGCAGGG CGGTCCCACC 
GAGGAGAAGC GTTGGACGAC GGAGCACATT CGCGATGGCT ACCACAGCAA GGGTAGATTC 
GGCTTGGCCC TGACCACCTT GGGCGATGTG AATGGCGATG GCTATGGAGA CTTCGCCGTT 
GGTGCCCCCT ACGATGGACC CGAGGGTCGT GGAGTGGTCT ATATCTTCCA TGGCTCGCCG 
ATGGGTCCAC TGGCCAAACC GTCGCAGATC ATTAAGTCCG AGCAGTTGGT GGAGGGAGCT 
CCCTATCCGC GAACATTTGG CTTCGCCTTG TCCGGCGGTC TGGACATGGA CGGCAATACC 
TATCCAGATC TCGCCGTGGG CGCCTACAGC TCCGACCAGG TGTTCATCTT TAAGTCGCGA 
CCTGTGGCCG CTGTTAATGC CGAAACCAGT TTCGCCAGCA ACTCGAAGCT GATCAGTCTG 
GACGACAGGA GCTGTCAGCT GGTCAGGGAT CACAAGAAGG TGCCATGCAT GCTACTGACC 
ACCTGCTGGA GCTACACGGG TCGCTATCTG CCCGAGCAAC TGGACTTCGA TGTGTCCTGG 
CTTCTGGATG CCAAAAAGTT GCTCAATCCT CGCATGTTCT TCCTGAGGGA TGAGGGCAAG 
AACATCAGGA ATCAGACGAT CCGCTTGAAC TATGGCCAGA AGTATTGCCT GAACGAGACC 
GTCTATCTGC TGGACAAGGT GCAGGATAAG TTGACGCCTC TGGAAGTGGA AGCCCGCTAC 
AATCTGCGCA GTAGTCGTCC CTTGGACCCG ATGGTTCGTC ATAGGAGGAG CATCCTTGAG 
CCGGTTATTG ATCAGAACCG CGAGATCGTC CTGCGGGATG CCATCAACAT TCAGAAGAAC 
TGTGGGCCCG ACAATATTTG CGAGCCAGAT CTCAAGCTGA AAGTCAGCAC TGTGGACAAG 
TATCTGTTTG GCTCACCCGA ACCTCTGGTC ATCGAGGTCT TTATCTCGAA CACCAATGAG 
GATGCCTTCG AGGCTGCCTT CTACATGGTC ACCCCGCCGG ATCTGCAGTT CAGGAAGCTG 
CAGCAGCTTG GCGAGAAGAA GGACACGCCC ATCACGTGCT CGCCGCCCAC TCCGGAGAAC 
AACCACACAC TCAAGTGCGA TATTGGAAAC CCGTTAGAGT CTGGCAAGAT TGCACACTTC 
AAGATCAGTT TGGTGCCGGA GGAGAAGTAT GGTAGCTCGT CCAGCTACGA CTTCTACTGG 
GAGGCAAATT CGACGAACTT GGAGAAACCC GGCTCCGAAT ACGACAACAA GATCCGCCAA 
AGCGTGGGTA TCTGGGTGGA CACGGATCTG GACATCAAGG GCACCTCCCT GCCAGACTAC 
CAGCTGTACA AGGCCGACGA CTACAAAGAG TTGGAGAATG CCACCAAGGA GGACGATATT 
GGACCTCAGG TGGTGCACAT CTACGAGATC CGCAACAATC GTCCCTCAAT CATCGAGGAG 
GCGGAGGTCT TCATCCATCT GCCCTACGAG ACAATCGTCG GGGATCCGCT AATGTACCTG 
TTGAATCAAC CAGAAACCGG GGGCAAGATC CAGTGCGACG ATGTGGCATT CAATGAGTAC 
AATCTGCTGC TGGACGAGAA GCTGGTAAAG AAGTCCTATC TGCAGGCCCA GGGCGCCATT 
TGGAATTCGG CCCAGGTCTC TGGCCAATCG TCTAGCTCCT CGTCATCCGG CGGTGCCAGC 
GTTCACATCG AGAAGGCTCG CGGTGAAGGT TTCGTTCGAG GAGTCTTGGT CAGCAATAGC 
ACCGATGCGG GTGACAAATT AAGTCCCAAA CAAGTGGAGC AACGGCGGCA GGAGGACACA 
TTGGAGGCGC TTGGAGATGC ATCCTTCGTG CACCGCGATC GCGCCAGTCA GGCGGTCCAG 
GAACCACAAG TTAATCAGAC CAGCTTCACT ACCTACTCCA CCAGCAGCTC TTCCTCCGGT 
TCGGGTGCTC CATCGGCCCA ACTGCGTGGA CACAGCACAC AGGGTCACAT CCAGATGGCC 
GGACCAGTTC AGCACACGAG CAGCTCCTCC TCTTCCAACT ACCGATCTTG GCCAGCGCAG 
CAGCAGCAGC AGCATCAGCA ACTACTTTTA GCCGGTTCCG GAGGATCTGG CCTTGGATCT 
CCGGTTACCT TTAACGACAA ATCGCAATTT GGTGGCCGTA ACAACAATTT CCATACCGGT 
ACTCTCGATT TGGGCACCCT AAACCGAGGC AATGTGGACA ACGAGCTGTA CAGGAGTCAG 
GGTCAATATC AGAATCCCAG CCAGAGTCTG GGCCAAAGCC AGGGTCAGTT CCAGGCCAAT 
GCCAACCAGG GACATTACCA GGGACAAAAT CAGGCCCAGT TCCAGGCACG TAATCCGGGC 
TTCCAGGGAC AGACTTCCTA CCAGGGACAG ACGCAGTACA GTGGCCAACC AGGTGGCTAT 
CAGACCCACC ATGTTACCTA CTCCTCTGGC AGCAAGCCCT ACTATGGCCG CGAGAATGAG 
GACTTCTACG ACGAGGATAA CCTGCAGCAG GCAACGCCTG GACACTGGAG CAGCAGCAGT 
AGCTCCAGTT CCAGTTCCGG CACACGCCGC CTGCGGAGGT CCAACGATAA GGATGGGGCA 
ACGGAGAAGC CGCTACAGAT CGACCTGAAC TCACCATGCC AGTCGGCCAG GTGTAAGAGC 
ATCCGCTGCG TGGTCACAAA TCTAGGCACC GAGGACGGCG ACGCCGCCTT TGTGGCCATC 
CGTGCACGCA TGGTGGCCAA GACGATGGAG AAGCTGGCCT CCAACGTGCC ACTCAATGTG 
TCCACCCTGG CGGTGGCCAA TGTCACACTG CTGCCCTTCA TTGGAGCGCC GAAGGATGCC 
ATTGTGAAGA CGCACGAGAT CTTCTACAAG GCGGAACCGG AACCGCTTCA GGTGCCCGAT 
GTGGTGCCTC TGTGGGTCGT CGTACTGGCC GCCTGTGCCG GAGCCCTCAT CTTCCTGCTG 
CTCGTCTGGC TGCTCTACAA GTGCGGCTTC TTTAACCGCA ACCGGCCAAC GGATCACTCG 
CAGGAGCGGC AGCCACTGAG GAATGGATAC CACGGCGACG AGCACCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523378.2
CDS	258..4448
Translation	MSGDSIHRRRMALHCPITSLILLLIAMSAHGYNIDLPSYVRFRQSSNSMFGFSIAMHKGRSGFYGNQNNVSLIVGAPKFDTSRYQQGVTEAGGVFKCSLNDDDCKLVPFDSKGNNRNVDKEVVDRKSYQWLGATVATGRDSDLVVACAPRYVFHTMTPSRAFRIDPVGTCFTSHNFEEFYEVSPCRTNNWGYHRQGSCQAGFSAAINGNGSRLFIGAPGSWYWQGQTYSIPPDAKFPFKPPLYQPFGTGGMASSHDVTRPENQVFSTSESASVNDDSYLGYSMVTGDFDGDRSEDVAIGMPRGGNLVGRIVVNRWNMANIFNITGRQIGEYFGYSLATSDVDGDGLDDLLIGAPMYTDPDNVEGKYDVGRVYILLQGGPTEEKRWTTEHIRDGYHSKGRFGLALTTLGDVNGDGYGDFAVGAPYDGPEGRGVVYIFHGSPMGPLAKPSQIIKSEQLVEGAPYPRTFGFALSGGLDMDGNTYPDLAVGAYSSDQVFIFKSRPVAAVNAETSFASNSKLISLDDRSCQLVRDHKKVPCMLLTTCWSYTGRYLPEQLDFDVSWLLDAKKLLNPRMFFLRDEGKNIRNQTIRLNYGQKYCLNETVYLLDKVQDKLTPLEVEARYNLRSSRPLDPMVRHRRSILEPVIDQNREIVLRDAINIQKNCGPDNICEPDLKLKVSTVDKYLFGSPEPLVIEVFISNTNEDAFEAAFYMVTPPDLQFRKLQQLGEKKDTPITCSPPTPENNHTLKCDIGNPLESGKIAHFKISLVPEEKYGSSSSYDFYWEANSTNLEKPGSEYDNKIRQSVGIWVDTDLDIKGTSLPDYQLYKADDYKELENATKEDDIGPQVVHIYEIRNNRPSIIEEAEVFIHLPYETIVGDPLMYLLNQPETGGKIQCDDVAFNEYNLLLDEKLVKKSYLQAQGAIWNSAQVSGQSSSSSSSGGASVHIEKARGEGFVRGVLVSNSTDAGDKLSPKQVEQRRQEDTLEALGDASFVHRDRASQAVQEPQVNQTSFTTYSTSSSSSGSGAPSAQLRGHSTQGHIQMAGPVQHTSSSSSSNYRSWPAQQQQQHQQLLLAGSGGSGLGSPVTFNDKSQFGGRNNNFHTGTLDLGTLNRGNVDNELYRSQGQYQNPSQSLGQSQGQFQANANQGHYQGQNQAQFQARNPGFQGQTSYQGQTQYSGQPGGYQTHHVTYSSGSKPYYGRENEDFYDEDNLQQATPGHWSSSSSSSSSSGTRRLRRSNDKDGATEKPLQIDLNSPCQSARCKSIRCVVTNLGTEDGDAAFVAIRARMVAKTMEKLASNVPLNVSTLAVANVTLLPFIGAPKDAIVKTHEIFYKAEPEPLQVPDVVPLWVVVLAACAGALIFLLLVWLLYKCGFFNRNRPTDHSQERQPLRNGYHGDEHL

Target ORF information:

RefSeq Version	NM_078654.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster inflated (if), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078654.2

ORF Insert Sequence:

ATGAGTGGAG ATTCCATCCA CCGAAGAAGA ATGGCTCTTC ACTGCCCCAT AACGAGCCTG 
ATTCTGCTCC TGATCGCCAT GTCCGCCCAT GGTTACAACA TCGACCTGCC CAGCTACGTC 
CGTTTCCGGC AGTCCAGCAA CTCGATGTTC GGATTCAGCA TTGCGATGCA CAAGGGTCGC 
AGTGGATTCT ACGGCAATCA GAATAATGTC AGTTTGATTG TCGGAGCACC CAAATTCGAC 
ACCTCCCGCT ATCAACAGGG CGTGACCGAG GCAGGAGGCG TTTTCAAATG CAGCCTCAAC 
GACGACGACT GCAAATTGGT GCCATTCGAC TCCAAAGGCA ACAATCGCAA TGTGGACAAG 
GAGGTCGTGG ACAGGAAATC CTATCAATGG TTGGGCGCCA CTGTGGCAAC CGGTCGGGAT 
AGCGACTTGG TTGTGGCCTG TGCACCGCGC TATGTCTTCC ACACGATGAC GCCATCGAGG 
GCGTTCCGCA TTGATCCCGT GGGCACCTGC TTCACCTCGC ACAACTTCGA GGAGTTCTAC 
GAGGTCTCCC CCTGCCGGAC AAACAACTGG GGTTATCATC GCCAGGGCTC GTGTCAGGCT 
GGATTCAGTG CAGCGATCAA CGGGAATGGC TCGCGTCTGT TCATTGGCGC ACCTGGCAGT 
TGGTATTGGC AAGGACAAAC CTACTCGATA CCGCCCGATG CCAAGTTTCC ATTTAAGCCG 
CCTCTTTATC AGCCCTTCGG CACTGGAGGT ATGGCCAGCT CCCACGATGT GACCAGGCCG 
GAGAACCAAG TGTTCTCCAC TTCGGAGAGT GCCTCGGTGA ACGATGACTC CTATTTGGGC 
TACTCGATGG TCACCGGTGA TTTCGATGGC GATCGCAGCG AGGATGTGGC CATTGGAATG 
CCCCGCGGTG GTAATCTCGT GGGAAGGATC GTCGTTAATC GCTGGAACAT GGCGAACATC 
TTTAATATCA CGGGTCGCCA GATTGGCGAA TACTTTGGCT ATTCCTTGGC CACCAGCGAT 
GTGGATGGCG ACGGCCTGGA CGATCTGCTT ATCGGTGCTC CCATGTACAC GGATCCCGAC 
AACGTGGAGG GCAAATACGA TGTGGGCAGG GTGTACATCC TGCTGCAGGG CGGTCCCACC 
GAGGAGAAGC GTTGGACGAC GGAGCACATT CGCGATGGCT ACCACAGCAA GGGTAGATTC 
GGCTTGGCCC TGACCACCTT GGGCGATGTG AATGGCGATG GCTATGGAGA CTTCGCCGTT 
GGTGCCCCCT ACGATGGACC CGAGGGTCGT GGAGTGGTCT ATATCTTCCA TGGCTCGCCG 
ATGGGTCCAC TGGCCAAACC GTCGCAGATC ATTAAGTCCG AGCAGTTGGT GGAGGGAGCT 
CCCTATCCGC GAACATTTGG CTTCGCCTTG TCCGGCGGTC TGGACATGGA CGGCAATACC 
TATCCAGATC TCGCCGTGGG CGCCTACAGC TCCGACCAGG TGTTCATCTT TAAGTCGCGA 
CCTGTGGCCG CTGTTAATGC CGAAACCAGT TTCGCCAGCA ACTCGAAGCT GATCAGTCTG 
GACGACAGGA GCTGTCAGCT GGTCAGGGAT CACAAGAAGG TGCCATGCAT GCTACTGACC 
ACCTGCTGGA GCTACACGGG TCGCTATCTG CCCGAGCAAC TGGACTTCGA TGTGTCCTGG 
CTTCTGGATG CCAAAAAGTT GCTCAATCCT CGCATGTTCT TCCTGAGGGA TGAGGGCAAG 
AACATCAGGA ATCAGACGAT CCGCTTGAAC TATGGCCAGA AGTATTGCCT GAACGAGACC 
GTCTATCTGC TGGACAAGGT GCAGGATAAG TTGACGCCTC TGGAAGTGGA AGCCCGCTAC 
AATCTGCGCA GTAGTCGTCC CTTGGACCCG ATGGTTCGTC ATAGGAGGAG CATCCTTGAG 
CCGGTTATTG ATCAGAACCG CGAGATCGTC CTGCGGGATG CCATCAACAT TCAGAAGAAC 
TGTGGGCCCG ACAATATTTG CGAGCCAGAT CTCAAGCTGA AAGTCAGCAC TGTGGACAAG 
TATCTGTTTG GCTCACCCGA ACCTCTGGTC ATCGAGGTCT TTATCTCGAA CACCAATGAG 
GATGCCTTCG AGGCTGCCTT CTACATGGTC ACCCCGCCGG ATCTGCAGTT CAGGAAGCTG 
CAGCAGCTTG GCGAGAAGAA GGACACGCCC ATCACGTGCT CGCCGCCCAC TCCGGAGAAC 
AACCACACAC TCAAGTGCGA TATTGGAAAC CCGTTAGAGT CTGGCAAGAT TGCACACTTC 
AAGATCAGTT TGGTGCCGGA GGAGAAGTAT GGTAGCTCGT CCAGCTACGA CTTCTACTGG 
GAGGCAAATT CGACGAACTT GGAGAAACCC GGCTCCGAAT ACGACAACAA GATCCGCCAA 
AGCGTGGGTA TCTGGGTGGA CACGGATCTG GACATCAAGG GCACCTCCCT GCCAGACTAC 
CAGCTGTACA AGGCCGACGA CTACAAAGAG TTGGAGAATG CCACCAAGGA GGACGATATT 
GGACCTCAGG TGGTGCACAT CTACGAGATC CGCAACAATC GTCCCTCAAT CATCGAGGAG 
GCGGAGGTCT TCATCCATCT GCCCTACGAG ACAATCGTCG GGGATCCGCT AATGTACCTG 
TTGAATCAAC CAGAAACCGG GGGCAAGATC CAGTGCGACG ATGTGGCATT CAATGAGTAC 
AATCTGCTGC TGGACGAGAA GCTGGTAAAG AAGTCCTATC TGCAGGCCCA GGGCGCCATT 
TGGAATTCGG CCCAGGTCTC TGGCCAATCG TCTAGCTCCT CGTCATCCGG CGGTGCCAGC 
GTTCACATCG AGAAGGCTCG CGGTGAAGGT TTCGTTCGAG GAGTCTTGGT CAGCAATAGC 
ACCGATGCGG GTGACAAATT AAGTCCCAAA CAAGTGGAGC AACGGCGGCA GGAGGACACA 
TTGGAGGCGC TTGGAGATGC ATCCTTCGTG CACCGCGATC GCGCCAGTCA GGCGGTCCAG 
GAACCACAAG TTAATCAGAC CAGCTTCACT ACCTACTCCA CCAGCAGCTC TTCCTCCGGT 
TCGGGTGCTC CATCGGCCCA ACTGCGTGGA CACAGCACAC AGGGTCACAT CCAGATGGCC 
GGACCAGTTC AGCACACGAG CAGCTCCTCC TCTTCCAACT ACCGATCTTG GCCAGCGCAG 
CAGCAGCAGC AGCATCAGCA ACTACTTTTA GCCGGTTCCG GAGGATCTGG CCTTGGATCT 
CCGGTTACCT TTAACGACAA ATCGCAATTT GGTGGCCGTA ACAACAATTT CCATACCGGT 
ACTCTCGATT TGGGCACCCT AAACCGAGGC AATGTGGACA ACGAGCTGTA CAGGAGTCAG 
GGTCAATATC AGAATCCCAG CCAGAGTCTG GGCCAAAGCC AGGGTCAGTT CCAGGCCAAT 
GCCAACCAGG GACATTACCA GGGACAAAAT CAGGCCCAGT TCCAGGCACG TAATCCGGGC 
TTCCAGGGAC AGACTTCCTA CCAGGGACAG ACGCAGTACA GTGGCCAACC AGGTGGCTAT 
CAGACCCACC ATGTTACCTA CTCCTCTGGC AGCAAGCCCT ACTATGGCCG CGAGAATGAG 
GACTTCTACG ACGAGGATAA CCTGCAGCAG GCAACGCCTG GACACTGGAG CAGCAGCAGT 
AGCTCCAGTT CCAGTTCCGG CACACGCCGC CTGCGGAGGT CCAACGATAA GGATGGGGCA 
ACGGAGAAGC CGCTACAGAT CGACCTGAAC TCACCATGCC AGTCGGCCAG GTGTAAGAGC 
ATCCGCTGCG TGGTCACAAA TCTAGGCACC GAGGACGGCG ACGCCGCCTT TGTGGCCATC 
CGTGCACGCA TGGTGGCCAA GACGATGGAG AAGCTGGCCT CCAACGTGCC ACTCAATGTG 
TCCACCCTGG CGGTGGCCAA TGTCACACTG CTGCCCTTCA TTGGAGCGCC GAAGGATGCC 
ATTGTGAAGA CGCACGAGAT CTTCTACAAG GCGGAACCGG AACCGCTTCA GGTGCCCGAT 
GTGGTGCCTC TGTGGGTCGT CGTACTGGCC GCCTGTGCCG GAGCCCTCAT CTTCCTGCTG 
CTCGTCTGGC TGCTCTACAA GTGCGGCTTC TTTAACCGCA ACCGGCCAAC GGATCACTCG 
CAGGAGCGGC AGCCACTGAG GAATGGATAC CACGGCGACG AGCACCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

if ( NM_001169306.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001162777.1 Drosophila melanogaster inflated (if), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa01838
Clone ID Related Accession (Same CDS sequence)	NM_001169307.1 , NM_078654.2 , NM_001169306.2
Accession Version	NM_001169306.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4191bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	inflated, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001169306.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTGGAG ATTCCATCCA CCGAAGAAGA ATGGCTCTTC ACTGCCCCAT AACGAGCCTG 
ATTCTGCTCC TGATCGCCAT GTCCGCCCAT GGTTACAACA TCGACCTGCC CAGCTACGTC 
CGTTTCCGGC AGTCCAGCAA CTCGATGTTC GGATTCAGCA TTGCGATGCA CAAGGGTCGC 
AGTGGATTCT ACGGCAATCA GAATAATGTC AGTTTGATTG TCGGAGCACC CAAATTCGAC 
ACCTCCCGCT ATCAACAGGG CGTGACCGAG GCAGGAGGCG TTTTCAAATG CAGCCTCAAC 
GACGACGACT GCAAATTGGT GCCATTCGAC TCCAAAGGCA ACAATCGCAA TGTGGACAAG 
GAGGTCGTGG ACAGGAAATC CTATCAATGG TTGGGCGCCA CTGTGGCAAC CGGTCGGGAT 
AGCGACTTGG TTGTGGCCTG TGCACCGCGC TATGTCTTCC ACACGATGAC GCCATCGAGG 
GCGTTCCGCA TTGATCCCGT GGGCACCTGC TTCACCTCGC ACAACTTCGA GGAGTTCTAC 
GAGGTCTCCC CCTGCCGGAC AAACAACTGG GGTTATCATC GCCAGGGCTC GTGTCAGGCT 
GGATTCAGTG CAGCGATCAA CGGGAATGGC TCGCGTCTGT TCATTGGCGC ACCTGGCAGT 
TGGTATTGGC AAGGACAAAC CTACTCGATA CCGCCCGATG CCAAGTTTCC ATTTAAGCCG 
CCTCTTTATC AGCCCTTCGG CACTGGAGGT ATGGCCAGCT CCCACGATGT GACCAGGCCG 
GAGAACCAAG TGTTCTCCAC TTCGGAGAGT GCCTCGGTGA ACGATGACTC CTATTTGGGC 
TACTCGATGG TCACCGGTGA TTTCGATGGC GATCGCAGCG AGGATGTGGC CATTGGAATG 
CCCCGCGGTG GTAATCTCGT GGGAAGGATC GTCGTTAATC GCTGGAACAT GGCGAACATC 
TTTAATATCA CGGGTCGCCA GATTGGCGAA TACTTTGGCT ATTCCTTGGC CACCAGCGAT 
GTGGATGGCG ACGGCCTGGA CGATCTGCTT ATCGGTGCTC CCATGTACAC GGATCCCGAC 
AACGTGGAGG GCAAATACGA TGTGGGCAGG GTGTACATCC TGCTGCAGGG CGGTCCCACC 
GAGGAGAAGC GTTGGACGAC GGAGCACATT CGCGATGGCT ACCACAGCAA GGGTAGATTC 
GGCTTGGCCC TGACCACCTT GGGCGATGTG AATGGCGATG GCTATGGAGA CTTCGCCGTT 
GGTGCCCCCT ACGATGGACC CGAGGGTCGT GGAGTGGTCT ATATCTTCCA TGGCTCGCCG 
ATGGGTCCAC TGGCCAAACC GTCGCAGATC ATTAAGTCCG AGCAGTTGGT GGAGGGAGCT 
CCCTATCCGC GAACATTTGG CTTCGCCTTG TCCGGCGGTC TGGACATGGA CGGCAATACC 
TATCCAGATC TCGCCGTGGG CGCCTACAGC TCCGACCAGG TGTTCATCTT TAAGTCGCGA 
CCTGTGGCCG CTGTTAATGC CGAAACCAGT TTCGCCAGCA ACTCGAAGCT GATCAGTCTG 
GACGACAGGA GCTGTCAGCT GGTCAGGGAT CACAAGAAGG TGCCATGCAT GCTACTGACC 
ACCTGCTGGA GCTACACGGG TCGCTATCTG CCCGAGCAAC TGGACTTCGA TGTGTCCTGG 
CTTCTGGATG CCAAAAAGTT GCTCAATCCT CGCATGTTCT TCCTGAGGGA TGAGGGCAAG 
AACATCAGGA ATCAGACGAT CCGCTTGAAC TATGGCCAGA AGTATTGCCT GAACGAGACC 
GTCTATCTGC TGGACAAGGT GCAGGATAAG TTGACGCCTC TGGAAGTGGA AGCCCGCTAC 
AATCTGCGCA GTAGTCGTCC CTTGGACCCG ATGGTTCGTC ATAGGAGGAG CATCCTTGAG 
CCGGTTATTG ATCAGAACCG CGAGATCGTC CTGCGGGATG CCATCAACAT TCAGAAGAAC 
TGTGGGCCCG ACAATATTTG CGAGCCAGAT CTCAAGCTGA AAGTCAGCAC TGTGGACAAG 
TATCTGTTTG GCTCACCCGA ACCTCTGGTC ATCGAGGTCT TTATCTCGAA CACCAATGAG 
GATGCCTTCG AGGCTGCCTT CTACATGGTC ACCCCGCCGG ATCTGCAGTT CAGGAAGCTG 
CAGCAGCTTG GCGAGAAGAA GGACACGCCC ATCACGTGCT CGCCGCCCAC TCCGGAGAAC 
AACCACACAC TCAAGTGCGA TATTGGAAAC CCGTTAGAGT CTGGCAAGAT TGCACACTTC 
AAGATCAGTT TGGTGCCGGA GGAGAAGTAT GGTAGCTCGT CCAGCTACGA CTTCTACTGG 
GAGGCAAATT CGACGAACTT GGAGAAACCC GGCTCCGAAT ACGACAACAA GATCCGCCAA 
AGCGTGGGTA TCTGGGTGGA CACGGATCTG GACATCAAGG GCACCTCCCT GCCAGACTAC 
CAGCTGTACA AGGCCGACGA CTACAAAGAG TTGGAGAATG CCACCAAGGA GGACGATATT 
GGACCTCAGG TGGTGCACAT CTACGAGATC CGCAACAATC GTCCCTCAAT CATCGAGGAG 
GCGGAGGTCT TCATCCATCT GCCCTACGAG ACAATCGTCG GGGATCCGCT AATGTACCTG 
TTGAATCAAC CAGAAACCGG GGGCAAGATC CAGTGCGACG ATGTGGCATT CAATGAGTAC 
AATCTGCTGC TGGACGAGAA GCTGGTAAAG AAGTCCTATC TGCAGGCCCA GGGCGCCATT 
TGGAATTCGG CCCAGGTCTC TGGCCAATCG TCTAGCTCCT CGTCATCCGG CGGTGCCAGC 
GTTCACATCG AGAAGGCTCG CGGTGAAGGT TTCGTTCGAG GAGTCTTGGT CAGCAATAGC 
ACCGATGCGG GTGACAAATT AAGTCCCAAA CAAGTGGAGC AACGGCGGCA GGAGGACACA 
TTGGAGGCGC TTGGAGATGC ATCCTTCGTG CACCGCGATC GCGCCAGTCA GGCGGTCCAG 
GAACCACAAG TTAATCAGAC CAGCTTCACT ACCTACTCCA CCAGCAGCTC TTCCTCCGGT 
TCGGGTGCTC CATCGGCCCA ACTGCGTGGA CACAGCACAC AGGGTCACAT CCAGATGGCC 
GGACCAGTTC AGCACACGAG CAGCTCCTCC TCTTCCAACT ACCGATCTTG GCCAGCGCAG 
CAGCAGCAGC AGCATCAGCA ACTACTTTTA GCCGGTTCCG GAGGATCTGG CCTTGGATCT 
CCGGTTACCT TTAACGACAA ATCGCAATTT GGTGGCCGTA ACAACAATTT CCATACCGGT 
ACTCTCGATT TGGGCACCCT AAACCGAGGC AATGTGGACA ACGAGCTGTA CAGGAGTCAG 
GGTCAATATC AGAATCCCAG CCAGAGTCTG GGCCAAAGCC AGGGTCAGTT CCAGGCCAAT 
GCCAACCAGG GACATTACCA GGGACAAAAT CAGGCCCAGT TCCAGGCACG TAATCCGGGC 
TTCCAGGGAC AGACTTCCTA CCAGGGACAG ACGCAGTACA GTGGCCAACC AGGTGGCTAT 
CAGACCCACC ATGTTACCTA CTCCTCTGGC AGCAAGCCCT ACTATGGCCG CGAGAATGAG 
GACTTCTACG ACGAGGATAA CCTGCAGCAG GCAACGCCTG GACACTGGAG CAGCAGCAGT 
AGCTCCAGTT CCAGTTCCGG CACACGCCGC CTGCGGAGGT CCAACGATAA GGATGGGGCA 
ACGGAGAAGC CGCTACAGAT CGACCTGAAC TCACCATGCC AGTCGGCCAG GTGTAAGAGC 
ATCCGCTGCG TGGTCACAAA TCTAGGCACC GAGGACGGCG ACGCCGCCTT TGTGGCCATC 
CGTGCACGCA TGGTGGCCAA GACGATGGAG AAGCTGGCCT CCAACGTGCC ACTCAATGTG 
TCCACCCTGG CGGTGGCCAA TGTCACACTG CTGCCCTTCA TTGGAGCGCC GAAGGATGCC 
ATTGTGAAGA CGCACGAGAT CTTCTACAAG GCGGAACCGG AACCGCTTCA GGTGCCCGAT 
GTGGTGCCTC TGTGGGTCGT CGTACTGGCC GCCTGTGCCG GAGCCCTCAT CTTCCTGCTG 
CTCGTCTGGC TGCTCTACAA GTGCGGCTTC TTTAACCGCA ACCGGCCAAC GGATCACTCG 
CAGGAGCGGC AGCCACTGAG GAATGGATAC CACGGCGACG AGCACCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162777.1
CDS	217..4407
Translation	MSGDSIHRRRMALHCPITSLILLLIAMSAHGYNIDLPSYVRFRQSSNSMFGFSIAMHKGRSGFYGNQNNVSLIVGAPKFDTSRYQQGVTEAGGVFKCSLNDDDCKLVPFDSKGNNRNVDKEVVDRKSYQWLGATVATGRDSDLVVACAPRYVFHTMTPSRAFRIDPVGTCFTSHNFEEFYEVSPCRTNNWGYHRQGSCQAGFSAAINGNGSRLFIGAPGSWYWQGQTYSIPPDAKFPFKPPLYQPFGTGGMASSHDVTRPENQVFSTSESASVNDDSYLGYSMVTGDFDGDRSEDVAIGMPRGGNLVGRIVVNRWNMANIFNITGRQIGEYFGYSLATSDVDGDGLDDLLIGAPMYTDPDNVEGKYDVGRVYILLQGGPTEEKRWTTEHIRDGYHSKGRFGLALTTLGDVNGDGYGDFAVGAPYDGPEGRGVVYIFHGSPMGPLAKPSQIIKSEQLVEGAPYPRTFGFALSGGLDMDGNTYPDLAVGAYSSDQVFIFKSRPVAAVNAETSFASNSKLISLDDRSCQLVRDHKKVPCMLLTTCWSYTGRYLPEQLDFDVSWLLDAKKLLNPRMFFLRDEGKNIRNQTIRLNYGQKYCLNETVYLLDKVQDKLTPLEVEARYNLRSSRPLDPMVRHRRSILEPVIDQNREIVLRDAINIQKNCGPDNICEPDLKLKVSTVDKYLFGSPEPLVIEVFISNTNEDAFEAAFYMVTPPDLQFRKLQQLGEKKDTPITCSPPTPENNHTLKCDIGNPLESGKIAHFKISLVPEEKYGSSSSYDFYWEANSTNLEKPGSEYDNKIRQSVGIWVDTDLDIKGTSLPDYQLYKADDYKELENATKEDDIGPQVVHIYEIRNNRPSIIEEAEVFIHLPYETIVGDPLMYLLNQPETGGKIQCDDVAFNEYNLLLDEKLVKKSYLQAQGAIWNSAQVSGQSSSSSSSGGASVHIEKARGEGFVRGVLVSNSTDAGDKLSPKQVEQRRQEDTLEALGDASFVHRDRASQAVQEPQVNQTSFTTYSTSSSSSGSGAPSAQLRGHSTQGHIQMAGPVQHTSSSSSSNYRSWPAQQQQQHQQLLLAGSGGSGLGSPVTFNDKSQFGGRNNNFHTGTLDLGTLNRGNVDNELYRSQGQYQNPSQSLGQSQGQFQANANQGHYQGQNQAQFQARNPGFQGQTSYQGQTQYSGQPGGYQTHHVTYSSGSKPYYGRENEDFYDEDNLQQATPGHWSSSSSSSSSSGTRRLRRSNDKDGATEKPLQIDLNSPCQSARCKSIRCVVTNLGTEDGDAAFVAIRARMVAKTMEKLASNVPLNVSTLAVANVTLLPFIGAPKDAIVKTHEIFYKAEPEPLQVPDVVPLWVVVLAACAGALIFLLLVWLLYKCGFFNRNRPTDHSQERQPLRNGYHGDEHL

Target ORF information:

RefSeq Version	NM_001169306.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster inflated (if), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169306.2

ORF Insert Sequence:

ATGAGTGGAG ATTCCATCCA CCGAAGAAGA ATGGCTCTTC ACTGCCCCAT AACGAGCCTG 
ATTCTGCTCC TGATCGCCAT GTCCGCCCAT GGTTACAACA TCGACCTGCC CAGCTACGTC 
CGTTTCCGGC AGTCCAGCAA CTCGATGTTC GGATTCAGCA TTGCGATGCA CAAGGGTCGC 
AGTGGATTCT ACGGCAATCA GAATAATGTC AGTTTGATTG TCGGAGCACC CAAATTCGAC 
ACCTCCCGCT ATCAACAGGG CGTGACCGAG GCAGGAGGCG TTTTCAAATG CAGCCTCAAC 
GACGACGACT GCAAATTGGT GCCATTCGAC TCCAAAGGCA ACAATCGCAA TGTGGACAAG 
GAGGTCGTGG ACAGGAAATC CTATCAATGG TTGGGCGCCA CTGTGGCAAC CGGTCGGGAT 
AGCGACTTGG TTGTGGCCTG TGCACCGCGC TATGTCTTCC ACACGATGAC GCCATCGAGG 
GCGTTCCGCA TTGATCCCGT GGGCACCTGC TTCACCTCGC ACAACTTCGA GGAGTTCTAC 
GAGGTCTCCC CCTGCCGGAC AAACAACTGG GGTTATCATC GCCAGGGCTC GTGTCAGGCT 
GGATTCAGTG CAGCGATCAA CGGGAATGGC TCGCGTCTGT TCATTGGCGC ACCTGGCAGT 
TGGTATTGGC AAGGACAAAC CTACTCGATA CCGCCCGATG CCAAGTTTCC ATTTAAGCCG 
CCTCTTTATC AGCCCTTCGG CACTGGAGGT ATGGCCAGCT CCCACGATGT GACCAGGCCG 
GAGAACCAAG TGTTCTCCAC TTCGGAGAGT GCCTCGGTGA ACGATGACTC CTATTTGGGC 
TACTCGATGG TCACCGGTGA TTTCGATGGC GATCGCAGCG AGGATGTGGC CATTGGAATG 
CCCCGCGGTG GTAATCTCGT GGGAAGGATC GTCGTTAATC GCTGGAACAT GGCGAACATC 
TTTAATATCA CGGGTCGCCA GATTGGCGAA TACTTTGGCT ATTCCTTGGC CACCAGCGAT 
GTGGATGGCG ACGGCCTGGA CGATCTGCTT ATCGGTGCTC CCATGTACAC GGATCCCGAC 
AACGTGGAGG GCAAATACGA TGTGGGCAGG GTGTACATCC TGCTGCAGGG CGGTCCCACC 
GAGGAGAAGC GTTGGACGAC GGAGCACATT CGCGATGGCT ACCACAGCAA GGGTAGATTC 
GGCTTGGCCC TGACCACCTT GGGCGATGTG AATGGCGATG GCTATGGAGA CTTCGCCGTT 
GGTGCCCCCT ACGATGGACC CGAGGGTCGT GGAGTGGTCT ATATCTTCCA TGGCTCGCCG 
ATGGGTCCAC TGGCCAAACC GTCGCAGATC ATTAAGTCCG AGCAGTTGGT GGAGGGAGCT 
CCCTATCCGC GAACATTTGG CTTCGCCTTG TCCGGCGGTC TGGACATGGA CGGCAATACC 
TATCCAGATC TCGCCGTGGG CGCCTACAGC TCCGACCAGG TGTTCATCTT TAAGTCGCGA 
CCTGTGGCCG CTGTTAATGC CGAAACCAGT TTCGCCAGCA ACTCGAAGCT GATCAGTCTG 
GACGACAGGA GCTGTCAGCT GGTCAGGGAT CACAAGAAGG TGCCATGCAT GCTACTGACC 
ACCTGCTGGA GCTACACGGG TCGCTATCTG CCCGAGCAAC TGGACTTCGA TGTGTCCTGG 
CTTCTGGATG CCAAAAAGTT GCTCAATCCT CGCATGTTCT TCCTGAGGGA TGAGGGCAAG 
AACATCAGGA ATCAGACGAT CCGCTTGAAC TATGGCCAGA AGTATTGCCT GAACGAGACC 
GTCTATCTGC TGGACAAGGT GCAGGATAAG TTGACGCCTC TGGAAGTGGA AGCCCGCTAC 
AATCTGCGCA GTAGTCGTCC CTTGGACCCG ATGGTTCGTC ATAGGAGGAG CATCCTTGAG 
CCGGTTATTG ATCAGAACCG CGAGATCGTC CTGCGGGATG CCATCAACAT TCAGAAGAAC 
TGTGGGCCCG ACAATATTTG CGAGCCAGAT CTCAAGCTGA AAGTCAGCAC TGTGGACAAG 
TATCTGTTTG GCTCACCCGA ACCTCTGGTC ATCGAGGTCT TTATCTCGAA CACCAATGAG 
GATGCCTTCG AGGCTGCCTT CTACATGGTC ACCCCGCCGG ATCTGCAGTT CAGGAAGCTG 
CAGCAGCTTG GCGAGAAGAA GGACACGCCC ATCACGTGCT CGCCGCCCAC TCCGGAGAAC 
AACCACACAC TCAAGTGCGA TATTGGAAAC CCGTTAGAGT CTGGCAAGAT TGCACACTTC 
AAGATCAGTT TGGTGCCGGA GGAGAAGTAT GGTAGCTCGT CCAGCTACGA CTTCTACTGG 
GAGGCAAATT CGACGAACTT GGAGAAACCC GGCTCCGAAT ACGACAACAA GATCCGCCAA 
AGCGTGGGTA TCTGGGTGGA CACGGATCTG GACATCAAGG GCACCTCCCT GCCAGACTAC 
CAGCTGTACA AGGCCGACGA CTACAAAGAG TTGGAGAATG CCACCAAGGA GGACGATATT 
GGACCTCAGG TGGTGCACAT CTACGAGATC CGCAACAATC GTCCCTCAAT CATCGAGGAG 
GCGGAGGTCT TCATCCATCT GCCCTACGAG ACAATCGTCG GGGATCCGCT AATGTACCTG 
TTGAATCAAC CAGAAACCGG GGGCAAGATC CAGTGCGACG ATGTGGCATT CAATGAGTAC 
AATCTGCTGC TGGACGAGAA GCTGGTAAAG AAGTCCTATC TGCAGGCCCA GGGCGCCATT 
TGGAATTCGG CCCAGGTCTC TGGCCAATCG TCTAGCTCCT CGTCATCCGG CGGTGCCAGC 
GTTCACATCG AGAAGGCTCG CGGTGAAGGT TTCGTTCGAG GAGTCTTGGT CAGCAATAGC 
ACCGATGCGG GTGACAAATT AAGTCCCAAA CAAGTGGAGC AACGGCGGCA GGAGGACACA 
TTGGAGGCGC TTGGAGATGC ATCCTTCGTG CACCGCGATC GCGCCAGTCA GGCGGTCCAG 
GAACCACAAG TTAATCAGAC CAGCTTCACT ACCTACTCCA CCAGCAGCTC TTCCTCCGGT 
TCGGGTGCTC CATCGGCCCA ACTGCGTGGA CACAGCACAC AGGGTCACAT CCAGATGGCC 
GGACCAGTTC AGCACACGAG CAGCTCCTCC TCTTCCAACT ACCGATCTTG GCCAGCGCAG 
CAGCAGCAGC AGCATCAGCA ACTACTTTTA GCCGGTTCCG GAGGATCTGG CCTTGGATCT 
CCGGTTACCT TTAACGACAA ATCGCAATTT GGTGGCCGTA ACAACAATTT CCATACCGGT 
ACTCTCGATT TGGGCACCCT AAACCGAGGC AATGTGGACA ACGAGCTGTA CAGGAGTCAG 
GGTCAATATC AGAATCCCAG CCAGAGTCTG GGCCAAAGCC AGGGTCAGTT CCAGGCCAAT 
GCCAACCAGG GACATTACCA GGGACAAAAT CAGGCCCAGT TCCAGGCACG TAATCCGGGC 
TTCCAGGGAC AGACTTCCTA CCAGGGACAG ACGCAGTACA GTGGCCAACC AGGTGGCTAT 
CAGACCCACC ATGTTACCTA CTCCTCTGGC AGCAAGCCCT ACTATGGCCG CGAGAATGAG 
GACTTCTACG ACGAGGATAA CCTGCAGCAG GCAACGCCTG GACACTGGAG CAGCAGCAGT 
AGCTCCAGTT CCAGTTCCGG CACACGCCGC CTGCGGAGGT CCAACGATAA GGATGGGGCA 
ACGGAGAAGC CGCTACAGAT CGACCTGAAC TCACCATGCC AGTCGGCCAG GTGTAAGAGC 
ATCCGCTGCG TGGTCACAAA TCTAGGCACC GAGGACGGCG ACGCCGCCTT TGTGGCCATC 
CGTGCACGCA TGGTGGCCAA GACGATGGAG AAGCTGGCCT CCAACGTGCC ACTCAATGTG 
TCCACCCTGG CGGTGGCCAA TGTCACACTG CTGCCCTTCA TTGGAGCGCC GAAGGATGCC 
ATTGTGAAGA CGCACGAGAT CTTCTACAAG GCGGAACCGG AACCGCTTCA GGTGCCCGAT 
GTGGTGCCTC TGTGGGTCGT CGTACTGGCC GCCTGTGCCG GAGCCCTCAT CTTCCTGCTG 
CTCGTCTGGC TGCTCTACAA GTGCGGCTTC TTTAACCGCA ACCGGCCAAC GGATCACTCG 
CAGGAGCGGC AGCCACTGAG GAATGGATAC CACGGCGACG AGCACCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

if ( NM_167544.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_728021.2 Drosophila melanogaster inflated (if), transcript variant C, mRNA.

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CloneID	OFa03066
Clone ID Related Accession (Same CDS sequence)	NM_167544.2
Accession Version	NM_167544.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	inflated, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Dec 17, 2009 this sequence version replaced NM_167544.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTGGAG ATTCCATCCA CCGAAGAAGA ATGGCTCTTC ACTGCCCCAT AACGAGCCTG 
ATTCTGCTCC TGATCGCCAT GTCCGCCCAT GGTTACAACA TCGACCTGCC CAGCTACGTC 
CGTTTCCGGC AGTCCAGCAA CTCGATGTTC GGATTCAGCA TTGCGATGCA CAAGGGTCGC 
AGTGGATTCT ACGGCAATCA GAATAATGTC AGTTTGATTG TCGGAGCACC CAAATTCGAC 
ACCTCCCGCT ATCAACAGGG CGTGACCGAG GCAGGAGGCG TTTTCAAATG CAGCCTCAAC 
GACGACGACT GCAAATTGGT GCCATTCGAC TCCAAAGGCA ACAATCGCAA TGTGGACAAG 
GAGGTCGTGG ACAGGAAATC CTATCAATGG TTGGGCGCCA CTGTGGCAAC CGGTCGGGAT 
AGCGACTTGG TTGTGGCCTG TGCACCGCGC TATGTCTTCC ACACGATGAC GCCATCGAGG 
GCGTTCCGCA TTGATCCCGT GGGCACCTGC TTCACCTCGC ACAACTTCGA GGAGTTCTAC 
GAGGTCTCCC CCTGCCGGAC AAACAACTGG GGTTATCATC GCCAGGGCTC GTGTCAGGCT 
GGATTCAGTG CAGCGATCAA CGGGAATGGC TCGCGTCTGT TCATTGGCGC ACCTGGCAGT 
TGGTATTGGC AAGGTATGGC CAGCTCCCAC GATGTGACCA GGCCGGAGAA CCAAGTGTTC 
TCCACTTCGG AGAGTGCCTC GGTGAACGAT GACTCCTATT TGGGCTACTC GATGGTCACC 
GGTGATTTCG ATGGCGATCG CAGCGAGGAT GTGGCCATTG GAATGCCCCG CGGTGGTAAT 
CTCGTGGGAA GGATCGTCGT TAATCGCTGG AACATGGCGA ACATCTTTAA TATCACGGGT 
CGCCAGATTG GCGAATACTT TGGCTATTCC TTGGCCACCA GCGATGTGGA TGGCGACGGC 
CTGGACGATC TGCTTATCGG TGCTCCCATG TACACGGATC CCGACAACGT GGAGGGCAAA 
TACGATGTGG GCAGGGTGTA CATCCTGCTG CAGGGCGGTC CCACCGAGGA GAAGCGTTGG 
ACGACGGAGC ACATTCGCGA TGGCTACCAC AGCAAGGGTA GATTCGGCTT GGCCCTGACC 
ACCTTGGGCG ATGTGAATGG CGATGGCTAT GGAGACTTCG CCGTTGGTGC CCCCTACGAT 
GGACCCGAGG GTCGTGGAGT GGTCTATATC TTCCATGGCT CGCCGATGGG TCCACTGGCC 
AAACCGTCGC AGATCATTAA GTCCGAGCAG TTGGTGGAGG GAGCTCCCTA TCCGCGAACA 
TTTGGCTTCG CCTTGTCCGG CGGTCTGGAC ATGGACGGCA ATACCTATCC AGATCTCGCC 
GTGGGCGCCT ACAGCTCCGA CCAGGTGTTC ATCTTTAAGT CGCGACCTGT GGCCGCTGTT 
AATGCCGAAA CCAGTTTCGC CAGCAACTCG AAGCTGATCA GTCTGGACGA CAGGAGCTGT 
CAGCTGGTCA GGGATCACAA GAAGGTGCCA TGCATGCTAC TGACCACCTG CTGGAGCTAC 
ACGGGTCGCT ATCTGCCCGA GCAACTGGAC TTCGATGTGT CCTGGCTTCT GGATGCCAAA 
AAGTTGCTCA ATCCTCGCAT GTTCTTCCTG AGGGATGAGG GCAAGAACAT CAGGAATCAG 
ACGATCCGCT TGAACTATGG CCAGAAGTAT TGCCTGAACG AGACCGTCTA TCTGCTGGAC 
AAGGTGCAGG ATAAGTTGAC GCCTCTGGAA GTGGAAGCCC GCTACAATCT GCGCAGTAGT 
CGTCCCTTGG ACCCGATGGT TCGTCATAGG AGGAGCATCC TTGAGCCGGT TATTGATCAG 
AACCGCGAGA TCGTCCTGCG GGATGCCATC AACATTCAGA AGAACTGTGG GCCCGACAAT 
ATTTGCGAGC CAGATCTCAA GCTGAAAGTC AGCACTGTGG ACAAGTATCT GTTTGGCTCA 
CCCGAACCTC TGGTCATCGA GGTCTTTATC TCGAACACCA ATGAGGATGC CTTCGAGGCT 
GCCTTCTACA TGGTCACCCC GCCGGATCTG CAGTTCAGGA AGCTGCAGCA GCTTGGCGAG 
AAGAAGGACA CGCCCATCAC GTGCTCGCCG CCCACTCCGG AGAACAACCA CACACTCAAG 
TGCGATATTG GAAACCCGTT AGAGTCTGGC AAGATTGCAC ACTTCAAGAT CAGTTTGGTG 
CCGGAGGAGA AGTATGGTAG CTCGTCCAGC TACGACTTCT ACTGGGAGGC AAATTCGACG 
AACTTGGAGA AACCCGGCTC CGAATACGAC AACAAGATCC GCCAAAGCGT GGGTATCTGG 
GTGGACACGG ATCTGGACAT CAAGGGCACC TCCCTGCCAG ACTACCAGCT GTACAAGGCC 
GACGACTACA AAGAGTTGGA GAATGCCACC AAGGAGGACG ATATTGGACC TCAGGTGGTG 
CACATCTACG AGATCCGCAA CAATCGTCCC TCAATCATCG AGGAGGCGGA GGTCTTCATC 
CATCTGCCCT ACGAGACAAT CGTCGGGGAT CCGCTAATGT ACCTGTTGAA TCAACCAGAA 
ACCGGGGGCA AGATCCAGTG CGACGATGTG GCATTCAATG AGTACAATCT GCTGCTGGAC 
GAGAAGCTGG TAAAGAAGTC CTATCTGCAG GCCCAGGGCG CCATTTGGAA TTCGGCCCAG 
GTCTCTGGCC AATCGTCTAG CTCCTCGTCA TCCGGCGGTG CCAGCGTTCA CATCGAGAAG 
GCTCGCGGTG AAGGTTTCGT TCGAGGAGTC TTGGTCAGCA ATAGCACCGA TGCGGGTGAC 
AAATTAAGTC CCAAACAAGT GGAGCAACGG CGGCAGGAGG ACACATTGGA GGCGCTTGGA 
GATGCATCCT TCGTGCACCG CGATCGCGCC AGTCAGGCGG TCCAGGAACC ACAAGTTAAT 
CAGACCAGCT TCACTACCTA CTCCACCAGC AGCTCTTCCT CCGGTTCGGG TGCTCCATCG 
GCCCAACTGC GTGGACACAG CACACAGGGT CACATCCAGA TGGCCGGACC AGTTCAGCAC 
ACGAGCAGCT CCTCCTCTTC CAACTACCGA TCTTGGCCAG CGCAGCAGCA GCAGCAGCAT 
CAGCAACTAC TTTTAGCCGG TTCCGGAGGA TCTGGCCTTG GATCTCCGGT TACCTTTAAC 
GACAAATCGC AATTTGGTGG CCGTAACAAC AATTTCCATA CCGGTACTCT CGATTTGGGC 
ACCCTAAACC GAGGCAATGT GGACAACGAG CTGTACAGGA GTCAGGGTCA ATATCAGAAT 
CCCAGCCAGA GTCTGGGCCA AAGCCAGGGT CAGTTCCAGG CCAATGCCAA CCAGGGACAT 
TACCAGGGAC AAAATCAGGC CCAGTTCCAG GCACGTAATC CGGGCTTCCA GGGACAGACT 
TCCTACCAGG GACAGACGCA GTACAGTGGC CAACCAGGTG GCTATCAGAC CCACCATGTT 
ACCTACTCCT CTGGCAGCAA GCCCTACTAT GGCCGCGAGA ATGAGGACTT CTACGACGAG 
GATAACCTGC AGCAGGCAAC GCCTGGACAC TGGAGCAGCA GCAGTAGCTC CAGTTCCAGT 
TCCGGCACAC GCCGCCTGCG GAGGTCCAAC GATAAGGATG GGGCAACGGA GAAGCCGCTA 
CAGATCGACC TGAACTCACC ATGCCAGTCG GCCAGGTGTA AGAGCATCCG CTGCGTGGTC 
ACAAATCTAG GCACCGAGGA CGGCGACGCC GCCTTTGTGG CCATCCGTGC ACGCATGGTG 
GCCAAGACGA TGGAGAAGCT GGCCTCCAAC GTGCCACTCA ATGTGTCCAC CCTGGCGGTG 
GCCAATGTCA CACTGCTGCC CTTCATTGGA GCGCCGAAGG ATGCCATTGT GAAGACGCAC 
GAGATCTTCT ACAAGGCGGA ACCGGAACCG CTTCAGGTGC CCGATGTGGT GCCTCTGTGG 
GTCGTCGTAC TGGCCGCCTG TGCCGGAGCC CTCATCTTCC TGCTGCTCGT CTGGCTGCTC 
TACAAGTGCG GCTTCTTTAA CCGCAACCGG CCAACGGATC ACTCGCAGGA GCGGCAGCCA 
CTGAGGAATG GATACCACGG CGACGAGCAC CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_728021.2
CDS	258..4373
Translation	MSGDSIHRRRMALHCPITSLILLLIAMSAHGYNIDLPSYVRFRQSSNSMFGFSIAMHKGRSGFYGNQNNVSLIVGAPKFDTSRYQQGVTEAGGVFKCSLNDDDCKLVPFDSKGNNRNVDKEVVDRKSYQWLGATVATGRDSDLVVACAPRYVFHTMTPSRAFRIDPVGTCFTSHNFEEFYEVSPCRTNNWGYHRQGSCQAGFSAAINGNGSRLFIGAPGSWYWQGMASSHDVTRPENQVFSTSESASVNDDSYLGYSMVTGDFDGDRSEDVAIGMPRGGNLVGRIVVNRWNMANIFNITGRQIGEYFGYSLATSDVDGDGLDDLLIGAPMYTDPDNVEGKYDVGRVYILLQGGPTEEKRWTTEHIRDGYHSKGRFGLALTTLGDVNGDGYGDFAVGAPYDGPEGRGVVYIFHGSPMGPLAKPSQIIKSEQLVEGAPYPRTFGFALSGGLDMDGNTYPDLAVGAYSSDQVFIFKSRPVAAVNAETSFASNSKLISLDDRSCQLVRDHKKVPCMLLTTCWSYTGRYLPEQLDFDVSWLLDAKKLLNPRMFFLRDEGKNIRNQTIRLNYGQKYCLNETVYLLDKVQDKLTPLEVEARYNLRSSRPLDPMVRHRRSILEPVIDQNREIVLRDAINIQKNCGPDNICEPDLKLKVSTVDKYLFGSPEPLVIEVFISNTNEDAFEAAFYMVTPPDLQFRKLQQLGEKKDTPITCSPPTPENNHTLKCDIGNPLESGKIAHFKISLVPEEKYGSSSSYDFYWEANSTNLEKPGSEYDNKIRQSVGIWVDTDLDIKGTSLPDYQLYKADDYKELENATKEDDIGPQVVHIYEIRNNRPSIIEEAEVFIHLPYETIVGDPLMYLLNQPETGGKIQCDDVAFNEYNLLLDEKLVKKSYLQAQGAIWNSAQVSGQSSSSSSSGGASVHIEKARGEGFVRGVLVSNSTDAGDKLSPKQVEQRRQEDTLEALGDASFVHRDRASQAVQEPQVNQTSFTTYSTSSSSSGSGAPSAQLRGHSTQGHIQMAGPVQHTSSSSSSNYRSWPAQQQQQHQQLLLAGSGGSGLGSPVTFNDKSQFGGRNNNFHTGTLDLGTLNRGNVDNELYRSQGQYQNPSQSLGQSQGQFQANANQGHYQGQNQAQFQARNPGFQGQTSYQGQTQYSGQPGGYQTHHVTYSSGSKPYYGRENEDFYDEDNLQQATPGHWSSSSSSSSSSGTRRLRRSNDKDGATEKPLQIDLNSPCQSARCKSIRCVVTNLGTEDGDAAFVAIRARMVAKTMEKLASNVPLNVSTLAVANVTLLPFIGAPKDAIVKTHEIFYKAEPEPLQVPDVVPLWVVVLAACAGALIFLLLVWLLYKCGFFNRNRPTDHSQERQPLRNGYHGDEHL

Target ORF information:

RefSeq Version	NM_167544.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster inflated (if), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167544.2

ORF Insert Sequence:

ATGAGTGGAG ATTCCATCCA CCGAAGAAGA ATGGCTCTTC ACTGCCCCAT AACGAGCCTG 
ATTCTGCTCC TGATCGCCAT GTCCGCCCAT GGTTACAACA TCGACCTGCC CAGCTACGTC 
CGTTTCCGGC AGTCCAGCAA CTCGATGTTC GGATTCAGCA TTGCGATGCA CAAGGGTCGC 
AGTGGATTCT ACGGCAATCA GAATAATGTC AGTTTGATTG TCGGAGCACC CAAATTCGAC 
ACCTCCCGCT ATCAACAGGG CGTGACCGAG GCAGGAGGCG TTTTCAAATG CAGCCTCAAC 
GACGACGACT GCAAATTGGT GCCATTCGAC TCCAAAGGCA ACAATCGCAA TGTGGACAAG 
GAGGTCGTGG ACAGGAAATC CTATCAATGG TTGGGCGCCA CTGTGGCAAC CGGTCGGGAT 
AGCGACTTGG TTGTGGCCTG TGCACCGCGC TATGTCTTCC ACACGATGAC GCCATCGAGG 
GCGTTCCGCA TTGATCCCGT GGGCACCTGC TTCACCTCGC ACAACTTCGA GGAGTTCTAC 
GAGGTCTCCC CCTGCCGGAC AAACAACTGG GGTTATCATC GCCAGGGCTC GTGTCAGGCT 
GGATTCAGTG CAGCGATCAA CGGGAATGGC TCGCGTCTGT TCATTGGCGC ACCTGGCAGT 
TGGTATTGGC AAGGTATGGC CAGCTCCCAC GATGTGACCA GGCCGGAGAA CCAAGTGTTC 
TCCACTTCGG AGAGTGCCTC GGTGAACGAT GACTCCTATT TGGGCTACTC GATGGTCACC 
GGTGATTTCG ATGGCGATCG CAGCGAGGAT GTGGCCATTG GAATGCCCCG CGGTGGTAAT 
CTCGTGGGAA GGATCGTCGT TAATCGCTGG AACATGGCGA ACATCTTTAA TATCACGGGT 
CGCCAGATTG GCGAATACTT TGGCTATTCC TTGGCCACCA GCGATGTGGA TGGCGACGGC 
CTGGACGATC TGCTTATCGG TGCTCCCATG TACACGGATC CCGACAACGT GGAGGGCAAA 
TACGATGTGG GCAGGGTGTA CATCCTGCTG CAGGGCGGTC CCACCGAGGA GAAGCGTTGG 
ACGACGGAGC ACATTCGCGA TGGCTACCAC AGCAAGGGTA GATTCGGCTT GGCCCTGACC 
ACCTTGGGCG ATGTGAATGG CGATGGCTAT GGAGACTTCG CCGTTGGTGC CCCCTACGAT 
GGACCCGAGG GTCGTGGAGT GGTCTATATC TTCCATGGCT CGCCGATGGG TCCACTGGCC 
AAACCGTCGC AGATCATTAA GTCCGAGCAG TTGGTGGAGG GAGCTCCCTA TCCGCGAACA 
TTTGGCTTCG CCTTGTCCGG CGGTCTGGAC ATGGACGGCA ATACCTATCC AGATCTCGCC 
GTGGGCGCCT ACAGCTCCGA CCAGGTGTTC ATCTTTAAGT CGCGACCTGT GGCCGCTGTT 
AATGCCGAAA CCAGTTTCGC CAGCAACTCG AAGCTGATCA GTCTGGACGA CAGGAGCTGT 
CAGCTGGTCA GGGATCACAA GAAGGTGCCA TGCATGCTAC TGACCACCTG CTGGAGCTAC 
ACGGGTCGCT ATCTGCCCGA GCAACTGGAC TTCGATGTGT CCTGGCTTCT GGATGCCAAA 
AAGTTGCTCA ATCCTCGCAT GTTCTTCCTG AGGGATGAGG GCAAGAACAT CAGGAATCAG 
ACGATCCGCT TGAACTATGG CCAGAAGTAT TGCCTGAACG AGACCGTCTA TCTGCTGGAC 
AAGGTGCAGG ATAAGTTGAC GCCTCTGGAA GTGGAAGCCC GCTACAATCT GCGCAGTAGT 
CGTCCCTTGG ACCCGATGGT TCGTCATAGG AGGAGCATCC TTGAGCCGGT TATTGATCAG 
AACCGCGAGA TCGTCCTGCG GGATGCCATC AACATTCAGA AGAACTGTGG GCCCGACAAT 
ATTTGCGAGC CAGATCTCAA GCTGAAAGTC AGCACTGTGG ACAAGTATCT GTTTGGCTCA 
CCCGAACCTC TGGTCATCGA GGTCTTTATC TCGAACACCA ATGAGGATGC CTTCGAGGCT 
GCCTTCTACA TGGTCACCCC GCCGGATCTG CAGTTCAGGA AGCTGCAGCA GCTTGGCGAG 
AAGAAGGACA CGCCCATCAC GTGCTCGCCG CCCACTCCGG AGAACAACCA CACACTCAAG 
TGCGATATTG GAAACCCGTT AGAGTCTGGC AAGATTGCAC ACTTCAAGAT CAGTTTGGTG 
CCGGAGGAGA AGTATGGTAG CTCGTCCAGC TACGACTTCT ACTGGGAGGC AAATTCGACG 
AACTTGGAGA AACCCGGCTC CGAATACGAC AACAAGATCC GCCAAAGCGT GGGTATCTGG 
GTGGACACGG ATCTGGACAT CAAGGGCACC TCCCTGCCAG ACTACCAGCT GTACAAGGCC 
GACGACTACA AAGAGTTGGA GAATGCCACC AAGGAGGACG ATATTGGACC TCAGGTGGTG 
CACATCTACG AGATCCGCAA CAATCGTCCC TCAATCATCG AGGAGGCGGA GGTCTTCATC 
CATCTGCCCT ACGAGACAAT CGTCGGGGAT CCGCTAATGT ACCTGTTGAA TCAACCAGAA 
ACCGGGGGCA AGATCCAGTG CGACGATGTG GCATTCAATG AGTACAATCT GCTGCTGGAC 
GAGAAGCTGG TAAAGAAGTC CTATCTGCAG GCCCAGGGCG CCATTTGGAA TTCGGCCCAG 
GTCTCTGGCC AATCGTCTAG CTCCTCGTCA TCCGGCGGTG CCAGCGTTCA CATCGAGAAG 
GCTCGCGGTG AAGGTTTCGT TCGAGGAGTC TTGGTCAGCA ATAGCACCGA TGCGGGTGAC 
AAATTAAGTC CCAAACAAGT GGAGCAACGG CGGCAGGAGG ACACATTGGA GGCGCTTGGA 
GATGCATCCT TCGTGCACCG CGATCGCGCC AGTCAGGCGG TCCAGGAACC ACAAGTTAAT 
CAGACCAGCT TCACTACCTA CTCCACCAGC AGCTCTTCCT CCGGTTCGGG TGCTCCATCG 
GCCCAACTGC GTGGACACAG CACACAGGGT CACATCCAGA TGGCCGGACC AGTTCAGCAC 
ACGAGCAGCT CCTCCTCTTC CAACTACCGA TCTTGGCCAG CGCAGCAGCA GCAGCAGCAT 
CAGCAACTAC TTTTAGCCGG TTCCGGAGGA TCTGGCCTTG GATCTCCGGT TACCTTTAAC 
GACAAATCGC AATTTGGTGG CCGTAACAAC AATTTCCATA CCGGTACTCT CGATTTGGGC 
ACCCTAAACC GAGGCAATGT GGACAACGAG CTGTACAGGA GTCAGGGTCA ATATCAGAAT 
CCCAGCCAGA GTCTGGGCCA AAGCCAGGGT CAGTTCCAGG CCAATGCCAA CCAGGGACAT 
TACCAGGGAC AAAATCAGGC CCAGTTCCAG GCACGTAATC CGGGCTTCCA GGGACAGACT 
TCCTACCAGG GACAGACGCA GTACAGTGGC CAACCAGGTG GCTATCAGAC CCACCATGTT 
ACCTACTCCT CTGGCAGCAA GCCCTACTAT GGCCGCGAGA ATGAGGACTT CTACGACGAG 
GATAACCTGC AGCAGGCAAC GCCTGGACAC TGGAGCAGCA GCAGTAGCTC CAGTTCCAGT 
TCCGGCACAC GCCGCCTGCG GAGGTCCAAC GATAAGGATG GGGCAACGGA GAAGCCGCTA 
CAGATCGACC TGAACTCACC ATGCCAGTCG GCCAGGTGTA AGAGCATCCG CTGCGTGGTC 
ACAAATCTAG GCACCGAGGA CGGCGACGCC GCCTTTGTGG CCATCCGTGC ACGCATGGTG 
GCCAAGACGA TGGAGAAGCT GGCCTCCAAC GTGCCACTCA ATGTGTCCAC CCTGGCGGTG 
GCCAATGTCA CACTGCTGCC CTTCATTGGA GCGCCGAAGG ATGCCATTGT GAAGACGCAC 
GAGATCTTCT ACAAGGCGGA ACCGGAACCG CTTCAGGTGC CCGATGTGGT GCCTCTGTGG 
GTCGTCGTAC TGGCCGCCTG TGCCGGAGCC CTCATCTTCC TGCTGCTCGT CTGGCTGCTC 
TACAAGTGCG GCTTCTTTAA CCGCAACCGG CCAACGGATC ACTCGCAGGA GCGGCAGCCA 
CTGAGGAATG GATACCACGG CGACGAGCAC CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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if cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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