mbt cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	mbt
Entrez Gene ID	32631
Full Name	mushroom bodies tiny
Synonyms	CG18582,DmMBT,DmPAK2,Dmel\CG18582,Mbt,PAK,Pak2,STE20,Ste20,anon-EST:fe1G7,dPAK2
General protein information	Preferred Names mushroom bodies tiny Names CG18582 gene product from transcript CG18582-RA CG18582-PA anon-fast-evolving-1G7 mbt-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 14F2-14F2

mRNA and Protein(s)

mRNA	Protein	Name
NM_078651.4	NP_523375.2	mushroom bodies tiny

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PAK4	XP_005616659.1
Gallus gallus (chicken)	PAK4	XP_001233796.3
Homo sapiens (human)	PAK4	NP_001014831.1
Xenopus tropicalis (tropical clawed frog)	pak4	NP_001090671.1
	PAK4	XP_001085295.1
Mus musculus (house mouse)	Pak4	NP_081746.1
Rattus norvegicus (Norway rat)	Pak4	NP_001099708.1
Bos taurus (cattle)	PAK4	NP_001069652.1
Danio rerio (zebrafish)	pak4	NP_001002222.1
Drosophila melanogaster (fruit fly)	mbt	NP_523375.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Pak4 regulates adherens junction accumulation. Pak4 promotes Par3 retention at the zonula adherens.
Title: Pak4 Is Required during Epithelial Polarity Remodeling through Regulating AJ Stability and Bazooka Retention at the ZA.

Mbt regulates neuroblast number throughout postembryonic brain development.
Title: The p21-activated kinase Mbt is a component of the apical protein complex in central brain neuroblasts and controls cell proliferation.

Activation of the human Mbt homologue PAK4 leads to anchorage-independent growth and provide a functional link between a PAK protein and the cadherin-catenin complex.
Title: The Drosophila p21-activated kinase Mbt modulates DE-cadherin-mediated cell adhesion by phosphorylation of Armadillo.

Constitutive activation of Mbt not only disturbs the actin cytoskeleton but also affects adherens junction organisation indicating a requirement of the protein in cell adhesion dependent processes during photoreceptor cell differentiation.
Title: The Drosophila p21 activated kinase Mbt regulates the actin cytoskeleton and adherens junctions to control photoreceptor cell morphogenesis.

role for Mbt as a downstream effector of Cdc42 in photoreceptor cell morphogenesis
Title: Mbt, a Drosophila PAK protein, combines with Cdc42 to regulate photoreceptor cell morphogenesis.

The following mbt gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mbt cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa03039	NM_078651.4 Latest version!	Drosophila melanogaster mushroom bodies tiny (mbt), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mbt ( NM_078651.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523375.2 Drosophila melanogaster mushroom bodies tiny (mbt), mRNA.

Price & Availability↑

CloneID	OFa03039
Clone ID Related Accession (Same CDS sequence)	NM_078651.4
Accession Version	NM_078651.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1920bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mushroom bodies tiny
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_078651.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCTCGA AGAAGAAAAA GAAACCGCTG ATCTCGATGC CCAGCAATTT TGAGCATCGT 
GTGCACACGG GCTTCGACAA GCGGGAGAAC AAATATGTTG GCCTGCCCCT CCAATGGGCG 
TCCATTGTGG GCAACAATCA GATACTCAAG TCCTCCAACC GCCCGCTGCC ACTGGTCGAT 
CCCTCGGAGA TTACGCCCAC CGAGATTCTC GATCTGAAGA CTATTGTGCG TCCGCATCAC 
AACAACAACA AGGCGGACAC CACCTCGCTC AACAGCAGCA GCACAATGAT GATGGGCTCA 
ATGGCGCCGA TGAATCCCAT GGCACCTGGC GCACACCCAA TGATGAGCCA TGGCCCCGGA 
ATGATGATGC CACCCGAGAC GGGCGGCATA GTCCTGCCGA AGACCTCTCA CGTGGCCAGA 
TCCAATTCGC TGCGGAGTTC CAGTCCGCCG CGAGTGCGAC GGGTAGCCAA TGTGCCGCCA 
TCGGTGCCGG AGGAGGAGGG ACCACCGGCA GCTGGAACAC CGGGAGTAGG TGGAGCTAGT 
AGCGGTGGCT TTAAGCCACC CGGTGCCCAT CCCTCCCTGC TCTATAACAG TCAGCATGCG 
CACGCGAATG GAGCAACAGG ACCACTGGCC GTGCGCACGG ATCAAACCAA CCTGCAGCAG 
TATCGCAGCA ATCTGGCCCC GCCATCCGGC GGCTCCATGC CCCAGCAACA GCAGACTTCG 
CCCGTGGGTT CGGTGGCCAG TGGCACGCGA TCCAATCACT CGCACACGAA CAATGGCAAC 
AGCGGCGGCA GCTATCCTCC CATGTATCCC ACAAGCCATC AGCAGCAGCA GCAGCAGCAA 
CAGCAGGCCA AACAGGGTGG CGATCAGAAC CAAAATCCTC TGCATCCGCA TGCTCATCCG 
CATCCGCACC ATCACCAACA TTTGGCCAAG TCGGCGTCCA GGGCATCCAG TTCCAGCGGG 
GGAGCGAGCA GTGCTGCCCA GCAGGCGAGC GGCGCCAGTG GAGGAGCGGC GGGTCAGCCA 
AAGCAGGACC AACGACTCAC CCACGAACAG TTCCGTGCCG CCCTTCAGAT GGTGGTCTCC 
GCCGGTGATC CACGCGAGAA CCTCGACCAC TTTAACAAAA TAGGCGAGGG CTCCACGGGC 
ACCGTATGCA TTGCCACGGA CAAATCCACA GGTCGCCAGG TGGCCGTGAA GAAGATGGAT 
CTGCGCAAAC AGCAGCGACG GGAGCTGCTA TTCAACGAGG TCGTCATCAT GCGGGACTAC 
CATCATCCCA ATATCGTGGA GACATACTCC AGCTTTCTGG TCAACGATGA GCTTTGGGTG 
GTGATGGAGT ACCTCGAGGG CGGCGCCCTC ACCGATATTG TCACCCATTC GCGCATGGAC 
GAGGAGCAGA TAGCCACCGT CTGCAAGCAA TGCTTGAAGG CTTTGGCCTA TTTGCACTCA 
CAGGGCGTCA TTCATCGCGA CATCAAGTCG GACTCGATTC TGCTGGCCGC CGATGGTCGC 
GTGAAGCTAT CGGACTTTGG ATTCTGCGCC CAGGTGTCAC AGGAGCTGCC GAAGCGCAAG 
AGTCTGGTTG GCACGCCGTA TTGGATGTCG CCGGAGGTCA TATCGCGCCT GCCGTACGGC 
CCGGAAGTGG ATATCTGGTC GCTGGGCATC ATGGTCATCG AGATGGTGGA CGGCGAGCCG 
CCGTTCTTCA ACGAACCGCC GCTGCAGGCG ATGCGTCGCA TCCGTGACAT GCAGCCGCCG 
AACCTGAAGA ACGCGCACAA GGTCTCGCCG CGCCTGCAGT CCTTCCTCGA CCGGATGCTG 
GTGCGGGATC CGGCGCAGCG TGCCACCGCC GCCGAGCTGC TGGCCCATCC CTTCCTGCGC 
CAGGCAGGAC CGCCGTCGCT GCTGGTTCCG CTGATGCGCA ATGCGCGACA CCATCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523375.2
CDS	508..2427
Translation	MFSKKKKKPLISMPSNFEHRVHTGFDKRENKYVGLPLQWASIVGNNQILKSSNRPLPLVDPSEITPTEILDLKTIVRPHHNNNKADTTSLNSSSTMMMGSMAPMNPMAPGAHPMMSHGPGMMMPPETGGIVLPKTSHVARSNSLRSSSPPRVRRVANVPPSVPEEEGPPAAGTPGVGGASSGGFKPPGAHPSLLYNSQHAHANGATGPLAVRTDQTNLQQYRSNLAPPSGGSMPQQQQTSPVGSVASGTRSNHSHTNNGNSGGSYPPMYPTSHQQQQQQQQQAKQGGDQNQNPLHPHAHPHPHHHQHLAKSASRASSSSGGASSAAQQASGASGGAAGQPKQDQRLTHEQFRAALQMVVSAGDPRENLDHFNKIGEGSTGTVCIATDKSTGRQVAVKKMDLRKQQRRELLFNEVVIMRDYHHPNIVETYSSFLVNDELWVVMEYLEGGALTDIVTHSRMDEEQIATVCKQCLKALAYLHSQGVIHRDIKSDSILLAADGRVKLSDFGFCAQVSQELPKRKSLVGTPYWMSPEVISRLPYGPEVDIWSLGIMVIEMVDGEPPFFNEPPLQAMRRIRDMQPPNLKNAHKVSPRLQSFLDRMLVRDPAQRATAAELLAHPFLRQAGPPSLLVPLMRNARHHP

Target ORF information:

RefSeq Version	NM_078651.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mushroom bodies tiny (mbt), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078651.4

ORF Insert Sequence:

ATGTTCTCGA AGAAGAAAAA GAAACCGCTG ATCTCGATGC CCAGCAATTT TGAGCATCGT 
GTGCACACGG GCTTCGACAA GCGGGAGAAC AAATATGTTG GCCTGCCCCT CCAATGGGCG 
TCCATTGTGG GCAACAATCA GATACTCAAG TCCTCCAACC GCCCGCTGCC ACTGGTCGAT 
CCCTCGGAGA TTACGCCCAC CGAGATTCTC GATCTGAAGA CTATTGTGCG TCCGCATCAC 
AACAACAACA AGGCGGACAC CACCTCGCTC AACAGCAGCA GCACAATGAT GATGGGCTCA 
ATGGCGCCGA TGAATCCCAT GGCACCTGGC GCACACCCAA TGATGAGCCA TGGCCCCGGA 
ATGATGATGC CACCCGAGAC GGGCGGCATA GTCCTGCCGA AGACCTCTCA CGTGGCCAGA 
TCCAATTCGC TGCGGAGTTC CAGTCCGCCG CGAGTGCGAC GGGTAGCCAA TGTGCCGCCA 
TCGGTGCCGG AGGAGGAGGG ACCACCGGCA GCTGGAACAC CGGGAGTAGG TGGAGCTAGT 
AGCGGTGGCT TTAAGCCACC CGGTGCCCAT CCCTCCCTGC TCTATAACAG TCAGCATGCG 
CACGCGAATG GAGCAACAGG ACCACTGGCC GTGCGCACGG ATCAAACCAA CCTGCAGCAG 
TATCGCAGCA ATCTGGCCCC GCCATCCGGC GGCTCCATGC CCCAGCAACA GCAGACTTCG 
CCCGTGGGTT CGGTGGCCAG TGGCACGCGA TCCAATCACT CGCACACGAA CAATGGCAAC 
AGCGGCGGCA GCTATCCTCC CATGTATCCC ACAAGCCATC AGCAGCAGCA GCAGCAGCAA 
CAGCAGGCCA AACAGGGTGG CGATCAGAAC CAAAATCCTC TGCATCCGCA TGCTCATCCG 
CATCCGCACC ATCACCAACA TTTGGCCAAG TCGGCGTCCA GGGCATCCAG TTCCAGCGGG 
GGAGCGAGCA GTGCTGCCCA GCAGGCGAGC GGCGCCAGTG GAGGAGCGGC GGGTCAGCCA 
AAGCAGGACC AACGACTCAC CCACGAACAG TTCCGTGCCG CCCTTCAGAT GGTGGTCTCC 
GCCGGTGATC CACGCGAGAA CCTCGACCAC TTTAACAAAA TAGGCGAGGG CTCCACGGGC 
ACCGTATGCA TTGCCACGGA CAAATCCACA GGTCGCCAGG TGGCCGTGAA GAAGATGGAT 
CTGCGCAAAC AGCAGCGACG GGAGCTGCTA TTCAACGAGG TCGTCATCAT GCGGGACTAC 
CATCATCCCA ATATCGTGGA GACATACTCC AGCTTTCTGG TCAACGATGA GCTTTGGGTG 
GTGATGGAGT ACCTCGAGGG CGGCGCCCTC ACCGATATTG TCACCCATTC GCGCATGGAC 
GAGGAGCAGA TAGCCACCGT CTGCAAGCAA TGCTTGAAGG CTTTGGCCTA TTTGCACTCA 
CAGGGCGTCA TTCATCGCGA CATCAAGTCG GACTCGATTC TGCTGGCCGC CGATGGTCGC 
GTGAAGCTAT CGGACTTTGG ATTCTGCGCC CAGGTGTCAC AGGAGCTGCC GAAGCGCAAG 
AGTCTGGTTG GCACGCCGTA TTGGATGTCG CCGGAGGTCA TATCGCGCCT GCCGTACGGC 
CCGGAAGTGG ATATCTGGTC GCTGGGCATC ATGGTCATCG AGATGGTGGA CGGCGAGCCG 
CCGTTCTTCA ACGAACCGCC GCTGCAGGCG ATGCGTCGCA TCCGTGACAT GCAGCCGCCG 
AACCTGAAGA ACGCGCACAA GGTCTCGCCG CGCCTGCAGT CCTTCCTCGA CCGGATGCTG 
GTGCGGGATC CGGCGCAGCG TGCCACCGCC GCCGAGCTGC TGGCCCATCC CTTCCTGCGC 
CAGGCAGGAC CGCCGTCGCT GCTGGTTCCG CTGATGCGCA ATGCGCGACA CCATCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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mbt cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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