Nup153 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nup153
Entrez Gene ID	32630
Full Name	Nucleoporin 153kD
Synonyms	CG4453,Dmel\CG4453,NUP153,Nup 153,dNup153,dmNup153,nup153
General protein information	Preferred Names Nucleoporin 153kD Names CG4453 gene product from transcript CG4453-RC CG4453-PA CG4453-PC CG4453-PD Nup153-PA Nup153-PC Nup153-PD nucleoporin 153 nucleoporin 153kD
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 14F2-14F2

mRNA and Protein(s)

mRNA	Protein	Name
NM_132908.5	NP_573136.3	nucleoporin 153kD, isoform A
NM_001144734.2	NP_001138206.1	nucleoporin 153kD, isoform C
NM_001103532.3	NP_001097002.1	nucleoporin 153kD, isoform D

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Nup153 and Megator (Mtor) bind to 25% of the genome in continuous domains extending 10 kb to 500 kb.
Title: Nuclear pore proteins nup153 and megator define transcriptionally active regions in the Drosophila genome.

The FG repeats of Nup153 are necessary for its function in transport, whereas the remainder of the protein maintains pore integrity.
Title: Distinct functions of the Drosophila Nup153 and Nup214 FG domains in nuclear protein transport.

The following Nup153 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nup153 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa03037	NM_132908.5 Latest version!	Drosophila melanogaster nucleoporin 153kD, transcript variant A (Nup153), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$1021.30 $1459.00
OFa03037	NM_001144734.2 Latest version!	Drosophila melanogaster nucleoporin 153kD, transcript variant C (Nup153), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$1021.30 $1459.00
OFa03038	NM_001103532.3 Latest version!	Drosophila melanogaster nucleoporin 153kD, transcript variant D (Nup153), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nup153 ( NM_132908.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_573136.3 Drosophila melanogaster nucleoporin 153kD, transcript variant A (Nup153), mRNA.

Price & Availability↑

CloneID	OFa03037
Clone ID Related Accession (Same CDS sequence)	NM_132908.5 , NM_001144734.2
Accession Version	NM_132908.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nucleoporin 153kD, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_132908.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGATG CACAGGAGCA AAGGGAATCC TCGCAAGCCG AATTGGCCAA AAAGCATCCG 
CTGACGCCGC TCAAGGAGCT GCCTGAAGAA AGCGAGGAGG AGGAGGAGGA AGAGGATGAA 
TCCTCGGCGG GAGCCCTACG GGAGTCTGAC AGCAACAACT CCATCATGGG GAAGATGAAG 
ATGCGCGTGT CCAGCATTCT GCCCGCCTCC TTGAGCGGTT GGTTTTCGCC GTCGTCCAAA 
GATGGGAACG ATGCCTTATC CTCCCCAGCT AATCTCCGCC AGTCACAGCC ACGCCAGTCC 
AATGGACGCC TCACTACCAA GCGGAAACGA GGACGCCGTC GGATAATGCT AGCCGAAGTG 
GATGCCGATG CAGCCGATGA TCTTGATGAC GGCTCCGATG CCAAAGGCCT CAACTATGAG 
GAGGTGGCCC TGGCCGACAA CATAGCCGAA CACGACCTGG CCGCCGAGGA TGAACAGACG 
CGGCGGAGCG AGTACAATGT GTTCCTGCTG CGCAAGCGAG CCGGGGCTGT TGCAGCTGCC 
GGCGGTGACG AGGATGAGGC CGAGGAGGAT GAACTGGAAG AGGACGACGA GGATGGCGAT 
GAGGAGGATG ACGATGAAGA GCAGGAGAAT CTCCAACAGT CCGCCGCCGT GCAGACCAAG 
CGTCGACGCC TGGAGCTGGA GACCCCCGTC AATTTGCCGA ACATGCGTCG TCTGCCCCTT 
TTGTCATCCA CGCCTGCTGC TCCGCTCGCG GCTGCCACCA GCTCCTCCAG CTCCCAGATG 
TACAAGGGCG TCAGCCACAT TGCGCCACAT CGGCGCAATC ACCTCAATTT GTATGGTAGT 
CAACGGCAGC GCGAACCCGC GTACAACTTT TTCACGGGCA ACGAAGCTGC CGAGGGAAGC 
ACCGGGGATC TGCCACACAG CATCCGCCGT TCACTGAACA TACCTTTCGG AGGCAGCAGC 
ACCGCTACCA GCTACAACAA CAGCCTATCC AGCCTTCCTA ACCACAAGCG ACCATCGCTC 
ATAGGCAAGC AAACCCATCG ACGCGATCTT ACCATGGATG AGACGGGGAC TGGCCCGGCC 
ATGAGTTCGG AAGAACATCT CAATCATTTG CTGAGAATAA GCAGGACCAA CAACAATACT 
AGCAACAATA ATAATAATAA TAATAACAAC AACAATGTCA TTGAGACAAA AACAAGACGA 
AGTGAACTGT CTGCGGCCGC AGGAGGTTGC GGTGATTCGC AGTCCGAGAG CGATATGAAC 
GAATACCACG ACAATGGTGA AGGGCACGAC GGACTTCGTC CGTCGCATTA CAACAGCAAC 
AGCAATTTAG AATTTTACGG AAATCTGCAG AGCTCCAAGT CAATATTTAA CAGAAGCAAC 
ACCGCCGCAC AGCAATCGCA TCGGAATTCT ACGTGGTCGC TGAATTCCCT TACCCAACGA 
CGTCGCTTCA ATGCGTCCAT ATACGGCAGT ACGTCGGCAC TGAGCGACAG TCGTCTGCTT 
AGTGGCAGTG CCTCTAACTC AGGCTCTGCA TCTGCCTCTA GCTCGCCCTT TTACCAGGGA 
CGCACCACCT TCGGGGGCAA TTCCGGCAAC AATCGCCTCT TCAGTCGCAG CAATCTTAGC 
TCATCCGCCG CATCCTCAAT GTTGGGACTC AATTCTGCCG GCAGCTCACC GGCGCATCAG 
CTGCATGCTT CCATGACTGG TGGTATTGGA TACGGTATGA AAGCGGTAGA TATGCGACCT 
TCAGACTCCG GCAGTCTTGC GGAGACATCT GTGGGCCAAG GTGGCAGTAA GAAACCCGGT 
ACCGGGCTGT CGAACACCAC GATGCGCATT CTCAACCTAC TTGAAAGCTA CTCCACGCCA 
TTGATCGATG CCAAGCGAAT GGGGAGCAGC ATCAAGGAGC ATCAAAGCAG TCGCCAACAG 
CGCCAGGGTA CGCCAGCCAC CCCGTATCTA CGTTCCACGA GTGCCAGCCG CAATGTTTCG 
GTGCCCAATC ACATCAACGA GTTGGCGGAA CTGCGCTCTA ACAAACTGCT GGTGCCGACG 
ATGCAGCAGC TTCTGGAACG TCGTCGTCTA CACCGCGTTA CACAGAACTC TCGAGATGTG 
GTTCACAGCC AGAACGTTCG TGCTGGTGGG GAAAACAACC AGGAGAAGCC CAAGCCAACT 
GCACCATATG TTGCTCCGAT AGACCAGTCG GCTAACCATA CTCAGCACAC AAACAAGATG 
CGTTCGCGTC TTTCGCACCA GACGCGCAAC AAGGAGACAC GCACGGCGGA AGAGGAAGCA 
CCCCCGCCGC TGGATTTGCC ACAGATCAGC TTTCCGGACA TGGCAAGCGC CCCAAAGTTT 
GATTTGATAA TCAAACCCAC GGTGCCAGTG GTATCAAAAC CATCTACCAC CGACCCAATC 
CAAAGTAGCA AAAGTAGCAA CACCAACCTC TCTACTACAA ACTCCAAGCA GATGCCAAAC 
TTCCTGGCTA ATCCCCAGCC TGCGGCACCA ATTGTTAACT TCGCTGCCAA TGGAAACGTT 
TCTGCAATCA GTAAACCAAG TAAACGCACA TTCACCTTCT CAGAACCCAC ACCTTTGTCG 
AACTTTCAGG AGAACTGCAT CCCCAAGCCG AAGATCAATC GCAAATACAC CTTCTCGGCA 
CCGGCACCAC TGGATGATTT GCGTATAACG AACAAACAGT CGCAGCCAAC CATAAACGGA 
ACGCCGTCAT CCAAGGAGTG GGAGTGTGAT ACGTGTATGG TGCGAAACAA GCCCGAGATC 
AACAAGTGTG TGGCCTGTGA GACGGCCAAG CCGGTAGCAT CTGCGGCTCC TGTCCAAGCA 
CCATTACCAC CATCTACAGC AGCTATTGAC ACACAAAGTT TCGTTGGTTT TGGGGATCGA 
TTCAAGAAAT CGACGACTGC CTGGGAGTGC GATGCCTGCA TGCTGTCCAA TAAGGCTGAA 
GCCAGCAAAT GCATTGCCTG CGAGACTCCA AGGAAAACTG TTGCACCAAA GGTCAACAAC 
TTCTCACCGT TGATTACAAA TGCGAAAAGC AACGAGTGGG AGTGCTCTGT GTGTCTGGTG 
CGCAACAAAG TCGAGGTCAG CAAGTGTGTG GCCTGTGAGT CGGCCAAACC AGGCGCAACT 
ATGGCTCTCC CTGCAACATC TAATATAGCT GTTGCGACTC CCTCTATAAT TACGGATGGC 
TTTGGGGATC GATTCAAGAA ATCAGCGACT GCCTGGGAGT GCGATGCCTG CATGCTGTCC 
AATAAGGCTG AAGCCAGCAA ATGCATTGCC TGCGAAACTC CGCGAAAATC TTCGACTCCA 
ATAGCGAACA GTAGTTACCC GTCGATCAAC AATAATTTGC CAGCCGGATC CGGTTTTGAT 
ATTTCCTTCA CGCGAAAGGC AAACATGTGG GAGTGCCAAA CATGCCTGGT GATGAACAAG 
TCGAGTGATG AGGAATGCAT TGCCTGTCAA ACGCCAAACT CACAGGCGAG AAACAGCAAC 
AGCGAGTCCG CACTGATTTC ATCCATAAGC TCTTCCTCGG CATCGTTCTC CGGATCTTTA 
TCCAGACCCT CATCTAGATC ATCCTCTGGG TCAACCTCAA CCTGTGGGTC AGTTTGTTCG 
GGATCTATCG TCAGCATTAG CTCCACCACC GAAAGCGCCA AAGCATTATC AGCAAAAAAG 
GTGCCCCCCA AGCCGGATGC CGGATTTCAG CAGCTAGTGG CAGCCCAAAA AACATCAACT 
TGGGAATGCG AAGCATGCCT GGCAAAGAAT GATATGTCGC GGAAGACGTG TATCTGCTGT 
GAACAGATGA TGCCGGAAGC ATTTAACCCT GCCGCAACAA CGGCCAACTC CGCAGCCAGT 
TCGGTGCCCA AATTCCGTTT TGGATTTTCT CATGTAAAGG AGGTTGTCAA GCCATCTGTG 
GAAACGACGA CCACACCAGC ACCAACTTCC GCTCAATTTA GCTTCGGTTT CGGCCAAAGC 
AATCAGGGTA AGGATGTGGC GGACAGCAAG AAGACGGAGG CCCCGAAGAC CTTTATGTTT 
GGCGTGAGCA AAGTGGAAGA GCCCAAGACG GTGAGTTTTG GGACAGGCAT CAAGGAAACA 
ACGGCCACAT CGAGCACAGA AGCTACAGCT CCAACTCCAG CTGCAGCAGC ACCAGCACCA 
GTCCAGTTTG TGTTCAAGGC ACCGACTACC GCAACCACGG CCAGTTCACT CACCACCACA 
ATCAGCACCA CCAGCAACGC TCCTGCTTTG GGTGGCTTCA GCTTTGGCGC TCCATCATCG 
TCCTCTACAG TTTCCAGTTC GACGACTAGT ACCAGTGCGA ATCCCGCGGC TGTCAAACCG 
ATGTTCAGCT GGTCCGGAGC TGGCAGTGCC GTGAGCAGCA CCAGTAGCAG CCAGCAGCCA 
GTGGCCAAGG CGCCAACCCT AGGTTTTGGT GTTTCCTCCA GCACCGTAAC AACGACAACA 
ACCTCTACGA AAGTTTTTGC TTTCACACCG GCATCTGGTC TAGATCCGGC AGCAGCCACC 
TCGGCACCAG CTGCAGGTGC CGGCTTCAGT TTTGGAAGTC AGTCAAAGCC AGCAACCACA 
CAGAACACTG GAACCTTCTT CTTTGGCCAG CCAACAGCGG TTGCGCCAGC AACGCCAACC 
AACCCTAGCG TGAGCTCCAT CTTCGGAGCA CCGGCAACAA GTACCACTGC AAGCACCAGC 
GTGAGTGCCA CCACCAGCAC CAGCACCGCC AACGCCATTG CTTCCAGCTT TGCGCCCACC 
TCGACCCCTC AGCTCTTTGG CAATTGGGGT GAAAAGAAAA CGGACTTGAC CACCTTTGGT 
GCTTCTTCCG GTTCGGGAAC GACTACAACG CCTAGCTTCG GCTGGTCGAG CAATGGAGAT 
GCGGCAAAGA GCAACAGCGC TGCGGTCGGC AGTGCGGCGG TGCCTTCGTC TTCGGCCTCC 
ACGATGGCGA CTCCGATATT CGGCAGCAGT TCCATGTTTG GACCATCCAG TAGCAGTAAC 
AACACCACCA GCACCAGCAC CACTAGTCTA CCATTCGGAT CAGCGGCGAC CACTGCCGCA 
ACTACGCCGG CGGGCGGCAA TGCAGCCCTT ACTGGGTTAT TCGGCAATGT GGGCAACTCT 
CTAGCGGGAG TAGGAGCTCC TGTAGCCACA ACACCAGCGG CAACAGCTGC TGCTCCATTG 
ACCAACATCT TTGGCAATCC CACGCCAGTT GCTGCTGCCG CGCCTGTCTT CGGCAGCGGA 
AGCACCATCC CAAGCGCAGG ATTTGGAGCG CCTGCTGCAG CTGCTCCGTT GGCGGCCCCA 
GCGTTACCAG GAGCATTTAA CTTTGGCGGA GCAACTGCAG CCACGCCAGC TGCCTCAAGT 
GCTCCATTTG TATTCGGAAG CAGCACCAAT GAGCCGCTGG CCAAACCGTC GTTCAACTTC 
ACCGGCTCGG CGGCCAGCTC GACTGCTCCT GCGCCAGCCT TCAATTTCAC GGCCAATACG 
GCGGCCACTA ATAATCCCTC CGGTGGCGAT TCCACACCGA ATGCCAATGC ACTGTTCCAA 
TTTAGCGCGA CCTCCACAGC GCCAGCCAAT ATATTTGCCT TCAATCCGCC AGCTGCGGGC 
AACTCGGCGC AGAGTTCCCA GACGGCTCGC CGCAAAATCC GCGCCCCCGT ACGCCGTTTG 
CCGCCCCGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_573136.3
CDS	831..6482
Translation	MEDAQEQRESSQAELAKKHPLTPLKELPEESEEEEEEEDESSAGALRESDSNNSIMGKMKMRVSSILPASLSGWFSPSSKDGNDALSSPANLRQSQPRQSNGRLTTKRKRGRRRIMLAEVDADAADDLDDGSDAKGLNYEEVALADNIAEHDLAAEDEQTRRSEYNVFLLRKRAGAVAAAGGDEDEAEEDELEEDDEDGDEEDDDEEQENLQQSAAVQTKRRRLELETPVNLPNMRRLPLLSSTPAAPLAAATSSSSSQMYKGVSHIAPHRRNHLNLYGSQRQREPAYNFFTGNEAAEGSTGDLPHSIRRSLNIPFGGSSTATSYNNSLSSLPNHKRPSLIGKQTHRRDLTMDETGTGPAMSSEEHLNHLLRISRTNNNTSNNNNNNNNNNNVIETKTRRSELSAAAGGCGDSQSESDMNEYHDNGEGHDGLRPSHYNSNSNLEFYGNLQSSKSIFNRSNTAAQQSHRNSTWSLNSLTQRRRFNASIYGSTSALSDSRLLSGSASNSGSASASSSPFYQGRTTFGGNSGNNRLFSRSNLSSSAASSMLGLNSAGSSPAHQLHASMTGGIGYGMKAVDMRPSDSGSLAETSVGQGGSKKPGTGLSNTTMRILNLLESYSTPLIDAKRMGSSIKEHQSSRQQRQGTPATPYLRSTSASRNVSVPNHINELAELRSNKLLVPTMQQLLERRRLHRVTQNSRDVVHSQNVRAGGENNQEKPKPTAPYVAPIDQSANHTQHTNKMRSRLSHQTRNKETRTAEEEAPPPLDLPQISFPDMASAPKFDLIIKPTVPVVSKPSTTDPIQSSKSSNTNLSTTNSKQMPNFLANPQPAAPIVNFAANGNVSAISKPSKRTFTFSEPTPLSNFQENCIPKPKINRKYTFSAPAPLDDLRITNKQSQPTINGTPSSKEWECDTCMVRNKPEINKCVACETAKPVASAAPVQAPLPPSTAAIDTQSFVGFGDRFKKSTTAWECDACMLSNKAEASKCIACETPRKTVAPKVNNFSPLITNAKSNEWECSVCLVRNKVEVSKCVACESAKPGATMALPATSNIAVATPSIITDGFGDRFKKSATAWECDACMLSNKAEASKCIACETPRKSSTPIANSSYPSINNNLPAGSGFDISFTRKANMWECQTCLVMNKSSDEECIACQTPNSQARNSNSESALISSISSSSASFSGSLSRPSSRSSSGSTSTCGSVCSGSIVSISSTTESAKALSAKKVPPKPDAGFQQLVAAQKTSTWECEACLAKNDMSRKTCICCEQMMPEAFNPAATTANSAASSVPKFRFGFSHVKEVVKPSVETTTTPAPTSAQFSFGFGQSNQGKDVADSKKTEAPKTFMFGVSKVEEPKTVSFGTGIKETTATSSTEATAPTPAAAAPAPVQFVFKAPTTATTASSLTTTISTTSNAPALGGFSFGAPSSSSTVSSSTTSTSANPAAVKPMFSWSGAGSAVSSTSSSQQPVAKAPTLGFGVSSSTVTTTTTSTKVFAFTPASGLDPAAATSAPAAGAGFSFGSQSKPATTQNTGTFFFGQPTAVAPATPTNPSVSSIFGAPATSTTASTSVSATTSTSTANAIASSFAPTSTPQLFGNWGEKKTDLTTFGASSGSGTTTTPSFGWSSNGDAAKSNSAAVGSAAVPSSSASTMATPIFGSSSMFGPSSSSNNTTSTSTTSLPFGSAATTAATTPAGGNAALTGLFGNVGNSLAGVGAPVATTPAATAAAPLTNIFGNPTPVAAAAPVFGSGSTIPSAGFGAPAAAAPLAAPALPGAFNFGGATAATPAASSAPFVFGSSTNEPLAKPSFNFTGSAASSTAPAPAFNFTANTAATNNPSGGDSTPNANALFQFSATSTAPANIFAFNPPAAGNSAQSSQTARRKIRAPVRRLPPR

Target ORF information:

RefSeq Version	NM_132908.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nucleoporin 153kD, transcript variant A (Nup153), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132908.5

ORF Insert Sequence:

ATGGAGGATG CACAGGAGCA AAGGGAATCC TCGCAAGCCG AATTGGCCAA AAAGCATCCG 
CTGACGCCGC TCAAGGAGCT GCCTGAAGAA AGCGAGGAGG AGGAGGAGGA AGAGGATGAA 
TCCTCGGCGG GAGCCCTACG GGAGTCTGAC AGCAACAACT CCATCATGGG GAAGATGAAG 
ATGCGCGTGT CCAGCATTCT GCCCGCCTCC TTGAGCGGTT GGTTTTCGCC GTCGTCCAAA 
GATGGGAACG ATGCCTTATC CTCCCCAGCT AATCTCCGCC AGTCACAGCC ACGCCAGTCC 
AATGGACGCC TCACTACCAA GCGGAAACGA GGACGCCGTC GGATAATGCT AGCCGAAGTG 
GATGCCGATG CAGCCGATGA TCTTGATGAC GGCTCCGATG CCAAAGGCCT CAACTATGAG 
GAGGTGGCCC TGGCCGACAA CATAGCCGAA CACGACCTGG CCGCCGAGGA TGAACAGACG 
CGGCGGAGCG AGTACAATGT GTTCCTGCTG CGCAAGCGAG CCGGGGCTGT TGCAGCTGCC 
GGCGGTGACG AGGATGAGGC CGAGGAGGAT GAACTGGAAG AGGACGACGA GGATGGCGAT 
GAGGAGGATG ACGATGAAGA GCAGGAGAAT CTCCAACAGT CCGCCGCCGT GCAGACCAAG 
CGTCGACGCC TGGAGCTGGA GACCCCCGTC AATTTGCCGA ACATGCGTCG TCTGCCCCTT 
TTGTCATCCA CGCCTGCTGC TCCGCTCGCG GCTGCCACCA GCTCCTCCAG CTCCCAGATG 
TACAAGGGCG TCAGCCACAT TGCGCCACAT CGGCGCAATC ACCTCAATTT GTATGGTAGT 
CAACGGCAGC GCGAACCCGC GTACAACTTT TTCACGGGCA ACGAAGCTGC CGAGGGAAGC 
ACCGGGGATC TGCCACACAG CATCCGCCGT TCACTGAACA TACCTTTCGG AGGCAGCAGC 
ACCGCTACCA GCTACAACAA CAGCCTATCC AGCCTTCCTA ACCACAAGCG ACCATCGCTC 
ATAGGCAAGC AAACCCATCG ACGCGATCTT ACCATGGATG AGACGGGGAC TGGCCCGGCC 
ATGAGTTCGG AAGAACATCT CAATCATTTG CTGAGAATAA GCAGGACCAA CAACAATACT 
AGCAACAATA ATAATAATAA TAATAACAAC AACAATGTCA TTGAGACAAA AACAAGACGA 
AGTGAACTGT CTGCGGCCGC AGGAGGTTGC GGTGATTCGC AGTCCGAGAG CGATATGAAC 
GAATACCACG ACAATGGTGA AGGGCACGAC GGACTTCGTC CGTCGCATTA CAACAGCAAC 
AGCAATTTAG AATTTTACGG AAATCTGCAG AGCTCCAAGT CAATATTTAA CAGAAGCAAC 
ACCGCCGCAC AGCAATCGCA TCGGAATTCT ACGTGGTCGC TGAATTCCCT TACCCAACGA 
CGTCGCTTCA ATGCGTCCAT ATACGGCAGT ACGTCGGCAC TGAGCGACAG TCGTCTGCTT 
AGTGGCAGTG CCTCTAACTC AGGCTCTGCA TCTGCCTCTA GCTCGCCCTT TTACCAGGGA 
CGCACCACCT TCGGGGGCAA TTCCGGCAAC AATCGCCTCT TCAGTCGCAG CAATCTTAGC 
TCATCCGCCG CATCCTCAAT GTTGGGACTC AATTCTGCCG GCAGCTCACC GGCGCATCAG 
CTGCATGCTT CCATGACTGG TGGTATTGGA TACGGTATGA AAGCGGTAGA TATGCGACCT 
TCAGACTCCG GCAGTCTTGC GGAGACATCT GTGGGCCAAG GTGGCAGTAA GAAACCCGGT 
ACCGGGCTGT CGAACACCAC GATGCGCATT CTCAACCTAC TTGAAAGCTA CTCCACGCCA 
TTGATCGATG CCAAGCGAAT GGGGAGCAGC ATCAAGGAGC ATCAAAGCAG TCGCCAACAG 
CGCCAGGGTA CGCCAGCCAC CCCGTATCTA CGTTCCACGA GTGCCAGCCG CAATGTTTCG 
GTGCCCAATC ACATCAACGA GTTGGCGGAA CTGCGCTCTA ACAAACTGCT GGTGCCGACG 
ATGCAGCAGC TTCTGGAACG TCGTCGTCTA CACCGCGTTA CACAGAACTC TCGAGATGTG 
GTTCACAGCC AGAACGTTCG TGCTGGTGGG GAAAACAACC AGGAGAAGCC CAAGCCAACT 
GCACCATATG TTGCTCCGAT AGACCAGTCG GCTAACCATA CTCAGCACAC AAACAAGATG 
CGTTCGCGTC TTTCGCACCA GACGCGCAAC AAGGAGACAC GCACGGCGGA AGAGGAAGCA 
CCCCCGCCGC TGGATTTGCC ACAGATCAGC TTTCCGGACA TGGCAAGCGC CCCAAAGTTT 
GATTTGATAA TCAAACCCAC GGTGCCAGTG GTATCAAAAC CATCTACCAC CGACCCAATC 
CAAAGTAGCA AAAGTAGCAA CACCAACCTC TCTACTACAA ACTCCAAGCA GATGCCAAAC 
TTCCTGGCTA ATCCCCAGCC TGCGGCACCA ATTGTTAACT TCGCTGCCAA TGGAAACGTT 
TCTGCAATCA GTAAACCAAG TAAACGCACA TTCACCTTCT CAGAACCCAC ACCTTTGTCG 
AACTTTCAGG AGAACTGCAT CCCCAAGCCG AAGATCAATC GCAAATACAC CTTCTCGGCA 
CCGGCACCAC TGGATGATTT GCGTATAACG AACAAACAGT CGCAGCCAAC CATAAACGGA 
ACGCCGTCAT CCAAGGAGTG GGAGTGTGAT ACGTGTATGG TGCGAAACAA GCCCGAGATC 
AACAAGTGTG TGGCCTGTGA GACGGCCAAG CCGGTAGCAT CTGCGGCTCC TGTCCAAGCA 
CCATTACCAC CATCTACAGC AGCTATTGAC ACACAAAGTT TCGTTGGTTT TGGGGATCGA 
TTCAAGAAAT CGACGACTGC CTGGGAGTGC GATGCCTGCA TGCTGTCCAA TAAGGCTGAA 
GCCAGCAAAT GCATTGCCTG CGAGACTCCA AGGAAAACTG TTGCACCAAA GGTCAACAAC 
TTCTCACCGT TGATTACAAA TGCGAAAAGC AACGAGTGGG AGTGCTCTGT GTGTCTGGTG 
CGCAACAAAG TCGAGGTCAG CAAGTGTGTG GCCTGTGAGT CGGCCAAACC AGGCGCAACT 
ATGGCTCTCC CTGCAACATC TAATATAGCT GTTGCGACTC CCTCTATAAT TACGGATGGC 
TTTGGGGATC GATTCAAGAA ATCAGCGACT GCCTGGGAGT GCGATGCCTG CATGCTGTCC 
AATAAGGCTG AAGCCAGCAA ATGCATTGCC TGCGAAACTC CGCGAAAATC TTCGACTCCA 
ATAGCGAACA GTAGTTACCC GTCGATCAAC AATAATTTGC CAGCCGGATC CGGTTTTGAT 
ATTTCCTTCA CGCGAAAGGC AAACATGTGG GAGTGCCAAA CATGCCTGGT GATGAACAAG 
TCGAGTGATG AGGAATGCAT TGCCTGTCAA ACGCCAAACT CACAGGCGAG AAACAGCAAC 
AGCGAGTCCG CACTGATTTC ATCCATAAGC TCTTCCTCGG CATCGTTCTC CGGATCTTTA 
TCCAGACCCT CATCTAGATC ATCCTCTGGG TCAACCTCAA CCTGTGGGTC AGTTTGTTCG 
GGATCTATCG TCAGCATTAG CTCCACCACC GAAAGCGCCA AAGCATTATC AGCAAAAAAG 
GTGCCCCCCA AGCCGGATGC CGGATTTCAG CAGCTAGTGG CAGCCCAAAA AACATCAACT 
TGGGAATGCG AAGCATGCCT GGCAAAGAAT GATATGTCGC GGAAGACGTG TATCTGCTGT 
GAACAGATGA TGCCGGAAGC ATTTAACCCT GCCGCAACAA CGGCCAACTC CGCAGCCAGT 
TCGGTGCCCA AATTCCGTTT TGGATTTTCT CATGTAAAGG AGGTTGTCAA GCCATCTGTG 
GAAACGACGA CCACACCAGC ACCAACTTCC GCTCAATTTA GCTTCGGTTT CGGCCAAAGC 
AATCAGGGTA AGGATGTGGC GGACAGCAAG AAGACGGAGG CCCCGAAGAC CTTTATGTTT 
GGCGTGAGCA AAGTGGAAGA GCCCAAGACG GTGAGTTTTG GGACAGGCAT CAAGGAAACA 
ACGGCCACAT CGAGCACAGA AGCTACAGCT CCAACTCCAG CTGCAGCAGC ACCAGCACCA 
GTCCAGTTTG TGTTCAAGGC ACCGACTACC GCAACCACGG CCAGTTCACT CACCACCACA 
ATCAGCACCA CCAGCAACGC TCCTGCTTTG GGTGGCTTCA GCTTTGGCGC TCCATCATCG 
TCCTCTACAG TTTCCAGTTC GACGACTAGT ACCAGTGCGA ATCCCGCGGC TGTCAAACCG 
ATGTTCAGCT GGTCCGGAGC TGGCAGTGCC GTGAGCAGCA CCAGTAGCAG CCAGCAGCCA 
GTGGCCAAGG CGCCAACCCT AGGTTTTGGT GTTTCCTCCA GCACCGTAAC AACGACAACA 
ACCTCTACGA AAGTTTTTGC TTTCACACCG GCATCTGGTC TAGATCCGGC AGCAGCCACC 
TCGGCACCAG CTGCAGGTGC CGGCTTCAGT TTTGGAAGTC AGTCAAAGCC AGCAACCACA 
CAGAACACTG GAACCTTCTT CTTTGGCCAG CCAACAGCGG TTGCGCCAGC AACGCCAACC 
AACCCTAGCG TGAGCTCCAT CTTCGGAGCA CCGGCAACAA GTACCACTGC AAGCACCAGC 
GTGAGTGCCA CCACCAGCAC CAGCACCGCC AACGCCATTG CTTCCAGCTT TGCGCCCACC 
TCGACCCCTC AGCTCTTTGG CAATTGGGGT GAAAAGAAAA CGGACTTGAC CACCTTTGGT 
GCTTCTTCCG GTTCGGGAAC GACTACAACG CCTAGCTTCG GCTGGTCGAG CAATGGAGAT 
GCGGCAAAGA GCAACAGCGC TGCGGTCGGC AGTGCGGCGG TGCCTTCGTC TTCGGCCTCC 
ACGATGGCGA CTCCGATATT CGGCAGCAGT TCCATGTTTG GACCATCCAG TAGCAGTAAC 
AACACCACCA GCACCAGCAC CACTAGTCTA CCATTCGGAT CAGCGGCGAC CACTGCCGCA 
ACTACGCCGG CGGGCGGCAA TGCAGCCCTT ACTGGGTTAT TCGGCAATGT GGGCAACTCT 
CTAGCGGGAG TAGGAGCTCC TGTAGCCACA ACACCAGCGG CAACAGCTGC TGCTCCATTG 
ACCAACATCT TTGGCAATCC CACGCCAGTT GCTGCTGCCG CGCCTGTCTT CGGCAGCGGA 
AGCACCATCC CAAGCGCAGG ATTTGGAGCG CCTGCTGCAG CTGCTCCGTT GGCGGCCCCA 
GCGTTACCAG GAGCATTTAA CTTTGGCGGA GCAACTGCAG CCACGCCAGC TGCCTCAAGT 
GCTCCATTTG TATTCGGAAG CAGCACCAAT GAGCCGCTGG CCAAACCGTC GTTCAACTTC 
ACCGGCTCGG CGGCCAGCTC GACTGCTCCT GCGCCAGCCT TCAATTTCAC GGCCAATACG 
GCGGCCACTA ATAATCCCTC CGGTGGCGAT TCCACACCGA ATGCCAATGC ACTGTTCCAA 
TTTAGCGCGA CCTCCACAGC GCCAGCCAAT ATATTTGCCT TCAATCCGCC AGCTGCGGGC 
AACTCGGCGC AGAGTTCCCA GACGGCTCGC CGCAAAATCC GCGCCCCCGT ACGCCGTTTG 
CCGCCCCGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nup153 ( NM_001144734.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001138206.1 Drosophila melanogaster nucleoporin 153kD, transcript variant C (Nup153), mRNA.

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CloneID	OFa03037
Clone ID Related Accession (Same CDS sequence)	NM_132908.5 , NM_001144734.2
Accession Version	NM_001144734.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nucleoporin 153kD, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001144734.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGATG CACAGGAGCA AAGGGAATCC TCGCAAGCCG AATTGGCCAA AAAGCATCCG 
CTGACGCCGC TCAAGGAGCT GCCTGAAGAA AGCGAGGAGG AGGAGGAGGA AGAGGATGAA 
TCCTCGGCGG GAGCCCTACG GGAGTCTGAC AGCAACAACT CCATCATGGG GAAGATGAAG 
ATGCGCGTGT CCAGCATTCT GCCCGCCTCC TTGAGCGGTT GGTTTTCGCC GTCGTCCAAA 
GATGGGAACG ATGCCTTATC CTCCCCAGCT AATCTCCGCC AGTCACAGCC ACGCCAGTCC 
AATGGACGCC TCACTACCAA GCGGAAACGA GGACGCCGTC GGATAATGCT AGCCGAAGTG 
GATGCCGATG CAGCCGATGA TCTTGATGAC GGCTCCGATG CCAAAGGCCT CAACTATGAG 
GAGGTGGCCC TGGCCGACAA CATAGCCGAA CACGACCTGG CCGCCGAGGA TGAACAGACG 
CGGCGGAGCG AGTACAATGT GTTCCTGCTG CGCAAGCGAG CCGGGGCTGT TGCAGCTGCC 
GGCGGTGACG AGGATGAGGC CGAGGAGGAT GAACTGGAAG AGGACGACGA GGATGGCGAT 
GAGGAGGATG ACGATGAAGA GCAGGAGAAT CTCCAACAGT CCGCCGCCGT GCAGACCAAG 
CGTCGACGCC TGGAGCTGGA GACCCCCGTC AATTTGCCGA ACATGCGTCG TCTGCCCCTT 
TTGTCATCCA CGCCTGCTGC TCCGCTCGCG GCTGCCACCA GCTCCTCCAG CTCCCAGATG 
TACAAGGGCG TCAGCCACAT TGCGCCACAT CGGCGCAATC ACCTCAATTT GTATGGTAGT 
CAACGGCAGC GCGAACCCGC GTACAACTTT TTCACGGGCA ACGAAGCTGC CGAGGGAAGC 
ACCGGGGATC TGCCACACAG CATCCGCCGT TCACTGAACA TACCTTTCGG AGGCAGCAGC 
ACCGCTACCA GCTACAACAA CAGCCTATCC AGCCTTCCTA ACCACAAGCG ACCATCGCTC 
ATAGGCAAGC AAACCCATCG ACGCGATCTT ACCATGGATG AGACGGGGAC TGGCCCGGCC 
ATGAGTTCGG AAGAACATCT CAATCATTTG CTGAGAATAA GCAGGACCAA CAACAATACT 
AGCAACAATA ATAATAATAA TAATAACAAC AACAATGTCA TTGAGACAAA AACAAGACGA 
AGTGAACTGT CTGCGGCCGC AGGAGGTTGC GGTGATTCGC AGTCCGAGAG CGATATGAAC 
GAATACCACG ACAATGGTGA AGGGCACGAC GGACTTCGTC CGTCGCATTA CAACAGCAAC 
AGCAATTTAG AATTTTACGG AAATCTGCAG AGCTCCAAGT CAATATTTAA CAGAAGCAAC 
ACCGCCGCAC AGCAATCGCA TCGGAATTCT ACGTGGTCGC TGAATTCCCT TACCCAACGA 
CGTCGCTTCA ATGCGTCCAT ATACGGCAGT ACGTCGGCAC TGAGCGACAG TCGTCTGCTT 
AGTGGCAGTG CCTCTAACTC AGGCTCTGCA TCTGCCTCTA GCTCGCCCTT TTACCAGGGA 
CGCACCACCT TCGGGGGCAA TTCCGGCAAC AATCGCCTCT TCAGTCGCAG CAATCTTAGC 
TCATCCGCCG CATCCTCAAT GTTGGGACTC AATTCTGCCG GCAGCTCACC GGCGCATCAG 
CTGCATGCTT CCATGACTGG TGGTATTGGA TACGGTATGA AAGCGGTAGA TATGCGACCT 
TCAGACTCCG GCAGTCTTGC GGAGACATCT GTGGGCCAAG GTGGCAGTAA GAAACCCGGT 
ACCGGGCTGT CGAACACCAC GATGCGCATT CTCAACCTAC TTGAAAGCTA CTCCACGCCA 
TTGATCGATG CCAAGCGAAT GGGGAGCAGC ATCAAGGAGC ATCAAAGCAG TCGCCAACAG 
CGCCAGGGTA CGCCAGCCAC CCCGTATCTA CGTTCCACGA GTGCCAGCCG CAATGTTTCG 
GTGCCCAATC ACATCAACGA GTTGGCGGAA CTGCGCTCTA ACAAACTGCT GGTGCCGACG 
ATGCAGCAGC TTCTGGAACG TCGTCGTCTA CACCGCGTTA CACAGAACTC TCGAGATGTG 
GTTCACAGCC AGAACGTTCG TGCTGGTGGG GAAAACAACC AGGAGAAGCC CAAGCCAACT 
GCACCATATG TTGCTCCGAT AGACCAGTCG GCTAACCATA CTCAGCACAC AAACAAGATG 
CGTTCGCGTC TTTCGCACCA GACGCGCAAC AAGGAGACAC GCACGGCGGA AGAGGAAGCA 
CCCCCGCCGC TGGATTTGCC ACAGATCAGC TTTCCGGACA TGGCAAGCGC CCCAAAGTTT 
GATTTGATAA TCAAACCCAC GGTGCCAGTG GTATCAAAAC CATCTACCAC CGACCCAATC 
CAAAGTAGCA AAAGTAGCAA CACCAACCTC TCTACTACAA ACTCCAAGCA GATGCCAAAC 
TTCCTGGCTA ATCCCCAGCC TGCGGCACCA ATTGTTAACT TCGCTGCCAA TGGAAACGTT 
TCTGCAATCA GTAAACCAAG TAAACGCACA TTCACCTTCT CAGAACCCAC ACCTTTGTCG 
AACTTTCAGG AGAACTGCAT CCCCAAGCCG AAGATCAATC GCAAATACAC CTTCTCGGCA 
CCGGCACCAC TGGATGATTT GCGTATAACG AACAAACAGT CGCAGCCAAC CATAAACGGA 
ACGCCGTCAT CCAAGGAGTG GGAGTGTGAT ACGTGTATGG TGCGAAACAA GCCCGAGATC 
AACAAGTGTG TGGCCTGTGA GACGGCCAAG CCGGTAGCAT CTGCGGCTCC TGTCCAAGCA 
CCATTACCAC CATCTACAGC AGCTATTGAC ACACAAAGTT TCGTTGGTTT TGGGGATCGA 
TTCAAGAAAT CGACGACTGC CTGGGAGTGC GATGCCTGCA TGCTGTCCAA TAAGGCTGAA 
GCCAGCAAAT GCATTGCCTG CGAGACTCCA AGGAAAACTG TTGCACCAAA GGTCAACAAC 
TTCTCACCGT TGATTACAAA TGCGAAAAGC AACGAGTGGG AGTGCTCTGT GTGTCTGGTG 
CGCAACAAAG TCGAGGTCAG CAAGTGTGTG GCCTGTGAGT CGGCCAAACC AGGCGCAACT 
ATGGCTCTCC CTGCAACATC TAATATAGCT GTTGCGACTC CCTCTATAAT TACGGATGGC 
TTTGGGGATC GATTCAAGAA ATCAGCGACT GCCTGGGAGT GCGATGCCTG CATGCTGTCC 
AATAAGGCTG AAGCCAGCAA ATGCATTGCC TGCGAAACTC CGCGAAAATC TTCGACTCCA 
ATAGCGAACA GTAGTTACCC GTCGATCAAC AATAATTTGC CAGCCGGATC CGGTTTTGAT 
ATTTCCTTCA CGCGAAAGGC AAACATGTGG GAGTGCCAAA CATGCCTGGT GATGAACAAG 
TCGAGTGATG AGGAATGCAT TGCCTGTCAA ACGCCAAACT CACAGGCGAG AAACAGCAAC 
AGCGAGTCCG CACTGATTTC ATCCATAAGC TCTTCCTCGG CATCGTTCTC CGGATCTTTA 
TCCAGACCCT CATCTAGATC ATCCTCTGGG TCAACCTCAA CCTGTGGGTC AGTTTGTTCG 
GGATCTATCG TCAGCATTAG CTCCACCACC GAAAGCGCCA AAGCATTATC AGCAAAAAAG 
GTGCCCCCCA AGCCGGATGC CGGATTTCAG CAGCTAGTGG CAGCCCAAAA AACATCAACT 
TGGGAATGCG AAGCATGCCT GGCAAAGAAT GATATGTCGC GGAAGACGTG TATCTGCTGT 
GAACAGATGA TGCCGGAAGC ATTTAACCCT GCCGCAACAA CGGCCAACTC CGCAGCCAGT 
TCGGTGCCCA AATTCCGTTT TGGATTTTCT CATGTAAAGG AGGTTGTCAA GCCATCTGTG 
GAAACGACGA CCACACCAGC ACCAACTTCC GCTCAATTTA GCTTCGGTTT CGGCCAAAGC 
AATCAGGGTA AGGATGTGGC GGACAGCAAG AAGACGGAGG CCCCGAAGAC CTTTATGTTT 
GGCGTGAGCA AAGTGGAAGA GCCCAAGACG GTGAGTTTTG GGACAGGCAT CAAGGAAACA 
ACGGCCACAT CGAGCACAGA AGCTACAGCT CCAACTCCAG CTGCAGCAGC ACCAGCACCA 
GTCCAGTTTG TGTTCAAGGC ACCGACTACC GCAACCACGG CCAGTTCACT CACCACCACA 
ATCAGCACCA CCAGCAACGC TCCTGCTTTG GGTGGCTTCA GCTTTGGCGC TCCATCATCG 
TCCTCTACAG TTTCCAGTTC GACGACTAGT ACCAGTGCGA ATCCCGCGGC TGTCAAACCG 
ATGTTCAGCT GGTCCGGAGC TGGCAGTGCC GTGAGCAGCA CCAGTAGCAG CCAGCAGCCA 
GTGGCCAAGG CGCCAACCCT AGGTTTTGGT GTTTCCTCCA GCACCGTAAC AACGACAACA 
ACCTCTACGA AAGTTTTTGC TTTCACACCG GCATCTGGTC TAGATCCGGC AGCAGCCACC 
TCGGCACCAG CTGCAGGTGC CGGCTTCAGT TTTGGAAGTC AGTCAAAGCC AGCAACCACA 
CAGAACACTG GAACCTTCTT CTTTGGCCAG CCAACAGCGG TTGCGCCAGC AACGCCAACC 
AACCCTAGCG TGAGCTCCAT CTTCGGAGCA CCGGCAACAA GTACCACTGC AAGCACCAGC 
GTGAGTGCCA CCACCAGCAC CAGCACCGCC AACGCCATTG CTTCCAGCTT TGCGCCCACC 
TCGACCCCTC AGCTCTTTGG CAATTGGGGT GAAAAGAAAA CGGACTTGAC CACCTTTGGT 
GCTTCTTCCG GTTCGGGAAC GACTACAACG CCTAGCTTCG GCTGGTCGAG CAATGGAGAT 
GCGGCAAAGA GCAACAGCGC TGCGGTCGGC AGTGCGGCGG TGCCTTCGTC TTCGGCCTCC 
ACGATGGCGA CTCCGATATT CGGCAGCAGT TCCATGTTTG GACCATCCAG TAGCAGTAAC 
AACACCACCA GCACCAGCAC CACTAGTCTA CCATTCGGAT CAGCGGCGAC CACTGCCGCA 
ACTACGCCGG CGGGCGGCAA TGCAGCCCTT ACTGGGTTAT TCGGCAATGT GGGCAACTCT 
CTAGCGGGAG TAGGAGCTCC TGTAGCCACA ACACCAGCGG CAACAGCTGC TGCTCCATTG 
ACCAACATCT TTGGCAATCC CACGCCAGTT GCTGCTGCCG CGCCTGTCTT CGGCAGCGGA 
AGCACCATCC CAAGCGCAGG ATTTGGAGCG CCTGCTGCAG CTGCTCCGTT GGCGGCCCCA 
GCGTTACCAG GAGCATTTAA CTTTGGCGGA GCAACTGCAG CCACGCCAGC TGCCTCAAGT 
GCTCCATTTG TATTCGGAAG CAGCACCAAT GAGCCGCTGG CCAAACCGTC GTTCAACTTC 
ACCGGCTCGG CGGCCAGCTC GACTGCTCCT GCGCCAGCCT TCAATTTCAC GGCCAATACG 
GCGGCCACTA ATAATCCCTC CGGTGGCGAT TCCACACCGA ATGCCAATGC ACTGTTCCAA 
TTTAGCGCGA CCTCCACAGC GCCAGCCAAT ATATTTGCCT TCAATCCGCC AGCTGCGGGC 
AACTCGGCGC AGAGTTCCCA GACGGCTCGC CGCAAAATCC GCGCCCCCGT ACGCCGTTTG 
CCGCCCCGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138206.1
CDS	915..6566
Translation	MEDAQEQRESSQAELAKKHPLTPLKELPEESEEEEEEEDESSAGALRESDSNNSIMGKMKMRVSSILPASLSGWFSPSSKDGNDALSSPANLRQSQPRQSNGRLTTKRKRGRRRIMLAEVDADAADDLDDGSDAKGLNYEEVALADNIAEHDLAAEDEQTRRSEYNVFLLRKRAGAVAAAGGDEDEAEEDELEEDDEDGDEEDDDEEQENLQQSAAVQTKRRRLELETPVNLPNMRRLPLLSSTPAAPLAAATSSSSSQMYKGVSHIAPHRRNHLNLYGSQRQREPAYNFFTGNEAAEGSTGDLPHSIRRSLNIPFGGSSTATSYNNSLSSLPNHKRPSLIGKQTHRRDLTMDETGTGPAMSSEEHLNHLLRISRTNNNTSNNNNNNNNNNNVIETKTRRSELSAAAGGCGDSQSESDMNEYHDNGEGHDGLRPSHYNSNSNLEFYGNLQSSKSIFNRSNTAAQQSHRNSTWSLNSLTQRRRFNASIYGSTSALSDSRLLSGSASNSGSASASSSPFYQGRTTFGGNSGNNRLFSRSNLSSSAASSMLGLNSAGSSPAHQLHASMTGGIGYGMKAVDMRPSDSGSLAETSVGQGGSKKPGTGLSNTTMRILNLLESYSTPLIDAKRMGSSIKEHQSSRQQRQGTPATPYLRSTSASRNVSVPNHINELAELRSNKLLVPTMQQLLERRRLHRVTQNSRDVVHSQNVRAGGENNQEKPKPTAPYVAPIDQSANHTQHTNKMRSRLSHQTRNKETRTAEEEAPPPLDLPQISFPDMASAPKFDLIIKPTVPVVSKPSTTDPIQSSKSSNTNLSTTNSKQMPNFLANPQPAAPIVNFAANGNVSAISKPSKRTFTFSEPTPLSNFQENCIPKPKINRKYTFSAPAPLDDLRITNKQSQPTINGTPSSKEWECDTCMVRNKPEINKCVACETAKPVASAAPVQAPLPPSTAAIDTQSFVGFGDRFKKSTTAWECDACMLSNKAEASKCIACETPRKTVAPKVNNFSPLITNAKSNEWECSVCLVRNKVEVSKCVACESAKPGATMALPATSNIAVATPSIITDGFGDRFKKSATAWECDACMLSNKAEASKCIACETPRKSSTPIANSSYPSINNNLPAGSGFDISFTRKANMWECQTCLVMNKSSDEECIACQTPNSQARNSNSESALISSISSSSASFSGSLSRPSSRSSSGSTSTCGSVCSGSIVSISSTTESAKALSAKKVPPKPDAGFQQLVAAQKTSTWECEACLAKNDMSRKTCICCEQMMPEAFNPAATTANSAASSVPKFRFGFSHVKEVVKPSVETTTTPAPTSAQFSFGFGQSNQGKDVADSKKTEAPKTFMFGVSKVEEPKTVSFGTGIKETTATSSTEATAPTPAAAAPAPVQFVFKAPTTATTASSLTTTISTTSNAPALGGFSFGAPSSSSTVSSSTTSTSANPAAVKPMFSWSGAGSAVSSTSSSQQPVAKAPTLGFGVSSSTVTTTTTSTKVFAFTPASGLDPAAATSAPAAGAGFSFGSQSKPATTQNTGTFFFGQPTAVAPATPTNPSVSSIFGAPATSTTASTSVSATTSTSTANAIASSFAPTSTPQLFGNWGEKKTDLTTFGASSGSGTTTTPSFGWSSNGDAAKSNSAAVGSAAVPSSSASTMATPIFGSSSMFGPSSSSNNTTSTSTTSLPFGSAATTAATTPAGGNAALTGLFGNVGNSLAGVGAPVATTPAATAAAPLTNIFGNPTPVAAAAPVFGSGSTIPSAGFGAPAAAAPLAAPALPGAFNFGGATAATPAASSAPFVFGSSTNEPLAKPSFNFTGSAASSTAPAPAFNFTANTAATNNPSGGDSTPNANALFQFSATSTAPANIFAFNPPAAGNSAQSSQTARRKIRAPVRRLPPR

Target ORF information:

RefSeq Version	NM_001144734.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nucleoporin 153kD, transcript variant C (Nup153), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144734.2

ORF Insert Sequence:

ATGGAGGATG CACAGGAGCA AAGGGAATCC TCGCAAGCCG AATTGGCCAA AAAGCATCCG 
CTGACGCCGC TCAAGGAGCT GCCTGAAGAA AGCGAGGAGG AGGAGGAGGA AGAGGATGAA 
TCCTCGGCGG GAGCCCTACG GGAGTCTGAC AGCAACAACT CCATCATGGG GAAGATGAAG 
ATGCGCGTGT CCAGCATTCT GCCCGCCTCC TTGAGCGGTT GGTTTTCGCC GTCGTCCAAA 
GATGGGAACG ATGCCTTATC CTCCCCAGCT AATCTCCGCC AGTCACAGCC ACGCCAGTCC 
AATGGACGCC TCACTACCAA GCGGAAACGA GGACGCCGTC GGATAATGCT AGCCGAAGTG 
GATGCCGATG CAGCCGATGA TCTTGATGAC GGCTCCGATG CCAAAGGCCT CAACTATGAG 
GAGGTGGCCC TGGCCGACAA CATAGCCGAA CACGACCTGG CCGCCGAGGA TGAACAGACG 
CGGCGGAGCG AGTACAATGT GTTCCTGCTG CGCAAGCGAG CCGGGGCTGT TGCAGCTGCC 
GGCGGTGACG AGGATGAGGC CGAGGAGGAT GAACTGGAAG AGGACGACGA GGATGGCGAT 
GAGGAGGATG ACGATGAAGA GCAGGAGAAT CTCCAACAGT CCGCCGCCGT GCAGACCAAG 
CGTCGACGCC TGGAGCTGGA GACCCCCGTC AATTTGCCGA ACATGCGTCG TCTGCCCCTT 
TTGTCATCCA CGCCTGCTGC TCCGCTCGCG GCTGCCACCA GCTCCTCCAG CTCCCAGATG 
TACAAGGGCG TCAGCCACAT TGCGCCACAT CGGCGCAATC ACCTCAATTT GTATGGTAGT 
CAACGGCAGC GCGAACCCGC GTACAACTTT TTCACGGGCA ACGAAGCTGC CGAGGGAAGC 
ACCGGGGATC TGCCACACAG CATCCGCCGT TCACTGAACA TACCTTTCGG AGGCAGCAGC 
ACCGCTACCA GCTACAACAA CAGCCTATCC AGCCTTCCTA ACCACAAGCG ACCATCGCTC 
ATAGGCAAGC AAACCCATCG ACGCGATCTT ACCATGGATG AGACGGGGAC TGGCCCGGCC 
ATGAGTTCGG AAGAACATCT CAATCATTTG CTGAGAATAA GCAGGACCAA CAACAATACT 
AGCAACAATA ATAATAATAA TAATAACAAC AACAATGTCA TTGAGACAAA AACAAGACGA 
AGTGAACTGT CTGCGGCCGC AGGAGGTTGC GGTGATTCGC AGTCCGAGAG CGATATGAAC 
GAATACCACG ACAATGGTGA AGGGCACGAC GGACTTCGTC CGTCGCATTA CAACAGCAAC 
AGCAATTTAG AATTTTACGG AAATCTGCAG AGCTCCAAGT CAATATTTAA CAGAAGCAAC 
ACCGCCGCAC AGCAATCGCA TCGGAATTCT ACGTGGTCGC TGAATTCCCT TACCCAACGA 
CGTCGCTTCA ATGCGTCCAT ATACGGCAGT ACGTCGGCAC TGAGCGACAG TCGTCTGCTT 
AGTGGCAGTG CCTCTAACTC AGGCTCTGCA TCTGCCTCTA GCTCGCCCTT TTACCAGGGA 
CGCACCACCT TCGGGGGCAA TTCCGGCAAC AATCGCCTCT TCAGTCGCAG CAATCTTAGC 
TCATCCGCCG CATCCTCAAT GTTGGGACTC AATTCTGCCG GCAGCTCACC GGCGCATCAG 
CTGCATGCTT CCATGACTGG TGGTATTGGA TACGGTATGA AAGCGGTAGA TATGCGACCT 
TCAGACTCCG GCAGTCTTGC GGAGACATCT GTGGGCCAAG GTGGCAGTAA GAAACCCGGT 
ACCGGGCTGT CGAACACCAC GATGCGCATT CTCAACCTAC TTGAAAGCTA CTCCACGCCA 
TTGATCGATG CCAAGCGAAT GGGGAGCAGC ATCAAGGAGC ATCAAAGCAG TCGCCAACAG 
CGCCAGGGTA CGCCAGCCAC CCCGTATCTA CGTTCCACGA GTGCCAGCCG CAATGTTTCG 
GTGCCCAATC ACATCAACGA GTTGGCGGAA CTGCGCTCTA ACAAACTGCT GGTGCCGACG 
ATGCAGCAGC TTCTGGAACG TCGTCGTCTA CACCGCGTTA CACAGAACTC TCGAGATGTG 
GTTCACAGCC AGAACGTTCG TGCTGGTGGG GAAAACAACC AGGAGAAGCC CAAGCCAACT 
GCACCATATG TTGCTCCGAT AGACCAGTCG GCTAACCATA CTCAGCACAC AAACAAGATG 
CGTTCGCGTC TTTCGCACCA GACGCGCAAC AAGGAGACAC GCACGGCGGA AGAGGAAGCA 
CCCCCGCCGC TGGATTTGCC ACAGATCAGC TTTCCGGACA TGGCAAGCGC CCCAAAGTTT 
GATTTGATAA TCAAACCCAC GGTGCCAGTG GTATCAAAAC CATCTACCAC CGACCCAATC 
CAAAGTAGCA AAAGTAGCAA CACCAACCTC TCTACTACAA ACTCCAAGCA GATGCCAAAC 
TTCCTGGCTA ATCCCCAGCC TGCGGCACCA ATTGTTAACT TCGCTGCCAA TGGAAACGTT 
TCTGCAATCA GTAAACCAAG TAAACGCACA TTCACCTTCT CAGAACCCAC ACCTTTGTCG 
AACTTTCAGG AGAACTGCAT CCCCAAGCCG AAGATCAATC GCAAATACAC CTTCTCGGCA 
CCGGCACCAC TGGATGATTT GCGTATAACG AACAAACAGT CGCAGCCAAC CATAAACGGA 
ACGCCGTCAT CCAAGGAGTG GGAGTGTGAT ACGTGTATGG TGCGAAACAA GCCCGAGATC 
AACAAGTGTG TGGCCTGTGA GACGGCCAAG CCGGTAGCAT CTGCGGCTCC TGTCCAAGCA 
CCATTACCAC CATCTACAGC AGCTATTGAC ACACAAAGTT TCGTTGGTTT TGGGGATCGA 
TTCAAGAAAT CGACGACTGC CTGGGAGTGC GATGCCTGCA TGCTGTCCAA TAAGGCTGAA 
GCCAGCAAAT GCATTGCCTG CGAGACTCCA AGGAAAACTG TTGCACCAAA GGTCAACAAC 
TTCTCACCGT TGATTACAAA TGCGAAAAGC AACGAGTGGG AGTGCTCTGT GTGTCTGGTG 
CGCAACAAAG TCGAGGTCAG CAAGTGTGTG GCCTGTGAGT CGGCCAAACC AGGCGCAACT 
ATGGCTCTCC CTGCAACATC TAATATAGCT GTTGCGACTC CCTCTATAAT TACGGATGGC 
TTTGGGGATC GATTCAAGAA ATCAGCGACT GCCTGGGAGT GCGATGCCTG CATGCTGTCC 
AATAAGGCTG AAGCCAGCAA ATGCATTGCC TGCGAAACTC CGCGAAAATC TTCGACTCCA 
ATAGCGAACA GTAGTTACCC GTCGATCAAC AATAATTTGC CAGCCGGATC CGGTTTTGAT 
ATTTCCTTCA CGCGAAAGGC AAACATGTGG GAGTGCCAAA CATGCCTGGT GATGAACAAG 
TCGAGTGATG AGGAATGCAT TGCCTGTCAA ACGCCAAACT CACAGGCGAG AAACAGCAAC 
AGCGAGTCCG CACTGATTTC ATCCATAAGC TCTTCCTCGG CATCGTTCTC CGGATCTTTA 
TCCAGACCCT CATCTAGATC ATCCTCTGGG TCAACCTCAA CCTGTGGGTC AGTTTGTTCG 
GGATCTATCG TCAGCATTAG CTCCACCACC GAAAGCGCCA AAGCATTATC AGCAAAAAAG 
GTGCCCCCCA AGCCGGATGC CGGATTTCAG CAGCTAGTGG CAGCCCAAAA AACATCAACT 
TGGGAATGCG AAGCATGCCT GGCAAAGAAT GATATGTCGC GGAAGACGTG TATCTGCTGT 
GAACAGATGA TGCCGGAAGC ATTTAACCCT GCCGCAACAA CGGCCAACTC CGCAGCCAGT 
TCGGTGCCCA AATTCCGTTT TGGATTTTCT CATGTAAAGG AGGTTGTCAA GCCATCTGTG 
GAAACGACGA CCACACCAGC ACCAACTTCC GCTCAATTTA GCTTCGGTTT CGGCCAAAGC 
AATCAGGGTA AGGATGTGGC GGACAGCAAG AAGACGGAGG CCCCGAAGAC CTTTATGTTT 
GGCGTGAGCA AAGTGGAAGA GCCCAAGACG GTGAGTTTTG GGACAGGCAT CAAGGAAACA 
ACGGCCACAT CGAGCACAGA AGCTACAGCT CCAACTCCAG CTGCAGCAGC ACCAGCACCA 
GTCCAGTTTG TGTTCAAGGC ACCGACTACC GCAACCACGG CCAGTTCACT CACCACCACA 
ATCAGCACCA CCAGCAACGC TCCTGCTTTG GGTGGCTTCA GCTTTGGCGC TCCATCATCG 
TCCTCTACAG TTTCCAGTTC GACGACTAGT ACCAGTGCGA ATCCCGCGGC TGTCAAACCG 
ATGTTCAGCT GGTCCGGAGC TGGCAGTGCC GTGAGCAGCA CCAGTAGCAG CCAGCAGCCA 
GTGGCCAAGG CGCCAACCCT AGGTTTTGGT GTTTCCTCCA GCACCGTAAC AACGACAACA 
ACCTCTACGA AAGTTTTTGC TTTCACACCG GCATCTGGTC TAGATCCGGC AGCAGCCACC 
TCGGCACCAG CTGCAGGTGC CGGCTTCAGT TTTGGAAGTC AGTCAAAGCC AGCAACCACA 
CAGAACACTG GAACCTTCTT CTTTGGCCAG CCAACAGCGG TTGCGCCAGC AACGCCAACC 
AACCCTAGCG TGAGCTCCAT CTTCGGAGCA CCGGCAACAA GTACCACTGC AAGCACCAGC 
GTGAGTGCCA CCACCAGCAC CAGCACCGCC AACGCCATTG CTTCCAGCTT TGCGCCCACC 
TCGACCCCTC AGCTCTTTGG CAATTGGGGT GAAAAGAAAA CGGACTTGAC CACCTTTGGT 
GCTTCTTCCG GTTCGGGAAC GACTACAACG CCTAGCTTCG GCTGGTCGAG CAATGGAGAT 
GCGGCAAAGA GCAACAGCGC TGCGGTCGGC AGTGCGGCGG TGCCTTCGTC TTCGGCCTCC 
ACGATGGCGA CTCCGATATT CGGCAGCAGT TCCATGTTTG GACCATCCAG TAGCAGTAAC 
AACACCACCA GCACCAGCAC CACTAGTCTA CCATTCGGAT CAGCGGCGAC CACTGCCGCA 
ACTACGCCGG CGGGCGGCAA TGCAGCCCTT ACTGGGTTAT TCGGCAATGT GGGCAACTCT 
CTAGCGGGAG TAGGAGCTCC TGTAGCCACA ACACCAGCGG CAACAGCTGC TGCTCCATTG 
ACCAACATCT TTGGCAATCC CACGCCAGTT GCTGCTGCCG CGCCTGTCTT CGGCAGCGGA 
AGCACCATCC CAAGCGCAGG ATTTGGAGCG CCTGCTGCAG CTGCTCCGTT GGCGGCCCCA 
GCGTTACCAG GAGCATTTAA CTTTGGCGGA GCAACTGCAG CCACGCCAGC TGCCTCAAGT 
GCTCCATTTG TATTCGGAAG CAGCACCAAT GAGCCGCTGG CCAAACCGTC GTTCAACTTC 
ACCGGCTCGG CGGCCAGCTC GACTGCTCCT GCGCCAGCCT TCAATTTCAC GGCCAATACG 
GCGGCCACTA ATAATCCCTC CGGTGGCGAT TCCACACCGA ATGCCAATGC ACTGTTCCAA 
TTTAGCGCGA CCTCCACAGC GCCAGCCAAT ATATTTGCCT TCAATCCGCC AGCTGCGGGC 
AACTCGGCGC AGAGTTCCCA GACGGCTCGC CGCAAAATCC GCGCCCCCGT ACGCCGTTTG 
CCGCCCCGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nup153 ( NM_001103532.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097002.1 Drosophila melanogaster nucleoporin 153kD, transcript variant D (Nup153), mRNA.

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CloneID	OFa03038
Clone ID Related Accession (Same CDS sequence)	NM_001103532.3
Accession Version	NM_001103532.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5790bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nucleoporin 153kD, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001103532.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGATG CACAGGAGCA AAGGGAATCC TCGCAAGCCG AATTGGCCAA AAAGCATCCG 
CTGACGCCGC TCAAGGAGCT GCCTGAAGAA AGCGAGGAGG AGGAGGAGGA AGAGGATGAA 
TCCTCGGCGG GAGCCCTACG GGAGTCTGAC AGCAACAACT CCATCATGGG GAAGATGAAG 
ATGCGCGTGT CCAGCATTCT GCCCGCCTCC TTGAGCGGTT GGTTTTCGCC GTCGTCCAAA 
GATGGGAACG ATGCCTTATC CTCCCCAGCT AATCTCCGCC AGTCACAGCC ACGCCAGTCC 
AATGGACGCC TCACTACCAA GCGGAAACGA GGACGCCGTC GGATAATGCT AGCCGAAGTG 
GATGCCGATG CAGCCGATGA TCTTGATGAC GGCTCCGATG CCAAAGGCCT CAACTATGAG 
GAGGTGGCCC TGGCCGACAA CATAGCCGAA CACGACCTGG CCGCCGAGGA TGAACAGACG 
CGGCGGAGCG AGTACAATGT GTTCCTGCTG CGCAAGCGAG CCGGGGCTGT TGCAGCTGCC 
GGCGGTGACG AGGATGAGGC CGAGGAGGAT GAACTGGAAG AGGACGACGA GGATGGCGAT 
GAGGAGGATG ACGATGAAGA GCAGGAGAAT CTCCAACAGT CCGCCGCCGT GCAGACCAAG 
CGTCGACGCC TGGAGCTGGA GACCCCCGTC AATTTGCCGA ACATGCGTCG TCTGCCCCTT 
TTGTCATCCA CGCCTGCTGC TCCGCTCGCG GCTGCCACCA GCTCCTCCAG CTCCCAGATG 
TACAAGGGCG TCAGCCACAT TGCGCCACAT CGGCGCAATC ACCTCAATTT GTATGGTAGT 
CAACGGCAGC GCGAACCCGC GTACAACTTT TTCACGGGCA ACGAAGCTGC CGAGGGAAGC 
ACCGGGGATC TGCCACACAG CATCCGCCGT TCACTGAACA TACCTTTCGG AGGCAGCAGC 
ACCGCTACCA GCTACAACAA CAGCCTATCC AGCCTTCCTA ACCACAAGCG ACCATCGCTC 
ATAGGCAAGC AAACCCATCG ACGCGATCTT ACCATGGATG AGACGGGGAC TGGCCCGGCC 
ATGAGTTCGG AAGAACATCT CAATCATTTG CTGAGAATAA GCAGGACCAA CAACAATACT 
AGCAACAATA ATAATAATAA TAATAACAAC AACAATGTCA TTGAGACAAA AACAAGACGA 
AGTGAACTGT CTGCGGCCGC AGGAGGTTGC GGTGATTCGC AGTCCGAGAG CGATATGAAC 
GAATACCACG ACAATGGTGA AGGGCACGAC GGACTTCGTC CGTCGCATTA CAACAGCAAC 
AGCAATTTAG AATTTTACGG AAATCTGCAG AGCTCCAAGT CAATATTTAA CAGAAGCAAC 
ACCGCCGCAC AGCAATCGCA TCGGAATTCT ACGTGGTCGC TGAATTCCCT TACCCAACGA 
CGTCGCTTCA ATGCGTCCAT ATACGGCAGT ACGTCGGCAC TGAGCGACAG TCGTCTGCTT 
AGTGGCAGTG CCTCTAACTC AGGCTCTGCA TCTGCCTCTA GCTCGCCCTT TTACCAGGGA 
CGCACCACCT TCGGGGGCAA TTCCGGCAAC AATCGCCTCT TCAGTCGCAG CAATCTTAGC 
TCATCCGCCG CATCCTCAAT GTTGGGACTC AATTCTGCCG GCAGCTCACC GGCGCATCAG 
CTGCATGCTT CCATGACTGG TGGTATTGGA TACGGTATGA AAGCGGTAGA TATGCGACCT 
TCAGACTCCG GCAGTCTTGC GGAGACATCT GTGGGCCAAG GTGGCAGTAA GAAACCCGGT 
ACCGGGCTGT CGAACACCAC GATGCGCATT CTCAACCTAC TTGAAAGCTA CTCCACGCCA 
TTGATCGATG CCAAGCGAAT GGGGAGCAGC ATCAAGGAGC ATCAAAGCAG TCGCCAACAG 
CGCCAGGGTA CGCCAGCCAC CCCGTATCTA CGTTCCACGA GTGCCAGCCG CAATGTTTCG 
GTGCCCAATC ACATCAACGA GTTGGCGGAA CTGCGCTCTA ACAAACTGCT GGTGCCGACG 
ATGCAGCAGC TTCTGGAACG TCGTCGTCTA CACCGCGTTA CACAGAACTC TCGAGATGTG 
GTTCACAGCC AGAACGTTCG TGCTGGTGGG GAAAACAACC AGGAGAAGCC CAAGCCAACT 
GCACCATATG TTGCTCCGAT AGACCAGTCG GCTAACCATA CTCAGCACAC AAACAAGATG 
CGTTCGCGTC TTTCGCACCA GACGCGCAAC AAGGAGACAC GCACGGCGGA AGAGGAAGCA 
CCCCCGCCGC TGGATTTGCC ACAGATCAGC TTTCCGGACA TGGCAAGCGC CCCAAAGTTT 
GATTTGATAA TCAAACCCAC GGTGCCAGTG GTATCAAAAC CATCTACCAC CGACCCAATC 
CAAAGTAGCA AAAGTAGCAA CACCAACCTC TCTACTACAA ACTCCAAGCA GATGCCAAAC 
TTCCTGGCTA ATCCCCAGCC TGCGGCACCA ATTGTTAACT TCGCTGCCAA TGGAAACGTT 
TCTGCAATCA GTAAACCAAG TAAACGCACA TTCACCTTCT CAGAACCCAC ACCTTTGTCG 
AACTTTCAGG AGAACTGCAT CCCCAAGCCG AAGATCAATC GCAAATACAC CTTCTCGGCA 
CCGGCACCAC TGGATGATTT GCGTATAACG AACAAACAGT CGCAGCCAAC CATAAACGGA 
ACGCCGTCAT CCAAGGAGTG GGAGTGTGAT ACGTGTATGG TGCGAAACAA GCCCGAGATC 
AACAAGTGTG TGGCCTGTGA GACGGCCAAG CCGGTAGCAT CTGCGGCTCC TGTCCAAGCA 
CCATTACCAC CATCTACAGC AGCTATTGAC ACACAAAGTT TCGTTGGTTT TGGGGATCGA 
TTCAAGAAAT CGACGACTGC CTGGGAGTGC GATGCCTGCA TGCTGTCCAA TAAGGCTGAA 
GCCAGCAAAT GCATTGCCTG CGAGACTCCA AGGAAAACTG TTGCACCAAA GGTCAACAAC 
TTCTCACCGT TGATTACAAA TGCGAAAAGC AACGAGTGGG AGTGCTCTGT GTGTCTGGTG 
CGCAACAAAG TCGAGGTCAG CAAGTGTGTG GCCTGTGAGT CGGCCAAACC AGGCGCAACT 
ATGGCTCTCC CTGCAACATC TAATATAGCT GTTGCGACTC CCTCTATAAT TACGGATGGC 
TTTGGGGATC GATTCAAGAA ATCAGCGACT GCCTGGGAGT GCGATGCCTG CATGCTGTCC 
AATAAGGCTG AAGCCAGCAA ATGCATTGCC TGCGAAACTC CGCGAAAATC TTCGACTCCA 
ATAGCGAACA GTAGTTACCC GTCGATCAAC AATAATTTGC CAGCCGGATC CGGTTTTGAT 
ATTTCCTTCA CGCGAAAGGC AAACATGTGG GAGTGCCAAA CATGCCTGGT GATGAACAAG 
TCGAGTGATG AGGAATGCAT TGCCTGTCAA ACGCCAAACT CACAGGCGAG AAACAGCAAC 
AGCGAGTCCG CACTGATTTC ATCCATAAGC TCTTCCTCGG CATCGTTCTC CGGATCTTTA 
TCCAGACCCT CATCTAGATC ATCCTCTGGG TCAACCTCAA CCTGTGGGTC AGTTTGTTCG 
GGATCTATCG TCAGCATTAG CTCCACCACC GAAAGCGCCA AAGCATTATC AGCAAAAAAG 
GTGCCCCCCA AGCCGGATGC CGGATTTCAG CAGCTAGTGG CAGCCCAAAA AACATCAACT 
TGGGAATGCG AAGCATGCCT GGCAAAGAAT GATATGTCGC GGAAGACGTG TATCTGCTGT 
GAACAGATGA TGCCGGAAGC ATTTAACCCT GCCGCAACAA CGGCCAACTC CGCAGCCAGT 
TCGGTGCCCA AATTCCGTTT TGGATTTTCT CATGTAAAGG AGGTTGTCAA GCCATCTGTG 
GAAACGACGA CCACACCAGC ACCAACTTCC GCTCAATTTA GCTTCGGTTT CGGCCAAAGC 
AATCAGGGTA AGGATGTGGC GGACAGCAAG AAGACGGAGG CCCCGAAGAC CTTTATGTTT 
GGCGTGAGCA AAGTGGAAGA GCCCAAGACG GTGAGTTTTG GGACAGGCAT CAAGGAAACA 
ACGGCCACAT CGAGCACAGA AGCTACAGCT CCAACTCCAG CTGCAGCAGC ACCAGCACCA 
GTCCAGTTTG TGTTCAAGGC ACCGACTACC GCAACCACGG CCAGTTCACT CACCACCACA 
ATCAGCACCA CCAGCAACGC TCCTGCTTTG GGTGGCTTCA GCTTTGGCGC TCCATCATCG 
TCCTCTACAG TTTCCAGTTC GACGACTAGT ACCAGTGCGA ATCCCGCGGC TGTCAAACCG 
ATGTTCAGCT GGTCCGGAGC TGGCAGTGCC GTGAGCAGCA CCAGTAGCAG CCAGCAGCCA 
GTGGCCAAGG CGCCAACCCT AGGTTTTGGT GTTTCCTCCA GCACCGTAAC AACGACAACA 
ACCTCTACGA AAGTTTTTGC TTTCACACCG GCATCTGGTC TAGATCCGGC AGCAGCCACC 
TCGGCACCAG CTGCAGGTGC CGGCTTCAGT TTTGGAAGTC AGTCAAAGCC AGCAACCACA 
CAGAACACTG GAACCTTCTT CTTTGGCCAG CCAACAGCGG TTGCGCCAGC AACGCCAACC 
AACCCTAGCG TGAGCTCCAT CTTCGGAGCA CCGGCAACAA GTACCACTGC AAGCACCAGC 
GTGAGTGCCA CCACCAGCAC CAGCACCGCC AACGCCATTG CTTCCAGCTT TGCGCCCACC 
TCGACCCCTC AGCTCTTTGG CAATTGGGGT GAAAAGAAAA CGGACTTGAC CACCTTTGGT 
GCTTCTTCCG GTTCGGGAAC GACTACAACG CCTAGCTTCG GCTGGTCGAG CAATGGAGAT 
GCGGCAAAGA GCAACAGCGC TGCGGTCGGC AGTGCGGCGG TGCCTTCGTC TTCGGCCTCC 
ACGATGGCGA CTCCGATATT CGGCAGCAGT TCCATGTTTG GACCATCCAG TAGCAGTAAC 
AACACCACCA GCACCAGCAC CACTAGTCTA CCATTCGGAT CAGCGGCGAC CACTGCCGCA 
ACTACGCCGG CGGGCGGCAA TGCAGCCCTT ACTGGGTTAT TCGGCAATGT GGGCAACTCT 
CTAGCGGGAG TAGGAGCTCC TGTAGCCACA ACACCAGCGG CAACAGCTGC TGCTCCATTG 
ACCAACATCT TTGGCAATCC CACGCCAGTT GCTGCTGCCG CGCCTGTCTT CGGCAGCGGA 
AGCACCATCC CAAGCGCAGG ATTTGGAGCG CCTGCTGCAG CTGCTCCGTT GGCGGCCCCA 
GCGTTACCAG GAGCATTTAA CTTTGGCGGA GCAACTGCAG CCACGCCAGC TGCCTCAAGT 
GCTCCATTTG TATTCGGAAG CAGCACCAAT GAGCCGCTGG CCAAACCGTC GTTCAACTTC 
ACCGGCTCGG CGGCCAGCTC GACTGCTCCT GCGCCAGCCT TCAATTTCAC GGCCAATACG 
GCGGCCACTA ATAATCCCTC CGGTGGCGAT TCGCATCCGC GCATCTTCCA ATTCGGTAGT 
TCGCAACCGG CGGCCAATAA CCAGTTTGGT GGTGCGGGCG GTGCCACCAA CAACTCCGCC 
GGCGGTATGC CCATGTTCAA CTTTTCGCCC GGGGCGCCGC AGATGCAGTC CACACCGAAT 
GCCAATGCAC TGTTCCAATT TAGCGCGACC TCCACAGCGC CAGCCAATAT ATTTGCCTTC 
AATCCGCCAG CTGCGGGCAA CTCGGCGCAG AGTTCCCAGA CGGCTCGCCG CAAAATCCGC 
GCCCCCGTAC GCCGTTTGCC GCCCCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097002.1
CDS	831..6620
Translation	MEDAQEQRESSQAELAKKHPLTPLKELPEESEEEEEEEDESSAGALRESDSNNSIMGKMKMRVSSILPASLSGWFSPSSKDGNDALSSPANLRQSQPRQSNGRLTTKRKRGRRRIMLAEVDADAADDLDDGSDAKGLNYEEVALADNIAEHDLAAEDEQTRRSEYNVFLLRKRAGAVAAAGGDEDEAEEDELEEDDEDGDEEDDDEEQENLQQSAAVQTKRRRLELETPVNLPNMRRLPLLSSTPAAPLAAATSSSSSQMYKGVSHIAPHRRNHLNLYGSQRQREPAYNFFTGNEAAEGSTGDLPHSIRRSLNIPFGGSSTATSYNNSLSSLPNHKRPSLIGKQTHRRDLTMDETGTGPAMSSEEHLNHLLRISRTNNNTSNNNNNNNNNNNVIETKTRRSELSAAAGGCGDSQSESDMNEYHDNGEGHDGLRPSHYNSNSNLEFYGNLQSSKSIFNRSNTAAQQSHRNSTWSLNSLTQRRRFNASIYGSTSALSDSRLLSGSASNSGSASASSSPFYQGRTTFGGNSGNNRLFSRSNLSSSAASSMLGLNSAGSSPAHQLHASMTGGIGYGMKAVDMRPSDSGSLAETSVGQGGSKKPGTGLSNTTMRILNLLESYSTPLIDAKRMGSSIKEHQSSRQQRQGTPATPYLRSTSASRNVSVPNHINELAELRSNKLLVPTMQQLLERRRLHRVTQNSRDVVHSQNVRAGGENNQEKPKPTAPYVAPIDQSANHTQHTNKMRSRLSHQTRNKETRTAEEEAPPPLDLPQISFPDMASAPKFDLIIKPTVPVVSKPSTTDPIQSSKSSNTNLSTTNSKQMPNFLANPQPAAPIVNFAANGNVSAISKPSKRTFTFSEPTPLSNFQENCIPKPKINRKYTFSAPAPLDDLRITNKQSQPTINGTPSSKEWECDTCMVRNKPEINKCVACETAKPVASAAPVQAPLPPSTAAIDTQSFVGFGDRFKKSTTAWECDACMLSNKAEASKCIACETPRKTVAPKVNNFSPLITNAKSNEWECSVCLVRNKVEVSKCVACESAKPGATMALPATSNIAVATPSIITDGFGDRFKKSATAWECDACMLSNKAEASKCIACETPRKSSTPIANSSYPSINNNLPAGSGFDISFTRKANMWECQTCLVMNKSSDEECIACQTPNSQARNSNSESALISSISSSSASFSGSLSRPSSRSSSGSTSTCGSVCSGSIVSISSTTESAKALSAKKVPPKPDAGFQQLVAAQKTSTWECEACLAKNDMSRKTCICCEQMMPEAFNPAATTANSAASSVPKFRFGFSHVKEVVKPSVETTTTPAPTSAQFSFGFGQSNQGKDVADSKKTEAPKTFMFGVSKVEEPKTVSFGTGIKETTATSSTEATAPTPAAAAPAPVQFVFKAPTTATTASSLTTTISTTSNAPALGGFSFGAPSSSSTVSSSTTSTSANPAAVKPMFSWSGAGSAVSSTSSSQQPVAKAPTLGFGVSSSTVTTTTTSTKVFAFTPASGLDPAAATSAPAAGAGFSFGSQSKPATTQNTGTFFFGQPTAVAPATPTNPSVSSIFGAPATSTTASTSVSATTSTSTANAIASSFAPTSTPQLFGNWGEKKTDLTTFGASSGSGTTTTPSFGWSSNGDAAKSNSAAVGSAAVPSSSASTMATPIFGSSSMFGPSSSSNNTTSTSTTSLPFGSAATTAATTPAGGNAALTGLFGNVGNSLAGVGAPVATTPAATAAAPLTNIFGNPTPVAAAAPVFGSGSTIPSAGFGAPAAAAPLAAPALPGAFNFGGATAATPAASSAPFVFGSSTNEPLAKPSFNFTGSAASSTAPAPAFNFTANTAATNNPSGGDSHPRIFQFGSSQPAANNQFGGAGGATNNSAGGMPMFNFSPGAPQMQSTPNANALFQFSATSTAPANIFAFNPPAAGNSAQSSQTARRKIRAPVRRLPPR

Target ORF information:

RefSeq Version	NM_001103532.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nucleoporin 153kD, transcript variant D (Nup153), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103532.3

ORF Insert Sequence:

ATGGAGGATG CACAGGAGCA AAGGGAATCC TCGCAAGCCG AATTGGCCAA AAAGCATCCG 
CTGACGCCGC TCAAGGAGCT GCCTGAAGAA AGCGAGGAGG AGGAGGAGGA AGAGGATGAA 
TCCTCGGCGG GAGCCCTACG GGAGTCTGAC AGCAACAACT CCATCATGGG GAAGATGAAG 
ATGCGCGTGT CCAGCATTCT GCCCGCCTCC TTGAGCGGTT GGTTTTCGCC GTCGTCCAAA 
GATGGGAACG ATGCCTTATC CTCCCCAGCT AATCTCCGCC AGTCACAGCC ACGCCAGTCC 
AATGGACGCC TCACTACCAA GCGGAAACGA GGACGCCGTC GGATAATGCT AGCCGAAGTG 
GATGCCGATG CAGCCGATGA TCTTGATGAC GGCTCCGATG CCAAAGGCCT CAACTATGAG 
GAGGTGGCCC TGGCCGACAA CATAGCCGAA CACGACCTGG CCGCCGAGGA TGAACAGACG 
CGGCGGAGCG AGTACAATGT GTTCCTGCTG CGCAAGCGAG CCGGGGCTGT TGCAGCTGCC 
GGCGGTGACG AGGATGAGGC CGAGGAGGAT GAACTGGAAG AGGACGACGA GGATGGCGAT 
GAGGAGGATG ACGATGAAGA GCAGGAGAAT CTCCAACAGT CCGCCGCCGT GCAGACCAAG 
CGTCGACGCC TGGAGCTGGA GACCCCCGTC AATTTGCCGA ACATGCGTCG TCTGCCCCTT 
TTGTCATCCA CGCCTGCTGC TCCGCTCGCG GCTGCCACCA GCTCCTCCAG CTCCCAGATG 
TACAAGGGCG TCAGCCACAT TGCGCCACAT CGGCGCAATC ACCTCAATTT GTATGGTAGT 
CAACGGCAGC GCGAACCCGC GTACAACTTT TTCACGGGCA ACGAAGCTGC CGAGGGAAGC 
ACCGGGGATC TGCCACACAG CATCCGCCGT TCACTGAACA TACCTTTCGG AGGCAGCAGC 
ACCGCTACCA GCTACAACAA CAGCCTATCC AGCCTTCCTA ACCACAAGCG ACCATCGCTC 
ATAGGCAAGC AAACCCATCG ACGCGATCTT ACCATGGATG AGACGGGGAC TGGCCCGGCC 
ATGAGTTCGG AAGAACATCT CAATCATTTG CTGAGAATAA GCAGGACCAA CAACAATACT 
AGCAACAATA ATAATAATAA TAATAACAAC AACAATGTCA TTGAGACAAA AACAAGACGA 
AGTGAACTGT CTGCGGCCGC AGGAGGTTGC GGTGATTCGC AGTCCGAGAG CGATATGAAC 
GAATACCACG ACAATGGTGA AGGGCACGAC GGACTTCGTC CGTCGCATTA CAACAGCAAC 
AGCAATTTAG AATTTTACGG AAATCTGCAG AGCTCCAAGT CAATATTTAA CAGAAGCAAC 
ACCGCCGCAC AGCAATCGCA TCGGAATTCT ACGTGGTCGC TGAATTCCCT TACCCAACGA 
CGTCGCTTCA ATGCGTCCAT ATACGGCAGT ACGTCGGCAC TGAGCGACAG TCGTCTGCTT 
AGTGGCAGTG CCTCTAACTC AGGCTCTGCA TCTGCCTCTA GCTCGCCCTT TTACCAGGGA 
CGCACCACCT TCGGGGGCAA TTCCGGCAAC AATCGCCTCT TCAGTCGCAG CAATCTTAGC 
TCATCCGCCG CATCCTCAAT GTTGGGACTC AATTCTGCCG GCAGCTCACC GGCGCATCAG 
CTGCATGCTT CCATGACTGG TGGTATTGGA TACGGTATGA AAGCGGTAGA TATGCGACCT 
TCAGACTCCG GCAGTCTTGC GGAGACATCT GTGGGCCAAG GTGGCAGTAA GAAACCCGGT 
ACCGGGCTGT CGAACACCAC GATGCGCATT CTCAACCTAC TTGAAAGCTA CTCCACGCCA 
TTGATCGATG CCAAGCGAAT GGGGAGCAGC ATCAAGGAGC ATCAAAGCAG TCGCCAACAG 
CGCCAGGGTA CGCCAGCCAC CCCGTATCTA CGTTCCACGA GTGCCAGCCG CAATGTTTCG 
GTGCCCAATC ACATCAACGA GTTGGCGGAA CTGCGCTCTA ACAAACTGCT GGTGCCGACG 
ATGCAGCAGC TTCTGGAACG TCGTCGTCTA CACCGCGTTA CACAGAACTC TCGAGATGTG 
GTTCACAGCC AGAACGTTCG TGCTGGTGGG GAAAACAACC AGGAGAAGCC CAAGCCAACT 
GCACCATATG TTGCTCCGAT AGACCAGTCG GCTAACCATA CTCAGCACAC AAACAAGATG 
CGTTCGCGTC TTTCGCACCA GACGCGCAAC AAGGAGACAC GCACGGCGGA AGAGGAAGCA 
CCCCCGCCGC TGGATTTGCC ACAGATCAGC TTTCCGGACA TGGCAAGCGC CCCAAAGTTT 
GATTTGATAA TCAAACCCAC GGTGCCAGTG GTATCAAAAC CATCTACCAC CGACCCAATC 
CAAAGTAGCA AAAGTAGCAA CACCAACCTC TCTACTACAA ACTCCAAGCA GATGCCAAAC 
TTCCTGGCTA ATCCCCAGCC TGCGGCACCA ATTGTTAACT TCGCTGCCAA TGGAAACGTT 
TCTGCAATCA GTAAACCAAG TAAACGCACA TTCACCTTCT CAGAACCCAC ACCTTTGTCG 
AACTTTCAGG AGAACTGCAT CCCCAAGCCG AAGATCAATC GCAAATACAC CTTCTCGGCA 
CCGGCACCAC TGGATGATTT GCGTATAACG AACAAACAGT CGCAGCCAAC CATAAACGGA 
ACGCCGTCAT CCAAGGAGTG GGAGTGTGAT ACGTGTATGG TGCGAAACAA GCCCGAGATC 
AACAAGTGTG TGGCCTGTGA GACGGCCAAG CCGGTAGCAT CTGCGGCTCC TGTCCAAGCA 
CCATTACCAC CATCTACAGC AGCTATTGAC ACACAAAGTT TCGTTGGTTT TGGGGATCGA 
TTCAAGAAAT CGACGACTGC CTGGGAGTGC GATGCCTGCA TGCTGTCCAA TAAGGCTGAA 
GCCAGCAAAT GCATTGCCTG CGAGACTCCA AGGAAAACTG TTGCACCAAA GGTCAACAAC 
TTCTCACCGT TGATTACAAA TGCGAAAAGC AACGAGTGGG AGTGCTCTGT GTGTCTGGTG 
CGCAACAAAG TCGAGGTCAG CAAGTGTGTG GCCTGTGAGT CGGCCAAACC AGGCGCAACT 
ATGGCTCTCC CTGCAACATC TAATATAGCT GTTGCGACTC CCTCTATAAT TACGGATGGC 
TTTGGGGATC GATTCAAGAA ATCAGCGACT GCCTGGGAGT GCGATGCCTG CATGCTGTCC 
AATAAGGCTG AAGCCAGCAA ATGCATTGCC TGCGAAACTC CGCGAAAATC TTCGACTCCA 
ATAGCGAACA GTAGTTACCC GTCGATCAAC AATAATTTGC CAGCCGGATC CGGTTTTGAT 
ATTTCCTTCA CGCGAAAGGC AAACATGTGG GAGTGCCAAA CATGCCTGGT GATGAACAAG 
TCGAGTGATG AGGAATGCAT TGCCTGTCAA ACGCCAAACT CACAGGCGAG AAACAGCAAC 
AGCGAGTCCG CACTGATTTC ATCCATAAGC TCTTCCTCGG CATCGTTCTC CGGATCTTTA 
TCCAGACCCT CATCTAGATC ATCCTCTGGG TCAACCTCAA CCTGTGGGTC AGTTTGTTCG 
GGATCTATCG TCAGCATTAG CTCCACCACC GAAAGCGCCA AAGCATTATC AGCAAAAAAG 
GTGCCCCCCA AGCCGGATGC CGGATTTCAG CAGCTAGTGG CAGCCCAAAA AACATCAACT 
TGGGAATGCG AAGCATGCCT GGCAAAGAAT GATATGTCGC GGAAGACGTG TATCTGCTGT 
GAACAGATGA TGCCGGAAGC ATTTAACCCT GCCGCAACAA CGGCCAACTC CGCAGCCAGT 
TCGGTGCCCA AATTCCGTTT TGGATTTTCT CATGTAAAGG AGGTTGTCAA GCCATCTGTG 
GAAACGACGA CCACACCAGC ACCAACTTCC GCTCAATTTA GCTTCGGTTT CGGCCAAAGC 
AATCAGGGTA AGGATGTGGC GGACAGCAAG AAGACGGAGG CCCCGAAGAC CTTTATGTTT 
GGCGTGAGCA AAGTGGAAGA GCCCAAGACG GTGAGTTTTG GGACAGGCAT CAAGGAAACA 
ACGGCCACAT CGAGCACAGA AGCTACAGCT CCAACTCCAG CTGCAGCAGC ACCAGCACCA 
GTCCAGTTTG TGTTCAAGGC ACCGACTACC GCAACCACGG CCAGTTCACT CACCACCACA 
ATCAGCACCA CCAGCAACGC TCCTGCTTTG GGTGGCTTCA GCTTTGGCGC TCCATCATCG 
TCCTCTACAG TTTCCAGTTC GACGACTAGT ACCAGTGCGA ATCCCGCGGC TGTCAAACCG 
ATGTTCAGCT GGTCCGGAGC TGGCAGTGCC GTGAGCAGCA CCAGTAGCAG CCAGCAGCCA 
GTGGCCAAGG CGCCAACCCT AGGTTTTGGT GTTTCCTCCA GCACCGTAAC AACGACAACA 
ACCTCTACGA AAGTTTTTGC TTTCACACCG GCATCTGGTC TAGATCCGGC AGCAGCCACC 
TCGGCACCAG CTGCAGGTGC CGGCTTCAGT TTTGGAAGTC AGTCAAAGCC AGCAACCACA 
CAGAACACTG GAACCTTCTT CTTTGGCCAG CCAACAGCGG TTGCGCCAGC AACGCCAACC 
AACCCTAGCG TGAGCTCCAT CTTCGGAGCA CCGGCAACAA GTACCACTGC AAGCACCAGC 
GTGAGTGCCA CCACCAGCAC CAGCACCGCC AACGCCATTG CTTCCAGCTT TGCGCCCACC 
TCGACCCCTC AGCTCTTTGG CAATTGGGGT GAAAAGAAAA CGGACTTGAC CACCTTTGGT 
GCTTCTTCCG GTTCGGGAAC GACTACAACG CCTAGCTTCG GCTGGTCGAG CAATGGAGAT 
GCGGCAAAGA GCAACAGCGC TGCGGTCGGC AGTGCGGCGG TGCCTTCGTC TTCGGCCTCC 
ACGATGGCGA CTCCGATATT CGGCAGCAGT TCCATGTTTG GACCATCCAG TAGCAGTAAC 
AACACCACCA GCACCAGCAC CACTAGTCTA CCATTCGGAT CAGCGGCGAC CACTGCCGCA 
ACTACGCCGG CGGGCGGCAA TGCAGCCCTT ACTGGGTTAT TCGGCAATGT GGGCAACTCT 
CTAGCGGGAG TAGGAGCTCC TGTAGCCACA ACACCAGCGG CAACAGCTGC TGCTCCATTG 
ACCAACATCT TTGGCAATCC CACGCCAGTT GCTGCTGCCG CGCCTGTCTT CGGCAGCGGA 
AGCACCATCC CAAGCGCAGG ATTTGGAGCG CCTGCTGCAG CTGCTCCGTT GGCGGCCCCA 
GCGTTACCAG GAGCATTTAA CTTTGGCGGA GCAACTGCAG CCACGCCAGC TGCCTCAAGT 
GCTCCATTTG TATTCGGAAG CAGCACCAAT GAGCCGCTGG CCAAACCGTC GTTCAACTTC 
ACCGGCTCGG CGGCCAGCTC GACTGCTCCT GCGCCAGCCT TCAATTTCAC GGCCAATACG 
GCGGCCACTA ATAATCCCTC CGGTGGCGAT TCGCATCCGC GCATCTTCCA ATTCGGTAGT 
TCGCAACCGG CGGCCAATAA CCAGTTTGGT GGTGCGGGCG GTGCCACCAA CAACTCCGCC 
GGCGGTATGC CCATGTTCAA CTTTTCGCCC GGGGCGCCGC AGATGCAGTC CACACCGAAT 
GCCAATGCAC TGTTCCAATT TAGCGCGACC TCCACAGCGC CAGCCAATAT ATTTGCCTTC 
AATCCGCCAG CTGCGGGCAA CTCGGCGCAG AGTTCCCAGA CGGCTCGCCG CAAAATCCGC 
GCCCCCGTAC GCCGTTTGCC GCCCCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Nup153 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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