Pp2B-14D cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pp2B-14D
Entrez Gene ID	32624
Full Name	Protein phosphatase 2B at 14D
Synonyms	CG9842,CaNA,CanA,CanA14D,CnA,CnnA14D,D33,DmPp2B-14D,Dmel\CG9842,PP2B,PP2B 14D,PP2B-14D,Pp12-14D,Pp2B-14D/CaN,Pp2B-14DF,Pp2b-14D,PpD33,canA
General protein information	Preferred Names Protein phosphatase 2B at 14D Names CG9842 gene product from transcript CG9842-RC CG9842-PA CG9842-PB CG9842-PC Pp2B-14D-PA Pp2B-14D-PB Pp2B-14D-PC calcineurin protein phosphatase 2B at 14D protein phosphatase D33 protein phosphatase from PCR fragment D33
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 14E1-14E3

mRNA and Protein(s)

mRNA	Protein	Name
NM_078649.4	NP_523373.2	protein phosphatase 2B at 14D, isoform A
NM_001258787.2	NP_001245716.1	protein phosphatase 2B at 14D, isoform B
NM_001258786.3	NP_001245715.1	protein phosphatase 2B at 14D, isoform C

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PPP3CA	NP_001184025.1
Rattus norvegicus (Norway rat)	Ppp3ca	NP_058737.1
Danio rerio (zebrafish)	ppp3cb	NP_001017856.1
Saccharomyces cerevisiae (baker's yeast)	CNA1	NP_013537.1
Neurospora crassa	NCU03804	XP_961193.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001279	XP_321872.5
Kluyveromyces lactis	KLLA0B05489g	XP_451780.1
Eremothecium gossypii	AGOS_AER118C	NP_984978.1
Magnaporthe oryzae (rice blast fungus)	MGG_07456	XP_003711354.1
Bos taurus (cattle)	PPP3CA	NP_777212.1
Danio rerio (zebrafish)	ppp3ca	NP_001185479.1
Schizosaccharomyces pombe (fission yeast)	ppb1	NP_596178.1
Xenopus tropicalis (tropical clawed frog)	ppp3ca	NP_001119980.1
Pan troglodytes (chimpanzee)	PPP3CA	XP_001168163.1
Mus musculus (house mouse)	Ppp3ca	NP_032939.1
Drosophila melanogaster (fruit fly)	CanA-14F	NP_001259622.1
Homo sapiens (human)	PPP3CA	NP_000935.1
	PPP3CA	XP_001108659.1
Gallus gallus (chicken)	PPP3CA	XP_420664.4
Drosophila melanogaster (fruit fly)	Pp2B-14D	NP_523373.2
Caenorhabditis elegans (roundworm)	tax-6	NP_001076658.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pp2B-14D gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pp2B-14D cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01829	NM_078649.4 Latest version!	Drosophila melanogaster protein phosphatase 2B at 14D, transcript variant A (Pp2B-14D), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa01829	NM_001258786.3 Latest version!	Drosophila melanogaster protein phosphatase 2B at 14D, transcript variant C (Pp2B-14D), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa01829	NM_001258787.2 Latest version!	Drosophila melanogaster protein phosphatase 2B at 14D, transcript variant B (Pp2B-14D), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pp2B-14D ( NM_078649.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523373.2 Drosophila melanogaster protein phosphatase 2B at 14D, transcript variant A (Pp2B-14D), mRNA.

Price & Availability↑

CloneID	OFa01829
Clone ID Related Accession (Same CDS sequence)	NM_078649.4 , NM_001258787.2 , NM_001258786.3
Accession Version	NM_078649.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	protein phosphatase 2B at 14D, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_078649.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTTCGA ATAACCAGAG CAGCAGCGTT GCCCAGGCGG CAACAAGTGC CCGCACCGTC 
AGTGCCGGAT CGGCGGAAGC CACAGATGCC AATAGTACCG CCTCGAACAA CAACAATAAT 
AGCAGCAGTA CCGCTGCAGC GGGCAACAAC AGCGACAACA GCAGTCCCAC CACTGGAACG 
GGCACAGGAG CGAGCACTGG AAAGCTGCAC GGCGGCCACA CGGCAGTAAA CACCAAGGAA 
CGGGTGGTGG ACAGCGTTCC CTTCCCGCCC AGCCACAAGC TGACCTTGGC GGAGGTGTTC 
GACCAACGCA CCGGCAAGCC CAACCATGAG CTGCTCAAGC AGCACTTCAT TCTTGAGGGC 
AGAATCGAGG AGGCGCCCGC CCTGAAAATC ATCCAGGACG GAGCCGCCCT GCTGCGTCAG 
GAGAAGACGA TGATCGATAT TGAGGCGCCG GTGACGGTGT GCGGTGATAT CCACGGCCAG 
TTCTACGACC TGATGAAGCT GTTCGAAGTG GGCGGCTCGC CCGCGAGCAC CAAGTATCTG 
TTCCTGGGCG ACTACGTCGA TCGGGGCTAC TTCAGCATCG AGTGCGTCCT GTATTTGTGG 
TCGCTAAAGA TCACCTATCC GCAGACGCTG TTCCTGCTGC GCGGCAACCA CGAGTGCCGG 
CACTTAACCG AGTACTTCAC CTTCAAGCAG GAGTGCAAGA TCAAGTACTC GGAGCGCGTG 
TACGACGCCT GCATGGACGC ATTCGACTGC CTGCCGCTGG CGGCGCTAAT GAACCAGCAG 
TTCCTCTGCG TGCACGGCGG TCTGTCGCCG GAGATACACG AGCTGGAGGA CATCCGGCGG 
CTCGACCGCT TCAAGGAGCC GCCCGCCTTT GGCCCCATGT GCGACCTGCT GTGGTCCGAT 
CCCCTGGAGG ACTTTGGCAA CGAGAAGAAC TCGGACTTCT ACACGCACAA CTCCGTGCGC 
GGATGCTCGT ACTTCTACAG CTACGCCGCC TGCTGCGACT TCCTGCAGAA CAACAACCTG 
CTGTCGATCA TCCGGGCGCA CGAGGCGCAG GACGCCGGCT ACCGCATGTA CCGCAAAAGC 
CAGACCACCG GCTTCCCCTC GCTGATCACC ATCTTCTCGG CGCCCAACTA TCTCGACGTG 
TACAACAACA AGGCGGCGGT GCTGAAGTAC GAGAACAACG TGATGAACAT CCGGCAATTC 
AACTGCTCGC CGCACCCGTA CTGGCTGCCC AACTTCATGG ACGTGTTCAC CTGGTCGCTA 
CCCTTCGTGG GCGAGAAGGT CACCGAGATG TTGGTGAACG TGCTGAACAT CTGCTCCGAC 
GACGAGCTCA TGACCGAGGA GAGCGAGGAG CCGCTCTCCG ACGACGAGGC GGCGCTGCGC 
AAGGAGGTGA TACGCAACAA GATCCGTGCC ATTGGCAAGA TGGCGCGCGT CTTCTCCGTG 
CTGCGCGAGG AGTCCGAGTC GGTGCTGCAG CTGAAGGGCC TGACGCCCAC GGGCGCCCTG 
CCCCTCGGCG CCCTCTCCGG CGGCAAGCAG TCGCTCAAGA ACGCCATGCA GGGCTTCTCG 
CCCAACCACA AGATTACCTC GTTCGCGGAG GCCAAGGGCC TGGATGCCGT CAACGAACGG 
ATGCCGCCGC GGCGGGATCA GCCGCCCACG CCCAGCGAGG ATCCGAATCA GCACAGTCAG 
CAGGGCGGCA AAAATGGGGC TGGACATGGG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523373.2
CDS	610..2322
Translation	MSSNNQSSSVAQAATSARTVSAGSAEATDANSTASNNNNNSSSTAAAGNNSDNSSPTTGTGTGASTGKLHGGHTAVNTKERVVDSVPFPPSHKLTLAEVFDQRTGKPNHELLKQHFILEGRIEEAPALKIIQDGAALLRQEKTMIDIEAPVTVCGDIHGQFYDLMKLFEVGGSPASTKYLFLGDYVDRGYFSIECVLYLWSLKITYPQTLFLLRGNHECRHLTEYFTFKQECKIKYSERVYDACMDAFDCLPLAALMNQQFLCVHGGLSPEIHELEDIRRLDRFKEPPAFGPMCDLLWSDPLEDFGNEKNSDFYTHNSVRGCSYFYSYAACCDFLQNNNLLSIIRAHEAQDAGYRMYRKSQTTGFPSLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVGEKVTEMLVNVLNICSDDELMTEESEEPLSDDEAALRKEVIRNKIRAIGKMARVFSVLREESESVLQLKGLTPTGALPLGALSGGKQSLKNAMQGFSPNHKITSFAEAKGLDAVNERMPPRRDQPPTPSEDPNQHSQQGGKNGAGHG

Target ORF information:

RefSeq Version	NM_078649.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster protein phosphatase 2B at 14D, transcript variant A (Pp2B-14D), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078649.4

ORF Insert Sequence:

ATGTCTTCGA ATAACCAGAG CAGCAGCGTT GCCCAGGCGG CAACAAGTGC CCGCACCGTC 
AGTGCCGGAT CGGCGGAAGC CACAGATGCC AATAGTACCG CCTCGAACAA CAACAATAAT 
AGCAGCAGTA CCGCTGCAGC GGGCAACAAC AGCGACAACA GCAGTCCCAC CACTGGAACG 
GGCACAGGAG CGAGCACTGG AAAGCTGCAC GGCGGCCACA CGGCAGTAAA CACCAAGGAA 
CGGGTGGTGG ACAGCGTTCC CTTCCCGCCC AGCCACAAGC TGACCTTGGC GGAGGTGTTC 
GACCAACGCA CCGGCAAGCC CAACCATGAG CTGCTCAAGC AGCACTTCAT TCTTGAGGGC 
AGAATCGAGG AGGCGCCCGC CCTGAAAATC ATCCAGGACG GAGCCGCCCT GCTGCGTCAG 
GAGAAGACGA TGATCGATAT TGAGGCGCCG GTGACGGTGT GCGGTGATAT CCACGGCCAG 
TTCTACGACC TGATGAAGCT GTTCGAAGTG GGCGGCTCGC CCGCGAGCAC CAAGTATCTG 
TTCCTGGGCG ACTACGTCGA TCGGGGCTAC TTCAGCATCG AGTGCGTCCT GTATTTGTGG 
TCGCTAAAGA TCACCTATCC GCAGACGCTG TTCCTGCTGC GCGGCAACCA CGAGTGCCGG 
CACTTAACCG AGTACTTCAC CTTCAAGCAG GAGTGCAAGA TCAAGTACTC GGAGCGCGTG 
TACGACGCCT GCATGGACGC ATTCGACTGC CTGCCGCTGG CGGCGCTAAT GAACCAGCAG 
TTCCTCTGCG TGCACGGCGG TCTGTCGCCG GAGATACACG AGCTGGAGGA CATCCGGCGG 
CTCGACCGCT TCAAGGAGCC GCCCGCCTTT GGCCCCATGT GCGACCTGCT GTGGTCCGAT 
CCCCTGGAGG ACTTTGGCAA CGAGAAGAAC TCGGACTTCT ACACGCACAA CTCCGTGCGC 
GGATGCTCGT ACTTCTACAG CTACGCCGCC TGCTGCGACT TCCTGCAGAA CAACAACCTG 
CTGTCGATCA TCCGGGCGCA CGAGGCGCAG GACGCCGGCT ACCGCATGTA CCGCAAAAGC 
CAGACCACCG GCTTCCCCTC GCTGATCACC ATCTTCTCGG CGCCCAACTA TCTCGACGTG 
TACAACAACA AGGCGGCGGT GCTGAAGTAC GAGAACAACG TGATGAACAT CCGGCAATTC 
AACTGCTCGC CGCACCCGTA CTGGCTGCCC AACTTCATGG ACGTGTTCAC CTGGTCGCTA 
CCCTTCGTGG GCGAGAAGGT CACCGAGATG TTGGTGAACG TGCTGAACAT CTGCTCCGAC 
GACGAGCTCA TGACCGAGGA GAGCGAGGAG CCGCTCTCCG ACGACGAGGC GGCGCTGCGC 
AAGGAGGTGA TACGCAACAA GATCCGTGCC ATTGGCAAGA TGGCGCGCGT CTTCTCCGTG 
CTGCGCGAGG AGTCCGAGTC GGTGCTGCAG CTGAAGGGCC TGACGCCCAC GGGCGCCCTG 
CCCCTCGGCG CCCTCTCCGG CGGCAAGCAG TCGCTCAAGA ACGCCATGCA GGGCTTCTCG 
CCCAACCACA AGATTACCTC GTTCGCGGAG GCCAAGGGCC TGGATGCCGT CAACGAACGG 
ATGCCGCCGC GGCGGGATCA GCCGCCCACG CCCAGCGAGG ATCCGAATCA GCACAGTCAG 
CAGGGCGGCA AAAATGGGGC TGGACATGGG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pp2B-14D ( NM_001258787.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245716.1 Drosophila melanogaster protein phosphatase 2B at 14D, transcript variant B (Pp2B-14D), mRNA.

Price & Availability↑

CloneID	OFa01829
Clone ID Related Accession (Same CDS sequence)	NM_078649.4 , NM_001258787.2 , NM_001258786.3
Accession Version	NM_001258787.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	protein phosphatase 2B at 14D, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001258787.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTTCGA ATAACCAGAG CAGCAGCGTT GCCCAGGCGG CAACAAGTGC CCGCACCGTC 
AGTGCCGGAT CGGCGGAAGC CACAGATGCC AATAGTACCG CCTCGAACAA CAACAATAAT 
AGCAGCAGTA CCGCTGCAGC GGGCAACAAC AGCGACAACA GCAGTCCCAC CACTGGAACG 
GGCACAGGAG CGAGCACTGG AAAGCTGCAC GGCGGCCACA CGGCAGTAAA CACCAAGGAA 
CGGGTGGTGG ACAGCGTTCC CTTCCCGCCC AGCCACAAGC TGACCTTGGC GGAGGTGTTC 
GACCAACGCA CCGGCAAGCC CAACCATGAG CTGCTCAAGC AGCACTTCAT TCTTGAGGGC 
AGAATCGAGG AGGCGCCCGC CCTGAAAATC ATCCAGGACG GAGCCGCCCT GCTGCGTCAG 
GAGAAGACGA TGATCGATAT TGAGGCGCCG GTGACGGTGT GCGGTGATAT CCACGGCCAG 
TTCTACGACC TGATGAAGCT GTTCGAAGTG GGCGGCTCGC CCGCGAGCAC CAAGTATCTG 
TTCCTGGGCG ACTACGTCGA TCGGGGCTAC TTCAGCATCG AGTGCGTCCT GTATTTGTGG 
TCGCTAAAGA TCACCTATCC GCAGACGCTG TTCCTGCTGC GCGGCAACCA CGAGTGCCGG 
CACTTAACCG AGTACTTCAC CTTCAAGCAG GAGTGCAAGA TCAAGTACTC GGAGCGCGTG 
TACGACGCCT GCATGGACGC ATTCGACTGC CTGCCGCTGG CGGCGCTAAT GAACCAGCAG 
TTCCTCTGCG TGCACGGCGG TCTGTCGCCG GAGATACACG AGCTGGAGGA CATCCGGCGG 
CTCGACCGCT TCAAGGAGCC GCCCGCCTTT GGCCCCATGT GCGACCTGCT GTGGTCCGAT 
CCCCTGGAGG ACTTTGGCAA CGAGAAGAAC TCGGACTTCT ACACGCACAA CTCCGTGCGC 
GGATGCTCGT ACTTCTACAG CTACGCCGCC TGCTGCGACT TCCTGCAGAA CAACAACCTG 
CTGTCGATCA TCCGGGCGCA CGAGGCGCAG GACGCCGGCT ACCGCATGTA CCGCAAAAGC 
CAGACCACCG GCTTCCCCTC GCTGATCACC ATCTTCTCGG CGCCCAACTA TCTCGACGTG 
TACAACAACA AGGCGGCGGT GCTGAAGTAC GAGAACAACG TGATGAACAT CCGGCAATTC 
AACTGCTCGC CGCACCCGTA CTGGCTGCCC AACTTCATGG ACGTGTTCAC CTGGTCGCTA 
CCCTTCGTGG GCGAGAAGGT CACCGAGATG TTGGTGAACG TGCTGAACAT CTGCTCCGAC 
GACGAGCTCA TGACCGAGGA GAGCGAGGAG CCGCTCTCCG ACGACGAGGC GGCGCTGCGC 
AAGGAGGTGA TACGCAACAA GATCCGTGCC ATTGGCAAGA TGGCGCGCGT CTTCTCCGTG 
CTGCGCGAGG AGTCCGAGTC GGTGCTGCAG CTGAAGGGCC TGACGCCCAC GGGCGCCCTG 
CCCCTCGGCG CCCTCTCCGG CGGCAAGCAG TCGCTCAAGA ACGCCATGCA GGGCTTCTCG 
CCCAACCACA AGATTACCTC GTTCGCGGAG GCCAAGGGCC TGGATGCCGT CAACGAACGG 
ATGCCGCCGC GGCGGGATCA GCCGCCCACG CCCAGCGAGG ATCCGAATCA GCACAGTCAG 
CAGGGCGGCA AAAATGGGGC TGGACATGGG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245716.1
CDS	610..2322
Translation	MSSNNQSSSVAQAATSARTVSAGSAEATDANSTASNNNNNSSSTAAAGNNSDNSSPTTGTGTGASTGKLHGGHTAVNTKERVVDSVPFPPSHKLTLAEVFDQRTGKPNHELLKQHFILEGRIEEAPALKIIQDGAALLRQEKTMIDIEAPVTVCGDIHGQFYDLMKLFEVGGSPASTKYLFLGDYVDRGYFSIECVLYLWSLKITYPQTLFLLRGNHECRHLTEYFTFKQECKIKYSERVYDACMDAFDCLPLAALMNQQFLCVHGGLSPEIHELEDIRRLDRFKEPPAFGPMCDLLWSDPLEDFGNEKNSDFYTHNSVRGCSYFYSYAACCDFLQNNNLLSIIRAHEAQDAGYRMYRKSQTTGFPSLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVGEKVTEMLVNVLNICSDDELMTEESEEPLSDDEAALRKEVIRNKIRAIGKMARVFSVLREESESVLQLKGLTPTGALPLGALSGGKQSLKNAMQGFSPNHKITSFAEAKGLDAVNERMPPRRDQPPTPSEDPNQHSQQGGKNGAGHG

Target ORF information:

RefSeq Version	NM_001258787.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster protein phosphatase 2B at 14D, transcript variant B (Pp2B-14D), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258787.2

ORF Insert Sequence:

ATGTCTTCGA ATAACCAGAG CAGCAGCGTT GCCCAGGCGG CAACAAGTGC CCGCACCGTC 
AGTGCCGGAT CGGCGGAAGC CACAGATGCC AATAGTACCG CCTCGAACAA CAACAATAAT 
AGCAGCAGTA CCGCTGCAGC GGGCAACAAC AGCGACAACA GCAGTCCCAC CACTGGAACG 
GGCACAGGAG CGAGCACTGG AAAGCTGCAC GGCGGCCACA CGGCAGTAAA CACCAAGGAA 
CGGGTGGTGG ACAGCGTTCC CTTCCCGCCC AGCCACAAGC TGACCTTGGC GGAGGTGTTC 
GACCAACGCA CCGGCAAGCC CAACCATGAG CTGCTCAAGC AGCACTTCAT TCTTGAGGGC 
AGAATCGAGG AGGCGCCCGC CCTGAAAATC ATCCAGGACG GAGCCGCCCT GCTGCGTCAG 
GAGAAGACGA TGATCGATAT TGAGGCGCCG GTGACGGTGT GCGGTGATAT CCACGGCCAG 
TTCTACGACC TGATGAAGCT GTTCGAAGTG GGCGGCTCGC CCGCGAGCAC CAAGTATCTG 
TTCCTGGGCG ACTACGTCGA TCGGGGCTAC TTCAGCATCG AGTGCGTCCT GTATTTGTGG 
TCGCTAAAGA TCACCTATCC GCAGACGCTG TTCCTGCTGC GCGGCAACCA CGAGTGCCGG 
CACTTAACCG AGTACTTCAC CTTCAAGCAG GAGTGCAAGA TCAAGTACTC GGAGCGCGTG 
TACGACGCCT GCATGGACGC ATTCGACTGC CTGCCGCTGG CGGCGCTAAT GAACCAGCAG 
TTCCTCTGCG TGCACGGCGG TCTGTCGCCG GAGATACACG AGCTGGAGGA CATCCGGCGG 
CTCGACCGCT TCAAGGAGCC GCCCGCCTTT GGCCCCATGT GCGACCTGCT GTGGTCCGAT 
CCCCTGGAGG ACTTTGGCAA CGAGAAGAAC TCGGACTTCT ACACGCACAA CTCCGTGCGC 
GGATGCTCGT ACTTCTACAG CTACGCCGCC TGCTGCGACT TCCTGCAGAA CAACAACCTG 
CTGTCGATCA TCCGGGCGCA CGAGGCGCAG GACGCCGGCT ACCGCATGTA CCGCAAAAGC 
CAGACCACCG GCTTCCCCTC GCTGATCACC ATCTTCTCGG CGCCCAACTA TCTCGACGTG 
TACAACAACA AGGCGGCGGT GCTGAAGTAC GAGAACAACG TGATGAACAT CCGGCAATTC 
AACTGCTCGC CGCACCCGTA CTGGCTGCCC AACTTCATGG ACGTGTTCAC CTGGTCGCTA 
CCCTTCGTGG GCGAGAAGGT CACCGAGATG TTGGTGAACG TGCTGAACAT CTGCTCCGAC 
GACGAGCTCA TGACCGAGGA GAGCGAGGAG CCGCTCTCCG ACGACGAGGC GGCGCTGCGC 
AAGGAGGTGA TACGCAACAA GATCCGTGCC ATTGGCAAGA TGGCGCGCGT CTTCTCCGTG 
CTGCGCGAGG AGTCCGAGTC GGTGCTGCAG CTGAAGGGCC TGACGCCCAC GGGCGCCCTG 
CCCCTCGGCG CCCTCTCCGG CGGCAAGCAG TCGCTCAAGA ACGCCATGCA GGGCTTCTCG 
CCCAACCACA AGATTACCTC GTTCGCGGAG GCCAAGGGCC TGGATGCCGT CAACGAACGG 
ATGCCGCCGC GGCGGGATCA GCCGCCCACG CCCAGCGAGG ATCCGAATCA GCACAGTCAG 
CAGGGCGGCA AAAATGGGGC TGGACATGGG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pp2B-14D ( NM_001258786.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245715.1 Drosophila melanogaster protein phosphatase 2B at 14D, transcript variant C (Pp2B-14D), mRNA.

Price & Availability↑

CloneID	OFa01829
Clone ID Related Accession (Same CDS sequence)	NM_078649.4 , NM_001258787.2 , NM_001258786.3
Accession Version	NM_001258786.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	protein phosphatase 2B at 14D, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001258786.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTTCGA ATAACCAGAG CAGCAGCGTT GCCCAGGCGG CAACAAGTGC CCGCACCGTC 
AGTGCCGGAT CGGCGGAAGC CACAGATGCC AATAGTACCG CCTCGAACAA CAACAATAAT 
AGCAGCAGTA CCGCTGCAGC GGGCAACAAC AGCGACAACA GCAGTCCCAC CACTGGAACG 
GGCACAGGAG CGAGCACTGG AAAGCTGCAC GGCGGCCACA CGGCAGTAAA CACCAAGGAA 
CGGGTGGTGG ACAGCGTTCC CTTCCCGCCC AGCCACAAGC TGACCTTGGC GGAGGTGTTC 
GACCAACGCA CCGGCAAGCC CAACCATGAG CTGCTCAAGC AGCACTTCAT TCTTGAGGGC 
AGAATCGAGG AGGCGCCCGC CCTGAAAATC ATCCAGGACG GAGCCGCCCT GCTGCGTCAG 
GAGAAGACGA TGATCGATAT TGAGGCGCCG GTGACGGTGT GCGGTGATAT CCACGGCCAG 
TTCTACGACC TGATGAAGCT GTTCGAAGTG GGCGGCTCGC CCGCGAGCAC CAAGTATCTG 
TTCCTGGGCG ACTACGTCGA TCGGGGCTAC TTCAGCATCG AGTGCGTCCT GTATTTGTGG 
TCGCTAAAGA TCACCTATCC GCAGACGCTG TTCCTGCTGC GCGGCAACCA CGAGTGCCGG 
CACTTAACCG AGTACTTCAC CTTCAAGCAG GAGTGCAAGA TCAAGTACTC GGAGCGCGTG 
TACGACGCCT GCATGGACGC ATTCGACTGC CTGCCGCTGG CGGCGCTAAT GAACCAGCAG 
TTCCTCTGCG TGCACGGCGG TCTGTCGCCG GAGATACACG AGCTGGAGGA CATCCGGCGG 
CTCGACCGCT TCAAGGAGCC GCCCGCCTTT GGCCCCATGT GCGACCTGCT GTGGTCCGAT 
CCCCTGGAGG ACTTTGGCAA CGAGAAGAAC TCGGACTTCT ACACGCACAA CTCCGTGCGC 
GGATGCTCGT ACTTCTACAG CTACGCCGCC TGCTGCGACT TCCTGCAGAA CAACAACCTG 
CTGTCGATCA TCCGGGCGCA CGAGGCGCAG GACGCCGGCT ACCGCATGTA CCGCAAAAGC 
CAGACCACCG GCTTCCCCTC GCTGATCACC ATCTTCTCGG CGCCCAACTA TCTCGACGTG 
TACAACAACA AGGCGGCGGT GCTGAAGTAC GAGAACAACG TGATGAACAT CCGGCAATTC 
AACTGCTCGC CGCACCCGTA CTGGCTGCCC AACTTCATGG ACGTGTTCAC CTGGTCGCTA 
CCCTTCGTGG GCGAGAAGGT CACCGAGATG TTGGTGAACG TGCTGAACAT CTGCTCCGAC 
GACGAGCTCA TGACCGAGGA GAGCGAGGAG CCGCTCTCCG ACGACGAGGC GGCGCTGCGC 
AAGGAGGTGA TACGCAACAA GATCCGTGCC ATTGGCAAGA TGGCGCGCGT CTTCTCCGTG 
CTGCGCGAGG AGTCCGAGTC GGTGCTGCAG CTGAAGGGCC TGACGCCCAC GGGCGCCCTG 
CCCCTCGGCG CCCTCTCCGG CGGCAAGCAG TCGCTCAAGA ACGCCATGCA GGGCTTCTCG 
CCCAACCACA AGATTACCTC GTTCGCGGAG GCCAAGGGCC TGGATGCCGT CAACGAACGG 
ATGCCGCCGC GGCGGGATCA GCCGCCCACG CCCAGCGAGG ATCCGAATCA GCACAGTCAG 
CAGGGCGGCA AAAATGGGGC TGGACATGGG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245715.1
CDS	610..2322
Translation	MSSNNQSSSVAQAATSARTVSAGSAEATDANSTASNNNNNSSSTAAAGNNSDNSSPTTGTGTGASTGKLHGGHTAVNTKERVVDSVPFPPSHKLTLAEVFDQRTGKPNHELLKQHFILEGRIEEAPALKIIQDGAALLRQEKTMIDIEAPVTVCGDIHGQFYDLMKLFEVGGSPASTKYLFLGDYVDRGYFSIECVLYLWSLKITYPQTLFLLRGNHECRHLTEYFTFKQECKIKYSERVYDACMDAFDCLPLAALMNQQFLCVHGGLSPEIHELEDIRRLDRFKEPPAFGPMCDLLWSDPLEDFGNEKNSDFYTHNSVRGCSYFYSYAACCDFLQNNNLLSIIRAHEAQDAGYRMYRKSQTTGFPSLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVGEKVTEMLVNVLNICSDDELMTEESEEPLSDDEAALRKEVIRNKIRAIGKMARVFSVLREESESVLQLKGLTPTGALPLGALSGGKQSLKNAMQGFSPNHKITSFAEAKGLDAVNERMPPRRDQPPTPSEDPNQHSQQGGKNGAGHG

Target ORF information:

RefSeq Version	NM_001258786.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster protein phosphatase 2B at 14D, transcript variant C (Pp2B-14D), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258786.3

ORF Insert Sequence:

ATGTCTTCGA ATAACCAGAG CAGCAGCGTT GCCCAGGCGG CAACAAGTGC CCGCACCGTC 
AGTGCCGGAT CGGCGGAAGC CACAGATGCC AATAGTACCG CCTCGAACAA CAACAATAAT 
AGCAGCAGTA CCGCTGCAGC GGGCAACAAC AGCGACAACA GCAGTCCCAC CACTGGAACG 
GGCACAGGAG CGAGCACTGG AAAGCTGCAC GGCGGCCACA CGGCAGTAAA CACCAAGGAA 
CGGGTGGTGG ACAGCGTTCC CTTCCCGCCC AGCCACAAGC TGACCTTGGC GGAGGTGTTC 
GACCAACGCA CCGGCAAGCC CAACCATGAG CTGCTCAAGC AGCACTTCAT TCTTGAGGGC 
AGAATCGAGG AGGCGCCCGC CCTGAAAATC ATCCAGGACG GAGCCGCCCT GCTGCGTCAG 
GAGAAGACGA TGATCGATAT TGAGGCGCCG GTGACGGTGT GCGGTGATAT CCACGGCCAG 
TTCTACGACC TGATGAAGCT GTTCGAAGTG GGCGGCTCGC CCGCGAGCAC CAAGTATCTG 
TTCCTGGGCG ACTACGTCGA TCGGGGCTAC TTCAGCATCG AGTGCGTCCT GTATTTGTGG 
TCGCTAAAGA TCACCTATCC GCAGACGCTG TTCCTGCTGC GCGGCAACCA CGAGTGCCGG 
CACTTAACCG AGTACTTCAC CTTCAAGCAG GAGTGCAAGA TCAAGTACTC GGAGCGCGTG 
TACGACGCCT GCATGGACGC ATTCGACTGC CTGCCGCTGG CGGCGCTAAT GAACCAGCAG 
TTCCTCTGCG TGCACGGCGG TCTGTCGCCG GAGATACACG AGCTGGAGGA CATCCGGCGG 
CTCGACCGCT TCAAGGAGCC GCCCGCCTTT GGCCCCATGT GCGACCTGCT GTGGTCCGAT 
CCCCTGGAGG ACTTTGGCAA CGAGAAGAAC TCGGACTTCT ACACGCACAA CTCCGTGCGC 
GGATGCTCGT ACTTCTACAG CTACGCCGCC TGCTGCGACT TCCTGCAGAA CAACAACCTG 
CTGTCGATCA TCCGGGCGCA CGAGGCGCAG GACGCCGGCT ACCGCATGTA CCGCAAAAGC 
CAGACCACCG GCTTCCCCTC GCTGATCACC ATCTTCTCGG CGCCCAACTA TCTCGACGTG 
TACAACAACA AGGCGGCGGT GCTGAAGTAC GAGAACAACG TGATGAACAT CCGGCAATTC 
AACTGCTCGC CGCACCCGTA CTGGCTGCCC AACTTCATGG ACGTGTTCAC CTGGTCGCTA 
CCCTTCGTGG GCGAGAAGGT CACCGAGATG TTGGTGAACG TGCTGAACAT CTGCTCCGAC 
GACGAGCTCA TGACCGAGGA GAGCGAGGAG CCGCTCTCCG ACGACGAGGC GGCGCTGCGC 
AAGGAGGTGA TACGCAACAA GATCCGTGCC ATTGGCAAGA TGGCGCGCGT CTTCTCCGTG 
CTGCGCGAGG AGTCCGAGTC GGTGCTGCAG CTGAAGGGCC TGACGCCCAC GGGCGCCCTG 
CCCCTCGGCG CCCTCTCCGG CGGCAAGCAG TCGCTCAAGA ACGCCATGCA GGGCTTCTCG 
CCCAACCACA AGATTACCTC GTTCGCGGAG GCCAAGGGCC TGGATGCCGT CAACGAACGG 
ATGCCGCCGC GGCGGGATCA GCCGCCCACG CCCAGCGAGG ATCCGAATCA GCACAGTCAG 
CAGGGCGGCA AAAATGGGGC TGGACATGGG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Pp2B-14D cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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