CG32344 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG32344
Entrez Gene ID	326208
Full Name	CG32344 gene product from transcript CG32344-RA
Synonyms	CG6994,DmRH20,Dmel\CG32344,cg6994
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 61C1-61C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_138184.5	NP_612028.4	uncharacterized protein

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ddx54	NP_082317.1
Danio rerio (zebrafish)	ddx54	NP_775375.1
	DDX54	XP_001111246.1
Gallus gallus (chicken)	DDX54	XP_003642273.2
Caenorhabditis elegans (roundworm)	Y94H6A.5	NP_741347.2
Saccharomyces cerevisiae (baker's yeast)	DBP10	NP_010253.2
Homo sapiens (human)	DDX54	NP_001104792.1
Pan troglodytes (chimpanzee)	DDX54	XP_001152281.1
Bos taurus (cattle)	DDX54	XP_005218062.1
Neurospora crassa	NCU07712	XP_962298.1
Arabidopsis thaliana (thale cress)	AT1G77030	NP_177829.5
Xenopus tropicalis (tropical clawed frog)	ddx54	XP_002934380.1
Canis lupus familiaris (dog)	DDX54	XP_005636568.1
Rattus norvegicus (Norway rat)	Ddx54	NP_001178477.1
Drosophila melanogaster (fruit fly)	CG32344	NP_612028.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007511	XP_308366.3
Kluyveromyces lactis	KLLA0F24684g	XP_456179.1
Eremothecium gossypii	AGOS_AEL086W	NP_984775.1
Schizosaccharomyces pombe (fission yeast)	dbp10	NP_592919.1
Magnaporthe oryzae (rice blast fungus)	MGG_04179	XP_003719645.1
Oryza sativa (rice)	Os08g0416100	NP_001061808.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG32344 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG32344 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa19594	NM_138184.5 Latest version!	Drosophila melanogaster uncharacterized protein (CG32344), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG32344 ( NM_138184.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_612028.4 Drosophila melanogaster uncharacterized protein (CG32344), mRNA.

Price & Availability↑

CloneID	OFa19594
Clone ID Related Accession (Same CDS sequence)	NM_138184.5
Accession Version	NM_138184.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_138184.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGTAAGA AGCAGGCGGA TGAAATACCA GGCTTTCCAT CACTGGACAA CGATGCCGGC 
ACCAGCGACC GGGGCGCCGA CATTCTCAAG TCCAAGTCCA AGAAGAACAA GAGTGGTGGA 
TTCCAGTCGA TGGGCCTGGG CTTCGAGCTG ATAAAGGGCA TCACCAAGCG GGGTTACAAG 
GTTCCCACCC CCATACAGCG GAAAACGATT CCGCTGATCC TGGAGGGACG CGATGTGGTG 
GCAATGGCCA AGACGGGCTC TGGCAAGACG GCCTGCTTTC TGATTCCGCT CTTTGAAAAG 
CTACAGAGGC GCGAGCCCAC AAAGGGGGCC CGTGCCCTGA TCCTGTCTCC GACGCGCGAA 
CTGGCTGTGC AGACGTATAA GTTCATAAAG GAGCTGGGCC GCTTTATGGA ACTGAAGAGC 
ATTCTGGTGC TAGGTGGCGA CTCCATGGAC TCACAGTTCT CGGCCATACA TACCTGTCCG 
GACGTAATTG TTGCAACGCC AGGTCGTTTT CTTCATTTGT GCGTAGAAAT GGACTTGAAG 
CTAAATTCCA TAGAGTACGT GGTGTTTGAT GAAGCCGATC GACTCTTCGA GATGGGCTTC 
GGCGAGCAAC TGAACGAAAC GTTGCACAGA TTGCCATCGT CCCGGCAGAC GGTCATGTTT 
TCAGCCACGC TGCCAAAACT GCTAGTAGAG TTCGCCCGTG CCGGACTGAA CGACCCAGTG 
CTCATTCGTT TAGACGTCGA ATCCAAGCTC CCGGATGCGC TGGCTCTAAA GTTTCTGTAC 
TGTCGACCAG ACGACCGCTA CACAGCACTT GTGGTTCTTC TTAAGTACGT TATTCCGGTG 
CAGTCGCAAA CCGTGGTGTT CGCCGGTACT CAGCACCATG TCGAACTCAT TTCGTACATA 
CTTACTGAGG CTGGAATCTC CAACGCCTCA GTCTACTCCA GCCTGGATCC AGCTGCACGC 
AAAATAAACA CCGCCAAATT TGTGAACAAA AAGGTGTCCG TGCTAATAGT CACGGACGTG 
GCCGCGCGTG GTATTGACAT TCCCAGTCTA GACTTTGTGG TGAACCTGCA CTTCCCGGGT 
AAACCGAAAC TGTTTGTCCA CCGGGTAGGT CGTTGCGCTC GCGCTGGTCG CACAGGAACC 
GCATACTCTA TTGTATCCAC AGACGACACA GCACATCTAC TGGATCTGCA CTTGTTCTTG 
AACAGACCCT TTAATATACA CGACAGCTCG GCATTGGGAA CCATTCCGCA AGATTTGCTC 
GAGGAAGAGC ACCTCACAGT GACCGATATA AAGAAAAGTC ACCATATTGC CGGAGTATTG 
CGCACCAGCG AAAACGCCTA CAAAAAATAC TTGAGTTCCC GACCAGTAGC CTCCACGGAT 
GCCAACGCGC GTGTAAAGAA AATTAAATTC TTTGCTCTCA AGCCATTGGA GGATTTCTTC 
ACAGCTGCCC CTGTGCTCGC CCAGGCGGCT GAAGCTAGTG GACAATCAAC AGAGTCGCAG 
GCAAAGGTGA GCGCCGCAGA GCGCAAGTTG CAGGAGGAAA AGCACGATAT ACTGGTGAAG 
ATGCGTAGTT TCCGTCCTGG TGGTACCGTT TTTGAACTCA ACACCACTCA AAAGTCCACG 
CAGTTTATCG TTATGAAGGA AAAACGAATG CAGCATGCGG AAGTAATCAA CAAGTTTAGG 
CAGCAGCGCG CAGAAGAGGA CATAGACGAG GAAAAGAAAA CGATCGAGGC GCTCAACCCA 
AGCTCAGGCA GAATGCCCAC CGCCGACGAG GAAGCTATTA GTAGTACTTT CAATAAAGTC 
GTTGCGCCCA AGAGGCTCCA GAACATGGAC GCCCTTTACA AGGACAAGCC AAAGAAGAAA 
AAGCGGAAGA TAAACAGCAA GGACGAGGAT CACTATGTGC CGTACCAGTC GGCAGACAAG 
CACACAGAGG ATGGTCTGGC CATTAACAAC TTTGAGCGGC AGGCGCAGAA CGCCGAGTTT 
TCGGTGTCGG ACCGCGACTT ATCCCAGGAA GTGAAGCACA AGCCCGGCCT TAAGAAGTGG 
GACCGAATTA AGAAAAAAAT GGTCTCTGTA CAAGATCCGC GAGCCAACAA AATTCGGACC 
GAGTCCGGTG CGTGGATTCC TGCCTCCTTT AAAACAGGTC GTTATGCTGA GTGGAAGGAG 
AAGTCTAAGA TCGAGGACCA GCTGCAGCGC GAAAATGCAG GTAGTGATGA CGAAAACGCC 
AAGCCGTTGA GCCATGCCCA GCGCTACCCA GTCAGTCGTC ATGCGCGGCA CAACTTAAAG 
CTAGAACTTA AGAAGCGACT GACCGGAAAC GACAAGGAGA TGCGCCGCCC CGAGCAAATT 
GTCAAGTCTC GCATGCGACT GGAGTTCATT AAAAAGCGCA ACGAAGAGAA CGCAGAGAGG 
AAAAGCGAAA ACCGCAAGCG CAGCATGCGG AAAACGCAGC GCCCCAAAGC TCAATCAACT 
GGGGGCTCGA AAAGAAGGAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_612028.4
CDS	66..2549
Translation	MRKKQADEIPGFPSLDNDAGTSDRGADILKSKSKKNKSGGFQSMGLGFELIKGITKRGYKVPTPIQRKTIPLILEGRDVVAMAKTGSGKTACFLIPLFEKLQRREPTKGARALILSPTRELAVQTYKFIKELGRFMELKSILVLGGDSMDSQFSAIHTCPDVIVATPGRFLHLCVEMDLKLNSIEYVVFDEADRLFEMGFGEQLNETLHRLPSSRQTVMFSATLPKLLVEFARAGLNDPVLIRLDVESKLPDALALKFLYCRPDDRYTALVVLLKYVIPVQSQTVVFAGTQHHVELISYILTEAGISNASVYSSLDPAARKINTAKFVNKKVSVLIVTDVAARGIDIPSLDFVVNLHFPGKPKLFVHRVGRCARAGRTGTAYSIVSTDDTAHLLDLHLFLNRPFNIHDSSALGTIPQDLLEEEHLTVTDIKKSHHIAGVLRTSENAYKKYLSSRPVASTDANARVKKIKFFALKPLEDFFTAAPVLAQAAEASGQSTESQAKVSAAERKLQEEKHDILVKMRSFRPGGTVFELNTTQKSTQFIVMKEKRMQHAEVINKFRQQRAEEDIDEEKKTIEALNPSSGRMPTADEEAISSTFNKVVAPKRLQNMDALYKDKPKKKKRKINSKDEDHYVPYQSADKHTEDGLAINNFERQAQNAEFSVSDRDLSQEVKHKPGLKKWDRIKKKMVSVQDPRANKIRTESGAWIPASFKTGRYAEWKEKSKIEDQLQRENAGSDDENAKPLSHAQRYPVSRHARHNLKLELKKRLTGNDKEMRRPEQIVKSRMRLEFIKKRNEENAERKSENRKRSMRKTQRPKAQSTGGSKRRK

Target ORF information:

RefSeq Version	NM_138184.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein (CG32344), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138184.5

ORF Insert Sequence:

ATGCGTAAGA AGCAGGCGGA TGAAATACCA GGCTTTCCAT CACTGGACAA CGATGCCGGC 
ACCAGCGACC GGGGCGCCGA CATTCTCAAG TCCAAGTCCA AGAAGAACAA GAGTGGTGGA 
TTCCAGTCGA TGGGCCTGGG CTTCGAGCTG ATAAAGGGCA TCACCAAGCG GGGTTACAAG 
GTTCCCACCC CCATACAGCG GAAAACGATT CCGCTGATCC TGGAGGGACG CGATGTGGTG 
GCAATGGCCA AGACGGGCTC TGGCAAGACG GCCTGCTTTC TGATTCCGCT CTTTGAAAAG 
CTACAGAGGC GCGAGCCCAC AAAGGGGGCC CGTGCCCTGA TCCTGTCTCC GACGCGCGAA 
CTGGCTGTGC AGACGTATAA GTTCATAAAG GAGCTGGGCC GCTTTATGGA ACTGAAGAGC 
ATTCTGGTGC TAGGTGGCGA CTCCATGGAC TCACAGTTCT CGGCCATACA TACCTGTCCG 
GACGTAATTG TTGCAACGCC AGGTCGTTTT CTTCATTTGT GCGTAGAAAT GGACTTGAAG 
CTAAATTCCA TAGAGTACGT GGTGTTTGAT GAAGCCGATC GACTCTTCGA GATGGGCTTC 
GGCGAGCAAC TGAACGAAAC GTTGCACAGA TTGCCATCGT CCCGGCAGAC GGTCATGTTT 
TCAGCCACGC TGCCAAAACT GCTAGTAGAG TTCGCCCGTG CCGGACTGAA CGACCCAGTG 
CTCATTCGTT TAGACGTCGA ATCCAAGCTC CCGGATGCGC TGGCTCTAAA GTTTCTGTAC 
TGTCGACCAG ACGACCGCTA CACAGCACTT GTGGTTCTTC TTAAGTACGT TATTCCGGTG 
CAGTCGCAAA CCGTGGTGTT CGCCGGTACT CAGCACCATG TCGAACTCAT TTCGTACATA 
CTTACTGAGG CTGGAATCTC CAACGCCTCA GTCTACTCCA GCCTGGATCC AGCTGCACGC 
AAAATAAACA CCGCCAAATT TGTGAACAAA AAGGTGTCCG TGCTAATAGT CACGGACGTG 
GCCGCGCGTG GTATTGACAT TCCCAGTCTA GACTTTGTGG TGAACCTGCA CTTCCCGGGT 
AAACCGAAAC TGTTTGTCCA CCGGGTAGGT CGTTGCGCTC GCGCTGGTCG CACAGGAACC 
GCATACTCTA TTGTATCCAC AGACGACACA GCACATCTAC TGGATCTGCA CTTGTTCTTG 
AACAGACCCT TTAATATACA CGACAGCTCG GCATTGGGAA CCATTCCGCA AGATTTGCTC 
GAGGAAGAGC ACCTCACAGT GACCGATATA AAGAAAAGTC ACCATATTGC CGGAGTATTG 
CGCACCAGCG AAAACGCCTA CAAAAAATAC TTGAGTTCCC GACCAGTAGC CTCCACGGAT 
GCCAACGCGC GTGTAAAGAA AATTAAATTC TTTGCTCTCA AGCCATTGGA GGATTTCTTC 
ACAGCTGCCC CTGTGCTCGC CCAGGCGGCT GAAGCTAGTG GACAATCAAC AGAGTCGCAG 
GCAAAGGTGA GCGCCGCAGA GCGCAAGTTG CAGGAGGAAA AGCACGATAT ACTGGTGAAG 
ATGCGTAGTT TCCGTCCTGG TGGTACCGTT TTTGAACTCA ACACCACTCA AAAGTCCACG 
CAGTTTATCG TTATGAAGGA AAAACGAATG CAGCATGCGG AAGTAATCAA CAAGTTTAGG 
CAGCAGCGCG CAGAAGAGGA CATAGACGAG GAAAAGAAAA CGATCGAGGC GCTCAACCCA 
AGCTCAGGCA GAATGCCCAC CGCCGACGAG GAAGCTATTA GTAGTACTTT CAATAAAGTC 
GTTGCGCCCA AGAGGCTCCA GAACATGGAC GCCCTTTACA AGGACAAGCC AAAGAAGAAA 
AAGCGGAAGA TAAACAGCAA GGACGAGGAT CACTATGTGC CGTACCAGTC GGCAGACAAG 
CACACAGAGG ATGGTCTGGC CATTAACAAC TTTGAGCGGC AGGCGCAGAA CGCCGAGTTT 
TCGGTGTCGG ACCGCGACTT ATCCCAGGAA GTGAAGCACA AGCCCGGCCT TAAGAAGTGG 
GACCGAATTA AGAAAAAAAT GGTCTCTGTA CAAGATCCGC GAGCCAACAA AATTCGGACC 
GAGTCCGGTG CGTGGATTCC TGCCTCCTTT AAAACAGGTC GTTATGCTGA GTGGAAGGAG 
AAGTCTAAGA TCGAGGACCA GCTGCAGCGC GAAAATGCAG GTAGTGATGA CGAAAACGCC 
AAGCCGTTGA GCCATGCCCA GCGCTACCCA GTCAGTCGTC ATGCGCGGCA CAACTTAAAG 
CTAGAACTTA AGAAGCGACT GACCGGAAAC GACAAGGAGA TGCGCCGCCC CGAGCAAATT 
GTCAAGTCTC GCATGCGACT GGAGTTCATT AAAAAGCGCA ACGAAGAGAA CGCAGAGAGG 
AAAAGCGAAA ACCGCAAGCG CAGCATGCGG AAAACGCAGC GCCCCAAAGC TCAATCAACT 
GGGGGCTCGA AAAGAAGGAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG32344 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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