Nepl3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nepl3
Entrez Gene ID	326172
Full Name	CG31918 gene product from transcript CG31918-RA
Synonyms	CG18592,Dmel\CG31918,anon-WO0118547.477
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 25C3-25C3

mRNA and Protein(s)

mRNA	Protein	Name
NM_135060.4	NP_608904.1	Neprilysin-like 3

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG4580	NP_609795.2
Drosophila melanogaster (fruit fly)	CG9505	NP_609019.1
Drosophila melanogaster (fruit fly)	CG9507	NP_609022.1
Drosophila melanogaster (fruit fly)	CG31918	NP_608904.1
Drosophila melanogaster (fruit fly)	CG8550	NP_610787.1
Drosophila melanogaster (fruit fly)	CG42370	NP_609023.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nepl3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nepl3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa15497	NM_135060.4 Latest version!	Drosophila melanogaster Neprilysin-like 3 (Nepl3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nepl3 ( NM_135060.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_608904.1 Drosophila melanogaster Neprilysin-like 3 (Nepl3), mRNA.

Price & Availability↑

CloneID	OFa15497
Clone ID Related Accession (Same CDS sequence)	NM_135060.4
Accession Version	NM_135060.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Neprilysin-like 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_135060.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGGAAT CCAGATCGTA CCTCAGCCTA AGCTGGCTGC TTCTTCTTCT GTGCAGTACA 
GTATGTTCTG CAAATCCCAA TCTCGAGATC ATGAAGCGAA TGCTAGACGA CATGGATGAA 
AAAGTCGAAC CATGTCACAA TTTCTACAAC TCCTCCCAAA AGAATCTCAA GTGGCATGCA 
TTTGCGACGG TGAGCGATCA GTTCCAACTC CTCTTCGACG GAATTGAGAA CGAGGTGTTC 
GAGAAGCACA GCTTCGAGGA AAAGTTGCAG CGTCTATACA AAATCTGTTC GACCATGGAG 
AGACCCAGTC TTCTGAGGTA TTTTGAGCTG GTGAAGCCGG GAGAAGATCT CAACTGGCCC 
TTTCTGACGC CCCGAGGAGG CAAGTGGCCC AAGGAGAAGT TTCAGTGGAT GGAGGCACTG 
GCTCGACTGC GTCGGTACGG CTTCAATGAC ATCATGTTTG GAATGAGTAT AAGACAGGAT 
CCGAACAGTG ACAGTGGCCA ACCCATAGTA GTTTTTGAAA AAAAGTACGA TTCTCTATTC 
CGTTTCATAG AGTCGCTGCA AAATATTGGT CTGCCACCAA AATTGGCAAA GGAGATAGCG 
CCTCTGGATA ATGCCATACA AGAAATGTAC AAATCAGATA TGAAATTTGA ACACTATGCA 
CGAGATGCAG CACCAGTGAA ACGGATTAGT CTAGGGCAGT TGGAAATTTA TGGGGTGTCC 
CTAACCAAGT ATCTGGAGAT CGTCTTCGGA CGCCCCTTTT CGCCGGATTT TGTAGTGCAT 
GTGGAGAATC TGTACTACTT ACGGGGGCTC CAGAATTTGA TGACTGCCGT TCCGAGCCAG 
GTGGTGGCCA ATTATCTAAC GGCACGCTTC AGCTTCTATA TAATGTTCAC GTTCATGAAG 
CATGGAGATT CATGCGTGAC TACAATGCGC CAATCCATGG AATTCGCCAG CAATCTGCTG 
TTCGAGGAGC GAATTCTGGG ACGCAAGAAG CTGCGTAGCT ATCAAAACCA GGCCGGCAAG 
ATATTTGATG CCATACGCCA GCAGCTAAAC ATCCGGCTCG AAAGGAACGT TCTTAAGCTG 
CCGCCCGCTG AGATTTCCTT ACTGCAGAAG AAGTTGAATG GCCTGAGCCT GAGCATCGGC 
AATCTGCCGG GAAAGGCGGG CCATCGACGT TTCGTAACCC GATACTACAA GGAGCTTGAA 
TTTGCGGACG ACGAGGAACT TGCGACTGCT CACCTAAAGG TTCTGGAGCA CCGAACGCGT 
GCCGAACTAA GCTTACTGGA CCAACCGAAC TTCAAAGACC GGCGATACAT GCTCCATTCA 
TATCCCATTT CGAATTTCTT CTTCGAAACT GCGTATGAGG AAAATGGAAA TACGATTTAT 
GTGGCATACG ACTTTCTGGC AGATGCTTCA TTTGATCCCG ACAGCCACGA TATCTTCAAA 
GTGACACCCT TGGGCATTCG CTTTGGCCTT GAGATGTTAA AAAGTTTCGA TCTTTTTAAT 
TGCAAACATC ATAAGGTCAA TATGCTCCAA TTGTTCGACG ATAACCAAAT TACTACCGCC 
GGCGATGAGT GTGTAAGCGC ATGGACCCAA GATAATTTGC TTAGCCAACG CGACCAGTAC 
CTCGTCGTGC TCCATTTGGT CCACGAAGCC TACTTCGCAG AAGGTTCCAA GTTCAGCCAG 
AGGCAGCCCA ACTTCACCAC CATGTCTCTG AGGGAATTGT TCTTCTTCAG ATTTGGCCAG 
AGTTCCTTCG GCCAAACCCT GTATTTCGGC AGCGATTTCG TCGAGGGCGG TTCACCCATA 
AATTCCCTGA CAAGGCTGCC TGTTTTTGTC CGGACCTTCA ACTGCTTGGC TCCAGGGGAG 
CAGCTCACCA CCACACAGAA ATCTACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_608904.1
CDS	113..2002
Translation	MMESRSYLSLSWLLLLLCSTVCSANPNLEIMKRMLDDMDEKVEPCHNFYNSSQKNLKWHAFATVSDQFQLLFDGIENEVFEKHSFEEKLQRLYKICSTMERPSLLRYFELVKPGEDLNWPFLTPRGGKWPKEKFQWMEALARLRRYGFNDIMFGMSIRQDPNSDSGQPIVVFEKKYDSLFRFIESLQNIGLPPKLAKEIAPLDNAIQEMYKSDMKFEHYARDAAPVKRISLGQLEIYGVSLTKYLEIVFGRPFSPDFVVHVENLYYLRGLQNLMTAVPSQVVANYLTARFSFYIMFTFMKHGDSCVTTMRQSMEFASNLLFEERILGRKKLRSYQNQAGKIFDAIRQQLNIRLERNVLKLPPAEISLLQKKLNGLSLSIGNLPGKAGHRRFVTRYYKELEFADDEELATAHLKVLEHRTRAELSLLDQPNFKDRRYMLHSYPISNFFFETAYEENGNTIYVAYDFLADASFDPDSHDIFKVTPLGIRFGLEMLKSFDLFNCKHHKVNMLQLFDDNQITTAGDECVSAWTQDNLLSQRDQYLVVLHLVHEAYFAEGSKFSQRQPNFTTMSLRELFFFRFGQSSFGQTLYFGSDFVEGGSPINSLTRLPVFVRTFNCLAPGEQLTTTQKST

Target ORF information:

RefSeq Version	NM_135060.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Neprilysin-like 3 (Nepl3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135060.4

ORF Insert Sequence:

ATGATGGAAT CCAGATCGTA CCTCAGCCTA AGCTGGCTGC TTCTTCTTCT GTGCAGTACA 
GTATGTTCTG CAAATCCCAA TCTCGAGATC ATGAAGCGAA TGCTAGACGA CATGGATGAA 
AAAGTCGAAC CATGTCACAA TTTCTACAAC TCCTCCCAAA AGAATCTCAA GTGGCATGCA 
TTTGCGACGG TGAGCGATCA GTTCCAACTC CTCTTCGACG GAATTGAGAA CGAGGTGTTC 
GAGAAGCACA GCTTCGAGGA AAAGTTGCAG CGTCTATACA AAATCTGTTC GACCATGGAG 
AGACCCAGTC TTCTGAGGTA TTTTGAGCTG GTGAAGCCGG GAGAAGATCT CAACTGGCCC 
TTTCTGACGC CCCGAGGAGG CAAGTGGCCC AAGGAGAAGT TTCAGTGGAT GGAGGCACTG 
GCTCGACTGC GTCGGTACGG CTTCAATGAC ATCATGTTTG GAATGAGTAT AAGACAGGAT 
CCGAACAGTG ACAGTGGCCA ACCCATAGTA GTTTTTGAAA AAAAGTACGA TTCTCTATTC 
CGTTTCATAG AGTCGCTGCA AAATATTGGT CTGCCACCAA AATTGGCAAA GGAGATAGCG 
CCTCTGGATA ATGCCATACA AGAAATGTAC AAATCAGATA TGAAATTTGA ACACTATGCA 
CGAGATGCAG CACCAGTGAA ACGGATTAGT CTAGGGCAGT TGGAAATTTA TGGGGTGTCC 
CTAACCAAGT ATCTGGAGAT CGTCTTCGGA CGCCCCTTTT CGCCGGATTT TGTAGTGCAT 
GTGGAGAATC TGTACTACTT ACGGGGGCTC CAGAATTTGA TGACTGCCGT TCCGAGCCAG 
GTGGTGGCCA ATTATCTAAC GGCACGCTTC AGCTTCTATA TAATGTTCAC GTTCATGAAG 
CATGGAGATT CATGCGTGAC TACAATGCGC CAATCCATGG AATTCGCCAG CAATCTGCTG 
TTCGAGGAGC GAATTCTGGG ACGCAAGAAG CTGCGTAGCT ATCAAAACCA GGCCGGCAAG 
ATATTTGATG CCATACGCCA GCAGCTAAAC ATCCGGCTCG AAAGGAACGT TCTTAAGCTG 
CCGCCCGCTG AGATTTCCTT ACTGCAGAAG AAGTTGAATG GCCTGAGCCT GAGCATCGGC 
AATCTGCCGG GAAAGGCGGG CCATCGACGT TTCGTAACCC GATACTACAA GGAGCTTGAA 
TTTGCGGACG ACGAGGAACT TGCGACTGCT CACCTAAAGG TTCTGGAGCA CCGAACGCGT 
GCCGAACTAA GCTTACTGGA CCAACCGAAC TTCAAAGACC GGCGATACAT GCTCCATTCA 
TATCCCATTT CGAATTTCTT CTTCGAAACT GCGTATGAGG AAAATGGAAA TACGATTTAT 
GTGGCATACG ACTTTCTGGC AGATGCTTCA TTTGATCCCG ACAGCCACGA TATCTTCAAA 
GTGACACCCT TGGGCATTCG CTTTGGCCTT GAGATGTTAA AAAGTTTCGA TCTTTTTAAT 
TGCAAACATC ATAAGGTCAA TATGCTCCAA TTGTTCGACG ATAACCAAAT TACTACCGCC 
GGCGATGAGT GTGTAAGCGC ATGGACCCAA GATAATTTGC TTAGCCAACG CGACCAGTAC 
CTCGTCGTGC TCCATTTGGT CCACGAAGCC TACTTCGCAG AAGGTTCCAA GTTCAGCCAG 
AGGCAGCCCA ACTTCACCAC CATGTCTCTG AGGGAATTGT TCTTCTTCAG ATTTGGCCAG 
AGTTCCTTCG GCCAAACCCT GTATTTCGGC AGCGATTTCG TCGAGGGCGG TTCACCCATA 
AATTCCCTGA CAAGGCTGCC TGTTTTTGTC CGGACCTTCA ACTGCTTGGC TCCAGGGGAG 
CAGCTCACCA CCACACAGAA ATCTACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Nepl3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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