tho2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	tho2
Entrez Gene ID	326153
Full Name	CG31671 gene product from transcript CG31671-RA
Synonyms	CG31671,CG4263,Dmel\CG31671,THO2,Tho2,macadamia
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 22D1-22D1

mRNA and Protein(s)

mRNA	Protein	Name
NM_164454.2	NP_722763.1	tho2, isoform A
NM_001272976.1	NP_001259905.1	tho2, isoform C
NM_134802.4	NP_608646.3	tho2, isoform B

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Thoc2	NP_001028594.1
Drosophila melanogaster (fruit fly)	tho2	NP_722763.1
Arabidopsis thaliana (thale cress)	THO2	NP_173871.6
Xenopus tropicalis (tropical clawed frog)	thoc2	XP_002931869.1
Bos taurus (cattle)	THOC2	NP_001193626.1
Rattus norvegicus (Norway rat)	Thoc2	NP_001178685.1
	THOC2	XP_001086217.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008236	XP_317233.4
Homo sapiens (human)	THOC2	NP_001075019.1
Canis lupus familiaris (dog)	THOC2	XP_005641829.1
Danio rerio (zebrafish)	thoc2	NP_001003847.1
Oryza sativa (rice)	Os01g0916800	NP_001045197.2
Pan troglodytes (chimpanzee)	THOC2	XP_001139291.2
Gallus gallus (chicken)	THOC2	XP_420332.2
Caenorhabditis elegans (roundworm)	thoc-2	NP_498392.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following tho2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tho2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa15477	NM_164454.2 Latest version!	Drosophila melanogaster tho2, transcript variant A (tho2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa15478	NM_134802.4 Latest version!	Drosophila melanogaster tho2, transcript variant B (tho2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa15990	NM_001272976.1 Latest version!	Drosophila melanogaster tho2, transcript variant C (tho2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tho2 ( NM_164454.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_722763.1 Drosophila melanogaster tho2, transcript variant A (tho2), mRNA.

Price & Availability↑

CloneID	OFa15477
Clone ID Related Accession (Same CDS sequence)	NM_164454.2
Accession Version	NM_164454.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tho2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164454.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGAGAT CCACAGACCG GTTGCGCCGG CTGAAAAAGC TGAAGAGCGT TACGGGAGCG 
CAGGATGGCG TTGGTGGCGG CGATGCGGCG GCGACGGCGG ATAATCGGAC CTCCGCATCG 
TCGGACACGA ACGGAGCAGC CGCCAGTAGC GGAGCCGGCG GCAATCAGCC AGGCACACTC 
AATCCGGAGG TCTTCAAGCA CTTGGACAAA TCCGGACGCA GTGAATTCCT GAAGTACCTC 
AAGCATCAGT TGCAGGATGC GGCGGATACG CCCGTTTACG ACAAGAACGG CGTCTTTAAG 
CAGGGCATCT CCAATGCCAT ATACCAGCTT CTTTGGCAGA CTCTGCGCTT CACTCACAAA 
AAAGACATAG TACTCCACCT GCTCCTGGAA GTCGTGGCCC TCCATGCGGA CTTTCCCAGC 
CTTATAGTGG ACGTGGTTAA CATTCTGGAC AGCGAAACCT CCCTTATAAC CGATGGACTT 
CAGGAAGAAC GACATGCCTT TGTGCAACTT GTTAAAGACT TGGACAGGGT GATTCCAGAA 
AGTCTTTTGA AAGAACGTTT GGAAATCGAT ACGTTGCAGG AGGCGGGAAT CGTCAAGAAC 
AAGAGCTTTT ACTCTAAGTT CATCAAGGTC AAAACCAAGC TATACTACAA GCAGCGTCGG 
TTTAACCTTT TCCGCGAGGA GAGTGAAGGG TTTGCCAAGC TTATCACAGA GCTAAACCAG 
GAGTTCGACG AGAACACCAC TCCAGAGAGC ATAATGGATA TCATAAAATC CCTTATAGGC 
TGCTTCAATC TGGATCCTAA TCGTGTGCTG GACATTATTA TCGAGTCCTT TGAAACTCGT 
CCGGATCGAT GGAACTTGTT CATACCCCTG CTGCGCAGCT ACATGCCCAC TGGTGCCATT 
ATTTGCGAAG TCCTCGGCTA CAAGTTCTGC CACTTTAAGG ACTCCCGCAC TCCCCGCTCG 
TTATATCATG TATGTGCTCT TTTGCTTAAG CATGGCGTCA TTGCTCTGAA CGATGTATAC 
GTGTGGTTGA CTCCAAACGA TGGGAGCATT AAGGCGGACT GGGAGGAAGA CCTGGCTGAT 
GCCAGGGAAA TGGTCCGCAA GCTGAACCTT ATACAAACCA ATAAGAAGGA AGACGAAAAG 
GATCCACCAC CGCCGCCTTC AGTTAAGAAG TTCAATGAGG AAAAATACAA TGCAAACCAA 
AAATTTGGCC TCTGCGAAGC TTTGCTTAAA GTTGGCGACT GGGAGAATGC GTACAAGATC 
ATCCAGAAGC TTCCCGAGCA GGCGGTTGTA TTGCAGGAAC CCATTGCCCG TGCCATTGCC 
GATCTGATTC ACCTGTCCGT GGAGAATATT TATTACAAAA AGTGTTTTAA GGCTCCCGCA 
GGACGTAGGC CGAGCCGCAA TCGCCTGTAT GAGGATTCCA AGTTGGTGGC CAAGATGCAG 
GCAAAGGAAT TCGGCGACTT AAGGAAGTAC ACCTGGCCGA TGGCTAACGT GTTGGGACCT 
GCCATGCACT ACGACACTGT GCTGATGTAC AAACTAATCC GTATTATGCG GAAACTAGTT 
GTCGATATGG GCGTGGACAG CCTAAATGGA CCTCCGCCGA ATTCGGAAGC AGAGCAGCAC 
TACTATGACA TTATGAGCTC GCTGGACGCC TGCATTCTAC CCTCCCTTCT ATACTTGGAC 
AACAACTGTT CCATGTCCGA AGAGATCTGG GCTGTGCTTA AATATTTCCC GTATCATTTT 
CGCTACAGTT TGTATGCGCG TTGGAAGAAC GACAGCTATC AGCTGCATCC CAATTTGATC 
AGGAGATGTG GATTGGCACA ACGGGATATT AAGGCCCTAA TGAAGCGCGT GAGCAAGGAG 
AACGTCAAGC AGCTGGGACG ACTGGTAGGA AAGTATAGCC ATTGTGCGCC AGGACTGCTT 
TTCGACTATA TTCTCTTGCA AATTCAAATC TATGATAATC TAATTGGTCC GGTCTGCGAC 
CTGCTCAAGT ATCTTACTGC CTTATCCTTT GACTGTTTGG GATACTGCAT CATCGAGTCG 
TTGACGTTAA CAGGTCGTCT CAGGTTCAAG GATGACGGCA CTTCGTTATC CTTGTGGCTG 
CAAAGTCTTG CCTCCTTCTG CGGCACCATA TACAAGAAGT ACAGCATTGA GCTAAGTGGT 
CTGCTTCAGT ATGTGGCCAA TCAGTTAAAG TCGCAGAAGA GCCTGGACTT ACTGATTCTG 
AGGGAGATCG TGCATAAGAT GGCAGGCGTG GAGTCCTGCG AGGAGATGAC TAATGATCAG 
TTGCAAGCCA TGTGTGGTGG TGAGCAGCTC AGGGGAGAGG CTGGCTACTT TAGTCAAGTG 
AGGAATACGA AAAAGTCCTC TAATCGCCTA AAGGAGGCAT TGGCCAATAA CGATCTTGCC 
GTGGCTATTT GCCTTTTGAT GGCTCAACAG AAACACTGCG TCATTTATCG TGAGACGGCG 
GCACACAGCC ATTTGAAGTT AGTGGGAAAT CTGTATGATC AGTGCCAAGA CACACTCGTC 
CAGTTTGGTA CGTTCCTAGG CTCCACCTAT TCTGTGGATG AGTATGTGGA GCGGTTGCCC 
TCGATTATAA CCATGCTTCG AGAGTACCAC ATAAACACGG ATGTGGCCTT CTTCTTGGCT 
AGGCCCATGT TTACTCATCA GATCAATCAA AAATATGATC AATTGCGCAA GGATGACCCT 
AACGCCAAGA AGCTCACCAC GACGCAAAAG CTCCAGAAGT ATTTGGAAGC TACGCAGCTG 
ATCATGAACC CTATTGTAGA GTCTGTTCGT CCACTGCACA GCTCCAAGGT GTGGGAGGAC 
ATTAGTCCGC AGTTCCTGGT AACTTTTTGG AGTCTGAGCA TGTACGATCT GCACGTGCCG 
AACGAAAGCT ATCAGAGGGA GGTTGCCAAG CTAAAGCAGC TGGCCCAGCA AGCGGCAGAA 
GGCAAAGACT CTAACCAATC TAAGAACAAG AAGGAGCAGG AGCGCTACAT TGCGCTAATG 
GAGAAGCTGA ACGACGAGCG AAAGAAGCAG CATGAGCACG TTGACAAGAT CTCGCAGCGT 
TTGCAGGAGC AAAAAGATAG TTGGTTCCTT CTACGATCTG GCAAGTCCGC AAAGAACGAC 
ACCATCACGC AATTCCTGCA GCTCTGTCTC TTCCCGCGTT GCACCTTCAC AGCTCTGGAT 
GCGTTGTATT GCGCGAAGTT CGTGCACACC ATCCACAATC TCAAGACATC GAACTTCTCA 
ACCTTGCTGT GCTATGATAG GATCTTTTGT GACATCACTT ATTCGGTAAC ATCGTGCACT 
GAGGGCGAGG CTACGCGGTA CGGACGCTTT TTGTGCGCCA TGCTCGAGAC CGTGATGCGT 
TGGCATGCGG ATCAGGCGGT GTTTAATAAG GAGTGCGCCA ATTATCCTGG TTTTGTGACA 
AAGTTTCGTG TTAGCAATCA GTTCTCAGAG GCCAACGATC ATGTTGGCTA CGAAAACTAT 
CGGCATGTGT GTCACAAGTG GCACTACAAG ATTACCAAGG CGATTGTATT CTGTCTGGAC 
TCGAAGGACT TTATGCAGAT CAGAAACGCA CTGATCATCC TAATGCGAAT ACTGCCTCAC 
TATCCAGTTC TTTCCAAACT GGCGCAAATC ATCGAGCGTA AGGTGGACAA GGTGCGCGAG 
GAGGAGAAGA CAAAGCGACC GGATCTGTAT GCCATTGCCT CCAGCTACAT TGGACAACTA 
AAGCTGAAGA CTCCCCACAT GCTAAAGGAG AGTGTCTTTC ATCAAATCGC CGAGCGGCCG 
AATAAGGAGT CGCCGACCAG TGTTGGCGCT CCCGCCGCAG CCACCAGATC CGATAAGTTG 
TCACCCACCT CTCCATCTGG GAATACACAA GGAACAAGGG CTCCAGGCGG AGCTGCTCCT 
TTCTACAACA GCGAACAAAA GTCGGTGATC AAAGAACCAG ATGCAAAAGC AGCCTCCACA 
ACGCGTGAAT CGAAGAGCCA AAGAGGCGAG GGCAATAATG TCACTTTGGT GTCCACAGCC 
ACTTCAACGG CATCCAATAA CGAACGCGAG AGCAAACAAC GTGACCTGCC AGCGCCACGT 
GAGTCCCAAT CCCGCAGCAA GGATGACCAG CAGGAGCAGG CAAACAACGG CAGTAATGGC 
AGCCGACAGA GCGAGAGTCG TAATAGAGAT GTGGAACGAG ACCGACATGA TCAGCAAGAC 
CAACGAAGCA TTAGTAGCCA TCGCAGTTCC AGAGACATTG TGCGGGTCAA GGAGCGCACA 
GAAGCCGAGC TGCATCAGAG GTCGCGGGAA CGTTCTCAAC GTCTGGAGGA GTTGGAAGCG 
CAGCAGCGGA AGCGGGAAAA AACATCGCGA CGGGGCGGCG AAGAGCGCAT CCGACATGGT 
GATGGCGTCG AGACAGTGGA CCTGGTGGGC AGCACCGACA ATCGCCACTA CGAGGAGTTC 
GAGGGGCGTA TGAGGGACCT GAGCTCAGTG TCCAACGAGA GCAACGGTTC GTTGCACCAT 
CGACAACGTA GCCATGAGAC CATTGAATTT GAAAAGGATT CAAAGCGTCG CAAATTGGAG 
TCATCAACCA GCAGTTCAAA GAAGGTGGAA GAACTGGTGG ACAGCGTAAA GAAGGCGCGC 
GCCCTTAAGA CCAAAGAGCG CAACAAGGAC AAACTGTCGG ATGAGGAGCG AGACGCTCGC 
AAGGATCGCA AGCTGGGACG CAAGCGGGAT CGCGTCGACG AGTCCAACAG CAACGAACAT 
AAACGCCGGC GCGAAGGACA AAACGGTGAA GAAGAGCTGC GGGATAGAGA GCGGCATAGG 
GAGAAGTCAC CGCGGGAGCG ATCACATGAA AAGTTCGATA GAGAAAGAGG AGGAGCCAGC 
GGTTCGCGGG CCGAGGAGCG GCAGTACATC AGCAAGTCCG CTCGGTCCTC CAGAGTCAAC 
TATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_722763.1
CDS	157..5082
Translation	MQRSTDRLRRLKKLKSVTGAQDGVGGGDAAATADNRTSASSDTNGAAASSGAGGNQPGTLNPEVFKHLDKSGRSEFLKYLKHQLQDAADTPVYDKNGVFKQGISNAIYQLLWQTLRFTHKKDIVLHLLLEVVALHADFPSLIVDVVNILDSETSLITDGLQEERHAFVQLVKDLDRVIPESLLKERLEIDTLQEAGIVKNKSFYSKFIKVKTKLYYKQRRFNLFREESEGFAKLITELNQEFDENTTPESIMDIIKSLIGCFNLDPNRVLDIIIESFETRPDRWNLFIPLLRSYMPTGAIICEVLGYKFCHFKDSRTPRSLYHVCALLLKHGVIALNDVYVWLTPNDGSIKADWEEDLADAREMVRKLNLIQTNKKEDEKDPPPPPSVKKFNEEKYNANQKFGLCEALLKVGDWENAYKIIQKLPEQAVVLQEPIARAIADLIHLSVENIYYKKCFKAPAGRRPSRNRLYEDSKLVAKMQAKEFGDLRKYTWPMANVLGPAMHYDTVLMYKLIRIMRKLVVDMGVDSLNGPPPNSEAEQHYYDIMSSLDACILPSLLYLDNNCSMSEEIWAVLKYFPYHFRYSLYARWKNDSYQLHPNLIRRCGLAQRDIKALMKRVSKENVKQLGRLVGKYSHCAPGLLFDYILLQIQIYDNLIGPVCDLLKYLTALSFDCLGYCIIESLTLTGRLRFKDDGTSLSLWLQSLASFCGTIYKKYSIELSGLLQYVANQLKSQKSLDLLILREIVHKMAGVESCEEMTNDQLQAMCGGEQLRGEAGYFSQVRNTKKSSNRLKEALANNDLAVAICLLMAQQKHCVIYRETAAHSHLKLVGNLYDQCQDTLVQFGTFLGSTYSVDEYVERLPSIITMLREYHINTDVAFFLARPMFTHQINQKYDQLRKDDPNAKKLTTTQKLQKYLEATQLIMNPIVESVRPLHSSKVWEDISPQFLVTFWSLSMYDLHVPNESYQREVAKLKQLAQQAAEGKDSNQSKNKKEQERYIALMEKLNDERKKQHEHVDKISQRLQEQKDSWFLLRSGKSAKNDTITQFLQLCLFPRCTFTALDALYCAKFVHTIHNLKTSNFSTLLCYDRIFCDITYSVTSCTEGEATRYGRFLCAMLETVMRWHADQAVFNKECANYPGFVTKFRVSNQFSEANDHVGYENYRHVCHKWHYKITKAIVFCLDSKDFMQIRNALIILMRILPHYPVLSKLAQIIERKVDKVREEEKTKRPDLYAIASSYIGQLKLKTPHMLKESVFHQIAERPNKESPTSVGAPAAATRSDKLSPTSPSGNTQGTRAPGGAAPFYNSEQKSVIKEPDAKAASTTRESKSQRGEGNNVTLVSTATSTASNNERESKQRDLPAPRESQSRSKDDQQEQANNGSNGSRQSESRNRDVERDRHDQQDQRSISSHRSSRDIVRVKERTEAELHQRSRERSQRLEELEAQQRKREKTSRRGGEERIRHGDGVETVDLVGSTDNRHYEEFEGRMRDLSSVSNESNGSLHHRQRSHETIEFEKDSKRRKLESSTSSSKKVEELVDSVKKARALKTKERNKDKLSDEERDARKDRKLGRKRDRVDESNSNEHKRRREGQNGEEELRDRERHREKSPRERSHEKFDRERGGASGSRAEERQYISKSARSSRVNY

Target ORF information:

RefSeq Version	NM_164454.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tho2, transcript variant A (tho2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164454.2

ORF Insert Sequence:

ATGCAGAGAT CCACAGACCG GTTGCGCCGG CTGAAAAAGC TGAAGAGCGT TACGGGAGCG 
CAGGATGGCG TTGGTGGCGG CGATGCGGCG GCGACGGCGG ATAATCGGAC CTCCGCATCG 
TCGGACACGA ACGGAGCAGC CGCCAGTAGC GGAGCCGGCG GCAATCAGCC AGGCACACTC 
AATCCGGAGG TCTTCAAGCA CTTGGACAAA TCCGGACGCA GTGAATTCCT GAAGTACCTC 
AAGCATCAGT TGCAGGATGC GGCGGATACG CCCGTTTACG ACAAGAACGG CGTCTTTAAG 
CAGGGCATCT CCAATGCCAT ATACCAGCTT CTTTGGCAGA CTCTGCGCTT CACTCACAAA 
AAAGACATAG TACTCCACCT GCTCCTGGAA GTCGTGGCCC TCCATGCGGA CTTTCCCAGC 
CTTATAGTGG ACGTGGTTAA CATTCTGGAC AGCGAAACCT CCCTTATAAC CGATGGACTT 
CAGGAAGAAC GACATGCCTT TGTGCAACTT GTTAAAGACT TGGACAGGGT GATTCCAGAA 
AGTCTTTTGA AAGAACGTTT GGAAATCGAT ACGTTGCAGG AGGCGGGAAT CGTCAAGAAC 
AAGAGCTTTT ACTCTAAGTT CATCAAGGTC AAAACCAAGC TATACTACAA GCAGCGTCGG 
TTTAACCTTT TCCGCGAGGA GAGTGAAGGG TTTGCCAAGC TTATCACAGA GCTAAACCAG 
GAGTTCGACG AGAACACCAC TCCAGAGAGC ATAATGGATA TCATAAAATC CCTTATAGGC 
TGCTTCAATC TGGATCCTAA TCGTGTGCTG GACATTATTA TCGAGTCCTT TGAAACTCGT 
CCGGATCGAT GGAACTTGTT CATACCCCTG CTGCGCAGCT ACATGCCCAC TGGTGCCATT 
ATTTGCGAAG TCCTCGGCTA CAAGTTCTGC CACTTTAAGG ACTCCCGCAC TCCCCGCTCG 
TTATATCATG TATGTGCTCT TTTGCTTAAG CATGGCGTCA TTGCTCTGAA CGATGTATAC 
GTGTGGTTGA CTCCAAACGA TGGGAGCATT AAGGCGGACT GGGAGGAAGA CCTGGCTGAT 
GCCAGGGAAA TGGTCCGCAA GCTGAACCTT ATACAAACCA ATAAGAAGGA AGACGAAAAG 
GATCCACCAC CGCCGCCTTC AGTTAAGAAG TTCAATGAGG AAAAATACAA TGCAAACCAA 
AAATTTGGCC TCTGCGAAGC TTTGCTTAAA GTTGGCGACT GGGAGAATGC GTACAAGATC 
ATCCAGAAGC TTCCCGAGCA GGCGGTTGTA TTGCAGGAAC CCATTGCCCG TGCCATTGCC 
GATCTGATTC ACCTGTCCGT GGAGAATATT TATTACAAAA AGTGTTTTAA GGCTCCCGCA 
GGACGTAGGC CGAGCCGCAA TCGCCTGTAT GAGGATTCCA AGTTGGTGGC CAAGATGCAG 
GCAAAGGAAT TCGGCGACTT AAGGAAGTAC ACCTGGCCGA TGGCTAACGT GTTGGGACCT 
GCCATGCACT ACGACACTGT GCTGATGTAC AAACTAATCC GTATTATGCG GAAACTAGTT 
GTCGATATGG GCGTGGACAG CCTAAATGGA CCTCCGCCGA ATTCGGAAGC AGAGCAGCAC 
TACTATGACA TTATGAGCTC GCTGGACGCC TGCATTCTAC CCTCCCTTCT ATACTTGGAC 
AACAACTGTT CCATGTCCGA AGAGATCTGG GCTGTGCTTA AATATTTCCC GTATCATTTT 
CGCTACAGTT TGTATGCGCG TTGGAAGAAC GACAGCTATC AGCTGCATCC CAATTTGATC 
AGGAGATGTG GATTGGCACA ACGGGATATT AAGGCCCTAA TGAAGCGCGT GAGCAAGGAG 
AACGTCAAGC AGCTGGGACG ACTGGTAGGA AAGTATAGCC ATTGTGCGCC AGGACTGCTT 
TTCGACTATA TTCTCTTGCA AATTCAAATC TATGATAATC TAATTGGTCC GGTCTGCGAC 
CTGCTCAAGT ATCTTACTGC CTTATCCTTT GACTGTTTGG GATACTGCAT CATCGAGTCG 
TTGACGTTAA CAGGTCGTCT CAGGTTCAAG GATGACGGCA CTTCGTTATC CTTGTGGCTG 
CAAAGTCTTG CCTCCTTCTG CGGCACCATA TACAAGAAGT ACAGCATTGA GCTAAGTGGT 
CTGCTTCAGT ATGTGGCCAA TCAGTTAAAG TCGCAGAAGA GCCTGGACTT ACTGATTCTG 
AGGGAGATCG TGCATAAGAT GGCAGGCGTG GAGTCCTGCG AGGAGATGAC TAATGATCAG 
TTGCAAGCCA TGTGTGGTGG TGAGCAGCTC AGGGGAGAGG CTGGCTACTT TAGTCAAGTG 
AGGAATACGA AAAAGTCCTC TAATCGCCTA AAGGAGGCAT TGGCCAATAA CGATCTTGCC 
GTGGCTATTT GCCTTTTGAT GGCTCAACAG AAACACTGCG TCATTTATCG TGAGACGGCG 
GCACACAGCC ATTTGAAGTT AGTGGGAAAT CTGTATGATC AGTGCCAAGA CACACTCGTC 
CAGTTTGGTA CGTTCCTAGG CTCCACCTAT TCTGTGGATG AGTATGTGGA GCGGTTGCCC 
TCGATTATAA CCATGCTTCG AGAGTACCAC ATAAACACGG ATGTGGCCTT CTTCTTGGCT 
AGGCCCATGT TTACTCATCA GATCAATCAA AAATATGATC AATTGCGCAA GGATGACCCT 
AACGCCAAGA AGCTCACCAC GACGCAAAAG CTCCAGAAGT ATTTGGAAGC TACGCAGCTG 
ATCATGAACC CTATTGTAGA GTCTGTTCGT CCACTGCACA GCTCCAAGGT GTGGGAGGAC 
ATTAGTCCGC AGTTCCTGGT AACTTTTTGG AGTCTGAGCA TGTACGATCT GCACGTGCCG 
AACGAAAGCT ATCAGAGGGA GGTTGCCAAG CTAAAGCAGC TGGCCCAGCA AGCGGCAGAA 
GGCAAAGACT CTAACCAATC TAAGAACAAG AAGGAGCAGG AGCGCTACAT TGCGCTAATG 
GAGAAGCTGA ACGACGAGCG AAAGAAGCAG CATGAGCACG TTGACAAGAT CTCGCAGCGT 
TTGCAGGAGC AAAAAGATAG TTGGTTCCTT CTACGATCTG GCAAGTCCGC AAAGAACGAC 
ACCATCACGC AATTCCTGCA GCTCTGTCTC TTCCCGCGTT GCACCTTCAC AGCTCTGGAT 
GCGTTGTATT GCGCGAAGTT CGTGCACACC ATCCACAATC TCAAGACATC GAACTTCTCA 
ACCTTGCTGT GCTATGATAG GATCTTTTGT GACATCACTT ATTCGGTAAC ATCGTGCACT 
GAGGGCGAGG CTACGCGGTA CGGACGCTTT TTGTGCGCCA TGCTCGAGAC CGTGATGCGT 
TGGCATGCGG ATCAGGCGGT GTTTAATAAG GAGTGCGCCA ATTATCCTGG TTTTGTGACA 
AAGTTTCGTG TTAGCAATCA GTTCTCAGAG GCCAACGATC ATGTTGGCTA CGAAAACTAT 
CGGCATGTGT GTCACAAGTG GCACTACAAG ATTACCAAGG CGATTGTATT CTGTCTGGAC 
TCGAAGGACT TTATGCAGAT CAGAAACGCA CTGATCATCC TAATGCGAAT ACTGCCTCAC 
TATCCAGTTC TTTCCAAACT GGCGCAAATC ATCGAGCGTA AGGTGGACAA GGTGCGCGAG 
GAGGAGAAGA CAAAGCGACC GGATCTGTAT GCCATTGCCT CCAGCTACAT TGGACAACTA 
AAGCTGAAGA CTCCCCACAT GCTAAAGGAG AGTGTCTTTC ATCAAATCGC CGAGCGGCCG 
AATAAGGAGT CGCCGACCAG TGTTGGCGCT CCCGCCGCAG CCACCAGATC CGATAAGTTG 
TCACCCACCT CTCCATCTGG GAATACACAA GGAACAAGGG CTCCAGGCGG AGCTGCTCCT 
TTCTACAACA GCGAACAAAA GTCGGTGATC AAAGAACCAG ATGCAAAAGC AGCCTCCACA 
ACGCGTGAAT CGAAGAGCCA AAGAGGCGAG GGCAATAATG TCACTTTGGT GTCCACAGCC 
ACTTCAACGG CATCCAATAA CGAACGCGAG AGCAAACAAC GTGACCTGCC AGCGCCACGT 
GAGTCCCAAT CCCGCAGCAA GGATGACCAG CAGGAGCAGG CAAACAACGG CAGTAATGGC 
AGCCGACAGA GCGAGAGTCG TAATAGAGAT GTGGAACGAG ACCGACATGA TCAGCAAGAC 
CAACGAAGCA TTAGTAGCCA TCGCAGTTCC AGAGACATTG TGCGGGTCAA GGAGCGCACA 
GAAGCCGAGC TGCATCAGAG GTCGCGGGAA CGTTCTCAAC GTCTGGAGGA GTTGGAAGCG 
CAGCAGCGGA AGCGGGAAAA AACATCGCGA CGGGGCGGCG AAGAGCGCAT CCGACATGGT 
GATGGCGTCG AGACAGTGGA CCTGGTGGGC AGCACCGACA ATCGCCACTA CGAGGAGTTC 
GAGGGGCGTA TGAGGGACCT GAGCTCAGTG TCCAACGAGA GCAACGGTTC GTTGCACCAT 
CGACAACGTA GCCATGAGAC CATTGAATTT GAAAAGGATT CAAAGCGTCG CAAATTGGAG 
TCATCAACCA GCAGTTCAAA GAAGGTGGAA GAACTGGTGG ACAGCGTAAA GAAGGCGCGC 
GCCCTTAAGA CCAAAGAGCG CAACAAGGAC AAACTGTCGG ATGAGGAGCG AGACGCTCGC 
AAGGATCGCA AGCTGGGACG CAAGCGGGAT CGCGTCGACG AGTCCAACAG CAACGAACAT 
AAACGCCGGC GCGAAGGACA AAACGGTGAA GAAGAGCTGC GGGATAGAGA GCGGCATAGG 
GAGAAGTCAC CGCGGGAGCG ATCACATGAA AAGTTCGATA GAGAAAGAGG AGGAGCCAGC 
GGTTCGCGGG CCGAGGAGCG GCAGTACATC AGCAAGTCCG CTCGGTCCTC CAGAGTCAAC 
TATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tho2 ( NM_134802.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_608646.3 Drosophila melanogaster tho2, transcript variant B (tho2), mRNA.

Price & Availability↑

CloneID	OFa15478
Clone ID Related Accession (Same CDS sequence)	NM_134802.4
Accession Version	NM_134802.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tho2, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_134802.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGAGAT CCACAGACCG GTTGCGCCGG CTGAAAAAGC TGAAGAGCGT TACGGGAGCG 
CAGGATGGCG TTGGTGGCGG CGATGCGGCG GCGACGGCGG ATAATCGGAC CTCCGCATCG 
TCGGACACGA ACGGAGCAGC CGCCAGTAGC GGAGCCGGCG GCAATCAGCC AGGCACACTC 
AATCCGGAGG TCTTCAAGCA CTTGGACAAA TCCGGACGCA GTGAATTCCT GAAGTACCTC 
AAGCATCAGT TGCAGGATGC GGCGGATACG CCCGTTTACG ACAAGAACGG CGTCTTTAAG 
CAGGGCATCT CCAATGCCAT ATACCAGCTT CTTTGGCAGA CTCTGCGCTT CACTCACAAA 
AAAGACATAG TACTCCACCT GCTCCTGGAA GTCGTGGCCC TCCATGCGGA CTTTCCCAGC 
CTTATAGTGG ACGTGGTTAA CATTCTGGAC AGCGAAACCT CCCTTATAAC CGATGGACTT 
CAGGAAGAAC GACATGCCTT TGTGCAACTT GTTAAAGACT TGGACAGGGT GATTCCAGAA 
AGTCTTTTGA AAGAACGTTT GGAAATCGAT ACGTTGCAGG AGGCGGGAAT CGTCAAGAAC 
AAGAGCTTTT ACTCTAAGTT CATCAAGGTC AAAACCAAGC TATACTACAA GCAGCGTCGG 
TTTAACCTTT TCCGCGAGGA GAGTGAAGGG TTTGCCAAGC TTATCACAGA GCTAAACCAG 
GAGTTCGACG AGAACACCAC TCCAGAGAGC ATAATGGATA TCATAAAATC CCTTATAGGC 
TGCTTCAATC TGGATCCTAA TCGTGTGCTG GACATTATTA TCGAGTCCTT TGAAACTCGT 
CCGGATCGAT GGAACTTGTT CATACCCCTG CTGCGCAGCT ACATGCCCAC TGGTGCCATT 
ATTTGCGAAG TCCTCGGCTA CAAGTTCTGC CACTTTAAGG ACTCCCGCAC TCCCCGCTCG 
TTATATCATG TATGTGCTCT TTTGCTTAAG CATGGCGTCA TTGCTCTGAA CGATGTATAC 
GTGTGGTTGA CTCCAAACGA TGGGAGCATT AAGGCGGACT GGGAGGAAGA CCTGGCTGAT 
GCCAGGGAAA TGGTCCGCAA GCTGAACCTT ATACAAACCA ATAAGAAGGA AGACGAAAAG 
GATCCACCAC CGCCGCCTTC AGTTAAGAAG TTCAATGAGG AAAAATACAA TGCAAACCAA 
AAATTTGGCC TCTGCGAAGC TTTGCTTAAA GTTGGCGACT GGGAGAATGC GTACAAGATC 
ATCCAGAAGC TTCCCGAGCA GGCGGTTGTA TTGCAGGAAC CCATTGCCCG TGCCATTGCC 
GATCTGATTC ACCTGTCCGT GGAGAATATT TATTACAAAA AGTGTTTTAA GGCTCCCGCA 
GGACGTAGGC CGAGCCGCAA TCGCCTGTAT GAGGATTCCA AGTTGGTGGC CAAGATGCAG 
GCAAAGGAAT TCGGCGACTT AAGGAAGTAC ACCTGGCCGA TGGCTAACGT GTTGGGACCT 
GCCATGCACT ACGACACTGT GCTGATGTAC AAACTAATCC GTATTATGCG GAAACTAGTT 
GTCGATATGG GCGTGGACAG CCTAAATGGA CCTCCGCCGA ATTCGGAAGC AGAGCAGCAC 
TACTATGACA TTATGAGCTC GCTGGACGCC TGCATTCTAC CCTCCCTTCT ATACTTGGAC 
AACAACTGTT CCATGTCCGA AGAGATCTGG GCTGTGCTTA AATATTTCCC GTATCATTTT 
CGCTACAGTT TGTATGCGCG TTGGAAGAAC GACAGCTATC AGCTGCATCC CAATTTGATC 
AGGAGATGTG GATTGGCACA ACGGGATATT AAGGCCCTAA TGAAGCGCGT GAGCAAGGAG 
AACGTCAAGC AGCTGGGACG ACTGGTAGGA AAGTATAGCC ATTGTGCGCC AGGACTGCTT 
TTCGACTATA TTCTCTTGCA AATTCAAATC TATGATAATC TAATTGGTCC GGTCTGCGAC 
CTGCTCAAGT ATCTTACTGC CTTATCCTTT GACTGTTTGG GATACTGCAT CATCGAGTCG 
TTGACGTTAA CAGGTCGTCT CAGGTTCAAG GATGACGGCA CTTCGTTATC CTTGTGGCTG 
CAAAGTCTTG CCTCCTTCTG CGGCACCATA TACAAGAAGT ACAGCATTGA GCTAAGTGGT 
CTGCTTCAGT ATGTGGCCAA TCAGTTAAAG TCGCAGAAGA GCCTGGACTT ACTGATTCTG 
AGGGAGATCG TGCATAAGAT GGCAGGCGTG GAGTCCTGCG AGGAGATGAC TAATGATCAG 
TTGCAAGCCA TGTGTGGTGG TGAGCAGCTC AGGGGAGAGG CTGGCTACTT TAGTCAAGTG 
AGGAATACGA AAAAGTCCTC TAATCGCCTA AAGGAGGCAT TGGCCAATAA CGATCTTGCC 
GTGGCTATTT GCCTTTTGAT GGCTCAACAG AAACACTGCG TCATTTATCG TGAGACGGCG 
GCACACAGCC ATTTGAAGTT AGTGGGAAAT CTGTATGATC AGTGCCAAGA CACACTCGTC 
CAGTTTGGTA CGTTCCTAGG CTCCACCTAT TCTGTGGATG AGTATGTGGA GCGGTTGCCC 
TCGATTATAA CCATGCTTCG AGAGTACCAC ATAAACACGG ATGTGGCCTT CTTCTTGGCT 
AGGCCCATGT TTACTCATCA GATCAATCAA AAATATGATC AATTGCGCAA GGATGACCCT 
AACGCCAAGA AGCTCACCAC GACGCAAAAG CTCCAGAAGT ATTTGGAAGC TACGCAGCTG 
ATCATGAACC CTATTGTAGA GTCTGTTCGT CCACTGCACA GCTCCAAGGT GTGGGAGGAC 
ATTAGTCCGC AGTTCCTGGT AACTTTTTGG AGTCTGAGCA TGTACGATCT GCACGTGCCG 
AACGAAAGCT ATCAGAGGGA GGTTGCCAAG CTAAAGCAGC TGGCCCAGCA AGCGGCAGAA 
GGCAAAGACT CTAACCAATC TAAGAACAAG AAGGAGCAGG AGCGCTACAT TGCGCTAATG 
GAGAAGCTGA ACGACGAGCG AAAGAAGCAG CATGAGCACG TTGACAAGAT CTCGCAGCGT 
TTGCAGGAGC AAAAAGATAG TTGGTTCCTT CTACGATCTG GCAAGTCCGC AAAGAACGAC 
ACCATCACGC AATTCCTGCA GCTCTGTCTC TTCCCGCGTT GCACCTTCAC AGCTCTGGAT 
GCGTTGTATT GCGCGAAGTT CGTGCACACC ATCCACAATC TCAAGACATC GAACTTCTCA 
ACCTTGCTGT GCTATGATAG GATCTTTTGT GACATCACTT ATTCGGTAAC ATCGTGCACT 
GAGGGCGAGG CTACGCGGTA CGGACGCTTT TTGTGCGCCA TGCTCGAGAC CGTGATGCGT 
TGGCATGCGG ATCAGGCGGT GTTTAATAAG GAGTGCGCCA ATTATCCTGG TTTTGTGACA 
AAGTTTCGTG TTAGCAATCA GTTCTCAGAG GCCAACGATC ATGTTGGCTA CGAAAACTAT 
CGGCATGTGT GTCACAAGTG GCACTACAAG ATTACCAAGG CGATTGTATT CTGTCTGGAC 
TCGAAGGACT TTATGCAGAT CAGAAACGCA CTGATCATCC TAATGCGAAT ACTGCCTCAC 
TATCCAGTTC TTTCCAAACT GGCGCAAATC ATCGAGCGTA AGGTGGACAA GGTGCGCGAG 
GAGGAGAAGA CAAAGCGACC GGATCTGTAT GCCATTGCCT CCAGCTACAT TGGACAACTA 
AAGCTGAAGA CTCCCCACAT GCTAAAGGAG AGTGTCTTTC ATCAAATCGC CGAGCGGCCG 
AATAAGGAGT CGCCGACCAG TGTTGGCGCT CCCGCCGCAG CCACCAGATC CGATAAGTTG 
TCACCCACCT CTCCATCTGG GAATACACAA GGAACAAGGG CTCCAGGCGG AGCTGCTCCT 
TTCTACAACA GCGAACAAAA GTCGGTGATC AAAGAACCAG ATGCAAAAGC AGCCTCCACA 
ACGCGTGAAT CGAAGAGCCA AAGAGGCGAG GGCAATAATG TCACTTTGGT GTCCACAGCC 
ACTTCAACGG CATCCAATAA CGAACGCGAG AGCAAACAAC GTGACCTGCC AGCGCCACGT 
GAGTCCCAAT CCCGCAGCAA GGATGACCAG CAGGAGCAGG CAAACAACGG CAGTAATGGC 
AGCCGACAGA GCGAGAGTCG TAATAGAGAT GTGGAACGAG ACCGACATGA TCAGCAAGAC 
CAACGAAGCA TTAGTAGCCA TCGCAGTTCC AGAGACATTG TGCGGGTCAA GGAGCGCACA 
GAAGCCGAGC TGCATCAGAG GTCGCGGGAA CGTTCTCAAC GTCTGGAGGA GTTGGAAGCG 
CAGCAGCGGA AGCGGGAAAA AACATCGCGA CGGGGCGGCG AAGAGCGCAT CCGACATGGT 
GATGGCGTCG AGACAGTGGA CCTGGTGGGC AGCACCGACA ATCGCCACTA CGAGGAGTTC 
GAGGGGCGTA TGAGGGACCT GAGCTCAGTG TCCAACGAGA GCAACGGTTC GTTGCACCAT 
CGACAACGTA GCCATGAGAC CATTGAATTT GAAAAGGTAG ATTCAAAGCG TCGCAAATTG 
GAGTCATCAA CCAGCAGTTC AAAGAAGGTG GAAGAACTGG TGGACAGCGT AAAGAAGGCG 
CGCGCCCTTA AGACCAAAGA GCGCAACAAG GACAAACTGT CGGATGAGGA GCGAGACGCT 
CGCAAGGATC GCAAGCTGGG ACGCAAGCGG GATCGCGTCG ACGAGTCCAA CAGCAACGAA 
CATAAACGCC GGCGCGAAGG ACAAAACGGT GAAGAAGAGC TGCGGGATAG AGAGCGGCAT 
AGGGAGAAGT CACCGCGGGA GCGATCACAT GAAAAGTTCG ATAGAGAAAG AGGAGGAGCC 
AGCGGTTCGC GGGCCGAGGA GCGGCAGTAC ATCAGCAAGT CCGCTCGGTC CTCCAGAGTC 
AACTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_608646.3
CDS	157..5085
Translation	MQRSTDRLRRLKKLKSVTGAQDGVGGGDAAATADNRTSASSDTNGAAASSGAGGNQPGTLNPEVFKHLDKSGRSEFLKYLKHQLQDAADTPVYDKNGVFKQGISNAIYQLLWQTLRFTHKKDIVLHLLLEVVALHADFPSLIVDVVNILDSETSLITDGLQEERHAFVQLVKDLDRVIPESLLKERLEIDTLQEAGIVKNKSFYSKFIKVKTKLYYKQRRFNLFREESEGFAKLITELNQEFDENTTPESIMDIIKSLIGCFNLDPNRVLDIIIESFETRPDRWNLFIPLLRSYMPTGAIICEVLGYKFCHFKDSRTPRSLYHVCALLLKHGVIALNDVYVWLTPNDGSIKADWEEDLADAREMVRKLNLIQTNKKEDEKDPPPPPSVKKFNEEKYNANQKFGLCEALLKVGDWENAYKIIQKLPEQAVVLQEPIARAIADLIHLSVENIYYKKCFKAPAGRRPSRNRLYEDSKLVAKMQAKEFGDLRKYTWPMANVLGPAMHYDTVLMYKLIRIMRKLVVDMGVDSLNGPPPNSEAEQHYYDIMSSLDACILPSLLYLDNNCSMSEEIWAVLKYFPYHFRYSLYARWKNDSYQLHPNLIRRCGLAQRDIKALMKRVSKENVKQLGRLVGKYSHCAPGLLFDYILLQIQIYDNLIGPVCDLLKYLTALSFDCLGYCIIESLTLTGRLRFKDDGTSLSLWLQSLASFCGTIYKKYSIELSGLLQYVANQLKSQKSLDLLILREIVHKMAGVESCEEMTNDQLQAMCGGEQLRGEAGYFSQVRNTKKSSNRLKEALANNDLAVAICLLMAQQKHCVIYRETAAHSHLKLVGNLYDQCQDTLVQFGTFLGSTYSVDEYVERLPSIITMLREYHINTDVAFFLARPMFTHQINQKYDQLRKDDPNAKKLTTTQKLQKYLEATQLIMNPIVESVRPLHSSKVWEDISPQFLVTFWSLSMYDLHVPNESYQREVAKLKQLAQQAAEGKDSNQSKNKKEQERYIALMEKLNDERKKQHEHVDKISQRLQEQKDSWFLLRSGKSAKNDTITQFLQLCLFPRCTFTALDALYCAKFVHTIHNLKTSNFSTLLCYDRIFCDITYSVTSCTEGEATRYGRFLCAMLETVMRWHADQAVFNKECANYPGFVTKFRVSNQFSEANDHVGYENYRHVCHKWHYKITKAIVFCLDSKDFMQIRNALIILMRILPHYPVLSKLAQIIERKVDKVREEEKTKRPDLYAIASSYIGQLKLKTPHMLKESVFHQIAERPNKESPTSVGAPAAATRSDKLSPTSPSGNTQGTRAPGGAAPFYNSEQKSVIKEPDAKAASTTRESKSQRGEGNNVTLVSTATSTASNNERESKQRDLPAPRESQSRSKDDQQEQANNGSNGSRQSESRNRDVERDRHDQQDQRSISSHRSSRDIVRVKERTEAELHQRSRERSQRLEELEAQQRKREKTSRRGGEERIRHGDGVETVDLVGSTDNRHYEEFEGRMRDLSSVSNESNGSLHHRQRSHETIEFEKVDSKRRKLESSTSSSKKVEELVDSVKKARALKTKERNKDKLSDEERDARKDRKLGRKRDRVDESNSNEHKRRREGQNGEEELRDRERHREKSPRERSHEKFDRERGGASGSRAEERQYISKSARSSRVNY

Target ORF information:

RefSeq Version	NM_134802.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tho2, transcript variant B (tho2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134802.4

ORF Insert Sequence:

ATGCAGAGAT CCACAGACCG GTTGCGCCGG CTGAAAAAGC TGAAGAGCGT TACGGGAGCG 
CAGGATGGCG TTGGTGGCGG CGATGCGGCG GCGACGGCGG ATAATCGGAC CTCCGCATCG 
TCGGACACGA ACGGAGCAGC CGCCAGTAGC GGAGCCGGCG GCAATCAGCC AGGCACACTC 
AATCCGGAGG TCTTCAAGCA CTTGGACAAA TCCGGACGCA GTGAATTCCT GAAGTACCTC 
AAGCATCAGT TGCAGGATGC GGCGGATACG CCCGTTTACG ACAAGAACGG CGTCTTTAAG 
CAGGGCATCT CCAATGCCAT ATACCAGCTT CTTTGGCAGA CTCTGCGCTT CACTCACAAA 
AAAGACATAG TACTCCACCT GCTCCTGGAA GTCGTGGCCC TCCATGCGGA CTTTCCCAGC 
CTTATAGTGG ACGTGGTTAA CATTCTGGAC AGCGAAACCT CCCTTATAAC CGATGGACTT 
CAGGAAGAAC GACATGCCTT TGTGCAACTT GTTAAAGACT TGGACAGGGT GATTCCAGAA 
AGTCTTTTGA AAGAACGTTT GGAAATCGAT ACGTTGCAGG AGGCGGGAAT CGTCAAGAAC 
AAGAGCTTTT ACTCTAAGTT CATCAAGGTC AAAACCAAGC TATACTACAA GCAGCGTCGG 
TTTAACCTTT TCCGCGAGGA GAGTGAAGGG TTTGCCAAGC TTATCACAGA GCTAAACCAG 
GAGTTCGACG AGAACACCAC TCCAGAGAGC ATAATGGATA TCATAAAATC CCTTATAGGC 
TGCTTCAATC TGGATCCTAA TCGTGTGCTG GACATTATTA TCGAGTCCTT TGAAACTCGT 
CCGGATCGAT GGAACTTGTT CATACCCCTG CTGCGCAGCT ACATGCCCAC TGGTGCCATT 
ATTTGCGAAG TCCTCGGCTA CAAGTTCTGC CACTTTAAGG ACTCCCGCAC TCCCCGCTCG 
TTATATCATG TATGTGCTCT TTTGCTTAAG CATGGCGTCA TTGCTCTGAA CGATGTATAC 
GTGTGGTTGA CTCCAAACGA TGGGAGCATT AAGGCGGACT GGGAGGAAGA CCTGGCTGAT 
GCCAGGGAAA TGGTCCGCAA GCTGAACCTT ATACAAACCA ATAAGAAGGA AGACGAAAAG 
GATCCACCAC CGCCGCCTTC AGTTAAGAAG TTCAATGAGG AAAAATACAA TGCAAACCAA 
AAATTTGGCC TCTGCGAAGC TTTGCTTAAA GTTGGCGACT GGGAGAATGC GTACAAGATC 
ATCCAGAAGC TTCCCGAGCA GGCGGTTGTA TTGCAGGAAC CCATTGCCCG TGCCATTGCC 
GATCTGATTC ACCTGTCCGT GGAGAATATT TATTACAAAA AGTGTTTTAA GGCTCCCGCA 
GGACGTAGGC CGAGCCGCAA TCGCCTGTAT GAGGATTCCA AGTTGGTGGC CAAGATGCAG 
GCAAAGGAAT TCGGCGACTT AAGGAAGTAC ACCTGGCCGA TGGCTAACGT GTTGGGACCT 
GCCATGCACT ACGACACTGT GCTGATGTAC AAACTAATCC GTATTATGCG GAAACTAGTT 
GTCGATATGG GCGTGGACAG CCTAAATGGA CCTCCGCCGA ATTCGGAAGC AGAGCAGCAC 
TACTATGACA TTATGAGCTC GCTGGACGCC TGCATTCTAC CCTCCCTTCT ATACTTGGAC 
AACAACTGTT CCATGTCCGA AGAGATCTGG GCTGTGCTTA AATATTTCCC GTATCATTTT 
CGCTACAGTT TGTATGCGCG TTGGAAGAAC GACAGCTATC AGCTGCATCC CAATTTGATC 
AGGAGATGTG GATTGGCACA ACGGGATATT AAGGCCCTAA TGAAGCGCGT GAGCAAGGAG 
AACGTCAAGC AGCTGGGACG ACTGGTAGGA AAGTATAGCC ATTGTGCGCC AGGACTGCTT 
TTCGACTATA TTCTCTTGCA AATTCAAATC TATGATAATC TAATTGGTCC GGTCTGCGAC 
CTGCTCAAGT ATCTTACTGC CTTATCCTTT GACTGTTTGG GATACTGCAT CATCGAGTCG 
TTGACGTTAA CAGGTCGTCT CAGGTTCAAG GATGACGGCA CTTCGTTATC CTTGTGGCTG 
CAAAGTCTTG CCTCCTTCTG CGGCACCATA TACAAGAAGT ACAGCATTGA GCTAAGTGGT 
CTGCTTCAGT ATGTGGCCAA TCAGTTAAAG TCGCAGAAGA GCCTGGACTT ACTGATTCTG 
AGGGAGATCG TGCATAAGAT GGCAGGCGTG GAGTCCTGCG AGGAGATGAC TAATGATCAG 
TTGCAAGCCA TGTGTGGTGG TGAGCAGCTC AGGGGAGAGG CTGGCTACTT TAGTCAAGTG 
AGGAATACGA AAAAGTCCTC TAATCGCCTA AAGGAGGCAT TGGCCAATAA CGATCTTGCC 
GTGGCTATTT GCCTTTTGAT GGCTCAACAG AAACACTGCG TCATTTATCG TGAGACGGCG 
GCACACAGCC ATTTGAAGTT AGTGGGAAAT CTGTATGATC AGTGCCAAGA CACACTCGTC 
CAGTTTGGTA CGTTCCTAGG CTCCACCTAT TCTGTGGATG AGTATGTGGA GCGGTTGCCC 
TCGATTATAA CCATGCTTCG AGAGTACCAC ATAAACACGG ATGTGGCCTT CTTCTTGGCT 
AGGCCCATGT TTACTCATCA GATCAATCAA AAATATGATC AATTGCGCAA GGATGACCCT 
AACGCCAAGA AGCTCACCAC GACGCAAAAG CTCCAGAAGT ATTTGGAAGC TACGCAGCTG 
ATCATGAACC CTATTGTAGA GTCTGTTCGT CCACTGCACA GCTCCAAGGT GTGGGAGGAC 
ATTAGTCCGC AGTTCCTGGT AACTTTTTGG AGTCTGAGCA TGTACGATCT GCACGTGCCG 
AACGAAAGCT ATCAGAGGGA GGTTGCCAAG CTAAAGCAGC TGGCCCAGCA AGCGGCAGAA 
GGCAAAGACT CTAACCAATC TAAGAACAAG AAGGAGCAGG AGCGCTACAT TGCGCTAATG 
GAGAAGCTGA ACGACGAGCG AAAGAAGCAG CATGAGCACG TTGACAAGAT CTCGCAGCGT 
TTGCAGGAGC AAAAAGATAG TTGGTTCCTT CTACGATCTG GCAAGTCCGC AAAGAACGAC 
ACCATCACGC AATTCCTGCA GCTCTGTCTC TTCCCGCGTT GCACCTTCAC AGCTCTGGAT 
GCGTTGTATT GCGCGAAGTT CGTGCACACC ATCCACAATC TCAAGACATC GAACTTCTCA 
ACCTTGCTGT GCTATGATAG GATCTTTTGT GACATCACTT ATTCGGTAAC ATCGTGCACT 
GAGGGCGAGG CTACGCGGTA CGGACGCTTT TTGTGCGCCA TGCTCGAGAC CGTGATGCGT 
TGGCATGCGG ATCAGGCGGT GTTTAATAAG GAGTGCGCCA ATTATCCTGG TTTTGTGACA 
AAGTTTCGTG TTAGCAATCA GTTCTCAGAG GCCAACGATC ATGTTGGCTA CGAAAACTAT 
CGGCATGTGT GTCACAAGTG GCACTACAAG ATTACCAAGG CGATTGTATT CTGTCTGGAC 
TCGAAGGACT TTATGCAGAT CAGAAACGCA CTGATCATCC TAATGCGAAT ACTGCCTCAC 
TATCCAGTTC TTTCCAAACT GGCGCAAATC ATCGAGCGTA AGGTGGACAA GGTGCGCGAG 
GAGGAGAAGA CAAAGCGACC GGATCTGTAT GCCATTGCCT CCAGCTACAT TGGACAACTA 
AAGCTGAAGA CTCCCCACAT GCTAAAGGAG AGTGTCTTTC ATCAAATCGC CGAGCGGCCG 
AATAAGGAGT CGCCGACCAG TGTTGGCGCT CCCGCCGCAG CCACCAGATC CGATAAGTTG 
TCACCCACCT CTCCATCTGG GAATACACAA GGAACAAGGG CTCCAGGCGG AGCTGCTCCT 
TTCTACAACA GCGAACAAAA GTCGGTGATC AAAGAACCAG ATGCAAAAGC AGCCTCCACA 
ACGCGTGAAT CGAAGAGCCA AAGAGGCGAG GGCAATAATG TCACTTTGGT GTCCACAGCC 
ACTTCAACGG CATCCAATAA CGAACGCGAG AGCAAACAAC GTGACCTGCC AGCGCCACGT 
GAGTCCCAAT CCCGCAGCAA GGATGACCAG CAGGAGCAGG CAAACAACGG CAGTAATGGC 
AGCCGACAGA GCGAGAGTCG TAATAGAGAT GTGGAACGAG ACCGACATGA TCAGCAAGAC 
CAACGAAGCA TTAGTAGCCA TCGCAGTTCC AGAGACATTG TGCGGGTCAA GGAGCGCACA 
GAAGCCGAGC TGCATCAGAG GTCGCGGGAA CGTTCTCAAC GTCTGGAGGA GTTGGAAGCG 
CAGCAGCGGA AGCGGGAAAA AACATCGCGA CGGGGCGGCG AAGAGCGCAT CCGACATGGT 
GATGGCGTCG AGACAGTGGA CCTGGTGGGC AGCACCGACA ATCGCCACTA CGAGGAGTTC 
GAGGGGCGTA TGAGGGACCT GAGCTCAGTG TCCAACGAGA GCAACGGTTC GTTGCACCAT 
CGACAACGTA GCCATGAGAC CATTGAATTT GAAAAGGTAG ATTCAAAGCG TCGCAAATTG 
GAGTCATCAA CCAGCAGTTC AAAGAAGGTG GAAGAACTGG TGGACAGCGT AAAGAAGGCG 
CGCGCCCTTA AGACCAAAGA GCGCAACAAG GACAAACTGT CGGATGAGGA GCGAGACGCT 
CGCAAGGATC GCAAGCTGGG ACGCAAGCGG GATCGCGTCG ACGAGTCCAA CAGCAACGAA 
CATAAACGCC GGCGCGAAGG ACAAAACGGT GAAGAAGAGC TGCGGGATAG AGAGCGGCAT 
AGGGAGAAGT CACCGCGGGA GCGATCACAT GAAAAGTTCG ATAGAGAAAG AGGAGGAGCC 
AGCGGTTCGC GGGCCGAGGA GCGGCAGTAC ATCAGCAAGT CCGCTCGGTC CTCCAGAGTC 
AACTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tho2 ( NM_001272976.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259905.1 Drosophila melanogaster tho2, transcript variant C (tho2), mRNA.

Price & Availability↑

CloneID	OFa15990
Clone ID Related Accession (Same CDS sequence)	NM_001272976.1
Accession Version	NM_001272976.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tho2, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGAGAT CCACAGACCG GTTGCGCCGG CTGAAAAAGC TGAAGAGCGT TACGGGAGCG 
CAGGATGGCG TTGGTGGCGG CGATGCGGCG GCGACGGCGG ATAATCGGAC CTCCGCATCG 
TCGGACACGA ACGGAGCAGC CGCCAGTAGC GGAGCCGGCG GCAATCAGCC AGGCACACTC 
AATCCGGAGG TCTTCAAGCA CTTGGACAAA TCCGGACGCA GTGAATTCCT GAAGTACCTC 
AAGCATCAGT TGCAGGATGC GGCGGATACG CCCGTTTACG ACAAGAACGG CGTCTTTAAG 
CAGGGCATCT CCAATGCCAT ATACCAGCTT CTTTGGCAGA CTCTGCGCTT CACTCACAAA 
AAAGACATAG TACTCCACCT GCTCCTGGAA GTCGTGGCCC TCCATGCGGA CTTTCCCAGC 
CTTATAGTGG ACGTGGTTAA CATTCTGGAC AGCGAAACCT CCCTTATAAC CGATGGACTT 
CAGGAAGAAC GACATGCCTT TGTGCAACTT GTTAAAGACT TGGACAGGGT GATTCCAGAA 
AGTCTTTTGA AAGAACGTTT GGAAATCGAT ACGTTGCAGG AGGCGGGAAT CGTCAAGAAC 
AAGAGCTTTT ACTCTAAGTT CATCAAGGTC AAAACCAAGC TATACTACAA GCAGCGTCGG 
TTTAACCTTT TCCGCGAGGA GAGTGAAGGG TTTGCCAAGC TTATCACAGA GCTAAACCAG 
GAGTTCGACG AGAACACCAC TCCAGAGAGC ATAATGGATA TCATAAAATC CCTTATAGGC 
TGCTTCAATC TGGATCCTAA TCGTGTGCTG GACATTATTA TCGAGTCCTT TGAAACTCGT 
CCGGATCGAT GGAACTTGTT CATACCCCTG CTGCGCAGCT ACATGCCCAC TGGTGCCATT 
ATTTGCGAAG TCCTCGGCTA CAAGTTCTGC CACTTTAAGG ACTCCCGCAC TCCCCGCTCG 
TTATATCATG TATGTGCTCT TTTGCTTAAG CATGGCGTCA TTGCTCTGAA CGATGTATAC 
GTGTGGTTGA CTCCAAACGA TGGGAGCATT AAGGCGGACT GGGAGGAAGA CCTGGCTGAT 
GCCAGGGAAA TGGTCCGCAA GCTGAACCTT ATACAAACCA ATAAGAAGGA AGACGAAAAG 
GATCCACCAC CGCCGCCTTC AGTTAAGAAG TTCAATGAGG AAAAATACAA TGCAAACCAA 
AAATTTGGCC TCTGCGAAGC TTTGCTTAAA GTTGGCGACT GGGAGAATGC GTACAAGATC 
ATCCAGAAGC TTCCCGAGCA GGCGGTTGTA TTGCAGGAAC CCATTGCCCG TGCCATTGCC 
GATCTGATTC ACCTGTCCGT GGAGAATATT TATTACAAAA AGTGTTTTAA GGCTCCCGCA 
GGACGTAGGC CGAGCCGCAA TCGCCTGTAT GAGGATTCCA AGTTGGTGGC CAAGATGCAG 
GCAAAGGAAT TCGGCGACTT AAGGAAGTAC ACCTGGCCGA TGGCTAACGT GTTGGGACCT 
GCCATGCACT ACGACACTGT GCTGATGTAC AAACTAATCC GTATTATGCG GAAACTAGTT 
GTCGATATGG GCGTGGACAG CCTAAATGGA CCTCCGCCGA ATTCGGAAGC AGAGCAGCAC 
TACTATGACA TTATGAGCTC GCTGGACGCC TGCATTCTAC CCTCCCTTCT ATACTTGGAC 
AACAACTGTT CCATGTCCGA AGAGATCTGG GCTGTGCTTA AATATTTCCC GTATCATTTT 
CGCTACAGTT TGTATGCGCG TTGGAAGAAC GACAGCTATC AGCTGCATCC CAATTTGATC 
AGGAGATGTG GATTGGCACA ACGGGATATT AAGGCCCTAA TGAAGCGCGT GAGCAAGGAG 
AACGTCAAGC AGCTGGGACG ACTGGTAGGA AAGTATAGCC ATTGTGCGCC AGGACTGCTT 
TTCGACTATA TTCTCTTGCA AATTCAAATC TATGATAATC TAATTGGTCC GGTCTGCGAC 
CTGCTCAAGT ATCTTACTGC CTTATCCTTT GACTGTTTGG GATACTGCAT CATCGAGTCG 
TTGACGTTAA CAGGTCGTCT CAGGTTCAAG GATGACGGCA CTTCGTTATC CTTGTGGCTG 
CAAAGTCTTG CCTCCTTCTG CGGCACCATA TACAAGAAGT ACAGCATTGA GCTAAGTGGT 
CTGCTTCAGT ATGTGGCCAA TCAGTTAAAG TCGCAGAAGA GCCTGGACTT ACTGATTCTG 
AGGGAGATCG TGCATAAGAT GGCAGGCGTG GAGTCCTGCG AGGAGATGAC TAATGATCAG 
TTGCAAGCCA TGTGTGGTGG TGAGCAGCTC AGGGGAGAGG CTGGCTACTT TAGTCAAGTG 
AGGAATACGA AAAAGTCCTC TAATCGCCTA AAGGAGGCAT TGGCCAATAA CGATCTTGCC 
GTGGCTATTT GCCTTTTGAT GGCTCAACAG AAACACTGCG TCATTTATCG TGAGACGGCG 
GCACACAGCC ATTTGAAGTT AGTGGGAAAT CTGTATGATC AGTGCCAAGA CACACTCGTC 
CAGTTTGGTA CGTTCCTAGG CTCCACCTAT TCTGTGGATG AGTATGTGGA GCGGTTGCCC 
TCGATTATAA CCATGCTTCG AGAGTACCAC ATAAACACGG ATGTGGCCTT CTTCTTGGCT 
AGGCCCATGT TTACTCATCA GATCAATCAA AAATATGATC AATTGCGCAA GGATGACCCT 
AACGCCAAGA AGCTCACCAC GACGCAAAAG CTCCAGAAGT ATTTGGAAGC TACGCAGCTG 
ATCATGAACC CTATTGTAGA GTCTGTTCGT CCACTGCACA GCTCCAAGGT GTGGGAGGAC 
ATTAGTCCGC AGTTCCTGGT AACTTTTTGG AGTCTGAGCA TGTACGATCT GCACGTGCCG 
AACGAAAGCT ATCAGAGGGA GGTTGCCAAG CTAAAGCAGC TGGCCCAGCA AGCGGCAGAA 
GGCAAAGACT CTAACCAATC TAAGAACAAG AAGGAGCAGG AGCGCTACAT TGCGCTAATG 
GAGAAGCTGA ACGACGAGCG AAAGAAGCAG CATGAGCACG TTGACAAGAT CTCGCAGCGT 
TTGCAGGAGC AAAAAGATAG TTGGTTCCTT CTACGATCTG GCAAGTCCGC AAAGAACGAC 
ACCATCACGC AATTCCTGCA GCTCTGTCTC TTCCCGCGTT GCACCTTCAC AGCTCTGGAT 
GCGTTGTATT GCGCGAAGTT CGTGCACACC ATCCACAATC TCAAGACATC GAACTTCTCA 
ACCTTGCTGT GCTATGATAG GATCTTTTGT GACATCACTT ATTCGGTAAC ATCGTGCACT 
GAGGGCGAGG CTACGCGGTA CGGACGCTTT TTGTGCGCCA TGCTCGAGAC CGTGATGCGT 
TGGCATGCGG ATCAGGCGGT GTTTAATAAG GAGTGCGCCA ATTATCCTGG TTTTGTGACA 
AAGTTTCGTG TTAGCAATCA GTTCTCAGAG GCCAACGATC ATGTTGGCTA CGAAAACTAT 
CGGCATGTGT GTCACAAGTG GCACTACAAG ATTACCAAGG CGATTGTATT CTGTCTGGAC 
TCGAAGGACT TTATGCAGAT CAGAAACGCA CTGATCATCC TAATGCGAAT ACTGCCTCAC 
TATCCAGTTC TTTCCAAACT GGCGCAAATC ATCGAGCGTA AGGTGGACAA GGTGCGCGAG 
GAGGAGAAGA CAAAGCGACC GGATCTGTAT GCCATTGCCT CCAGCTACAT TGGACAACTA 
AAGCTGAAGA CTCCCCACAT GCTAAAGGAG AGTGTCTTTC ATCAAATCGC CGAGCGGCCG 
AATAAGGAGT CGCCGACCAG TGTTGGCGCT CCCGCCGCAG CCACCAGATC CGATAAGTTG 
TCACCCACCT CTCCATCTGG GAATACACAA GGAACAAGGG CTCCAGGCGG AGCTGCTCCT 
TTCTACAACA GCGAACAAAA GTCGGTGATC AAAGAACCAG ATGCAAAAGC AGCCTCCACA 
ACGCGTGAAT CGAAGAGCCA AAGAGGCGAG GGCAATAATG TCACTTTGGT GTCCACAGCC 
ACTTCAACGG CATCCAATAA CGAACGCGAG AGCAAACAAC GTGACCTGCC AGCGCCACGT 
GAGTCCCAAT CCCGCAGCAA GGATGACCAG CAGGAGCAGG CAAACAACGG CAGTAATGGC 
AGCCGACAGA GCGAGAGTCG TAATAGAGAT GTGGAACGAG ACCGACATGA TCAGCAAGAC 
CAACGAAGCA TTAGTAGCCA TCGCAGTTCC AGAGACATTG TGCGGGTCAA GGAGCGCACA 
GAAGCCGAGC TGCATCAGAG GTCGCGGGAA CGTTCTCAAC GTCTGGAGGA GTTGGAAGCG 
CAGCAGCGGA AGCGGGAAAA AACATCGCGA CGGGGCGGCG AAGAGCGCAT CCGACATGGT 
GATGGCGTCG AGACAGTGGA CCTGGTGGGC AGCACCGACA ATCGCCACTA CGAGGAGTTC 
GAGGGGCGTA TGAGGGACCT GAGCTCAGTG TCCAACGAGA GCAACGGTTC GTTGCACCAT 
CGACAACGTA GCCATGAGAC CATTGAATTT GAAAAGGTAG ATTCAAAGCG TCGCAAATTG 
GAGTCATCAA CCAGCAGTTC AAAGGTGGAA GAACTGGTGG ACAGCGTAAA GAAGGCGCGC 
GCCCTTAAGA CCAAAGAGCG CAACAAGGAC AAACTGTCGG ATGAGGAGCG AGACGCTCGC 
AAGGATCGCA AGCTGGGACG CAAGCGGGAT CGCGTCGACG AGTCCAACAG CAACGAACAT 
AAACGCCGGC GCGAAGGACA AAACGGTGAA GAAGAGCTGC GGGATAGAGA GCGGCATAGG 
GAGAAGTCAC CGCGGGAGCG ATCACATGAA AAGTTCGATA GAGAAAGAGG AGGAGCCAGC 
GGTTCGCGGG CCGAGGAGCG GCAGTACATC AGCAAGTCCG CTCGGTCCTC CAGAGTCAAC 
TATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259905.1
CDS	157..5082
Translation	MQRSTDRLRRLKKLKSVTGAQDGVGGGDAAATADNRTSASSDTNGAAASSGAGGNQPGTLNPEVFKHLDKSGRSEFLKYLKHQLQDAADTPVYDKNGVFKQGISNAIYQLLWQTLRFTHKKDIVLHLLLEVVALHADFPSLIVDVVNILDSETSLITDGLQEERHAFVQLVKDLDRVIPESLLKERLEIDTLQEAGIVKNKSFYSKFIKVKTKLYYKQRRFNLFREESEGFAKLITELNQEFDENTTPESIMDIIKSLIGCFNLDPNRVLDIIIESFETRPDRWNLFIPLLRSYMPTGAIICEVLGYKFCHFKDSRTPRSLYHVCALLLKHGVIALNDVYVWLTPNDGSIKADWEEDLADAREMVRKLNLIQTNKKEDEKDPPPPPSVKKFNEEKYNANQKFGLCEALLKVGDWENAYKIIQKLPEQAVVLQEPIARAIADLIHLSVENIYYKKCFKAPAGRRPSRNRLYEDSKLVAKMQAKEFGDLRKYTWPMANVLGPAMHYDTVLMYKLIRIMRKLVVDMGVDSLNGPPPNSEAEQHYYDIMSSLDACILPSLLYLDNNCSMSEEIWAVLKYFPYHFRYSLYARWKNDSYQLHPNLIRRCGLAQRDIKALMKRVSKENVKQLGRLVGKYSHCAPGLLFDYILLQIQIYDNLIGPVCDLLKYLTALSFDCLGYCIIESLTLTGRLRFKDDGTSLSLWLQSLASFCGTIYKKYSIELSGLLQYVANQLKSQKSLDLLILREIVHKMAGVESCEEMTNDQLQAMCGGEQLRGEAGYFSQVRNTKKSSNRLKEALANNDLAVAICLLMAQQKHCVIYRETAAHSHLKLVGNLYDQCQDTLVQFGTFLGSTYSVDEYVERLPSIITMLREYHINTDVAFFLARPMFTHQINQKYDQLRKDDPNAKKLTTTQKLQKYLEATQLIMNPIVESVRPLHSSKVWEDISPQFLVTFWSLSMYDLHVPNESYQREVAKLKQLAQQAAEGKDSNQSKNKKEQERYIALMEKLNDERKKQHEHVDKISQRLQEQKDSWFLLRSGKSAKNDTITQFLQLCLFPRCTFTALDALYCAKFVHTIHNLKTSNFSTLLCYDRIFCDITYSVTSCTEGEATRYGRFLCAMLETVMRWHADQAVFNKECANYPGFVTKFRVSNQFSEANDHVGYENYRHVCHKWHYKITKAIVFCLDSKDFMQIRNALIILMRILPHYPVLSKLAQIIERKVDKVREEEKTKRPDLYAIASSYIGQLKLKTPHMLKESVFHQIAERPNKESPTSVGAPAAATRSDKLSPTSPSGNTQGTRAPGGAAPFYNSEQKSVIKEPDAKAASTTRESKSQRGEGNNVTLVSTATSTASNNERESKQRDLPAPRESQSRSKDDQQEQANNGSNGSRQSESRNRDVERDRHDQQDQRSISSHRSSRDIVRVKERTEAELHQRSRERSQRLEELEAQQRKREKTSRRGGEERIRHGDGVETVDLVGSTDNRHYEEFEGRMRDLSSVSNESNGSLHHRQRSHETIEFEKVDSKRRKLESSTSSSKVEELVDSVKKARALKTKERNKDKLSDEERDARKDRKLGRKRDRVDESNSNEHKRRREGQNGEEELRDRERHREKSPRERSHEKFDRERGGASGSRAEERQYISKSARSSRVNY

Target ORF information:

RefSeq Version	NM_001272976.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tho2, transcript variant C (tho2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272976.1

ORF Insert Sequence:

ATGCAGAGAT CCACAGACCG GTTGCGCCGG CTGAAAAAGC TGAAGAGCGT TACGGGAGCG 
CAGGATGGCG TTGGTGGCGG CGATGCGGCG GCGACGGCGG ATAATCGGAC CTCCGCATCG 
TCGGACACGA ACGGAGCAGC CGCCAGTAGC GGAGCCGGCG GCAATCAGCC AGGCACACTC 
AATCCGGAGG TCTTCAAGCA CTTGGACAAA TCCGGACGCA GTGAATTCCT GAAGTACCTC 
AAGCATCAGT TGCAGGATGC GGCGGATACG CCCGTTTACG ACAAGAACGG CGTCTTTAAG 
CAGGGCATCT CCAATGCCAT ATACCAGCTT CTTTGGCAGA CTCTGCGCTT CACTCACAAA 
AAAGACATAG TACTCCACCT GCTCCTGGAA GTCGTGGCCC TCCATGCGGA CTTTCCCAGC 
CTTATAGTGG ACGTGGTTAA CATTCTGGAC AGCGAAACCT CCCTTATAAC CGATGGACTT 
CAGGAAGAAC GACATGCCTT TGTGCAACTT GTTAAAGACT TGGACAGGGT GATTCCAGAA 
AGTCTTTTGA AAGAACGTTT GGAAATCGAT ACGTTGCAGG AGGCGGGAAT CGTCAAGAAC 
AAGAGCTTTT ACTCTAAGTT CATCAAGGTC AAAACCAAGC TATACTACAA GCAGCGTCGG 
TTTAACCTTT TCCGCGAGGA GAGTGAAGGG TTTGCCAAGC TTATCACAGA GCTAAACCAG 
GAGTTCGACG AGAACACCAC TCCAGAGAGC ATAATGGATA TCATAAAATC CCTTATAGGC 
TGCTTCAATC TGGATCCTAA TCGTGTGCTG GACATTATTA TCGAGTCCTT TGAAACTCGT 
CCGGATCGAT GGAACTTGTT CATACCCCTG CTGCGCAGCT ACATGCCCAC TGGTGCCATT 
ATTTGCGAAG TCCTCGGCTA CAAGTTCTGC CACTTTAAGG ACTCCCGCAC TCCCCGCTCG 
TTATATCATG TATGTGCTCT TTTGCTTAAG CATGGCGTCA TTGCTCTGAA CGATGTATAC 
GTGTGGTTGA CTCCAAACGA TGGGAGCATT AAGGCGGACT GGGAGGAAGA CCTGGCTGAT 
GCCAGGGAAA TGGTCCGCAA GCTGAACCTT ATACAAACCA ATAAGAAGGA AGACGAAAAG 
GATCCACCAC CGCCGCCTTC AGTTAAGAAG TTCAATGAGG AAAAATACAA TGCAAACCAA 
AAATTTGGCC TCTGCGAAGC TTTGCTTAAA GTTGGCGACT GGGAGAATGC GTACAAGATC 
ATCCAGAAGC TTCCCGAGCA GGCGGTTGTA TTGCAGGAAC CCATTGCCCG TGCCATTGCC 
GATCTGATTC ACCTGTCCGT GGAGAATATT TATTACAAAA AGTGTTTTAA GGCTCCCGCA 
GGACGTAGGC CGAGCCGCAA TCGCCTGTAT GAGGATTCCA AGTTGGTGGC CAAGATGCAG 
GCAAAGGAAT TCGGCGACTT AAGGAAGTAC ACCTGGCCGA TGGCTAACGT GTTGGGACCT 
GCCATGCACT ACGACACTGT GCTGATGTAC AAACTAATCC GTATTATGCG GAAACTAGTT 
GTCGATATGG GCGTGGACAG CCTAAATGGA CCTCCGCCGA ATTCGGAAGC AGAGCAGCAC 
TACTATGACA TTATGAGCTC GCTGGACGCC TGCATTCTAC CCTCCCTTCT ATACTTGGAC 
AACAACTGTT CCATGTCCGA AGAGATCTGG GCTGTGCTTA AATATTTCCC GTATCATTTT 
CGCTACAGTT TGTATGCGCG TTGGAAGAAC GACAGCTATC AGCTGCATCC CAATTTGATC 
AGGAGATGTG GATTGGCACA ACGGGATATT AAGGCCCTAA TGAAGCGCGT GAGCAAGGAG 
AACGTCAAGC AGCTGGGACG ACTGGTAGGA AAGTATAGCC ATTGTGCGCC AGGACTGCTT 
TTCGACTATA TTCTCTTGCA AATTCAAATC TATGATAATC TAATTGGTCC GGTCTGCGAC 
CTGCTCAAGT ATCTTACTGC CTTATCCTTT GACTGTTTGG GATACTGCAT CATCGAGTCG 
TTGACGTTAA CAGGTCGTCT CAGGTTCAAG GATGACGGCA CTTCGTTATC CTTGTGGCTG 
CAAAGTCTTG CCTCCTTCTG CGGCACCATA TACAAGAAGT ACAGCATTGA GCTAAGTGGT 
CTGCTTCAGT ATGTGGCCAA TCAGTTAAAG TCGCAGAAGA GCCTGGACTT ACTGATTCTG 
AGGGAGATCG TGCATAAGAT GGCAGGCGTG GAGTCCTGCG AGGAGATGAC TAATGATCAG 
TTGCAAGCCA TGTGTGGTGG TGAGCAGCTC AGGGGAGAGG CTGGCTACTT TAGTCAAGTG 
AGGAATACGA AAAAGTCCTC TAATCGCCTA AAGGAGGCAT TGGCCAATAA CGATCTTGCC 
GTGGCTATTT GCCTTTTGAT GGCTCAACAG AAACACTGCG TCATTTATCG TGAGACGGCG 
GCACACAGCC ATTTGAAGTT AGTGGGAAAT CTGTATGATC AGTGCCAAGA CACACTCGTC 
CAGTTTGGTA CGTTCCTAGG CTCCACCTAT TCTGTGGATG AGTATGTGGA GCGGTTGCCC 
TCGATTATAA CCATGCTTCG AGAGTACCAC ATAAACACGG ATGTGGCCTT CTTCTTGGCT 
AGGCCCATGT TTACTCATCA GATCAATCAA AAATATGATC AATTGCGCAA GGATGACCCT 
AACGCCAAGA AGCTCACCAC GACGCAAAAG CTCCAGAAGT ATTTGGAAGC TACGCAGCTG 
ATCATGAACC CTATTGTAGA GTCTGTTCGT CCACTGCACA GCTCCAAGGT GTGGGAGGAC 
ATTAGTCCGC AGTTCCTGGT AACTTTTTGG AGTCTGAGCA TGTACGATCT GCACGTGCCG 
AACGAAAGCT ATCAGAGGGA GGTTGCCAAG CTAAAGCAGC TGGCCCAGCA AGCGGCAGAA 
GGCAAAGACT CTAACCAATC TAAGAACAAG AAGGAGCAGG AGCGCTACAT TGCGCTAATG 
GAGAAGCTGA ACGACGAGCG AAAGAAGCAG CATGAGCACG TTGACAAGAT CTCGCAGCGT 
TTGCAGGAGC AAAAAGATAG TTGGTTCCTT CTACGATCTG GCAAGTCCGC AAAGAACGAC 
ACCATCACGC AATTCCTGCA GCTCTGTCTC TTCCCGCGTT GCACCTTCAC AGCTCTGGAT 
GCGTTGTATT GCGCGAAGTT CGTGCACACC ATCCACAATC TCAAGACATC GAACTTCTCA 
ACCTTGCTGT GCTATGATAG GATCTTTTGT GACATCACTT ATTCGGTAAC ATCGTGCACT 
GAGGGCGAGG CTACGCGGTA CGGACGCTTT TTGTGCGCCA TGCTCGAGAC CGTGATGCGT 
TGGCATGCGG ATCAGGCGGT GTTTAATAAG GAGTGCGCCA ATTATCCTGG TTTTGTGACA 
AAGTTTCGTG TTAGCAATCA GTTCTCAGAG GCCAACGATC ATGTTGGCTA CGAAAACTAT 
CGGCATGTGT GTCACAAGTG GCACTACAAG ATTACCAAGG CGATTGTATT CTGTCTGGAC 
TCGAAGGACT TTATGCAGAT CAGAAACGCA CTGATCATCC TAATGCGAAT ACTGCCTCAC 
TATCCAGTTC TTTCCAAACT GGCGCAAATC ATCGAGCGTA AGGTGGACAA GGTGCGCGAG 
GAGGAGAAGA CAAAGCGACC GGATCTGTAT GCCATTGCCT CCAGCTACAT TGGACAACTA 
AAGCTGAAGA CTCCCCACAT GCTAAAGGAG AGTGTCTTTC ATCAAATCGC CGAGCGGCCG 
AATAAGGAGT CGCCGACCAG TGTTGGCGCT CCCGCCGCAG CCACCAGATC CGATAAGTTG 
TCACCCACCT CTCCATCTGG GAATACACAA GGAACAAGGG CTCCAGGCGG AGCTGCTCCT 
TTCTACAACA GCGAACAAAA GTCGGTGATC AAAGAACCAG ATGCAAAAGC AGCCTCCACA 
ACGCGTGAAT CGAAGAGCCA AAGAGGCGAG GGCAATAATG TCACTTTGGT GTCCACAGCC 
ACTTCAACGG CATCCAATAA CGAACGCGAG AGCAAACAAC GTGACCTGCC AGCGCCACGT 
GAGTCCCAAT CCCGCAGCAA GGATGACCAG CAGGAGCAGG CAAACAACGG CAGTAATGGC 
AGCCGACAGA GCGAGAGTCG TAATAGAGAT GTGGAACGAG ACCGACATGA TCAGCAAGAC 
CAACGAAGCA TTAGTAGCCA TCGCAGTTCC AGAGACATTG TGCGGGTCAA GGAGCGCACA 
GAAGCCGAGC TGCATCAGAG GTCGCGGGAA CGTTCTCAAC GTCTGGAGGA GTTGGAAGCG 
CAGCAGCGGA AGCGGGAAAA AACATCGCGA CGGGGCGGCG AAGAGCGCAT CCGACATGGT 
GATGGCGTCG AGACAGTGGA CCTGGTGGGC AGCACCGACA ATCGCCACTA CGAGGAGTTC 
GAGGGGCGTA TGAGGGACCT GAGCTCAGTG TCCAACGAGA GCAACGGTTC GTTGCACCAT 
CGACAACGTA GCCATGAGAC CATTGAATTT GAAAAGGTAG ATTCAAAGCG TCGCAAATTG 
GAGTCATCAA CCAGCAGTTC AAAGGTGGAA GAACTGGTGG ACAGCGTAAA GAAGGCGCGC 
GCCCTTAAGA CCAAAGAGCG CAACAAGGAC AAACTGTCGG ATGAGGAGCG AGACGCTCGC 
AAGGATCGCA AGCTGGGACG CAAGCGGGAT CGCGTCGACG AGTCCAACAG CAACGAACAT 
AAACGCCGGC GCGAAGGACA AAACGGTGAA GAAGAGCTGC GGGATAGAGA GCGGCATAGG 
GAGAAGTCAC CGCGGGAGCG ATCACATGAA AAGTTCGATA GAGAAAGAGG AGGAGCCAGC 
GGTTCGCGGG CCGAGGAGCG GCAGTACATC AGCAAGTCCG CTCGGTCCTC CAGAGTCAAC 
TATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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tho2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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