hang cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	hang
Entrez Gene ID	32613
Full Name	hangover
Synonyms	BcDNA:GM07804,BcDNA:LD22118,CG32575,CG4411,CG4416,Dmel\CG32575,HANG,LD22118
General protein information	Preferred Names hangover Names CG32575 gene product from transcript CG32575-RC CG32575-PA CG32575-PC CG32575-PD hang-PA hang-PC hang-PD
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 14C4-14C4

mRNA and Protein(s)

mRNA	Protein	Name
NM_167521.3	NP_727980.2	hangover, isoform A
NM_001144732.2	NP_001138204.1	hangover, isoform C
NM_167520.3	NP_727979.3	hangover, isoform D

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Hangover acts as a nuclear RNA binding protein and we identified the phosphodiesterase 4d ortholog dunce as a target RNA.
Title: Hangover Links Nuclear RNA Signaling to cAMP Regulation via the Phosphodiesterase 4d Ortholog dunce.

The increase in bouton number in hang mutants is caused by a reduction in FASII expression, thus, linking the regulation of nuclear gene expression with the addition of boutons at the NMJ regulated by cell adhesion molecules.
Title: The hangover gene negatively regulates bouton addition at the Drosophila neuromuscular junction.

ethanol tolerance in Drosophila relies on two distinct molecular pathways: a cellular stress pathway defined by hangover, and a parallel pathway requiring octopamine
Title: The hangover gene defines a stress pathway required for ethanol tolerance development.

The following hang gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hang cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01799	NM_001144732.2 Latest version!	Drosophila melanogaster hangover (hang), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OFa01801	NM_167521.3 Latest version!	Drosophila melanogaster hangover (hang), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OFa21500	NM_167520.3 Latest version!	Drosophila melanogaster hangover (hang), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hang ( NM_001144732.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001138204.1 Drosophila melanogaster hangover (hang), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa01799
Clone ID Related Accession (Same CDS sequence)	NM_001144732.2
Accession Version	NM_001144732.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	hangover, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001144732.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGCGACG CTGCGGCGGC AACAGCGACA ACAACAACAA CAGCAGCAGT AGCAGCAGCC 
GTCGCAACAA CAACAGCATC GGTAGCATTG GAGGCCACAG CGACACAGCC TGGAACGACA 
ACGACGACGG TAGCCACTGC GTCAGCGGGA ACCACATCCC CTGAAGCGGC CATTCCAACA 
GCAGCAACAG CAACATCGGC GAGAAACAGC AACAGCGAAC GGTCGGCGCG ACAAAACTGC 
TGTCGCCTGT GCATCGCCCC ACAAACCGAA TGCATCTCGA TCATCAATAG CTATGCGGCC 
GACAAGGAGC CGCTGTCCAC CAAGATCCAC AACTGCGTCG GCATCAAGGT TACACCGCAG 
GATCGGCTGT CGCAGCAAAT TTGCCACGCC TGCATCAGTT ACCTGAACTC ATGGCAGAGC 
TTCAAGAACC GGTGCTTCAG CTCGCAAGCG AAGCAGCGCC AGTGGCTGGA TACCAACAAG 
AGCAAGCTCC TCAACTACCT GGACCTGAAC AGTGCCGAGA ATGGGGGAGG AGGCTTCTTC 
GATCAGCATC TGCATCAACA ACAGCAACAC CACCAGCACT TGGAGAATGA GCTCGAAGCG 
GAGAAGGAGA AGGCGACGCC AACGGCCGCA TCGACAGCCG CCAACATATT GGACGGCATA 
CACTCGCTGA AGAAACGCAA ATCCCTAACA GTCTATGTGA GTCCTAAACC GCAGCAGATG 
CAACAGCATC AATTGCAGCA GTTGCAGCTG CAACCACCGG CCCGAATGAC CGCGACGATC 
AGCAGTGCTC AGCAATCGCT GCAGACCCTT CTCTTGCAGG TACCAGCATC GGCGGCGGCT 
AGCTGCTCAT CCACATCCAC GTCCACCACA TCCGCATCCC AGCAGCGGGA ACAGCAGCAG 
CATCAGCAGC GCCAGCAATC TCACCAGTAT CGCAGATACC ATCATCATCA TCAGCAGCAG 
CAGCAACAGC AGCAGCAGCA GCATAATCAG CGTGGCAAGC AAATGTTGCC ACATCATCAG 
CAGCATCAGC AACAGCACCA CAGACGCCAG CGACAATTTC GCCCACAGCG TCCACTTCCA 
GTTCCGGTGG TCAATCCGGC TCCAACATTG CGCAAATTTA AGCCAAAGCT ATATTTGCCA 
TTGGACATAA CAGTTCCTCC GTTGCCACCG CTGCCAACTA TACCATCGAT TCCAACATTG 
CCCCCAGTGG GCCCGGGATC CATTGCTCCG CCTCCTGCTG CTGAACTGCT GCCGGGAATT 
TCGGGTGTGG CTGGAATGGT GGAGAGCCTG TCGCTGTCGC TGCCGCTTGA TTTCAGCCAG 
AGCTGCAGCA GCAGCGGTCA CGTCCAACAT CAGCAGGCTG GTGGACTGGC CACGTCGACG 
ACCACGTCCA ATCCGCCGGG CAGCGTCGTT TCCGCCACGC CTTCTGCATC TGTATCTGCT 
TCTGCTTCTG CTTCCGCATC CGTATCAACG GCTCCGCTGC TTATGCCGCC ACTGCCTGCG 
ATGCCCATCA AAGATGAGCC GATTGATACG GATGACGACT ATCAGATGAA GTCCATAGAC 
GAGTCCGATG ACATGGTCGA TCCCACAATG TTCCTAGAGC GCTCCGAGCA CGAGGGTGAT 
GTCCCGCTGA CGGCCTCGGA TTACGACTAC ACTGCGCAGC ATGGCGTGAA TGCTTCGTCC 
GTGGCTGCCT CGCTGCCGCC GAATGCGGTG GCCAATGTGG CAGCCGCCGG GGACTCCAAG 
GTGGCCAGCT GCAGGGCCTG CAGCCTGCAG TTCTCTACCC GGGCCAACGC CCGTCGCCAC 
GAAAGGAATC TACACCCAAA CCTGTTCCAG TTGTCCACAG ACTCCCCCCA CAACACACCC 
ATCACAAAGC CAACGCCCGC TTTGGCTGCC GCCTTGGAAA TGCAACGGGC AGCGGCTGCG 
GCGGCCACCG CTGAGGCAAA TCGGGCAGCG GGCGCCGCTG GCGGGAATAT CTCCACGCAA 
AAGTACCGCC AGGTGGTGAT GAACGCGTTC ATTAAGTGCG AGGGCGGCGG CTACGACTAC 
GATAATCCCG AACAGTACCG ACCATTGCTC ACCCGCGACA AAGTGGAGTT CATTGAGCAG 
AACGATGAGT TCCTCGAGCA ATACCAGACG ATGACCTGCC GATGTTGCAA CAAATACTTC 
AGCACGTACA AGAACTTCAT GGCGCACGTT CGCAAGAAGT ATCCGCAGCT GCCGCGCAAC 
CTATGCTTCA ATTGCCTCAA GATGAACGAC TCCAAGGCGC TGTTCATCTC GCATCTGAAG 
AAGCGGAATT GCATAAATCT CTTTAGAGTC TTGAATGCGT TGCGTGGGAA AACAACGACA 
GTGGTTGTGC CCATTGCAGA TGATGTGGCA GACGATGGAG CAACAGGATC TATTCCAGTT 
GCCGATGCCG GAGCAGGAGT GGTGGCCATG AATAGTCCCA CAGTGACAGC CAGCGGAGAA 
GTTGTTACAC CCGGCGGCGG CTCCGAGCGC CCAGAGAAAC TGCGCGCCAA GGAACTTCTG 
GTCAACAAAC TGTACGAGTG CAAGCTCTGT CCCAAGGGAT TCCGCACTAA GCACGAGTTC 
CGAACGCATG TCTACGACAA GCACGCAGAT GTCCAACGCA AGGATAACAA CTCGATACAG 
TGCAGCTTTT GCGGACTGGA CTTTGCCGAT CCCGTTGACA GACGGCGTCA CTACAACAAC 
ATGGACTGCA TTGTCCGGCT GCGCTGCATG ACGTGCGATG CCAAGCTGGA AACGCACCAG 
CGATTCCTCG ATCATGTCTA CCAGGATCAC TTGGGCGGTG TGGGTGGTGG CGCGGTCAGC 
GACAACGCCT CCACCACTGG CAGCGGCATG GCCAGGAGCA ACAGCATGGA ACACTCGCCG 
GGCAAAAGGA GCCTGCTCGG CGCCTTAGGC GTTGGTTCCT CGGCGGAGGA GTCGCGAAGC 
AGCAGTGCGG CTCCGCCGCT GACCTCTACA CCAAAACTGG CGGGCGGTAA TCAGGTGGGT 
GGCGGTGGAT CGACCAGCGC TTCTGCCGCC GCAGCCGCTC AGAGCTCGGC GAATCGGGAT 
GCATCCGCAC CCAAATCCCA GTACTTCTCC CGTATGCCGC AGGTTTGTCC CATTTGCGGC 
CAGCAGTACA ACAACTACAA CAATGTGCTG CGCCACATGG AATCGAAGCA TCCGAACAAA 
CTGCCAGAGA CATACAAGTG CGTGCGCTGC GGACTGGGCT ATCCCCGGAT CTCCTACCTA 
CGGGAGCACA TGATCAATGT GCACGGAGTG GACAAGAACC GTCACTCTGG CGGCTTCGAA 
TACATCGTGA ATGCGGATGC CGTGAAGTTG GCGGACGGAA GCACGCCGAA CGTCTACACG 
GGTCGCTACG ATTACGTGAT GAAGGACCTG ATGTCGATAA CAAATGGTGG GACACTCGAT 
GATGAAGAGG AGGAGCCTGG CAGCGTGGCC AAGAAGATGC GCCTGGATGA CAGTAGCAAC 
AACAGCAGCC TGGTGGGCGT GGCTAGCCAG CAGAAGGAGT GCCCCATCTG CAATGCGGTG 
TTCAGCAACA ACATTGGCCT GTCCAATCAT ATGCGTTCCC ACTATACGGC CTCGAATGCA 
GTGAATGCAG CCTTGGCGGC TGCCAATCGA ATGACACCCA AATCGCTAAC GATAACCGCA 
ACGCCAGCAA CGGATTCGGA GTTGGGAGTT GGAGGAACAA TGTCCGAGTC GGCTCCAGCA 
ACGCCTGCCA ATGTGCCACC GGCAATGGCT AACCAAACGC CCCAGGAGCA GGCAGTTTTT 
CGCCGGAGCC TTGACCAGGC AGCCGATCGC CGCTTCCGGC GAATGCGATG CCGCATCTGC 
CAGCGTCGCT TCAGTTCGAA GAAGTCCTAT CGCTACCACA TGCTCACCGA TCATCAGGTG 
CAGAATGTGC AGTTCATCAA ATGCAAGCTG TGCAACGCAG AGTTTGCCTA CGAGAAGGGC 
CTGAAGGTGC ATCTGTTTAA GGTGCACGGA AAGGCCATCA AGGATGAAAT GATTATCAAG 
CAGTTCGAGT GTGACGTTTG CTCGATTGTC TATAGTTCAG AGTCGGAGCT GCAGCAGCAC 
AAACGCAGCG TTCACAAGCT GACATCCGCC TCCGCTTCCA CTTCGGCGTC CACGTCCTCC 
AAGATTGACG ACGACTCTCT AATGGATGAT GGCAAGCCGA CATCATCGGA TCTAGCTGAT 
CTCTCTACCC TCGCAGCGGG TGGATCAACT GCATCTGCTC CACTGTACTG GTACCAGTGC 
AAGTACTGTC CATCAAACTT TAACACCAAC AAGAAGCTGG CCATCCACAT CAACTCGCAT 
GACGAGTTTG ACTCGAACGA TTATTCCTGC AAAGATTGCG GAAATGTCTA CAGCGGACGC 
AAGAGTCTAT GGGTTCATCG CTATAAGAAG CATCCGCAAG TGCCCAACCC AGCTGAGTGC 
TCGCTGTGCC GCAAGGTCTT TTTCGACCGC CAGATGCACG ACAACCACAC ACCCACCTGC 
AACCGCAAGC CGATCACCTC GACAGGTGCC CACCAGCAGC AGGATGGACA ACTGCATTCG 
CACCACACGG CCAAAAGAAC AATTTTCCGG CATAAGACCG GCGATGACGA TGACGAGGAG 
GATGACGATG AGCAGCAGCA ACTGGAGGAG AGGGCAAATA GCGATGGCAA TGGCACCACT 
GTGGGAGTGG CGTCGGGCAG TACTGCAGCT GCGGGCACGT CACTAAAGAT CCGCATTCCG 
GAGGTGGCGT GCACTATTTG CGGTGCTCGC TTCACCGACC AGGAGCACTT TAGCAAGCAC 
ATCCAGAAGC ACGAGCAAGA GCTGTACGTG GACAATCCGC TGGCGGCGAT GTTCGATGAT 
GGGCCGGCGG ATGCCGGTCA GTTCCAGGTG GAGCGGCAGA ACGAGAACGG GGAATACGCG 
TGCGATTTGT GCGCCAAGAC GTTCCCCCAG GTGATCGCAC TCAAGGTGCA TCGCAAGTGG 
CATTTCAGAG GTGATAGCAA GCAGAACCCC ATCGACGGCG AAGCGACACA GTTGACCAAC 
AACAATCACA CAACCAACAA CAACAACAAC AACTCGATGC ACCTCCGCGA GCTGCATGCG 
GTGGGCCTGA TGCCCAACCA GCAGCAGCAG AGCCTCAACA ACTCGTGCAA CAGCAGCATG 
AACCACAACA ACAACAGCAG CAGCAACCGC AGCAAGTCGA TGAAGCGGAA ACGTGAGCTG 
AAATGCGAAT ACTGCGCCTC CACCTTCATC AGCAATAACA ATCTGCGTCG CCACATGTAC 
GAGCTGCACA AACACGAGGT AAGCAATCTG CCCGAGCCTC CGGTGATTGT GGTGGACGAT 
CACCTCACCT GCCGTCGCTG TCAGTTGAAG TTCGACACAA AGGAGCTGTG GATCGAGCAC 
AAGCTGGCCG ATGCAAAGGT GGTGCGACCT TTCTGCCCCT TCCAGTGGGG CTGTGATCTG 
TGCGGCGAGT ATTTGTCGCG CAAGGAGAAG CTAATGAATC ACATTAACAA CCATTTGAAG 
GAGGAGGTCA TAGTGCCAGT AGCCACTAAG GCGGCCATAG AGAGAACAGC GGCCATGGAA 
TCAGCGGCAG CAGATGCGAA TGCAGCGGCG ACACTATCAG CATTGGGCGA AGGAGCCGAA 
ACTGAAGATC AGTTTGCAGA GAAGGTTGAG GCTGCAGGGG CAACAACAAC AGATAAGTTG 
ACGAATCCCG ACGAGGAGGA TAGCGATGAT TTGGATGAGG ATAGCTCGGG CGACGACGAT 
GATAGTTCGG GGACGGGAGA TGATGACGAT GACGACGACA GCGACGATGA TGAGGATGGC 
GAGGGTGAAG ATGAGGACGA GGAGGGAGAT GGTGGCGAAG GCGAGGACGA AGAGGGTGTC 
CAGCCACCCG CTCAACTTTT GCCGCAGCAG CAACACAAAA CGGATCTTAA TCTTAACCAG 
GACGACGATG ATCTCGTCGA GGAGGTCATC AGCTCCGATG ACGACGAGGA CGATGATGGA 
GAGGTGGAAA GCGACGATGA CGACGAAGAT GATGATGATG AGGAGGACGA TGTGGAGGAG 
CCGGAGCCAG TTGGATTGAC GGTGCGACCG CTAATGAATG GCAAATCAAA GATGCCGCCG 
CTAATAGTGG CCAGTTCGGA TGATGAAGAC GATGGTGTGA TGCCCATAGA GGACATTATC 
GAAGAGGAGT TCGATGAGGA TGCCGATCCA GATCCGGAGG ATGCCATTGA GGAGGTAGAC 
GAAGATGATT TAGATGAGGG GGAAGTGGAG GACGAGCCGA ATGTGGTGTC GACGGCCTCC 
TTCTCGGAGA GCGAGTCCAG CACAACGACC ACGTCCAATT CGCATTCGCA TTCGCATTCA 
ACGGGTGAGC GGCGTAAGAA GGCCGTCGAT CAGTTGAATG ATCCCGGCTT TACCTGTGAT 
CTATGTCAAC TTTGTTTCGA TTCTCAGGAG CTTCTCCAGA GCCACATCAA AAGCCATATC 
CTCAATGGGC CAAAGCTCTC GACAGTGTCA GCAGCAGCAG CAGCAGCAGC AGCCGCCGCC 
ACAGCAAGCA GCAAGGCAAC AGCATTACTA ACGGCAGCAA AGGCGAAGCC TGACTCCAAG 
TCAGCGGTGC TGGCCAACAA TAATAACAGC AAGACAAGCA GTAAGACTGT GGCAGCAGGT 
GCGACAAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138204.1
CDS	450..7121
Translation	MCDAAAATATTTTTAAVAAAVATTTASVALEATATQPGTTTTTVATASAGTTSPEAAIPTAATATSARNSNSERSARQNCCRLCIAPQTECISIINSYAADKEPLSTKIHNCVGIKVTPQDRLSQQICHACISYLNSWQSFKNRCFSSQAKQRQWLDTNKSKLLNYLDLNSAENGGGGFFDQHLHQQQQHHQHLENELEAEKEKATPTAASTAANILDGIHSLKKRKSLTVYVSPKPQQMQQHQLQQLQLQPPARMTATISSAQQSLQTLLLQVPASAAASCSSTSTSTTSASQQREQQQHQQRQQSHQYRRYHHHHQQQQQQQQQQHNQRGKQMLPHHQQHQQQHHRRQRQFRPQRPLPVPVVNPAPTLRKFKPKLYLPLDITVPPLPPLPTIPSIPTLPPVGPGSIAPPPAAELLPGISGVAGMVESLSLSLPLDFSQSCSSSGHVQHQQAGGLATSTTTSNPPGSVVSATPSASVSASASASASVSTAPLLMPPLPAMPIKDEPIDTDDDYQMKSIDESDDMVDPTMFLERSEHEGDVPLTASDYDYTAQHGVNASSVAASLPPNAVANVAAAGDSKVASCRACSLQFSTRANARRHERNLHPNLFQLSTDSPHNTPITKPTPALAAALEMQRAAAAAATAEANRAAGAAGGNISTQKYRQVVMNAFIKCEGGGYDYDNPEQYRPLLTRDKVEFIEQNDEFLEQYQTMTCRCCNKYFSTYKNFMAHVRKKYPQLPRNLCFNCLKMNDSKALFISHLKKRNCINLFRVLNALRGKTTTVVVPIADDVADDGATGSIPVADAGAGVVAMNSPTVTASGEVVTPGGGSERPEKLRAKELLVNKLYECKLCPKGFRTKHEFRTHVYDKHADVQRKDNNSIQCSFCGLDFADPVDRRRHYNNMDCIVRLRCMTCDAKLETHQRFLDHVYQDHLGGVGGGAVSDNASTTGSGMARSNSMEHSPGKRSLLGALGVGSSAEESRSSSAAPPLTSTPKLAGGNQVGGGGSTSASAAAAAQSSANRDASAPKSQYFSRMPQVCPICGQQYNNYNNVLRHMESKHPNKLPETYKCVRCGLGYPRISYLREHMINVHGVDKNRHSGGFEYIVNADAVKLADGSTPNVYTGRYDYVMKDLMSITNGGTLDDEEEEPGSVAKKMRLDDSSNNSSLVGVASQQKECPICNAVFSNNIGLSNHMRSHYTASNAVNAALAAANRMTPKSLTITATPATDSELGVGGTMSESAPATPANVPPAMANQTPQEQAVFRRSLDQAADRRFRRMRCRICQRRFSSKKSYRYHMLTDHQVQNVQFIKCKLCNAEFAYEKGLKVHLFKVHGKAIKDEMIIKQFECDVCSIVYSSESELQQHKRSVHKLTSASASTSASTSSKIDDDSLMDDGKPTSSDLADLSTLAAGGSTASAPLYWYQCKYCPSNFNTNKKLAIHINSHDEFDSNDYSCKDCGNVYSGRKSLWVHRYKKHPQVPNPAECSLCRKVFFDRQMHDNHTPTCNRKPITSTGAHQQQDGQLHSHHTAKRTIFRHKTGDDDDEEDDDEQQQLEERANSDGNGTTVGVASGSTAAAGTSLKIRIPEVACTICGARFTDQEHFSKHIQKHEQELYVDNPLAAMFDDGPADAGQFQVERQNENGEYACDLCAKTFPQVIALKVHRKWHFRGDSKQNPIDGEATQLTNNNHTTNNNNNNSMHLRELHAVGLMPNQQQQSLNNSCNSSMNHNNNSSSNRSKSMKRKRELKCEYCASTFISNNNLRRHMYELHKHEVSNLPEPPVIVVDDHLTCRRCQLKFDTKELWIEHKLADAKVVRPFCPFQWGCDLCGEYLSRKEKLMNHINNHLKEEVIVPVATKAAIERTAAMESAAADANAAATLSALGEGAETEDQFAEKVEAAGATTTDKLTNPDEEDSDDLDEDSSGDDDDSSGTGDDDDDDDSDDDEDGEGEDEDEEGDGGEGEDEEGVQPPAQLLPQQQHKTDLNLNQDDDDLVEEVISSDDDEDDDGEVESDDDDEDDDDEEDDVEEPEPVGLTVRPLMNGKSKMPPLIVASSDDEDDGVMPIEDIIEEEFDEDADPDPEDAIEEVDEDDLDEGEVEDEPNVVSTASFSESESSTTTTSNSHSHSHSTGERRKKAVDQLNDPGFTCDLCQLCFDSQELLQSHIKSHILNGPKLSTVSAAAAAAAAAATASSKATALLTAAKAKPDSKSAVLANNNNSKTSSKTVAAGATN

Target ORF information:

RefSeq Version	NM_001144732.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster hangover (hang), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144732.2

ORF Insert Sequence:

ATGTGCGACG CTGCGGCGGC AACAGCGACA ACAACAACAA CAGCAGCAGT AGCAGCAGCC 
GTCGCAACAA CAACAGCATC GGTAGCATTG GAGGCCACAG CGACACAGCC TGGAACGACA 
ACGACGACGG TAGCCACTGC GTCAGCGGGA ACCACATCCC CTGAAGCGGC CATTCCAACA 
GCAGCAACAG CAACATCGGC GAGAAACAGC AACAGCGAAC GGTCGGCGCG ACAAAACTGC 
TGTCGCCTGT GCATCGCCCC ACAAACCGAA TGCATCTCGA TCATCAATAG CTATGCGGCC 
GACAAGGAGC CGCTGTCCAC CAAGATCCAC AACTGCGTCG GCATCAAGGT TACACCGCAG 
GATCGGCTGT CGCAGCAAAT TTGCCACGCC TGCATCAGTT ACCTGAACTC ATGGCAGAGC 
TTCAAGAACC GGTGCTTCAG CTCGCAAGCG AAGCAGCGCC AGTGGCTGGA TACCAACAAG 
AGCAAGCTCC TCAACTACCT GGACCTGAAC AGTGCCGAGA ATGGGGGAGG AGGCTTCTTC 
GATCAGCATC TGCATCAACA ACAGCAACAC CACCAGCACT TGGAGAATGA GCTCGAAGCG 
GAGAAGGAGA AGGCGACGCC AACGGCCGCA TCGACAGCCG CCAACATATT GGACGGCATA 
CACTCGCTGA AGAAACGCAA ATCCCTAACA GTCTATGTGA GTCCTAAACC GCAGCAGATG 
CAACAGCATC AATTGCAGCA GTTGCAGCTG CAACCACCGG CCCGAATGAC CGCGACGATC 
AGCAGTGCTC AGCAATCGCT GCAGACCCTT CTCTTGCAGG TACCAGCATC GGCGGCGGCT 
AGCTGCTCAT CCACATCCAC GTCCACCACA TCCGCATCCC AGCAGCGGGA ACAGCAGCAG 
CATCAGCAGC GCCAGCAATC TCACCAGTAT CGCAGATACC ATCATCATCA TCAGCAGCAG 
CAGCAACAGC AGCAGCAGCA GCATAATCAG CGTGGCAAGC AAATGTTGCC ACATCATCAG 
CAGCATCAGC AACAGCACCA CAGACGCCAG CGACAATTTC GCCCACAGCG TCCACTTCCA 
GTTCCGGTGG TCAATCCGGC TCCAACATTG CGCAAATTTA AGCCAAAGCT ATATTTGCCA 
TTGGACATAA CAGTTCCTCC GTTGCCACCG CTGCCAACTA TACCATCGAT TCCAACATTG 
CCCCCAGTGG GCCCGGGATC CATTGCTCCG CCTCCTGCTG CTGAACTGCT GCCGGGAATT 
TCGGGTGTGG CTGGAATGGT GGAGAGCCTG TCGCTGTCGC TGCCGCTTGA TTTCAGCCAG 
AGCTGCAGCA GCAGCGGTCA CGTCCAACAT CAGCAGGCTG GTGGACTGGC CACGTCGACG 
ACCACGTCCA ATCCGCCGGG CAGCGTCGTT TCCGCCACGC CTTCTGCATC TGTATCTGCT 
TCTGCTTCTG CTTCCGCATC CGTATCAACG GCTCCGCTGC TTATGCCGCC ACTGCCTGCG 
ATGCCCATCA AAGATGAGCC GATTGATACG GATGACGACT ATCAGATGAA GTCCATAGAC 
GAGTCCGATG ACATGGTCGA TCCCACAATG TTCCTAGAGC GCTCCGAGCA CGAGGGTGAT 
GTCCCGCTGA CGGCCTCGGA TTACGACTAC ACTGCGCAGC ATGGCGTGAA TGCTTCGTCC 
GTGGCTGCCT CGCTGCCGCC GAATGCGGTG GCCAATGTGG CAGCCGCCGG GGACTCCAAG 
GTGGCCAGCT GCAGGGCCTG CAGCCTGCAG TTCTCTACCC GGGCCAACGC CCGTCGCCAC 
GAAAGGAATC TACACCCAAA CCTGTTCCAG TTGTCCACAG ACTCCCCCCA CAACACACCC 
ATCACAAAGC CAACGCCCGC TTTGGCTGCC GCCTTGGAAA TGCAACGGGC AGCGGCTGCG 
GCGGCCACCG CTGAGGCAAA TCGGGCAGCG GGCGCCGCTG GCGGGAATAT CTCCACGCAA 
AAGTACCGCC AGGTGGTGAT GAACGCGTTC ATTAAGTGCG AGGGCGGCGG CTACGACTAC 
GATAATCCCG AACAGTACCG ACCATTGCTC ACCCGCGACA AAGTGGAGTT CATTGAGCAG 
AACGATGAGT TCCTCGAGCA ATACCAGACG ATGACCTGCC GATGTTGCAA CAAATACTTC 
AGCACGTACA AGAACTTCAT GGCGCACGTT CGCAAGAAGT ATCCGCAGCT GCCGCGCAAC 
CTATGCTTCA ATTGCCTCAA GATGAACGAC TCCAAGGCGC TGTTCATCTC GCATCTGAAG 
AAGCGGAATT GCATAAATCT CTTTAGAGTC TTGAATGCGT TGCGTGGGAA AACAACGACA 
GTGGTTGTGC CCATTGCAGA TGATGTGGCA GACGATGGAG CAACAGGATC TATTCCAGTT 
GCCGATGCCG GAGCAGGAGT GGTGGCCATG AATAGTCCCA CAGTGACAGC CAGCGGAGAA 
GTTGTTACAC CCGGCGGCGG CTCCGAGCGC CCAGAGAAAC TGCGCGCCAA GGAACTTCTG 
GTCAACAAAC TGTACGAGTG CAAGCTCTGT CCCAAGGGAT TCCGCACTAA GCACGAGTTC 
CGAACGCATG TCTACGACAA GCACGCAGAT GTCCAACGCA AGGATAACAA CTCGATACAG 
TGCAGCTTTT GCGGACTGGA CTTTGCCGAT CCCGTTGACA GACGGCGTCA CTACAACAAC 
ATGGACTGCA TTGTCCGGCT GCGCTGCATG ACGTGCGATG CCAAGCTGGA AACGCACCAG 
CGATTCCTCG ATCATGTCTA CCAGGATCAC TTGGGCGGTG TGGGTGGTGG CGCGGTCAGC 
GACAACGCCT CCACCACTGG CAGCGGCATG GCCAGGAGCA ACAGCATGGA ACACTCGCCG 
GGCAAAAGGA GCCTGCTCGG CGCCTTAGGC GTTGGTTCCT CGGCGGAGGA GTCGCGAAGC 
AGCAGTGCGG CTCCGCCGCT GACCTCTACA CCAAAACTGG CGGGCGGTAA TCAGGTGGGT 
GGCGGTGGAT CGACCAGCGC TTCTGCCGCC GCAGCCGCTC AGAGCTCGGC GAATCGGGAT 
GCATCCGCAC CCAAATCCCA GTACTTCTCC CGTATGCCGC AGGTTTGTCC CATTTGCGGC 
CAGCAGTACA ACAACTACAA CAATGTGCTG CGCCACATGG AATCGAAGCA TCCGAACAAA 
CTGCCAGAGA CATACAAGTG CGTGCGCTGC GGACTGGGCT ATCCCCGGAT CTCCTACCTA 
CGGGAGCACA TGATCAATGT GCACGGAGTG GACAAGAACC GTCACTCTGG CGGCTTCGAA 
TACATCGTGA ATGCGGATGC CGTGAAGTTG GCGGACGGAA GCACGCCGAA CGTCTACACG 
GGTCGCTACG ATTACGTGAT GAAGGACCTG ATGTCGATAA CAAATGGTGG GACACTCGAT 
GATGAAGAGG AGGAGCCTGG CAGCGTGGCC AAGAAGATGC GCCTGGATGA CAGTAGCAAC 
AACAGCAGCC TGGTGGGCGT GGCTAGCCAG CAGAAGGAGT GCCCCATCTG CAATGCGGTG 
TTCAGCAACA ACATTGGCCT GTCCAATCAT ATGCGTTCCC ACTATACGGC CTCGAATGCA 
GTGAATGCAG CCTTGGCGGC TGCCAATCGA ATGACACCCA AATCGCTAAC GATAACCGCA 
ACGCCAGCAA CGGATTCGGA GTTGGGAGTT GGAGGAACAA TGTCCGAGTC GGCTCCAGCA 
ACGCCTGCCA ATGTGCCACC GGCAATGGCT AACCAAACGC CCCAGGAGCA GGCAGTTTTT 
CGCCGGAGCC TTGACCAGGC AGCCGATCGC CGCTTCCGGC GAATGCGATG CCGCATCTGC 
CAGCGTCGCT TCAGTTCGAA GAAGTCCTAT CGCTACCACA TGCTCACCGA TCATCAGGTG 
CAGAATGTGC AGTTCATCAA ATGCAAGCTG TGCAACGCAG AGTTTGCCTA CGAGAAGGGC 
CTGAAGGTGC ATCTGTTTAA GGTGCACGGA AAGGCCATCA AGGATGAAAT GATTATCAAG 
CAGTTCGAGT GTGACGTTTG CTCGATTGTC TATAGTTCAG AGTCGGAGCT GCAGCAGCAC 
AAACGCAGCG TTCACAAGCT GACATCCGCC TCCGCTTCCA CTTCGGCGTC CACGTCCTCC 
AAGATTGACG ACGACTCTCT AATGGATGAT GGCAAGCCGA CATCATCGGA TCTAGCTGAT 
CTCTCTACCC TCGCAGCGGG TGGATCAACT GCATCTGCTC CACTGTACTG GTACCAGTGC 
AAGTACTGTC CATCAAACTT TAACACCAAC AAGAAGCTGG CCATCCACAT CAACTCGCAT 
GACGAGTTTG ACTCGAACGA TTATTCCTGC AAAGATTGCG GAAATGTCTA CAGCGGACGC 
AAGAGTCTAT GGGTTCATCG CTATAAGAAG CATCCGCAAG TGCCCAACCC AGCTGAGTGC 
TCGCTGTGCC GCAAGGTCTT TTTCGACCGC CAGATGCACG ACAACCACAC ACCCACCTGC 
AACCGCAAGC CGATCACCTC GACAGGTGCC CACCAGCAGC AGGATGGACA ACTGCATTCG 
CACCACACGG CCAAAAGAAC AATTTTCCGG CATAAGACCG GCGATGACGA TGACGAGGAG 
GATGACGATG AGCAGCAGCA ACTGGAGGAG AGGGCAAATA GCGATGGCAA TGGCACCACT 
GTGGGAGTGG CGTCGGGCAG TACTGCAGCT GCGGGCACGT CACTAAAGAT CCGCATTCCG 
GAGGTGGCGT GCACTATTTG CGGTGCTCGC TTCACCGACC AGGAGCACTT TAGCAAGCAC 
ATCCAGAAGC ACGAGCAAGA GCTGTACGTG GACAATCCGC TGGCGGCGAT GTTCGATGAT 
GGGCCGGCGG ATGCCGGTCA GTTCCAGGTG GAGCGGCAGA ACGAGAACGG GGAATACGCG 
TGCGATTTGT GCGCCAAGAC GTTCCCCCAG GTGATCGCAC TCAAGGTGCA TCGCAAGTGG 
CATTTCAGAG GTGATAGCAA GCAGAACCCC ATCGACGGCG AAGCGACACA GTTGACCAAC 
AACAATCACA CAACCAACAA CAACAACAAC AACTCGATGC ACCTCCGCGA GCTGCATGCG 
GTGGGCCTGA TGCCCAACCA GCAGCAGCAG AGCCTCAACA ACTCGTGCAA CAGCAGCATG 
AACCACAACA ACAACAGCAG CAGCAACCGC AGCAAGTCGA TGAAGCGGAA ACGTGAGCTG 
AAATGCGAAT ACTGCGCCTC CACCTTCATC AGCAATAACA ATCTGCGTCG CCACATGTAC 
GAGCTGCACA AACACGAGGT AAGCAATCTG CCCGAGCCTC CGGTGATTGT GGTGGACGAT 
CACCTCACCT GCCGTCGCTG TCAGTTGAAG TTCGACACAA AGGAGCTGTG GATCGAGCAC 
AAGCTGGCCG ATGCAAAGGT GGTGCGACCT TTCTGCCCCT TCCAGTGGGG CTGTGATCTG 
TGCGGCGAGT ATTTGTCGCG CAAGGAGAAG CTAATGAATC ACATTAACAA CCATTTGAAG 
GAGGAGGTCA TAGTGCCAGT AGCCACTAAG GCGGCCATAG AGAGAACAGC GGCCATGGAA 
TCAGCGGCAG CAGATGCGAA TGCAGCGGCG ACACTATCAG CATTGGGCGA AGGAGCCGAA 
ACTGAAGATC AGTTTGCAGA GAAGGTTGAG GCTGCAGGGG CAACAACAAC AGATAAGTTG 
ACGAATCCCG ACGAGGAGGA TAGCGATGAT TTGGATGAGG ATAGCTCGGG CGACGACGAT 
GATAGTTCGG GGACGGGAGA TGATGACGAT GACGACGACA GCGACGATGA TGAGGATGGC 
GAGGGTGAAG ATGAGGACGA GGAGGGAGAT GGTGGCGAAG GCGAGGACGA AGAGGGTGTC 
CAGCCACCCG CTCAACTTTT GCCGCAGCAG CAACACAAAA CGGATCTTAA TCTTAACCAG 
GACGACGATG ATCTCGTCGA GGAGGTCATC AGCTCCGATG ACGACGAGGA CGATGATGGA 
GAGGTGGAAA GCGACGATGA CGACGAAGAT GATGATGATG AGGAGGACGA TGTGGAGGAG 
CCGGAGCCAG TTGGATTGAC GGTGCGACCG CTAATGAATG GCAAATCAAA GATGCCGCCG 
CTAATAGTGG CCAGTTCGGA TGATGAAGAC GATGGTGTGA TGCCCATAGA GGACATTATC 
GAAGAGGAGT TCGATGAGGA TGCCGATCCA GATCCGGAGG ATGCCATTGA GGAGGTAGAC 
GAAGATGATT TAGATGAGGG GGAAGTGGAG GACGAGCCGA ATGTGGTGTC GACGGCCTCC 
TTCTCGGAGA GCGAGTCCAG CACAACGACC ACGTCCAATT CGCATTCGCA TTCGCATTCA 
ACGGGTGAGC GGCGTAAGAA GGCCGTCGAT CAGTTGAATG ATCCCGGCTT TACCTGTGAT 
CTATGTCAAC TTTGTTTCGA TTCTCAGGAG CTTCTCCAGA GCCACATCAA AAGCCATATC 
CTCAATGGGC CAAAGCTCTC GACAGTGTCA GCAGCAGCAG CAGCAGCAGC AGCCGCCGCC 
ACAGCAAGCA GCAAGGCAAC AGCATTACTA ACGGCAGCAA AGGCGAAGCC TGACTCCAAG 
TCAGCGGTGC TGGCCAACAA TAATAACAGC AAGACAAGCA GTAAGACTGT GGCAGCAGGT 
GCGACAAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hang ( NM_167521.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727980.2 Drosophila melanogaster hangover (hang), transcript variant A, mRNA.

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CloneID	OFa01801
Clone ID Related Accession (Same CDS sequence)	NM_167521.3
Accession Version	NM_167521.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5706bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	hangover, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167521.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGCGACG CTGCGGCGGC AACAGCGACA ACAACAACAA CAGCAGCAGT AGCAGCAGCC 
GTCGCAACAA CAACAGCATC GGTAGCATTG GAGGCCACAG CGACACAGCC TGGAACGACA 
ACGACGACGG TAGCCACTGC GTCAGCGGGA ACCACATCCC CTGAAGCGGC CATTCCAACA 
GCAGCAACAG CAACATCGGC GAGAAACAGC AACAGCGAAC GGTCGGCGCG ACAAAACTGC 
TGTCGCCTGT GCATCGCCCC ACAAACCGAA TGCATCTCGA TCATCAATAG CTATGCGGCC 
GACAAGGAGC CGCTGTCCAC CAAGATCCAC AACTGCGTCG GCATCAAGGT TACACCGCAG 
GATCGGCTGT CGCAGCAAAT TTGCCACGCC TGCATCAGTT ACCTGAACTC ATGGCAGAGC 
TTCAAGAACC GGTGCTTCAG CTCGCAAGCG AAGCAGCGCC AGTGGCTGGA TACCAACAAG 
AGCAAGCTCC TCAACTACCT GGACCTGAAC AGTGCCGAGA ATGGGGGAGG AGGCTTCTTC 
GATCAGCATC TGCATCAACA ACAGCAACAC CACCAGCACT TGGAGAATGA GCTCGAAGCG 
GAGAAGGAGA AGGCGACGCC AACGGCCGCA TCGACAGCCG CCAACATATT GGACGGCATA 
CACTCGCTGA AGAAACGCAA ATCCCTAACA GTCTATCCAC TGCCTGCGAT GCCCATCAAA 
GATGAGCCGA TTGATACGGA TGACGACTAT CAGATGAAGT CCATAGACGA GTCCGATGAC 
ATGGTCGATC CCACAATGTT CCTAGAGCGC TCCGAGCACG AGGGTGATGT CCCGCTGACG 
GCCTCGGATT ACGACTACAC TGCGCAGCAT GGCGTGAATG CTTCGTCCGT GGCTGCCTCG 
CTGCCGCCGA ATGCGGTGGC CAATGTGGCA GCCGCCGGGG ACTCCAAGGT GGCCAGCTGC 
AGGGCCTGCA GCCTGCAGTT CTCTACCCGG GCCAACGCCC GTCGCCACGA AAGGAATCTA 
CACCCAAACC TGTTCCAGTT GTCCACAGAC TCCCCCCACA ACACACCCAT CACAAAGCCA 
ACGCCCGCTT TGGCTGCCGC CTTGGAAATG CAACGGGCAG CGGCTGCGGC GGCCACCGCT 
GAGGCAAATC GGGCAGCGGG CGCCGCTGGC GGGAATATCT CCACGCAAAA GTACCGCCAG 
GTGGTGATGA ACGCGTTCAT TAAGTGCGAG GGCGGCGGCT ACGACTACGA TAATCCCGAA 
CAGTACCGAC CATTGCTCAC CCGCGACAAA GTGGAGTTCA TTGAGCAGAA CGATGAGTTC 
CTCGAGCAAT ACCAGACGAT GACCTGCCGA TGTTGCAACA AATACTTCAG CACGTACAAG 
AACTTCATGG CGCACGTTCG CAAGAAGTAT CCGCAGCTGC CGCGCAACCT ATGCTTCAAT 
TGCCTCAAGA TGAACGACTC CAAGGCGCTG TTCATCTCGC ATCTGAAGAA GCGGAATTGC 
ATAAATCTCT TTAGAGTCTT GAATGCGTTG CGTGGGAAAA CAACGACAGT GGTTGTGCCC 
ATTGCAGATG ATGTGGCAGA CGATGGAGCA ACAGGATCTA TTCCAGTTGC CGATGCCGGA 
GCAGGAGTGG TGGCCATGAA TAGTCCCACA GTGACAGCCA GCGGAGAAGT TGTTACACCC 
GGCGGCGGCT CCGAGCGCCC AGAGAAACTG CGCGCCAAGG AACTTCTGGT CAACAAACTG 
TACGAGTGCA AGCTCTGTCC CAAGGGATTC CGCACTAAGC ACGAGTTCCG AACGCATGTC 
TACGACAAGC ACGCAGATGT CCAACGCAAG GATAACAACT CGATACAGTG CAGCTTTTGC 
GGACTGGACT TTGCCGATCC CGTTGACAGA CGGCGTCACT ACAACAACAT GGACTGCATT 
GTCCGGCTGC GCTGCATGAC GTGCGATGCC AAGCTGGAAA CGCACCAGCG ATTCCTCGAT 
CATGTCTACC AGGATCACTT GGGCGGTGTG GGTGGTGGCG CGGTCAGCGA CAACGCCTCC 
ACCACTGGCA GCGGCATGGC CAGGAGCAAC AGCATGGAAC ACTCGCCGGG CAAAAGGAGC 
CTGCTCGGCG CCTTAGGCGT TGGTTCCTCG GCGGAGGAGT CGCGAAGCAG CAGTGCGGCT 
CCGCCGCTGA CCTCTACACC AAAACTGGCG GGCGGTAATC AGGTGGGTGG CGGTGGATCG 
ACCAGCGCTT CTGCCGCCGC AGCCGCTCAG AGCTCGGCGA ATCGGGATGC ATCCGCACCC 
AAATCCCAGT ACTTCTCCCG TATGCCGCAG GTTTGTCCCA TTTGCGGCCA GCAGTACAAC 
AACTACAACA ATGTGCTGCG CCACATGGAA TCGAAGCATC CGAACAAACT GCCAGAGACA 
TACAAGTGCG TGCGCTGCGG ACTGGGCTAT CCCCGGATCT CCTACCTACG GGAGCACATG 
ATCAATGTGC ACGGAGTGGA CAAGAACCGT CACTCTGGCG GCTTCGAATA CATCGTGAAT 
GCGGATGCCG TGAAGTTGGC GGACGGAAGC ACGCCGAACG TCTACACGGG TCGCTACGAT 
TACGTGATGA AGGACCTGAT GTCGATAACA AATGATGATG AAGAGGAGGA GCCTGGCAGC 
GTGGCCAAGA AGATGCGCCT GGATGACAGT AGCAACAACA GCAGCCTGGT GGGCGTGGCT 
AGCCAGCAGA AGGAGTGCCC CATCTGCAAT GCGGTGTTCA GCAACAACAT TGGCCTGTCC 
AATCATATGC GTTCCCACTA TACGGCCTCG AATGCAGTGA ATGCAGCCTT GGCGGCTGCC 
AATCGAATGA CACCCAAATC GCTAACGATA ACCGCAACGC CAGCAACGGA TTCGGAGTTG 
GGAGTTGGAG GAACAATGTC CGAGTCGGCT CCAGCAACGC CTGCCAATGT GCCACCGGCA 
ATGGCTAACC AAACGCCCCA GGAGCAGGCA GTTTTTCGCC GGAGCCTTGA CCAGGCAGCC 
GATCGCCGCT TCCGGCGAAT GCGATGCCGC ATCTGCCAGC GTCGCTTCAG TTCGAAGAAG 
TCCTATCGCT ACCACATGCT CACCGATCAT CAGGTGCAGA ATGTGCAGTT CATCAAATGC 
AAGCTGTGCA ACGCAGAGTT TGCCTACGAG AAGGGCCTGA AGGTGCATCT GTTTAAGGTG 
CACGGAAAGG CCATCAAGGA TGAAATGATT ATCAAGCAGT TCGAGTGTGA CGTTTGCTCG 
ATTGTCTATA GTTCAGAGTC GGAGCTGCAG CAGCACAAAC GCAGCGTTCA CAAGCTGACA 
TCCGCCTCCG CTTCCACTTC GGCGTCCACG TCCTCCAAGA TTGACGACGA CTCTCTAATG 
GATGATGGCA AGCCGACATC ATCGGATCTA GCTGATCTCT CTACCCTCGC AGCGGGTGGA 
TCAACTGCAT CTGCTCCACT GTACTGGTAC CAGTGCAAGT ACTGTCCATC AAACTTTAAC 
ACCAACAAGA AGCTGGCCAT CCACATCAAC TCGCATGACG AGTTTGACTC GAACGATTAT 
TCCTGCAAAG ATTGCGGAAA TGTCTACAGC GGACGCAAGA GTCTATGGGT TCATCGCTAT 
AAGAAGCATC CGCAAGTGCC CAACCCAGCT GAGTGCTCGC TGTGCCGCAA GGTCTTTTTC 
GACCGCCAGA TGCACGACAA CCACACACCC ACCTGCAACC GCAAGCCGAT CACCTCGACA 
GGTGCCCACC AGCAGCAGGA TGGACAACTG CATTCGCACC ACACGGCCAA AAGAACAATT 
TTCCGGCATA AGACCGGCGA TGACGATGAC GAGGAGGATG ACGATGAGCA GCAGCAACTG 
GAGGAGAGGG CAAATAGCGA TGGCAATGGC ACCACTGTGG GAGTGGCGTC GGGCAGTACT 
GCAGCTGCGG GCACGTCACT AAAGATCCGC ATTCCGGAGG TGGCGTGCAC TATTTGCGGT 
GCTCGCTTCA CCGACCAGGA GCACTTTAGC AAGCACATCC AGAAGCACGA GCAAGAGCTG 
TACGTGGACA ATCCGCTGGC GGCGATGTTC GATGATGGGC CGGCGGATGC CGGTCAGTTC 
CAGGTGGAGC GGCAGAACGA GAACGGGGAA TACGCGTGCG ATTTGTGCGC CAAGACGTTC 
CCCCAGGTGA TCGCACTCAA GGTGCATCGC AAGTGGCATT TCAGAGGTGA TAGCAAGCAG 
AACCCCATCG ACGGCGAAGC GACACAGTTG ACCAACAACA ATCACACAAC CAACAACAAC 
AACAACAACT CGATGCACCT CCGCGAGCTG CATGCGGTGG GCCTGATGCC CAACCAGCAG 
CAGCAGAGCC TCAACAACTC GTGCAACAGC AGCATGAACC ACAACAACAA CAGCAGCAGC 
AACCGCAGCA AGTCGATGAA GCGGAAACGT GAGCTGAAAT GCGAATACTG CGCCTCCACC 
TTCATCAGCA ATAACAATCT GCGTCGCCAC ATGTACGAGC TGCACAAACA CGAGGTAAGC 
AATCTGCCCG AGCCTCCGGT GATTGTGGTG GACGATCACC TCACCTGCCG TCGCTGTCAG 
TTGAAGTTCG ACACAAAGGA GCTGTGGATC GAGCACAAGC TGGCCGATGC AAAGGTGGTG 
CGACCTTTCT GCCCCTTCCA GTGGGGCTGT GATCTGTGCG GCGAGTATTT GTCGCGCAAG 
GAGAAGCTAA TGAATCACAT TAACAACCAT TTGAAGGAGG AGGTCATAGT GCCAGTAGCC 
ACTAAGGCGG CCATAGAGAG AACAGCGGCC ATGGAATCAG CGGCAGCAGA TGCGAATGCA 
GCGGCGACAC TATCAGCATT GGGCGAAGGA GCCGAAACTG AAGATCAGTT TGCAGAGAAG 
GTTGAGGCTG CAGGGGCAAC AACAACAGAT AAGTTGACGA ATCCCGACGA GGAGGATAGC 
GATGATTTGG ATGAGGATAG CTCGGGCGAC GACGATGATA GTTCGGGGAC GGGAGATGAT 
GACGATGACG ACGACAGCGA CGATGATGAG GATGGCGAGG GTGAAGATGA GGACGAGGAG 
GGAGATGGTG GCGAAGGCGA GGACGAAGAG GGTGTCCAGC CACCCGCTCA ACTTTTGCCG 
CAGCAGCAAC ACAAAACGGA TCTTAATCTT AACCAGGACG ACGATGATCT CGTCGAGGAG 
GTCATCAGCT CCGATGACGA CGAGGACGAT GATGGAGAGG TGGAAAGCGA CGATGACGAC 
GAAGATGATG ATGATGAGGA GGACGATGTG GAGGAGCCGG AGCCAGTTGG ATTGACGGTG 
CGACCGCTAA TGAATGGCAA ATCAAAGATG CCGCCGCTAA TAGTGGCCAG TTCGGATGAT 
GAAGACGATG GTGTGATGCC CATAGAGGAC ATTATCGAAG AGGAGTTCGA TGAGGATGCC 
GATCCAGATC CGGAGGATGC CATTGAGGAG GTAGACGAAG ATGATTTAGA TGAGGGGGAA 
GTGGAGGACG AGCCGAATGT GGTGTCGACG GCCTCCTTCT CGGAGAGCGA GTCCAGCACA 
ACGACCACGT CCAATTCGCA TTCGCATTCG CATTCAACGG GAGCTTCTCC AGAGCCACAT 
CAAAAGCCAT ATCCTCAATG GGCCAAAGCT CTCGACAGTG TCAGCAGCAG CAGCAGCAGC 
AGCAGCCGCC GCCACAGCAA GCAGCAAGGC AACAGCATTA CTAACGGCAG CAAAGGCGAA 
GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727980.2
CDS	450..6155
Translation	MCDAAAATATTTTTAAVAAAVATTTASVALEATATQPGTTTTTVATASAGTTSPEAAIPTAATATSARNSNSERSARQNCCRLCIAPQTECISIINSYAADKEPLSTKIHNCVGIKVTPQDRLSQQICHACISYLNSWQSFKNRCFSSQAKQRQWLDTNKSKLLNYLDLNSAENGGGGFFDQHLHQQQQHHQHLENELEAEKEKATPTAASTAANILDGIHSLKKRKSLTVYPLPAMPIKDEPIDTDDDYQMKSIDESDDMVDPTMFLERSEHEGDVPLTASDYDYTAQHGVNASSVAASLPPNAVANVAAAGDSKVASCRACSLQFSTRANARRHERNLHPNLFQLSTDSPHNTPITKPTPALAAALEMQRAAAAAATAEANRAAGAAGGNISTQKYRQVVMNAFIKCEGGGYDYDNPEQYRPLLTRDKVEFIEQNDEFLEQYQTMTCRCCNKYFSTYKNFMAHVRKKYPQLPRNLCFNCLKMNDSKALFISHLKKRNCINLFRVLNALRGKTTTVVVPIADDVADDGATGSIPVADAGAGVVAMNSPTVTASGEVVTPGGGSERPEKLRAKELLVNKLYECKLCPKGFRTKHEFRTHVYDKHADVQRKDNNSIQCSFCGLDFADPVDRRRHYNNMDCIVRLRCMTCDAKLETHQRFLDHVYQDHLGGVGGGAVSDNASTTGSGMARSNSMEHSPGKRSLLGALGVGSSAEESRSSSAAPPLTSTPKLAGGNQVGGGGSTSASAAAAAQSSANRDASAPKSQYFSRMPQVCPICGQQYNNYNNVLRHMESKHPNKLPETYKCVRCGLGYPRISYLREHMINVHGVDKNRHSGGFEYIVNADAVKLADGSTPNVYTGRYDYVMKDLMSITNDDEEEEPGSVAKKMRLDDSSNNSSLVGVASQQKECPICNAVFSNNIGLSNHMRSHYTASNAVNAALAAANRMTPKSLTITATPATDSELGVGGTMSESAPATPANVPPAMANQTPQEQAVFRRSLDQAADRRFRRMRCRICQRRFSSKKSYRYHMLTDHQVQNVQFIKCKLCNAEFAYEKGLKVHLFKVHGKAIKDEMIIKQFECDVCSIVYSSESELQQHKRSVHKLTSASASTSASTSSKIDDDSLMDDGKPTSSDLADLSTLAAGGSTASAPLYWYQCKYCPSNFNTNKKLAIHINSHDEFDSNDYSCKDCGNVYSGRKSLWVHRYKKHPQVPNPAECSLCRKVFFDRQMHDNHTPTCNRKPITSTGAHQQQDGQLHSHHTAKRTIFRHKTGDDDDEEDDDEQQQLEERANSDGNGTTVGVASGSTAAAGTSLKIRIPEVACTICGARFTDQEHFSKHIQKHEQELYVDNPLAAMFDDGPADAGQFQVERQNENGEYACDLCAKTFPQVIALKVHRKWHFRGDSKQNPIDGEATQLTNNNHTTNNNNNNSMHLRELHAVGLMPNQQQQSLNNSCNSSMNHNNNSSSNRSKSMKRKRELKCEYCASTFISNNNLRRHMYELHKHEVSNLPEPPVIVVDDHLTCRRCQLKFDTKELWIEHKLADAKVVRPFCPFQWGCDLCGEYLSRKEKLMNHINNHLKEEVIVPVATKAAIERTAAMESAAADANAAATLSALGEGAETEDQFAEKVEAAGATTTDKLTNPDEEDSDDLDEDSSGDDDDSSGTGDDDDDDDSDDDEDGEGEDEDEEGDGGEGEDEEGVQPPAQLLPQQQHKTDLNLNQDDDDLVEEVISSDDDEDDDGEVESDDDDEDDDDEEDDVEEPEPVGLTVRPLMNGKSKMPPLIVASSDDEDDGVMPIEDIIEEEFDEDADPDPEDAIEEVDEDDLDEGEVEDEPNVVSTASFSESESSTTTTSNSHSHSHSTGASPEPHQKPYPQWAKALDSVSSSSSSSSRRHSKQQGNSITNGSKGEA

Target ORF information:

RefSeq Version	NM_167521.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster hangover (hang), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167521.3

ORF Insert Sequence:

ATGTGCGACG CTGCGGCGGC AACAGCGACA ACAACAACAA CAGCAGCAGT AGCAGCAGCC 
GTCGCAACAA CAACAGCATC GGTAGCATTG GAGGCCACAG CGACACAGCC TGGAACGACA 
ACGACGACGG TAGCCACTGC GTCAGCGGGA ACCACATCCC CTGAAGCGGC CATTCCAACA 
GCAGCAACAG CAACATCGGC GAGAAACAGC AACAGCGAAC GGTCGGCGCG ACAAAACTGC 
TGTCGCCTGT GCATCGCCCC ACAAACCGAA TGCATCTCGA TCATCAATAG CTATGCGGCC 
GACAAGGAGC CGCTGTCCAC CAAGATCCAC AACTGCGTCG GCATCAAGGT TACACCGCAG 
GATCGGCTGT CGCAGCAAAT TTGCCACGCC TGCATCAGTT ACCTGAACTC ATGGCAGAGC 
TTCAAGAACC GGTGCTTCAG CTCGCAAGCG AAGCAGCGCC AGTGGCTGGA TACCAACAAG 
AGCAAGCTCC TCAACTACCT GGACCTGAAC AGTGCCGAGA ATGGGGGAGG AGGCTTCTTC 
GATCAGCATC TGCATCAACA ACAGCAACAC CACCAGCACT TGGAGAATGA GCTCGAAGCG 
GAGAAGGAGA AGGCGACGCC AACGGCCGCA TCGACAGCCG CCAACATATT GGACGGCATA 
CACTCGCTGA AGAAACGCAA ATCCCTAACA GTCTATCCAC TGCCTGCGAT GCCCATCAAA 
GATGAGCCGA TTGATACGGA TGACGACTAT CAGATGAAGT CCATAGACGA GTCCGATGAC 
ATGGTCGATC CCACAATGTT CCTAGAGCGC TCCGAGCACG AGGGTGATGT CCCGCTGACG 
GCCTCGGATT ACGACTACAC TGCGCAGCAT GGCGTGAATG CTTCGTCCGT GGCTGCCTCG 
CTGCCGCCGA ATGCGGTGGC CAATGTGGCA GCCGCCGGGG ACTCCAAGGT GGCCAGCTGC 
AGGGCCTGCA GCCTGCAGTT CTCTACCCGG GCCAACGCCC GTCGCCACGA AAGGAATCTA 
CACCCAAACC TGTTCCAGTT GTCCACAGAC TCCCCCCACA ACACACCCAT CACAAAGCCA 
ACGCCCGCTT TGGCTGCCGC CTTGGAAATG CAACGGGCAG CGGCTGCGGC GGCCACCGCT 
GAGGCAAATC GGGCAGCGGG CGCCGCTGGC GGGAATATCT CCACGCAAAA GTACCGCCAG 
GTGGTGATGA ACGCGTTCAT TAAGTGCGAG GGCGGCGGCT ACGACTACGA TAATCCCGAA 
CAGTACCGAC CATTGCTCAC CCGCGACAAA GTGGAGTTCA TTGAGCAGAA CGATGAGTTC 
CTCGAGCAAT ACCAGACGAT GACCTGCCGA TGTTGCAACA AATACTTCAG CACGTACAAG 
AACTTCATGG CGCACGTTCG CAAGAAGTAT CCGCAGCTGC CGCGCAACCT ATGCTTCAAT 
TGCCTCAAGA TGAACGACTC CAAGGCGCTG TTCATCTCGC ATCTGAAGAA GCGGAATTGC 
ATAAATCTCT TTAGAGTCTT GAATGCGTTG CGTGGGAAAA CAACGACAGT GGTTGTGCCC 
ATTGCAGATG ATGTGGCAGA CGATGGAGCA ACAGGATCTA TTCCAGTTGC CGATGCCGGA 
GCAGGAGTGG TGGCCATGAA TAGTCCCACA GTGACAGCCA GCGGAGAAGT TGTTACACCC 
GGCGGCGGCT CCGAGCGCCC AGAGAAACTG CGCGCCAAGG AACTTCTGGT CAACAAACTG 
TACGAGTGCA AGCTCTGTCC CAAGGGATTC CGCACTAAGC ACGAGTTCCG AACGCATGTC 
TACGACAAGC ACGCAGATGT CCAACGCAAG GATAACAACT CGATACAGTG CAGCTTTTGC 
GGACTGGACT TTGCCGATCC CGTTGACAGA CGGCGTCACT ACAACAACAT GGACTGCATT 
GTCCGGCTGC GCTGCATGAC GTGCGATGCC AAGCTGGAAA CGCACCAGCG ATTCCTCGAT 
CATGTCTACC AGGATCACTT GGGCGGTGTG GGTGGTGGCG CGGTCAGCGA CAACGCCTCC 
ACCACTGGCA GCGGCATGGC CAGGAGCAAC AGCATGGAAC ACTCGCCGGG CAAAAGGAGC 
CTGCTCGGCG CCTTAGGCGT TGGTTCCTCG GCGGAGGAGT CGCGAAGCAG CAGTGCGGCT 
CCGCCGCTGA CCTCTACACC AAAACTGGCG GGCGGTAATC AGGTGGGTGG CGGTGGATCG 
ACCAGCGCTT CTGCCGCCGC AGCCGCTCAG AGCTCGGCGA ATCGGGATGC ATCCGCACCC 
AAATCCCAGT ACTTCTCCCG TATGCCGCAG GTTTGTCCCA TTTGCGGCCA GCAGTACAAC 
AACTACAACA ATGTGCTGCG CCACATGGAA TCGAAGCATC CGAACAAACT GCCAGAGACA 
TACAAGTGCG TGCGCTGCGG ACTGGGCTAT CCCCGGATCT CCTACCTACG GGAGCACATG 
ATCAATGTGC ACGGAGTGGA CAAGAACCGT CACTCTGGCG GCTTCGAATA CATCGTGAAT 
GCGGATGCCG TGAAGTTGGC GGACGGAAGC ACGCCGAACG TCTACACGGG TCGCTACGAT 
TACGTGATGA AGGACCTGAT GTCGATAACA AATGATGATG AAGAGGAGGA GCCTGGCAGC 
GTGGCCAAGA AGATGCGCCT GGATGACAGT AGCAACAACA GCAGCCTGGT GGGCGTGGCT 
AGCCAGCAGA AGGAGTGCCC CATCTGCAAT GCGGTGTTCA GCAACAACAT TGGCCTGTCC 
AATCATATGC GTTCCCACTA TACGGCCTCG AATGCAGTGA ATGCAGCCTT GGCGGCTGCC 
AATCGAATGA CACCCAAATC GCTAACGATA ACCGCAACGC CAGCAACGGA TTCGGAGTTG 
GGAGTTGGAG GAACAATGTC CGAGTCGGCT CCAGCAACGC CTGCCAATGT GCCACCGGCA 
ATGGCTAACC AAACGCCCCA GGAGCAGGCA GTTTTTCGCC GGAGCCTTGA CCAGGCAGCC 
GATCGCCGCT TCCGGCGAAT GCGATGCCGC ATCTGCCAGC GTCGCTTCAG TTCGAAGAAG 
TCCTATCGCT ACCACATGCT CACCGATCAT CAGGTGCAGA ATGTGCAGTT CATCAAATGC 
AAGCTGTGCA ACGCAGAGTT TGCCTACGAG AAGGGCCTGA AGGTGCATCT GTTTAAGGTG 
CACGGAAAGG CCATCAAGGA TGAAATGATT ATCAAGCAGT TCGAGTGTGA CGTTTGCTCG 
ATTGTCTATA GTTCAGAGTC GGAGCTGCAG CAGCACAAAC GCAGCGTTCA CAAGCTGACA 
TCCGCCTCCG CTTCCACTTC GGCGTCCACG TCCTCCAAGA TTGACGACGA CTCTCTAATG 
GATGATGGCA AGCCGACATC ATCGGATCTA GCTGATCTCT CTACCCTCGC AGCGGGTGGA 
TCAACTGCAT CTGCTCCACT GTACTGGTAC CAGTGCAAGT ACTGTCCATC AAACTTTAAC 
ACCAACAAGA AGCTGGCCAT CCACATCAAC TCGCATGACG AGTTTGACTC GAACGATTAT 
TCCTGCAAAG ATTGCGGAAA TGTCTACAGC GGACGCAAGA GTCTATGGGT TCATCGCTAT 
AAGAAGCATC CGCAAGTGCC CAACCCAGCT GAGTGCTCGC TGTGCCGCAA GGTCTTTTTC 
GACCGCCAGA TGCACGACAA CCACACACCC ACCTGCAACC GCAAGCCGAT CACCTCGACA 
GGTGCCCACC AGCAGCAGGA TGGACAACTG CATTCGCACC ACACGGCCAA AAGAACAATT 
TTCCGGCATA AGACCGGCGA TGACGATGAC GAGGAGGATG ACGATGAGCA GCAGCAACTG 
GAGGAGAGGG CAAATAGCGA TGGCAATGGC ACCACTGTGG GAGTGGCGTC GGGCAGTACT 
GCAGCTGCGG GCACGTCACT AAAGATCCGC ATTCCGGAGG TGGCGTGCAC TATTTGCGGT 
GCTCGCTTCA CCGACCAGGA GCACTTTAGC AAGCACATCC AGAAGCACGA GCAAGAGCTG 
TACGTGGACA ATCCGCTGGC GGCGATGTTC GATGATGGGC CGGCGGATGC CGGTCAGTTC 
CAGGTGGAGC GGCAGAACGA GAACGGGGAA TACGCGTGCG ATTTGTGCGC CAAGACGTTC 
CCCCAGGTGA TCGCACTCAA GGTGCATCGC AAGTGGCATT TCAGAGGTGA TAGCAAGCAG 
AACCCCATCG ACGGCGAAGC GACACAGTTG ACCAACAACA ATCACACAAC CAACAACAAC 
AACAACAACT CGATGCACCT CCGCGAGCTG CATGCGGTGG GCCTGATGCC CAACCAGCAG 
CAGCAGAGCC TCAACAACTC GTGCAACAGC AGCATGAACC ACAACAACAA CAGCAGCAGC 
AACCGCAGCA AGTCGATGAA GCGGAAACGT GAGCTGAAAT GCGAATACTG CGCCTCCACC 
TTCATCAGCA ATAACAATCT GCGTCGCCAC ATGTACGAGC TGCACAAACA CGAGGTAAGC 
AATCTGCCCG AGCCTCCGGT GATTGTGGTG GACGATCACC TCACCTGCCG TCGCTGTCAG 
TTGAAGTTCG ACACAAAGGA GCTGTGGATC GAGCACAAGC TGGCCGATGC AAAGGTGGTG 
CGACCTTTCT GCCCCTTCCA GTGGGGCTGT GATCTGTGCG GCGAGTATTT GTCGCGCAAG 
GAGAAGCTAA TGAATCACAT TAACAACCAT TTGAAGGAGG AGGTCATAGT GCCAGTAGCC 
ACTAAGGCGG CCATAGAGAG AACAGCGGCC ATGGAATCAG CGGCAGCAGA TGCGAATGCA 
GCGGCGACAC TATCAGCATT GGGCGAAGGA GCCGAAACTG AAGATCAGTT TGCAGAGAAG 
GTTGAGGCTG CAGGGGCAAC AACAACAGAT AAGTTGACGA ATCCCGACGA GGAGGATAGC 
GATGATTTGG ATGAGGATAG CTCGGGCGAC GACGATGATA GTTCGGGGAC GGGAGATGAT 
GACGATGACG ACGACAGCGA CGATGATGAG GATGGCGAGG GTGAAGATGA GGACGAGGAG 
GGAGATGGTG GCGAAGGCGA GGACGAAGAG GGTGTCCAGC CACCCGCTCA ACTTTTGCCG 
CAGCAGCAAC ACAAAACGGA TCTTAATCTT AACCAGGACG ACGATGATCT CGTCGAGGAG 
GTCATCAGCT CCGATGACGA CGAGGACGAT GATGGAGAGG TGGAAAGCGA CGATGACGAC 
GAAGATGATG ATGATGAGGA GGACGATGTG GAGGAGCCGG AGCCAGTTGG ATTGACGGTG 
CGACCGCTAA TGAATGGCAA ATCAAAGATG CCGCCGCTAA TAGTGGCCAG TTCGGATGAT 
GAAGACGATG GTGTGATGCC CATAGAGGAC ATTATCGAAG AGGAGTTCGA TGAGGATGCC 
GATCCAGATC CGGAGGATGC CATTGAGGAG GTAGACGAAG ATGATTTAGA TGAGGGGGAA 
GTGGAGGACG AGCCGAATGT GGTGTCGACG GCCTCCTTCT CGGAGAGCGA GTCCAGCACA 
ACGACCACGT CCAATTCGCA TTCGCATTCG CATTCAACGG GAGCTTCTCC AGAGCCACAT 
CAAAAGCCAT ATCCTCAATG GGCCAAAGCT CTCGACAGTG TCAGCAGCAG CAGCAGCAGC 
AGCAGCCGCC GCCACAGCAA GCAGCAAGGC AACAGCATTA CTAACGGCAG CAAAGGCGAA 
GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hang ( NM_167520.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727979.3 Drosophila melanogaster hangover (hang), transcript variant D, mRNA.

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CloneID	OFa21500
Clone ID Related Accession (Same CDS sequence)	NM_167520.3
Accession Version	NM_167520.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5880bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	hangover, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167520.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGCGACG CTGCGGCGGC AACAGCGACA ACAACAACAA CAGCAGCAGT AGCAGCAGCC 
GTCGCAACAA CAACAGCATC GGTAGCATTG GAGGCCACAG CGACACAGCC TGGAACGACA 
ACGACGACGG TAGCCACTGC GTCAGCGGGA ACCACATCCC CTGAAGCGGC CATTCCAACA 
GCAGCAACAG CAACATCGGC GAGAAACAGC AACAGCGAAC GGTCGGCGCG ACAAAACTGC 
TGTCGCCTGT GCATCGCCCC ACAAACCGAA TGCATCTCGA TCATCAATAG CTATGCGGCC 
GACAAGGAGC CGCTGTCCAC CAAGATCCAC AACTGCGTCG GCATCAAGGT TACACCGCAG 
GATCGGCTGT CGCAGCAAAT TTGCCACGCC TGCATCAGTT ACCTGAACTC ATGGCAGAGC 
TTCAAGAACC GGTGCTTCAG CTCGCAAGCG AAGCAGCGCC AGTGGCTGGA TACCAACAAG 
AGCAAGCTCC TCAACTACCT GGACCTGAAC AGTGCCGAGA ATGGGGGAGG AGGCTTCTTC 
GATCAGCATC TGCATCAACA ACAGCAACAC CACCAGCACT TGGAGAATGA GCTCGAAGCG 
GAGAAGGAGA AGGCGACGCC AACGGCCGCA TCGACAGCCG CCAACATATT GGACGGCATA 
CACTCGCTGA AGAAACGCAA ATCCCTAACA GTCTATCCAC TGCCTGCGAT GCCCATCAAA 
GATGAGCCGA TTGATACGGA TGACGACTAT CAGATGAAGT CCATAGACGA GTCCGATGAC 
ATGGTCGATC CCACAATGTT CCTAGAGCGC TCCGAGCACG AGGGTGATGT CCCGCTGACG 
GCCTCGGATT ACGACTACAC TGCGCAGCAT GGCGTGAATG CTTCGTCCGT GGCTGCCTCG 
CTGCCGCCGA ATGCGGTGGC CAATGTGGCA GCCGCCGGGG ACTCCAAGGT GGCCAGCTGC 
AGGGCCTGCA GCCTGCAGTT CTCTACCCGG GCCAACGCCC GTCGCCACGA AAGGAATCTA 
CACCCAAACC TGTTCCAGTT GTCCACAGAC TCCCCCCACA ACACACCCAT CACAAAGCCA 
ACGCCCGCTT TGGCTGCCGC CTTGGAAATG CAACGGGCAG CGGCTGCGGC GGCCACCGCT 
GAGGCAAATC GGGCAGCGGG CGCCGCTGGC GGGAATATCT CCACGCAAAA GTACCGCCAG 
GTGGTGATGA ACGCGTTCAT TAAGTGCGAG GGCGGCGGCT ACGACTACGA TAATCCCGAA 
CAGTACCGAC CATTGCTCAC CCGCGACAAA GTGGAGTTCA TTGAGCAGAA CGATGAGTTC 
CTCGAGCAAT ACCAGACGAT GACCTGCCGA TGTTGCAACA AATACTTCAG CACGTACAAG 
AACTTCATGG CGCACGTTCG CAAGAAGTAT CCGCAGCTGC CGCGCAACCT ATGCTTCAAT 
TGCCTCAAGA TGAACGACTC CAAGGCGCTG TTCATCTCGC ATCTGAAGAA GCGGAATTGC 
ATAAATCTCT TTAGAGTCTT GAATGCGTTG CGTGGGAAAA CAACGACAGT GGTTGTGCCC 
ATTGCAGATG ATGTGGCAGA CGATGGAGCA ACAGGATCTA TTCCAGTTGC CGATGCCGGA 
GCAGGAGTGG TGGCCATGAA TAGTCCCACA GTGACAGCCA GCGGAGAAGT TGTTACACCC 
GGCGGCGGCT CCGAGCGCCC AGAGAAACTG CGCGCCAAGG AACTTCTGGT CAACAAACTG 
TACGAGTGCA AGCTCTGTCC CAAGGGATTC CGCACTAAGC ACGAGTTCCG AACGCATGTC 
TACGACAAGC ACGCAGATGT CCAACGCAAG GATAACAACT CGATACAGTG CAGCTTTTGC 
GGACTGGACT TTGCCGATCC CGTTGACAGA CGGCGTCACT ACAACAACAT GGACTGCATT 
GTCCGGCTGC GCTGCATGAC GTGCGATGCC AAGCTGGAAA CGCACCAGCG ATTCCTCGAT 
CATGTCTACC AGGATCACTT GGGCGGTGTG GGTGGTGGCG CGGTCAGCGA CAACGCCTCC 
ACCACTGGCA GCGGCATGGC CAGGAGCAAC AGCATGGAAC ACTCGCCGGG CAAAAGGAGC 
CTGCTCGGCG CCTTAGGCGT TGGTTCCTCG GCGGAGGAGT CGCGAAGCAG CAGTGCGGCT 
CCGCCGCTGA CCTCTACACC AAAACTGGCG GGCGGTAATC AGGTGGGTGG CGGTGGATCG 
ACCAGCGCTT CTGCCGCCGC AGCCGCTCAG AGCTCGGCGA ATCGGGATGC ATCCGCACCC 
AAATCCCAGT ACTTCTCCCG TATGCCGCAG GTTTGTCCCA TTTGCGGCCA GCAGTACAAC 
AACTACAACA ATGTGCTGCG CCACATGGAA TCGAAGCATC CGAACAAACT GCCAGAGACA 
TACAAGTGCG TGCGCTGCGG ACTGGGCTAT CCCCGGATCT CCTACCTACG GGAGCACATG 
ATCAATGTGC ACGGAGTGGA CAAGAACCGT CACTCTGGCG GCTTCGAATA CATCGTGAAT 
GCGGATGCCG TGAAGTTGGC GGACGGAAGC ACGCCGAACG TCTACACGGG TCGCTACGAT 
TACGTGATGA AGGACCTGAT GTCGATAACA AATGGTGGGA CACTCGATGA TGAAGAGGAG 
GAGCCTGGCA GCGTGGCCAA GAAGATGCGC CTGGATGACA GTAGCAACAA CAGCAGCCTG 
GTGGGCGTGG CTAGCCAGCA GAAGGAGTGC CCCATCTGCA ATGCGGTGTT CAGCAACAAC 
ATTGGCCTGT CCAATCATAT GCGTTCCCAC TATACGGCCT CGAATGCAGT GAATGCAGCC 
TTGGCGGCTG CCAATCGAAT GACACCCAAA TCGCTAACGA TAACCGCAAC GCCAGCAACG 
GATTCGGAGT TGGGAGTTGG AGGAACAATG TCCGAGTCGG CTCCAGCAAC GCCTGCCAAT 
GTGCCACCGG CAATGGCTAA CCAAACGCCC CAGGAGCAGG CAGTTTTTCG CCGGAGCCTT 
GACCAGGCAG CCGATCGCCG CTTCCGGCGA ATGCGATGCC GCATCTGCCA GCGTCGCTTC 
AGTTCGAAGA AGTCCTATCG CTACCACATG CTCACCGATC ATCAGGTGCA GAATGTGCAG 
TTCATCAAAT GCAAGCTGTG CAACGCAGAG TTTGCCTACG AGAAGGGCCT GAAGGTGCAT 
CTGTTTAAGG TGCACGGAAA GGCCATCAAG GATGAAATGA TTATCAAGCA GTTCGAGTGT 
GACGTTTGCT CGATTGTCTA TAGTTCAGAG TCGGAGCTGC AGCAGCACAA ACGCAGCGTT 
CACAAGCTGA CATCCGCCTC CGCTTCCACT TCGGCGTCCA CGTCCTCCAA GATTGACGAC 
GACTCTCTAA TGGATGATGG CAAGCCGACA TCATCGGATC TAGCTGATCT CTCTACCCTC 
GCAGCGGGTG GATCAACTGC ATCTGCTCCA CTGTACTGGT ACCAGTGCAA GTACTGTCCA 
TCAAACTTTA ACACCAACAA GAAGCTGGCC ATCCACATCA ACTCGCATGA CGAGTTTGAC 
TCGAACGATT ATTCCTGCAA AGATTGCGGA AATGTCTACA GCGGACGCAA GAGTCTATGG 
GTTCATCGCT ATAAGAAGCA TCCGCAAGTG CCCAACCCAG CTGAGTGCTC GCTGTGCCGC 
AAGGTCTTTT TCGACCGCCA GATGCACGAC AACCACACAC CCACCTGCAA CCGCAAGCCG 
ATCACCTCGA CAGGTGCCCA CCAGCAGCAG GATGGACAAC TGCATTCGCA CCACACGGCC 
AAAAGAACAA TTTTCCGGCA TAAGACCGGC GATGACGATG ACGAGGAGGA TGACGATGAG 
CAGCAGCAAC TGGAGGAGAG GGCAAATAGC GATGGCAATG GCACCACTGT GGGAGTGGCG 
TCGGGCAGTA CTGCAGCTGC GGGCACGTCA CTAAAGATCC GCATTCCGGA GGTGGCGTGC 
ACTATTTGCG GTGCTCGCTT CACCGACCAG GAGCACTTTA GCAAGCACAT CCAGAAGCAC 
GAGCAAGAGC TGTACGTGGA CAATCCGCTG GCGGCGATGT TCGATGATGG GCCGGCGGAT 
GCCGGTCAGT TCCAGGTGGA GCGGCAGAAC GAGAACGGGG AATACGCGTG CGATTTGTGC 
GCCAAGACGT TCCCCCAGGT GATCGCACTC AAGGTGCATC GCAAGTGGCA TTTCAGAGGT 
GATAGCAAGC AGAACCCCAT CGACGGCGAA GCGACACAGT TGACCAACAA CAATCACACA 
ACCAACAACA ACAACAACAA CTCGATGCAC CTCCGCGAGC TGCATGCGGT GGGCCTGATG 
CCCAACCAGC AGCAGCAGAG CCTCAACAAC TCGTGCAACA GCAGCATGAA CCACAACAAC 
AACAGCAGCA GCAACCGCAG CAAGTCGATG AAGCGGAAAC GTGAGCTGAA ATGCGAATAC 
TGCGCCTCCA CCTTCATCAG CAATAACAAT CTGCGTCGCC ACATGTACGA GCTGCACAAA 
CACGAGGTAA GCAATCTGCC CGAGCCTCCG GTGATTGTGG TGGACGATCA CCTCACCTGC 
CGTCGCTGTC AGTTGAAGTT CGACACAAAG GAGCTGTGGA TCGAGCACAA GCTGGCCGAT 
GCAAAGGTGG TGCGACCTTT CTGCCCCTTC CAGTGGGGCT GTGATCTGTG CGGCGAGTAT 
TTGTCGCGCA AGGAGAAGCT AATGAATCAC ATTAACAACC ATTTGAAGGA GGAGGTCATA 
GTGCCAGTAG CCACTAAGGC GGCCATAGAG AGAACAGCGG CCATGGAATC AGCGGCAGCA 
GATGCGAATG CAGCGGCGAC ACTATCAGCA TTGGGCGAAG GAGCCGAAAC TGAAGATCAG 
TTTGCAGAGA AGGTTGAGGC TGCAGGGGCA ACAACAACAG ATAAGTTGAC GAATCCCGAC 
GAGGAGGATA GCGATGATTT GGATGAGGAT AGCTCGGGCG ACGACGATGA TAGTTCGGGG 
ACGGGAGATG ATGACGATGA CGACGACAGC GACGATGATG AGGATGGCGA GGGTGAAGAT 
GAGGACGAGG AGGGAGATGG TGGCGAAGGC GAGGACGAAG AGGGTGTCCA GCCACCCGCT 
CAACTTTTGC CGCAGCAGCA ACACAAAACG GATCTTAATC TTAACCAGGA CGACGATGAT 
CTCGTCGAGG AGGTCATCAG CTCCGATGAC GACGAGGACG ATGATGGAGA GGTGGAAAGC 
GACGATGACG ACGAAGATGA TGATGATGAG GAGGACGATG TGGAGGAGCC GGAGCCAGTT 
GGATTGACGG TGCGACCGCT AATGAATGGC AAATCAAAGA TGCCGCCGCT AATAGTGGCC 
AGTTCGGATG ATGAAGACGA TGGTGTGATG CCCATAGAGG ACATTATCGA AGAGGAGTTC 
GATGAGGATG CCGATCCAGA TCCGGAGGAT GCCATTGAGG AGGTAGACGA AGATGATTTA 
GATGAGGGGG AAGTGGAGGA CGAGCCGAAT GTGGTGTCGA CGGCCTCCTT CTCGGAGAGC 
GAGTCCAGCA CAACGACCAC GTCCAATTCG CATTCGCATT CGCATTCAAC GGGTGAGCGG 
CGTAAGAAGG CCGTCGATCA GTTGAATGAT CCCGGCTTTA CCTGTGATCT ATGTCAACTT 
TGTTTCGATT CTCAGGAGCT TCTCCAGAGC CACATCAAAA GCCATATCCT CAATGGGCCA 
AAGCTCTCGA CAGTGTCAGC AGCAGCAGCA GCAGCAGCAG CCGCCGCCAC AGCAAGCAGC 
AAGGCAACAG CATTACTAAC GGCAGCAAAG GCGAAGCCTG ACTCCAAGTC AGCGGTGCTG 
GCCAACAATA ATAACAGCAA GACAAGCAGT AAGACTGTGG CAGCAGGTGC GACAAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727979.3
CDS	450..6329
Translation	MCDAAAATATTTTTAAVAAAVATTTASVALEATATQPGTTTTTVATASAGTTSPEAAIPTAATATSARNSNSERSARQNCCRLCIAPQTECISIINSYAADKEPLSTKIHNCVGIKVTPQDRLSQQICHACISYLNSWQSFKNRCFSSQAKQRQWLDTNKSKLLNYLDLNSAENGGGGFFDQHLHQQQQHHQHLENELEAEKEKATPTAASTAANILDGIHSLKKRKSLTVYPLPAMPIKDEPIDTDDDYQMKSIDESDDMVDPTMFLERSEHEGDVPLTASDYDYTAQHGVNASSVAASLPPNAVANVAAAGDSKVASCRACSLQFSTRANARRHERNLHPNLFQLSTDSPHNTPITKPTPALAAALEMQRAAAAAATAEANRAAGAAGGNISTQKYRQVVMNAFIKCEGGGYDYDNPEQYRPLLTRDKVEFIEQNDEFLEQYQTMTCRCCNKYFSTYKNFMAHVRKKYPQLPRNLCFNCLKMNDSKALFISHLKKRNCINLFRVLNALRGKTTTVVVPIADDVADDGATGSIPVADAGAGVVAMNSPTVTASGEVVTPGGGSERPEKLRAKELLVNKLYECKLCPKGFRTKHEFRTHVYDKHADVQRKDNNSIQCSFCGLDFADPVDRRRHYNNMDCIVRLRCMTCDAKLETHQRFLDHVYQDHLGGVGGGAVSDNASTTGSGMARSNSMEHSPGKRSLLGALGVGSSAEESRSSSAAPPLTSTPKLAGGNQVGGGGSTSASAAAAAQSSANRDASAPKSQYFSRMPQVCPICGQQYNNYNNVLRHMESKHPNKLPETYKCVRCGLGYPRISYLREHMINVHGVDKNRHSGGFEYIVNADAVKLADGSTPNVYTGRYDYVMKDLMSITNGGTLDDEEEEPGSVAKKMRLDDSSNNSSLVGVASQQKECPICNAVFSNNIGLSNHMRSHYTASNAVNAALAAANRMTPKSLTITATPATDSELGVGGTMSESAPATPANVPPAMANQTPQEQAVFRRSLDQAADRRFRRMRCRICQRRFSSKKSYRYHMLTDHQVQNVQFIKCKLCNAEFAYEKGLKVHLFKVHGKAIKDEMIIKQFECDVCSIVYSSESELQQHKRSVHKLTSASASTSASTSSKIDDDSLMDDGKPTSSDLADLSTLAAGGSTASAPLYWYQCKYCPSNFNTNKKLAIHINSHDEFDSNDYSCKDCGNVYSGRKSLWVHRYKKHPQVPNPAECSLCRKVFFDRQMHDNHTPTCNRKPITSTGAHQQQDGQLHSHHTAKRTIFRHKTGDDDDEEDDDEQQQLEERANSDGNGTTVGVASGSTAAAGTSLKIRIPEVACTICGARFTDQEHFSKHIQKHEQELYVDNPLAAMFDDGPADAGQFQVERQNENGEYACDLCAKTFPQVIALKVHRKWHFRGDSKQNPIDGEATQLTNNNHTTNNNNNNSMHLRELHAVGLMPNQQQQSLNNSCNSSMNHNNNSSSNRSKSMKRKRELKCEYCASTFISNNNLRRHMYELHKHEVSNLPEPPVIVVDDHLTCRRCQLKFDTKELWIEHKLADAKVVRPFCPFQWGCDLCGEYLSRKEKLMNHINNHLKEEVIVPVATKAAIERTAAMESAAADANAAATLSALGEGAETEDQFAEKVEAAGATTTDKLTNPDEEDSDDLDEDSSGDDDDSSGTGDDDDDDDSDDDEDGEGEDEDEEGDGGEGEDEEGVQPPAQLLPQQQHKTDLNLNQDDDDLVEEVISSDDDEDDDGEVESDDDDEDDDDEEDDVEEPEPVGLTVRPLMNGKSKMPPLIVASSDDEDDGVMPIEDIIEEEFDEDADPDPEDAIEEVDEDDLDEGEVEDEPNVVSTASFSESESSTTTTSNSHSHSHSTGERRKKAVDQLNDPGFTCDLCQLCFDSQELLQSHIKSHILNGPKLSTVSAAAAAAAAAATASSKATALLTAAKAKPDSKSAVLANNNNSKTSSKTVAAGATN

Target ORF information:

RefSeq Version	NM_167520.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster hangover (hang), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167520.3

ORF Insert Sequence:

ATGTGCGACG CTGCGGCGGC AACAGCGACA ACAACAACAA CAGCAGCAGT AGCAGCAGCC 
GTCGCAACAA CAACAGCATC GGTAGCATTG GAGGCCACAG CGACACAGCC TGGAACGACA 
ACGACGACGG TAGCCACTGC GTCAGCGGGA ACCACATCCC CTGAAGCGGC CATTCCAACA 
GCAGCAACAG CAACATCGGC GAGAAACAGC AACAGCGAAC GGTCGGCGCG ACAAAACTGC 
TGTCGCCTGT GCATCGCCCC ACAAACCGAA TGCATCTCGA TCATCAATAG CTATGCGGCC 
GACAAGGAGC CGCTGTCCAC CAAGATCCAC AACTGCGTCG GCATCAAGGT TACACCGCAG 
GATCGGCTGT CGCAGCAAAT TTGCCACGCC TGCATCAGTT ACCTGAACTC ATGGCAGAGC 
TTCAAGAACC GGTGCTTCAG CTCGCAAGCG AAGCAGCGCC AGTGGCTGGA TACCAACAAG 
AGCAAGCTCC TCAACTACCT GGACCTGAAC AGTGCCGAGA ATGGGGGAGG AGGCTTCTTC 
GATCAGCATC TGCATCAACA ACAGCAACAC CACCAGCACT TGGAGAATGA GCTCGAAGCG 
GAGAAGGAGA AGGCGACGCC AACGGCCGCA TCGACAGCCG CCAACATATT GGACGGCATA 
CACTCGCTGA AGAAACGCAA ATCCCTAACA GTCTATCCAC TGCCTGCGAT GCCCATCAAA 
GATGAGCCGA TTGATACGGA TGACGACTAT CAGATGAAGT CCATAGACGA GTCCGATGAC 
ATGGTCGATC CCACAATGTT CCTAGAGCGC TCCGAGCACG AGGGTGATGT CCCGCTGACG 
GCCTCGGATT ACGACTACAC TGCGCAGCAT GGCGTGAATG CTTCGTCCGT GGCTGCCTCG 
CTGCCGCCGA ATGCGGTGGC CAATGTGGCA GCCGCCGGGG ACTCCAAGGT GGCCAGCTGC 
AGGGCCTGCA GCCTGCAGTT CTCTACCCGG GCCAACGCCC GTCGCCACGA AAGGAATCTA 
CACCCAAACC TGTTCCAGTT GTCCACAGAC TCCCCCCACA ACACACCCAT CACAAAGCCA 
ACGCCCGCTT TGGCTGCCGC CTTGGAAATG CAACGGGCAG CGGCTGCGGC GGCCACCGCT 
GAGGCAAATC GGGCAGCGGG CGCCGCTGGC GGGAATATCT CCACGCAAAA GTACCGCCAG 
GTGGTGATGA ACGCGTTCAT TAAGTGCGAG GGCGGCGGCT ACGACTACGA TAATCCCGAA 
CAGTACCGAC CATTGCTCAC CCGCGACAAA GTGGAGTTCA TTGAGCAGAA CGATGAGTTC 
CTCGAGCAAT ACCAGACGAT GACCTGCCGA TGTTGCAACA AATACTTCAG CACGTACAAG 
AACTTCATGG CGCACGTTCG CAAGAAGTAT CCGCAGCTGC CGCGCAACCT ATGCTTCAAT 
TGCCTCAAGA TGAACGACTC CAAGGCGCTG TTCATCTCGC ATCTGAAGAA GCGGAATTGC 
ATAAATCTCT TTAGAGTCTT GAATGCGTTG CGTGGGAAAA CAACGACAGT GGTTGTGCCC 
ATTGCAGATG ATGTGGCAGA CGATGGAGCA ACAGGATCTA TTCCAGTTGC CGATGCCGGA 
GCAGGAGTGG TGGCCATGAA TAGTCCCACA GTGACAGCCA GCGGAGAAGT TGTTACACCC 
GGCGGCGGCT CCGAGCGCCC AGAGAAACTG CGCGCCAAGG AACTTCTGGT CAACAAACTG 
TACGAGTGCA AGCTCTGTCC CAAGGGATTC CGCACTAAGC ACGAGTTCCG AACGCATGTC 
TACGACAAGC ACGCAGATGT CCAACGCAAG GATAACAACT CGATACAGTG CAGCTTTTGC 
GGACTGGACT TTGCCGATCC CGTTGACAGA CGGCGTCACT ACAACAACAT GGACTGCATT 
GTCCGGCTGC GCTGCATGAC GTGCGATGCC AAGCTGGAAA CGCACCAGCG ATTCCTCGAT 
CATGTCTACC AGGATCACTT GGGCGGTGTG GGTGGTGGCG CGGTCAGCGA CAACGCCTCC 
ACCACTGGCA GCGGCATGGC CAGGAGCAAC AGCATGGAAC ACTCGCCGGG CAAAAGGAGC 
CTGCTCGGCG CCTTAGGCGT TGGTTCCTCG GCGGAGGAGT CGCGAAGCAG CAGTGCGGCT 
CCGCCGCTGA CCTCTACACC AAAACTGGCG GGCGGTAATC AGGTGGGTGG CGGTGGATCG 
ACCAGCGCTT CTGCCGCCGC AGCCGCTCAG AGCTCGGCGA ATCGGGATGC ATCCGCACCC 
AAATCCCAGT ACTTCTCCCG TATGCCGCAG GTTTGTCCCA TTTGCGGCCA GCAGTACAAC 
AACTACAACA ATGTGCTGCG CCACATGGAA TCGAAGCATC CGAACAAACT GCCAGAGACA 
TACAAGTGCG TGCGCTGCGG ACTGGGCTAT CCCCGGATCT CCTACCTACG GGAGCACATG 
ATCAATGTGC ACGGAGTGGA CAAGAACCGT CACTCTGGCG GCTTCGAATA CATCGTGAAT 
GCGGATGCCG TGAAGTTGGC GGACGGAAGC ACGCCGAACG TCTACACGGG TCGCTACGAT 
TACGTGATGA AGGACCTGAT GTCGATAACA AATGGTGGGA CACTCGATGA TGAAGAGGAG 
GAGCCTGGCA GCGTGGCCAA GAAGATGCGC CTGGATGACA GTAGCAACAA CAGCAGCCTG 
GTGGGCGTGG CTAGCCAGCA GAAGGAGTGC CCCATCTGCA ATGCGGTGTT CAGCAACAAC 
ATTGGCCTGT CCAATCATAT GCGTTCCCAC TATACGGCCT CGAATGCAGT GAATGCAGCC 
TTGGCGGCTG CCAATCGAAT GACACCCAAA TCGCTAACGA TAACCGCAAC GCCAGCAACG 
GATTCGGAGT TGGGAGTTGG AGGAACAATG TCCGAGTCGG CTCCAGCAAC GCCTGCCAAT 
GTGCCACCGG CAATGGCTAA CCAAACGCCC CAGGAGCAGG CAGTTTTTCG CCGGAGCCTT 
GACCAGGCAG CCGATCGCCG CTTCCGGCGA ATGCGATGCC GCATCTGCCA GCGTCGCTTC 
AGTTCGAAGA AGTCCTATCG CTACCACATG CTCACCGATC ATCAGGTGCA GAATGTGCAG 
TTCATCAAAT GCAAGCTGTG CAACGCAGAG TTTGCCTACG AGAAGGGCCT GAAGGTGCAT 
CTGTTTAAGG TGCACGGAAA GGCCATCAAG GATGAAATGA TTATCAAGCA GTTCGAGTGT 
GACGTTTGCT CGATTGTCTA TAGTTCAGAG TCGGAGCTGC AGCAGCACAA ACGCAGCGTT 
CACAAGCTGA CATCCGCCTC CGCTTCCACT TCGGCGTCCA CGTCCTCCAA GATTGACGAC 
GACTCTCTAA TGGATGATGG CAAGCCGACA TCATCGGATC TAGCTGATCT CTCTACCCTC 
GCAGCGGGTG GATCAACTGC ATCTGCTCCA CTGTACTGGT ACCAGTGCAA GTACTGTCCA 
TCAAACTTTA ACACCAACAA GAAGCTGGCC ATCCACATCA ACTCGCATGA CGAGTTTGAC 
TCGAACGATT ATTCCTGCAA AGATTGCGGA AATGTCTACA GCGGACGCAA GAGTCTATGG 
GTTCATCGCT ATAAGAAGCA TCCGCAAGTG CCCAACCCAG CTGAGTGCTC GCTGTGCCGC 
AAGGTCTTTT TCGACCGCCA GATGCACGAC AACCACACAC CCACCTGCAA CCGCAAGCCG 
ATCACCTCGA CAGGTGCCCA CCAGCAGCAG GATGGACAAC TGCATTCGCA CCACACGGCC 
AAAAGAACAA TTTTCCGGCA TAAGACCGGC GATGACGATG ACGAGGAGGA TGACGATGAG 
CAGCAGCAAC TGGAGGAGAG GGCAAATAGC GATGGCAATG GCACCACTGT GGGAGTGGCG 
TCGGGCAGTA CTGCAGCTGC GGGCACGTCA CTAAAGATCC GCATTCCGGA GGTGGCGTGC 
ACTATTTGCG GTGCTCGCTT CACCGACCAG GAGCACTTTA GCAAGCACAT CCAGAAGCAC 
GAGCAAGAGC TGTACGTGGA CAATCCGCTG GCGGCGATGT TCGATGATGG GCCGGCGGAT 
GCCGGTCAGT TCCAGGTGGA GCGGCAGAAC GAGAACGGGG AATACGCGTG CGATTTGTGC 
GCCAAGACGT TCCCCCAGGT GATCGCACTC AAGGTGCATC GCAAGTGGCA TTTCAGAGGT 
GATAGCAAGC AGAACCCCAT CGACGGCGAA GCGACACAGT TGACCAACAA CAATCACACA 
ACCAACAACA ACAACAACAA CTCGATGCAC CTCCGCGAGC TGCATGCGGT GGGCCTGATG 
CCCAACCAGC AGCAGCAGAG CCTCAACAAC TCGTGCAACA GCAGCATGAA CCACAACAAC 
AACAGCAGCA GCAACCGCAG CAAGTCGATG AAGCGGAAAC GTGAGCTGAA ATGCGAATAC 
TGCGCCTCCA CCTTCATCAG CAATAACAAT CTGCGTCGCC ACATGTACGA GCTGCACAAA 
CACGAGGTAA GCAATCTGCC CGAGCCTCCG GTGATTGTGG TGGACGATCA CCTCACCTGC 
CGTCGCTGTC AGTTGAAGTT CGACACAAAG GAGCTGTGGA TCGAGCACAA GCTGGCCGAT 
GCAAAGGTGG TGCGACCTTT CTGCCCCTTC CAGTGGGGCT GTGATCTGTG CGGCGAGTAT 
TTGTCGCGCA AGGAGAAGCT AATGAATCAC ATTAACAACC ATTTGAAGGA GGAGGTCATA 
GTGCCAGTAG CCACTAAGGC GGCCATAGAG AGAACAGCGG CCATGGAATC AGCGGCAGCA 
GATGCGAATG CAGCGGCGAC ACTATCAGCA TTGGGCGAAG GAGCCGAAAC TGAAGATCAG 
TTTGCAGAGA AGGTTGAGGC TGCAGGGGCA ACAACAACAG ATAAGTTGAC GAATCCCGAC 
GAGGAGGATA GCGATGATTT GGATGAGGAT AGCTCGGGCG ACGACGATGA TAGTTCGGGG 
ACGGGAGATG ATGACGATGA CGACGACAGC GACGATGATG AGGATGGCGA GGGTGAAGAT 
GAGGACGAGG AGGGAGATGG TGGCGAAGGC GAGGACGAAG AGGGTGTCCA GCCACCCGCT 
CAACTTTTGC CGCAGCAGCA ACACAAAACG GATCTTAATC TTAACCAGGA CGACGATGAT 
CTCGTCGAGG AGGTCATCAG CTCCGATGAC GACGAGGACG ATGATGGAGA GGTGGAAAGC 
GACGATGACG ACGAAGATGA TGATGATGAG GAGGACGATG TGGAGGAGCC GGAGCCAGTT 
GGATTGACGG TGCGACCGCT AATGAATGGC AAATCAAAGA TGCCGCCGCT AATAGTGGCC 
AGTTCGGATG ATGAAGACGA TGGTGTGATG CCCATAGAGG ACATTATCGA AGAGGAGTTC 
GATGAGGATG CCGATCCAGA TCCGGAGGAT GCCATTGAGG AGGTAGACGA AGATGATTTA 
GATGAGGGGG AAGTGGAGGA CGAGCCGAAT GTGGTGTCGA CGGCCTCCTT CTCGGAGAGC 
GAGTCCAGCA CAACGACCAC GTCCAATTCG CATTCGCATT CGCATTCAAC GGGTGAGCGG 
CGTAAGAAGG CCGTCGATCA GTTGAATGAT CCCGGCTTTA CCTGTGATCT ATGTCAACTT 
TGTTTCGATT CTCAGGAGCT TCTCCAGAGC CACATCAAAA GCCATATCCT CAATGGGCCA 
AAGCTCTCGA CAGTGTCAGC AGCAGCAGCA GCAGCAGCAG CCGCCGCCAC AGCAAGCAGC 
AAGGCAACAG CATTACTAAC GGCAGCAAAG GCGAAGCCTG ACTCCAAGTC AGCGGTGCTG 
GCCAACAATA ATAACAGCAA GACAAGCAGT AAGACTGTGG CAGCAGGTGC GACAAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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hang cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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