Mfe2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mfe2
Entrez Gene ID	32582
Full Name	peroxisomal Multifunctional enzyme type 2
Synonyms	CG3415,DmMFE-2,Dmel\CG3415,MFE-2
General protein information	Preferred Names peroxisomal Multifunctional enzyme type 2 Names CG3415 gene product from transcript CG3415-RA CG3415-PA CG3415-PB Mfe2-PA Mfe2-PB multifunctional enzyme type 2 peroxisomal multifunctional enzyme type 2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 14B7-14B7

mRNA and Protein(s)

mRNA	Protein	Name
NM_132881.3	NP_573109.1	peroxisomal multifunctional enzyme type 2, isoform A
NM_001298389.1	NP_001285318.1	peroxisomal multifunctional enzyme type 2, isoform B

Pathways from BioSystems

Homologies

Homo sapiens (human)	HSD17B4	NP_000405.1
Mus musculus (house mouse)	Hsd17b4	NP_032318.2
Eremothecium gossypii	AGOS_AGL060W	NP_986606.1
Neurospora crassa	NCU08828	XP_962039.1
Arabidopsis thaliana (thale cress)	ECH2	NP_177742.2
Rattus norvegicus (Norway rat)	Hsd17b4	NP_077368.2
Danio rerio (zebrafish)	hsd17b4	NP_956430.1
Oryza sativa (rice)	Os09g0544900	NP_001063833.1
	HSD17B4	XP_001087837.2
Canis lupus familiaris (dog)	HSD17B4	XP_531860.2
Bos taurus (cattle)	HSD17B4	NP_001007810.1
Gallus gallus (chicken)	HSD17B4	NP_990274.1
Drosophila melanogaster (fruit fly)	Mfe2	NP_573109.1
Caenorhabditis elegans (roundworm)	dhs-28	NP_509146.1
Saccharomyces cerevisiae (baker's yeast)	FOX2	NP_012934.1
Kluyveromyces lactis	KLLA0E13817g	XP_454574.1
Xenopus tropicalis (tropical clawed frog)	hsd17b4	NP_001027490.1
Pan troglodytes (chimpanzee)	HSD17B4	XP_003310890.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008667	XP_314766.4
Magnaporthe oryzae (rice blast fungus)	MGG_06148	XP_003712026.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Molecular models of domain structure of MFE-2 from human, C. elegans, and Drosophila melanogaster lend support to possible structural role of MFE-2 domains including SCP-2L (sterol carrier protein 2-like) domain in human and C. elegans proteins.
Title: Quaternary structure of human, Drosophila melanogaster and Caenorhabditis elegans MFE-2 in solution from synchrotron small-angle X-ray scattering.

The following Mfe2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mfe2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa01761	NM_001298389.1 Latest version!	Drosophila melanogaster peroxisomal multifunctional enzyme type 2, transcript variant B (Mfe2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa01761	NM_132881.3 Latest version!	Drosophila melanogaster peroxisomal multifunctional enzyme type 2, transcript variant A (Mfe2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mfe2 ( NM_132881.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_573109.1 Drosophila melanogaster peroxisomal multifunctional enzyme type 2, transcript variant A (Mfe2), mRNA.

Price & Availability↑

CloneID	OFa01761
Clone ID Related Accession (Same CDS sequence)	NM_132881.3 , NM_001298389.1
Accession Version	NM_132881.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1797bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	peroxisomal multifunctional enzyme type 2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_132881.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCTCAT CCGATGGAAA ACTTCGTTAC GATGGCCGTG TGGCGGTGGT GACGGGAGCT 
GGTGCCGGTT TGGGCCGCGA GTACGCACTG CTCTTCGCGG AGCGTGGCGC CAAGGTGGTG 
GTCAACGATC TGGGTGGCAC CCACTCCGGC GATGGAGCAT CGCAGCGGGC CGCCGACATT 
GTGGTGGATG AGATCCGTAA GGCCGGCGGC GAGGCGGTGG CCGACTACAA CTCGGTAATC 
GATGGGGCAA AGGTCATCGA GACGGCCATC AAAGCTTTCG GACGCGTCGA TATCCTGGTC 
AACAATGCCG GCATTCTACG CGACAGGAGT CTGGTGAAGA CCAGCGAACA GGACTGGAAT 
CTGGTCAACG ATGTCCATCT GAAGGGCAGC TTTAAGTGCA CCCAGGCGGC ATTCCCGTAC 
ATGAAGAAGC AGAACTATGG CCGCATCATC ATGACATCGT CCAACTCGGG CATCTATGGT 
AATTTCGGGC AGGTCAACTA CACTGCCGCC AAGATGGGCC TCATCGGATT GGCCAACACG 
GTTGCCATCG AGGGAGCCCG CAACAATGTT CTTTGCAACG TCATCGTGCC AACTGCCGCC 
AGTCGAATGA CCGAGGGCAT TCTGCCCGAC ATTCTCTTCA ACGAGCTGAA GCCCAAGCTG 
ATTGCTCCCG TAGTGGCCTA CTTGTGCCAC GAGTCCTGCG AGGATAATGG TAGCTATATC 
GAGAGTGCCG CCGGCTGGGC CACCAAGCTG CACATGGTTC GCGGCAAGGG CGCTGTGCTG 
CGTCCTTCGC TGGACGACCC CGTGACCATT GAGTACGTCA AGGATGTGTG GTCGAATGTG 
ACCGACATGT CCAAGGCCAA GCACTTGGGT GCCATCGCAG AGGCCTCCGG CACCCTGCTG 
GAAGTGCTTG AGAAGCTCAA GGAAGGCGGC GGCGACGCCA TCGAGGATGC CTTCGAGTTC 
AATAGCAAAG AGCTTATTAC CTACGCCCTG GGCATTGGGG CATCCGTCAA AAACGCGAAG 
GACATGCGCT TCCTGTACGA AAACGATGCC GATTTCGCCG CCATTCCAAC TTTCTTCGTT 
CTGCCCGGCC TTCTGCTGCA AATGTCCACC GACAAGCTGC TCAGCAAGGC TTTGCCCAAC 
AGCCAAGTGG ACTTCAGCAA CATTCTGCAC GGCGAACAGT ACCTAGAGAT CGTTGACGAT 
CTGCCCACCA GTGGCACTTT GCTGACCAAC GGCAAGGTCT TCGATGTTAT GGACAAGGGA 
TCCGGCGCCG TGGTCGTCAC CAATAGCGAG TCGTTCGACG AAAGTGGCCG CCTGTTGGTG 
CGCAACCAGA GCACCACTTT CATTGTCGGC GCTGGCAAAT TCGGAGGCAA GAAGGATCCC 
ATCGCTGGTG TAGTTCCGCT GCAGCCGGCG CCCAATCGCC AACCGGACGC AACCGTCCAG 
TATACGACCA GCGAGGACCA GGCGGCGCTG TACCGTCTGT CCGGCGACAA GAACCCCCTG 
CATATCGATC CCCAGATGGC CCTGCTGGCC GGCTTCAAGA CACCCATCCT CCATGGACTC 
TGCACACTGG GCTTCTCGGT GCGCGCCGTG CTCGCTCAGT TTGCGGACAA CAACCCGGCT 
CTGTTCAAGG CTGTCAAAGT GCGCTTCTCC GGACCCGTAA TTCCGGGCCA GACTTTGCGC 
GTGGACTTGT GGAAGCAGGG CACACGCATC AACTTCCGCA CCGTGGTCGT GGAGACCGGC 
AAGGAGGTCA TATCCGGCGC CTATGTCGAC CTGAAGAGCT CACAGGCCAA GCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_573109.1
CDS	226..2022
Translation	MSSSDGKLRYDGRVAVVTGAGAGLGREYALLFAERGAKVVVNDLGGTHSGDGASQRAADIVVDEIRKAGGEAVADYNSVIDGAKVIETAIKAFGRVDILVNNAGILRDRSLVKTSEQDWNLVNDVHLKGSFKCTQAAFPYMKKQNYGRIIMTSSNSGIYGNFGQVNYTAAKMGLIGLANTVAIEGARNNVLCNVIVPTAASRMTEGILPDILFNELKPKLIAPVVAYLCHESCEDNGSYIESAAGWATKLHMVRGKGAVLRPSLDDPVTIEYVKDVWSNVTDMSKAKHLGAIAEASGTLLEVLEKLKEGGGDAIEDAFEFNSKELITYALGIGASVKNAKDMRFLYENDADFAAIPTFFVLPGLLLQMSTDKLLSKALPNSQVDFSNILHGEQYLEIVDDLPTSGTLLTNGKVFDVMDKGSGAVVVTNSESFDESGRLLVRNQSTTFIVGAGKFGGKKDPIAGVVPLQPAPNRQPDATVQYTTSEDQAALYRLSGDKNPLHIDPQMALLAGFKTPILHGLCTLGFSVRAVLAQFADNNPALFKAVKVRFSGPVIPGQTLRVDLWKQGTRINFRTVVVETGKEVISGAYVDLKSSQAKL

Target ORF information:

RefSeq Version	NM_132881.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster peroxisomal multifunctional enzyme type 2, transcript variant A (Mfe2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132881.3

ORF Insert Sequence:

ATGTCCTCAT CCGATGGAAA ACTTCGTTAC GATGGCCGTG TGGCGGTGGT GACGGGAGCT 
GGTGCCGGTT TGGGCCGCGA GTACGCACTG CTCTTCGCGG AGCGTGGCGC CAAGGTGGTG 
GTCAACGATC TGGGTGGCAC CCACTCCGGC GATGGAGCAT CGCAGCGGGC CGCCGACATT 
GTGGTGGATG AGATCCGTAA GGCCGGCGGC GAGGCGGTGG CCGACTACAA CTCGGTAATC 
GATGGGGCAA AGGTCATCGA GACGGCCATC AAAGCTTTCG GACGCGTCGA TATCCTGGTC 
AACAATGCCG GCATTCTACG CGACAGGAGT CTGGTGAAGA CCAGCGAACA GGACTGGAAT 
CTGGTCAACG ATGTCCATCT GAAGGGCAGC TTTAAGTGCA CCCAGGCGGC ATTCCCGTAC 
ATGAAGAAGC AGAACTATGG CCGCATCATC ATGACATCGT CCAACTCGGG CATCTATGGT 
AATTTCGGGC AGGTCAACTA CACTGCCGCC AAGATGGGCC TCATCGGATT GGCCAACACG 
GTTGCCATCG AGGGAGCCCG CAACAATGTT CTTTGCAACG TCATCGTGCC AACTGCCGCC 
AGTCGAATGA CCGAGGGCAT TCTGCCCGAC ATTCTCTTCA ACGAGCTGAA GCCCAAGCTG 
ATTGCTCCCG TAGTGGCCTA CTTGTGCCAC GAGTCCTGCG AGGATAATGG TAGCTATATC 
GAGAGTGCCG CCGGCTGGGC CACCAAGCTG CACATGGTTC GCGGCAAGGG CGCTGTGCTG 
CGTCCTTCGC TGGACGACCC CGTGACCATT GAGTACGTCA AGGATGTGTG GTCGAATGTG 
ACCGACATGT CCAAGGCCAA GCACTTGGGT GCCATCGCAG AGGCCTCCGG CACCCTGCTG 
GAAGTGCTTG AGAAGCTCAA GGAAGGCGGC GGCGACGCCA TCGAGGATGC CTTCGAGTTC 
AATAGCAAAG AGCTTATTAC CTACGCCCTG GGCATTGGGG CATCCGTCAA AAACGCGAAG 
GACATGCGCT TCCTGTACGA AAACGATGCC GATTTCGCCG CCATTCCAAC TTTCTTCGTT 
CTGCCCGGCC TTCTGCTGCA AATGTCCACC GACAAGCTGC TCAGCAAGGC TTTGCCCAAC 
AGCCAAGTGG ACTTCAGCAA CATTCTGCAC GGCGAACAGT ACCTAGAGAT CGTTGACGAT 
CTGCCCACCA GTGGCACTTT GCTGACCAAC GGCAAGGTCT TCGATGTTAT GGACAAGGGA 
TCCGGCGCCG TGGTCGTCAC CAATAGCGAG TCGTTCGACG AAAGTGGCCG CCTGTTGGTG 
CGCAACCAGA GCACCACTTT CATTGTCGGC GCTGGCAAAT TCGGAGGCAA GAAGGATCCC 
ATCGCTGGTG TAGTTCCGCT GCAGCCGGCG CCCAATCGCC AACCGGACGC AACCGTCCAG 
TATACGACCA GCGAGGACCA GGCGGCGCTG TACCGTCTGT CCGGCGACAA GAACCCCCTG 
CATATCGATC CCCAGATGGC CCTGCTGGCC GGCTTCAAGA CACCCATCCT CCATGGACTC 
TGCACACTGG GCTTCTCGGT GCGCGCCGTG CTCGCTCAGT TTGCGGACAA CAACCCGGCT 
CTGTTCAAGG CTGTCAAAGT GCGCTTCTCC GGACCCGTAA TTCCGGGCCA GACTTTGCGC 
GTGGACTTGT GGAAGCAGGG CACACGCATC AACTTCCGCA CCGTGGTCGT GGAGACCGGC 
AAGGAGGTCA TATCCGGCGC CTATGTCGAC CTGAAGAGCT CACAGGCCAA GCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mfe2 ( NM_001298389.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285318.1 Drosophila melanogaster peroxisomal multifunctional enzyme type 2, transcript variant B (Mfe2), mRNA.

Price & Availability↑

CloneID	OFa01761
Clone ID Related Accession (Same CDS sequence)	NM_132881.3 , NM_001298389.1
Accession Version	NM_001298389.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1797bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	peroxisomal multifunctional enzyme type 2, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCTCAT CCGATGGAAA ACTTCGTTAC GATGGCCGTG TGGCGGTGGT GACGGGAGCT 
GGTGCCGGTT TGGGCCGCGA GTACGCACTG CTCTTCGCGG AGCGTGGCGC CAAGGTGGTG 
GTCAACGATC TGGGTGGCAC CCACTCCGGC GATGGAGCAT CGCAGCGGGC CGCCGACATT 
GTGGTGGATG AGATCCGTAA GGCCGGCGGC GAGGCGGTGG CCGACTACAA CTCGGTAATC 
GATGGGGCAA AGGTCATCGA GACGGCCATC AAAGCTTTCG GACGCGTCGA TATCCTGGTC 
AACAATGCCG GCATTCTACG CGACAGGAGT CTGGTGAAGA CCAGCGAACA GGACTGGAAT 
CTGGTCAACG ATGTCCATCT GAAGGGCAGC TTTAAGTGCA CCCAGGCGGC ATTCCCGTAC 
ATGAAGAAGC AGAACTATGG CCGCATCATC ATGACATCGT CCAACTCGGG CATCTATGGT 
AATTTCGGGC AGGTCAACTA CACTGCCGCC AAGATGGGCC TCATCGGATT GGCCAACACG 
GTTGCCATCG AGGGAGCCCG CAACAATGTT CTTTGCAACG TCATCGTGCC AACTGCCGCC 
AGTCGAATGA CCGAGGGCAT TCTGCCCGAC ATTCTCTTCA ACGAGCTGAA GCCCAAGCTG 
ATTGCTCCCG TAGTGGCCTA CTTGTGCCAC GAGTCCTGCG AGGATAATGG TAGCTATATC 
GAGAGTGCCG CCGGCTGGGC CACCAAGCTG CACATGGTTC GCGGCAAGGG CGCTGTGCTG 
CGTCCTTCGC TGGACGACCC CGTGACCATT GAGTACGTCA AGGATGTGTG GTCGAATGTG 
ACCGACATGT CCAAGGCCAA GCACTTGGGT GCCATCGCAG AGGCCTCCGG CACCCTGCTG 
GAAGTGCTTG AGAAGCTCAA GGAAGGCGGC GGCGACGCCA TCGAGGATGC CTTCGAGTTC 
AATAGCAAAG AGCTTATTAC CTACGCCCTG GGCATTGGGG CATCCGTCAA AAACGCGAAG 
GACATGCGCT TCCTGTACGA AAACGATGCC GATTTCGCCG CCATTCCAAC TTTCTTCGTT 
CTGCCCGGCC TTCTGCTGCA AATGTCCACC GACAAGCTGC TCAGCAAGGC TTTGCCCAAC 
AGCCAAGTGG ACTTCAGCAA CATTCTGCAC GGCGAACAGT ACCTAGAGAT CGTTGACGAT 
CTGCCCACCA GTGGCACTTT GCTGACCAAC GGCAAGGTCT TCGATGTTAT GGACAAGGGA 
TCCGGCGCCG TGGTCGTCAC CAATAGCGAG TCGTTCGACG AAAGTGGCCG CCTGTTGGTG 
CGCAACCAGA GCACCACTTT CATTGTCGGC GCTGGCAAAT TCGGAGGCAA GAAGGATCCC 
ATCGCTGGTG TAGTTCCGCT GCAGCCGGCG CCCAATCGCC AACCGGACGC AACCGTCCAG 
TATACGACCA GCGAGGACCA GGCGGCGCTG TACCGTCTGT CCGGCGACAA GAACCCCCTG 
CATATCGATC CCCAGATGGC CCTGCTGGCC GGCTTCAAGA CACCCATCCT CCATGGACTC 
TGCACACTGG GCTTCTCGGT GCGCGCCGTG CTCGCTCAGT TTGCGGACAA CAACCCGGCT 
CTGTTCAAGG CTGTCAAAGT GCGCTTCTCC GGACCCGTAA TTCCGGGCCA GACTTTGCGC 
GTGGACTTGT GGAAGCAGGG CACACGCATC AACTTCCGCA CCGTGGTCGT GGAGACCGGC 
AAGGAGGTCA TATCCGGCGC CTATGTCGAC CTGAAGAGCT CACAGGCCAA GCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285318.1
CDS	117..1913
Translation	MSSSDGKLRYDGRVAVVTGAGAGLGREYALLFAERGAKVVVNDLGGTHSGDGASQRAADIVVDEIRKAGGEAVADYNSVIDGAKVIETAIKAFGRVDILVNNAGILRDRSLVKTSEQDWNLVNDVHLKGSFKCTQAAFPYMKKQNYGRIIMTSSNSGIYGNFGQVNYTAAKMGLIGLANTVAIEGARNNVLCNVIVPTAASRMTEGILPDILFNELKPKLIAPVVAYLCHESCEDNGSYIESAAGWATKLHMVRGKGAVLRPSLDDPVTIEYVKDVWSNVTDMSKAKHLGAIAEASGTLLEVLEKLKEGGGDAIEDAFEFNSKELITYALGIGASVKNAKDMRFLYENDADFAAIPTFFVLPGLLLQMSTDKLLSKALPNSQVDFSNILHGEQYLEIVDDLPTSGTLLTNGKVFDVMDKGSGAVVVTNSESFDESGRLLVRNQSTTFIVGAGKFGGKKDPIAGVVPLQPAPNRQPDATVQYTTSEDQAALYRLSGDKNPLHIDPQMALLAGFKTPILHGLCTLGFSVRAVLAQFADNNPALFKAVKVRFSGPVIPGQTLRVDLWKQGTRINFRTVVVETGKEVISGAYVDLKSSQAKL

Target ORF information:

RefSeq Version	NM_001298389.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster peroxisomal multifunctional enzyme type 2, transcript variant B (Mfe2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298389.1

ORF Insert Sequence:

ATGTCCTCAT CCGATGGAAA ACTTCGTTAC GATGGCCGTG TGGCGGTGGT GACGGGAGCT 
GGTGCCGGTT TGGGCCGCGA GTACGCACTG CTCTTCGCGG AGCGTGGCGC CAAGGTGGTG 
GTCAACGATC TGGGTGGCAC CCACTCCGGC GATGGAGCAT CGCAGCGGGC CGCCGACATT 
GTGGTGGATG AGATCCGTAA GGCCGGCGGC GAGGCGGTGG CCGACTACAA CTCGGTAATC 
GATGGGGCAA AGGTCATCGA GACGGCCATC AAAGCTTTCG GACGCGTCGA TATCCTGGTC 
AACAATGCCG GCATTCTACG CGACAGGAGT CTGGTGAAGA CCAGCGAACA GGACTGGAAT 
CTGGTCAACG ATGTCCATCT GAAGGGCAGC TTTAAGTGCA CCCAGGCGGC ATTCCCGTAC 
ATGAAGAAGC AGAACTATGG CCGCATCATC ATGACATCGT CCAACTCGGG CATCTATGGT 
AATTTCGGGC AGGTCAACTA CACTGCCGCC AAGATGGGCC TCATCGGATT GGCCAACACG 
GTTGCCATCG AGGGAGCCCG CAACAATGTT CTTTGCAACG TCATCGTGCC AACTGCCGCC 
AGTCGAATGA CCGAGGGCAT TCTGCCCGAC ATTCTCTTCA ACGAGCTGAA GCCCAAGCTG 
ATTGCTCCCG TAGTGGCCTA CTTGTGCCAC GAGTCCTGCG AGGATAATGG TAGCTATATC 
GAGAGTGCCG CCGGCTGGGC CACCAAGCTG CACATGGTTC GCGGCAAGGG CGCTGTGCTG 
CGTCCTTCGC TGGACGACCC CGTGACCATT GAGTACGTCA AGGATGTGTG GTCGAATGTG 
ACCGACATGT CCAAGGCCAA GCACTTGGGT GCCATCGCAG AGGCCTCCGG CACCCTGCTG 
GAAGTGCTTG AGAAGCTCAA GGAAGGCGGC GGCGACGCCA TCGAGGATGC CTTCGAGTTC 
AATAGCAAAG AGCTTATTAC CTACGCCCTG GGCATTGGGG CATCCGTCAA AAACGCGAAG 
GACATGCGCT TCCTGTACGA AAACGATGCC GATTTCGCCG CCATTCCAAC TTTCTTCGTT 
CTGCCCGGCC TTCTGCTGCA AATGTCCACC GACAAGCTGC TCAGCAAGGC TTTGCCCAAC 
AGCCAAGTGG ACTTCAGCAA CATTCTGCAC GGCGAACAGT ACCTAGAGAT CGTTGACGAT 
CTGCCCACCA GTGGCACTTT GCTGACCAAC GGCAAGGTCT TCGATGTTAT GGACAAGGGA 
TCCGGCGCCG TGGTCGTCAC CAATAGCGAG TCGTTCGACG AAAGTGGCCG CCTGTTGGTG 
CGCAACCAGA GCACCACTTT CATTGTCGGC GCTGGCAAAT TCGGAGGCAA GAAGGATCCC 
ATCGCTGGTG TAGTTCCGCT GCAGCCGGCG CCCAATCGCC AACCGGACGC AACCGTCCAG 
TATACGACCA GCGAGGACCA GGCGGCGCTG TACCGTCTGT CCGGCGACAA GAACCCCCTG 
CATATCGATC CCCAGATGGC CCTGCTGGCC GGCTTCAAGA CACCCATCCT CCATGGACTC 
TGCACACTGG GCTTCTCGGT GCGCGCCGTG CTCGCTCAGT TTGCGGACAA CAACCCGGCT 
CTGTTCAAGG CTGTCAAAGT GCGCTTCTCC GGACCCGTAA TTCCGGGCCA GACTTTGCGC 
GTGGACTTGT GGAAGCAGGG CACACGCATC AACTTCCGCA CCGTGGTCGT GGAGACCGGC 
AAGGAGGTCA TATCCGGCGC CTATGTCGAC CTGAAGAGCT CACAGGCCAA GCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Mfe2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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