vap cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	vap
Entrez Gene ID	32569
Full Name	vacuolar peduncle
Synonyms	CG9209,D-RasGAP,Dmel\CG9209,FBpp0073946,RAS-GAP,RasGAP,RasGAP/Vap,RasGap,p120RasGAP,rasGap
General protein information	Preferred Names vacuolar peduncle Names CG9209 gene product from transcript CG9209-RE CG9209-PA CG9209-PB CG9209-PC CG9209-PD CG9209-PE CG9209-PF Ras GTPase-activating protein vacuolar peduncule vacuolar pedunculi vap-PA vap-PB vap-PC vap-PD vap-PE vap-PF
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 14A5-14A6\|1-54.2 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001298386.1	NP_001285315.1	vacuolar peduncle, isoform F
NM_167481.3	NP_727926.2	vacuolar peduncle, isoform A
NM_001298385.1	NP_001285314.1	vacuolar peduncle, isoform E
NM_206759.2	NP_996482.1	vacuolar peduncle, isoform D
NM_078637.3	NP_523361.1	vacuolar peduncle, isoform B
NM_167480.2	NP_727925.1	vacuolar peduncle, isoform C

Pathways from BioSystems

Homologies

	RASA1	XP_001084074.2
Xenopus tropicalis (tropical clawed frog)	LOC100135092	NP_001107303.1
Pan troglodytes (chimpanzee)	RASA1	XP_517663.3
Canis lupus familiaris (dog)	RASA1	XP_536302.3
Bos taurus (cattle)	RASA1	NP_776874.1
Mus musculus (house mouse)	Rasa1	NP_663427.2
Rattus norvegicus (Norway rat)	Rasa1	NP_037267.1
Gallus gallus (chicken)	RASA1	XP_424907.4
Danio rerio (zebrafish)	rasa1a	XP_001342009.2
Drosophila melanogaster (fruit fly)	vap	NP_727926.2
Homo sapiens (human)	RASA1	NP_002881.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002729	XP_312197.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following vap gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the vap cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01749	NM_167480.2 Latest version!	Drosophila melanogaster vacuolar peduncle (vap), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa01749	NM_078637.3 Latest version!	Drosophila melanogaster vacuolar peduncle (vap), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa01749	NM_001298386.1 Latest version!	Drosophila melanogaster vacuolar peduncle (vap), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa01749	NM_206759.2 Latest version!	Drosophila melanogaster vacuolar peduncle (vap), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa01749	NM_167481.3 Latest version!	Drosophila melanogaster vacuolar peduncle (vap), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa01749	NM_001298385.1 Latest version!	Drosophila melanogaster vacuolar peduncle (vap), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

vap ( NM_001298386.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285315.1 Drosophila melanogaster vacuolar peduncle (vap), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa01749
Clone ID Related Accession (Same CDS sequence)	NM_001298386.1 , NM_167481.3 , NM_001298385.1 , NM_206759.2 , NM_078637.3 , NM_167480.2
Accession Version	NM_001298386.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2865bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar peduncle, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGACC TAATACATTT GGGAAACATT GATGTTGTCA TCCAGAAAGA TAAGCCCGGA 
TCGCCAACGG GCTGTTCGGA TGATGGGAAT CTTGCGGGGC TGGGTGGCAT GACCGGATTG 
GGTGGCATAA TGGCACCTGG CGGCATCGAC TTGGGTGACT TGGCTCAGGA ATTGGATCAC 
GATGAATTCG ATGGACCACA TATGCTGAAT GGTGAACGTC CAGCGATTAT TGCTCCTCCG 
GAAAGCGAAT GGTACCATGG ACGACTCGAT CGCTACTCTG CAGAGTCTCG CCTACGCGGA 
AGCAGTAAAC TGGGCAGCTA TTTAGTTCGC GAAAGTGACC GCAAACCTGG CTCCTATGTT 
CTGAGTTATT ATGGCCGCAC CGGCATAAAT CATTTTCGGA TTACAGCTGT GTGCGGAGAC 
TTCTATATCG GAGGACGACA ATTCATTTCC TTAAGCGACT TGGTTGGTTA CTATACCTCA 
TGTAGTGATC TACTGAAACG CGAACGCTTG GCCATACCAG TTGCTCCGCC GGAGCCAGTT 
AATGATAAGA AACGCGTCGT AGCCATCCTG CCTTACACAA AAATGCCAGA GACCGATGAA 
CTGAGTTTCC AGAAAGGTGA TATCTTTTTT GTACACAATG ACATGGGCGA TGGCTGGTTG 
TGGGTGACTG CCCATCGTAC GGGCGAGCAG GGAATGATAT TCAGAGAACT TGTTGACGAT 
CTGGATGTAT CTATCGATCC AAATACGGTG TTTCCCTGGT TCCATCCCAA CTGCACCAAA 
AACGAAGCCG TCGATATGTT GGTTAAAGCT GGACCAGGAA GTTTCCTCGT TCGCCCAAGT 
GACAACTCAC CGGGGGATTA CTCTCTATTC TTTCATATAA ACAATCAAAT CCAACGCTTT 
CGTATTGAAA AGAAAGGCGT GCGCTATTTA ATGGGCGGTC GTACATTCGA ATGCTTGGAT 
GCGGTTATAA ATCGATATCG CAAAGAGCAA ATTGTGGAGG GCCACTCACT GAACCATCCA 
GTTGTCAATG GCATTCAGCC CGAGTTCAAT CAGCAATATG TTGTGGAAAA GGCCGCTGAA 
AAGATCTACG CAACATTGCG CGAGTGTCGT GACCAAATCG GTCTCAAAAA GATCAAGGGA 
ATCAAACATC ACGGCCATTT GAACAAGAAG TCGGACAAAA CGACGAAATG GAAGCAACTC 
TATTTCGCTT TAATCAACGA TGGTTCCGAG ACGCAACTAT GCTTCTACGA CAATCCGAAG 
AAGACCAAAC CGAAGGGTCT GATCGATTTG TCCTGTGCTT ACCTCTATCA ATGTCACGAT 
TCGCTGTGGG AGCGTCCCTA TTGCTTCCAA ATTGTCGAAA GGGCCCTGCC ATGCTTGGCC 
ACAGTGACGT ATCTGTGTGC TCCTAGTCAG GAAAGCTACG TGGAGTGGAT TAATTCGCTC 
AAGGCACAAT GCGATTCCCA GCTGAGTCGT GCCCAGAAGA AGGTATCACG CCTTCGTGAG 
CTTCGTTGTC TTAATCTGCA TGTGCTGGAA GCTCATCGAT TACCATTTAA ACTAGTGCCA 
CATCCATACT GCAGTATTTC CCTCAATCAA GTCAAAGTGG GCAAAACTCG TGTCAAAATT 
GCACCCGAAC CCGTTTGGGA AGAGGAGTTT GTGCTAGACG ATGTTCCGCC AGATGTTGTC 
TCCCTAACCA TAACATTAAT ATCGCGCGGC AAACGCGGTA AGGACTCCGA AGTGGCTGAG 
CTAACCATTG ACTTGTCTAG TCTAAAGAAT GGCCAGGAAA CCGAGGGCTG GTACCAACTC 
ACTGGCATGA CGCCGATGGG TGAATGGGGC TCGCTACGAT TGCGTATGCG CTACCTCGAC 
GATCTGATAA TGCCGTGTGA AGAGTACAGT CCTCTGCAGC AATTGCTCCT CGAATCGGAG 
CTGTATGCTG TGAAGGCGCT GGCTGAATTG TGCCATAATG ATCGGGTTCC TTTGGCCACG 
GCTCTGTTGC GAGTCTTTAG GCAGGAGAAA CGGGAAACGG AACTAATTCG AATGCTGTGC 
CAAGCGGAGG TGACGCGCGA AAACGAAACC ACAACGCTTT TCCGTGGCGC ATCCTTGGCC 
ACCACCCTAA TGGATCTATA TATGCGCACC GAATGCAGCG GCTTCCTGCA ATCGGCCGTC 
AGCGAAACCG TGCAGCGCAT TTTGGAGAGC AAGCAGTCGG CTGAATTGAA TCCCACGAAA 
ATGGACGTCA ACGATGATGC CTGTACGAAT GCCGAGTTTC TATTGCAAAT CCTCGATCTG 
GTCACCCAGT CGATATTCAC TTCACCGGAC GCCTGTCCAC GCAACGTGCG TTTCATTTGC 
AGCTGTCTTC AGAAGGCTGT GATGGCCAAA TGGCCGACAG AGCGGCTCGT TCGCACACGC 
GTCGTCTCTG GCTTTATATT CTTGCGCCTT CTGTGCCCAG CGCTGCTCAA TCCACGCCAA 
TTTGGATTGG TCAGCGAGAC GCCACCGATG GCAGCAACAC GATCTTTGGT CATGGTCGCC 
AAGTGTCTGC AGAATCTGGC CAACTTGATT GAATTCGGCG GCAAGGAACA ATACATGGAG 
GTTGTCAATC CGTTTATCTT GAAGAACAAG GAGCGTATGA TTGTTTTCTT GGATCAACTG 
TCCTCGGTGA ACGATCCGAA CCAACCGCTT GGAATGTTTG TCGAACAAAG TACAAATCTG 
AATTCACAAG ACACTGGACG CGAGCTGGCT ACCTTGCATC ACATCTGCGT ATCTCATTCG 
CAAGAACTGC ACGAGCTATC CAACATACTT TCAATTAAGA AGCTGGTCAC AGTGACGGAC 
ATGCTGACCA AACACAAGCT GAAGTATCGC GAGATGATCA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285315.1
CDS	772..3636
Translation	MADLIHLGNIDVVIQKDKPGSPTGCSDDGNLAGLGGMTGLGGIMAPGGIDLGDLAQELDHDEFDGPHMLNGERPAIIAPPESEWYHGRLDRYSAESRLRGSSKLGSYLVRESDRKPGSYVLSYYGRTGINHFRITAVCGDFYIGGRQFISLSDLVGYYTSCSDLLKRERLAIPVAPPEPVNDKKRVVAILPYTKMPETDELSFQKGDIFFVHNDMGDGWLWVTAHRTGEQGMIFRELVDDLDVSIDPNTVFPWFHPNCTKNEAVDMLVKAGPGSFLVRPSDNSPGDYSLFFHINNQIQRFRIEKKGVRYLMGGRTFECLDAVINRYRKEQIVEGHSLNHPVVNGIQPEFNQQYVVEKAAEKIYATLRECRDQIGLKKIKGIKHHGHLNKKSDKTTKWKQLYFALINDGSETQLCFYDNPKKTKPKGLIDLSCAYLYQCHDSLWERPYCFQIVERALPCLATVTYLCAPSQESYVEWINSLKAQCDSQLSRAQKKVSRLRELRCLNLHVLEAHRLPFKLVPHPYCSISLNQVKVGKTRVKIAPEPVWEEEFVLDDVPPDVVSLTITLISRGKRGKDSEVAELTIDLSSLKNGQETEGWYQLTGMTPMGEWGSLRLRMRYLDDLIMPCEEYSPLQQLLLESELYAVKALAELCHNDRVPLATALLRVFRQEKRETELIRMLCQAEVTRENETTTLFRGASLATTLMDLYMRTECSGFLQSAVSETVQRILESKQSAELNPTKMDVNDDACTNAEFLLQILDLVTQSIFTSPDACPRNVRFICSCLQKAVMAKWPTERLVRTRVVSGFIFLRLLCPALLNPRQFGLVSETPPMAATRSLVMVAKCLQNLANLIEFGGKEQYMEVVNPFILKNKERMIVFLDQLSSVNDPNQPLGMFVEQSTNLNSQDTGRELATLHHICVSHSQELHELSNILSIKKLVTVTDMLTKHKLKYREMIS

Target ORF information:

RefSeq Version	NM_001298386.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar peduncle (vap), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298386.1

ORF Insert Sequence:

ATGGCGGACC TAATACATTT GGGAAACATT GATGTTGTCA TCCAGAAAGA TAAGCCCGGA 
TCGCCAACGG GCTGTTCGGA TGATGGGAAT CTTGCGGGGC TGGGTGGCAT GACCGGATTG 
GGTGGCATAA TGGCACCTGG CGGCATCGAC TTGGGTGACT TGGCTCAGGA ATTGGATCAC 
GATGAATTCG ATGGACCACA TATGCTGAAT GGTGAACGTC CAGCGATTAT TGCTCCTCCG 
GAAAGCGAAT GGTACCATGG ACGACTCGAT CGCTACTCTG CAGAGTCTCG CCTACGCGGA 
AGCAGTAAAC TGGGCAGCTA TTTAGTTCGC GAAAGTGACC GCAAACCTGG CTCCTATGTT 
CTGAGTTATT ATGGCCGCAC CGGCATAAAT CATTTTCGGA TTACAGCTGT GTGCGGAGAC 
TTCTATATCG GAGGACGACA ATTCATTTCC TTAAGCGACT TGGTTGGTTA CTATACCTCA 
TGTAGTGATC TACTGAAACG CGAACGCTTG GCCATACCAG TTGCTCCGCC GGAGCCAGTT 
AATGATAAGA AACGCGTCGT AGCCATCCTG CCTTACACAA AAATGCCAGA GACCGATGAA 
CTGAGTTTCC AGAAAGGTGA TATCTTTTTT GTACACAATG ACATGGGCGA TGGCTGGTTG 
TGGGTGACTG CCCATCGTAC GGGCGAGCAG GGAATGATAT TCAGAGAACT TGTTGACGAT 
CTGGATGTAT CTATCGATCC AAATACGGTG TTTCCCTGGT TCCATCCCAA CTGCACCAAA 
AACGAAGCCG TCGATATGTT GGTTAAAGCT GGACCAGGAA GTTTCCTCGT TCGCCCAAGT 
GACAACTCAC CGGGGGATTA CTCTCTATTC TTTCATATAA ACAATCAAAT CCAACGCTTT 
CGTATTGAAA AGAAAGGCGT GCGCTATTTA ATGGGCGGTC GTACATTCGA ATGCTTGGAT 
GCGGTTATAA ATCGATATCG CAAAGAGCAA ATTGTGGAGG GCCACTCACT GAACCATCCA 
GTTGTCAATG GCATTCAGCC CGAGTTCAAT CAGCAATATG TTGTGGAAAA GGCCGCTGAA 
AAGATCTACG CAACATTGCG CGAGTGTCGT GACCAAATCG GTCTCAAAAA GATCAAGGGA 
ATCAAACATC ACGGCCATTT GAACAAGAAG TCGGACAAAA CGACGAAATG GAAGCAACTC 
TATTTCGCTT TAATCAACGA TGGTTCCGAG ACGCAACTAT GCTTCTACGA CAATCCGAAG 
AAGACCAAAC CGAAGGGTCT GATCGATTTG TCCTGTGCTT ACCTCTATCA ATGTCACGAT 
TCGCTGTGGG AGCGTCCCTA TTGCTTCCAA ATTGTCGAAA GGGCCCTGCC ATGCTTGGCC 
ACAGTGACGT ATCTGTGTGC TCCTAGTCAG GAAAGCTACG TGGAGTGGAT TAATTCGCTC 
AAGGCACAAT GCGATTCCCA GCTGAGTCGT GCCCAGAAGA AGGTATCACG CCTTCGTGAG 
CTTCGTTGTC TTAATCTGCA TGTGCTGGAA GCTCATCGAT TACCATTTAA ACTAGTGCCA 
CATCCATACT GCAGTATTTC CCTCAATCAA GTCAAAGTGG GCAAAACTCG TGTCAAAATT 
GCACCCGAAC CCGTTTGGGA AGAGGAGTTT GTGCTAGACG ATGTTCCGCC AGATGTTGTC 
TCCCTAACCA TAACATTAAT ATCGCGCGGC AAACGCGGTA AGGACTCCGA AGTGGCTGAG 
CTAACCATTG ACTTGTCTAG TCTAAAGAAT GGCCAGGAAA CCGAGGGCTG GTACCAACTC 
ACTGGCATGA CGCCGATGGG TGAATGGGGC TCGCTACGAT TGCGTATGCG CTACCTCGAC 
GATCTGATAA TGCCGTGTGA AGAGTACAGT CCTCTGCAGC AATTGCTCCT CGAATCGGAG 
CTGTATGCTG TGAAGGCGCT GGCTGAATTG TGCCATAATG ATCGGGTTCC TTTGGCCACG 
GCTCTGTTGC GAGTCTTTAG GCAGGAGAAA CGGGAAACGG AACTAATTCG AATGCTGTGC 
CAAGCGGAGG TGACGCGCGA AAACGAAACC ACAACGCTTT TCCGTGGCGC ATCCTTGGCC 
ACCACCCTAA TGGATCTATA TATGCGCACC GAATGCAGCG GCTTCCTGCA ATCGGCCGTC 
AGCGAAACCG TGCAGCGCAT TTTGGAGAGC AAGCAGTCGG CTGAATTGAA TCCCACGAAA 
ATGGACGTCA ACGATGATGC CTGTACGAAT GCCGAGTTTC TATTGCAAAT CCTCGATCTG 
GTCACCCAGT CGATATTCAC TTCACCGGAC GCCTGTCCAC GCAACGTGCG TTTCATTTGC 
AGCTGTCTTC AGAAGGCTGT GATGGCCAAA TGGCCGACAG AGCGGCTCGT TCGCACACGC 
GTCGTCTCTG GCTTTATATT CTTGCGCCTT CTGTGCCCAG CGCTGCTCAA TCCACGCCAA 
TTTGGATTGG TCAGCGAGAC GCCACCGATG GCAGCAACAC GATCTTTGGT CATGGTCGCC 
AAGTGTCTGC AGAATCTGGC CAACTTGATT GAATTCGGCG GCAAGGAACA ATACATGGAG 
GTTGTCAATC CGTTTATCTT GAAGAACAAG GAGCGTATGA TTGTTTTCTT GGATCAACTG 
TCCTCGGTGA ACGATCCGAA CCAACCGCTT GGAATGTTTG TCGAACAAAG TACAAATCTG 
AATTCACAAG ACACTGGACG CGAGCTGGCT ACCTTGCATC ACATCTGCGT ATCTCATTCG 
CAAGAACTGC ACGAGCTATC CAACATACTT TCAATTAAGA AGCTGGTCAC AGTGACGGAC 
ATGCTGACCA AACACAAGCT GAAGTATCGC GAGATGATCA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vap ( NM_167481.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727926.2 Drosophila melanogaster vacuolar peduncle (vap), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa01749
Clone ID Related Accession (Same CDS sequence)	NM_001298386.1 , NM_167481.3 , NM_001298385.1 , NM_206759.2 , NM_078637.3 , NM_167480.2
Accession Version	NM_167481.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2865bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar peduncle, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167481.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGACC TAATACATTT GGGAAACATT GATGTTGTCA TCCAGAAAGA TAAGCCCGGA 
TCGCCAACGG GCTGTTCGGA TGATGGGAAT CTTGCGGGGC TGGGTGGCAT GACCGGATTG 
GGTGGCATAA TGGCACCTGG CGGCATCGAC TTGGGTGACT TGGCTCAGGA ATTGGATCAC 
GATGAATTCG ATGGACCACA TATGCTGAAT GGTGAACGTC CAGCGATTAT TGCTCCTCCG 
GAAAGCGAAT GGTACCATGG ACGACTCGAT CGCTACTCTG CAGAGTCTCG CCTACGCGGA 
AGCAGTAAAC TGGGCAGCTA TTTAGTTCGC GAAAGTGACC GCAAACCTGG CTCCTATGTT 
CTGAGTTATT ATGGCCGCAC CGGCATAAAT CATTTTCGGA TTACAGCTGT GTGCGGAGAC 
TTCTATATCG GAGGACGACA ATTCATTTCC TTAAGCGACT TGGTTGGTTA CTATACCTCA 
TGTAGTGATC TACTGAAACG CGAACGCTTG GCCATACCAG TTGCTCCGCC GGAGCCAGTT 
AATGATAAGA AACGCGTCGT AGCCATCCTG CCTTACACAA AAATGCCAGA GACCGATGAA 
CTGAGTTTCC AGAAAGGTGA TATCTTTTTT GTACACAATG ACATGGGCGA TGGCTGGTTG 
TGGGTGACTG CCCATCGTAC GGGCGAGCAG GGAATGATAT TCAGAGAACT TGTTGACGAT 
CTGGATGTAT CTATCGATCC AAATACGGTG TTTCCCTGGT TCCATCCCAA CTGCACCAAA 
AACGAAGCCG TCGATATGTT GGTTAAAGCT GGACCAGGAA GTTTCCTCGT TCGCCCAAGT 
GACAACTCAC CGGGGGATTA CTCTCTATTC TTTCATATAA ACAATCAAAT CCAACGCTTT 
CGTATTGAAA AGAAAGGCGT GCGCTATTTA ATGGGCGGTC GTACATTCGA ATGCTTGGAT 
GCGGTTATAA ATCGATATCG CAAAGAGCAA ATTGTGGAGG GCCACTCACT GAACCATCCA 
GTTGTCAATG GCATTCAGCC CGAGTTCAAT CAGCAATATG TTGTGGAAAA GGCCGCTGAA 
AAGATCTACG CAACATTGCG CGAGTGTCGT GACCAAATCG GTCTCAAAAA GATCAAGGGA 
ATCAAACATC ACGGCCATTT GAACAAGAAG TCGGACAAAA CGACGAAATG GAAGCAACTC 
TATTTCGCTT TAATCAACGA TGGTTCCGAG ACGCAACTAT GCTTCTACGA CAATCCGAAG 
AAGACCAAAC CGAAGGGTCT GATCGATTTG TCCTGTGCTT ACCTCTATCA ATGTCACGAT 
TCGCTGTGGG AGCGTCCCTA TTGCTTCCAA ATTGTCGAAA GGGCCCTGCC ATGCTTGGCC 
ACAGTGACGT ATCTGTGTGC TCCTAGTCAG GAAAGCTACG TGGAGTGGAT TAATTCGCTC 
AAGGCACAAT GCGATTCCCA GCTGAGTCGT GCCCAGAAGA AGGTATCACG CCTTCGTGAG 
CTTCGTTGTC TTAATCTGCA TGTGCTGGAA GCTCATCGAT TACCATTTAA ACTAGTGCCA 
CATCCATACT GCAGTATTTC CCTCAATCAA GTCAAAGTGG GCAAAACTCG TGTCAAAATT 
GCACCCGAAC CCGTTTGGGA AGAGGAGTTT GTGCTAGACG ATGTTCCGCC AGATGTTGTC 
TCCCTAACCA TAACATTAAT ATCGCGCGGC AAACGCGGTA AGGACTCCGA AGTGGCTGAG 
CTAACCATTG ACTTGTCTAG TCTAAAGAAT GGCCAGGAAA CCGAGGGCTG GTACCAACTC 
ACTGGCATGA CGCCGATGGG TGAATGGGGC TCGCTACGAT TGCGTATGCG CTACCTCGAC 
GATCTGATAA TGCCGTGTGA AGAGTACAGT CCTCTGCAGC AATTGCTCCT CGAATCGGAG 
CTGTATGCTG TGAAGGCGCT GGCTGAATTG TGCCATAATG ATCGGGTTCC TTTGGCCACG 
GCTCTGTTGC GAGTCTTTAG GCAGGAGAAA CGGGAAACGG AACTAATTCG AATGCTGTGC 
CAAGCGGAGG TGACGCGCGA AAACGAAACC ACAACGCTTT TCCGTGGCGC ATCCTTGGCC 
ACCACCCTAA TGGATCTATA TATGCGCACC GAATGCAGCG GCTTCCTGCA ATCGGCCGTC 
AGCGAAACCG TGCAGCGCAT TTTGGAGAGC AAGCAGTCGG CTGAATTGAA TCCCACGAAA 
ATGGACGTCA ACGATGATGC CTGTACGAAT GCCGAGTTTC TATTGCAAAT CCTCGATCTG 
GTCACCCAGT CGATATTCAC TTCACCGGAC GCCTGTCCAC GCAACGTGCG TTTCATTTGC 
AGCTGTCTTC AGAAGGCTGT GATGGCCAAA TGGCCGACAG AGCGGCTCGT TCGCACACGC 
GTCGTCTCTG GCTTTATATT CTTGCGCCTT CTGTGCCCAG CGCTGCTCAA TCCACGCCAA 
TTTGGATTGG TCAGCGAGAC GCCACCGATG GCAGCAACAC GATCTTTGGT CATGGTCGCC 
AAGTGTCTGC AGAATCTGGC CAACTTGATT GAATTCGGCG GCAAGGAACA ATACATGGAG 
GTTGTCAATC CGTTTATCTT GAAGAACAAG GAGCGTATGA TTGTTTTCTT GGATCAACTG 
TCCTCGGTGA ACGATCCGAA CCAACCGCTT GGAATGTTTG TCGAACAAAG TACAAATCTG 
AATTCACAAG ACACTGGACG CGAGCTGGCT ACCTTGCATC ACATCTGCGT ATCTCATTCG 
CAAGAACTGC ACGAGCTATC CAACATACTT TCAATTAAGA AGCTGGTCAC AGTGACGGAC 
ATGCTGACCA AACACAAGCT GAAGTATCGC GAGATGATCA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727926.2
CDS	655..3519
Translation	MADLIHLGNIDVVIQKDKPGSPTGCSDDGNLAGLGGMTGLGGIMAPGGIDLGDLAQELDHDEFDGPHMLNGERPAIIAPPESEWYHGRLDRYSAESRLRGSSKLGSYLVRESDRKPGSYVLSYYGRTGINHFRITAVCGDFYIGGRQFISLSDLVGYYTSCSDLLKRERLAIPVAPPEPVNDKKRVVAILPYTKMPETDELSFQKGDIFFVHNDMGDGWLWVTAHRTGEQGMIFRELVDDLDVSIDPNTVFPWFHPNCTKNEAVDMLVKAGPGSFLVRPSDNSPGDYSLFFHINNQIQRFRIEKKGVRYLMGGRTFECLDAVINRYRKEQIVEGHSLNHPVVNGIQPEFNQQYVVEKAAEKIYATLRECRDQIGLKKIKGIKHHGHLNKKSDKTTKWKQLYFALINDGSETQLCFYDNPKKTKPKGLIDLSCAYLYQCHDSLWERPYCFQIVERALPCLATVTYLCAPSQESYVEWINSLKAQCDSQLSRAQKKVSRLRELRCLNLHVLEAHRLPFKLVPHPYCSISLNQVKVGKTRVKIAPEPVWEEEFVLDDVPPDVVSLTITLISRGKRGKDSEVAELTIDLSSLKNGQETEGWYQLTGMTPMGEWGSLRLRMRYLDDLIMPCEEYSPLQQLLLESELYAVKALAELCHNDRVPLATALLRVFRQEKRETELIRMLCQAEVTRENETTTLFRGASLATTLMDLYMRTECSGFLQSAVSETVQRILESKQSAELNPTKMDVNDDACTNAEFLLQILDLVTQSIFTSPDACPRNVRFICSCLQKAVMAKWPTERLVRTRVVSGFIFLRLLCPALLNPRQFGLVSETPPMAATRSLVMVAKCLQNLANLIEFGGKEQYMEVVNPFILKNKERMIVFLDQLSSVNDPNQPLGMFVEQSTNLNSQDTGRELATLHHICVSHSQELHELSNILSIKKLVTVTDMLTKHKLKYREMIS

Target ORF information:

RefSeq Version	NM_167481.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar peduncle (vap), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167481.3

ORF Insert Sequence:

ATGGCGGACC TAATACATTT GGGAAACATT GATGTTGTCA TCCAGAAAGA TAAGCCCGGA 
TCGCCAACGG GCTGTTCGGA TGATGGGAAT CTTGCGGGGC TGGGTGGCAT GACCGGATTG 
GGTGGCATAA TGGCACCTGG CGGCATCGAC TTGGGTGACT TGGCTCAGGA ATTGGATCAC 
GATGAATTCG ATGGACCACA TATGCTGAAT GGTGAACGTC CAGCGATTAT TGCTCCTCCG 
GAAAGCGAAT GGTACCATGG ACGACTCGAT CGCTACTCTG CAGAGTCTCG CCTACGCGGA 
AGCAGTAAAC TGGGCAGCTA TTTAGTTCGC GAAAGTGACC GCAAACCTGG CTCCTATGTT 
CTGAGTTATT ATGGCCGCAC CGGCATAAAT CATTTTCGGA TTACAGCTGT GTGCGGAGAC 
TTCTATATCG GAGGACGACA ATTCATTTCC TTAAGCGACT TGGTTGGTTA CTATACCTCA 
TGTAGTGATC TACTGAAACG CGAACGCTTG GCCATACCAG TTGCTCCGCC GGAGCCAGTT 
AATGATAAGA AACGCGTCGT AGCCATCCTG CCTTACACAA AAATGCCAGA GACCGATGAA 
CTGAGTTTCC AGAAAGGTGA TATCTTTTTT GTACACAATG ACATGGGCGA TGGCTGGTTG 
TGGGTGACTG CCCATCGTAC GGGCGAGCAG GGAATGATAT TCAGAGAACT TGTTGACGAT 
CTGGATGTAT CTATCGATCC AAATACGGTG TTTCCCTGGT TCCATCCCAA CTGCACCAAA 
AACGAAGCCG TCGATATGTT GGTTAAAGCT GGACCAGGAA GTTTCCTCGT TCGCCCAAGT 
GACAACTCAC CGGGGGATTA CTCTCTATTC TTTCATATAA ACAATCAAAT CCAACGCTTT 
CGTATTGAAA AGAAAGGCGT GCGCTATTTA ATGGGCGGTC GTACATTCGA ATGCTTGGAT 
GCGGTTATAA ATCGATATCG CAAAGAGCAA ATTGTGGAGG GCCACTCACT GAACCATCCA 
GTTGTCAATG GCATTCAGCC CGAGTTCAAT CAGCAATATG TTGTGGAAAA GGCCGCTGAA 
AAGATCTACG CAACATTGCG CGAGTGTCGT GACCAAATCG GTCTCAAAAA GATCAAGGGA 
ATCAAACATC ACGGCCATTT GAACAAGAAG TCGGACAAAA CGACGAAATG GAAGCAACTC 
TATTTCGCTT TAATCAACGA TGGTTCCGAG ACGCAACTAT GCTTCTACGA CAATCCGAAG 
AAGACCAAAC CGAAGGGTCT GATCGATTTG TCCTGTGCTT ACCTCTATCA ATGTCACGAT 
TCGCTGTGGG AGCGTCCCTA TTGCTTCCAA ATTGTCGAAA GGGCCCTGCC ATGCTTGGCC 
ACAGTGACGT ATCTGTGTGC TCCTAGTCAG GAAAGCTACG TGGAGTGGAT TAATTCGCTC 
AAGGCACAAT GCGATTCCCA GCTGAGTCGT GCCCAGAAGA AGGTATCACG CCTTCGTGAG 
CTTCGTTGTC TTAATCTGCA TGTGCTGGAA GCTCATCGAT TACCATTTAA ACTAGTGCCA 
CATCCATACT GCAGTATTTC CCTCAATCAA GTCAAAGTGG GCAAAACTCG TGTCAAAATT 
GCACCCGAAC CCGTTTGGGA AGAGGAGTTT GTGCTAGACG ATGTTCCGCC AGATGTTGTC 
TCCCTAACCA TAACATTAAT ATCGCGCGGC AAACGCGGTA AGGACTCCGA AGTGGCTGAG 
CTAACCATTG ACTTGTCTAG TCTAAAGAAT GGCCAGGAAA CCGAGGGCTG GTACCAACTC 
ACTGGCATGA CGCCGATGGG TGAATGGGGC TCGCTACGAT TGCGTATGCG CTACCTCGAC 
GATCTGATAA TGCCGTGTGA AGAGTACAGT CCTCTGCAGC AATTGCTCCT CGAATCGGAG 
CTGTATGCTG TGAAGGCGCT GGCTGAATTG TGCCATAATG ATCGGGTTCC TTTGGCCACG 
GCTCTGTTGC GAGTCTTTAG GCAGGAGAAA CGGGAAACGG AACTAATTCG AATGCTGTGC 
CAAGCGGAGG TGACGCGCGA AAACGAAACC ACAACGCTTT TCCGTGGCGC ATCCTTGGCC 
ACCACCCTAA TGGATCTATA TATGCGCACC GAATGCAGCG GCTTCCTGCA ATCGGCCGTC 
AGCGAAACCG TGCAGCGCAT TTTGGAGAGC AAGCAGTCGG CTGAATTGAA TCCCACGAAA 
ATGGACGTCA ACGATGATGC CTGTACGAAT GCCGAGTTTC TATTGCAAAT CCTCGATCTG 
GTCACCCAGT CGATATTCAC TTCACCGGAC GCCTGTCCAC GCAACGTGCG TTTCATTTGC 
AGCTGTCTTC AGAAGGCTGT GATGGCCAAA TGGCCGACAG AGCGGCTCGT TCGCACACGC 
GTCGTCTCTG GCTTTATATT CTTGCGCCTT CTGTGCCCAG CGCTGCTCAA TCCACGCCAA 
TTTGGATTGG TCAGCGAGAC GCCACCGATG GCAGCAACAC GATCTTTGGT CATGGTCGCC 
AAGTGTCTGC AGAATCTGGC CAACTTGATT GAATTCGGCG GCAAGGAACA ATACATGGAG 
GTTGTCAATC CGTTTATCTT GAAGAACAAG GAGCGTATGA TTGTTTTCTT GGATCAACTG 
TCCTCGGTGA ACGATCCGAA CCAACCGCTT GGAATGTTTG TCGAACAAAG TACAAATCTG 
AATTCACAAG ACACTGGACG CGAGCTGGCT ACCTTGCATC ACATCTGCGT ATCTCATTCG 
CAAGAACTGC ACGAGCTATC CAACATACTT TCAATTAAGA AGCTGGTCAC AGTGACGGAC 
ATGCTGACCA AACACAAGCT GAAGTATCGC GAGATGATCA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vap ( NM_001298385.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285314.1 Drosophila melanogaster vacuolar peduncle (vap), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa01749
Clone ID Related Accession (Same CDS sequence)	NM_001298386.1 , NM_167481.3 , NM_001298385.1 , NM_206759.2 , NM_078637.3 , NM_167480.2
Accession Version	NM_001298385.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2865bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar peduncle, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGACC TAATACATTT GGGAAACATT GATGTTGTCA TCCAGAAAGA TAAGCCCGGA 
TCGCCAACGG GCTGTTCGGA TGATGGGAAT CTTGCGGGGC TGGGTGGCAT GACCGGATTG 
GGTGGCATAA TGGCACCTGG CGGCATCGAC TTGGGTGACT TGGCTCAGGA ATTGGATCAC 
GATGAATTCG ATGGACCACA TATGCTGAAT GGTGAACGTC CAGCGATTAT TGCTCCTCCG 
GAAAGCGAAT GGTACCATGG ACGACTCGAT CGCTACTCTG CAGAGTCTCG CCTACGCGGA 
AGCAGTAAAC TGGGCAGCTA TTTAGTTCGC GAAAGTGACC GCAAACCTGG CTCCTATGTT 
CTGAGTTATT ATGGCCGCAC CGGCATAAAT CATTTTCGGA TTACAGCTGT GTGCGGAGAC 
TTCTATATCG GAGGACGACA ATTCATTTCC TTAAGCGACT TGGTTGGTTA CTATACCTCA 
TGTAGTGATC TACTGAAACG CGAACGCTTG GCCATACCAG TTGCTCCGCC GGAGCCAGTT 
AATGATAAGA AACGCGTCGT AGCCATCCTG CCTTACACAA AAATGCCAGA GACCGATGAA 
CTGAGTTTCC AGAAAGGTGA TATCTTTTTT GTACACAATG ACATGGGCGA TGGCTGGTTG 
TGGGTGACTG CCCATCGTAC GGGCGAGCAG GGAATGATAT TCAGAGAACT TGTTGACGAT 
CTGGATGTAT CTATCGATCC AAATACGGTG TTTCCCTGGT TCCATCCCAA CTGCACCAAA 
AACGAAGCCG TCGATATGTT GGTTAAAGCT GGACCAGGAA GTTTCCTCGT TCGCCCAAGT 
GACAACTCAC CGGGGGATTA CTCTCTATTC TTTCATATAA ACAATCAAAT CCAACGCTTT 
CGTATTGAAA AGAAAGGCGT GCGCTATTTA ATGGGCGGTC GTACATTCGA ATGCTTGGAT 
GCGGTTATAA ATCGATATCG CAAAGAGCAA ATTGTGGAGG GCCACTCACT GAACCATCCA 
GTTGTCAATG GCATTCAGCC CGAGTTCAAT CAGCAATATG TTGTGGAAAA GGCCGCTGAA 
AAGATCTACG CAACATTGCG CGAGTGTCGT GACCAAATCG GTCTCAAAAA GATCAAGGGA 
ATCAAACATC ACGGCCATTT GAACAAGAAG TCGGACAAAA CGACGAAATG GAAGCAACTC 
TATTTCGCTT TAATCAACGA TGGTTCCGAG ACGCAACTAT GCTTCTACGA CAATCCGAAG 
AAGACCAAAC CGAAGGGTCT GATCGATTTG TCCTGTGCTT ACCTCTATCA ATGTCACGAT 
TCGCTGTGGG AGCGTCCCTA TTGCTTCCAA ATTGTCGAAA GGGCCCTGCC ATGCTTGGCC 
ACAGTGACGT ATCTGTGTGC TCCTAGTCAG GAAAGCTACG TGGAGTGGAT TAATTCGCTC 
AAGGCACAAT GCGATTCCCA GCTGAGTCGT GCCCAGAAGA AGGTATCACG CCTTCGTGAG 
CTTCGTTGTC TTAATCTGCA TGTGCTGGAA GCTCATCGAT TACCATTTAA ACTAGTGCCA 
CATCCATACT GCAGTATTTC CCTCAATCAA GTCAAAGTGG GCAAAACTCG TGTCAAAATT 
GCACCCGAAC CCGTTTGGGA AGAGGAGTTT GTGCTAGACG ATGTTCCGCC AGATGTTGTC 
TCCCTAACCA TAACATTAAT ATCGCGCGGC AAACGCGGTA AGGACTCCGA AGTGGCTGAG 
CTAACCATTG ACTTGTCTAG TCTAAAGAAT GGCCAGGAAA CCGAGGGCTG GTACCAACTC 
ACTGGCATGA CGCCGATGGG TGAATGGGGC TCGCTACGAT TGCGTATGCG CTACCTCGAC 
GATCTGATAA TGCCGTGTGA AGAGTACAGT CCTCTGCAGC AATTGCTCCT CGAATCGGAG 
CTGTATGCTG TGAAGGCGCT GGCTGAATTG TGCCATAATG ATCGGGTTCC TTTGGCCACG 
GCTCTGTTGC GAGTCTTTAG GCAGGAGAAA CGGGAAACGG AACTAATTCG AATGCTGTGC 
CAAGCGGAGG TGACGCGCGA AAACGAAACC ACAACGCTTT TCCGTGGCGC ATCCTTGGCC 
ACCACCCTAA TGGATCTATA TATGCGCACC GAATGCAGCG GCTTCCTGCA ATCGGCCGTC 
AGCGAAACCG TGCAGCGCAT TTTGGAGAGC AAGCAGTCGG CTGAATTGAA TCCCACGAAA 
ATGGACGTCA ACGATGATGC CTGTACGAAT GCCGAGTTTC TATTGCAAAT CCTCGATCTG 
GTCACCCAGT CGATATTCAC TTCACCGGAC GCCTGTCCAC GCAACGTGCG TTTCATTTGC 
AGCTGTCTTC AGAAGGCTGT GATGGCCAAA TGGCCGACAG AGCGGCTCGT TCGCACACGC 
GTCGTCTCTG GCTTTATATT CTTGCGCCTT CTGTGCCCAG CGCTGCTCAA TCCACGCCAA 
TTTGGATTGG TCAGCGAGAC GCCACCGATG GCAGCAACAC GATCTTTGGT CATGGTCGCC 
AAGTGTCTGC AGAATCTGGC CAACTTGATT GAATTCGGCG GCAAGGAACA ATACATGGAG 
GTTGTCAATC CGTTTATCTT GAAGAACAAG GAGCGTATGA TTGTTTTCTT GGATCAACTG 
TCCTCGGTGA ACGATCCGAA CCAACCGCTT GGAATGTTTG TCGAACAAAG TACAAATCTG 
AATTCACAAG ACACTGGACG CGAGCTGGCT ACCTTGCATC ACATCTGCGT ATCTCATTCG 
CAAGAACTGC ACGAGCTATC CAACATACTT TCAATTAAGA AGCTGGTCAC AGTGACGGAC 
ATGCTGACCA AACACAAGCT GAAGTATCGC GAGATGATCA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285314.1
CDS	990..3854
Translation	MADLIHLGNIDVVIQKDKPGSPTGCSDDGNLAGLGGMTGLGGIMAPGGIDLGDLAQELDHDEFDGPHMLNGERPAIIAPPESEWYHGRLDRYSAESRLRGSSKLGSYLVRESDRKPGSYVLSYYGRTGINHFRITAVCGDFYIGGRQFISLSDLVGYYTSCSDLLKRERLAIPVAPPEPVNDKKRVVAILPYTKMPETDELSFQKGDIFFVHNDMGDGWLWVTAHRTGEQGMIFRELVDDLDVSIDPNTVFPWFHPNCTKNEAVDMLVKAGPGSFLVRPSDNSPGDYSLFFHINNQIQRFRIEKKGVRYLMGGRTFECLDAVINRYRKEQIVEGHSLNHPVVNGIQPEFNQQYVVEKAAEKIYATLRECRDQIGLKKIKGIKHHGHLNKKSDKTTKWKQLYFALINDGSETQLCFYDNPKKTKPKGLIDLSCAYLYQCHDSLWERPYCFQIVERALPCLATVTYLCAPSQESYVEWINSLKAQCDSQLSRAQKKVSRLRELRCLNLHVLEAHRLPFKLVPHPYCSISLNQVKVGKTRVKIAPEPVWEEEFVLDDVPPDVVSLTITLISRGKRGKDSEVAELTIDLSSLKNGQETEGWYQLTGMTPMGEWGSLRLRMRYLDDLIMPCEEYSPLQQLLLESELYAVKALAELCHNDRVPLATALLRVFRQEKRETELIRMLCQAEVTRENETTTLFRGASLATTLMDLYMRTECSGFLQSAVSETVQRILESKQSAELNPTKMDVNDDACTNAEFLLQILDLVTQSIFTSPDACPRNVRFICSCLQKAVMAKWPTERLVRTRVVSGFIFLRLLCPALLNPRQFGLVSETPPMAATRSLVMVAKCLQNLANLIEFGGKEQYMEVVNPFILKNKERMIVFLDQLSSVNDPNQPLGMFVEQSTNLNSQDTGRELATLHHICVSHSQELHELSNILSIKKLVTVTDMLTKHKLKYREMIS

Target ORF information:

RefSeq Version	NM_001298385.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar peduncle (vap), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298385.1

ORF Insert Sequence:

ATGGCGGACC TAATACATTT GGGAAACATT GATGTTGTCA TCCAGAAAGA TAAGCCCGGA 
TCGCCAACGG GCTGTTCGGA TGATGGGAAT CTTGCGGGGC TGGGTGGCAT GACCGGATTG 
GGTGGCATAA TGGCACCTGG CGGCATCGAC TTGGGTGACT TGGCTCAGGA ATTGGATCAC 
GATGAATTCG ATGGACCACA TATGCTGAAT GGTGAACGTC CAGCGATTAT TGCTCCTCCG 
GAAAGCGAAT GGTACCATGG ACGACTCGAT CGCTACTCTG CAGAGTCTCG CCTACGCGGA 
AGCAGTAAAC TGGGCAGCTA TTTAGTTCGC GAAAGTGACC GCAAACCTGG CTCCTATGTT 
CTGAGTTATT ATGGCCGCAC CGGCATAAAT CATTTTCGGA TTACAGCTGT GTGCGGAGAC 
TTCTATATCG GAGGACGACA ATTCATTTCC TTAAGCGACT TGGTTGGTTA CTATACCTCA 
TGTAGTGATC TACTGAAACG CGAACGCTTG GCCATACCAG TTGCTCCGCC GGAGCCAGTT 
AATGATAAGA AACGCGTCGT AGCCATCCTG CCTTACACAA AAATGCCAGA GACCGATGAA 
CTGAGTTTCC AGAAAGGTGA TATCTTTTTT GTACACAATG ACATGGGCGA TGGCTGGTTG 
TGGGTGACTG CCCATCGTAC GGGCGAGCAG GGAATGATAT TCAGAGAACT TGTTGACGAT 
CTGGATGTAT CTATCGATCC AAATACGGTG TTTCCCTGGT TCCATCCCAA CTGCACCAAA 
AACGAAGCCG TCGATATGTT GGTTAAAGCT GGACCAGGAA GTTTCCTCGT TCGCCCAAGT 
GACAACTCAC CGGGGGATTA CTCTCTATTC TTTCATATAA ACAATCAAAT CCAACGCTTT 
CGTATTGAAA AGAAAGGCGT GCGCTATTTA ATGGGCGGTC GTACATTCGA ATGCTTGGAT 
GCGGTTATAA ATCGATATCG CAAAGAGCAA ATTGTGGAGG GCCACTCACT GAACCATCCA 
GTTGTCAATG GCATTCAGCC CGAGTTCAAT CAGCAATATG TTGTGGAAAA GGCCGCTGAA 
AAGATCTACG CAACATTGCG CGAGTGTCGT GACCAAATCG GTCTCAAAAA GATCAAGGGA 
ATCAAACATC ACGGCCATTT GAACAAGAAG TCGGACAAAA CGACGAAATG GAAGCAACTC 
TATTTCGCTT TAATCAACGA TGGTTCCGAG ACGCAACTAT GCTTCTACGA CAATCCGAAG 
AAGACCAAAC CGAAGGGTCT GATCGATTTG TCCTGTGCTT ACCTCTATCA ATGTCACGAT 
TCGCTGTGGG AGCGTCCCTA TTGCTTCCAA ATTGTCGAAA GGGCCCTGCC ATGCTTGGCC 
ACAGTGACGT ATCTGTGTGC TCCTAGTCAG GAAAGCTACG TGGAGTGGAT TAATTCGCTC 
AAGGCACAAT GCGATTCCCA GCTGAGTCGT GCCCAGAAGA AGGTATCACG CCTTCGTGAG 
CTTCGTTGTC TTAATCTGCA TGTGCTGGAA GCTCATCGAT TACCATTTAA ACTAGTGCCA 
CATCCATACT GCAGTATTTC CCTCAATCAA GTCAAAGTGG GCAAAACTCG TGTCAAAATT 
GCACCCGAAC CCGTTTGGGA AGAGGAGTTT GTGCTAGACG ATGTTCCGCC AGATGTTGTC 
TCCCTAACCA TAACATTAAT ATCGCGCGGC AAACGCGGTA AGGACTCCGA AGTGGCTGAG 
CTAACCATTG ACTTGTCTAG TCTAAAGAAT GGCCAGGAAA CCGAGGGCTG GTACCAACTC 
ACTGGCATGA CGCCGATGGG TGAATGGGGC TCGCTACGAT TGCGTATGCG CTACCTCGAC 
GATCTGATAA TGCCGTGTGA AGAGTACAGT CCTCTGCAGC AATTGCTCCT CGAATCGGAG 
CTGTATGCTG TGAAGGCGCT GGCTGAATTG TGCCATAATG ATCGGGTTCC TTTGGCCACG 
GCTCTGTTGC GAGTCTTTAG GCAGGAGAAA CGGGAAACGG AACTAATTCG AATGCTGTGC 
CAAGCGGAGG TGACGCGCGA AAACGAAACC ACAACGCTTT TCCGTGGCGC ATCCTTGGCC 
ACCACCCTAA TGGATCTATA TATGCGCACC GAATGCAGCG GCTTCCTGCA ATCGGCCGTC 
AGCGAAACCG TGCAGCGCAT TTTGGAGAGC AAGCAGTCGG CTGAATTGAA TCCCACGAAA 
ATGGACGTCA ACGATGATGC CTGTACGAAT GCCGAGTTTC TATTGCAAAT CCTCGATCTG 
GTCACCCAGT CGATATTCAC TTCACCGGAC GCCTGTCCAC GCAACGTGCG TTTCATTTGC 
AGCTGTCTTC AGAAGGCTGT GATGGCCAAA TGGCCGACAG AGCGGCTCGT TCGCACACGC 
GTCGTCTCTG GCTTTATATT CTTGCGCCTT CTGTGCCCAG CGCTGCTCAA TCCACGCCAA 
TTTGGATTGG TCAGCGAGAC GCCACCGATG GCAGCAACAC GATCTTTGGT CATGGTCGCC 
AAGTGTCTGC AGAATCTGGC CAACTTGATT GAATTCGGCG GCAAGGAACA ATACATGGAG 
GTTGTCAATC CGTTTATCTT GAAGAACAAG GAGCGTATGA TTGTTTTCTT GGATCAACTG 
TCCTCGGTGA ACGATCCGAA CCAACCGCTT GGAATGTTTG TCGAACAAAG TACAAATCTG 
AATTCACAAG ACACTGGACG CGAGCTGGCT ACCTTGCATC ACATCTGCGT ATCTCATTCG 
CAAGAACTGC ACGAGCTATC CAACATACTT TCAATTAAGA AGCTGGTCAC AGTGACGGAC 
ATGCTGACCA AACACAAGCT GAAGTATCGC GAGATGATCA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vap ( NM_206759.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996482.1 Drosophila melanogaster vacuolar peduncle (vap), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa01749
Clone ID Related Accession (Same CDS sequence)	NM_001298386.1 , NM_167481.3 , NM_001298385.1 , NM_206759.2 , NM_078637.3 , NM_167480.2
Accession Version	NM_206759.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2865bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar peduncle, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_206759.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGACC TAATACATTT GGGAAACATT GATGTTGTCA TCCAGAAAGA TAAGCCCGGA 
TCGCCAACGG GCTGTTCGGA TGATGGGAAT CTTGCGGGGC TGGGTGGCAT GACCGGATTG 
GGTGGCATAA TGGCACCTGG CGGCATCGAC TTGGGTGACT TGGCTCAGGA ATTGGATCAC 
GATGAATTCG ATGGACCACA TATGCTGAAT GGTGAACGTC CAGCGATTAT TGCTCCTCCG 
GAAAGCGAAT GGTACCATGG ACGACTCGAT CGCTACTCTG CAGAGTCTCG CCTACGCGGA 
AGCAGTAAAC TGGGCAGCTA TTTAGTTCGC GAAAGTGACC GCAAACCTGG CTCCTATGTT 
CTGAGTTATT ATGGCCGCAC CGGCATAAAT CATTTTCGGA TTACAGCTGT GTGCGGAGAC 
TTCTATATCG GAGGACGACA ATTCATTTCC TTAAGCGACT TGGTTGGTTA CTATACCTCA 
TGTAGTGATC TACTGAAACG CGAACGCTTG GCCATACCAG TTGCTCCGCC GGAGCCAGTT 
AATGATAAGA AACGCGTCGT AGCCATCCTG CCTTACACAA AAATGCCAGA GACCGATGAA 
CTGAGTTTCC AGAAAGGTGA TATCTTTTTT GTACACAATG ACATGGGCGA TGGCTGGTTG 
TGGGTGACTG CCCATCGTAC GGGCGAGCAG GGAATGATAT TCAGAGAACT TGTTGACGAT 
CTGGATGTAT CTATCGATCC AAATACGGTG TTTCCCTGGT TCCATCCCAA CTGCACCAAA 
AACGAAGCCG TCGATATGTT GGTTAAAGCT GGACCAGGAA GTTTCCTCGT TCGCCCAAGT 
GACAACTCAC CGGGGGATTA CTCTCTATTC TTTCATATAA ACAATCAAAT CCAACGCTTT 
CGTATTGAAA AGAAAGGCGT GCGCTATTTA ATGGGCGGTC GTACATTCGA ATGCTTGGAT 
GCGGTTATAA ATCGATATCG CAAAGAGCAA ATTGTGGAGG GCCACTCACT GAACCATCCA 
GTTGTCAATG GCATTCAGCC CGAGTTCAAT CAGCAATATG TTGTGGAAAA GGCCGCTGAA 
AAGATCTACG CAACATTGCG CGAGTGTCGT GACCAAATCG GTCTCAAAAA GATCAAGGGA 
ATCAAACATC ACGGCCATTT GAACAAGAAG TCGGACAAAA CGACGAAATG GAAGCAACTC 
TATTTCGCTT TAATCAACGA TGGTTCCGAG ACGCAACTAT GCTTCTACGA CAATCCGAAG 
AAGACCAAAC CGAAGGGTCT GATCGATTTG TCCTGTGCTT ACCTCTATCA ATGTCACGAT 
TCGCTGTGGG AGCGTCCCTA TTGCTTCCAA ATTGTCGAAA GGGCCCTGCC ATGCTTGGCC 
ACAGTGACGT ATCTGTGTGC TCCTAGTCAG GAAAGCTACG TGGAGTGGAT TAATTCGCTC 
AAGGCACAAT GCGATTCCCA GCTGAGTCGT GCCCAGAAGA AGGTATCACG CCTTCGTGAG 
CTTCGTTGTC TTAATCTGCA TGTGCTGGAA GCTCATCGAT TACCATTTAA ACTAGTGCCA 
CATCCATACT GCAGTATTTC CCTCAATCAA GTCAAAGTGG GCAAAACTCG TGTCAAAATT 
GCACCCGAAC CCGTTTGGGA AGAGGAGTTT GTGCTAGACG ATGTTCCGCC AGATGTTGTC 
TCCCTAACCA TAACATTAAT ATCGCGCGGC AAACGCGGTA AGGACTCCGA AGTGGCTGAG 
CTAACCATTG ACTTGTCTAG TCTAAAGAAT GGCCAGGAAA CCGAGGGCTG GTACCAACTC 
ACTGGCATGA CGCCGATGGG TGAATGGGGC TCGCTACGAT TGCGTATGCG CTACCTCGAC 
GATCTGATAA TGCCGTGTGA AGAGTACAGT CCTCTGCAGC AATTGCTCCT CGAATCGGAG 
CTGTATGCTG TGAAGGCGCT GGCTGAATTG TGCCATAATG ATCGGGTTCC TTTGGCCACG 
GCTCTGTTGC GAGTCTTTAG GCAGGAGAAA CGGGAAACGG AACTAATTCG AATGCTGTGC 
CAAGCGGAGG TGACGCGCGA AAACGAAACC ACAACGCTTT TCCGTGGCGC ATCCTTGGCC 
ACCACCCTAA TGGATCTATA TATGCGCACC GAATGCAGCG GCTTCCTGCA ATCGGCCGTC 
AGCGAAACCG TGCAGCGCAT TTTGGAGAGC AAGCAGTCGG CTGAATTGAA TCCCACGAAA 
ATGGACGTCA ACGATGATGC CTGTACGAAT GCCGAGTTTC TATTGCAAAT CCTCGATCTG 
GTCACCCAGT CGATATTCAC TTCACCGGAC GCCTGTCCAC GCAACGTGCG TTTCATTTGC 
AGCTGTCTTC AGAAGGCTGT GATGGCCAAA TGGCCGACAG AGCGGCTCGT TCGCACACGC 
GTCGTCTCTG GCTTTATATT CTTGCGCCTT CTGTGCCCAG CGCTGCTCAA TCCACGCCAA 
TTTGGATTGG TCAGCGAGAC GCCACCGATG GCAGCAACAC GATCTTTGGT CATGGTCGCC 
AAGTGTCTGC AGAATCTGGC CAACTTGATT GAATTCGGCG GCAAGGAACA ATACATGGAG 
GTTGTCAATC CGTTTATCTT GAAGAACAAG GAGCGTATGA TTGTTTTCTT GGATCAACTG 
TCCTCGGTGA ACGATCCGAA CCAACCGCTT GGAATGTTTG TCGAACAAAG TACAAATCTG 
AATTCACAAG ACACTGGACG CGAGCTGGCT ACCTTGCATC ACATCTGCGT ATCTCATTCG 
CAAGAACTGC ACGAGCTATC CAACATACTT TCAATTAAGA AGCTGGTCAC AGTGACGGAC 
ATGCTGACCA AACACAAGCT GAAGTATCGC GAGATGATCA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996482.1
CDS	576..3440
Translation	MADLIHLGNIDVVIQKDKPGSPTGCSDDGNLAGLGGMTGLGGIMAPGGIDLGDLAQELDHDEFDGPHMLNGERPAIIAPPESEWYHGRLDRYSAESRLRGSSKLGSYLVRESDRKPGSYVLSYYGRTGINHFRITAVCGDFYIGGRQFISLSDLVGYYTSCSDLLKRERLAIPVAPPEPVNDKKRVVAILPYTKMPETDELSFQKGDIFFVHNDMGDGWLWVTAHRTGEQGMIFRELVDDLDVSIDPNTVFPWFHPNCTKNEAVDMLVKAGPGSFLVRPSDNSPGDYSLFFHINNQIQRFRIEKKGVRYLMGGRTFECLDAVINRYRKEQIVEGHSLNHPVVNGIQPEFNQQYVVEKAAEKIYATLRECRDQIGLKKIKGIKHHGHLNKKSDKTTKWKQLYFALINDGSETQLCFYDNPKKTKPKGLIDLSCAYLYQCHDSLWERPYCFQIVERALPCLATVTYLCAPSQESYVEWINSLKAQCDSQLSRAQKKVSRLRELRCLNLHVLEAHRLPFKLVPHPYCSISLNQVKVGKTRVKIAPEPVWEEEFVLDDVPPDVVSLTITLISRGKRGKDSEVAELTIDLSSLKNGQETEGWYQLTGMTPMGEWGSLRLRMRYLDDLIMPCEEYSPLQQLLLESELYAVKALAELCHNDRVPLATALLRVFRQEKRETELIRMLCQAEVTRENETTTLFRGASLATTLMDLYMRTECSGFLQSAVSETVQRILESKQSAELNPTKMDVNDDACTNAEFLLQILDLVTQSIFTSPDACPRNVRFICSCLQKAVMAKWPTERLVRTRVVSGFIFLRLLCPALLNPRQFGLVSETPPMAATRSLVMVAKCLQNLANLIEFGGKEQYMEVVNPFILKNKERMIVFLDQLSSVNDPNQPLGMFVEQSTNLNSQDTGRELATLHHICVSHSQELHELSNILSIKKLVTVTDMLTKHKLKYREMIS

Target ORF information:

RefSeq Version	NM_206759.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar peduncle (vap), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206759.2

ORF Insert Sequence:

ATGGCGGACC TAATACATTT GGGAAACATT GATGTTGTCA TCCAGAAAGA TAAGCCCGGA 
TCGCCAACGG GCTGTTCGGA TGATGGGAAT CTTGCGGGGC TGGGTGGCAT GACCGGATTG 
GGTGGCATAA TGGCACCTGG CGGCATCGAC TTGGGTGACT TGGCTCAGGA ATTGGATCAC 
GATGAATTCG ATGGACCACA TATGCTGAAT GGTGAACGTC CAGCGATTAT TGCTCCTCCG 
GAAAGCGAAT GGTACCATGG ACGACTCGAT CGCTACTCTG CAGAGTCTCG CCTACGCGGA 
AGCAGTAAAC TGGGCAGCTA TTTAGTTCGC GAAAGTGACC GCAAACCTGG CTCCTATGTT 
CTGAGTTATT ATGGCCGCAC CGGCATAAAT CATTTTCGGA TTACAGCTGT GTGCGGAGAC 
TTCTATATCG GAGGACGACA ATTCATTTCC TTAAGCGACT TGGTTGGTTA CTATACCTCA 
TGTAGTGATC TACTGAAACG CGAACGCTTG GCCATACCAG TTGCTCCGCC GGAGCCAGTT 
AATGATAAGA AACGCGTCGT AGCCATCCTG CCTTACACAA AAATGCCAGA GACCGATGAA 
CTGAGTTTCC AGAAAGGTGA TATCTTTTTT GTACACAATG ACATGGGCGA TGGCTGGTTG 
TGGGTGACTG CCCATCGTAC GGGCGAGCAG GGAATGATAT TCAGAGAACT TGTTGACGAT 
CTGGATGTAT CTATCGATCC AAATACGGTG TTTCCCTGGT TCCATCCCAA CTGCACCAAA 
AACGAAGCCG TCGATATGTT GGTTAAAGCT GGACCAGGAA GTTTCCTCGT TCGCCCAAGT 
GACAACTCAC CGGGGGATTA CTCTCTATTC TTTCATATAA ACAATCAAAT CCAACGCTTT 
CGTATTGAAA AGAAAGGCGT GCGCTATTTA ATGGGCGGTC GTACATTCGA ATGCTTGGAT 
GCGGTTATAA ATCGATATCG CAAAGAGCAA ATTGTGGAGG GCCACTCACT GAACCATCCA 
GTTGTCAATG GCATTCAGCC CGAGTTCAAT CAGCAATATG TTGTGGAAAA GGCCGCTGAA 
AAGATCTACG CAACATTGCG CGAGTGTCGT GACCAAATCG GTCTCAAAAA GATCAAGGGA 
ATCAAACATC ACGGCCATTT GAACAAGAAG TCGGACAAAA CGACGAAATG GAAGCAACTC 
TATTTCGCTT TAATCAACGA TGGTTCCGAG ACGCAACTAT GCTTCTACGA CAATCCGAAG 
AAGACCAAAC CGAAGGGTCT GATCGATTTG TCCTGTGCTT ACCTCTATCA ATGTCACGAT 
TCGCTGTGGG AGCGTCCCTA TTGCTTCCAA ATTGTCGAAA GGGCCCTGCC ATGCTTGGCC 
ACAGTGACGT ATCTGTGTGC TCCTAGTCAG GAAAGCTACG TGGAGTGGAT TAATTCGCTC 
AAGGCACAAT GCGATTCCCA GCTGAGTCGT GCCCAGAAGA AGGTATCACG CCTTCGTGAG 
CTTCGTTGTC TTAATCTGCA TGTGCTGGAA GCTCATCGAT TACCATTTAA ACTAGTGCCA 
CATCCATACT GCAGTATTTC CCTCAATCAA GTCAAAGTGG GCAAAACTCG TGTCAAAATT 
GCACCCGAAC CCGTTTGGGA AGAGGAGTTT GTGCTAGACG ATGTTCCGCC AGATGTTGTC 
TCCCTAACCA TAACATTAAT ATCGCGCGGC AAACGCGGTA AGGACTCCGA AGTGGCTGAG 
CTAACCATTG ACTTGTCTAG TCTAAAGAAT GGCCAGGAAA CCGAGGGCTG GTACCAACTC 
ACTGGCATGA CGCCGATGGG TGAATGGGGC TCGCTACGAT TGCGTATGCG CTACCTCGAC 
GATCTGATAA TGCCGTGTGA AGAGTACAGT CCTCTGCAGC AATTGCTCCT CGAATCGGAG 
CTGTATGCTG TGAAGGCGCT GGCTGAATTG TGCCATAATG ATCGGGTTCC TTTGGCCACG 
GCTCTGTTGC GAGTCTTTAG GCAGGAGAAA CGGGAAACGG AACTAATTCG AATGCTGTGC 
CAAGCGGAGG TGACGCGCGA AAACGAAACC ACAACGCTTT TCCGTGGCGC ATCCTTGGCC 
ACCACCCTAA TGGATCTATA TATGCGCACC GAATGCAGCG GCTTCCTGCA ATCGGCCGTC 
AGCGAAACCG TGCAGCGCAT TTTGGAGAGC AAGCAGTCGG CTGAATTGAA TCCCACGAAA 
ATGGACGTCA ACGATGATGC CTGTACGAAT GCCGAGTTTC TATTGCAAAT CCTCGATCTG 
GTCACCCAGT CGATATTCAC TTCACCGGAC GCCTGTCCAC GCAACGTGCG TTTCATTTGC 
AGCTGTCTTC AGAAGGCTGT GATGGCCAAA TGGCCGACAG AGCGGCTCGT TCGCACACGC 
GTCGTCTCTG GCTTTATATT CTTGCGCCTT CTGTGCCCAG CGCTGCTCAA TCCACGCCAA 
TTTGGATTGG TCAGCGAGAC GCCACCGATG GCAGCAACAC GATCTTTGGT CATGGTCGCC 
AAGTGTCTGC AGAATCTGGC CAACTTGATT GAATTCGGCG GCAAGGAACA ATACATGGAG 
GTTGTCAATC CGTTTATCTT GAAGAACAAG GAGCGTATGA TTGTTTTCTT GGATCAACTG 
TCCTCGGTGA ACGATCCGAA CCAACCGCTT GGAATGTTTG TCGAACAAAG TACAAATCTG 
AATTCACAAG ACACTGGACG CGAGCTGGCT ACCTTGCATC ACATCTGCGT ATCTCATTCG 
CAAGAACTGC ACGAGCTATC CAACATACTT TCAATTAAGA AGCTGGTCAC AGTGACGGAC 
ATGCTGACCA AACACAAGCT GAAGTATCGC GAGATGATCA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vap ( NM_078637.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523361.1 Drosophila melanogaster vacuolar peduncle (vap), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa01749
Clone ID Related Accession (Same CDS sequence)	NM_001298386.1 , NM_167481.3 , NM_001298385.1 , NM_206759.2 , NM_078637.3 , NM_167480.2
Accession Version	NM_078637.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2865bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar peduncle, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_078637.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGACC TAATACATTT GGGAAACATT GATGTTGTCA TCCAGAAAGA TAAGCCCGGA 
TCGCCAACGG GCTGTTCGGA TGATGGGAAT CTTGCGGGGC TGGGTGGCAT GACCGGATTG 
GGTGGCATAA TGGCACCTGG CGGCATCGAC TTGGGTGACT TGGCTCAGGA ATTGGATCAC 
GATGAATTCG ATGGACCACA TATGCTGAAT GGTGAACGTC CAGCGATTAT TGCTCCTCCG 
GAAAGCGAAT GGTACCATGG ACGACTCGAT CGCTACTCTG CAGAGTCTCG CCTACGCGGA 
AGCAGTAAAC TGGGCAGCTA TTTAGTTCGC GAAAGTGACC GCAAACCTGG CTCCTATGTT 
CTGAGTTATT ATGGCCGCAC CGGCATAAAT CATTTTCGGA TTACAGCTGT GTGCGGAGAC 
TTCTATATCG GAGGACGACA ATTCATTTCC TTAAGCGACT TGGTTGGTTA CTATACCTCA 
TGTAGTGATC TACTGAAACG CGAACGCTTG GCCATACCAG TTGCTCCGCC GGAGCCAGTT 
AATGATAAGA AACGCGTCGT AGCCATCCTG CCTTACACAA AAATGCCAGA GACCGATGAA 
CTGAGTTTCC AGAAAGGTGA TATCTTTTTT GTACACAATG ACATGGGCGA TGGCTGGTTG 
TGGGTGACTG CCCATCGTAC GGGCGAGCAG GGAATGATAT TCAGAGAACT TGTTGACGAT 
CTGGATGTAT CTATCGATCC AAATACGGTG TTTCCCTGGT TCCATCCCAA CTGCACCAAA 
AACGAAGCCG TCGATATGTT GGTTAAAGCT GGACCAGGAA GTTTCCTCGT TCGCCCAAGT 
GACAACTCAC CGGGGGATTA CTCTCTATTC TTTCATATAA ACAATCAAAT CCAACGCTTT 
CGTATTGAAA AGAAAGGCGT GCGCTATTTA ATGGGCGGTC GTACATTCGA ATGCTTGGAT 
GCGGTTATAA ATCGATATCG CAAAGAGCAA ATTGTGGAGG GCCACTCACT GAACCATCCA 
GTTGTCAATG GCATTCAGCC CGAGTTCAAT CAGCAATATG TTGTGGAAAA GGCCGCTGAA 
AAGATCTACG CAACATTGCG CGAGTGTCGT GACCAAATCG GTCTCAAAAA GATCAAGGGA 
ATCAAACATC ACGGCCATTT GAACAAGAAG TCGGACAAAA CGACGAAATG GAAGCAACTC 
TATTTCGCTT TAATCAACGA TGGTTCCGAG ACGCAACTAT GCTTCTACGA CAATCCGAAG 
AAGACCAAAC CGAAGGGTCT GATCGATTTG TCCTGTGCTT ACCTCTATCA ATGTCACGAT 
TCGCTGTGGG AGCGTCCCTA TTGCTTCCAA ATTGTCGAAA GGGCCCTGCC ATGCTTGGCC 
ACAGTGACGT ATCTGTGTGC TCCTAGTCAG GAAAGCTACG TGGAGTGGAT TAATTCGCTC 
AAGGCACAAT GCGATTCCCA GCTGAGTCGT GCCCAGAAGA AGGTATCACG CCTTCGTGAG 
CTTCGTTGTC TTAATCTGCA TGTGCTGGAA GCTCATCGAT TACCATTTAA ACTAGTGCCA 
CATCCATACT GCAGTATTTC CCTCAATCAA GTCAAAGTGG GCAAAACTCG TGTCAAAATT 
GCACCCGAAC CCGTTTGGGA AGAGGAGTTT GTGCTAGACG ATGTTCCGCC AGATGTTGTC 
TCCCTAACCA TAACATTAAT ATCGCGCGGC AAACGCGGTA AGGACTCCGA AGTGGCTGAG 
CTAACCATTG ACTTGTCTAG TCTAAAGAAT GGCCAGGAAA CCGAGGGCTG GTACCAACTC 
ACTGGCATGA CGCCGATGGG TGAATGGGGC TCGCTACGAT TGCGTATGCG CTACCTCGAC 
GATCTGATAA TGCCGTGTGA AGAGTACAGT CCTCTGCAGC AATTGCTCCT CGAATCGGAG 
CTGTATGCTG TGAAGGCGCT GGCTGAATTG TGCCATAATG ATCGGGTTCC TTTGGCCACG 
GCTCTGTTGC GAGTCTTTAG GCAGGAGAAA CGGGAAACGG AACTAATTCG AATGCTGTGC 
CAAGCGGAGG TGACGCGCGA AAACGAAACC ACAACGCTTT TCCGTGGCGC ATCCTTGGCC 
ACCACCCTAA TGGATCTATA TATGCGCACC GAATGCAGCG GCTTCCTGCA ATCGGCCGTC 
AGCGAAACCG TGCAGCGCAT TTTGGAGAGC AAGCAGTCGG CTGAATTGAA TCCCACGAAA 
ATGGACGTCA ACGATGATGC CTGTACGAAT GCCGAGTTTC TATTGCAAAT CCTCGATCTG 
GTCACCCAGT CGATATTCAC TTCACCGGAC GCCTGTCCAC GCAACGTGCG TTTCATTTGC 
AGCTGTCTTC AGAAGGCTGT GATGGCCAAA TGGCCGACAG AGCGGCTCGT TCGCACACGC 
GTCGTCTCTG GCTTTATATT CTTGCGCCTT CTGTGCCCAG CGCTGCTCAA TCCACGCCAA 
TTTGGATTGG TCAGCGAGAC GCCACCGATG GCAGCAACAC GATCTTTGGT CATGGTCGCC 
AAGTGTCTGC AGAATCTGGC CAACTTGATT GAATTCGGCG GCAAGGAACA ATACATGGAG 
GTTGTCAATC CGTTTATCTT GAAGAACAAG GAGCGTATGA TTGTTTTCTT GGATCAACTG 
TCCTCGGTGA ACGATCCGAA CCAACCGCTT GGAATGTTTG TCGAACAAAG TACAAATCTG 
AATTCACAAG ACACTGGACG CGAGCTGGCT ACCTTGCATC ACATCTGCGT ATCTCATTCG 
CAAGAACTGC ACGAGCTATC CAACATACTT TCAATTAAGA AGCTGGTCAC AGTGACGGAC 
ATGCTGACCA AACACAAGCT GAAGTATCGC GAGATGATCA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523361.1
CDS	353..3217
Translation	MADLIHLGNIDVVIQKDKPGSPTGCSDDGNLAGLGGMTGLGGIMAPGGIDLGDLAQELDHDEFDGPHMLNGERPAIIAPPESEWYHGRLDRYSAESRLRGSSKLGSYLVRESDRKPGSYVLSYYGRTGINHFRITAVCGDFYIGGRQFISLSDLVGYYTSCSDLLKRERLAIPVAPPEPVNDKKRVVAILPYTKMPETDELSFQKGDIFFVHNDMGDGWLWVTAHRTGEQGMIFRELVDDLDVSIDPNTVFPWFHPNCTKNEAVDMLVKAGPGSFLVRPSDNSPGDYSLFFHINNQIQRFRIEKKGVRYLMGGRTFECLDAVINRYRKEQIVEGHSLNHPVVNGIQPEFNQQYVVEKAAEKIYATLRECRDQIGLKKIKGIKHHGHLNKKSDKTTKWKQLYFALINDGSETQLCFYDNPKKTKPKGLIDLSCAYLYQCHDSLWERPYCFQIVERALPCLATVTYLCAPSQESYVEWINSLKAQCDSQLSRAQKKVSRLRELRCLNLHVLEAHRLPFKLVPHPYCSISLNQVKVGKTRVKIAPEPVWEEEFVLDDVPPDVVSLTITLISRGKRGKDSEVAELTIDLSSLKNGQETEGWYQLTGMTPMGEWGSLRLRMRYLDDLIMPCEEYSPLQQLLLESELYAVKALAELCHNDRVPLATALLRVFRQEKRETELIRMLCQAEVTRENETTTLFRGASLATTLMDLYMRTECSGFLQSAVSETVQRILESKQSAELNPTKMDVNDDACTNAEFLLQILDLVTQSIFTSPDACPRNVRFICSCLQKAVMAKWPTERLVRTRVVSGFIFLRLLCPALLNPRQFGLVSETPPMAATRSLVMVAKCLQNLANLIEFGGKEQYMEVVNPFILKNKERMIVFLDQLSSVNDPNQPLGMFVEQSTNLNSQDTGRELATLHHICVSHSQELHELSNILSIKKLVTVTDMLTKHKLKYREMIS

Target ORF information:

RefSeq Version	NM_078637.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar peduncle (vap), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078637.3

ORF Insert Sequence:

ATGGCGGACC TAATACATTT GGGAAACATT GATGTTGTCA TCCAGAAAGA TAAGCCCGGA 
TCGCCAACGG GCTGTTCGGA TGATGGGAAT CTTGCGGGGC TGGGTGGCAT GACCGGATTG 
GGTGGCATAA TGGCACCTGG CGGCATCGAC TTGGGTGACT TGGCTCAGGA ATTGGATCAC 
GATGAATTCG ATGGACCACA TATGCTGAAT GGTGAACGTC CAGCGATTAT TGCTCCTCCG 
GAAAGCGAAT GGTACCATGG ACGACTCGAT CGCTACTCTG CAGAGTCTCG CCTACGCGGA 
AGCAGTAAAC TGGGCAGCTA TTTAGTTCGC GAAAGTGACC GCAAACCTGG CTCCTATGTT 
CTGAGTTATT ATGGCCGCAC CGGCATAAAT CATTTTCGGA TTACAGCTGT GTGCGGAGAC 
TTCTATATCG GAGGACGACA ATTCATTTCC TTAAGCGACT TGGTTGGTTA CTATACCTCA 
TGTAGTGATC TACTGAAACG CGAACGCTTG GCCATACCAG TTGCTCCGCC GGAGCCAGTT 
AATGATAAGA AACGCGTCGT AGCCATCCTG CCTTACACAA AAATGCCAGA GACCGATGAA 
CTGAGTTTCC AGAAAGGTGA TATCTTTTTT GTACACAATG ACATGGGCGA TGGCTGGTTG 
TGGGTGACTG CCCATCGTAC GGGCGAGCAG GGAATGATAT TCAGAGAACT TGTTGACGAT 
CTGGATGTAT CTATCGATCC AAATACGGTG TTTCCCTGGT TCCATCCCAA CTGCACCAAA 
AACGAAGCCG TCGATATGTT GGTTAAAGCT GGACCAGGAA GTTTCCTCGT TCGCCCAAGT 
GACAACTCAC CGGGGGATTA CTCTCTATTC TTTCATATAA ACAATCAAAT CCAACGCTTT 
CGTATTGAAA AGAAAGGCGT GCGCTATTTA ATGGGCGGTC GTACATTCGA ATGCTTGGAT 
GCGGTTATAA ATCGATATCG CAAAGAGCAA ATTGTGGAGG GCCACTCACT GAACCATCCA 
GTTGTCAATG GCATTCAGCC CGAGTTCAAT CAGCAATATG TTGTGGAAAA GGCCGCTGAA 
AAGATCTACG CAACATTGCG CGAGTGTCGT GACCAAATCG GTCTCAAAAA GATCAAGGGA 
ATCAAACATC ACGGCCATTT GAACAAGAAG TCGGACAAAA CGACGAAATG GAAGCAACTC 
TATTTCGCTT TAATCAACGA TGGTTCCGAG ACGCAACTAT GCTTCTACGA CAATCCGAAG 
AAGACCAAAC CGAAGGGTCT GATCGATTTG TCCTGTGCTT ACCTCTATCA ATGTCACGAT 
TCGCTGTGGG AGCGTCCCTA TTGCTTCCAA ATTGTCGAAA GGGCCCTGCC ATGCTTGGCC 
ACAGTGACGT ATCTGTGTGC TCCTAGTCAG GAAAGCTACG TGGAGTGGAT TAATTCGCTC 
AAGGCACAAT GCGATTCCCA GCTGAGTCGT GCCCAGAAGA AGGTATCACG CCTTCGTGAG 
CTTCGTTGTC TTAATCTGCA TGTGCTGGAA GCTCATCGAT TACCATTTAA ACTAGTGCCA 
CATCCATACT GCAGTATTTC CCTCAATCAA GTCAAAGTGG GCAAAACTCG TGTCAAAATT 
GCACCCGAAC CCGTTTGGGA AGAGGAGTTT GTGCTAGACG ATGTTCCGCC AGATGTTGTC 
TCCCTAACCA TAACATTAAT ATCGCGCGGC AAACGCGGTA AGGACTCCGA AGTGGCTGAG 
CTAACCATTG ACTTGTCTAG TCTAAAGAAT GGCCAGGAAA CCGAGGGCTG GTACCAACTC 
ACTGGCATGA CGCCGATGGG TGAATGGGGC TCGCTACGAT TGCGTATGCG CTACCTCGAC 
GATCTGATAA TGCCGTGTGA AGAGTACAGT CCTCTGCAGC AATTGCTCCT CGAATCGGAG 
CTGTATGCTG TGAAGGCGCT GGCTGAATTG TGCCATAATG ATCGGGTTCC TTTGGCCACG 
GCTCTGTTGC GAGTCTTTAG GCAGGAGAAA CGGGAAACGG AACTAATTCG AATGCTGTGC 
CAAGCGGAGG TGACGCGCGA AAACGAAACC ACAACGCTTT TCCGTGGCGC ATCCTTGGCC 
ACCACCCTAA TGGATCTATA TATGCGCACC GAATGCAGCG GCTTCCTGCA ATCGGCCGTC 
AGCGAAACCG TGCAGCGCAT TTTGGAGAGC AAGCAGTCGG CTGAATTGAA TCCCACGAAA 
ATGGACGTCA ACGATGATGC CTGTACGAAT GCCGAGTTTC TATTGCAAAT CCTCGATCTG 
GTCACCCAGT CGATATTCAC TTCACCGGAC GCCTGTCCAC GCAACGTGCG TTTCATTTGC 
AGCTGTCTTC AGAAGGCTGT GATGGCCAAA TGGCCGACAG AGCGGCTCGT TCGCACACGC 
GTCGTCTCTG GCTTTATATT CTTGCGCCTT CTGTGCCCAG CGCTGCTCAA TCCACGCCAA 
TTTGGATTGG TCAGCGAGAC GCCACCGATG GCAGCAACAC GATCTTTGGT CATGGTCGCC 
AAGTGTCTGC AGAATCTGGC CAACTTGATT GAATTCGGCG GCAAGGAACA ATACATGGAG 
GTTGTCAATC CGTTTATCTT GAAGAACAAG GAGCGTATGA TTGTTTTCTT GGATCAACTG 
TCCTCGGTGA ACGATCCGAA CCAACCGCTT GGAATGTTTG TCGAACAAAG TACAAATCTG 
AATTCACAAG ACACTGGACG CGAGCTGGCT ACCTTGCATC ACATCTGCGT ATCTCATTCG 
CAAGAACTGC ACGAGCTATC CAACATACTT TCAATTAAGA AGCTGGTCAC AGTGACGGAC 
ATGCTGACCA AACACAAGCT GAAGTATCGC GAGATGATCA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vap ( NM_167480.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727925.1 Drosophila melanogaster vacuolar peduncle (vap), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa01749
Clone ID Related Accession (Same CDS sequence)	NM_001298386.1 , NM_167481.3 , NM_001298385.1 , NM_206759.2 , NM_078637.3 , NM_167480.2
Accession Version	NM_167480.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2865bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar peduncle, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167480.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGACC TAATACATTT GGGAAACATT GATGTTGTCA TCCAGAAAGA TAAGCCCGGA 
TCGCCAACGG GCTGTTCGGA TGATGGGAAT CTTGCGGGGC TGGGTGGCAT GACCGGATTG 
GGTGGCATAA TGGCACCTGG CGGCATCGAC TTGGGTGACT TGGCTCAGGA ATTGGATCAC 
GATGAATTCG ATGGACCACA TATGCTGAAT GGTGAACGTC CAGCGATTAT TGCTCCTCCG 
GAAAGCGAAT GGTACCATGG ACGACTCGAT CGCTACTCTG CAGAGTCTCG CCTACGCGGA 
AGCAGTAAAC TGGGCAGCTA TTTAGTTCGC GAAAGTGACC GCAAACCTGG CTCCTATGTT 
CTGAGTTATT ATGGCCGCAC CGGCATAAAT CATTTTCGGA TTACAGCTGT GTGCGGAGAC 
TTCTATATCG GAGGACGACA ATTCATTTCC TTAAGCGACT TGGTTGGTTA CTATACCTCA 
TGTAGTGATC TACTGAAACG CGAACGCTTG GCCATACCAG TTGCTCCGCC GGAGCCAGTT 
AATGATAAGA AACGCGTCGT AGCCATCCTG CCTTACACAA AAATGCCAGA GACCGATGAA 
CTGAGTTTCC AGAAAGGTGA TATCTTTTTT GTACACAATG ACATGGGCGA TGGCTGGTTG 
TGGGTGACTG CCCATCGTAC GGGCGAGCAG GGAATGATAT TCAGAGAACT TGTTGACGAT 
CTGGATGTAT CTATCGATCC AAATACGGTG TTTCCCTGGT TCCATCCCAA CTGCACCAAA 
AACGAAGCCG TCGATATGTT GGTTAAAGCT GGACCAGGAA GTTTCCTCGT TCGCCCAAGT 
GACAACTCAC CGGGGGATTA CTCTCTATTC TTTCATATAA ACAATCAAAT CCAACGCTTT 
CGTATTGAAA AGAAAGGCGT GCGCTATTTA ATGGGCGGTC GTACATTCGA ATGCTTGGAT 
GCGGTTATAA ATCGATATCG CAAAGAGCAA ATTGTGGAGG GCCACTCACT GAACCATCCA 
GTTGTCAATG GCATTCAGCC CGAGTTCAAT CAGCAATATG TTGTGGAAAA GGCCGCTGAA 
AAGATCTACG CAACATTGCG CGAGTGTCGT GACCAAATCG GTCTCAAAAA GATCAAGGGA 
ATCAAACATC ACGGCCATTT GAACAAGAAG TCGGACAAAA CGACGAAATG GAAGCAACTC 
TATTTCGCTT TAATCAACGA TGGTTCCGAG ACGCAACTAT GCTTCTACGA CAATCCGAAG 
AAGACCAAAC CGAAGGGTCT GATCGATTTG TCCTGTGCTT ACCTCTATCA ATGTCACGAT 
TCGCTGTGGG AGCGTCCCTA TTGCTTCCAA ATTGTCGAAA GGGCCCTGCC ATGCTTGGCC 
ACAGTGACGT ATCTGTGTGC TCCTAGTCAG GAAAGCTACG TGGAGTGGAT TAATTCGCTC 
AAGGCACAAT GCGATTCCCA GCTGAGTCGT GCCCAGAAGA AGGTATCACG CCTTCGTGAG 
CTTCGTTGTC TTAATCTGCA TGTGCTGGAA GCTCATCGAT TACCATTTAA ACTAGTGCCA 
CATCCATACT GCAGTATTTC CCTCAATCAA GTCAAAGTGG GCAAAACTCG TGTCAAAATT 
GCACCCGAAC CCGTTTGGGA AGAGGAGTTT GTGCTAGACG ATGTTCCGCC AGATGTTGTC 
TCCCTAACCA TAACATTAAT ATCGCGCGGC AAACGCGGTA AGGACTCCGA AGTGGCTGAG 
CTAACCATTG ACTTGTCTAG TCTAAAGAAT GGCCAGGAAA CCGAGGGCTG GTACCAACTC 
ACTGGCATGA CGCCGATGGG TGAATGGGGC TCGCTACGAT TGCGTATGCG CTACCTCGAC 
GATCTGATAA TGCCGTGTGA AGAGTACAGT CCTCTGCAGC AATTGCTCCT CGAATCGGAG 
CTGTATGCTG TGAAGGCGCT GGCTGAATTG TGCCATAATG ATCGGGTTCC TTTGGCCACG 
GCTCTGTTGC GAGTCTTTAG GCAGGAGAAA CGGGAAACGG AACTAATTCG AATGCTGTGC 
CAAGCGGAGG TGACGCGCGA AAACGAAACC ACAACGCTTT TCCGTGGCGC ATCCTTGGCC 
ACCACCCTAA TGGATCTATA TATGCGCACC GAATGCAGCG GCTTCCTGCA ATCGGCCGTC 
AGCGAAACCG TGCAGCGCAT TTTGGAGAGC AAGCAGTCGG CTGAATTGAA TCCCACGAAA 
ATGGACGTCA ACGATGATGC CTGTACGAAT GCCGAGTTTC TATTGCAAAT CCTCGATCTG 
GTCACCCAGT CGATATTCAC TTCACCGGAC GCCTGTCCAC GCAACGTGCG TTTCATTTGC 
AGCTGTCTTC AGAAGGCTGT GATGGCCAAA TGGCCGACAG AGCGGCTCGT TCGCACACGC 
GTCGTCTCTG GCTTTATATT CTTGCGCCTT CTGTGCCCAG CGCTGCTCAA TCCACGCCAA 
TTTGGATTGG TCAGCGAGAC GCCACCGATG GCAGCAACAC GATCTTTGGT CATGGTCGCC 
AAGTGTCTGC AGAATCTGGC CAACTTGATT GAATTCGGCG GCAAGGAACA ATACATGGAG 
GTTGTCAATC CGTTTATCTT GAAGAACAAG GAGCGTATGA TTGTTTTCTT GGATCAACTG 
TCCTCGGTGA ACGATCCGAA CCAACCGCTT GGAATGTTTG TCGAACAAAG TACAAATCTG 
AATTCACAAG ACACTGGACG CGAGCTGGCT ACCTTGCATC ACATCTGCGT ATCTCATTCG 
CAAGAACTGC ACGAGCTATC CAACATACTT TCAATTAAGA AGCTGGTCAC AGTGACGGAC 
ATGCTGACCA AACACAAGCT GAAGTATCGC GAGATGATCA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727925.1
CDS	470..3334
Translation	MADLIHLGNIDVVIQKDKPGSPTGCSDDGNLAGLGGMTGLGGIMAPGGIDLGDLAQELDHDEFDGPHMLNGERPAIIAPPESEWYHGRLDRYSAESRLRGSSKLGSYLVRESDRKPGSYVLSYYGRTGINHFRITAVCGDFYIGGRQFISLSDLVGYYTSCSDLLKRERLAIPVAPPEPVNDKKRVVAILPYTKMPETDELSFQKGDIFFVHNDMGDGWLWVTAHRTGEQGMIFRELVDDLDVSIDPNTVFPWFHPNCTKNEAVDMLVKAGPGSFLVRPSDNSPGDYSLFFHINNQIQRFRIEKKGVRYLMGGRTFECLDAVINRYRKEQIVEGHSLNHPVVNGIQPEFNQQYVVEKAAEKIYATLRECRDQIGLKKIKGIKHHGHLNKKSDKTTKWKQLYFALINDGSETQLCFYDNPKKTKPKGLIDLSCAYLYQCHDSLWERPYCFQIVERALPCLATVTYLCAPSQESYVEWINSLKAQCDSQLSRAQKKVSRLRELRCLNLHVLEAHRLPFKLVPHPYCSISLNQVKVGKTRVKIAPEPVWEEEFVLDDVPPDVVSLTITLISRGKRGKDSEVAELTIDLSSLKNGQETEGWYQLTGMTPMGEWGSLRLRMRYLDDLIMPCEEYSPLQQLLLESELYAVKALAELCHNDRVPLATALLRVFRQEKRETELIRMLCQAEVTRENETTTLFRGASLATTLMDLYMRTECSGFLQSAVSETVQRILESKQSAELNPTKMDVNDDACTNAEFLLQILDLVTQSIFTSPDACPRNVRFICSCLQKAVMAKWPTERLVRTRVVSGFIFLRLLCPALLNPRQFGLVSETPPMAATRSLVMVAKCLQNLANLIEFGGKEQYMEVVNPFILKNKERMIVFLDQLSSVNDPNQPLGMFVEQSTNLNSQDTGRELATLHHICVSHSQELHELSNILSIKKLVTVTDMLTKHKLKYREMIS

Target ORF information:

RefSeq Version	NM_167480.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar peduncle (vap), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167480.2

ORF Insert Sequence:

ATGGCGGACC TAATACATTT GGGAAACATT GATGTTGTCA TCCAGAAAGA TAAGCCCGGA 
TCGCCAACGG GCTGTTCGGA TGATGGGAAT CTTGCGGGGC TGGGTGGCAT GACCGGATTG 
GGTGGCATAA TGGCACCTGG CGGCATCGAC TTGGGTGACT TGGCTCAGGA ATTGGATCAC 
GATGAATTCG ATGGACCACA TATGCTGAAT GGTGAACGTC CAGCGATTAT TGCTCCTCCG 
GAAAGCGAAT GGTACCATGG ACGACTCGAT CGCTACTCTG CAGAGTCTCG CCTACGCGGA 
AGCAGTAAAC TGGGCAGCTA TTTAGTTCGC GAAAGTGACC GCAAACCTGG CTCCTATGTT 
CTGAGTTATT ATGGCCGCAC CGGCATAAAT CATTTTCGGA TTACAGCTGT GTGCGGAGAC 
TTCTATATCG GAGGACGACA ATTCATTTCC TTAAGCGACT TGGTTGGTTA CTATACCTCA 
TGTAGTGATC TACTGAAACG CGAACGCTTG GCCATACCAG TTGCTCCGCC GGAGCCAGTT 
AATGATAAGA AACGCGTCGT AGCCATCCTG CCTTACACAA AAATGCCAGA GACCGATGAA 
CTGAGTTTCC AGAAAGGTGA TATCTTTTTT GTACACAATG ACATGGGCGA TGGCTGGTTG 
TGGGTGACTG CCCATCGTAC GGGCGAGCAG GGAATGATAT TCAGAGAACT TGTTGACGAT 
CTGGATGTAT CTATCGATCC AAATACGGTG TTTCCCTGGT TCCATCCCAA CTGCACCAAA 
AACGAAGCCG TCGATATGTT GGTTAAAGCT GGACCAGGAA GTTTCCTCGT TCGCCCAAGT 
GACAACTCAC CGGGGGATTA CTCTCTATTC TTTCATATAA ACAATCAAAT CCAACGCTTT 
CGTATTGAAA AGAAAGGCGT GCGCTATTTA ATGGGCGGTC GTACATTCGA ATGCTTGGAT 
GCGGTTATAA ATCGATATCG CAAAGAGCAA ATTGTGGAGG GCCACTCACT GAACCATCCA 
GTTGTCAATG GCATTCAGCC CGAGTTCAAT CAGCAATATG TTGTGGAAAA GGCCGCTGAA 
AAGATCTACG CAACATTGCG CGAGTGTCGT GACCAAATCG GTCTCAAAAA GATCAAGGGA 
ATCAAACATC ACGGCCATTT GAACAAGAAG TCGGACAAAA CGACGAAATG GAAGCAACTC 
TATTTCGCTT TAATCAACGA TGGTTCCGAG ACGCAACTAT GCTTCTACGA CAATCCGAAG 
AAGACCAAAC CGAAGGGTCT GATCGATTTG TCCTGTGCTT ACCTCTATCA ATGTCACGAT 
TCGCTGTGGG AGCGTCCCTA TTGCTTCCAA ATTGTCGAAA GGGCCCTGCC ATGCTTGGCC 
ACAGTGACGT ATCTGTGTGC TCCTAGTCAG GAAAGCTACG TGGAGTGGAT TAATTCGCTC 
AAGGCACAAT GCGATTCCCA GCTGAGTCGT GCCCAGAAGA AGGTATCACG CCTTCGTGAG 
CTTCGTTGTC TTAATCTGCA TGTGCTGGAA GCTCATCGAT TACCATTTAA ACTAGTGCCA 
CATCCATACT GCAGTATTTC CCTCAATCAA GTCAAAGTGG GCAAAACTCG TGTCAAAATT 
GCACCCGAAC CCGTTTGGGA AGAGGAGTTT GTGCTAGACG ATGTTCCGCC AGATGTTGTC 
TCCCTAACCA TAACATTAAT ATCGCGCGGC AAACGCGGTA AGGACTCCGA AGTGGCTGAG 
CTAACCATTG ACTTGTCTAG TCTAAAGAAT GGCCAGGAAA CCGAGGGCTG GTACCAACTC 
ACTGGCATGA CGCCGATGGG TGAATGGGGC TCGCTACGAT TGCGTATGCG CTACCTCGAC 
GATCTGATAA TGCCGTGTGA AGAGTACAGT CCTCTGCAGC AATTGCTCCT CGAATCGGAG 
CTGTATGCTG TGAAGGCGCT GGCTGAATTG TGCCATAATG ATCGGGTTCC TTTGGCCACG 
GCTCTGTTGC GAGTCTTTAG GCAGGAGAAA CGGGAAACGG AACTAATTCG AATGCTGTGC 
CAAGCGGAGG TGACGCGCGA AAACGAAACC ACAACGCTTT TCCGTGGCGC ATCCTTGGCC 
ACCACCCTAA TGGATCTATA TATGCGCACC GAATGCAGCG GCTTCCTGCA ATCGGCCGTC 
AGCGAAACCG TGCAGCGCAT TTTGGAGAGC AAGCAGTCGG CTGAATTGAA TCCCACGAAA 
ATGGACGTCA ACGATGATGC CTGTACGAAT GCCGAGTTTC TATTGCAAAT CCTCGATCTG 
GTCACCCAGT CGATATTCAC TTCACCGGAC GCCTGTCCAC GCAACGTGCG TTTCATTTGC 
AGCTGTCTTC AGAAGGCTGT GATGGCCAAA TGGCCGACAG AGCGGCTCGT TCGCACACGC 
GTCGTCTCTG GCTTTATATT CTTGCGCCTT CTGTGCCCAG CGCTGCTCAA TCCACGCCAA 
TTTGGATTGG TCAGCGAGAC GCCACCGATG GCAGCAACAC GATCTTTGGT CATGGTCGCC 
AAGTGTCTGC AGAATCTGGC CAACTTGATT GAATTCGGCG GCAAGGAACA ATACATGGAG 
GTTGTCAATC CGTTTATCTT GAAGAACAAG GAGCGTATGA TTGTTTTCTT GGATCAACTG 
TCCTCGGTGA ACGATCCGAA CCAACCGCTT GGAATGTTTG TCGAACAAAG TACAAATCTG 
AATTCACAAG ACACTGGACG CGAGCTGGCT ACCTTGCATC ACATCTGCGT ATCTCATTCG 
CAAGAACTGC ACGAGCTATC CAACATACTT TCAATTAAGA AGCTGGTCAC AGTGACGGAC 
ATGCTGACCA AACACAAGCT GAAGTATCGC GAGATGATCA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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vap cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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