exd cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	exd
Entrez Gene ID	32567
Full Name	extradenticle
Synonyms	CG8933,DExd,Dm-EXD,Dmel\CG8933,Dpbx,EXD,Exd,Pbx1,anon-EST:fe1H3,l(1)IV,lincRNA.S9404,td48
General protein information	Preferred Names extradenticle Names CG8933 gene product from transcript CG8933-RA CG8933-PA CG8933-PB CG8933-PD CG8933-PE Pbx1-oncogene-like anon-fast-evolving-1H3 exd-PA exd-PB exd-PD exd-PE
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 14A5-14A5

mRNA and Protein(s)

mRNA	Protein	Name
NM_078636.5	NP_523360.1	extradenticle, isoform A
NM_167478.3	NP_727923.1	extradenticle, isoform B
NM_001272663.2	NP_001259592.1	extradenticle, isoform D
NM_001272664.1	NP_001259593.1	extradenticle, isoform E

Pathways from BioSystems

Homologies

Bos taurus (cattle)	PBX1	NP_001179697.1
Gallus gallus (chicken)	PBX1	NP_990077.1
Danio rerio (zebrafish)	pbx1b	NP_001077322.1
Drosophila melanogaster (fruit fly)	exd	NP_523360.1
Anopheles gambiae (African malaria mosquito)	EXD_ANOGA	XP_318146.2
Caenorhabditis elegans (roundworm)	ceh-20	NP_001022555.1
Pan troglodytes (chimpanzee)	PBX1	XP_001174529.1
Canis lupus familiaris (dog)	PBX1	XP_545786.2
Mus musculus (house mouse)	Pbx1	NP_899198.1
Danio rerio (zebrafish)	pbx1a	NP_571689.1
Homo sapiens (human)	PBX1	NP_002576.1
Rattus norvegicus (Norway rat)	Pbx1	NP_001128334.1
Xenopus tropicalis (tropical clawed frog)	pbx1	NP_001135549.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Grh, AbdA, and Exd function together at multiple motifs on the apoptotic enhancer. Exd-Grh-Notch work with different Hox genes through region specific enhancers to pattern respective segments of larval central nervous system.
Title: Combinatorial action of Grainyhead, Extradenticle and Notch in regulating Hox mediated apoptosis in Drosophila larval CNS.

Hox protein Ultrabithorax (Ubx) in complex with its cofactor Extradenticle (Exd) bound specifically to clusters of very low affinity sites in enhancers of the shavenbaby gene of Drosophila. These low affinity sites conferred specificity for Ubx binding in vivo, but multiple clustered sites were required for robust expression when embryos developed in variable environments.
Title: Low affinity binding site clusters confer hox specificity and regulatory robustness.

Hth functions together with its co-factor Extradenticle to repress the R8-specific factor Senseless in dorsal rim area R8 cells, allowing expression of an ultraviolet-sensitive R7 Rhodopsin.
Title: Homothorax and Extradenticle alter the transcription factor network in Drosophila ommatidia at the dorsal rim of the retina.

In the flight muscles, exd and hth are genetically upstream of another muscle identity gene, salm, and are direct transcriptional regulators of the signature flight muscle structural gene, Actin88F.
Title: Extradenticle and homothorax control adult muscle fiber identity in Drosophila.

cooperative binding sites for En, with the homeodomain-containing Hox cofactors Extradenticle (Exd) and Homothorax (Hth) was localized, within two CRMs that drive similar expression patterns
Title: Engrailed cooperates directly with Extradenticle and Homothorax on a distinct class of homeodomain binding sites to repress sloppy paired.

The following exd gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the exd cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01747	NM_078636.5 Latest version!	Drosophila melanogaster extradenticle (exd), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa01747	NM_001272664.1 Latest version!	Drosophila melanogaster extradenticle (exd), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa01747	NM_167478.3 Latest version!	Drosophila melanogaster extradenticle (exd), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa01747	NM_001272663.2 Latest version!	Drosophila melanogaster extradenticle (exd), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

exd ( NM_078636.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523360.1 Drosophila melanogaster extradenticle (exd), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa01747
Clone ID Related Accession (Same CDS sequence)	NM_078636.5 , NM_167478.3 , NM_001272663.2 , NM_001272664.1
Accession Version	NM_078636.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	extradenticle, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_078636.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGACC CCAATCGCAT GTTGGCCCAC ACCGGCGGCA TGATGGCTCC GCAAGGATAC 
GGCTTGTCTG GCCAGGATGA TGGCCAGAAT GCCGGCAGCG AGAACGAGGT GCGCAAGCAG 
AAGGACATCG GCGAGATATT GCAACAGATA ATGAGTATCT CGGAGCAATC ACTTGACGAG 
GCCCAGGCCA GAAAACATAC ACTAAACTGT CACCGAATGA AGCCGGCACT TTTCTCTGTA 
CTTTGCGAAA TCAAGGAGAA GACCGTCCTC TCGATTCGCA ACACCCAGGA GGAGGAGCCC 
CCAGATCCGC AACTGATGCG CTTGGACAAC ATGCTGATTG CCGAGGGCGT GGCCGGACCC 
GAGAAGGGCG GTGGCGGAGC AGCGGCTGCC TCTGCGGCTG CGGCCAGCCA GGGCGGTTCC 
CTGTCCATCG ATGGTGCTGA CAATGCCATT GAGCATTCCG ACTACCGTGC CAAGCTGGCA 
CAGATCCGTC AAATATATCA CCAGGAATTG GAGAAGTACG AGCAGGCATG CAACGAGTTC 
ACCACGCATG TCATGAATCT TCTACGCGAA CAGAGTCGCA CGAGACCCAT TACACCAAAG 
GAAATCGAGC GAATGGTGCA GATCATCCAC AAAAAGTTTA GTTCCATACA AATGCAGCTG 
AAGCAGTCGA CCTGCGAGGC TGTAATGATA CTTCGTTCGC GTTTCCTGGA CGCACGCCGC 
AAACGCCGCA ATTTCAGCAA GCAGGCATCC GAGATTCTCA ACGAGTACTT CTACAGTCAC 
TTGAGCAACC CATATCCATC CGAAGAGGCC AAAGAGGAGT TGGCACGAAA GTGTGGCATC 
ACGGTCTCGC AAGTATCGAA TTGGTTTGGC AACAAGCGTA TTCGCTATAA GAAGAACATT 
GGTAAGGCAC AGGAGGAGGC CAATTTGTAT GCGGCCAAGA AGGCGGCTGG TGCTTCACCG 
TATTCCATGG CCGGTCCACC CAGCGGGACA ACCACACCCA TGATGTCACC CGCTCCGCCA 
CAGGATTCCA TGGGCTATCC AATGGGCTCC GGCGGATACG ACCAGCAGCA ACCGTACGAC 
AACAGCATGG GCGGCTACGA CCCAAATCTC CATCAGGATC TAAGCCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523360.1
CDS	232..1362
Translation	MEDPNRMLAHTGGMMAPQGYGLSGQDDGQNAGSENEVRKQKDIGEILQQIMSISEQSLDEAQARKHTLNCHRMKPALFSVLCEIKEKTVLSIRNTQEEEPPDPQLMRLDNMLIAEGVAGPEKGGGGAAAASAAAASQGGSLSIDGADNAIEHSDYRAKLAQIRQIYHQELEKYEQACNEFTTHVMNLLREQSRTRPITPKEIERMVQIIHKKFSSIQMQLKQSTCEAVMILRSRFLDARRKRRNFSKQASEILNEYFYSHLSNPYPSEEAKEELARKCGITVSQVSNWFGNKRIRYKKNIGKAQEEANLYAAKKAAGASPYSMAGPPSGTTTPMMSPAPPQDSMGYPMGSGGYDQQQPYDNSMGGYDPNLHQDLSP

Target ORF information:

RefSeq Version	NM_078636.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster extradenticle (exd), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078636.5

ORF Insert Sequence:

ATGGAGGACC CCAATCGCAT GTTGGCCCAC ACCGGCGGCA TGATGGCTCC GCAAGGATAC 
GGCTTGTCTG GCCAGGATGA TGGCCAGAAT GCCGGCAGCG AGAACGAGGT GCGCAAGCAG 
AAGGACATCG GCGAGATATT GCAACAGATA ATGAGTATCT CGGAGCAATC ACTTGACGAG 
GCCCAGGCCA GAAAACATAC ACTAAACTGT CACCGAATGA AGCCGGCACT TTTCTCTGTA 
CTTTGCGAAA TCAAGGAGAA GACCGTCCTC TCGATTCGCA ACACCCAGGA GGAGGAGCCC 
CCAGATCCGC AACTGATGCG CTTGGACAAC ATGCTGATTG CCGAGGGCGT GGCCGGACCC 
GAGAAGGGCG GTGGCGGAGC AGCGGCTGCC TCTGCGGCTG CGGCCAGCCA GGGCGGTTCC 
CTGTCCATCG ATGGTGCTGA CAATGCCATT GAGCATTCCG ACTACCGTGC CAAGCTGGCA 
CAGATCCGTC AAATATATCA CCAGGAATTG GAGAAGTACG AGCAGGCATG CAACGAGTTC 
ACCACGCATG TCATGAATCT TCTACGCGAA CAGAGTCGCA CGAGACCCAT TACACCAAAG 
GAAATCGAGC GAATGGTGCA GATCATCCAC AAAAAGTTTA GTTCCATACA AATGCAGCTG 
AAGCAGTCGA CCTGCGAGGC TGTAATGATA CTTCGTTCGC GTTTCCTGGA CGCACGCCGC 
AAACGCCGCA ATTTCAGCAA GCAGGCATCC GAGATTCTCA ACGAGTACTT CTACAGTCAC 
TTGAGCAACC CATATCCATC CGAAGAGGCC AAAGAGGAGT TGGCACGAAA GTGTGGCATC 
ACGGTCTCGC AAGTATCGAA TTGGTTTGGC AACAAGCGTA TTCGCTATAA GAAGAACATT 
GGTAAGGCAC AGGAGGAGGC CAATTTGTAT GCGGCCAAGA AGGCGGCTGG TGCTTCACCG 
TATTCCATGG CCGGTCCACC CAGCGGGACA ACCACACCCA TGATGTCACC CGCTCCGCCA 
CAGGATTCCA TGGGCTATCC AATGGGCTCC GGCGGATACG ACCAGCAGCA ACCGTACGAC 
AACAGCATGG GCGGCTACGA CCCAAATCTC CATCAGGATC TAAGCCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

exd ( NM_167478.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727923.1 Drosophila melanogaster extradenticle (exd), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa01747
Clone ID Related Accession (Same CDS sequence)	NM_078636.5 , NM_167478.3 , NM_001272663.2 , NM_001272664.1
Accession Version	NM_167478.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	extradenticle, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167478.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGACC CCAATCGCAT GTTGGCCCAC ACCGGCGGCA TGATGGCTCC GCAAGGATAC 
GGCTTGTCTG GCCAGGATGA TGGCCAGAAT GCCGGCAGCG AGAACGAGGT GCGCAAGCAG 
AAGGACATCG GCGAGATATT GCAACAGATA ATGAGTATCT CGGAGCAATC ACTTGACGAG 
GCCCAGGCCA GAAAACATAC ACTAAACTGT CACCGAATGA AGCCGGCACT TTTCTCTGTA 
CTTTGCGAAA TCAAGGAGAA GACCGTCCTC TCGATTCGCA ACACCCAGGA GGAGGAGCCC 
CCAGATCCGC AACTGATGCG CTTGGACAAC ATGCTGATTG CCGAGGGCGT GGCCGGACCC 
GAGAAGGGCG GTGGCGGAGC AGCGGCTGCC TCTGCGGCTG CGGCCAGCCA GGGCGGTTCC 
CTGTCCATCG ATGGTGCTGA CAATGCCATT GAGCATTCCG ACTACCGTGC CAAGCTGGCA 
CAGATCCGTC AAATATATCA CCAGGAATTG GAGAAGTACG AGCAGGCATG CAACGAGTTC 
ACCACGCATG TCATGAATCT TCTACGCGAA CAGAGTCGCA CGAGACCCAT TACACCAAAG 
GAAATCGAGC GAATGGTGCA GATCATCCAC AAAAAGTTTA GTTCCATACA AATGCAGCTG 
AAGCAGTCGA CCTGCGAGGC TGTAATGATA CTTCGTTCGC GTTTCCTGGA CGCACGCCGC 
AAACGCCGCA ATTTCAGCAA GCAGGCATCC GAGATTCTCA ACGAGTACTT CTACAGTCAC 
TTGAGCAACC CATATCCATC CGAAGAGGCC AAAGAGGAGT TGGCACGAAA GTGTGGCATC 
ACGGTCTCGC AAGTATCGAA TTGGTTTGGC AACAAGCGTA TTCGCTATAA GAAGAACATT 
GGTAAGGCAC AGGAGGAGGC CAATTTGTAT GCGGCCAAGA AGGCGGCTGG TGCTTCACCG 
TATTCCATGG CCGGTCCACC CAGCGGGACA ACCACACCCA TGATGTCACC CGCTCCGCCA 
CAGGATTCCA TGGGCTATCC AATGGGCTCC GGCGGATACG ACCAGCAGCA ACCGTACGAC 
AACAGCATGG GCGGCTACGA CCCAAATCTC CATCAGGATC TAAGCCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727923.1
CDS	193..1323
Translation	MEDPNRMLAHTGGMMAPQGYGLSGQDDGQNAGSENEVRKQKDIGEILQQIMSISEQSLDEAQARKHTLNCHRMKPALFSVLCEIKEKTVLSIRNTQEEEPPDPQLMRLDNMLIAEGVAGPEKGGGGAAAASAAAASQGGSLSIDGADNAIEHSDYRAKLAQIRQIYHQELEKYEQACNEFTTHVMNLLREQSRTRPITPKEIERMVQIIHKKFSSIQMQLKQSTCEAVMILRSRFLDARRKRRNFSKQASEILNEYFYSHLSNPYPSEEAKEELARKCGITVSQVSNWFGNKRIRYKKNIGKAQEEANLYAAKKAAGASPYSMAGPPSGTTTPMMSPAPPQDSMGYPMGSGGYDQQQPYDNSMGGYDPNLHQDLSP

Target ORF information:

RefSeq Version	NM_167478.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster extradenticle (exd), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167478.3

ORF Insert Sequence:

ATGGAGGACC CCAATCGCAT GTTGGCCCAC ACCGGCGGCA TGATGGCTCC GCAAGGATAC 
GGCTTGTCTG GCCAGGATGA TGGCCAGAAT GCCGGCAGCG AGAACGAGGT GCGCAAGCAG 
AAGGACATCG GCGAGATATT GCAACAGATA ATGAGTATCT CGGAGCAATC ACTTGACGAG 
GCCCAGGCCA GAAAACATAC ACTAAACTGT CACCGAATGA AGCCGGCACT TTTCTCTGTA 
CTTTGCGAAA TCAAGGAGAA GACCGTCCTC TCGATTCGCA ACACCCAGGA GGAGGAGCCC 
CCAGATCCGC AACTGATGCG CTTGGACAAC ATGCTGATTG CCGAGGGCGT GGCCGGACCC 
GAGAAGGGCG GTGGCGGAGC AGCGGCTGCC TCTGCGGCTG CGGCCAGCCA GGGCGGTTCC 
CTGTCCATCG ATGGTGCTGA CAATGCCATT GAGCATTCCG ACTACCGTGC CAAGCTGGCA 
CAGATCCGTC AAATATATCA CCAGGAATTG GAGAAGTACG AGCAGGCATG CAACGAGTTC 
ACCACGCATG TCATGAATCT TCTACGCGAA CAGAGTCGCA CGAGACCCAT TACACCAAAG 
GAAATCGAGC GAATGGTGCA GATCATCCAC AAAAAGTTTA GTTCCATACA AATGCAGCTG 
AAGCAGTCGA CCTGCGAGGC TGTAATGATA CTTCGTTCGC GTTTCCTGGA CGCACGCCGC 
AAACGCCGCA ATTTCAGCAA GCAGGCATCC GAGATTCTCA ACGAGTACTT CTACAGTCAC 
TTGAGCAACC CATATCCATC CGAAGAGGCC AAAGAGGAGT TGGCACGAAA GTGTGGCATC 
ACGGTCTCGC AAGTATCGAA TTGGTTTGGC AACAAGCGTA TTCGCTATAA GAAGAACATT 
GGTAAGGCAC AGGAGGAGGC CAATTTGTAT GCGGCCAAGA AGGCGGCTGG TGCTTCACCG 
TATTCCATGG CCGGTCCACC CAGCGGGACA ACCACACCCA TGATGTCACC CGCTCCGCCA 
CAGGATTCCA TGGGCTATCC AATGGGCTCC GGCGGATACG ACCAGCAGCA ACCGTACGAC 
AACAGCATGG GCGGCTACGA CCCAAATCTC CATCAGGATC TAAGCCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

exd ( NM_001272663.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259592.1 Drosophila melanogaster extradenticle (exd), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa01747
Clone ID Related Accession (Same CDS sequence)	NM_078636.5 , NM_167478.3 , NM_001272663.2 , NM_001272664.1
Accession Version	NM_001272663.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	extradenticle, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001272663.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGACC CCAATCGCAT GTTGGCCCAC ACCGGCGGCA TGATGGCTCC GCAAGGATAC 
GGCTTGTCTG GCCAGGATGA TGGCCAGAAT GCCGGCAGCG AGAACGAGGT GCGCAAGCAG 
AAGGACATCG GCGAGATATT GCAACAGATA ATGAGTATCT CGGAGCAATC ACTTGACGAG 
GCCCAGGCCA GAAAACATAC ACTAAACTGT CACCGAATGA AGCCGGCACT TTTCTCTGTA 
CTTTGCGAAA TCAAGGAGAA GACCGTCCTC TCGATTCGCA ACACCCAGGA GGAGGAGCCC 
CCAGATCCGC AACTGATGCG CTTGGACAAC ATGCTGATTG CCGAGGGCGT GGCCGGACCC 
GAGAAGGGCG GTGGCGGAGC AGCGGCTGCC TCTGCGGCTG CGGCCAGCCA GGGCGGTTCC 
CTGTCCATCG ATGGTGCTGA CAATGCCATT GAGCATTCCG ACTACCGTGC CAAGCTGGCA 
CAGATCCGTC AAATATATCA CCAGGAATTG GAGAAGTACG AGCAGGCATG CAACGAGTTC 
ACCACGCATG TCATGAATCT TCTACGCGAA CAGAGTCGCA CGAGACCCAT TACACCAAAG 
GAAATCGAGC GAATGGTGCA GATCATCCAC AAAAAGTTTA GTTCCATACA AATGCAGCTG 
AAGCAGTCGA CCTGCGAGGC TGTAATGATA CTTCGTTCGC GTTTCCTGGA CGCACGCCGC 
AAACGCCGCA ATTTCAGCAA GCAGGCATCC GAGATTCTCA ACGAGTACTT CTACAGTCAC 
TTGAGCAACC CATATCCATC CGAAGAGGCC AAAGAGGAGT TGGCACGAAA GTGTGGCATC 
ACGGTCTCGC AAGTATCGAA TTGGTTTGGC AACAAGCGTA TTCGCTATAA GAAGAACATT 
GGTAAGGCAC AGGAGGAGGC CAATTTGTAT GCGGCCAAGA AGGCGGCTGG TGCTTCACCG 
TATTCCATGG CCGGTCCACC CAGCGGGACA ACCACACCCA TGATGTCACC CGCTCCGCCA 
CAGGATTCCA TGGGCTATCC AATGGGCTCC GGCGGATACG ACCAGCAGCA ACCGTACGAC 
AACAGCATGG GCGGCTACGA CCCAAATCTC CATCAGGATC TAAGCCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259592.1
CDS	263..1393
Translation	MEDPNRMLAHTGGMMAPQGYGLSGQDDGQNAGSENEVRKQKDIGEILQQIMSISEQSLDEAQARKHTLNCHRMKPALFSVLCEIKEKTVLSIRNTQEEEPPDPQLMRLDNMLIAEGVAGPEKGGGGAAAASAAAASQGGSLSIDGADNAIEHSDYRAKLAQIRQIYHQELEKYEQACNEFTTHVMNLLREQSRTRPITPKEIERMVQIIHKKFSSIQMQLKQSTCEAVMILRSRFLDARRKRRNFSKQASEILNEYFYSHLSNPYPSEEAKEELARKCGITVSQVSNWFGNKRIRYKKNIGKAQEEANLYAAKKAAGASPYSMAGPPSGTTTPMMSPAPPQDSMGYPMGSGGYDQQQPYDNSMGGYDPNLHQDLSP

Target ORF information:

RefSeq Version	NM_001272663.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster extradenticle (exd), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272663.2

ORF Insert Sequence:

ATGGAGGACC CCAATCGCAT GTTGGCCCAC ACCGGCGGCA TGATGGCTCC GCAAGGATAC 
GGCTTGTCTG GCCAGGATGA TGGCCAGAAT GCCGGCAGCG AGAACGAGGT GCGCAAGCAG 
AAGGACATCG GCGAGATATT GCAACAGATA ATGAGTATCT CGGAGCAATC ACTTGACGAG 
GCCCAGGCCA GAAAACATAC ACTAAACTGT CACCGAATGA AGCCGGCACT TTTCTCTGTA 
CTTTGCGAAA TCAAGGAGAA GACCGTCCTC TCGATTCGCA ACACCCAGGA GGAGGAGCCC 
CCAGATCCGC AACTGATGCG CTTGGACAAC ATGCTGATTG CCGAGGGCGT GGCCGGACCC 
GAGAAGGGCG GTGGCGGAGC AGCGGCTGCC TCTGCGGCTG CGGCCAGCCA GGGCGGTTCC 
CTGTCCATCG ATGGTGCTGA CAATGCCATT GAGCATTCCG ACTACCGTGC CAAGCTGGCA 
CAGATCCGTC AAATATATCA CCAGGAATTG GAGAAGTACG AGCAGGCATG CAACGAGTTC 
ACCACGCATG TCATGAATCT TCTACGCGAA CAGAGTCGCA CGAGACCCAT TACACCAAAG 
GAAATCGAGC GAATGGTGCA GATCATCCAC AAAAAGTTTA GTTCCATACA AATGCAGCTG 
AAGCAGTCGA CCTGCGAGGC TGTAATGATA CTTCGTTCGC GTTTCCTGGA CGCACGCCGC 
AAACGCCGCA ATTTCAGCAA GCAGGCATCC GAGATTCTCA ACGAGTACTT CTACAGTCAC 
TTGAGCAACC CATATCCATC CGAAGAGGCC AAAGAGGAGT TGGCACGAAA GTGTGGCATC 
ACGGTCTCGC AAGTATCGAA TTGGTTTGGC AACAAGCGTA TTCGCTATAA GAAGAACATT 
GGTAAGGCAC AGGAGGAGGC CAATTTGTAT GCGGCCAAGA AGGCGGCTGG TGCTTCACCG 
TATTCCATGG CCGGTCCACC CAGCGGGACA ACCACACCCA TGATGTCACC CGCTCCGCCA 
CAGGATTCCA TGGGCTATCC AATGGGCTCC GGCGGATACG ACCAGCAGCA ACCGTACGAC 
AACAGCATGG GCGGCTACGA CCCAAATCTC CATCAGGATC TAAGCCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

exd ( NM_001272664.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259593.1 Drosophila melanogaster extradenticle (exd), transcript variant E, mRNA.

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CloneID	OFa01747
Clone ID Related Accession (Same CDS sequence)	NM_078636.5 , NM_167478.3 , NM_001272663.2 , NM_001272664.1
Accession Version	NM_001272664.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	extradenticle, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGACC CCAATCGCAT GTTGGCCCAC ACCGGCGGCA TGATGGCTCC GCAAGGATAC 
GGCTTGTCTG GCCAGGATGA TGGCCAGAAT GCCGGCAGCG AGAACGAGGT GCGCAAGCAG 
AAGGACATCG GCGAGATATT GCAACAGATA ATGAGTATCT CGGAGCAATC ACTTGACGAG 
GCCCAGGCCA GAAAACATAC ACTAAACTGT CACCGAATGA AGCCGGCACT TTTCTCTGTA 
CTTTGCGAAA TCAAGGAGAA GACCGTCCTC TCGATTCGCA ACACCCAGGA GGAGGAGCCC 
CCAGATCCGC AACTGATGCG CTTGGACAAC ATGCTGATTG CCGAGGGCGT GGCCGGACCC 
GAGAAGGGCG GTGGCGGAGC AGCGGCTGCC TCTGCGGCTG CGGCCAGCCA GGGCGGTTCC 
CTGTCCATCG ATGGTGCTGA CAATGCCATT GAGCATTCCG ACTACCGTGC CAAGCTGGCA 
CAGATCCGTC AAATATATCA CCAGGAATTG GAGAAGTACG AGCAGGCATG CAACGAGTTC 
ACCACGCATG TCATGAATCT TCTACGCGAA CAGAGTCGCA CGAGACCCAT TACACCAAAG 
GAAATCGAGC GAATGGTGCA GATCATCCAC AAAAAGTTTA GTTCCATACA AATGCAGCTG 
AAGCAGTCGA CCTGCGAGGC TGTAATGATA CTTCGTTCGC GTTTCCTGGA CGCACGCCGC 
AAACGCCGCA ATTTCAGCAA GCAGGCATCC GAGATTCTCA ACGAGTACTT CTACAGTCAC 
TTGAGCAACC CATATCCATC CGAAGAGGCC AAAGAGGAGT TGGCACGAAA GTGTGGCATC 
ACGGTCTCGC AAGTATCGAA TTGGTTTGGC AACAAGCGTA TTCGCTATAA GAAGAACATT 
GGTAAGGCAC AGGAGGAGGC CAATTTGTAT GCGGCCAAGA AGGCGGCTGG TGCTTCACCG 
TATTCCATGG CCGGTCCACC CAGCGGGACA ACCACACCCA TGATGTCACC CGCTCCGCCA 
CAGGATTCCA TGGGCTATCC AATGGGCTCC GGCGGATACG ACCAGCAGCA ACCGTACGAC 
AACAGCATGG GCGGCTACGA CCCAAATCTC CATCAGGATC TAAGCCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259593.1
CDS	216..1346
Translation	MEDPNRMLAHTGGMMAPQGYGLSGQDDGQNAGSENEVRKQKDIGEILQQIMSISEQSLDEAQARKHTLNCHRMKPALFSVLCEIKEKTVLSIRNTQEEEPPDPQLMRLDNMLIAEGVAGPEKGGGGAAAASAAAASQGGSLSIDGADNAIEHSDYRAKLAQIRQIYHQELEKYEQACNEFTTHVMNLLREQSRTRPITPKEIERMVQIIHKKFSSIQMQLKQSTCEAVMILRSRFLDARRKRRNFSKQASEILNEYFYSHLSNPYPSEEAKEELARKCGITVSQVSNWFGNKRIRYKKNIGKAQEEANLYAAKKAAGASPYSMAGPPSGTTTPMMSPAPPQDSMGYPMGSGGYDQQQPYDNSMGGYDPNLHQDLSP

Target ORF information:

RefSeq Version	NM_001272664.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster extradenticle (exd), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272664.1

ORF Insert Sequence:

ATGGAGGACC CCAATCGCAT GTTGGCCCAC ACCGGCGGCA TGATGGCTCC GCAAGGATAC 
GGCTTGTCTG GCCAGGATGA TGGCCAGAAT GCCGGCAGCG AGAACGAGGT GCGCAAGCAG 
AAGGACATCG GCGAGATATT GCAACAGATA ATGAGTATCT CGGAGCAATC ACTTGACGAG 
GCCCAGGCCA GAAAACATAC ACTAAACTGT CACCGAATGA AGCCGGCACT TTTCTCTGTA 
CTTTGCGAAA TCAAGGAGAA GACCGTCCTC TCGATTCGCA ACACCCAGGA GGAGGAGCCC 
CCAGATCCGC AACTGATGCG CTTGGACAAC ATGCTGATTG CCGAGGGCGT GGCCGGACCC 
GAGAAGGGCG GTGGCGGAGC AGCGGCTGCC TCTGCGGCTG CGGCCAGCCA GGGCGGTTCC 
CTGTCCATCG ATGGTGCTGA CAATGCCATT GAGCATTCCG ACTACCGTGC CAAGCTGGCA 
CAGATCCGTC AAATATATCA CCAGGAATTG GAGAAGTACG AGCAGGCATG CAACGAGTTC 
ACCACGCATG TCATGAATCT TCTACGCGAA CAGAGTCGCA CGAGACCCAT TACACCAAAG 
GAAATCGAGC GAATGGTGCA GATCATCCAC AAAAAGTTTA GTTCCATACA AATGCAGCTG 
AAGCAGTCGA CCTGCGAGGC TGTAATGATA CTTCGTTCGC GTTTCCTGGA CGCACGCCGC 
AAACGCCGCA ATTTCAGCAA GCAGGCATCC GAGATTCTCA ACGAGTACTT CTACAGTCAC 
TTGAGCAACC CATATCCATC CGAAGAGGCC AAAGAGGAGT TGGCACGAAA GTGTGGCATC 
ACGGTCTCGC AAGTATCGAA TTGGTTTGGC AACAAGCGTA TTCGCTATAA GAAGAACATT 
GGTAAGGCAC AGGAGGAGGC CAATTTGTAT GCGGCCAAGA AGGCGGCTGG TGCTTCACCG 
TATTCCATGG CCGGTCCACC CAGCGGGACA ACCACACCCA TGATGTCACC CGCTCCGCCA 
CAGGATTCCA TGGGCTATCC AATGGGCTCC GGCGGATACG ACCAGCAGCA ACCGTACGAC 
AACAGCATGG GCGGCTACGA CCCAAATCTC CATCAGGATC TAAGCCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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exd cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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