PGRP-LE cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	PGRP-LE
Entrez Gene ID	32534
Full Name	Peptidoglycan recognition protein LE
Synonyms	CG8995,Dmel\CG8995,PGRP,anon-WO0153538.66,pgrp le,pgrp-le
General protein information	Preferred Names Peptidoglycan recognition protein LE Names CG8995 gene product from transcript CG8995-RB CG8995-PA CG8995-PB PGRP-LE-PA PGRP-LE-PB peptidoglycan recognition protein peptidoglycan recognition protein LE peptidoglycan recognition protein-LE
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 13F1-13F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001258766.3	NP_001245695.1	peptidoglycan recognition protein LE, isoform B
NM_132850.4	NP_573078.1	peptidoglycan recognition protein LE, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Protofibrils of peptidoglycan receptors PGRP-LC and PGRP-LE seed immune deficiency protein (Imd) amyloid polymerization via Imd RIP homotypic interaction motif (RHIM), and the formation of functional Imd amyloid fibril is required for NF-kappaB signaling.
Title: Peptidoglycan-Sensing Receptors Trigger the Formation of Functional Amyloids of the Adaptor Protein Imd to Initiate Drosophila NF-?B Signaling.

our study delineates how a combination of extracellular sensing by PGRP-LC isoforms and intracellular sensing through PGRP-LE provides sophisticated mechanisms to detect and differentiate between infections in Drosophila.
Title: Tissue- and ligand-specific sensing of gram-negative infection in drosophila by PGRP-LC isoforms and PGRP-LE.

PGRP-LE functions as a gut bacterial sensor that induces balanced responses to infectious bacteria and tolerance to microbiota.
Title: Peptidoglycan sensing by the receptor PGRP-LE in the Drosophila gut induces immune responses to infectious bacteria and tolerance to microbiota.

Listericin gene encodes a novel antibacterial peptide-like protein whose induction is cooperatively regulated by PGRP-LE and the JAK-STAT pathway.
Title: Cooperative regulation of the induction of the novel antibacterial Listericin by peptidoglycan recognition protein LE and the JAK-STAT pathway.

PGRP-LE and PGRP-LC play a crucial role in detecting extracellular and intracellular bacteria with DAP-type peptidoglycans to induce various innate immune responses
Title: Extracellular and intracellular pathogen recognition by Drosophila PGRP-LE and PGRP-LC.

The following PGRP-LE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PGRP-LE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa01717	NM_001258766.3 Latest version!	Drosophila melanogaster peptidoglycan recognition protein LE, transcript variant B (PGRP-LE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa01717	NM_132850.4 Latest version!	Drosophila melanogaster peptidoglycan recognition protein LE, transcript variant A (PGRP-LE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PGRP-LE ( NM_001258766.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245695.1 Drosophila melanogaster peptidoglycan recognition protein LE, transcript variant B (PGRP-LE), mRNA.

Price & Availability↑

CloneID	OFa01717
Clone ID Related Accession (Same CDS sequence)	NM_001258766.3 , NM_132850.4
Accession Version	NM_001258766.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1038bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	peptidoglycan recognition protein LE, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001258766.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCGAAT CGGGAATCAA AAAGTTGAGC CAGGAGCGGA CTCGCGAATG GTTGGCTAGT 
CAGGAGGACG AGGAACTGGA GTCCATTGCA GAGTCCTCGG TTGTGGACAG CTTGGACTAC 
GATTATACCG AGGAAGAGGA GGATGCCGAC CAAAATACCA GTGAAGAAAT CAGCACTATG 
ACACTAGGCA CTCAAATCGC TACCAAAAAG CATTCGATCA TCAGCGACAC CATAAGGGAC 
CTTATGAACT CGATCAACAG CATTCAGACT TTGGGCAACG TTAATATAAG CAACTCCACG 
AACGTCCATA TCGGCAATGT TACCAATATT AATGGAAATA TACAAATCAT AGCCGATGGC 
CTTACTCAAA ACCGAAGAGA TCGGCGGCAT GTTTCACCAC CGAGAGATAA CGCTCCCAAA 
ACTCCGACAC ATTTCGAAGA CGACTATCAG GACGAGTCGG AGGAGAGGGT GCGCTCGGAT 
GTGTTCATCA GGCGCCAGAA ATTCAAAATA CCCAAGGAGC TGTCCGCCAT CATTCCGCGC 
TCTTCGTGGC TAGCACAGAA GCCCATGGAC GAGCCACTGC CCTTGCAACT GCCTGTTAAA 
TATGTGGTTA TCTTACACAC AGCTACAGAG AGTTCAGAAA AGCGGGCGAT CAACGTAAGA 
CTTATCCGTG ACATGCAGTG CTTCCACATT GAGTCGCGGG GATGGAATGA CATTGCGTAC 
AACTTCCTGG TCGGCTGCGA TGGCAACATC TACGAGGGAC GCGGATGGAA AACGGTGGGC 
GCCCACACTC TGGGTTACAA CAGGATCTCG CTGGGGATCA GTTTCATTGG ATGCTTCATG 
AAAGAACTCC CGACGGCAGA CGCCTTGAAT ATGTGCCGCA ATCTGTTGGC CCGCGGCGTT 
GAGGATGGTC ACATATCCAC CGACTATAGA TTAATTTGTC ACTGCCAGTG CAATTCGACG 
GAGAGTCCTG GTCGGCGATT GTACGAGGAG ATACAGACAT GGCCCCACTT TTACAATATC 
GAGGAGGAGG AACAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245695.1
CDS	938..1975
Translation	MSESGIKKLSQERTREWLASQEDEELESIAESSVVDSLDYDYTEEEEDADQNTSEEISTMTLGTQIATKKHSIISDTIRDLMNSINSIQTLGNVNISNSTNVHIGNVTNINGNIQIIADGLTQNRRDRRHVSPPRDNAPKTPTHFEDDYQDESEERVRSDVFIRRQKFKIPKELSAIIPRSSWLAQKPMDEPLPLQLPVKYVVILHTATESSEKRAINVRLIRDMQCFHIESRGWNDIAYNFLVGCDGNIYEGRGWKTVGAHTLGYNRISLGISFIGCFMKELPTADALNMCRNLLARGVEDGHISTDYRLICHCQCNSTESPGRRLYEEIQTWPHFYNIEEEEQ

Target ORF information:

RefSeq Version	NM_001258766.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster peptidoglycan recognition protein LE, transcript variant B (PGRP-LE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258766.3

ORF Insert Sequence:

ATGTCCGAAT CGGGAATCAA AAAGTTGAGC CAGGAGCGGA CTCGCGAATG GTTGGCTAGT 
CAGGAGGACG AGGAACTGGA GTCCATTGCA GAGTCCTCGG TTGTGGACAG CTTGGACTAC 
GATTATACCG AGGAAGAGGA GGATGCCGAC CAAAATACCA GTGAAGAAAT CAGCACTATG 
ACACTAGGCA CTCAAATCGC TACCAAAAAG CATTCGATCA TCAGCGACAC CATAAGGGAC 
CTTATGAACT CGATCAACAG CATTCAGACT TTGGGCAACG TTAATATAAG CAACTCCACG 
AACGTCCATA TCGGCAATGT TACCAATATT AATGGAAATA TACAAATCAT AGCCGATGGC 
CTTACTCAAA ACCGAAGAGA TCGGCGGCAT GTTTCACCAC CGAGAGATAA CGCTCCCAAA 
ACTCCGACAC ATTTCGAAGA CGACTATCAG GACGAGTCGG AGGAGAGGGT GCGCTCGGAT 
GTGTTCATCA GGCGCCAGAA ATTCAAAATA CCCAAGGAGC TGTCCGCCAT CATTCCGCGC 
TCTTCGTGGC TAGCACAGAA GCCCATGGAC GAGCCACTGC CCTTGCAACT GCCTGTTAAA 
TATGTGGTTA TCTTACACAC AGCTACAGAG AGTTCAGAAA AGCGGGCGAT CAACGTAAGA 
CTTATCCGTG ACATGCAGTG CTTCCACATT GAGTCGCGGG GATGGAATGA CATTGCGTAC 
AACTTCCTGG TCGGCTGCGA TGGCAACATC TACGAGGGAC GCGGATGGAA AACGGTGGGC 
GCCCACACTC TGGGTTACAA CAGGATCTCG CTGGGGATCA GTTTCATTGG ATGCTTCATG 
AAAGAACTCC CGACGGCAGA CGCCTTGAAT ATGTGCCGCA ATCTGTTGGC CCGCGGCGTT 
GAGGATGGTC ACATATCCAC CGACTATAGA TTAATTTGTC ACTGCCAGTG CAATTCGACG 
GAGAGTCCTG GTCGGCGATT GTACGAGGAG ATACAGACAT GGCCCCACTT TTACAATATC 
GAGGAGGAGG AACAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PGRP-LE ( NM_132850.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_573078.1 Drosophila melanogaster peptidoglycan recognition protein LE, transcript variant A (PGRP-LE), mRNA.

Price & Availability↑

CloneID	OFa01717
Clone ID Related Accession (Same CDS sequence)	NM_001258766.3 , NM_132850.4
Accession Version	NM_132850.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1038bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	peptidoglycan recognition protein LE, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_132850.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCGAAT CGGGAATCAA AAAGTTGAGC CAGGAGCGGA CTCGCGAATG GTTGGCTAGT 
CAGGAGGACG AGGAACTGGA GTCCATTGCA GAGTCCTCGG TTGTGGACAG CTTGGACTAC 
GATTATACCG AGGAAGAGGA GGATGCCGAC CAAAATACCA GTGAAGAAAT CAGCACTATG 
ACACTAGGCA CTCAAATCGC TACCAAAAAG CATTCGATCA TCAGCGACAC CATAAGGGAC 
CTTATGAACT CGATCAACAG CATTCAGACT TTGGGCAACG TTAATATAAG CAACTCCACG 
AACGTCCATA TCGGCAATGT TACCAATATT AATGGAAATA TACAAATCAT AGCCGATGGC 
CTTACTCAAA ACCGAAGAGA TCGGCGGCAT GTTTCACCAC CGAGAGATAA CGCTCCCAAA 
ACTCCGACAC ATTTCGAAGA CGACTATCAG GACGAGTCGG AGGAGAGGGT GCGCTCGGAT 
GTGTTCATCA GGCGCCAGAA ATTCAAAATA CCCAAGGAGC TGTCCGCCAT CATTCCGCGC 
TCTTCGTGGC TAGCACAGAA GCCCATGGAC GAGCCACTGC CCTTGCAACT GCCTGTTAAA 
TATGTGGTTA TCTTACACAC AGCTACAGAG AGTTCAGAAA AGCGGGCGAT CAACGTAAGA 
CTTATCCGTG ACATGCAGTG CTTCCACATT GAGTCGCGGG GATGGAATGA CATTGCGTAC 
AACTTCCTGG TCGGCTGCGA TGGCAACATC TACGAGGGAC GCGGATGGAA AACGGTGGGC 
GCCCACACTC TGGGTTACAA CAGGATCTCG CTGGGGATCA GTTTCATTGG ATGCTTCATG 
AAAGAACTCC CGACGGCAGA CGCCTTGAAT ATGTGCCGCA ATCTGTTGGC CCGCGGCGTT 
GAGGATGGTC ACATATCCAC CGACTATAGA TTAATTTGTC ACTGCCAGTG CAATTCGACG 
GAGAGTCCTG GTCGGCGATT GTACGAGGAG ATACAGACAT GGCCCCACTT TTACAATATC 
GAGGAGGAGG AACAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_573078.1
CDS	263..1300
Translation	MSESGIKKLSQERTREWLASQEDEELESIAESSVVDSLDYDYTEEEEDADQNTSEEISTMTLGTQIATKKHSIISDTIRDLMNSINSIQTLGNVNISNSTNVHIGNVTNINGNIQIIADGLTQNRRDRRHVSPPRDNAPKTPTHFEDDYQDESEERVRSDVFIRRQKFKIPKELSAIIPRSSWLAQKPMDEPLPLQLPVKYVVILHTATESSEKRAINVRLIRDMQCFHIESRGWNDIAYNFLVGCDGNIYEGRGWKTVGAHTLGYNRISLGISFIGCFMKELPTADALNMCRNLLARGVEDGHISTDYRLICHCQCNSTESPGRRLYEEIQTWPHFYNIEEEEQ

Target ORF information:

RefSeq Version	NM_132850.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster peptidoglycan recognition protein LE, transcript variant A (PGRP-LE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132850.4

ORF Insert Sequence:

ATGTCCGAAT CGGGAATCAA AAAGTTGAGC CAGGAGCGGA CTCGCGAATG GTTGGCTAGT 
CAGGAGGACG AGGAACTGGA GTCCATTGCA GAGTCCTCGG TTGTGGACAG CTTGGACTAC 
GATTATACCG AGGAAGAGGA GGATGCCGAC CAAAATACCA GTGAAGAAAT CAGCACTATG 
ACACTAGGCA CTCAAATCGC TACCAAAAAG CATTCGATCA TCAGCGACAC CATAAGGGAC 
CTTATGAACT CGATCAACAG CATTCAGACT TTGGGCAACG TTAATATAAG CAACTCCACG 
AACGTCCATA TCGGCAATGT TACCAATATT AATGGAAATA TACAAATCAT AGCCGATGGC 
CTTACTCAAA ACCGAAGAGA TCGGCGGCAT GTTTCACCAC CGAGAGATAA CGCTCCCAAA 
ACTCCGACAC ATTTCGAAGA CGACTATCAG GACGAGTCGG AGGAGAGGGT GCGCTCGGAT 
GTGTTCATCA GGCGCCAGAA ATTCAAAATA CCCAAGGAGC TGTCCGCCAT CATTCCGCGC 
TCTTCGTGGC TAGCACAGAA GCCCATGGAC GAGCCACTGC CCTTGCAACT GCCTGTTAAA 
TATGTGGTTA TCTTACACAC AGCTACAGAG AGTTCAGAAA AGCGGGCGAT CAACGTAAGA 
CTTATCCGTG ACATGCAGTG CTTCCACATT GAGTCGCGGG GATGGAATGA CATTGCGTAC 
AACTTCCTGG TCGGCTGCGA TGGCAACATC TACGAGGGAC GCGGATGGAA AACGGTGGGC 
GCCCACACTC TGGGTTACAA CAGGATCTCG CTGGGGATCA GTTTCATTGG ATGCTTCATG 
AAAGAACTCC CGACGGCAGA CGCCTTGAAT ATGTGCCGCA ATCTGTTGGC CCGCGGCGTT 
GAGGATGGTC ACATATCCAC CGACTATAGA TTAATTTGTC ACTGCCAGTG CAATTCGACG 
GAGAGTCCTG GTCGGCGATT GTACGAGGAG ATACAGACAT GGCCCCACTT TTACAATATC 
GAGGAGGAGG AACAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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PGRP-LE cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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