Graf cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Graf
Entrez Gene ID	32522
Full Name	GTPase regulator associated with FAK
Synonyms	CG8948,D-Graf,Dmel\CG8948,Q9VXQ2_DROME
General protein information	Preferred Names GTPase regulator associated with FAK Names CG8948 gene product from transcript CG8948-RJ CG8948-PA CG8948-PD CG8948-PE CG8948-PG CG8948-PH CG8948-PI CG8948-PJ GRAF ortholog GTPase regulator associated with focal adhesion kinase ortholog Graf-PA Graf-PD Graf-PE Graf-PG Graf-PH Graf-PI Graf-PJ
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 13E14-13E14

mRNA and Protein(s)

mRNA	Protein	Name
NM_206719.2	NP_996442.1	GTPase regulator associated with FAK, isoform E
NM_206718.2	NP_996441.2	GTPase regulator associated with FAK, isoform G
NM_206722.2	NP_996445.1	GTPase regulator associated with FAK, isoform J
NM_206721.2	NP_996444.2	GTPase regulator associated with FAK, isoform I
NM_206720.2	NP_996443.1	GTPase regulator associated with FAK, isoform D
NM_132842.3	NP_573070.2	GTPase regulator associated with FAK, isoform A
NM_001298367.1	NP_001285296.1	GTPase regulator associated with FAK, isoform H

Pathways from BioSystems

Homologies

	ARHGAP26	XP_001096464.2
Drosophila melanogaster (fruit fly)	Graf	NP_996441.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP013143	XP_003436889.1
Caenorhabditis elegans (roundworm)	T04C9.1	NP_741163.2
Homo sapiens (human)	ARHGAP26	NP_055886.1
Mus musculus (house mouse)	Arhgap26	NP_780373.3
Xenopus tropicalis (tropical clawed frog)	arhgap26	NP_001120245.2
Pan troglodytes (chimpanzee)	ARHGAP26	XP_001154393.1
Canis lupus familiaris (dog)	ARHGAP26	XP_535224.4
Bos taurus (cattle)	ARHGAP26	NP_001192451.1
Rattus norvegicus (Norway rat)	Arhgap26	NP_001100859.1
Gallus gallus (chicken)	ARHGAP26	NP_990525.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Graf functions to downregulate EGFR signaling.
Title: Graf regulates hematopoiesis through GEEC endocytosis of EGFR.

The following Graf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Graf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa03599	NM_206722.2 Latest version!	Drosophila melanogaster GTPase regulator associated with FAK (Graf), transcript variant J, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa03599	NM_206719.2 Latest version!	Drosophila melanogaster GTPase regulator associated with FAK (Graf), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa03599	NM_206720.2 Latest version!	Drosophila melanogaster GTPase regulator associated with FAK (Graf), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa03599	NM_132842.3 Latest version!	Drosophila melanogaster GTPase regulator associated with FAK (Graf), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa21492	NM_001298367.1 Latest version!	Drosophila melanogaster GTPase regulator associated with FAK (Graf), transcript variant H, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa21492	NM_206721.2 Latest version!	Drosophila melanogaster GTPase regulator associated with FAK (Graf), transcript variant I, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa21492	NM_206718.2 Latest version!	Drosophila melanogaster GTPase regulator associated with FAK (Graf), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Graf ( NM_206719.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996442.1 Drosophila melanogaster GTPase regulator associated with FAK (Graf), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa03599
Clone ID Related Accession (Same CDS sequence)	NM_206719.2 , NM_206722.2 , NM_206720.2 , NM_132842.3
Accession Version	NM_206719.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3051bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	GTPase regulator associated with FAK, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_206719.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCGGCG GCAAAAATGT ACGCCGCGGC TTGGAGCCTC TGGAGTTCGA AGAATGCATC 
GTCGACAGTC CGGACTTTCG CGAAAATTTA AATCGACATG AAAAGGAACT GGACCACACT 
AGTCATCAAA TAAAACGAAT TATCAAAGAG GTCAAAGATC TTATGAGTGC GGCAAAGATC 
CTGTCCACTC GAATGAAACA ATTGGCCATT TTACTTAACG ATTTTAACTT TGAGTGCATT 
GGCACTGCTC AGACCGACGA TGAGAATGTC ATTTGTGAAA GTCTCAAGCG ATTTGGTGCT 
ATAATTGGCA ATATTGAAGA TGAGCGCGAA AAAATGCTAA CGCTCGCCGA TAAACATATT 
ATTGAGTCAC TGGAGGATTT TCGAAAAAAG CAAATCGGTG GCGTCAAAGA AAACAAAAAG 
AAATTTGATA AAAAGACGGA GAAATTTTGT CAATCGCAGG AGCGTTTCCT CAATATGTCA 
ACTAAAAAGC CCGAAAACAC AATACAGGAG GCTGACGCCA GTTTGGGGAT GCATGAACGC 
GAGTATATCC AGGAGTCGCT GAGCTATGTG CTGCGCATCC AAGAAGTGCA GGAACGAATC 
AAATTCGAAT TTGTCGAAAT ATTGCTAGCA TTTATCTCTG GCTGGCTGGT TTTCTACCAC 
ACGGCGCACG AACAGGCGGA GGATCATCGT GATTATCTGC AGGATTTGAG GCACAAAGTT 
CAAAAAACTC GGGAAAATTT CGAGGAGGCA CGCGAAAAAG TGACTGAATT GAAAACAAAA 
TATATGGAAA AACGCACAAA GCCCGAGGAA ATTTTTACAA AACGTGGCTA CCTATTTCTA 
ATGGAGAAAA AGCCATTCAA AGCCACCTGG ACAAAATACT ATTGTACATT CAAGAAGCAA 
AAGCGCGAGT TCACTATGCT CCAGTTCAAC CAGATGAATC ACAACTTCAC GAGACCCGAG 
GCGCGTGACG ACGAGAAACT GACGCTTTTC TCCTGCCAGC GACGGGCATC TGAGTTCGAG 
AAGAGGTTCT GCTTCGATTT GACCTTCAAG GAGAAGCCAG GCGTCGTCTA CACATTTCAG 
GCTTTGAGCG AAAAGGATCA TCGCTATTGG ATTAGTGCCA TGGATGGCAC GGAGCCGACA 
TATCTGGCTC CCGGCAAAAT CAAAGTCAGC GAGGCATACC ATCTGGATGA AGCCGGATTC 
ATGTTCATCC GCAGATGCAT TCAAGTATTA GAGATTCGAG GCCTGGAGGA TGAGGGCATA 
TACAGAAAGA GCGGTGTTGG AACCAAAATC AGCAAGCTGC TAGCACTGGG CCTCAATCAA 
AAGGAGAGCG ACGATGTCTT CGTCGACGAC AAGTACCGCG ATTTGATGGA AAGCAACACC 
ATAGCTAGTG CCCTAAAAAT GTATCTTCGA AATCTGAATG AGCCATTGAT GACGTATCAA 
TATCATAGTG ATTTCATTGA AGCCGCAAAA CAAGAAACCC TCAATCAGCG CGTGAATGAG 
GTGCACAAGC TGGTCTACAA GTTGCCGCAG CCCAATTTTC AGATGCTCGA CATGGTTATT 
TGCCATCTGA CTGACGTTTC GCGCAAGTAC GAGAAGAACA AAATGTCCGT ATTTAATCTG 
GGAGTGGTGT TTGGGCCAAC ATTGTTGCGT CCTCGAGAGG AATCAGTTGC CGCCATATTG 
GATATTAAGT TTAATAATAT CGTCATCAAC ATTCTAATTG ATAACTACGA GAGGATATTC 
AAGAACAAGC CCAGCGCCGA TATCAAGTTG CCGGACGCCA CGAAGGCACC CAGCATCATG 
TACCCCAGTC GCAGCAGTCC ACCGACCAGA ATGCCGCGAG CCTCTCAAAT CGGCAAGGCC 
GCTTCAACGG GTGCAATGGG TAGCAGCAGT AATACAGCGG GCAATCCGAT GTTCTTGAAT 
CAGCAGAAAA TCTATCGCGT TGTGAGCAAA ACCAACTGCA CCGAGCCCAC AATGTCATCC 
AGCCTGCAAA ATATTCCGAA TGGCGATAAC TATGCCCTTG GCTCGAACGC CATGAATAGC 
TCGGGCGGTG CTCAATGCAT ATCCCTGTCG CCGCCGATGC ACATGCTGAA CGGCATACTC 
TCGCCGACCA TTGGTAGCAT CAATAATCTG CACACAATAT CGAAAAATGA GACCAGCACA 
CGACGATACG TGCCCGACAC TGAGATTGCG CCCGATTATG GTATTATAGG TGCTAACAAC 
GGCGGTGTGA CGCTGCAATC GCATCACGCT GTGCCGGTTA GTAGCACGAG TAACTTTCGA 
CATCCCGAGT ACCTAATGAC CACGGCCAAT CCAGTTACTC AATCGGGCTC AAGTAGCCAT 
ATATATACGA ACACATCTAG TGCTGGTGCT AACTCAAGCA ATCGGATTAG TCTAAGCAAT 
GTATCCCCGC CAAATACTGC AATGCGCAAG GAGCGATTCC TGGGCAGCGC CAGTGGTCCG 
CAGCAGCATC CGCCCGTTCA ACGGGGTCTG CACTCGTACG GCCAGACAAA ACACTATTCA 
CCCCTAATGC CAACGTCAAC ATCCAGCTCC AACGACAGCG TATGTGATTC ACTATCTTCC 
AACAACGGCC TGGGCAGCTC CATTGTGGCC AATAGTGGCA ACAGTGGGAA TGTTGCCCAG 
CACTTGAGCG CACGGAACAC TAATGACTAT GCGCTGATAT CGTCACAGAA TTCATCGCTG 
TCGCCGCATC TTGGACCCAC CTGCGACGAG GTCGTCACGG CCACAACATT GCCCTCGGTT 
AGTTTAAGCT GTGGTGGCAC CGCCAGCGAA AGCACCGAAT ATCCGCCTAG TAAAATGCAT 
CGTAATCGTG ACGTCAACCA AATAAAACGT GATTTGTCAA CGGGCACCGC TCGTGTGCGC 
ACACTCTATG CTTGCATGGG AGAAAGCGAG GGCGAGCTTT CTTTCGAGCC CAACCAAATA 
ATAACCAATG TTCGTTACTC CCACGAACCC GGCTGGCTGC AGGGCACACT AAATGGGAAA 
ACAGGACTCA TTCCGGAGAA CTATGTGGAA CATTTGAAGC CGCACCATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996442.1
CDS	695..3745
Translation	MGGGKNVRRGLEPLEFEECIVDSPDFRENLNRHEKELDHTSHQIKRIIKEVKDLMSAAKILSTRMKQLAILLNDFNFECIGTAQTDDENVICESLKRFGAIIGNIEDEREKMLTLADKHIIESLEDFRKKQIGGVKENKKKFDKKTEKFCQSQERFLNMSTKKPENTIQEADASLGMHEREYIQESLSYVLRIQEVQERIKFEFVEILLAFISGWLVFYHTAHEQAEDHRDYLQDLRHKVQKTRENFEEAREKVTELKTKYMEKRTKPEEIFTKRGYLFLMEKKPFKATWTKYYCTFKKQKREFTMLQFNQMNHNFTRPEARDDEKLTLFSCQRRASEFEKRFCFDLTFKEKPGVVYTFQALSEKDHRYWISAMDGTEPTYLAPGKIKVSEAYHLDEAGFMFIRRCIQVLEIRGLEDEGIYRKSGVGTKISKLLALGLNQKESDDVFVDDKYRDLMESNTIASALKMYLRNLNEPLMTYQYHSDFIEAAKQETLNQRVNEVHKLVYKLPQPNFQMLDMVICHLTDVSRKYEKNKMSVFNLGVVFGPTLLRPREESVAAILDIKFNNIVINILIDNYERIFKNKPSADIKLPDATKAPSIMYPSRSSPPTRMPRASQIGKAASTGAMGSSSNTAGNPMFLNQQKIYRVVSKTNCTEPTMSSSLQNIPNGDNYALGSNAMNSSGGAQCISLSPPMHMLNGILSPTIGSINNLHTISKNETSTRRYVPDTEIAPDYGIIGANNGGVTLQSHHAVPVSSTSNFRHPEYLMTTANPVTQSGSSSHIYTNTSSAGANSSNRISLSNVSPPNTAMRKERFLGSASGPQQHPPVQRGLHSYGQTKHYSPLMPTSTSSSNDSVCDSLSSNNGLGSSIVANSGNSGNVAQHLSARNTNDYALISSQNSSLSPHLGPTCDEVVTATTLPSVSLSCGGTASESTEYPPSKMHRNRDVNQIKRDLSTGTARVRTLYACMGESEGELSFEPNQIITNVRYSHEPGWLQGTLNGKTGLIPENYVEHLKPHH

Target ORF information:

RefSeq Version	NM_206719.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster GTPase regulator associated with FAK (Graf), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206719.2

ORF Insert Sequence:

ATGGGCGGCG GCAAAAATGT ACGCCGCGGC TTGGAGCCTC TGGAGTTCGA AGAATGCATC 
GTCGACAGTC CGGACTTTCG CGAAAATTTA AATCGACATG AAAAGGAACT GGACCACACT 
AGTCATCAAA TAAAACGAAT TATCAAAGAG GTCAAAGATC TTATGAGTGC GGCAAAGATC 
CTGTCCACTC GAATGAAACA ATTGGCCATT TTACTTAACG ATTTTAACTT TGAGTGCATT 
GGCACTGCTC AGACCGACGA TGAGAATGTC ATTTGTGAAA GTCTCAAGCG ATTTGGTGCT 
ATAATTGGCA ATATTGAAGA TGAGCGCGAA AAAATGCTAA CGCTCGCCGA TAAACATATT 
ATTGAGTCAC TGGAGGATTT TCGAAAAAAG CAAATCGGTG GCGTCAAAGA AAACAAAAAG 
AAATTTGATA AAAAGACGGA GAAATTTTGT CAATCGCAGG AGCGTTTCCT CAATATGTCA 
ACTAAAAAGC CCGAAAACAC AATACAGGAG GCTGACGCCA GTTTGGGGAT GCATGAACGC 
GAGTATATCC AGGAGTCGCT GAGCTATGTG CTGCGCATCC AAGAAGTGCA GGAACGAATC 
AAATTCGAAT TTGTCGAAAT ATTGCTAGCA TTTATCTCTG GCTGGCTGGT TTTCTACCAC 
ACGGCGCACG AACAGGCGGA GGATCATCGT GATTATCTGC AGGATTTGAG GCACAAAGTT 
CAAAAAACTC GGGAAAATTT CGAGGAGGCA CGCGAAAAAG TGACTGAATT GAAAACAAAA 
TATATGGAAA AACGCACAAA GCCCGAGGAA ATTTTTACAA AACGTGGCTA CCTATTTCTA 
ATGGAGAAAA AGCCATTCAA AGCCACCTGG ACAAAATACT ATTGTACATT CAAGAAGCAA 
AAGCGCGAGT TCACTATGCT CCAGTTCAAC CAGATGAATC ACAACTTCAC GAGACCCGAG 
GCGCGTGACG ACGAGAAACT GACGCTTTTC TCCTGCCAGC GACGGGCATC TGAGTTCGAG 
AAGAGGTTCT GCTTCGATTT GACCTTCAAG GAGAAGCCAG GCGTCGTCTA CACATTTCAG 
GCTTTGAGCG AAAAGGATCA TCGCTATTGG ATTAGTGCCA TGGATGGCAC GGAGCCGACA 
TATCTGGCTC CCGGCAAAAT CAAAGTCAGC GAGGCATACC ATCTGGATGA AGCCGGATTC 
ATGTTCATCC GCAGATGCAT TCAAGTATTA GAGATTCGAG GCCTGGAGGA TGAGGGCATA 
TACAGAAAGA GCGGTGTTGG AACCAAAATC AGCAAGCTGC TAGCACTGGG CCTCAATCAA 
AAGGAGAGCG ACGATGTCTT CGTCGACGAC AAGTACCGCG ATTTGATGGA AAGCAACACC 
ATAGCTAGTG CCCTAAAAAT GTATCTTCGA AATCTGAATG AGCCATTGAT GACGTATCAA 
TATCATAGTG ATTTCATTGA AGCCGCAAAA CAAGAAACCC TCAATCAGCG CGTGAATGAG 
GTGCACAAGC TGGTCTACAA GTTGCCGCAG CCCAATTTTC AGATGCTCGA CATGGTTATT 
TGCCATCTGA CTGACGTTTC GCGCAAGTAC GAGAAGAACA AAATGTCCGT ATTTAATCTG 
GGAGTGGTGT TTGGGCCAAC ATTGTTGCGT CCTCGAGAGG AATCAGTTGC CGCCATATTG 
GATATTAAGT TTAATAATAT CGTCATCAAC ATTCTAATTG ATAACTACGA GAGGATATTC 
AAGAACAAGC CCAGCGCCGA TATCAAGTTG CCGGACGCCA CGAAGGCACC CAGCATCATG 
TACCCCAGTC GCAGCAGTCC ACCGACCAGA ATGCCGCGAG CCTCTCAAAT CGGCAAGGCC 
GCTTCAACGG GTGCAATGGG TAGCAGCAGT AATACAGCGG GCAATCCGAT GTTCTTGAAT 
CAGCAGAAAA TCTATCGCGT TGTGAGCAAA ACCAACTGCA CCGAGCCCAC AATGTCATCC 
AGCCTGCAAA ATATTCCGAA TGGCGATAAC TATGCCCTTG GCTCGAACGC CATGAATAGC 
TCGGGCGGTG CTCAATGCAT ATCCCTGTCG CCGCCGATGC ACATGCTGAA CGGCATACTC 
TCGCCGACCA TTGGTAGCAT CAATAATCTG CACACAATAT CGAAAAATGA GACCAGCACA 
CGACGATACG TGCCCGACAC TGAGATTGCG CCCGATTATG GTATTATAGG TGCTAACAAC 
GGCGGTGTGA CGCTGCAATC GCATCACGCT GTGCCGGTTA GTAGCACGAG TAACTTTCGA 
CATCCCGAGT ACCTAATGAC CACGGCCAAT CCAGTTACTC AATCGGGCTC AAGTAGCCAT 
ATATATACGA ACACATCTAG TGCTGGTGCT AACTCAAGCA ATCGGATTAG TCTAAGCAAT 
GTATCCCCGC CAAATACTGC AATGCGCAAG GAGCGATTCC TGGGCAGCGC CAGTGGTCCG 
CAGCAGCATC CGCCCGTTCA ACGGGGTCTG CACTCGTACG GCCAGACAAA ACACTATTCA 
CCCCTAATGC CAACGTCAAC ATCCAGCTCC AACGACAGCG TATGTGATTC ACTATCTTCC 
AACAACGGCC TGGGCAGCTC CATTGTGGCC AATAGTGGCA ACAGTGGGAA TGTTGCCCAG 
CACTTGAGCG CACGGAACAC TAATGACTAT GCGCTGATAT CGTCACAGAA TTCATCGCTG 
TCGCCGCATC TTGGACCCAC CTGCGACGAG GTCGTCACGG CCACAACATT GCCCTCGGTT 
AGTTTAAGCT GTGGTGGCAC CGCCAGCGAA AGCACCGAAT ATCCGCCTAG TAAAATGCAT 
CGTAATCGTG ACGTCAACCA AATAAAACGT GATTTGTCAA CGGGCACCGC TCGTGTGCGC 
ACACTCTATG CTTGCATGGG AGAAAGCGAG GGCGAGCTTT CTTTCGAGCC CAACCAAATA 
ATAACCAATG TTCGTTACTC CCACGAACCC GGCTGGCTGC AGGGCACACT AAATGGGAAA 
ACAGGACTCA TTCCGGAGAA CTATGTGGAA CATTTGAAGC CGCACCATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Graf ( NM_206722.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996445.1 Drosophila melanogaster GTPase regulator associated with FAK (Graf), transcript variant J, mRNA.

Price & Availability↑

CloneID	OFa03599
Clone ID Related Accession (Same CDS sequence)	NM_206719.2 , NM_206722.2 , NM_206720.2 , NM_132842.3
Accession Version	NM_206722.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3051bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	GTPase regulator associated with FAK, isoform J
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_206722.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCGGCG GCAAAAATGT ACGCCGCGGC TTGGAGCCTC TGGAGTTCGA AGAATGCATC 
GTCGACAGTC CGGACTTTCG CGAAAATTTA AATCGACATG AAAAGGAACT GGACCACACT 
AGTCATCAAA TAAAACGAAT TATCAAAGAG GTCAAAGATC TTATGAGTGC GGCAAAGATC 
CTGTCCACTC GAATGAAACA ATTGGCCATT TTACTTAACG ATTTTAACTT TGAGTGCATT 
GGCACTGCTC AGACCGACGA TGAGAATGTC ATTTGTGAAA GTCTCAAGCG ATTTGGTGCT 
ATAATTGGCA ATATTGAAGA TGAGCGCGAA AAAATGCTAA CGCTCGCCGA TAAACATATT 
ATTGAGTCAC TGGAGGATTT TCGAAAAAAG CAAATCGGTG GCGTCAAAGA AAACAAAAAG 
AAATTTGATA AAAAGACGGA GAAATTTTGT CAATCGCAGG AGCGTTTCCT CAATATGTCA 
ACTAAAAAGC CCGAAAACAC AATACAGGAG GCTGACGCCA GTTTGGGGAT GCATGAACGC 
GAGTATATCC AGGAGTCGCT GAGCTATGTG CTGCGCATCC AAGAAGTGCA GGAACGAATC 
AAATTCGAAT TTGTCGAAAT ATTGCTAGCA TTTATCTCTG GCTGGCTGGT TTTCTACCAC 
ACGGCGCACG AACAGGCGGA GGATCATCGT GATTATCTGC AGGATTTGAG GCACAAAGTT 
CAAAAAACTC GGGAAAATTT CGAGGAGGCA CGCGAAAAAG TGACTGAATT GAAAACAAAA 
TATATGGAAA AACGCACAAA GCCCGAGGAA ATTTTTACAA AACGTGGCTA CCTATTTCTA 
ATGGAGAAAA AGCCATTCAA AGCCACCTGG ACAAAATACT ATTGTACATT CAAGAAGCAA 
AAGCGCGAGT TCACTATGCT CCAGTTCAAC CAGATGAATC ACAACTTCAC GAGACCCGAG 
GCGCGTGACG ACGAGAAACT GACGCTTTTC TCCTGCCAGC GACGGGCATC TGAGTTCGAG 
AAGAGGTTCT GCTTCGATTT GACCTTCAAG GAGAAGCCAG GCGTCGTCTA CACATTTCAG 
GCTTTGAGCG AAAAGGATCA TCGCTATTGG ATTAGTGCCA TGGATGGCAC GGAGCCGACA 
TATCTGGCTC CCGGCAAAAT CAAAGTCAGC GAGGCATACC ATCTGGATGA AGCCGGATTC 
ATGTTCATCC GCAGATGCAT TCAAGTATTA GAGATTCGAG GCCTGGAGGA TGAGGGCATA 
TACAGAAAGA GCGGTGTTGG AACCAAAATC AGCAAGCTGC TAGCACTGGG CCTCAATCAA 
AAGGAGAGCG ACGATGTCTT CGTCGACGAC AAGTACCGCG ATTTGATGGA AAGCAACACC 
ATAGCTAGTG CCCTAAAAAT GTATCTTCGA AATCTGAATG AGCCATTGAT GACGTATCAA 
TATCATAGTG ATTTCATTGA AGCCGCAAAA CAAGAAACCC TCAATCAGCG CGTGAATGAG 
GTGCACAAGC TGGTCTACAA GTTGCCGCAG CCCAATTTTC AGATGCTCGA CATGGTTATT 
TGCCATCTGA CTGACGTTTC GCGCAAGTAC GAGAAGAACA AAATGTCCGT ATTTAATCTG 
GGAGTGGTGT TTGGGCCAAC ATTGTTGCGT CCTCGAGAGG AATCAGTTGC CGCCATATTG 
GATATTAAGT TTAATAATAT CGTCATCAAC ATTCTAATTG ATAACTACGA GAGGATATTC 
AAGAACAAGC CCAGCGCCGA TATCAAGTTG CCGGACGCCA CGAAGGCACC CAGCATCATG 
TACCCCAGTC GCAGCAGTCC ACCGACCAGA ATGCCGCGAG CCTCTCAAAT CGGCAAGGCC 
GCTTCAACGG GTGCAATGGG TAGCAGCAGT AATACAGCGG GCAATCCGAT GTTCTTGAAT 
CAGCAGAAAA TCTATCGCGT TGTGAGCAAA ACCAACTGCA CCGAGCCCAC AATGTCATCC 
AGCCTGCAAA ATATTCCGAA TGGCGATAAC TATGCCCTTG GCTCGAACGC CATGAATAGC 
TCGGGCGGTG CTCAATGCAT ATCCCTGTCG CCGCCGATGC ACATGCTGAA CGGCATACTC 
TCGCCGACCA TTGGTAGCAT CAATAATCTG CACACAATAT CGAAAAATGA GACCAGCACA 
CGACGATACG TGCCCGACAC TGAGATTGCG CCCGATTATG GTATTATAGG TGCTAACAAC 
GGCGGTGTGA CGCTGCAATC GCATCACGCT GTGCCGGTTA GTAGCACGAG TAACTTTCGA 
CATCCCGAGT ACCTAATGAC CACGGCCAAT CCAGTTACTC AATCGGGCTC AAGTAGCCAT 
ATATATACGA ACACATCTAG TGCTGGTGCT AACTCAAGCA ATCGGATTAG TCTAAGCAAT 
GTATCCCCGC CAAATACTGC AATGCGCAAG GAGCGATTCC TGGGCAGCGC CAGTGGTCCG 
CAGCAGCATC CGCCCGTTCA ACGGGGTCTG CACTCGTACG GCCAGACAAA ACACTATTCA 
CCCCTAATGC CAACGTCAAC ATCCAGCTCC AACGACAGCG TATGTGATTC ACTATCTTCC 
AACAACGGCC TGGGCAGCTC CATTGTGGCC AATAGTGGCA ACAGTGGGAA TGTTGCCCAG 
CACTTGAGCG CACGGAACAC TAATGACTAT GCGCTGATAT CGTCACAGAA TTCATCGCTG 
TCGCCGCATC TTGGACCCAC CTGCGACGAG GTCGTCACGG CCACAACATT GCCCTCGGTT 
AGTTTAAGCT GTGGTGGCAC CGCCAGCGAA AGCACCGAAT ATCCGCCTAG TAAAATGCAT 
CGTAATCGTG ACGTCAACCA AATAAAACGT GATTTGTCAA CGGGCACCGC TCGTGTGCGC 
ACACTCTATG CTTGCATGGG AGAAAGCGAG GGCGAGCTTT CTTTCGAGCC CAACCAAATA 
ATAACCAATG TTCGTTACTC CCACGAACCC GGCTGGCTGC AGGGCACACT AAATGGGAAA 
ACAGGACTCA TTCCGGAGAA CTATGTGGAA CATTTGAAGC CGCACCATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996445.1
CDS	378..3428
Translation	MGGGKNVRRGLEPLEFEECIVDSPDFRENLNRHEKELDHTSHQIKRIIKEVKDLMSAAKILSTRMKQLAILLNDFNFECIGTAQTDDENVICESLKRFGAIIGNIEDEREKMLTLADKHIIESLEDFRKKQIGGVKENKKKFDKKTEKFCQSQERFLNMSTKKPENTIQEADASLGMHEREYIQESLSYVLRIQEVQERIKFEFVEILLAFISGWLVFYHTAHEQAEDHRDYLQDLRHKVQKTRENFEEAREKVTELKTKYMEKRTKPEEIFTKRGYLFLMEKKPFKATWTKYYCTFKKQKREFTMLQFNQMNHNFTRPEARDDEKLTLFSCQRRASEFEKRFCFDLTFKEKPGVVYTFQALSEKDHRYWISAMDGTEPTYLAPGKIKVSEAYHLDEAGFMFIRRCIQVLEIRGLEDEGIYRKSGVGTKISKLLALGLNQKESDDVFVDDKYRDLMESNTIASALKMYLRNLNEPLMTYQYHSDFIEAAKQETLNQRVNEVHKLVYKLPQPNFQMLDMVICHLTDVSRKYEKNKMSVFNLGVVFGPTLLRPREESVAAILDIKFNNIVINILIDNYERIFKNKPSADIKLPDATKAPSIMYPSRSSPPTRMPRASQIGKAASTGAMGSSSNTAGNPMFLNQQKIYRVVSKTNCTEPTMSSSLQNIPNGDNYALGSNAMNSSGGAQCISLSPPMHMLNGILSPTIGSINNLHTISKNETSTRRYVPDTEIAPDYGIIGANNGGVTLQSHHAVPVSSTSNFRHPEYLMTTANPVTQSGSSSHIYTNTSSAGANSSNRISLSNVSPPNTAMRKERFLGSASGPQQHPPVQRGLHSYGQTKHYSPLMPTSTSSSNDSVCDSLSSNNGLGSSIVANSGNSGNVAQHLSARNTNDYALISSQNSSLSPHLGPTCDEVVTATTLPSVSLSCGGTASESTEYPPSKMHRNRDVNQIKRDLSTGTARVRTLYACMGESEGELSFEPNQIITNVRYSHEPGWLQGTLNGKTGLIPENYVEHLKPHH

Target ORF information:

RefSeq Version	NM_206722.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster GTPase regulator associated with FAK (Graf), transcript variant J, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206722.2

ORF Insert Sequence:

ATGGGCGGCG GCAAAAATGT ACGCCGCGGC TTGGAGCCTC TGGAGTTCGA AGAATGCATC 
GTCGACAGTC CGGACTTTCG CGAAAATTTA AATCGACATG AAAAGGAACT GGACCACACT 
AGTCATCAAA TAAAACGAAT TATCAAAGAG GTCAAAGATC TTATGAGTGC GGCAAAGATC 
CTGTCCACTC GAATGAAACA ATTGGCCATT TTACTTAACG ATTTTAACTT TGAGTGCATT 
GGCACTGCTC AGACCGACGA TGAGAATGTC ATTTGTGAAA GTCTCAAGCG ATTTGGTGCT 
ATAATTGGCA ATATTGAAGA TGAGCGCGAA AAAATGCTAA CGCTCGCCGA TAAACATATT 
ATTGAGTCAC TGGAGGATTT TCGAAAAAAG CAAATCGGTG GCGTCAAAGA AAACAAAAAG 
AAATTTGATA AAAAGACGGA GAAATTTTGT CAATCGCAGG AGCGTTTCCT CAATATGTCA 
ACTAAAAAGC CCGAAAACAC AATACAGGAG GCTGACGCCA GTTTGGGGAT GCATGAACGC 
GAGTATATCC AGGAGTCGCT GAGCTATGTG CTGCGCATCC AAGAAGTGCA GGAACGAATC 
AAATTCGAAT TTGTCGAAAT ATTGCTAGCA TTTATCTCTG GCTGGCTGGT TTTCTACCAC 
ACGGCGCACG AACAGGCGGA GGATCATCGT GATTATCTGC AGGATTTGAG GCACAAAGTT 
CAAAAAACTC GGGAAAATTT CGAGGAGGCA CGCGAAAAAG TGACTGAATT GAAAACAAAA 
TATATGGAAA AACGCACAAA GCCCGAGGAA ATTTTTACAA AACGTGGCTA CCTATTTCTA 
ATGGAGAAAA AGCCATTCAA AGCCACCTGG ACAAAATACT ATTGTACATT CAAGAAGCAA 
AAGCGCGAGT TCACTATGCT CCAGTTCAAC CAGATGAATC ACAACTTCAC GAGACCCGAG 
GCGCGTGACG ACGAGAAACT GACGCTTTTC TCCTGCCAGC GACGGGCATC TGAGTTCGAG 
AAGAGGTTCT GCTTCGATTT GACCTTCAAG GAGAAGCCAG GCGTCGTCTA CACATTTCAG 
GCTTTGAGCG AAAAGGATCA TCGCTATTGG ATTAGTGCCA TGGATGGCAC GGAGCCGACA 
TATCTGGCTC CCGGCAAAAT CAAAGTCAGC GAGGCATACC ATCTGGATGA AGCCGGATTC 
ATGTTCATCC GCAGATGCAT TCAAGTATTA GAGATTCGAG GCCTGGAGGA TGAGGGCATA 
TACAGAAAGA GCGGTGTTGG AACCAAAATC AGCAAGCTGC TAGCACTGGG CCTCAATCAA 
AAGGAGAGCG ACGATGTCTT CGTCGACGAC AAGTACCGCG ATTTGATGGA AAGCAACACC 
ATAGCTAGTG CCCTAAAAAT GTATCTTCGA AATCTGAATG AGCCATTGAT GACGTATCAA 
TATCATAGTG ATTTCATTGA AGCCGCAAAA CAAGAAACCC TCAATCAGCG CGTGAATGAG 
GTGCACAAGC TGGTCTACAA GTTGCCGCAG CCCAATTTTC AGATGCTCGA CATGGTTATT 
TGCCATCTGA CTGACGTTTC GCGCAAGTAC GAGAAGAACA AAATGTCCGT ATTTAATCTG 
GGAGTGGTGT TTGGGCCAAC ATTGTTGCGT CCTCGAGAGG AATCAGTTGC CGCCATATTG 
GATATTAAGT TTAATAATAT CGTCATCAAC ATTCTAATTG ATAACTACGA GAGGATATTC 
AAGAACAAGC CCAGCGCCGA TATCAAGTTG CCGGACGCCA CGAAGGCACC CAGCATCATG 
TACCCCAGTC GCAGCAGTCC ACCGACCAGA ATGCCGCGAG CCTCTCAAAT CGGCAAGGCC 
GCTTCAACGG GTGCAATGGG TAGCAGCAGT AATACAGCGG GCAATCCGAT GTTCTTGAAT 
CAGCAGAAAA TCTATCGCGT TGTGAGCAAA ACCAACTGCA CCGAGCCCAC AATGTCATCC 
AGCCTGCAAA ATATTCCGAA TGGCGATAAC TATGCCCTTG GCTCGAACGC CATGAATAGC 
TCGGGCGGTG CTCAATGCAT ATCCCTGTCG CCGCCGATGC ACATGCTGAA CGGCATACTC 
TCGCCGACCA TTGGTAGCAT CAATAATCTG CACACAATAT CGAAAAATGA GACCAGCACA 
CGACGATACG TGCCCGACAC TGAGATTGCG CCCGATTATG GTATTATAGG TGCTAACAAC 
GGCGGTGTGA CGCTGCAATC GCATCACGCT GTGCCGGTTA GTAGCACGAG TAACTTTCGA 
CATCCCGAGT ACCTAATGAC CACGGCCAAT CCAGTTACTC AATCGGGCTC AAGTAGCCAT 
ATATATACGA ACACATCTAG TGCTGGTGCT AACTCAAGCA ATCGGATTAG TCTAAGCAAT 
GTATCCCCGC CAAATACTGC AATGCGCAAG GAGCGATTCC TGGGCAGCGC CAGTGGTCCG 
CAGCAGCATC CGCCCGTTCA ACGGGGTCTG CACTCGTACG GCCAGACAAA ACACTATTCA 
CCCCTAATGC CAACGTCAAC ATCCAGCTCC AACGACAGCG TATGTGATTC ACTATCTTCC 
AACAACGGCC TGGGCAGCTC CATTGTGGCC AATAGTGGCA ACAGTGGGAA TGTTGCCCAG 
CACTTGAGCG CACGGAACAC TAATGACTAT GCGCTGATAT CGTCACAGAA TTCATCGCTG 
TCGCCGCATC TTGGACCCAC CTGCGACGAG GTCGTCACGG CCACAACATT GCCCTCGGTT 
AGTTTAAGCT GTGGTGGCAC CGCCAGCGAA AGCACCGAAT ATCCGCCTAG TAAAATGCAT 
CGTAATCGTG ACGTCAACCA AATAAAACGT GATTTGTCAA CGGGCACCGC TCGTGTGCGC 
ACACTCTATG CTTGCATGGG AGAAAGCGAG GGCGAGCTTT CTTTCGAGCC CAACCAAATA 
ATAACCAATG TTCGTTACTC CCACGAACCC GGCTGGCTGC AGGGCACACT AAATGGGAAA 
ACAGGACTCA TTCCGGAGAA CTATGTGGAA CATTTGAAGC CGCACCATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Graf ( NM_206720.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996443.1 Drosophila melanogaster GTPase regulator associated with FAK (Graf), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa03599
Clone ID Related Accession (Same CDS sequence)	NM_206719.2 , NM_206722.2 , NM_206720.2 , NM_132842.3
Accession Version	NM_206720.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3051bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	GTPase regulator associated with FAK, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_206720.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCGGCG GCAAAAATGT ACGCCGCGGC TTGGAGCCTC TGGAGTTCGA AGAATGCATC 
GTCGACAGTC CGGACTTTCG CGAAAATTTA AATCGACATG AAAAGGAACT GGACCACACT 
AGTCATCAAA TAAAACGAAT TATCAAAGAG GTCAAAGATC TTATGAGTGC GGCAAAGATC 
CTGTCCACTC GAATGAAACA ATTGGCCATT TTACTTAACG ATTTTAACTT TGAGTGCATT 
GGCACTGCTC AGACCGACGA TGAGAATGTC ATTTGTGAAA GTCTCAAGCG ATTTGGTGCT 
ATAATTGGCA ATATTGAAGA TGAGCGCGAA AAAATGCTAA CGCTCGCCGA TAAACATATT 
ATTGAGTCAC TGGAGGATTT TCGAAAAAAG CAAATCGGTG GCGTCAAAGA AAACAAAAAG 
AAATTTGATA AAAAGACGGA GAAATTTTGT CAATCGCAGG AGCGTTTCCT CAATATGTCA 
ACTAAAAAGC CCGAAAACAC AATACAGGAG GCTGACGCCA GTTTGGGGAT GCATGAACGC 
GAGTATATCC AGGAGTCGCT GAGCTATGTG CTGCGCATCC AAGAAGTGCA GGAACGAATC 
AAATTCGAAT TTGTCGAAAT ATTGCTAGCA TTTATCTCTG GCTGGCTGGT TTTCTACCAC 
ACGGCGCACG AACAGGCGGA GGATCATCGT GATTATCTGC AGGATTTGAG GCACAAAGTT 
CAAAAAACTC GGGAAAATTT CGAGGAGGCA CGCGAAAAAG TGACTGAATT GAAAACAAAA 
TATATGGAAA AACGCACAAA GCCCGAGGAA ATTTTTACAA AACGTGGCTA CCTATTTCTA 
ATGGAGAAAA AGCCATTCAA AGCCACCTGG ACAAAATACT ATTGTACATT CAAGAAGCAA 
AAGCGCGAGT TCACTATGCT CCAGTTCAAC CAGATGAATC ACAACTTCAC GAGACCCGAG 
GCGCGTGACG ACGAGAAACT GACGCTTTTC TCCTGCCAGC GACGGGCATC TGAGTTCGAG 
AAGAGGTTCT GCTTCGATTT GACCTTCAAG GAGAAGCCAG GCGTCGTCTA CACATTTCAG 
GCTTTGAGCG AAAAGGATCA TCGCTATTGG ATTAGTGCCA TGGATGGCAC GGAGCCGACA 
TATCTGGCTC CCGGCAAAAT CAAAGTCAGC GAGGCATACC ATCTGGATGA AGCCGGATTC 
ATGTTCATCC GCAGATGCAT TCAAGTATTA GAGATTCGAG GCCTGGAGGA TGAGGGCATA 
TACAGAAAGA GCGGTGTTGG AACCAAAATC AGCAAGCTGC TAGCACTGGG CCTCAATCAA 
AAGGAGAGCG ACGATGTCTT CGTCGACGAC AAGTACCGCG ATTTGATGGA AAGCAACACC 
ATAGCTAGTG CCCTAAAAAT GTATCTTCGA AATCTGAATG AGCCATTGAT GACGTATCAA 
TATCATAGTG ATTTCATTGA AGCCGCAAAA CAAGAAACCC TCAATCAGCG CGTGAATGAG 
GTGCACAAGC TGGTCTACAA GTTGCCGCAG CCCAATTTTC AGATGCTCGA CATGGTTATT 
TGCCATCTGA CTGACGTTTC GCGCAAGTAC GAGAAGAACA AAATGTCCGT ATTTAATCTG 
GGAGTGGTGT TTGGGCCAAC ATTGTTGCGT CCTCGAGAGG AATCAGTTGC CGCCATATTG 
GATATTAAGT TTAATAATAT CGTCATCAAC ATTCTAATTG ATAACTACGA GAGGATATTC 
AAGAACAAGC CCAGCGCCGA TATCAAGTTG CCGGACGCCA CGAAGGCACC CAGCATCATG 
TACCCCAGTC GCAGCAGTCC ACCGACCAGA ATGCCGCGAG CCTCTCAAAT CGGCAAGGCC 
GCTTCAACGG GTGCAATGGG TAGCAGCAGT AATACAGCGG GCAATCCGAT GTTCTTGAAT 
CAGCAGAAAA TCTATCGCGT TGTGAGCAAA ACCAACTGCA CCGAGCCCAC AATGTCATCC 
AGCCTGCAAA ATATTCCGAA TGGCGATAAC TATGCCCTTG GCTCGAACGC CATGAATAGC 
TCGGGCGGTG CTCAATGCAT ATCCCTGTCG CCGCCGATGC ACATGCTGAA CGGCATACTC 
TCGCCGACCA TTGGTAGCAT CAATAATCTG CACACAATAT CGAAAAATGA GACCAGCACA 
CGACGATACG TGCCCGACAC TGAGATTGCG CCCGATTATG GTATTATAGG TGCTAACAAC 
GGCGGTGTGA CGCTGCAATC GCATCACGCT GTGCCGGTTA GTAGCACGAG TAACTTTCGA 
CATCCCGAGT ACCTAATGAC CACGGCCAAT CCAGTTACTC AATCGGGCTC AAGTAGCCAT 
ATATATACGA ACACATCTAG TGCTGGTGCT AACTCAAGCA ATCGGATTAG TCTAAGCAAT 
GTATCCCCGC CAAATACTGC AATGCGCAAG GAGCGATTCC TGGGCAGCGC CAGTGGTCCG 
CAGCAGCATC CGCCCGTTCA ACGGGGTCTG CACTCGTACG GCCAGACAAA ACACTATTCA 
CCCCTAATGC CAACGTCAAC ATCCAGCTCC AACGACAGCG TATGTGATTC ACTATCTTCC 
AACAACGGCC TGGGCAGCTC CATTGTGGCC AATAGTGGCA ACAGTGGGAA TGTTGCCCAG 
CACTTGAGCG CACGGAACAC TAATGACTAT GCGCTGATAT CGTCACAGAA TTCATCGCTG 
TCGCCGCATC TTGGACCCAC CTGCGACGAG GTCGTCACGG CCACAACATT GCCCTCGGTT 
AGTTTAAGCT GTGGTGGCAC CGCCAGCGAA AGCACCGAAT ATCCGCCTAG TAAAATGCAT 
CGTAATCGTG ACGTCAACCA AATAAAACGT GATTTGTCAA CGGGCACCGC TCGTGTGCGC 
ACACTCTATG CTTGCATGGG AGAAAGCGAG GGCGAGCTTT CTTTCGAGCC CAACCAAATA 
ATAACCAATG TTCGTTACTC CCACGAACCC GGCTGGCTGC AGGGCACACT AAATGGGAAA 
ACAGGACTCA TTCCGGAGAA CTATGTGGAA CATTTGAAGC CGCACCATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996443.1
CDS	753..3803
Translation	MGGGKNVRRGLEPLEFEECIVDSPDFRENLNRHEKELDHTSHQIKRIIKEVKDLMSAAKILSTRMKQLAILLNDFNFECIGTAQTDDENVICESLKRFGAIIGNIEDEREKMLTLADKHIIESLEDFRKKQIGGVKENKKKFDKKTEKFCQSQERFLNMSTKKPENTIQEADASLGMHEREYIQESLSYVLRIQEVQERIKFEFVEILLAFISGWLVFYHTAHEQAEDHRDYLQDLRHKVQKTRENFEEAREKVTELKTKYMEKRTKPEEIFTKRGYLFLMEKKPFKATWTKYYCTFKKQKREFTMLQFNQMNHNFTRPEARDDEKLTLFSCQRRASEFEKRFCFDLTFKEKPGVVYTFQALSEKDHRYWISAMDGTEPTYLAPGKIKVSEAYHLDEAGFMFIRRCIQVLEIRGLEDEGIYRKSGVGTKISKLLALGLNQKESDDVFVDDKYRDLMESNTIASALKMYLRNLNEPLMTYQYHSDFIEAAKQETLNQRVNEVHKLVYKLPQPNFQMLDMVICHLTDVSRKYEKNKMSVFNLGVVFGPTLLRPREESVAAILDIKFNNIVINILIDNYERIFKNKPSADIKLPDATKAPSIMYPSRSSPPTRMPRASQIGKAASTGAMGSSSNTAGNPMFLNQQKIYRVVSKTNCTEPTMSSSLQNIPNGDNYALGSNAMNSSGGAQCISLSPPMHMLNGILSPTIGSINNLHTISKNETSTRRYVPDTEIAPDYGIIGANNGGVTLQSHHAVPVSSTSNFRHPEYLMTTANPVTQSGSSSHIYTNTSSAGANSSNRISLSNVSPPNTAMRKERFLGSASGPQQHPPVQRGLHSYGQTKHYSPLMPTSTSSSNDSVCDSLSSNNGLGSSIVANSGNSGNVAQHLSARNTNDYALISSQNSSLSPHLGPTCDEVVTATTLPSVSLSCGGTASESTEYPPSKMHRNRDVNQIKRDLSTGTARVRTLYACMGESEGELSFEPNQIITNVRYSHEPGWLQGTLNGKTGLIPENYVEHLKPHH

Target ORF information:

RefSeq Version	NM_206720.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster GTPase regulator associated with FAK (Graf), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206720.2

ORF Insert Sequence:

ATGGGCGGCG GCAAAAATGT ACGCCGCGGC TTGGAGCCTC TGGAGTTCGA AGAATGCATC 
GTCGACAGTC CGGACTTTCG CGAAAATTTA AATCGACATG AAAAGGAACT GGACCACACT 
AGTCATCAAA TAAAACGAAT TATCAAAGAG GTCAAAGATC TTATGAGTGC GGCAAAGATC 
CTGTCCACTC GAATGAAACA ATTGGCCATT TTACTTAACG ATTTTAACTT TGAGTGCATT 
GGCACTGCTC AGACCGACGA TGAGAATGTC ATTTGTGAAA GTCTCAAGCG ATTTGGTGCT 
ATAATTGGCA ATATTGAAGA TGAGCGCGAA AAAATGCTAA CGCTCGCCGA TAAACATATT 
ATTGAGTCAC TGGAGGATTT TCGAAAAAAG CAAATCGGTG GCGTCAAAGA AAACAAAAAG 
AAATTTGATA AAAAGACGGA GAAATTTTGT CAATCGCAGG AGCGTTTCCT CAATATGTCA 
ACTAAAAAGC CCGAAAACAC AATACAGGAG GCTGACGCCA GTTTGGGGAT GCATGAACGC 
GAGTATATCC AGGAGTCGCT GAGCTATGTG CTGCGCATCC AAGAAGTGCA GGAACGAATC 
AAATTCGAAT TTGTCGAAAT ATTGCTAGCA TTTATCTCTG GCTGGCTGGT TTTCTACCAC 
ACGGCGCACG AACAGGCGGA GGATCATCGT GATTATCTGC AGGATTTGAG GCACAAAGTT 
CAAAAAACTC GGGAAAATTT CGAGGAGGCA CGCGAAAAAG TGACTGAATT GAAAACAAAA 
TATATGGAAA AACGCACAAA GCCCGAGGAA ATTTTTACAA AACGTGGCTA CCTATTTCTA 
ATGGAGAAAA AGCCATTCAA AGCCACCTGG ACAAAATACT ATTGTACATT CAAGAAGCAA 
AAGCGCGAGT TCACTATGCT CCAGTTCAAC CAGATGAATC ACAACTTCAC GAGACCCGAG 
GCGCGTGACG ACGAGAAACT GACGCTTTTC TCCTGCCAGC GACGGGCATC TGAGTTCGAG 
AAGAGGTTCT GCTTCGATTT GACCTTCAAG GAGAAGCCAG GCGTCGTCTA CACATTTCAG 
GCTTTGAGCG AAAAGGATCA TCGCTATTGG ATTAGTGCCA TGGATGGCAC GGAGCCGACA 
TATCTGGCTC CCGGCAAAAT CAAAGTCAGC GAGGCATACC ATCTGGATGA AGCCGGATTC 
ATGTTCATCC GCAGATGCAT TCAAGTATTA GAGATTCGAG GCCTGGAGGA TGAGGGCATA 
TACAGAAAGA GCGGTGTTGG AACCAAAATC AGCAAGCTGC TAGCACTGGG CCTCAATCAA 
AAGGAGAGCG ACGATGTCTT CGTCGACGAC AAGTACCGCG ATTTGATGGA AAGCAACACC 
ATAGCTAGTG CCCTAAAAAT GTATCTTCGA AATCTGAATG AGCCATTGAT GACGTATCAA 
TATCATAGTG ATTTCATTGA AGCCGCAAAA CAAGAAACCC TCAATCAGCG CGTGAATGAG 
GTGCACAAGC TGGTCTACAA GTTGCCGCAG CCCAATTTTC AGATGCTCGA CATGGTTATT 
TGCCATCTGA CTGACGTTTC GCGCAAGTAC GAGAAGAACA AAATGTCCGT ATTTAATCTG 
GGAGTGGTGT TTGGGCCAAC ATTGTTGCGT CCTCGAGAGG AATCAGTTGC CGCCATATTG 
GATATTAAGT TTAATAATAT CGTCATCAAC ATTCTAATTG ATAACTACGA GAGGATATTC 
AAGAACAAGC CCAGCGCCGA TATCAAGTTG CCGGACGCCA CGAAGGCACC CAGCATCATG 
TACCCCAGTC GCAGCAGTCC ACCGACCAGA ATGCCGCGAG CCTCTCAAAT CGGCAAGGCC 
GCTTCAACGG GTGCAATGGG TAGCAGCAGT AATACAGCGG GCAATCCGAT GTTCTTGAAT 
CAGCAGAAAA TCTATCGCGT TGTGAGCAAA ACCAACTGCA CCGAGCCCAC AATGTCATCC 
AGCCTGCAAA ATATTCCGAA TGGCGATAAC TATGCCCTTG GCTCGAACGC CATGAATAGC 
TCGGGCGGTG CTCAATGCAT ATCCCTGTCG CCGCCGATGC ACATGCTGAA CGGCATACTC 
TCGCCGACCA TTGGTAGCAT CAATAATCTG CACACAATAT CGAAAAATGA GACCAGCACA 
CGACGATACG TGCCCGACAC TGAGATTGCG CCCGATTATG GTATTATAGG TGCTAACAAC 
GGCGGTGTGA CGCTGCAATC GCATCACGCT GTGCCGGTTA GTAGCACGAG TAACTTTCGA 
CATCCCGAGT ACCTAATGAC CACGGCCAAT CCAGTTACTC AATCGGGCTC AAGTAGCCAT 
ATATATACGA ACACATCTAG TGCTGGTGCT AACTCAAGCA ATCGGATTAG TCTAAGCAAT 
GTATCCCCGC CAAATACTGC AATGCGCAAG GAGCGATTCC TGGGCAGCGC CAGTGGTCCG 
CAGCAGCATC CGCCCGTTCA ACGGGGTCTG CACTCGTACG GCCAGACAAA ACACTATTCA 
CCCCTAATGC CAACGTCAAC ATCCAGCTCC AACGACAGCG TATGTGATTC ACTATCTTCC 
AACAACGGCC TGGGCAGCTC CATTGTGGCC AATAGTGGCA ACAGTGGGAA TGTTGCCCAG 
CACTTGAGCG CACGGAACAC TAATGACTAT GCGCTGATAT CGTCACAGAA TTCATCGCTG 
TCGCCGCATC TTGGACCCAC CTGCGACGAG GTCGTCACGG CCACAACATT GCCCTCGGTT 
AGTTTAAGCT GTGGTGGCAC CGCCAGCGAA AGCACCGAAT ATCCGCCTAG TAAAATGCAT 
CGTAATCGTG ACGTCAACCA AATAAAACGT GATTTGTCAA CGGGCACCGC TCGTGTGCGC 
ACACTCTATG CTTGCATGGG AGAAAGCGAG GGCGAGCTTT CTTTCGAGCC CAACCAAATA 
ATAACCAATG TTCGTTACTC CCACGAACCC GGCTGGCTGC AGGGCACACT AAATGGGAAA 
ACAGGACTCA TTCCGGAGAA CTATGTGGAA CATTTGAAGC CGCACCATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Graf ( NM_132842.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_573070.2 Drosophila melanogaster GTPase regulator associated with FAK (Graf), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa03599
Clone ID Related Accession (Same CDS sequence)	NM_206719.2 , NM_206722.2 , NM_206720.2 , NM_132842.3
Accession Version	NM_132842.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3051bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	GTPase regulator associated with FAK, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_132842.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCGGCG GCAAAAATGT ACGCCGCGGC TTGGAGCCTC TGGAGTTCGA AGAATGCATC 
GTCGACAGTC CGGACTTTCG CGAAAATTTA AATCGACATG AAAAGGAACT GGACCACACT 
AGTCATCAAA TAAAACGAAT TATCAAAGAG GTCAAAGATC TTATGAGTGC GGCAAAGATC 
CTGTCCACTC GAATGAAACA ATTGGCCATT TTACTTAACG ATTTTAACTT TGAGTGCATT 
GGCACTGCTC AGACCGACGA TGAGAATGTC ATTTGTGAAA GTCTCAAGCG ATTTGGTGCT 
ATAATTGGCA ATATTGAAGA TGAGCGCGAA AAAATGCTAA CGCTCGCCGA TAAACATATT 
ATTGAGTCAC TGGAGGATTT TCGAAAAAAG CAAATCGGTG GCGTCAAAGA AAACAAAAAG 
AAATTTGATA AAAAGACGGA GAAATTTTGT CAATCGCAGG AGCGTTTCCT CAATATGTCA 
ACTAAAAAGC CCGAAAACAC AATACAGGAG GCTGACGCCA GTTTGGGGAT GCATGAACGC 
GAGTATATCC AGGAGTCGCT GAGCTATGTG CTGCGCATCC AAGAAGTGCA GGAACGAATC 
AAATTCGAAT TTGTCGAAAT ATTGCTAGCA TTTATCTCTG GCTGGCTGGT TTTCTACCAC 
ACGGCGCACG AACAGGCGGA GGATCATCGT GATTATCTGC AGGATTTGAG GCACAAAGTT 
CAAAAAACTC GGGAAAATTT CGAGGAGGCA CGCGAAAAAG TGACTGAATT GAAAACAAAA 
TATATGGAAA AACGCACAAA GCCCGAGGAA ATTTTTACAA AACGTGGCTA CCTATTTCTA 
ATGGAGAAAA AGCCATTCAA AGCCACCTGG ACAAAATACT ATTGTACATT CAAGAAGCAA 
AAGCGCGAGT TCACTATGCT CCAGTTCAAC CAGATGAATC ACAACTTCAC GAGACCCGAG 
GCGCGTGACG ACGAGAAACT GACGCTTTTC TCCTGCCAGC GACGGGCATC TGAGTTCGAG 
AAGAGGTTCT GCTTCGATTT GACCTTCAAG GAGAAGCCAG GCGTCGTCTA CACATTTCAG 
GCTTTGAGCG AAAAGGATCA TCGCTATTGG ATTAGTGCCA TGGATGGCAC GGAGCCGACA 
TATCTGGCTC CCGGCAAAAT CAAAGTCAGC GAGGCATACC ATCTGGATGA AGCCGGATTC 
ATGTTCATCC GCAGATGCAT TCAAGTATTA GAGATTCGAG GCCTGGAGGA TGAGGGCATA 
TACAGAAAGA GCGGTGTTGG AACCAAAATC AGCAAGCTGC TAGCACTGGG CCTCAATCAA 
AAGGAGAGCG ACGATGTCTT CGTCGACGAC AAGTACCGCG ATTTGATGGA AAGCAACACC 
ATAGCTAGTG CCCTAAAAAT GTATCTTCGA AATCTGAATG AGCCATTGAT GACGTATCAA 
TATCATAGTG ATTTCATTGA AGCCGCAAAA CAAGAAACCC TCAATCAGCG CGTGAATGAG 
GTGCACAAGC TGGTCTACAA GTTGCCGCAG CCCAATTTTC AGATGCTCGA CATGGTTATT 
TGCCATCTGA CTGACGTTTC GCGCAAGTAC GAGAAGAACA AAATGTCCGT ATTTAATCTG 
GGAGTGGTGT TTGGGCCAAC ATTGTTGCGT CCTCGAGAGG AATCAGTTGC CGCCATATTG 
GATATTAAGT TTAATAATAT CGTCATCAAC ATTCTAATTG ATAACTACGA GAGGATATTC 
AAGAACAAGC CCAGCGCCGA TATCAAGTTG CCGGACGCCA CGAAGGCACC CAGCATCATG 
TACCCCAGTC GCAGCAGTCC ACCGACCAGA ATGCCGCGAG CCTCTCAAAT CGGCAAGGCC 
GCTTCAACGG GTGCAATGGG TAGCAGCAGT AATACAGCGG GCAATCCGAT GTTCTTGAAT 
CAGCAGAAAA TCTATCGCGT TGTGAGCAAA ACCAACTGCA CCGAGCCCAC AATGTCATCC 
AGCCTGCAAA ATATTCCGAA TGGCGATAAC TATGCCCTTG GCTCGAACGC CATGAATAGC 
TCGGGCGGTG CTCAATGCAT ATCCCTGTCG CCGCCGATGC ACATGCTGAA CGGCATACTC 
TCGCCGACCA TTGGTAGCAT CAATAATCTG CACACAATAT CGAAAAATGA GACCAGCACA 
CGACGATACG TGCCCGACAC TGAGATTGCG CCCGATTATG GTATTATAGG TGCTAACAAC 
GGCGGTGTGA CGCTGCAATC GCATCACGCT GTGCCGGTTA GTAGCACGAG TAACTTTCGA 
CATCCCGAGT ACCTAATGAC CACGGCCAAT CCAGTTACTC AATCGGGCTC AAGTAGCCAT 
ATATATACGA ACACATCTAG TGCTGGTGCT AACTCAAGCA ATCGGATTAG TCTAAGCAAT 
GTATCCCCGC CAAATACTGC AATGCGCAAG GAGCGATTCC TGGGCAGCGC CAGTGGTCCG 
CAGCAGCATC CGCCCGTTCA ACGGGGTCTG CACTCGTACG GCCAGACAAA ACACTATTCA 
CCCCTAATGC CAACGTCAAC ATCCAGCTCC AACGACAGCG TATGTGATTC ACTATCTTCC 
AACAACGGCC TGGGCAGCTC CATTGTGGCC AATAGTGGCA ACAGTGGGAA TGTTGCCCAG 
CACTTGAGCG CACGGAACAC TAATGACTAT GCGCTGATAT CGTCACAGAA TTCATCGCTG 
TCGCCGCATC TTGGACCCAC CTGCGACGAG GTCGTCACGG CCACAACATT GCCCTCGGTT 
AGTTTAAGCT GTGGTGGCAC CGCCAGCGAA AGCACCGAAT ATCCGCCTAG TAAAATGCAT 
CGTAATCGTG ACGTCAACCA AATAAAACGT GATTTGTCAA CGGGCACCGC TCGTGTGCGC 
ACACTCTATG CTTGCATGGG AGAAAGCGAG GGCGAGCTTT CTTTCGAGCC CAACCAAATA 
ATAACCAATG TTCGTTACTC CCACGAACCC GGCTGGCTGC AGGGCACACT AAATGGGAAA 
ACAGGACTCA TTCCGGAGAA CTATGTGGAA CATTTGAAGC CGCACCATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_573070.2
CDS	468..3518
Translation	MGGGKNVRRGLEPLEFEECIVDSPDFRENLNRHEKELDHTSHQIKRIIKEVKDLMSAAKILSTRMKQLAILLNDFNFECIGTAQTDDENVICESLKRFGAIIGNIEDEREKMLTLADKHIIESLEDFRKKQIGGVKENKKKFDKKTEKFCQSQERFLNMSTKKPENTIQEADASLGMHEREYIQESLSYVLRIQEVQERIKFEFVEILLAFISGWLVFYHTAHEQAEDHRDYLQDLRHKVQKTRENFEEAREKVTELKTKYMEKRTKPEEIFTKRGYLFLMEKKPFKATWTKYYCTFKKQKREFTMLQFNQMNHNFTRPEARDDEKLTLFSCQRRASEFEKRFCFDLTFKEKPGVVYTFQALSEKDHRYWISAMDGTEPTYLAPGKIKVSEAYHLDEAGFMFIRRCIQVLEIRGLEDEGIYRKSGVGTKISKLLALGLNQKESDDVFVDDKYRDLMESNTIASALKMYLRNLNEPLMTYQYHSDFIEAAKQETLNQRVNEVHKLVYKLPQPNFQMLDMVICHLTDVSRKYEKNKMSVFNLGVVFGPTLLRPREESVAAILDIKFNNIVINILIDNYERIFKNKPSADIKLPDATKAPSIMYPSRSSPPTRMPRASQIGKAASTGAMGSSSNTAGNPMFLNQQKIYRVVSKTNCTEPTMSSSLQNIPNGDNYALGSNAMNSSGGAQCISLSPPMHMLNGILSPTIGSINNLHTISKNETSTRRYVPDTEIAPDYGIIGANNGGVTLQSHHAVPVSSTSNFRHPEYLMTTANPVTQSGSSSHIYTNTSSAGANSSNRISLSNVSPPNTAMRKERFLGSASGPQQHPPVQRGLHSYGQTKHYSPLMPTSTSSSNDSVCDSLSSNNGLGSSIVANSGNSGNVAQHLSARNTNDYALISSQNSSLSPHLGPTCDEVVTATTLPSVSLSCGGTASESTEYPPSKMHRNRDVNQIKRDLSTGTARVRTLYACMGESEGELSFEPNQIITNVRYSHEPGWLQGTLNGKTGLIPENYVEHLKPHH

Target ORF information:

RefSeq Version	NM_132842.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster GTPase regulator associated with FAK (Graf), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132842.3

ORF Insert Sequence:

ATGGGCGGCG GCAAAAATGT ACGCCGCGGC TTGGAGCCTC TGGAGTTCGA AGAATGCATC 
GTCGACAGTC CGGACTTTCG CGAAAATTTA AATCGACATG AAAAGGAACT GGACCACACT 
AGTCATCAAA TAAAACGAAT TATCAAAGAG GTCAAAGATC TTATGAGTGC GGCAAAGATC 
CTGTCCACTC GAATGAAACA ATTGGCCATT TTACTTAACG ATTTTAACTT TGAGTGCATT 
GGCACTGCTC AGACCGACGA TGAGAATGTC ATTTGTGAAA GTCTCAAGCG ATTTGGTGCT 
ATAATTGGCA ATATTGAAGA TGAGCGCGAA AAAATGCTAA CGCTCGCCGA TAAACATATT 
ATTGAGTCAC TGGAGGATTT TCGAAAAAAG CAAATCGGTG GCGTCAAAGA AAACAAAAAG 
AAATTTGATA AAAAGACGGA GAAATTTTGT CAATCGCAGG AGCGTTTCCT CAATATGTCA 
ACTAAAAAGC CCGAAAACAC AATACAGGAG GCTGACGCCA GTTTGGGGAT GCATGAACGC 
GAGTATATCC AGGAGTCGCT GAGCTATGTG CTGCGCATCC AAGAAGTGCA GGAACGAATC 
AAATTCGAAT TTGTCGAAAT ATTGCTAGCA TTTATCTCTG GCTGGCTGGT TTTCTACCAC 
ACGGCGCACG AACAGGCGGA GGATCATCGT GATTATCTGC AGGATTTGAG GCACAAAGTT 
CAAAAAACTC GGGAAAATTT CGAGGAGGCA CGCGAAAAAG TGACTGAATT GAAAACAAAA 
TATATGGAAA AACGCACAAA GCCCGAGGAA ATTTTTACAA AACGTGGCTA CCTATTTCTA 
ATGGAGAAAA AGCCATTCAA AGCCACCTGG ACAAAATACT ATTGTACATT CAAGAAGCAA 
AAGCGCGAGT TCACTATGCT CCAGTTCAAC CAGATGAATC ACAACTTCAC GAGACCCGAG 
GCGCGTGACG ACGAGAAACT GACGCTTTTC TCCTGCCAGC GACGGGCATC TGAGTTCGAG 
AAGAGGTTCT GCTTCGATTT GACCTTCAAG GAGAAGCCAG GCGTCGTCTA CACATTTCAG 
GCTTTGAGCG AAAAGGATCA TCGCTATTGG ATTAGTGCCA TGGATGGCAC GGAGCCGACA 
TATCTGGCTC CCGGCAAAAT CAAAGTCAGC GAGGCATACC ATCTGGATGA AGCCGGATTC 
ATGTTCATCC GCAGATGCAT TCAAGTATTA GAGATTCGAG GCCTGGAGGA TGAGGGCATA 
TACAGAAAGA GCGGTGTTGG AACCAAAATC AGCAAGCTGC TAGCACTGGG CCTCAATCAA 
AAGGAGAGCG ACGATGTCTT CGTCGACGAC AAGTACCGCG ATTTGATGGA AAGCAACACC 
ATAGCTAGTG CCCTAAAAAT GTATCTTCGA AATCTGAATG AGCCATTGAT GACGTATCAA 
TATCATAGTG ATTTCATTGA AGCCGCAAAA CAAGAAACCC TCAATCAGCG CGTGAATGAG 
GTGCACAAGC TGGTCTACAA GTTGCCGCAG CCCAATTTTC AGATGCTCGA CATGGTTATT 
TGCCATCTGA CTGACGTTTC GCGCAAGTAC GAGAAGAACA AAATGTCCGT ATTTAATCTG 
GGAGTGGTGT TTGGGCCAAC ATTGTTGCGT CCTCGAGAGG AATCAGTTGC CGCCATATTG 
GATATTAAGT TTAATAATAT CGTCATCAAC ATTCTAATTG ATAACTACGA GAGGATATTC 
AAGAACAAGC CCAGCGCCGA TATCAAGTTG CCGGACGCCA CGAAGGCACC CAGCATCATG 
TACCCCAGTC GCAGCAGTCC ACCGACCAGA ATGCCGCGAG CCTCTCAAAT CGGCAAGGCC 
GCTTCAACGG GTGCAATGGG TAGCAGCAGT AATACAGCGG GCAATCCGAT GTTCTTGAAT 
CAGCAGAAAA TCTATCGCGT TGTGAGCAAA ACCAACTGCA CCGAGCCCAC AATGTCATCC 
AGCCTGCAAA ATATTCCGAA TGGCGATAAC TATGCCCTTG GCTCGAACGC CATGAATAGC 
TCGGGCGGTG CTCAATGCAT ATCCCTGTCG CCGCCGATGC ACATGCTGAA CGGCATACTC 
TCGCCGACCA TTGGTAGCAT CAATAATCTG CACACAATAT CGAAAAATGA GACCAGCACA 
CGACGATACG TGCCCGACAC TGAGATTGCG CCCGATTATG GTATTATAGG TGCTAACAAC 
GGCGGTGTGA CGCTGCAATC GCATCACGCT GTGCCGGTTA GTAGCACGAG TAACTTTCGA 
CATCCCGAGT ACCTAATGAC CACGGCCAAT CCAGTTACTC AATCGGGCTC AAGTAGCCAT 
ATATATACGA ACACATCTAG TGCTGGTGCT AACTCAAGCA ATCGGATTAG TCTAAGCAAT 
GTATCCCCGC CAAATACTGC AATGCGCAAG GAGCGATTCC TGGGCAGCGC CAGTGGTCCG 
CAGCAGCATC CGCCCGTTCA ACGGGGTCTG CACTCGTACG GCCAGACAAA ACACTATTCA 
CCCCTAATGC CAACGTCAAC ATCCAGCTCC AACGACAGCG TATGTGATTC ACTATCTTCC 
AACAACGGCC TGGGCAGCTC CATTGTGGCC AATAGTGGCA ACAGTGGGAA TGTTGCCCAG 
CACTTGAGCG CACGGAACAC TAATGACTAT GCGCTGATAT CGTCACAGAA TTCATCGCTG 
TCGCCGCATC TTGGACCCAC CTGCGACGAG GTCGTCACGG CCACAACATT GCCCTCGGTT 
AGTTTAAGCT GTGGTGGCAC CGCCAGCGAA AGCACCGAAT ATCCGCCTAG TAAAATGCAT 
CGTAATCGTG ACGTCAACCA AATAAAACGT GATTTGTCAA CGGGCACCGC TCGTGTGCGC 
ACACTCTATG CTTGCATGGG AGAAAGCGAG GGCGAGCTTT CTTTCGAGCC CAACCAAATA 
ATAACCAATG TTCGTTACTC CCACGAACCC GGCTGGCTGC AGGGCACACT AAATGGGAAA 
ACAGGACTCA TTCCGGAGAA CTATGTGGAA CATTTGAAGC CGCACCATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Graf ( NM_206718.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996441.2 Drosophila melanogaster GTPase regulator associated with FAK (Graf), transcript variant G, mRNA.

Price & Availability↑

CloneID	OFa21492
Clone ID Related Accession (Same CDS sequence)	NM_206718.2 , NM_206721.2 , NM_001298367.1
Accession Version	NM_206718.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3078bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	GTPase regulator associated with FAK, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_206718.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCGGCG GCAAAAATGT ACGCCGCGGC TTGGAGCCTC TGGAGTTCGA AGAATGCATC 
GTCGACAGTC CGGACTTTCG CGAAAATTTA AATCGACATG AAAAGGAACT GGACCACACT 
AGTCATCAAA TAAAACGAAT TATCAAAGAG GTCAAAGATC TTATGAGTGC GGCAAAGATC 
CTGTCCACTC GAATGAAACA ATTGGCCATT TTACTTAACG ATTTTAACTT TGAGTGCATT 
GGCACTGCTC AGACCGACGA TGAGAATGTC ATTTGTGAAA GTCTCAAGCG ATTTGGTGCT 
ATAATTGGCA ATATTGAAGA TGAGCGCGAA AAAATGCTAA CGCTCGCCGA TAAACATATT 
ATTGAGTCAC TGGAGGATTT TCGAAAAAAG CAAATCGGTG GCGTCAAAGA AAACAAAAAG 
AAATTTGATA AAAAGACGGA GAAATTTTGT CAATCGCAGG AGCGTTTCCT CAATATGTCA 
ACTAAAAAGC CCGAAAACAC AATACAGGAG GCTGACGCCA GTTTGGGGAT GCATGAACGC 
GAGTATATCC AGGAGTCGCT GAGCTATGTG CTGCGCATCC AAGAAGTGCA GGAACGAATC 
AAATTCGAAT TTGTCGAAAT ATTGCTAGCA TTTATCTCTG GCTGGCTGGT TTTCTACCAC 
ACGGCGCACG AACAGGCGGA GGATCATCGT GATTATCTGC AGGATTTGAG GCACAAAGTT 
CAAAAAACTC GGGAAAATTT CGAGGAGGCA CGCGAAAAAG TGACTGAATT GAAAACAAAA 
TATATGGAAA AACGCACAAA GCCCGAGGAA ATTTTTACAA AACGTGGCTA CCTATTTCTA 
ATGGAGAAAA GTTCCATTCT GAAAATCTCC CTTTTAGAGC CATTCAAAGC CACCTGGACA 
AAATACTATT GTACATTCAA GAAGCAAAAG CGCGAGTTCA CTATGCTCCA GTTCAACCAG 
ATGAATCACA ACTTCACGAG ACCCGAGGCG CGTGACGACG AGAAACTGAC GCTTTTCTCC 
TGCCAGCGAC GGGCATCTGA GTTCGAGAAG AGGTTCTGCT TCGATTTGAC CTTCAAGGAG 
AAGCCAGGCG TCGTCTACAC ATTTCAGGCT TTGAGCGAAA AGGATCATCG CTATTGGATT 
AGTGCCATGG ATGGCACGGA GCCGACATAT CTGGCTCCCG GCAAAATCAA AGTCAGCGAG 
GCATACCATC TGGATGAAGC CGGATTCATG TTCATCCGCA GATGCATTCA AGTATTAGAG 
ATTCGAGGCC TGGAGGATGA GGGCATATAC AGAAAGAGCG GTGTTGGAAC CAAAATCAGC 
AAGCTGCTAG CACTGGGCCT CAATCAAAAG GAGAGCGACG ATGTCTTCGT CGACGACAAG 
TACCGCGATT TGATGGAAAG CAACACCATA GCTAGTGCCC TAAAAATGTA TCTTCGAAAT 
CTGAATGAGC CATTGATGAC GTATCAATAT CATAGTGATT TCATTGAAGC CGCAAAACAA 
GAAACCCTCA ATCAGCGCGT GAATGAGGTG CACAAGCTGG TCTACAAGTT GCCGCAGCCC 
AATTTTCAGA TGCTCGACAT GGTTATTTGC CATCTGACTG ACGTTTCGCG CAAGTACGAG 
AAGAACAAAA TGTCCGTATT TAATCTGGGA GTGGTGTTTG GGCCAACATT GTTGCGTCCT 
CGAGAGGAAT CAGTTGCCGC CATATTGGAT ATTAAGTTTA ATAATATCGT CATCAACATT 
CTAATTGATA ACTACGAGAG GATATTCAAG AACAAGCCCA GCGCCGATAT CAAGTTGCCG 
GACGCCACGA AGGCACCCAG CATCATGTAC CCCAGTCGCA GCAGTCCACC GACCAGAATG 
CCGCGAGCCT CTCAAATCGG CAAGGCCGCT TCAACGGGTG CAATGGGTAG CAGCAGTAAT 
ACAGCGGGCA ATCCGATGTT CTTGAATCAG CAGAAAATCT ATCGCGTTGT GAGCAAAACC 
AACTGCACCG AGCCCACAAT GTCATCCAGC CTGCAAAATA TTCCGAATGG CGATAACTAT 
GCCCTTGGCT CGAACGCCAT GAATAGCTCG GGCGGTGCTC AATGCATATC CCTGTCGCCG 
CCGATGCACA TGCTGAACGG CATACTCTCG CCGACCATTG GTAGCATCAA TAATCTGCAC 
ACAATATCGA AAAATGAGAC CAGCACACGA CGATACGTGC CCGACACTGA GATTGCGCCC 
GATTATGGTA TTATAGGTGC TAACAACGGC GGTGTGACGC TGCAATCGCA TCACGCTGTG 
CCGGTTAGTA GCACGAGTAA CTTTCGACAT CCCGAGTACC TAATGACCAC GGCCAATCCA 
GTTACTCAAT CGGGCTCAAG TAGCCATATA TATACGAACA CATCTAGTGC TGGTGCTAAC 
TCAAGCAATC GGATTAGTCT AAGCAATGTA TCCCCGCCAA ATACTGCAAT GCGCAAGGAG 
CGATTCCTGG GCAGCGCCAG TGGTCCGCAG CAGCATCCGC CCGTTCAACG GGGTCTGCAC 
TCGTACGGCC AGACAAAACA CTATTCACCC CTAATGCCAA CGTCAACATC CAGCTCCAAC 
GACAGCGTAT GTGATTCACT ATCTTCCAAC AACGGCCTGG GCAGCTCCAT TGTGGCCAAT 
AGTGGCAACA GTGGGAATGT TGCCCAGCAC TTGAGCGCAC GGAACACTAA TGACTATGCG 
CTGATATCGT CACAGAATTC ATCGCTGTCG CCGCATCTTG GACCCACCTG CGACGAGGTC 
GTCACGGCCA CAACATTGCC CTCGGTTAGT TTAAGCTGTG GTGGCACCGC CAGCGAAAGC 
ACCGAATATC CGCCTAGTAA AATGCATCGT AATCGTGACG TCAACCAAAT AAAACGTGAT 
TTGTCAACGG GCACCGCTCG TGTGCGCACA CTCTATGCTT GCATGGGAGA AAGCGAGGGC 
GAGCTTTCTT TCGAGCCCAA CCAAATAATA ACCAATGTTC GTTACTCCCA CGAACCCGGC 
TGGCTGCAGG GCACACTAAA TGGGAAAACA GGACTCATTC CGGAGAACTA TGTGGAACAT 
TTGAAGCCGC ACCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996441.2
CDS	753..3830
Translation	MGGGKNVRRGLEPLEFEECIVDSPDFRENLNRHEKELDHTSHQIKRIIKEVKDLMSAAKILSTRMKQLAILLNDFNFECIGTAQTDDENVICESLKRFGAIIGNIEDEREKMLTLADKHIIESLEDFRKKQIGGVKENKKKFDKKTEKFCQSQERFLNMSTKKPENTIQEADASLGMHEREYIQESLSYVLRIQEVQERIKFEFVEILLAFISGWLVFYHTAHEQAEDHRDYLQDLRHKVQKTRENFEEAREKVTELKTKYMEKRTKPEEIFTKRGYLFLMEKSSILKISLLEPFKATWTKYYCTFKKQKREFTMLQFNQMNHNFTRPEARDDEKLTLFSCQRRASEFEKRFCFDLTFKEKPGVVYTFQALSEKDHRYWISAMDGTEPTYLAPGKIKVSEAYHLDEAGFMFIRRCIQVLEIRGLEDEGIYRKSGVGTKISKLLALGLNQKESDDVFVDDKYRDLMESNTIASALKMYLRNLNEPLMTYQYHSDFIEAAKQETLNQRVNEVHKLVYKLPQPNFQMLDMVICHLTDVSRKYEKNKMSVFNLGVVFGPTLLRPREESVAAILDIKFNNIVINILIDNYERIFKNKPSADIKLPDATKAPSIMYPSRSSPPTRMPRASQIGKAASTGAMGSSSNTAGNPMFLNQQKIYRVVSKTNCTEPTMSSSLQNIPNGDNYALGSNAMNSSGGAQCISLSPPMHMLNGILSPTIGSINNLHTISKNETSTRRYVPDTEIAPDYGIIGANNGGVTLQSHHAVPVSSTSNFRHPEYLMTTANPVTQSGSSSHIYTNTSSAGANSSNRISLSNVSPPNTAMRKERFLGSASGPQQHPPVQRGLHSYGQTKHYSPLMPTSTSSSNDSVCDSLSSNNGLGSSIVANSGNSGNVAQHLSARNTNDYALISSQNSSLSPHLGPTCDEVVTATTLPSVSLSCGGTASESTEYPPSKMHRNRDVNQIKRDLSTGTARVRTLYACMGESEGELSFEPNQIITNVRYSHEPGWLQGTLNGKTGLIPENYVEHLKPHH

Target ORF information:

RefSeq Version	NM_206718.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster GTPase regulator associated with FAK (Graf), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206718.2

ORF Insert Sequence:

ATGGGCGGCG GCAAAAATGT ACGCCGCGGC TTGGAGCCTC TGGAGTTCGA AGAATGCATC 
GTCGACAGTC CGGACTTTCG CGAAAATTTA AATCGACATG AAAAGGAACT GGACCACACT 
AGTCATCAAA TAAAACGAAT TATCAAAGAG GTCAAAGATC TTATGAGTGC GGCAAAGATC 
CTGTCCACTC GAATGAAACA ATTGGCCATT TTACTTAACG ATTTTAACTT TGAGTGCATT 
GGCACTGCTC AGACCGACGA TGAGAATGTC ATTTGTGAAA GTCTCAAGCG ATTTGGTGCT 
ATAATTGGCA ATATTGAAGA TGAGCGCGAA AAAATGCTAA CGCTCGCCGA TAAACATATT 
ATTGAGTCAC TGGAGGATTT TCGAAAAAAG CAAATCGGTG GCGTCAAAGA AAACAAAAAG 
AAATTTGATA AAAAGACGGA GAAATTTTGT CAATCGCAGG AGCGTTTCCT CAATATGTCA 
ACTAAAAAGC CCGAAAACAC AATACAGGAG GCTGACGCCA GTTTGGGGAT GCATGAACGC 
GAGTATATCC AGGAGTCGCT GAGCTATGTG CTGCGCATCC AAGAAGTGCA GGAACGAATC 
AAATTCGAAT TTGTCGAAAT ATTGCTAGCA TTTATCTCTG GCTGGCTGGT TTTCTACCAC 
ACGGCGCACG AACAGGCGGA GGATCATCGT GATTATCTGC AGGATTTGAG GCACAAAGTT 
CAAAAAACTC GGGAAAATTT CGAGGAGGCA CGCGAAAAAG TGACTGAATT GAAAACAAAA 
TATATGGAAA AACGCACAAA GCCCGAGGAA ATTTTTACAA AACGTGGCTA CCTATTTCTA 
ATGGAGAAAA GTTCCATTCT GAAAATCTCC CTTTTAGAGC CATTCAAAGC CACCTGGACA 
AAATACTATT GTACATTCAA GAAGCAAAAG CGCGAGTTCA CTATGCTCCA GTTCAACCAG 
ATGAATCACA ACTTCACGAG ACCCGAGGCG CGTGACGACG AGAAACTGAC GCTTTTCTCC 
TGCCAGCGAC GGGCATCTGA GTTCGAGAAG AGGTTCTGCT TCGATTTGAC CTTCAAGGAG 
AAGCCAGGCG TCGTCTACAC ATTTCAGGCT TTGAGCGAAA AGGATCATCG CTATTGGATT 
AGTGCCATGG ATGGCACGGA GCCGACATAT CTGGCTCCCG GCAAAATCAA AGTCAGCGAG 
GCATACCATC TGGATGAAGC CGGATTCATG TTCATCCGCA GATGCATTCA AGTATTAGAG 
ATTCGAGGCC TGGAGGATGA GGGCATATAC AGAAAGAGCG GTGTTGGAAC CAAAATCAGC 
AAGCTGCTAG CACTGGGCCT CAATCAAAAG GAGAGCGACG ATGTCTTCGT CGACGACAAG 
TACCGCGATT TGATGGAAAG CAACACCATA GCTAGTGCCC TAAAAATGTA TCTTCGAAAT 
CTGAATGAGC CATTGATGAC GTATCAATAT CATAGTGATT TCATTGAAGC CGCAAAACAA 
GAAACCCTCA ATCAGCGCGT GAATGAGGTG CACAAGCTGG TCTACAAGTT GCCGCAGCCC 
AATTTTCAGA TGCTCGACAT GGTTATTTGC CATCTGACTG ACGTTTCGCG CAAGTACGAG 
AAGAACAAAA TGTCCGTATT TAATCTGGGA GTGGTGTTTG GGCCAACATT GTTGCGTCCT 
CGAGAGGAAT CAGTTGCCGC CATATTGGAT ATTAAGTTTA ATAATATCGT CATCAACATT 
CTAATTGATA ACTACGAGAG GATATTCAAG AACAAGCCCA GCGCCGATAT CAAGTTGCCG 
GACGCCACGA AGGCACCCAG CATCATGTAC CCCAGTCGCA GCAGTCCACC GACCAGAATG 
CCGCGAGCCT CTCAAATCGG CAAGGCCGCT TCAACGGGTG CAATGGGTAG CAGCAGTAAT 
ACAGCGGGCA ATCCGATGTT CTTGAATCAG CAGAAAATCT ATCGCGTTGT GAGCAAAACC 
AACTGCACCG AGCCCACAAT GTCATCCAGC CTGCAAAATA TTCCGAATGG CGATAACTAT 
GCCCTTGGCT CGAACGCCAT GAATAGCTCG GGCGGTGCTC AATGCATATC CCTGTCGCCG 
CCGATGCACA TGCTGAACGG CATACTCTCG CCGACCATTG GTAGCATCAA TAATCTGCAC 
ACAATATCGA AAAATGAGAC CAGCACACGA CGATACGTGC CCGACACTGA GATTGCGCCC 
GATTATGGTA TTATAGGTGC TAACAACGGC GGTGTGACGC TGCAATCGCA TCACGCTGTG 
CCGGTTAGTA GCACGAGTAA CTTTCGACAT CCCGAGTACC TAATGACCAC GGCCAATCCA 
GTTACTCAAT CGGGCTCAAG TAGCCATATA TATACGAACA CATCTAGTGC TGGTGCTAAC 
TCAAGCAATC GGATTAGTCT AAGCAATGTA TCCCCGCCAA ATACTGCAAT GCGCAAGGAG 
CGATTCCTGG GCAGCGCCAG TGGTCCGCAG CAGCATCCGC CCGTTCAACG GGGTCTGCAC 
TCGTACGGCC AGACAAAACA CTATTCACCC CTAATGCCAA CGTCAACATC CAGCTCCAAC 
GACAGCGTAT GTGATTCACT ATCTTCCAAC AACGGCCTGG GCAGCTCCAT TGTGGCCAAT 
AGTGGCAACA GTGGGAATGT TGCCCAGCAC TTGAGCGCAC GGAACACTAA TGACTATGCG 
CTGATATCGT CACAGAATTC ATCGCTGTCG CCGCATCTTG GACCCACCTG CGACGAGGTC 
GTCACGGCCA CAACATTGCC CTCGGTTAGT TTAAGCTGTG GTGGCACCGC CAGCGAAAGC 
ACCGAATATC CGCCTAGTAA AATGCATCGT AATCGTGACG TCAACCAAAT AAAACGTGAT 
TTGTCAACGG GCACCGCTCG TGTGCGCACA CTCTATGCTT GCATGGGAGA AAGCGAGGGC 
GAGCTTTCTT TCGAGCCCAA CCAAATAATA ACCAATGTTC GTTACTCCCA CGAACCCGGC 
TGGCTGCAGG GCACACTAAA TGGGAAAACA GGACTCATTC CGGAGAACTA TGTGGAACAT 
TTGAAGCCGC ACCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Graf ( NM_206721.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996444.2 Drosophila melanogaster GTPase regulator associated with FAK (Graf), transcript variant I, mRNA.

Price & Availability↑

CloneID	OFa21492
Clone ID Related Accession (Same CDS sequence)	NM_206718.2 , NM_206721.2 , NM_001298367.1
Accession Version	NM_206721.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3078bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	GTPase regulator associated with FAK, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_206721.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCGGCG GCAAAAATGT ACGCCGCGGC TTGGAGCCTC TGGAGTTCGA AGAATGCATC 
GTCGACAGTC CGGACTTTCG CGAAAATTTA AATCGACATG AAAAGGAACT GGACCACACT 
AGTCATCAAA TAAAACGAAT TATCAAAGAG GTCAAAGATC TTATGAGTGC GGCAAAGATC 
CTGTCCACTC GAATGAAACA ATTGGCCATT TTACTTAACG ATTTTAACTT TGAGTGCATT 
GGCACTGCTC AGACCGACGA TGAGAATGTC ATTTGTGAAA GTCTCAAGCG ATTTGGTGCT 
ATAATTGGCA ATATTGAAGA TGAGCGCGAA AAAATGCTAA CGCTCGCCGA TAAACATATT 
ATTGAGTCAC TGGAGGATTT TCGAAAAAAG CAAATCGGTG GCGTCAAAGA AAACAAAAAG 
AAATTTGATA AAAAGACGGA GAAATTTTGT CAATCGCAGG AGCGTTTCCT CAATATGTCA 
ACTAAAAAGC CCGAAAACAC AATACAGGAG GCTGACGCCA GTTTGGGGAT GCATGAACGC 
GAGTATATCC AGGAGTCGCT GAGCTATGTG CTGCGCATCC AAGAAGTGCA GGAACGAATC 
AAATTCGAAT TTGTCGAAAT ATTGCTAGCA TTTATCTCTG GCTGGCTGGT TTTCTACCAC 
ACGGCGCACG AACAGGCGGA GGATCATCGT GATTATCTGC AGGATTTGAG GCACAAAGTT 
CAAAAAACTC GGGAAAATTT CGAGGAGGCA CGCGAAAAAG TGACTGAATT GAAAACAAAA 
TATATGGAAA AACGCACAAA GCCCGAGGAA ATTTTTACAA AACGTGGCTA CCTATTTCTA 
ATGGAGAAAA GTTCCATTCT GAAAATCTCC CTTTTAGAGC CATTCAAAGC CACCTGGACA 
AAATACTATT GTACATTCAA GAAGCAAAAG CGCGAGTTCA CTATGCTCCA GTTCAACCAG 
ATGAATCACA ACTTCACGAG ACCCGAGGCG CGTGACGACG AGAAACTGAC GCTTTTCTCC 
TGCCAGCGAC GGGCATCTGA GTTCGAGAAG AGGTTCTGCT TCGATTTGAC CTTCAAGGAG 
AAGCCAGGCG TCGTCTACAC ATTTCAGGCT TTGAGCGAAA AGGATCATCG CTATTGGATT 
AGTGCCATGG ATGGCACGGA GCCGACATAT CTGGCTCCCG GCAAAATCAA AGTCAGCGAG 
GCATACCATC TGGATGAAGC CGGATTCATG TTCATCCGCA GATGCATTCA AGTATTAGAG 
ATTCGAGGCC TGGAGGATGA GGGCATATAC AGAAAGAGCG GTGTTGGAAC CAAAATCAGC 
AAGCTGCTAG CACTGGGCCT CAATCAAAAG GAGAGCGACG ATGTCTTCGT CGACGACAAG 
TACCGCGATT TGATGGAAAG CAACACCATA GCTAGTGCCC TAAAAATGTA TCTTCGAAAT 
CTGAATGAGC CATTGATGAC GTATCAATAT CATAGTGATT TCATTGAAGC CGCAAAACAA 
GAAACCCTCA ATCAGCGCGT GAATGAGGTG CACAAGCTGG TCTACAAGTT GCCGCAGCCC 
AATTTTCAGA TGCTCGACAT GGTTATTTGC CATCTGACTG ACGTTTCGCG CAAGTACGAG 
AAGAACAAAA TGTCCGTATT TAATCTGGGA GTGGTGTTTG GGCCAACATT GTTGCGTCCT 
CGAGAGGAAT CAGTTGCCGC CATATTGGAT ATTAAGTTTA ATAATATCGT CATCAACATT 
CTAATTGATA ACTACGAGAG GATATTCAAG AACAAGCCCA GCGCCGATAT CAAGTTGCCG 
GACGCCACGA AGGCACCCAG CATCATGTAC CCCAGTCGCA GCAGTCCACC GACCAGAATG 
CCGCGAGCCT CTCAAATCGG CAAGGCCGCT TCAACGGGTG CAATGGGTAG CAGCAGTAAT 
ACAGCGGGCA ATCCGATGTT CTTGAATCAG CAGAAAATCT ATCGCGTTGT GAGCAAAACC 
AACTGCACCG AGCCCACAAT GTCATCCAGC CTGCAAAATA TTCCGAATGG CGATAACTAT 
GCCCTTGGCT CGAACGCCAT GAATAGCTCG GGCGGTGCTC AATGCATATC CCTGTCGCCG 
CCGATGCACA TGCTGAACGG CATACTCTCG CCGACCATTG GTAGCATCAA TAATCTGCAC 
ACAATATCGA AAAATGAGAC CAGCACACGA CGATACGTGC CCGACACTGA GATTGCGCCC 
GATTATGGTA TTATAGGTGC TAACAACGGC GGTGTGACGC TGCAATCGCA TCACGCTGTG 
CCGGTTAGTA GCACGAGTAA CTTTCGACAT CCCGAGTACC TAATGACCAC GGCCAATCCA 
GTTACTCAAT CGGGCTCAAG TAGCCATATA TATACGAACA CATCTAGTGC TGGTGCTAAC 
TCAAGCAATC GGATTAGTCT AAGCAATGTA TCCCCGCCAA ATACTGCAAT GCGCAAGGAG 
CGATTCCTGG GCAGCGCCAG TGGTCCGCAG CAGCATCCGC CCGTTCAACG GGGTCTGCAC 
TCGTACGGCC AGACAAAACA CTATTCACCC CTAATGCCAA CGTCAACATC CAGCTCCAAC 
GACAGCGTAT GTGATTCACT ATCTTCCAAC AACGGCCTGG GCAGCTCCAT TGTGGCCAAT 
AGTGGCAACA GTGGGAATGT TGCCCAGCAC TTGAGCGCAC GGAACACTAA TGACTATGCG 
CTGATATCGT CACAGAATTC ATCGCTGTCG CCGCATCTTG GACCCACCTG CGACGAGGTC 
GTCACGGCCA CAACATTGCC CTCGGTTAGT TTAAGCTGTG GTGGCACCGC CAGCGAAAGC 
ACCGAATATC CGCCTAGTAA AATGCATCGT AATCGTGACG TCAACCAAAT AAAACGTGAT 
TTGTCAACGG GCACCGCTCG TGTGCGCACA CTCTATGCTT GCATGGGAGA AAGCGAGGGC 
GAGCTTTCTT TCGAGCCCAA CCAAATAATA ACCAATGTTC GTTACTCCCA CGAACCCGGC 
TGGCTGCAGG GCACACTAAA TGGGAAAACA GGACTCATTC CGGAGAACTA TGTGGAACAT 
TTGAAGCCGC ACCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996444.2
CDS	695..3772
Translation	MGGGKNVRRGLEPLEFEECIVDSPDFRENLNRHEKELDHTSHQIKRIIKEVKDLMSAAKILSTRMKQLAILLNDFNFECIGTAQTDDENVICESLKRFGAIIGNIEDEREKMLTLADKHIIESLEDFRKKQIGGVKENKKKFDKKTEKFCQSQERFLNMSTKKPENTIQEADASLGMHEREYIQESLSYVLRIQEVQERIKFEFVEILLAFISGWLVFYHTAHEQAEDHRDYLQDLRHKVQKTRENFEEAREKVTELKTKYMEKRTKPEEIFTKRGYLFLMEKSSILKISLLEPFKATWTKYYCTFKKQKREFTMLQFNQMNHNFTRPEARDDEKLTLFSCQRRASEFEKRFCFDLTFKEKPGVVYTFQALSEKDHRYWISAMDGTEPTYLAPGKIKVSEAYHLDEAGFMFIRRCIQVLEIRGLEDEGIYRKSGVGTKISKLLALGLNQKESDDVFVDDKYRDLMESNTIASALKMYLRNLNEPLMTYQYHSDFIEAAKQETLNQRVNEVHKLVYKLPQPNFQMLDMVICHLTDVSRKYEKNKMSVFNLGVVFGPTLLRPREESVAAILDIKFNNIVINILIDNYERIFKNKPSADIKLPDATKAPSIMYPSRSSPPTRMPRASQIGKAASTGAMGSSSNTAGNPMFLNQQKIYRVVSKTNCTEPTMSSSLQNIPNGDNYALGSNAMNSSGGAQCISLSPPMHMLNGILSPTIGSINNLHTISKNETSTRRYVPDTEIAPDYGIIGANNGGVTLQSHHAVPVSSTSNFRHPEYLMTTANPVTQSGSSSHIYTNTSSAGANSSNRISLSNVSPPNTAMRKERFLGSASGPQQHPPVQRGLHSYGQTKHYSPLMPTSTSSSNDSVCDSLSSNNGLGSSIVANSGNSGNVAQHLSARNTNDYALISSQNSSLSPHLGPTCDEVVTATTLPSVSLSCGGTASESTEYPPSKMHRNRDVNQIKRDLSTGTARVRTLYACMGESEGELSFEPNQIITNVRYSHEPGWLQGTLNGKTGLIPENYVEHLKPHH

Target ORF information:

RefSeq Version	NM_206721.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster GTPase regulator associated with FAK (Graf), transcript variant I, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206721.2

ORF Insert Sequence:

ATGGGCGGCG GCAAAAATGT ACGCCGCGGC TTGGAGCCTC TGGAGTTCGA AGAATGCATC 
GTCGACAGTC CGGACTTTCG CGAAAATTTA AATCGACATG AAAAGGAACT GGACCACACT 
AGTCATCAAA TAAAACGAAT TATCAAAGAG GTCAAAGATC TTATGAGTGC GGCAAAGATC 
CTGTCCACTC GAATGAAACA ATTGGCCATT TTACTTAACG ATTTTAACTT TGAGTGCATT 
GGCACTGCTC AGACCGACGA TGAGAATGTC ATTTGTGAAA GTCTCAAGCG ATTTGGTGCT 
ATAATTGGCA ATATTGAAGA TGAGCGCGAA AAAATGCTAA CGCTCGCCGA TAAACATATT 
ATTGAGTCAC TGGAGGATTT TCGAAAAAAG CAAATCGGTG GCGTCAAAGA AAACAAAAAG 
AAATTTGATA AAAAGACGGA GAAATTTTGT CAATCGCAGG AGCGTTTCCT CAATATGTCA 
ACTAAAAAGC CCGAAAACAC AATACAGGAG GCTGACGCCA GTTTGGGGAT GCATGAACGC 
GAGTATATCC AGGAGTCGCT GAGCTATGTG CTGCGCATCC AAGAAGTGCA GGAACGAATC 
AAATTCGAAT TTGTCGAAAT ATTGCTAGCA TTTATCTCTG GCTGGCTGGT TTTCTACCAC 
ACGGCGCACG AACAGGCGGA GGATCATCGT GATTATCTGC AGGATTTGAG GCACAAAGTT 
CAAAAAACTC GGGAAAATTT CGAGGAGGCA CGCGAAAAAG TGACTGAATT GAAAACAAAA 
TATATGGAAA AACGCACAAA GCCCGAGGAA ATTTTTACAA AACGTGGCTA CCTATTTCTA 
ATGGAGAAAA GTTCCATTCT GAAAATCTCC CTTTTAGAGC CATTCAAAGC CACCTGGACA 
AAATACTATT GTACATTCAA GAAGCAAAAG CGCGAGTTCA CTATGCTCCA GTTCAACCAG 
ATGAATCACA ACTTCACGAG ACCCGAGGCG CGTGACGACG AGAAACTGAC GCTTTTCTCC 
TGCCAGCGAC GGGCATCTGA GTTCGAGAAG AGGTTCTGCT TCGATTTGAC CTTCAAGGAG 
AAGCCAGGCG TCGTCTACAC ATTTCAGGCT TTGAGCGAAA AGGATCATCG CTATTGGATT 
AGTGCCATGG ATGGCACGGA GCCGACATAT CTGGCTCCCG GCAAAATCAA AGTCAGCGAG 
GCATACCATC TGGATGAAGC CGGATTCATG TTCATCCGCA GATGCATTCA AGTATTAGAG 
ATTCGAGGCC TGGAGGATGA GGGCATATAC AGAAAGAGCG GTGTTGGAAC CAAAATCAGC 
AAGCTGCTAG CACTGGGCCT CAATCAAAAG GAGAGCGACG ATGTCTTCGT CGACGACAAG 
TACCGCGATT TGATGGAAAG CAACACCATA GCTAGTGCCC TAAAAATGTA TCTTCGAAAT 
CTGAATGAGC CATTGATGAC GTATCAATAT CATAGTGATT TCATTGAAGC CGCAAAACAA 
GAAACCCTCA ATCAGCGCGT GAATGAGGTG CACAAGCTGG TCTACAAGTT GCCGCAGCCC 
AATTTTCAGA TGCTCGACAT GGTTATTTGC CATCTGACTG ACGTTTCGCG CAAGTACGAG 
AAGAACAAAA TGTCCGTATT TAATCTGGGA GTGGTGTTTG GGCCAACATT GTTGCGTCCT 
CGAGAGGAAT CAGTTGCCGC CATATTGGAT ATTAAGTTTA ATAATATCGT CATCAACATT 
CTAATTGATA ACTACGAGAG GATATTCAAG AACAAGCCCA GCGCCGATAT CAAGTTGCCG 
GACGCCACGA AGGCACCCAG CATCATGTAC CCCAGTCGCA GCAGTCCACC GACCAGAATG 
CCGCGAGCCT CTCAAATCGG CAAGGCCGCT TCAACGGGTG CAATGGGTAG CAGCAGTAAT 
ACAGCGGGCA ATCCGATGTT CTTGAATCAG CAGAAAATCT ATCGCGTTGT GAGCAAAACC 
AACTGCACCG AGCCCACAAT GTCATCCAGC CTGCAAAATA TTCCGAATGG CGATAACTAT 
GCCCTTGGCT CGAACGCCAT GAATAGCTCG GGCGGTGCTC AATGCATATC CCTGTCGCCG 
CCGATGCACA TGCTGAACGG CATACTCTCG CCGACCATTG GTAGCATCAA TAATCTGCAC 
ACAATATCGA AAAATGAGAC CAGCACACGA CGATACGTGC CCGACACTGA GATTGCGCCC 
GATTATGGTA TTATAGGTGC TAACAACGGC GGTGTGACGC TGCAATCGCA TCACGCTGTG 
CCGGTTAGTA GCACGAGTAA CTTTCGACAT CCCGAGTACC TAATGACCAC GGCCAATCCA 
GTTACTCAAT CGGGCTCAAG TAGCCATATA TATACGAACA CATCTAGTGC TGGTGCTAAC 
TCAAGCAATC GGATTAGTCT AAGCAATGTA TCCCCGCCAA ATACTGCAAT GCGCAAGGAG 
CGATTCCTGG GCAGCGCCAG TGGTCCGCAG CAGCATCCGC CCGTTCAACG GGGTCTGCAC 
TCGTACGGCC AGACAAAACA CTATTCACCC CTAATGCCAA CGTCAACATC CAGCTCCAAC 
GACAGCGTAT GTGATTCACT ATCTTCCAAC AACGGCCTGG GCAGCTCCAT TGTGGCCAAT 
AGTGGCAACA GTGGGAATGT TGCCCAGCAC TTGAGCGCAC GGAACACTAA TGACTATGCG 
CTGATATCGT CACAGAATTC ATCGCTGTCG CCGCATCTTG GACCCACCTG CGACGAGGTC 
GTCACGGCCA CAACATTGCC CTCGGTTAGT TTAAGCTGTG GTGGCACCGC CAGCGAAAGC 
ACCGAATATC CGCCTAGTAA AATGCATCGT AATCGTGACG TCAACCAAAT AAAACGTGAT 
TTGTCAACGG GCACCGCTCG TGTGCGCACA CTCTATGCTT GCATGGGAGA AAGCGAGGGC 
GAGCTTTCTT TCGAGCCCAA CCAAATAATA ACCAATGTTC GTTACTCCCA CGAACCCGGC 
TGGCTGCAGG GCACACTAAA TGGGAAAACA GGACTCATTC CGGAGAACTA TGTGGAACAT 
TTGAAGCCGC ACCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Graf ( NM_001298367.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285296.1 Drosophila melanogaster GTPase regulator associated with FAK (Graf), transcript variant H, mRNA.

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CloneID	OFa21492
Clone ID Related Accession (Same CDS sequence)	NM_206718.2 , NM_206721.2 , NM_001298367.1
Accession Version	NM_001298367.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3078bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	GTPase regulator associated with FAK, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCGGCG GCAAAAATGT ACGCCGCGGC TTGGAGCCTC TGGAGTTCGA AGAATGCATC 
GTCGACAGTC CGGACTTTCG CGAAAATTTA AATCGACATG AAAAGGAACT GGACCACACT 
AGTCATCAAA TAAAACGAAT TATCAAAGAG GTCAAAGATC TTATGAGTGC GGCAAAGATC 
CTGTCCACTC GAATGAAACA ATTGGCCATT TTACTTAACG ATTTTAACTT TGAGTGCATT 
GGCACTGCTC AGACCGACGA TGAGAATGTC ATTTGTGAAA GTCTCAAGCG ATTTGGTGCT 
ATAATTGGCA ATATTGAAGA TGAGCGCGAA AAAATGCTAA CGCTCGCCGA TAAACATATT 
ATTGAGTCAC TGGAGGATTT TCGAAAAAAG CAAATCGGTG GCGTCAAAGA AAACAAAAAG 
AAATTTGATA AAAAGACGGA GAAATTTTGT CAATCGCAGG AGCGTTTCCT CAATATGTCA 
ACTAAAAAGC CCGAAAACAC AATACAGGAG GCTGACGCCA GTTTGGGGAT GCATGAACGC 
GAGTATATCC AGGAGTCGCT GAGCTATGTG CTGCGCATCC AAGAAGTGCA GGAACGAATC 
AAATTCGAAT TTGTCGAAAT ATTGCTAGCA TTTATCTCTG GCTGGCTGGT TTTCTACCAC 
ACGGCGCACG AACAGGCGGA GGATCATCGT GATTATCTGC AGGATTTGAG GCACAAAGTT 
CAAAAAACTC GGGAAAATTT CGAGGAGGCA CGCGAAAAAG TGACTGAATT GAAAACAAAA 
TATATGGAAA AACGCACAAA GCCCGAGGAA ATTTTTACAA AACGTGGCTA CCTATTTCTA 
ATGGAGAAAA GTTCCATTCT GAAAATCTCC CTTTTAGAGC CATTCAAAGC CACCTGGACA 
AAATACTATT GTACATTCAA GAAGCAAAAG CGCGAGTTCA CTATGCTCCA GTTCAACCAG 
ATGAATCACA ACTTCACGAG ACCCGAGGCG CGTGACGACG AGAAACTGAC GCTTTTCTCC 
TGCCAGCGAC GGGCATCTGA GTTCGAGAAG AGGTTCTGCT TCGATTTGAC CTTCAAGGAG 
AAGCCAGGCG TCGTCTACAC ATTTCAGGCT TTGAGCGAAA AGGATCATCG CTATTGGATT 
AGTGCCATGG ATGGCACGGA GCCGACATAT CTGGCTCCCG GCAAAATCAA AGTCAGCGAG 
GCATACCATC TGGATGAAGC CGGATTCATG TTCATCCGCA GATGCATTCA AGTATTAGAG 
ATTCGAGGCC TGGAGGATGA GGGCATATAC AGAAAGAGCG GTGTTGGAAC CAAAATCAGC 
AAGCTGCTAG CACTGGGCCT CAATCAAAAG GAGAGCGACG ATGTCTTCGT CGACGACAAG 
TACCGCGATT TGATGGAAAG CAACACCATA GCTAGTGCCC TAAAAATGTA TCTTCGAAAT 
CTGAATGAGC CATTGATGAC GTATCAATAT CATAGTGATT TCATTGAAGC CGCAAAACAA 
GAAACCCTCA ATCAGCGCGT GAATGAGGTG CACAAGCTGG TCTACAAGTT GCCGCAGCCC 
AATTTTCAGA TGCTCGACAT GGTTATTTGC CATCTGACTG ACGTTTCGCG CAAGTACGAG 
AAGAACAAAA TGTCCGTATT TAATCTGGGA GTGGTGTTTG GGCCAACATT GTTGCGTCCT 
CGAGAGGAAT CAGTTGCCGC CATATTGGAT ATTAAGTTTA ATAATATCGT CATCAACATT 
CTAATTGATA ACTACGAGAG GATATTCAAG AACAAGCCCA GCGCCGATAT CAAGTTGCCG 
GACGCCACGA AGGCACCCAG CATCATGTAC CCCAGTCGCA GCAGTCCACC GACCAGAATG 
CCGCGAGCCT CTCAAATCGG CAAGGCCGCT TCAACGGGTG CAATGGGTAG CAGCAGTAAT 
ACAGCGGGCA ATCCGATGTT CTTGAATCAG CAGAAAATCT ATCGCGTTGT GAGCAAAACC 
AACTGCACCG AGCCCACAAT GTCATCCAGC CTGCAAAATA TTCCGAATGG CGATAACTAT 
GCCCTTGGCT CGAACGCCAT GAATAGCTCG GGCGGTGCTC AATGCATATC CCTGTCGCCG 
CCGATGCACA TGCTGAACGG CATACTCTCG CCGACCATTG GTAGCATCAA TAATCTGCAC 
ACAATATCGA AAAATGAGAC CAGCACACGA CGATACGTGC CCGACACTGA GATTGCGCCC 
GATTATGGTA TTATAGGTGC TAACAACGGC GGTGTGACGC TGCAATCGCA TCACGCTGTG 
CCGGTTAGTA GCACGAGTAA CTTTCGACAT CCCGAGTACC TAATGACCAC GGCCAATCCA 
GTTACTCAAT CGGGCTCAAG TAGCCATATA TATACGAACA CATCTAGTGC TGGTGCTAAC 
TCAAGCAATC GGATTAGTCT AAGCAATGTA TCCCCGCCAA ATACTGCAAT GCGCAAGGAG 
CGATTCCTGG GCAGCGCCAG TGGTCCGCAG CAGCATCCGC CCGTTCAACG GGGTCTGCAC 
TCGTACGGCC AGACAAAACA CTATTCACCC CTAATGCCAA CGTCAACATC CAGCTCCAAC 
GACAGCGTAT GTGATTCACT ATCTTCCAAC AACGGCCTGG GCAGCTCCAT TGTGGCCAAT 
AGTGGCAACA GTGGGAATGT TGCCCAGCAC TTGAGCGCAC GGAACACTAA TGACTATGCG 
CTGATATCGT CACAGAATTC ATCGCTGTCG CCGCATCTTG GACCCACCTG CGACGAGGTC 
GTCACGGCCA CAACATTGCC CTCGGTTAGT TTAAGCTGTG GTGGCACCGC CAGCGAAAGC 
ACCGAATATC CGCCTAGTAA AATGCATCGT AATCGTGACG TCAACCAAAT AAAACGTGAT 
TTGTCAACGG GCACCGCTCG TGTGCGCACA CTCTATGCTT GCATGGGAGA AAGCGAGGGC 
GAGCTTTCTT TCGAGCCCAA CCAAATAATA ACCAATGTTC GTTACTCCCA CGAACCCGGC 
TGGCTGCAGG GCACACTAAA TGGGAAAACA GGACTCATTC CGGAGAACTA TGTGGAACAT 
TTGAAGCCGC ACCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285296.1
CDS	468..3545
Translation	MGGGKNVRRGLEPLEFEECIVDSPDFRENLNRHEKELDHTSHQIKRIIKEVKDLMSAAKILSTRMKQLAILLNDFNFECIGTAQTDDENVICESLKRFGAIIGNIEDEREKMLTLADKHIIESLEDFRKKQIGGVKENKKKFDKKTEKFCQSQERFLNMSTKKPENTIQEADASLGMHEREYIQESLSYVLRIQEVQERIKFEFVEILLAFISGWLVFYHTAHEQAEDHRDYLQDLRHKVQKTRENFEEAREKVTELKTKYMEKRTKPEEIFTKRGYLFLMEKSSILKISLLEPFKATWTKYYCTFKKQKREFTMLQFNQMNHNFTRPEARDDEKLTLFSCQRRASEFEKRFCFDLTFKEKPGVVYTFQALSEKDHRYWISAMDGTEPTYLAPGKIKVSEAYHLDEAGFMFIRRCIQVLEIRGLEDEGIYRKSGVGTKISKLLALGLNQKESDDVFVDDKYRDLMESNTIASALKMYLRNLNEPLMTYQYHSDFIEAAKQETLNQRVNEVHKLVYKLPQPNFQMLDMVICHLTDVSRKYEKNKMSVFNLGVVFGPTLLRPREESVAAILDIKFNNIVINILIDNYERIFKNKPSADIKLPDATKAPSIMYPSRSSPPTRMPRASQIGKAASTGAMGSSSNTAGNPMFLNQQKIYRVVSKTNCTEPTMSSSLQNIPNGDNYALGSNAMNSSGGAQCISLSPPMHMLNGILSPTIGSINNLHTISKNETSTRRYVPDTEIAPDYGIIGANNGGVTLQSHHAVPVSSTSNFRHPEYLMTTANPVTQSGSSSHIYTNTSSAGANSSNRISLSNVSPPNTAMRKERFLGSASGPQQHPPVQRGLHSYGQTKHYSPLMPTSTSSSNDSVCDSLSSNNGLGSSIVANSGNSGNVAQHLSARNTNDYALISSQNSSLSPHLGPTCDEVVTATTLPSVSLSCGGTASESTEYPPSKMHRNRDVNQIKRDLSTGTARVRTLYACMGESEGELSFEPNQIITNVRYSHEPGWLQGTLNGKTGLIPENYVEHLKPHH

Target ORF information:

RefSeq Version	NM_001298367.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster GTPase regulator associated with FAK (Graf), transcript variant H, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298367.1

ORF Insert Sequence:

ATGGGCGGCG GCAAAAATGT ACGCCGCGGC TTGGAGCCTC TGGAGTTCGA AGAATGCATC 
GTCGACAGTC CGGACTTTCG CGAAAATTTA AATCGACATG AAAAGGAACT GGACCACACT 
AGTCATCAAA TAAAACGAAT TATCAAAGAG GTCAAAGATC TTATGAGTGC GGCAAAGATC 
CTGTCCACTC GAATGAAACA ATTGGCCATT TTACTTAACG ATTTTAACTT TGAGTGCATT 
GGCACTGCTC AGACCGACGA TGAGAATGTC ATTTGTGAAA GTCTCAAGCG ATTTGGTGCT 
ATAATTGGCA ATATTGAAGA TGAGCGCGAA AAAATGCTAA CGCTCGCCGA TAAACATATT 
ATTGAGTCAC TGGAGGATTT TCGAAAAAAG CAAATCGGTG GCGTCAAAGA AAACAAAAAG 
AAATTTGATA AAAAGACGGA GAAATTTTGT CAATCGCAGG AGCGTTTCCT CAATATGTCA 
ACTAAAAAGC CCGAAAACAC AATACAGGAG GCTGACGCCA GTTTGGGGAT GCATGAACGC 
GAGTATATCC AGGAGTCGCT GAGCTATGTG CTGCGCATCC AAGAAGTGCA GGAACGAATC 
AAATTCGAAT TTGTCGAAAT ATTGCTAGCA TTTATCTCTG GCTGGCTGGT TTTCTACCAC 
ACGGCGCACG AACAGGCGGA GGATCATCGT GATTATCTGC AGGATTTGAG GCACAAAGTT 
CAAAAAACTC GGGAAAATTT CGAGGAGGCA CGCGAAAAAG TGACTGAATT GAAAACAAAA 
TATATGGAAA AACGCACAAA GCCCGAGGAA ATTTTTACAA AACGTGGCTA CCTATTTCTA 
ATGGAGAAAA GTTCCATTCT GAAAATCTCC CTTTTAGAGC CATTCAAAGC CACCTGGACA 
AAATACTATT GTACATTCAA GAAGCAAAAG CGCGAGTTCA CTATGCTCCA GTTCAACCAG 
ATGAATCACA ACTTCACGAG ACCCGAGGCG CGTGACGACG AGAAACTGAC GCTTTTCTCC 
TGCCAGCGAC GGGCATCTGA GTTCGAGAAG AGGTTCTGCT TCGATTTGAC CTTCAAGGAG 
AAGCCAGGCG TCGTCTACAC ATTTCAGGCT TTGAGCGAAA AGGATCATCG CTATTGGATT 
AGTGCCATGG ATGGCACGGA GCCGACATAT CTGGCTCCCG GCAAAATCAA AGTCAGCGAG 
GCATACCATC TGGATGAAGC CGGATTCATG TTCATCCGCA GATGCATTCA AGTATTAGAG 
ATTCGAGGCC TGGAGGATGA GGGCATATAC AGAAAGAGCG GTGTTGGAAC CAAAATCAGC 
AAGCTGCTAG CACTGGGCCT CAATCAAAAG GAGAGCGACG ATGTCTTCGT CGACGACAAG 
TACCGCGATT TGATGGAAAG CAACACCATA GCTAGTGCCC TAAAAATGTA TCTTCGAAAT 
CTGAATGAGC CATTGATGAC GTATCAATAT CATAGTGATT TCATTGAAGC CGCAAAACAA 
GAAACCCTCA ATCAGCGCGT GAATGAGGTG CACAAGCTGG TCTACAAGTT GCCGCAGCCC 
AATTTTCAGA TGCTCGACAT GGTTATTTGC CATCTGACTG ACGTTTCGCG CAAGTACGAG 
AAGAACAAAA TGTCCGTATT TAATCTGGGA GTGGTGTTTG GGCCAACATT GTTGCGTCCT 
CGAGAGGAAT CAGTTGCCGC CATATTGGAT ATTAAGTTTA ATAATATCGT CATCAACATT 
CTAATTGATA ACTACGAGAG GATATTCAAG AACAAGCCCA GCGCCGATAT CAAGTTGCCG 
GACGCCACGA AGGCACCCAG CATCATGTAC CCCAGTCGCA GCAGTCCACC GACCAGAATG 
CCGCGAGCCT CTCAAATCGG CAAGGCCGCT TCAACGGGTG CAATGGGTAG CAGCAGTAAT 
ACAGCGGGCA ATCCGATGTT CTTGAATCAG CAGAAAATCT ATCGCGTTGT GAGCAAAACC 
AACTGCACCG AGCCCACAAT GTCATCCAGC CTGCAAAATA TTCCGAATGG CGATAACTAT 
GCCCTTGGCT CGAACGCCAT GAATAGCTCG GGCGGTGCTC AATGCATATC CCTGTCGCCG 
CCGATGCACA TGCTGAACGG CATACTCTCG CCGACCATTG GTAGCATCAA TAATCTGCAC 
ACAATATCGA AAAATGAGAC CAGCACACGA CGATACGTGC CCGACACTGA GATTGCGCCC 
GATTATGGTA TTATAGGTGC TAACAACGGC GGTGTGACGC TGCAATCGCA TCACGCTGTG 
CCGGTTAGTA GCACGAGTAA CTTTCGACAT CCCGAGTACC TAATGACCAC GGCCAATCCA 
GTTACTCAAT CGGGCTCAAG TAGCCATATA TATACGAACA CATCTAGTGC TGGTGCTAAC 
TCAAGCAATC GGATTAGTCT AAGCAATGTA TCCCCGCCAA ATACTGCAAT GCGCAAGGAG 
CGATTCCTGG GCAGCGCCAG TGGTCCGCAG CAGCATCCGC CCGTTCAACG GGGTCTGCAC 
TCGTACGGCC AGACAAAACA CTATTCACCC CTAATGCCAA CGTCAACATC CAGCTCCAAC 
GACAGCGTAT GTGATTCACT ATCTTCCAAC AACGGCCTGG GCAGCTCCAT TGTGGCCAAT 
AGTGGCAACA GTGGGAATGT TGCCCAGCAC TTGAGCGCAC GGAACACTAA TGACTATGCG 
CTGATATCGT CACAGAATTC ATCGCTGTCG CCGCATCTTG GACCCACCTG CGACGAGGTC 
GTCACGGCCA CAACATTGCC CTCGGTTAGT TTAAGCTGTG GTGGCACCGC CAGCGAAAGC 
ACCGAATATC CGCCTAGTAA AATGCATCGT AATCGTGACG TCAACCAAAT AAAACGTGAT 
TTGTCAACGG GCACCGCTCG TGTGCGCACA CTCTATGCTT GCATGGGAGA AAGCGAGGGC 
GAGCTTTCTT TCGAGCCCAA CCAAATAATA ACCAATGTTC GTTACTCCCA CGAACCCGGC 
TGGCTGCAGG GCACACTAAA TGGGAAAACA GGACTCATTC CGGAGAACTA TGTGGAACAT 
TTGAAGCCGC ACCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Graf cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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