Pis cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pis
Entrez Gene ID	32506
Full Name	Phosphatidylinositol synthase
Synonyms	CG9245,Dmel\CG9245,anon-WO02059370.38,dPIS,dpis
General protein information	Preferred Names Phosphatidylinositol synthase Names CG9245 gene product from transcript CG9245-RA CG9245-PA CG9245-PB PI synthase Phosphatidyl-inositol synthase Pis-PA Pis-PB drosophila phosphatidylinositol synthase phosphatidylinositol synthase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 13E8-13E8

mRNA and Protein(s)

mRNA	Protein	Name
NM_132827.3	NP_573055.1	phosphatidylinositol synthase, isoform A
NM_167456.3	NP_727878.1	phosphatidylinositol synthase, isoform B

Pathways from BioSystems

Homologies

	CDIPT	XP_001105279.1
Schizosaccharomyces pombe (fission yeast)	pis1	NP_593021.1
Arabidopsis thaliana (thale cress)	PIS1	NP_176967.1
Homo sapiens (human)	CDIPT	NP_006310.1
Canis lupus familiaris (dog)	CDIPT	XP_547060.4
Bos taurus (cattle)	CDIPT	NP_001029654.1
Caenorhabditis elegans (roundworm)	pisy-1	NP_496711.1
Kluyveromyces lactis	KLLA0D15037g	XP_453727.1
Eremothecium gossypii	AGOS_AAR037W	NP_982578.1
Oryza sativa (rice)	Os02g0123600	NP_001045736.1
Xenopus tropicalis (tropical clawed frog)	cdipt	NP_001072374.1
Rattus norvegicus (Norway rat)	Cdipt	NP_620254.1
Danio rerio (zebrafish)	cdipt	NP_996971.1
Neurospora crassa	NCU09192	XP_959136.1
Arabidopsis thaliana (thale cress)	PIS2	NP_001190955.1
Mus musculus (house mouse)	Cdipt	NP_080914.1
Saccharomyces cerevisiae (baker's yeast)	PIS1	NP_015438.1
Magnaporthe oryzae (rice blast fungus)	MGG_08368	XP_003715692.1
Pan troglodytes (chimpanzee)	CDIPT	XP_003952867.1
Drosophila melanogaster (fruit fly)	Pis	NP_573055.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003478	XP_311768.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Pis regulates the polarized deposition of basement membrane proteins.
Title: Polarized deposition of basement membrane proteins depends on Phosphatidylinositol synthase and the levels of Phosphatidylinositol 4,5-bisphosphate.

The following Pis gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pis cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa03581	NM_132827.3 Latest version!	Drosophila melanogaster phosphatidylinositol synthase, transcript variant A (Pis), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa03582	NM_167456.3 Latest version!	Drosophila melanogaster phosphatidylinositol synthase, transcript variant B (Pis), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pis ( NM_132827.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_573055.1 Drosophila melanogaster phosphatidylinositol synthase, transcript variant A (Pis), mRNA.

Price & Availability↑

CloneID	OFa03581
Clone ID Related Accession (Same CDS sequence)	NM_132827.3
Accession Version	NM_132827.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 675bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phosphatidylinositol synthase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_132827.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAATTG CCGAGCACGA TAACGTCTTC ATCTTTGTGC CAAATCTGAT CGGCTACGCC 
CGCATCGTTC TGGCCCTGAT CGCCTTCTGG TTCATGTCCA CCAACTATGT GATCTCCGGC 
TGGTGTTATG TAACCTCCGC CCTCCTGGAC GCCGTCGATG GACAAGCAGC CCGAGCCTTT 
AACCAAAGCA CTCGCTTTGG CGCCATGCTA GACCAGTTGA CCGATCGCTG CGGCACCACC 
GGCCTACTGG TCACCCTGGC CTACTTCTAT CCCCGGTACA TGTTCTGGTT CCAGCTGTCC 
ATCGCCATCG ACGTGGCCTG TCACTGGCTC TTCATGCAGA CGAGCGTTGT GGTCGGACGC 
AGCTCGCACA AGGTGAATGA CAACTTCATA ATGCGATTGT ATTACCAGAA GGACATACTC 
ACATTCATGT GCTGCGTCAA TGAGCTGTTT TACGTGTGCT TGTACCTCTT GCATTTCACC 
TACGGCCCGC TGATATTCGG CGCATCGCTC TTCAAGATAC TCGCCTTCCT CACGGGTCCC 
TTTGCCGTGC TCAAGGCCCT GATCTCGGTG ATGCACGCCT ACGTGGCGGG CATCGATCTG 
GCGGCCGTGG ATGTACGCGA GCGGCAGGAG CGGCGCCAGA AGTCGGAGCC GGTGAGCGGC 
AAGAAAGTGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_573055.1
CDS	150..824
Translation	MTIAEHDNVFIFVPNLIGYARIVLALIAFWFMSTNYVISGWCYVTSALLDAVDGQAARAFNQSTRFGAMLDQLTDRCGTTGLLVTLAYFYPRYMFWFQLSIAIDVACHWLFMQTSVVVGRSSHKVNDNFIMRLYYQKDILTFMCCVNELFYVCLYLLHFTYGPLIFGASLFKILAFLTGPFAVLKALISVMHAYVAGIDLAAVDVRERQERRQKSEPVSGKKVE

Target ORF information:

RefSeq Version	NM_132827.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phosphatidylinositol synthase, transcript variant A (Pis), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132827.3

ORF Insert Sequence:

ATGACAATTG CCGAGCACGA TAACGTCTTC ATCTTTGTGC CAAATCTGAT CGGCTACGCC 
CGCATCGTTC TGGCCCTGAT CGCCTTCTGG TTCATGTCCA CCAACTATGT GATCTCCGGC 
TGGTGTTATG TAACCTCCGC CCTCCTGGAC GCCGTCGATG GACAAGCAGC CCGAGCCTTT 
AACCAAAGCA CTCGCTTTGG CGCCATGCTA GACCAGTTGA CCGATCGCTG CGGCACCACC 
GGCCTACTGG TCACCCTGGC CTACTTCTAT CCCCGGTACA TGTTCTGGTT CCAGCTGTCC 
ATCGCCATCG ACGTGGCCTG TCACTGGCTC TTCATGCAGA CGAGCGTTGT GGTCGGACGC 
AGCTCGCACA AGGTGAATGA CAACTTCATA ATGCGATTGT ATTACCAGAA GGACATACTC 
ACATTCATGT GCTGCGTCAA TGAGCTGTTT TACGTGTGCT TGTACCTCTT GCATTTCACC 
TACGGCCCGC TGATATTCGG CGCATCGCTC TTCAAGATAC TCGCCTTCCT CACGGGTCCC 
TTTGCCGTGC TCAAGGCCCT GATCTCGGTG ATGCACGCCT ACGTGGCGGG CATCGATCTG 
GCGGCCGTGG ATGTACGCGA GCGGCAGGAG CGGCGCCAGA AGTCGGAGCC GGTGAGCGGC 
AAGAAAGTGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pis ( NM_167456.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727878.1 Drosophila melanogaster phosphatidylinositol synthase, transcript variant B (Pis), mRNA.

Price & Availability↑

CloneID	OFa03582
Clone ID Related Accession (Same CDS sequence)	NM_167456.3
Accession Version	NM_167456.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phosphatidylinositol synthase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167456.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAATTG CCGAGCACGA TAACGTCTTC ATCTTTGTGC CAAATCTGAT CGGCTACGCC 
CGCATCGTTC TGGCCCTGAT CGCCTTCTGG TTCATGTCCA CCAACTATGT GATCTCCGGC 
TGGTGTTATG TAACCTCCGC CCTCCTGGAC GCCGTCGATG GACAAGCAGC CCGAGCCTTT 
AACCAAACGC ATTGCAAACA CCTGTCTCCT CCGTACTGGT TATCTGCCGA TATTGTGGAA 
TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727878.1
CDS	150..395
Translation	MTIAEHDNVFIFVPNLIGYARIVLALIAFWFMSTNYVISGWCYVTSALLDAVDGQAARAFNQTHCKHLSPPYWLSADIVEL

Target ORF information:

RefSeq Version	NM_167456.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phosphatidylinositol synthase, transcript variant B (Pis), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167456.3

ORF Insert Sequence:

ATGACAATTG CCGAGCACGA TAACGTCTTC ATCTTTGTGC CAAATCTGAT CGGCTACGCC 
CGCATCGTTC TGGCCCTGAT CGCCTTCTGG TTCATGTCCA CCAACTATGT GATCTCCGGC 
TGGTGTTATG TAACCTCCGC CCTCCTGGAC GCCGTCGATG GACAAGCAGC CCGAGCCTTT 
AACCAAACGC ATTGCAAACA CCTGTCTCCT CCGTACTGGT TATCTGCCGA TATTGTGGAA 
TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Pis cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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