CG9220 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG9220
Entrez Gene ID	32497
Full Name	CG9220 gene product from transcript CG9220-RC
Synonyms	Dmel\CG9220,dCHSY
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 13D4-13E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_206717.3	NP_996440.2	uncharacterized protein, isoform C
NM_001298351.1	NP_001285280.1	uncharacterized protein, isoform D

Pathways from BioSystems

Homologies

	CHSY1	XP_001084554.2
Rattus norvegicus (Norway rat)	Chsy1	NP_001099738.1
Homo sapiens (human)	CHSY1	NP_055733.2
Gallus gallus (chicken)	CHSY1	XP_413889.3
Drosophila melanogaster (fruit fly)	CG9220	NP_996440.2
Canis lupus familiaris (dog)	CHSY1	XP_545821.3
Danio rerio (zebrafish)	chsy1	NP_997843.1
Bos taurus (cattle)	CHSY1	NP_001178086.1
Mus musculus (house mouse)	Chsy1	NP_001074632.1
Xenopus tropicalis (tropical clawed frog)	chsy1	NP_001090724.1
Pan troglodytes (chimpanzee)	CHSY1	XP_523172.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001010	XP_309203.5

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG9220 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG9220 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa01712	NM_206717.3 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant C (CG9220), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa01712	NM_001298351.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant D (CG9220), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG9220 ( NM_206717.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996440.2 Drosophila melanogaster uncharacterized protein, transcript variant C (CG9220), mRNA.

Price & Availability↑

CloneID	OFa01712
Clone ID Related Accession (Same CDS sequence)	NM_206717.3 , NM_001298351.1
Accession Version	NM_206717.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2499bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_206717.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTGCAGC ACACGATGAC GAAGCGGAAG ACGCTGATCA TCGGCTTCTT TGGCATCGCC 
CTGGGCCTCT GCATCGGCAC CATGCTGAAG AACTACCTGG CGCTGGAGAT CGTGAAGCGA 
TGCAGCCTGC GCCCGACGAA TCTCAAGACG CCGGCGGATA TCATTGGACT GCGGGATGAG 
GACACCATAC AGAATTCGCA GCGGAATCTA GTCTTTGTGG GCGTGATGAC GGCGAAAAGC 
TTCCTGGAGG GCAGAGCGCG AGCGGTGTAC GATACGTGGG GCAAGGAGGT GCCCGGCCGG 
ATGGCCTTCT TCAGTTCGGA GGGCAGCTAC TCGGATGACC TGCCCGTGGT GGGGCTAAAG 
AACGTGGACG ATCGCTATCC GCCGCAGAAG AAGTCCTTCA TGATGCTGTA CTACATGTAC 
GAGCACTACA TCGACCGCTT CGAGTGGTTC ATCCGGGCGG ACGACGATGT CTACATGGAG 
CCGGACAAGC TGGAGCGCTT CCTGCGCTCC ATCGACAGCT CCAAGCCGCA GTTCATCGGC 
CAGGCGGGCA AGGGCAATAG CGAGGAGTTC GGCCTGCTGT CTCTCGAGTT CGACGAGAAC 
TTCTGCATGG GCGGACCGGG GGTGATCCTC AGCAGTGAGA CTCTGCGCCG CGTGGCGCCA 
CACATACCCA GCTGCCTGAA GAACCTCTAC AGCACCCACG AGGACGTCGA GGTGGGGCGG 
TGTGTCCAGA AGTTCGCCGG CATACCCTGC ACTTGGAACT ACGAGATGCA GTACATTTTG 
CGGCACAACT CCAGTGGACG AAATGCGTAC ACGGGCAAAC TGAAGCGCAA GGAGATCCAC 
AACGCCATCA CCCTGCATCC CATCAAACAG GCGCCGCTCA TGTACCGCCT GCACTCCTAT 
GTCCAGGGTT TGAAAGCGGA GGAGATGCGT CAGGAGTCGC TGCTGCTGCA TCGGGATATC 
AAGCGGATGG CCAAATATCT GGAGGTTCCG GACGAGAGCA CCTACATGTT GCCCAGTGTG 
TCACCCGAAA GCGATTCCAC CAAAAGGCAC TTCCAGGATC ACAACATATT AGGAATCAGT 
CCGGAACTGA ATAAATTCGT GCCCGCAAGC ACCGACGACC TGCTGGACTG GTCCTTCATC 
GCCCGCAGCC TGTACTCGGC AAGTTCCGCC AATCCCAAGC AGAAGATCGA CTCGGCGATG 
CGCGAGGGAC TGGAGGATGC CATCACCGAG GTGATGGAGA ACATCAACAA CTATTCCAGG 
CAACGGGGTC GCGTCATCGA GTTCCGGGAA CTGCTCTACG GATACCATCG GCTGGACGCC 
CTCCACGGCC AGGACATGAT CCTCGATCTG CTGCTCATCT ACAAGAAGTA CCGGGGCAAG 
AAGATGACCG TTCCGGTGCG AAGGCATCTT TATGTTCAGC GCGCCTTTAC GGGCATCTTT 
GTCAAGGAGG TGGACGAGGA CTTCTACAAT GTCACCCTGC AGCAAAGTCT CCTGGGCAGC 
CTGTTCCAGA ACGGAATGGC CCGCTTGAGC AGCCACTTCA CGATGCCGAG TGGACTGCTG 
TCACCGACGC AGGACAAGAT CGTGTTCGTC CTGCCCATCG CCGGTCGTTT GGGTACATTC 
GAGCGCTTCC TGCGCACCTA CGAGCGCGTC TGCGTGCGTG GCGAACAGCA CTGCGACCTC 
CTGGTGGTGA TCTTCGGGTC ACCGGACGAG CTGGGCGATC ACCTGCAGCT GCTCCATGAT 
CTGCACGCCC GGCATGTCTA CCAGCAGGTG AACTGGATTC AAAGAAGCAG CGCCTTTTCC 
CGTGGCGTTG CCCTGGACGT GGCCGCCAGA TCCTCGTACA TTCGTCAGGA GGACATCATT 
CTGTTTATCG ACGTGGACAT GGTCTTCGAG GTGGAGACGC TGCAGCGCGT GAGGATGCAC 
ACGCAGCGGG GCAAACAGGT TTACCTCCCC ATCGTCTTCA GTCAGTATGA TCCGCAGAGG 
AGAAGCGGTG ACGCTGGGGG ATCCGAGGAC GAGGGAGAAA CCCCCAGGAT CGACGACGAA 
CGTGGCTATT TCCGGCAATT TGGGTTCGGC ATCTGTGCCA TCTACAAGTC GGACATACTC 
GATGAGGATA TCAACGGATT CGATAAGGAC ATCACCGGCT GGGGCCTGGA GGACGTCAAA 
TTCCTGGAGA AGATCGTGCG CGTAGGCACC AGGCAACGGG GCTTCCTAGC CAACACCGCC 
GAACTGGCCA TGGACTACAA CGAGGCCGCT GAGCAGTGGC GCAGATTGAG TGTCTTCCGT 
GCACCGGATC CAACGCTGGT CCACATTTAC CATGACATCA GCTGCGACGT GCAGCTGGAT 
GCGCCGCAGT ACAACATGTG CCTGGGCACC AAGGCAAACT CCCTGGGCAG CACGCGGCTA 
ATGGAGCAGC TCTTCCACAG CAGTCCCGAA AATGTCCAGT TTGCCGCCGA TTTCAATCGC 
CAAAAGCAAC AGCAGCAACA GCAACAGCAG GCCAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996440.2
CDS	709..3207
Translation	MLQHTMTKRKTLIIGFFGIALGLCIGTMLKNYLALEIVKRCSLRPTNLKTPADIIGLRDEDTIQNSQRNLVFVGVMTAKSFLEGRARAVYDTWGKEVPGRMAFFSSEGSYSDDLPVVGLKNVDDRYPPQKKSFMMLYYMYEHYIDRFEWFIRADDDVYMEPDKLERFLRSIDSSKPQFIGQAGKGNSEEFGLLSLEFDENFCMGGPGVILSSETLRRVAPHIPSCLKNLYSTHEDVEVGRCVQKFAGIPCTWNYEMQYILRHNSSGRNAYTGKLKRKEIHNAITLHPIKQAPLMYRLHSYVQGLKAEEMRQESLLLHRDIKRMAKYLEVPDESTYMLPSVSPESDSTKRHFQDHNILGISPELNKFVPASTDDLLDWSFIARSLYSASSANPKQKIDSAMREGLEDAITEVMENINNYSRQRGRVIEFRELLYGYHRLDALHGQDMILDLLLIYKKYRGKKMTVPVRRHLYVQRAFTGIFVKEVDEDFYNVTLQQSLLGSLFQNGMARLSSHFTMPSGLLSPTQDKIVFVLPIAGRLGTFERFLRTYERVCVRGEQHCDLLVVIFGSPDELGDHLQLLHDLHARHVYQQVNWIQRSSAFSRGVALDVAARSSYIRQEDIILFIDVDMVFEVETLQRVRMHTQRGKQVYLPIVFSQYDPQRRSGDAGGSEDEGETPRIDDERGYFRQFGFGICAIYKSDILDEDINGFDKDITGWGLEDVKFLEKIVRVGTRQRGFLANTAELAMDYNEAAEQWRRLSVFRAPDPTLVHIYHDISCDVQLDAPQYNMCLGTKANSLGSTRLMEQLFHSSPENVQFAADFNRQKQQQQQQQQAR

Target ORF information:

RefSeq Version	NM_206717.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant C (CG9220), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206717.3

ORF Insert Sequence:

ATGCTGCAGC ACACGATGAC GAAGCGGAAG ACGCTGATCA TCGGCTTCTT TGGCATCGCC 
CTGGGCCTCT GCATCGGCAC CATGCTGAAG AACTACCTGG CGCTGGAGAT CGTGAAGCGA 
TGCAGCCTGC GCCCGACGAA TCTCAAGACG CCGGCGGATA TCATTGGACT GCGGGATGAG 
GACACCATAC AGAATTCGCA GCGGAATCTA GTCTTTGTGG GCGTGATGAC GGCGAAAAGC 
TTCCTGGAGG GCAGAGCGCG AGCGGTGTAC GATACGTGGG GCAAGGAGGT GCCCGGCCGG 
ATGGCCTTCT TCAGTTCGGA GGGCAGCTAC TCGGATGACC TGCCCGTGGT GGGGCTAAAG 
AACGTGGACG ATCGCTATCC GCCGCAGAAG AAGTCCTTCA TGATGCTGTA CTACATGTAC 
GAGCACTACA TCGACCGCTT CGAGTGGTTC ATCCGGGCGG ACGACGATGT CTACATGGAG 
CCGGACAAGC TGGAGCGCTT CCTGCGCTCC ATCGACAGCT CCAAGCCGCA GTTCATCGGC 
CAGGCGGGCA AGGGCAATAG CGAGGAGTTC GGCCTGCTGT CTCTCGAGTT CGACGAGAAC 
TTCTGCATGG GCGGACCGGG GGTGATCCTC AGCAGTGAGA CTCTGCGCCG CGTGGCGCCA 
CACATACCCA GCTGCCTGAA GAACCTCTAC AGCACCCACG AGGACGTCGA GGTGGGGCGG 
TGTGTCCAGA AGTTCGCCGG CATACCCTGC ACTTGGAACT ACGAGATGCA GTACATTTTG 
CGGCACAACT CCAGTGGACG AAATGCGTAC ACGGGCAAAC TGAAGCGCAA GGAGATCCAC 
AACGCCATCA CCCTGCATCC CATCAAACAG GCGCCGCTCA TGTACCGCCT GCACTCCTAT 
GTCCAGGGTT TGAAAGCGGA GGAGATGCGT CAGGAGTCGC TGCTGCTGCA TCGGGATATC 
AAGCGGATGG CCAAATATCT GGAGGTTCCG GACGAGAGCA CCTACATGTT GCCCAGTGTG 
TCACCCGAAA GCGATTCCAC CAAAAGGCAC TTCCAGGATC ACAACATATT AGGAATCAGT 
CCGGAACTGA ATAAATTCGT GCCCGCAAGC ACCGACGACC TGCTGGACTG GTCCTTCATC 
GCCCGCAGCC TGTACTCGGC AAGTTCCGCC AATCCCAAGC AGAAGATCGA CTCGGCGATG 
CGCGAGGGAC TGGAGGATGC CATCACCGAG GTGATGGAGA ACATCAACAA CTATTCCAGG 
CAACGGGGTC GCGTCATCGA GTTCCGGGAA CTGCTCTACG GATACCATCG GCTGGACGCC 
CTCCACGGCC AGGACATGAT CCTCGATCTG CTGCTCATCT ACAAGAAGTA CCGGGGCAAG 
AAGATGACCG TTCCGGTGCG AAGGCATCTT TATGTTCAGC GCGCCTTTAC GGGCATCTTT 
GTCAAGGAGG TGGACGAGGA CTTCTACAAT GTCACCCTGC AGCAAAGTCT CCTGGGCAGC 
CTGTTCCAGA ACGGAATGGC CCGCTTGAGC AGCCACTTCA CGATGCCGAG TGGACTGCTG 
TCACCGACGC AGGACAAGAT CGTGTTCGTC CTGCCCATCG CCGGTCGTTT GGGTACATTC 
GAGCGCTTCC TGCGCACCTA CGAGCGCGTC TGCGTGCGTG GCGAACAGCA CTGCGACCTC 
CTGGTGGTGA TCTTCGGGTC ACCGGACGAG CTGGGCGATC ACCTGCAGCT GCTCCATGAT 
CTGCACGCCC GGCATGTCTA CCAGCAGGTG AACTGGATTC AAAGAAGCAG CGCCTTTTCC 
CGTGGCGTTG CCCTGGACGT GGCCGCCAGA TCCTCGTACA TTCGTCAGGA GGACATCATT 
CTGTTTATCG ACGTGGACAT GGTCTTCGAG GTGGAGACGC TGCAGCGCGT GAGGATGCAC 
ACGCAGCGGG GCAAACAGGT TTACCTCCCC ATCGTCTTCA GTCAGTATGA TCCGCAGAGG 
AGAAGCGGTG ACGCTGGGGG ATCCGAGGAC GAGGGAGAAA CCCCCAGGAT CGACGACGAA 
CGTGGCTATT TCCGGCAATT TGGGTTCGGC ATCTGTGCCA TCTACAAGTC GGACATACTC 
GATGAGGATA TCAACGGATT CGATAAGGAC ATCACCGGCT GGGGCCTGGA GGACGTCAAA 
TTCCTGGAGA AGATCGTGCG CGTAGGCACC AGGCAACGGG GCTTCCTAGC CAACACCGCC 
GAACTGGCCA TGGACTACAA CGAGGCCGCT GAGCAGTGGC GCAGATTGAG TGTCTTCCGT 
GCACCGGATC CAACGCTGGT CCACATTTAC CATGACATCA GCTGCGACGT GCAGCTGGAT 
GCGCCGCAGT ACAACATGTG CCTGGGCACC AAGGCAAACT CCCTGGGCAG CACGCGGCTA 
ATGGAGCAGC TCTTCCACAG CAGTCCCGAA AATGTCCAGT TTGCCGCCGA TTTCAATCGC 
CAAAAGCAAC AGCAGCAACA GCAACAGCAG GCCAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG9220 ( NM_001298351.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285280.1 Drosophila melanogaster uncharacterized protein, transcript variant D (CG9220), mRNA.

Price & Availability↑

CloneID	OFa01712
Clone ID Related Accession (Same CDS sequence)	NM_206717.3 , NM_001298351.1
Accession Version	NM_001298351.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2499bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTGCAGC ACACGATGAC GAAGCGGAAG ACGCTGATCA TCGGCTTCTT TGGCATCGCC 
CTGGGCCTCT GCATCGGCAC CATGCTGAAG AACTACCTGG CGCTGGAGAT CGTGAAGCGA 
TGCAGCCTGC GCCCGACGAA TCTCAAGACG CCGGCGGATA TCATTGGACT GCGGGATGAG 
GACACCATAC AGAATTCGCA GCGGAATCTA GTCTTTGTGG GCGTGATGAC GGCGAAAAGC 
TTCCTGGAGG GCAGAGCGCG AGCGGTGTAC GATACGTGGG GCAAGGAGGT GCCCGGCCGG 
ATGGCCTTCT TCAGTTCGGA GGGCAGCTAC TCGGATGACC TGCCCGTGGT GGGGCTAAAG 
AACGTGGACG ATCGCTATCC GCCGCAGAAG AAGTCCTTCA TGATGCTGTA CTACATGTAC 
GAGCACTACA TCGACCGCTT CGAGTGGTTC ATCCGGGCGG ACGACGATGT CTACATGGAG 
CCGGACAAGC TGGAGCGCTT CCTGCGCTCC ATCGACAGCT CCAAGCCGCA GTTCATCGGC 
CAGGCGGGCA AGGGCAATAG CGAGGAGTTC GGCCTGCTGT CTCTCGAGTT CGACGAGAAC 
TTCTGCATGG GCGGACCGGG GGTGATCCTC AGCAGTGAGA CTCTGCGCCG CGTGGCGCCA 
CACATACCCA GCTGCCTGAA GAACCTCTAC AGCACCCACG AGGACGTCGA GGTGGGGCGG 
TGTGTCCAGA AGTTCGCCGG CATACCCTGC ACTTGGAACT ACGAGATGCA GTACATTTTG 
CGGCACAACT CCAGTGGACG AAATGCGTAC ACGGGCAAAC TGAAGCGCAA GGAGATCCAC 
AACGCCATCA CCCTGCATCC CATCAAACAG GCGCCGCTCA TGTACCGCCT GCACTCCTAT 
GTCCAGGGTT TGAAAGCGGA GGAGATGCGT CAGGAGTCGC TGCTGCTGCA TCGGGATATC 
AAGCGGATGG CCAAATATCT GGAGGTTCCG GACGAGAGCA CCTACATGTT GCCCAGTGTG 
TCACCCGAAA GCGATTCCAC CAAAAGGCAC TTCCAGGATC ACAACATATT AGGAATCAGT 
CCGGAACTGA ATAAATTCGT GCCCGCAAGC ACCGACGACC TGCTGGACTG GTCCTTCATC 
GCCCGCAGCC TGTACTCGGC AAGTTCCGCC AATCCCAAGC AGAAGATCGA CTCGGCGATG 
CGCGAGGGAC TGGAGGATGC CATCACCGAG GTGATGGAGA ACATCAACAA CTATTCCAGG 
CAACGGGGTC GCGTCATCGA GTTCCGGGAA CTGCTCTACG GATACCATCG GCTGGACGCC 
CTCCACGGCC AGGACATGAT CCTCGATCTG CTGCTCATCT ACAAGAAGTA CCGGGGCAAG 
AAGATGACCG TTCCGGTGCG AAGGCATCTT TATGTTCAGC GCGCCTTTAC GGGCATCTTT 
GTCAAGGAGG TGGACGAGGA CTTCTACAAT GTCACCCTGC AGCAAAGTCT CCTGGGCAGC 
CTGTTCCAGA ACGGAATGGC CCGCTTGAGC AGCCACTTCA CGATGCCGAG TGGACTGCTG 
TCACCGACGC AGGACAAGAT CGTGTTCGTC CTGCCCATCG CCGGTCGTTT GGGTACATTC 
GAGCGCTTCC TGCGCACCTA CGAGCGCGTC TGCGTGCGTG GCGAACAGCA CTGCGACCTC 
CTGGTGGTGA TCTTCGGGTC ACCGGACGAG CTGGGCGATC ACCTGCAGCT GCTCCATGAT 
CTGCACGCCC GGCATGTCTA CCAGCAGGTG AACTGGATTC AAAGAAGCAG CGCCTTTTCC 
CGTGGCGTTG CCCTGGACGT GGCCGCCAGA TCCTCGTACA TTCGTCAGGA GGACATCATT 
CTGTTTATCG ACGTGGACAT GGTCTTCGAG GTGGAGACGC TGCAGCGCGT GAGGATGCAC 
ACGCAGCGGG GCAAACAGGT TTACCTCCCC ATCGTCTTCA GTCAGTATGA TCCGCAGAGG 
AGAAGCGGTG ACGCTGGGGG ATCCGAGGAC GAGGGAGAAA CCCCCAGGAT CGACGACGAA 
CGTGGCTATT TCCGGCAATT TGGGTTCGGC ATCTGTGCCA TCTACAAGTC GGACATACTC 
GATGAGGATA TCAACGGATT CGATAAGGAC ATCACCGGCT GGGGCCTGGA GGACGTCAAA 
TTCCTGGAGA AGATCGTGCG CGTAGGCACC AGGCAACGGG GCTTCCTAGC CAACACCGCC 
GAACTGGCCA TGGACTACAA CGAGGCCGCT GAGCAGTGGC GCAGATTGAG TGTCTTCCGT 
GCACCGGATC CAACGCTGGT CCACATTTAC CATGACATCA GCTGCGACGT GCAGCTGGAT 
GCGCCGCAGT ACAACATGTG CCTGGGCACC AAGGCAAACT CCCTGGGCAG CACGCGGCTA 
ATGGAGCAGC TCTTCCACAG CAGTCCCGAA AATGTCCAGT TTGCCGCCGA TTTCAATCGC 
CAAAAGCAAC AGCAGCAACA GCAACAGCAG GCCAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285280.1
CDS	326..2824
Translation	MLQHTMTKRKTLIIGFFGIALGLCIGTMLKNYLALEIVKRCSLRPTNLKTPADIIGLRDEDTIQNSQRNLVFVGVMTAKSFLEGRARAVYDTWGKEVPGRMAFFSSEGSYSDDLPVVGLKNVDDRYPPQKKSFMMLYYMYEHYIDRFEWFIRADDDVYMEPDKLERFLRSIDSSKPQFIGQAGKGNSEEFGLLSLEFDENFCMGGPGVILSSETLRRVAPHIPSCLKNLYSTHEDVEVGRCVQKFAGIPCTWNYEMQYILRHNSSGRNAYTGKLKRKEIHNAITLHPIKQAPLMYRLHSYVQGLKAEEMRQESLLLHRDIKRMAKYLEVPDESTYMLPSVSPESDSTKRHFQDHNILGISPELNKFVPASTDDLLDWSFIARSLYSASSANPKQKIDSAMREGLEDAITEVMENINNYSRQRGRVIEFRELLYGYHRLDALHGQDMILDLLLIYKKYRGKKMTVPVRRHLYVQRAFTGIFVKEVDEDFYNVTLQQSLLGSLFQNGMARLSSHFTMPSGLLSPTQDKIVFVLPIAGRLGTFERFLRTYERVCVRGEQHCDLLVVIFGSPDELGDHLQLLHDLHARHVYQQVNWIQRSSAFSRGVALDVAARSSYIRQEDIILFIDVDMVFEVETLQRVRMHTQRGKQVYLPIVFSQYDPQRRSGDAGGSEDEGETPRIDDERGYFRQFGFGICAIYKSDILDEDINGFDKDITGWGLEDVKFLEKIVRVGTRQRGFLANTAELAMDYNEAAEQWRRLSVFRAPDPTLVHIYHDISCDVQLDAPQYNMCLGTKANSLGSTRLMEQLFHSSPENVQFAADFNRQKQQQQQQQQAR

Target ORF information:

RefSeq Version	NM_001298351.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant D (CG9220), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298351.1

ORF Insert Sequence:

ATGCTGCAGC ACACGATGAC GAAGCGGAAG ACGCTGATCA TCGGCTTCTT TGGCATCGCC 
CTGGGCCTCT GCATCGGCAC CATGCTGAAG AACTACCTGG CGCTGGAGAT CGTGAAGCGA 
TGCAGCCTGC GCCCGACGAA TCTCAAGACG CCGGCGGATA TCATTGGACT GCGGGATGAG 
GACACCATAC AGAATTCGCA GCGGAATCTA GTCTTTGTGG GCGTGATGAC GGCGAAAAGC 
TTCCTGGAGG GCAGAGCGCG AGCGGTGTAC GATACGTGGG GCAAGGAGGT GCCCGGCCGG 
ATGGCCTTCT TCAGTTCGGA GGGCAGCTAC TCGGATGACC TGCCCGTGGT GGGGCTAAAG 
AACGTGGACG ATCGCTATCC GCCGCAGAAG AAGTCCTTCA TGATGCTGTA CTACATGTAC 
GAGCACTACA TCGACCGCTT CGAGTGGTTC ATCCGGGCGG ACGACGATGT CTACATGGAG 
CCGGACAAGC TGGAGCGCTT CCTGCGCTCC ATCGACAGCT CCAAGCCGCA GTTCATCGGC 
CAGGCGGGCA AGGGCAATAG CGAGGAGTTC GGCCTGCTGT CTCTCGAGTT CGACGAGAAC 
TTCTGCATGG GCGGACCGGG GGTGATCCTC AGCAGTGAGA CTCTGCGCCG CGTGGCGCCA 
CACATACCCA GCTGCCTGAA GAACCTCTAC AGCACCCACG AGGACGTCGA GGTGGGGCGG 
TGTGTCCAGA AGTTCGCCGG CATACCCTGC ACTTGGAACT ACGAGATGCA GTACATTTTG 
CGGCACAACT CCAGTGGACG AAATGCGTAC ACGGGCAAAC TGAAGCGCAA GGAGATCCAC 
AACGCCATCA CCCTGCATCC CATCAAACAG GCGCCGCTCA TGTACCGCCT GCACTCCTAT 
GTCCAGGGTT TGAAAGCGGA GGAGATGCGT CAGGAGTCGC TGCTGCTGCA TCGGGATATC 
AAGCGGATGG CCAAATATCT GGAGGTTCCG GACGAGAGCA CCTACATGTT GCCCAGTGTG 
TCACCCGAAA GCGATTCCAC CAAAAGGCAC TTCCAGGATC ACAACATATT AGGAATCAGT 
CCGGAACTGA ATAAATTCGT GCCCGCAAGC ACCGACGACC TGCTGGACTG GTCCTTCATC 
GCCCGCAGCC TGTACTCGGC AAGTTCCGCC AATCCCAAGC AGAAGATCGA CTCGGCGATG 
CGCGAGGGAC TGGAGGATGC CATCACCGAG GTGATGGAGA ACATCAACAA CTATTCCAGG 
CAACGGGGTC GCGTCATCGA GTTCCGGGAA CTGCTCTACG GATACCATCG GCTGGACGCC 
CTCCACGGCC AGGACATGAT CCTCGATCTG CTGCTCATCT ACAAGAAGTA CCGGGGCAAG 
AAGATGACCG TTCCGGTGCG AAGGCATCTT TATGTTCAGC GCGCCTTTAC GGGCATCTTT 
GTCAAGGAGG TGGACGAGGA CTTCTACAAT GTCACCCTGC AGCAAAGTCT CCTGGGCAGC 
CTGTTCCAGA ACGGAATGGC CCGCTTGAGC AGCCACTTCA CGATGCCGAG TGGACTGCTG 
TCACCGACGC AGGACAAGAT CGTGTTCGTC CTGCCCATCG CCGGTCGTTT GGGTACATTC 
GAGCGCTTCC TGCGCACCTA CGAGCGCGTC TGCGTGCGTG GCGAACAGCA CTGCGACCTC 
CTGGTGGTGA TCTTCGGGTC ACCGGACGAG CTGGGCGATC ACCTGCAGCT GCTCCATGAT 
CTGCACGCCC GGCATGTCTA CCAGCAGGTG AACTGGATTC AAAGAAGCAG CGCCTTTTCC 
CGTGGCGTTG CCCTGGACGT GGCCGCCAGA TCCTCGTACA TTCGTCAGGA GGACATCATT 
CTGTTTATCG ACGTGGACAT GGTCTTCGAG GTGGAGACGC TGCAGCGCGT GAGGATGCAC 
ACGCAGCGGG GCAAACAGGT TTACCTCCCC ATCGTCTTCA GTCAGTATGA TCCGCAGAGG 
AGAAGCGGTG ACGCTGGGGG ATCCGAGGAC GAGGGAGAAA CCCCCAGGAT CGACGACGAA 
CGTGGCTATT TCCGGCAATT TGGGTTCGGC ATCTGTGCCA TCTACAAGTC GGACATACTC 
GATGAGGATA TCAACGGATT CGATAAGGAC ATCACCGGCT GGGGCCTGGA GGACGTCAAA 
TTCCTGGAGA AGATCGTGCG CGTAGGCACC AGGCAACGGG GCTTCCTAGC CAACACCGCC 
GAACTGGCCA TGGACTACAA CGAGGCCGCT GAGCAGTGGC GCAGATTGAG TGTCTTCCGT 
GCACCGGATC CAACGCTGGT CCACATTTAC CATGACATCA GCTGCGACGT GCAGCTGGAT 
GCGCCGCAGT ACAACATGTG CCTGGGCACC AAGGCAAACT CCCTGGGCAG CACGCGGCTA 
ATGGAGCAGC TCTTCCACAG CAGTCCCGAA AATGTCCAGT TTGCCGCCGA TTTCAATCGC 
CAAAAGCAAC AGCAGCAACA GCAACAGCAG GCCAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG9220 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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