shtd cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	shtd
Entrez Gene ID	32473
Full Name	shattered
Synonyms	APC1,Apc1,CG9198,Dmel\CG9198,SHTD,Shtd
General protein information	Preferred Names shattered Names CG9198 gene product from transcript CG9198-RA CG9198-PA shtd-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 13C3-13C4\|1-53.6 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_132797.3	NP_573025.2	shattered

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ANAPC1	XP_005630492.1
Rattus norvegicus (Norway rat)	Anapc1	NP_001101241.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000374	XP_310737.5
Arabidopsis thaliana (thale cress)	EMB2771	NP_196175.1
Oryza sativa (rice)	Os05g0354300	NP_001055285.1
Drosophila melanogaster (fruit fly)	shtd	NP_573025.2
Xenopus tropicalis (tropical clawed frog)	anapc1	NP_001072866.1
Homo sapiens (human)	ANAPC1	NP_073153.1
Mus musculus (house mouse)	Anapc1	NP_032595.2
Danio rerio (zebrafish)	anapc1	XP_001921168.2
Bos taurus (cattle)	ANAPC1	XP_005212426.1
Gallus gallus (chicken)	ANAPC1	XP_419303.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

shtd encodes Apc1; reducing the dosage of either CycA or stg suppressed the shtd1 phenotype.
Title: Mutation of the Apc1 homologue shattered disrupts normal eye development by disrupting G1 cell cycle arrest and progression through mitosis.

The following shtd gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the shtd cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa01685	NM_132797.3 Latest version!	Drosophila melanogaster shattered (shtd), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

shtd ( NM_132797.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_573025.2 Drosophila melanogaster shattered (shtd), mRNA.

Price & Availability↑

CloneID	OFa01685
Clone ID Related Accession (Same CDS sequence)	NM_132797.3
Accession Version	NM_132797.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6093bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	shattered
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_132797.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATCGCCG TCGCAGAACC GCCGCTGGAG TTTGTGCCGC GCGGCCGTCA GACGTCCGCG 
GAGCATCCGG GCCCACATGA CCAGCCAATG GCGCGCCACC AACTGCCCAC CACGGAGCAT 
CTGCTGCTGC AGCGCCTCCA GAATGTGAAC ATCTCGTCGG CCGGTGAGGA CTGCCCTTCG 
GGCGGACAGG AGTCGTGGAC AGTCCGAGAG CTGTACGATG ACTACGAGGC ATCCGAGGAG 
CGCGCCGCCC TGCGACGCAA GTTGACCGAG CGGACAAAGC GATGTGGTCT CCAGGCCACC 
TCGCTGCCGA ACAGACTGCT CAATCCACCG CTGGTGCGGC GCGTGGAGCC AATGTGCGAC 
TATATGGTTA ACAACGAGGA GGAGCTCTAT GTGAACCGGA ACACGGTGGT GTGGACACAA 
GGCGTCAACG ATGACGACGA TACCGACAAT GGGGTGTACC GGCGCATGTG TTTCACCACC 
GATACGCCGG TGCGCTTCGC CTGCTTCCTG AATCGCAGTT TTGTGCGCGG GCGACTGGCG 
CAGCTGAAGG CGGCTGTCCA CCCGGATGAC GACCATTTGA CCGCCATCTG TGTGATGGAT 
CAGGACGCCC TGCGGGTGTA CTGCAGCAAC GGCGAGGACT TTCTAGCCAA TCTGGACTTT 
CCCGTGTCAC AGCTGTGGCA AACCAAGTAT GGCCTGCTGC TGGAAAAGGA CTCCAGCAAT 
GCCTTGATAT CGCACATGTC CATTCCGATG CCGCGTCTCT TCTCTATGTC GCATCCGCTG 
CACGAGGCCT GTCCCGTCGT CCTGAAGACG GCCACCGGCT CCACCGGCTA CATGACCGAA 
CCGGAATACA CTGTGGTCTT CACCACCGAG GAATCGGATT TGGTGATGCT GTACGATGCC 
AAGTTCTTTA AGCATTTCGT TGCACGCCTG CGTAAGGTAA CGCCCGAAGA GATCAACTAT 
GTGAGTCAGC AGATGGAGCT GGGACAGACG CTAATGGGAC CCAGGTCGAT GGCAGGCAAT 
AGTTTCAGTT CCACCAAGCA AACTGGAGCC ACGCCAAAAG CAACGAACTT ATCGTTTGCG 
GCCAGGAATA TCAACACCAC CACCACCGGC ATGGGCAACC AGTTCGGACT CAGCCAGTCG 
CAGTCGTTCA GCGGAGTTCT TGGACAGTCG AATCGCGCCT CCCTAGGCAC TCCATTGAGC 
CAACTGCAGA GCAGTATTAG CCAGCAGTCC ATGTCCGTGA AGGATATGCG CAAGTTAACG 
CACGTGAAGC CGGCAAAACC CATTGAGCCG GAATTGTGTA TGGAGCACAT CTGGACGGAG 
AACATCTACG GAACTCAACG AGAGTTCTGC GAGATGGCCA CCCGAGCATT TATCCACACG 
GATTTAGTGG GTCAGACCTT TTTGTGCTAC CTGCTGGCCC GCTCGTGCCG CCTTCAGTTG 
GTCCGCCTCA CTGGCTATGG AAGGGGCGAG GTGCAGCTCT CCACGCACGC CTCCACGCTG 
GCCGCGAAAG ATGCAGTGGG TCTGAAGAGA ATGCACATGA TTGCCGTTCT CGATCCGAGT 
GGCAGCTTAC TTCTATACAC AGGCACCGTG CTCATATCCA AGGTGCACAT TACGCCGTTT 
TTGGCGCCTA CCTCCATTCC CACACCGCTG GTAACGCCCA TGACTGCTGC CCCATCTCCG 
TCGGCTTCGC CCGCTCCGTC TCACGTTAAA ACACCAATGG CAGCTGCTGG ACCAGCAAGC 
GGCATTCCGT CCGGCAGCAG TTCTTTCGTG GAGGTGCGAA GGAGCAGCCT ACTGCCCACA 
AAAGCGCCGG GGGATGTGGC TGCCTTCGAA GAGGAGCTGC ACATGCTGTC GCCCATCCAA 
CCGCAGCCGG TGTCCTACAC ACAGCGGCAG GCGCACAATG TCTGCAAATC CCTGAGAGAT 
CCGGCGGGAA ATCGATTGAC TTTGGTTTAT GCCACGGGCA GAATGCTCAG AATAGCGCTG 
CCATTTCTAA ATGATACACG ATTGCTCACC AGATGTGTGG CTACGCTACG GCAGGTTCTA 
AGTCCGACCC AGTTCCTGGA CTTTGTTATC CGTTGGTATA GCGACAGGAA TCCGCCGGGA 
TCGAGGAATT ACTCCATAGA GCAGGAATGG CTGCTATTCC GCTCCACACT GCTCGCCCTA 
ATGGGTCTGA CTGCTGCACC GGATGTGGAT GCGGGCGAGA ACTACGCTAG ATGTGCCACG 
CCACCGCTGC ACACTCAATT TGGAGGCGGC GCTACTGCGA CGGAATCGAG TGACGGTTCT 
AGTTGCAGTT CCAATTCTAC ACTGGGTGGC CAGGATGAGC CGAAGAAACG GCGAATATAC 
AATGATTGCG ATGATTTCAC GGACGACGAC TGGGAGTTCC TGCTGCTCCA GACGACACTG 
GCTCCCTGCG GAGCCGATGG CCACAGCTAC AGCGTGAACA TCGGTGCGCT GCTCTTCCGA 
ATGATTCCCG CCATCTTCTT CAGCCTGCAT TTACTGTACG AGGATCTCAA ACTGGACGCT 
GACTTTTATG GAGCGCTTCC ATATCTGGCA ACGTTCCTAC ACCAGCTGGC CATCGATATG 
CAGCTGGAAA GTTATGTGCT GCACTACATA CTAGACTTTC CGGAACTCTC GAATCGTACG 
GGCAAGCTCT CGTTGCTGGG AGCTGAGCAC GGAGCTATGA TGCTGCACCA GGAGCTTCTG 
AGGGTTCCGG CACCCAGTGT GTTTGCCCAA CTCGAGCACA TTATTGTCGG CGAGGAGGAG 
GTGATGCCTT ACACCTTCTT GGAGTGCGTT AACGAGAGAA GTCGCATTTT ACTGCAGCTC 
GTATCGCTGG TGACTCATGG GCATGAGCGT CTGAATTACT GGTGGCAGCT GCTTGAGATC 
CCCGGCGCCG TTCAGGCCAA CTTTACGCGG CGCTCTAAGC GTAATATCAC GGCGGATGCT 
CCGCGTAGCC ATCAGATGTT ACAGCTACTT CTGGCCATGC GCTTGACACG GCGAGACATT 
GAAAGATTCC CGGCGGCAGT GCATCTGATT GTAGCGGAGG CACTGGAGGA GGCTCGCCTC 
TCGCCGCCCA TGGGCTGCAG CATGGCCACC TATGAACTTA TCCTGCGTCC AGAATTGGCG 
GCCCACGCTC AACTGCCGTT CCTCGAAACG AGCACTGGGC AACCGCATTG TGGCCGGGTC 
TACAAGGAAG ATTCACTGTC AGCGAGGTGT CCGCCGACCG GGGGATCAGA AACTGATTCA 
CCGGCCCAAT TGCGCCGCGA CGACATGGAC AACATGGACA CAAAACTACT GCGTCTGCGT 
TTTCCCGATG ACATGCGGGT GGATGAAGTG CGCCGTCTGC TTAACAGCTC GGAACCGGTG 
GTCATTGAAG TGCAACAGGC ACCGGGAACC AGCGATCACG AATTCATTGA GGAGCAGGAG 
AAGCAACTGT TTGCACTATG TTCGCGCACA ATGACACTGC CCGTGGGCAG AGGTATGTTC 
ACCCTGAGAA CGATGTTGCC GCGGCCCAGC GAAAGCTTGA CCATGCCCAA ATTGTGCCTG 
TTGGGCAAGG AGCCGCTGAA GGGCACCACC ATCGAAATGC AGCAGATCGA ATTCCCAGCG 
AATATGCAAA TGTGGCCATC GTTTCACAAT GGCGTGGCCA CTGGCCTGAA GATCTCACCC 
CAGGCTCAGG ACATAGACTC CAATTGGATT GTCTACAACA AGCCCAAAAC GCATTCGCAT 
AATGCCCTGG AGCACGCTGG TTTCCTCATG GCCCTGGGCT TGAATGGTCA CCTAAAGACG 
CTGTCCTTCA TGAGTGTCTA TAAGTATTTG GTTAAATGCG ATGAGATGAC CAACGTTGGT 
CTGCTGCTGG GCATTTCCGC TGCCCATCGC GGCACCATGG ATACGAAAAC CACAAAGTTG 
TTGAGCGTTC ACTTGGAGGC ACTGCTGCCA GCCACCGCCA TGGAGTTGGA TATACCGCAA 
AGCACACAGG TGGCTGCTAT AATGGGCGTC GGTTTGCTCT ATCAAGGATC AGCTAAGCGC 
CACATAGCCG AGGTGCTGCT CCAGGAGATT GGCCGTCCTC CAGGCCCTGA GATGGAGAAC 
AGCATTGAGC GCGAATCGTA TGCCATGACA GCAGGTCTAT CCCTCGGATT GGTTACCCTC 
GGTCAGGGTG AATCGCCGGC GGGCCTAAGG GACCTCCAAC TGCCGGATAC CCTGCACTAC 
TACATGGTCG GTGGAGTGAA GAGACCCATC GGCGGTTCCC AGAAGGAGAA ATACCGACTA 
GCCTCGTTCC AAGTTCGTGA AGGCGATACC GTCAACATAG ATGTCACTGC TCCGGGTGCA 
ACGCTCGCAT TGGGACTTAT GTTCTTCAAC TCGGGGAACG CAGCTATTGC CGAATGGATG 
CAGCCGCCGG ATTCGCGGTA TCTACTGGAC ATGGTGCGGC CTGACTTCCT TCTGCTGCGC 
ACAATTTCCC GGGGCCTCAT CCTATGGCAG GATGTCCGGC CGGACAACGC GTGGTTCCAG 
GCACAGTTTC CGCGAGCACT CCGAGCTCAC CTAAAACTCC CGTTCTACGA AAATGAGTAT 
GCACCCGAGG ACTATGACGT GGACTACGAG GCCATCTCGC AAGCCTATTG CAATATCATG 
GCTGGAGCTG CCTTCTGCAT TGGTCTCAAG TACGCCGGCA CTGAGAATCT GGTGGCCTTT 
GCCACGCTGC GGTCGGTGAT AAAGGATTTC CTTCGCTTCC CCAGCAGGCC GATGGGCGAA 
TGTGCCGGTC GCACCACTGT GGAGAGCTGC CTGATGGTGC TGCTCATCTC GATCTCGTTG 
GTGTTCGCCG GCTCCGGCAA CTGCGAAATT CTTCGCATAA TTCGGTTTCT CAGGTCGCGA 
GTGGGACCAC AATATCCGCA CATAACCTAC GGCTCTCACA TGGCCATACA CATGTCGCTT 
GGCCTGCTAT TCCTCGGCGC GGGTCGCTTC ACCATTTCCC AGACACCGGA GTCGATTGCT 
GCACTGGTCT GCGCCTTCTT CCCCAAGTTT CCCATACACA GCAACGACAA TCGATACCAT 
CTGCAAGCGC TGAGACATCT CTATGTACTG GCTGTGGAAC CGCGCCTGTT CCTTCCCCGG 
GACATTGACA CCAATAAGCT GTGCCTGGCC AACATATCCG TGTTGGAGGT GGGTGCCACC 
GAGCTGCGCC GCCTGCCCAT CGCTCCCTGC ATCCTGCCAG TGCTGAGCAC CCTGCAGCAG 
GTGGTGGTGG ACGACGAGAA CTATTGGCCA GTGTGCTTCG AGCGATCGCG CAACTGGGAT 
CAGCTGGAAA AGGCGCTGGA GATGAGTGCA CCGATTGATA TTAAGAAGCG AACGGGCTGC 
CTTTCCCACT TGGAGGACCC GGACAGGCTG AAGAGCATGC TGGCCCAAAC ATTGACGATG 
GAACAGAGCA TCTGTTGGCA GATCGATATG AACGACCTGC AGCAGTTTGC CAGCGAACGT 
ATGGTGAAGC AGTTCCTCAG TCGCTGCCTG GACACCAAAG GCACGGATTT GAGTCCCCCG 
GAGCTGATGA AGCGGCACCA GGTGATGCTG CTATTCTACA ATGCCGTGGT CAAGGATCGC 
ATGCATTTCC TGCCGGTTTA CCTAACCCTA TACGATCACG TAACCAAGTC CATGCCGAAC 
AACATCGATG TGTGGCAGAT GAAGCTCATC GATGCCTATT TGAGTCGCAG CCAGGAGTCC 
GAGCATCCGC TCATCTCCGT GGAGCTTATC CAAATGATGC AAGAGCTCTT CAAGCAGGAA 
ATGGAGGACT CGACGCGCGA GTTGTGCTTA CCGCTGCGCG AGTTCCTCAG CCGAAGGCGT 
CTGGATCCCA GCTATGTGAC CACAGTCAGT GGACCGGACT TGCAGCGAGC CTTCTGCGTG 
ATCAATTACT ACAATCTGTT GCCGAATATG TTGAATGGAG TGGACTTGAG CACGGGCACA 
GTTAACTATA TGCGTCTTTT ATACGAATTC CGCCGGCTGA ATCTCGGCGC GCACACCATA 
TTTGGCCTAA TGAAGATCCT ACAGTCACTG GCCACCGAAG TGGTGACTCT GGATGAGGCA 
GCCCTTCTGG CGTACACCAT GGGTGACCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_573025.2
CDS	107..6199
Translation	MIAVAEPPLEFVPRGRQTSAEHPGPHDQPMARHQLPTTEHLLLQRLQNVNISSAGEDCPSGGQESWTVRELYDDYEASEERAALRRKLTERTKRCGLQATSLPNRLLNPPLVRRVEPMCDYMVNNEEELYVNRNTVVWTQGVNDDDDTDNGVYRRMCFTTDTPVRFACFLNRSFVRGRLAQLKAAVHPDDDHLTAICVMDQDALRVYCSNGEDFLANLDFPVSQLWQTKYGLLLEKDSSNALISHMSIPMPRLFSMSHPLHEACPVVLKTATGSTGYMTEPEYTVVFTTEESDLVMLYDAKFFKHFVARLRKVTPEEINYVSQQMELGQTLMGPRSMAGNSFSSTKQTGATPKATNLSFAARNINTTTTGMGNQFGLSQSQSFSGVLGQSNRASLGTPLSQLQSSISQQSMSVKDMRKLTHVKPAKPIEPELCMEHIWTENIYGTQREFCEMATRAFIHTDLVGQTFLCYLLARSCRLQLVRLTGYGRGEVQLSTHASTLAAKDAVGLKRMHMIAVLDPSGSLLLYTGTVLISKVHITPFLAPTSIPTPLVTPMTAAPSPSASPAPSHVKTPMAAAGPASGIPSGSSSFVEVRRSSLLPTKAPGDVAAFEEELHMLSPIQPQPVSYTQRQAHNVCKSLRDPAGNRLTLVYATGRMLRIALPFLNDTRLLTRCVATLRQVLSPTQFLDFVIRWYSDRNPPGSRNYSIEQEWLLFRSTLLALMGLTAAPDVDAGENYARCATPPLHTQFGGGATATESSDGSSCSSNSTLGGQDEPKKRRIYNDCDDFTDDDWEFLLLQTTLAPCGADGHSYSVNIGALLFRMIPAIFFSLHLLYEDLKLDADFYGALPYLATFLHQLAIDMQLESYVLHYILDFPELSNRTGKLSLLGAEHGAMMLHQELLRVPAPSVFAQLEHIIVGEEEVMPYTFLECVNERSRILLQLVSLVTHGHERLNYWWQLLEIPGAVQANFTRRSKRNITADAPRSHQMLQLLLAMRLTRRDIERFPAAVHLIVAEALEEARLSPPMGCSMATYELILRPELAAHAQLPFLETSTGQPHCGRVYKEDSLSARCPPTGGSETDSPAQLRRDDMDNMDTKLLRLRFPDDMRVDEVRRLLNSSEPVVIEVQQAPGTSDHEFIEEQEKQLFALCSRTMTLPVGRGMFTLRTMLPRPSESLTMPKLCLLGKEPLKGTTIEMQQIEFPANMQMWPSFHNGVATGLKISPQAQDIDSNWIVYNKPKTHSHNALEHAGFLMALGLNGHLKTLSFMSVYKYLVKCDEMTNVGLLLGISAAHRGTMDTKTTKLLSVHLEALLPATAMELDIPQSTQVAAIMGVGLLYQGSAKRHIAEVLLQEIGRPPGPEMENSIERESYAMTAGLSLGLVTLGQGESPAGLRDLQLPDTLHYYMVGGVKRPIGGSQKEKYRLASFQVREGDTVNIDVTAPGATLALGLMFFNSGNAAIAEWMQPPDSRYLLDMVRPDFLLLRTISRGLILWQDVRPDNAWFQAQFPRALRAHLKLPFYENEYAPEDYDVDYEAISQAYCNIMAGAAFCIGLKYAGTENLVAFATLRSVIKDFLRFPSRPMGECAGRTTVESCLMVLLISISLVFAGSGNCEILRIIRFLRSRVGPQYPHITYGSHMAIHMSLGLLFLGAGRFTISQTPESIAALVCAFFPKFPIHSNDNRYHLQALRHLYVLAVEPRLFLPRDIDTNKLCLANISVLEVGATELRRLPIAPCILPVLSTLQQVVVDDENYWPVCFERSRNWDQLEKALEMSAPIDIKKRTGCLSHLEDPDRLKSMLAQTLTMEQSICWQIDMNDLQQFASERMVKQFLSRCLDTKGTDLSPPELMKRHQVMLLFYNAVVKDRMHFLPVYLTLYDHVTKSMPNNIDVWQMKLIDAYLSRSQESEHPLISVELIQMMQELFKQEMEDSTRELCLPLREFLSRRRLDPSYVTTVSGPDLQRAFCVINYYNLLPNMLNGVDLSTGTVNYMRLLYEFRRLNLGAHTIFGLMKILQSLATEVVTLDEAALLAYTMGDQ

Target ORF information:

RefSeq Version	NM_132797.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster shattered (shtd), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132797.3

ORF Insert Sequence:

ATGATCGCCG TCGCAGAACC GCCGCTGGAG TTTGTGCCGC GCGGCCGTCA GACGTCCGCG 
GAGCATCCGG GCCCACATGA CCAGCCAATG GCGCGCCACC AACTGCCCAC CACGGAGCAT 
CTGCTGCTGC AGCGCCTCCA GAATGTGAAC ATCTCGTCGG CCGGTGAGGA CTGCCCTTCG 
GGCGGACAGG AGTCGTGGAC AGTCCGAGAG CTGTACGATG ACTACGAGGC ATCCGAGGAG 
CGCGCCGCCC TGCGACGCAA GTTGACCGAG CGGACAAAGC GATGTGGTCT CCAGGCCACC 
TCGCTGCCGA ACAGACTGCT CAATCCACCG CTGGTGCGGC GCGTGGAGCC AATGTGCGAC 
TATATGGTTA ACAACGAGGA GGAGCTCTAT GTGAACCGGA ACACGGTGGT GTGGACACAA 
GGCGTCAACG ATGACGACGA TACCGACAAT GGGGTGTACC GGCGCATGTG TTTCACCACC 
GATACGCCGG TGCGCTTCGC CTGCTTCCTG AATCGCAGTT TTGTGCGCGG GCGACTGGCG 
CAGCTGAAGG CGGCTGTCCA CCCGGATGAC GACCATTTGA CCGCCATCTG TGTGATGGAT 
CAGGACGCCC TGCGGGTGTA CTGCAGCAAC GGCGAGGACT TTCTAGCCAA TCTGGACTTT 
CCCGTGTCAC AGCTGTGGCA AACCAAGTAT GGCCTGCTGC TGGAAAAGGA CTCCAGCAAT 
GCCTTGATAT CGCACATGTC CATTCCGATG CCGCGTCTCT TCTCTATGTC GCATCCGCTG 
CACGAGGCCT GTCCCGTCGT CCTGAAGACG GCCACCGGCT CCACCGGCTA CATGACCGAA 
CCGGAATACA CTGTGGTCTT CACCACCGAG GAATCGGATT TGGTGATGCT GTACGATGCC 
AAGTTCTTTA AGCATTTCGT TGCACGCCTG CGTAAGGTAA CGCCCGAAGA GATCAACTAT 
GTGAGTCAGC AGATGGAGCT GGGACAGACG CTAATGGGAC CCAGGTCGAT GGCAGGCAAT 
AGTTTCAGTT CCACCAAGCA AACTGGAGCC ACGCCAAAAG CAACGAACTT ATCGTTTGCG 
GCCAGGAATA TCAACACCAC CACCACCGGC ATGGGCAACC AGTTCGGACT CAGCCAGTCG 
CAGTCGTTCA GCGGAGTTCT TGGACAGTCG AATCGCGCCT CCCTAGGCAC TCCATTGAGC 
CAACTGCAGA GCAGTATTAG CCAGCAGTCC ATGTCCGTGA AGGATATGCG CAAGTTAACG 
CACGTGAAGC CGGCAAAACC CATTGAGCCG GAATTGTGTA TGGAGCACAT CTGGACGGAG 
AACATCTACG GAACTCAACG AGAGTTCTGC GAGATGGCCA CCCGAGCATT TATCCACACG 
GATTTAGTGG GTCAGACCTT TTTGTGCTAC CTGCTGGCCC GCTCGTGCCG CCTTCAGTTG 
GTCCGCCTCA CTGGCTATGG AAGGGGCGAG GTGCAGCTCT CCACGCACGC CTCCACGCTG 
GCCGCGAAAG ATGCAGTGGG TCTGAAGAGA ATGCACATGA TTGCCGTTCT CGATCCGAGT 
GGCAGCTTAC TTCTATACAC AGGCACCGTG CTCATATCCA AGGTGCACAT TACGCCGTTT 
TTGGCGCCTA CCTCCATTCC CACACCGCTG GTAACGCCCA TGACTGCTGC CCCATCTCCG 
TCGGCTTCGC CCGCTCCGTC TCACGTTAAA ACACCAATGG CAGCTGCTGG ACCAGCAAGC 
GGCATTCCGT CCGGCAGCAG TTCTTTCGTG GAGGTGCGAA GGAGCAGCCT ACTGCCCACA 
AAAGCGCCGG GGGATGTGGC TGCCTTCGAA GAGGAGCTGC ACATGCTGTC GCCCATCCAA 
CCGCAGCCGG TGTCCTACAC ACAGCGGCAG GCGCACAATG TCTGCAAATC CCTGAGAGAT 
CCGGCGGGAA ATCGATTGAC TTTGGTTTAT GCCACGGGCA GAATGCTCAG AATAGCGCTG 
CCATTTCTAA ATGATACACG ATTGCTCACC AGATGTGTGG CTACGCTACG GCAGGTTCTA 
AGTCCGACCC AGTTCCTGGA CTTTGTTATC CGTTGGTATA GCGACAGGAA TCCGCCGGGA 
TCGAGGAATT ACTCCATAGA GCAGGAATGG CTGCTATTCC GCTCCACACT GCTCGCCCTA 
ATGGGTCTGA CTGCTGCACC GGATGTGGAT GCGGGCGAGA ACTACGCTAG ATGTGCCACG 
CCACCGCTGC ACACTCAATT TGGAGGCGGC GCTACTGCGA CGGAATCGAG TGACGGTTCT 
AGTTGCAGTT CCAATTCTAC ACTGGGTGGC CAGGATGAGC CGAAGAAACG GCGAATATAC 
AATGATTGCG ATGATTTCAC GGACGACGAC TGGGAGTTCC TGCTGCTCCA GACGACACTG 
GCTCCCTGCG GAGCCGATGG CCACAGCTAC AGCGTGAACA TCGGTGCGCT GCTCTTCCGA 
ATGATTCCCG CCATCTTCTT CAGCCTGCAT TTACTGTACG AGGATCTCAA ACTGGACGCT 
GACTTTTATG GAGCGCTTCC ATATCTGGCA ACGTTCCTAC ACCAGCTGGC CATCGATATG 
CAGCTGGAAA GTTATGTGCT GCACTACATA CTAGACTTTC CGGAACTCTC GAATCGTACG 
GGCAAGCTCT CGTTGCTGGG AGCTGAGCAC GGAGCTATGA TGCTGCACCA GGAGCTTCTG 
AGGGTTCCGG CACCCAGTGT GTTTGCCCAA CTCGAGCACA TTATTGTCGG CGAGGAGGAG 
GTGATGCCTT ACACCTTCTT GGAGTGCGTT AACGAGAGAA GTCGCATTTT ACTGCAGCTC 
GTATCGCTGG TGACTCATGG GCATGAGCGT CTGAATTACT GGTGGCAGCT GCTTGAGATC 
CCCGGCGCCG TTCAGGCCAA CTTTACGCGG CGCTCTAAGC GTAATATCAC GGCGGATGCT 
CCGCGTAGCC ATCAGATGTT ACAGCTACTT CTGGCCATGC GCTTGACACG GCGAGACATT 
GAAAGATTCC CGGCGGCAGT GCATCTGATT GTAGCGGAGG CACTGGAGGA GGCTCGCCTC 
TCGCCGCCCA TGGGCTGCAG CATGGCCACC TATGAACTTA TCCTGCGTCC AGAATTGGCG 
GCCCACGCTC AACTGCCGTT CCTCGAAACG AGCACTGGGC AACCGCATTG TGGCCGGGTC 
TACAAGGAAG ATTCACTGTC AGCGAGGTGT CCGCCGACCG GGGGATCAGA AACTGATTCA 
CCGGCCCAAT TGCGCCGCGA CGACATGGAC AACATGGACA CAAAACTACT GCGTCTGCGT 
TTTCCCGATG ACATGCGGGT GGATGAAGTG CGCCGTCTGC TTAACAGCTC GGAACCGGTG 
GTCATTGAAG TGCAACAGGC ACCGGGAACC AGCGATCACG AATTCATTGA GGAGCAGGAG 
AAGCAACTGT TTGCACTATG TTCGCGCACA ATGACACTGC CCGTGGGCAG AGGTATGTTC 
ACCCTGAGAA CGATGTTGCC GCGGCCCAGC GAAAGCTTGA CCATGCCCAA ATTGTGCCTG 
TTGGGCAAGG AGCCGCTGAA GGGCACCACC ATCGAAATGC AGCAGATCGA ATTCCCAGCG 
AATATGCAAA TGTGGCCATC GTTTCACAAT GGCGTGGCCA CTGGCCTGAA GATCTCACCC 
CAGGCTCAGG ACATAGACTC CAATTGGATT GTCTACAACA AGCCCAAAAC GCATTCGCAT 
AATGCCCTGG AGCACGCTGG TTTCCTCATG GCCCTGGGCT TGAATGGTCA CCTAAAGACG 
CTGTCCTTCA TGAGTGTCTA TAAGTATTTG GTTAAATGCG ATGAGATGAC CAACGTTGGT 
CTGCTGCTGG GCATTTCCGC TGCCCATCGC GGCACCATGG ATACGAAAAC CACAAAGTTG 
TTGAGCGTTC ACTTGGAGGC ACTGCTGCCA GCCACCGCCA TGGAGTTGGA TATACCGCAA 
AGCACACAGG TGGCTGCTAT AATGGGCGTC GGTTTGCTCT ATCAAGGATC AGCTAAGCGC 
CACATAGCCG AGGTGCTGCT CCAGGAGATT GGCCGTCCTC CAGGCCCTGA GATGGAGAAC 
AGCATTGAGC GCGAATCGTA TGCCATGACA GCAGGTCTAT CCCTCGGATT GGTTACCCTC 
GGTCAGGGTG AATCGCCGGC GGGCCTAAGG GACCTCCAAC TGCCGGATAC CCTGCACTAC 
TACATGGTCG GTGGAGTGAA GAGACCCATC GGCGGTTCCC AGAAGGAGAA ATACCGACTA 
GCCTCGTTCC AAGTTCGTGA AGGCGATACC GTCAACATAG ATGTCACTGC TCCGGGTGCA 
ACGCTCGCAT TGGGACTTAT GTTCTTCAAC TCGGGGAACG CAGCTATTGC CGAATGGATG 
CAGCCGCCGG ATTCGCGGTA TCTACTGGAC ATGGTGCGGC CTGACTTCCT TCTGCTGCGC 
ACAATTTCCC GGGGCCTCAT CCTATGGCAG GATGTCCGGC CGGACAACGC GTGGTTCCAG 
GCACAGTTTC CGCGAGCACT CCGAGCTCAC CTAAAACTCC CGTTCTACGA AAATGAGTAT 
GCACCCGAGG ACTATGACGT GGACTACGAG GCCATCTCGC AAGCCTATTG CAATATCATG 
GCTGGAGCTG CCTTCTGCAT TGGTCTCAAG TACGCCGGCA CTGAGAATCT GGTGGCCTTT 
GCCACGCTGC GGTCGGTGAT AAAGGATTTC CTTCGCTTCC CCAGCAGGCC GATGGGCGAA 
TGTGCCGGTC GCACCACTGT GGAGAGCTGC CTGATGGTGC TGCTCATCTC GATCTCGTTG 
GTGTTCGCCG GCTCCGGCAA CTGCGAAATT CTTCGCATAA TTCGGTTTCT CAGGTCGCGA 
GTGGGACCAC AATATCCGCA CATAACCTAC GGCTCTCACA TGGCCATACA CATGTCGCTT 
GGCCTGCTAT TCCTCGGCGC GGGTCGCTTC ACCATTTCCC AGACACCGGA GTCGATTGCT 
GCACTGGTCT GCGCCTTCTT CCCCAAGTTT CCCATACACA GCAACGACAA TCGATACCAT 
CTGCAAGCGC TGAGACATCT CTATGTACTG GCTGTGGAAC CGCGCCTGTT CCTTCCCCGG 
GACATTGACA CCAATAAGCT GTGCCTGGCC AACATATCCG TGTTGGAGGT GGGTGCCACC 
GAGCTGCGCC GCCTGCCCAT CGCTCCCTGC ATCCTGCCAG TGCTGAGCAC CCTGCAGCAG 
GTGGTGGTGG ACGACGAGAA CTATTGGCCA GTGTGCTTCG AGCGATCGCG CAACTGGGAT 
CAGCTGGAAA AGGCGCTGGA GATGAGTGCA CCGATTGATA TTAAGAAGCG AACGGGCTGC 
CTTTCCCACT TGGAGGACCC GGACAGGCTG AAGAGCATGC TGGCCCAAAC ATTGACGATG 
GAACAGAGCA TCTGTTGGCA GATCGATATG AACGACCTGC AGCAGTTTGC CAGCGAACGT 
ATGGTGAAGC AGTTCCTCAG TCGCTGCCTG GACACCAAAG GCACGGATTT GAGTCCCCCG 
GAGCTGATGA AGCGGCACCA GGTGATGCTG CTATTCTACA ATGCCGTGGT CAAGGATCGC 
ATGCATTTCC TGCCGGTTTA CCTAACCCTA TACGATCACG TAACCAAGTC CATGCCGAAC 
AACATCGATG TGTGGCAGAT GAAGCTCATC GATGCCTATT TGAGTCGCAG CCAGGAGTCC 
GAGCATCCGC TCATCTCCGT GGAGCTTATC CAAATGATGC AAGAGCTCTT CAAGCAGGAA 
ATGGAGGACT CGACGCGCGA GTTGTGCTTA CCGCTGCGCG AGTTCCTCAG CCGAAGGCGT 
CTGGATCCCA GCTATGTGAC CACAGTCAGT GGACCGGACT TGCAGCGAGC CTTCTGCGTG 
ATCAATTACT ACAATCTGTT GCCGAATATG TTGAATGGAG TGGACTTGAG CACGGGCACA 
GTTAACTATA TGCGTCTTTT ATACGAATTC CGCCGGCTGA ATCTCGGCGC GCACACCATA 
TTTGGCCTAA TGAAGATCCT ACAGTCACTG GCCACCGAAG TGGTGACTCT GGATGAGGCA 
GCCCTTCTGG CGTACACCAT GGGTGACCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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shtd cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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