Top1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Top1
Entrez Gene ID	32458
Full Name	Topoisomerase 1
Synonyms	CG6146,Dmel\CG6146,TOP1,TopI,Topo I,TopoI,dTopoI,l(1)G0134,l(1)G0201,l(1)G0229,l(1)G0278,l(1)top1,top1,topo I,topoI
General protein information	Preferred Names Topoisomerase 1 Names CG6146 gene product from transcript CG6146-RA CG6146-PA CG6146-PB CG6146-PC CG6146-PD CG6146-PE Top 1 Top1-PA Top1-PB Top1-PC Top1-PD Top1-PE topoisomerase 1 topoisomerase I topoisomerase1 type I topoisomerase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 13B6-13B6

mRNA and Protein(s)

mRNA	Protein	Name
NM_078606.4	NP_511161.2	topoisomerase 1, isoform A
NM_001014742.2	NP_001014742.1	topoisomerase 1, isoform C
NM_001298338.1	NP_001285267.1	topoisomerase 1, isoform E
NM_001258750.2	NP_001245679.1	topoisomerase 1, isoform D
NM_167435.3	NP_727841.1	topoisomerase 1, isoform B

Pathways from BioSystems

Homologies

	TOP1	XP_001088021.1
Bos taurus (cattle)	TOP1	NP_001193416.1
Gallus gallus (chicken)	TOP1	NP_990441.1
Neurospora crassa	NCU09118	XP_958033.2
Arabidopsis thaliana (thale cress)	TOP1ALPHA	NP_200341.1
Canis lupus familiaris (dog)	TOP1	XP_534420.3
Caenorhabditis elegans (roundworm)	top-1	NP_001021578.1
Saccharomyces cerevisiae (baker's yeast)	TOP1	NP_014637.1
Magnaporthe oryzae (rice blast fungus)	MGG_09917	XP_003710015.1
Arabidopsis thaliana (thale cress)	TOP1BETA	NP_200342.1
Kluyveromyces lactis	KLLA0C06116g	XP_452470.1
Eremothecium gossypii	AGOS_ACL008C	NP_983396.2
Xenopus tropicalis (tropical clawed frog)	LOC100496521	XP_002932918.2
Mus musculus (house mouse)	Top1	NP_033434.2
Danio rerio (zebrafish)	top1l	NP_001037789.1
Oryza sativa (rice)	Os08g0154600	NP_001061015.1
Homo sapiens (human)	TOP1	NP_003277.1
Pan troglodytes (chimpanzee)	TOP1	XP_514649.2
Rattus norvegicus (Norway rat)	Top1	NP_072137.1
Drosophila melanogaster (fruit fly)	Top1	NP_001014742.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004078	XP_003436707.1
Schizosaccharomyces pombe (fission yeast)	top1	NP_596209.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

B52 delivers Topo I to RNA polymerase II-active chromatin loci and DNA topology and mRNA release can be coordinated to control gene expression.
Title: The SR protein B52/SRp55 is required for DNA topoisomerase I recruitment to chromatin, mRNA release and transcription shutdown.

In a model of human epilepsy, topo I shows a novel function in construction and/or maintenance of circuits required for seizure propagation in vivo.
Title: Seizure suppression by top1 mutations in Drosophila.

The following Top1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Top1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01668	NM_167435.3 Latest version!	Drosophila melanogaster topoisomerase 1, transcript variant B (Top1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa21479	NM_001298338.1 Latest version!	Drosophila melanogaster topoisomerase 1, transcript variant E (Top1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa01667	NM_078606.4 Latest version!	Drosophila melanogaster topoisomerase 1, transcript variant A (Top1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa01667	NM_001014742.2 Latest version!	Drosophila melanogaster topoisomerase 1, transcript variant C (Top1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa02721	NM_001258750.2 Latest version!	Drosophila melanogaster topoisomerase 1, transcript variant D (Top1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Top1 ( NM_167435.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727841.1 Drosophila melanogaster topoisomerase 1, transcript variant B (Top1), mRNA.

Price & Availability↑

CloneID	OFa01668
Clone ID Related Accession (Same CDS sequence)	NM_167435.3
Accession Version	NM_167435.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	topoisomerase 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167435.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCTTATA ATCGAAAAAA TTTTTTTTGT CAGGAGCCCG AGCCTGCCGT ATCCCCCGGC 
AAACGACAGA AGGCAAAGGC CAAGGTGGAG GAGGAGGAGG TTTGGCGATG GTGGGAGGAG 
GAGAAGCGTG CCGATGGCGT CAAATGGTCA ACGCTGGAGC ACAAGGGACC CGTGTTTGCA 
CCGCGATACG AACGGGTGCC ACGCAATGTA CGGTTCTACT ACGATGGCAA GCCGTTGGAG 
CTCTCTGAGG AAACGGAGGA GGCAGCCACG TTCTATGCCA AGATGCTGAA CCACGACTAC 
TGCACCAAGG AAGTGTTCAA TAACAACTTC TTCAAGGACT TCCGCAAGTC CATGACGCCC 
AGAGAGAGGG AGATTATCAA GGATTTCCGC AAGTGCAACT TCCAGGAGAT GTTCAACTAC 
TTCCAAGCGG AGTCCGAAAA GCGCAAGGCA GCCAGCAAGG AGGAGAAGCT GATCAAGAAG 
AACGAAAACG AGGCGCTGAT GAAGGAATTC GGATTCTGCA TGATTGATGG GCACAAGGAG 
AAGATCGGTA ACTTCCGCCT GGAGCCGCCA GGCCTGTTCC GCGGTCGTGG CGAGCATCCC 
AAAATGGGCA TGATCAAGCG GCGCATCCAA GCCAGCGATG TGTCCATTAA CTGTGGCAAA 
GATTCAAAGG TGCCTTCACC GCCGCCCGGA TCTCGCTGGA AGGAGGTGCG CCACGACAAC 
ACGGTCACCT GGCTGGCCTC CTGGATCGAG AATGTGCAGG GACAGGTCAA GTACATCATG 
TTGAATCCCT CCTCGAAACT CAAGGGCGAG AAGGATCACA TTAAGTACGA GACGGCGCGA 
CGCCTGGACA AGGTAATCGA TAAGATACGT GCCACCTATC GCGACGAGTG GAAGTCCAAG 
GAGATGAGGG TTCGCCAGCG AGCGGTGGCC CTTTACTTCA TCGACAAACT GGCGCTGAGA 
GCAGGCAACG AAAAGGACGA GGATCAGGCC GATACCGTGG GCTGTTGCTC GCTACGCGTG 
GAGCATGTCC AGCTCCACAA GGAACTGAAC GGAAAGGAGA ACGTGGTGGT CTTTGATTTC 
CCCGGTAAGG ACTCCATTCG GTATTACAAC GAGGTTGAGG TGGAGAAGCG CGTCTTTAAG 
AACCTCGAAC TGTTCATGGA GCACAAGAAG GAGGGCGACG ACCTCTTCGA TCGTCTCAAC 
ACGCAGGTGC TTAATGAGCA TCTCAAGGAG CTGATGGAAG GTCTCACGGC CAAGGTATTC 
CGTACGTACA ACGCATCGAA AACACTGCAA AGTCAGCTGG ATCTGTTGAC CGATCCGAGC 
GCCACGGTAC CGGAAAAGCT GTTGGCCTAC AATCGCGCCA ACCGTGCCGT GGCCATCCTC 
TGTAACCATC AGCGTTCCGT GCCCAAGAGC CACGAGAAGT CAATGGAGAA TCTGAAGGAG 
AAGATCAAGG CCAAGCGGGA AGCCATCGAA AAATGCGAGT CCGAGTATCA TTCGAGAGAC 
GAGAAGAAGG GCAAACAGCT GGAGCGCCTA AGGGATCAGC TGAAGAAACT AGAACTACAA 
GAGACTGATA GAGACGAGAA TAAGACCATT GCCCTGGGCA CATCTAAGCT AAACTATCTT 
GATCCACGCA TATCGGTGGC TTGGTGTAAA AAGCATGACG TGCCGATTGA GAAGATATTC 
AACAAAACGC AAAGAACCAA ATTTCTGTGG GCCGTTCATA TGGCCGATGA GAATTATCGT 
TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727841.1
CDS	21..1766
Translation	MPYNRKNFFCQEPEPAVSPGKRQKAKAKVEEEEVWRWWEEEKRADGVKWSTLEHKGPVFAPRYERVPRNVRFYYDGKPLELSEETEEAATFYAKMLNHDYCTKEVFNNNFFKDFRKSMTPREREIIKDFRKCNFQEMFNYFQAESEKRKAASKEEKLIKKNENEALMKEFGFCMIDGHKEKIGNFRLEPPGLFRGRGEHPKMGMIKRRIQASDVSINCGKDSKVPSPPPGSRWKEVRHDNTVTWLASWIENVQGQVKYIMLNPSSKLKGEKDHIKYETARRLDKVIDKIRATYRDEWKSKEMRVRQRAVALYFIDKLALRAGNEKDEDQADTVGCCSLRVEHVQLHKELNGKENVVVFDFPGKDSIRYYNEVEVEKRVFKNLELFMEHKKEGDDLFDRLNTQVLNEHLKELMEGLTAKVFRTYNASKTLQSQLDLLTDPSATVPEKLLAYNRANRAVAILCNHQRSVPKSHEKSMENLKEKIKAKREAIEKCESEYHSRDEKKGKQLERLRDQLKKLELQETDRDENKTIALGTSKLNYLDPRISVAWCKKHDVPIEKIFNKTQRTKFLWAVHMADENYRF

Target ORF information:

RefSeq Version	NM_167435.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster topoisomerase 1, transcript variant B (Top1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167435.3

ORF Insert Sequence:

ATGCCTTATA ATCGAAAAAA TTTTTTTTGT CAGGAGCCCG AGCCTGCCGT ATCCCCCGGC 
AAACGACAGA AGGCAAAGGC CAAGGTGGAG GAGGAGGAGG TTTGGCGATG GTGGGAGGAG 
GAGAAGCGTG CCGATGGCGT CAAATGGTCA ACGCTGGAGC ACAAGGGACC CGTGTTTGCA 
CCGCGATACG AACGGGTGCC ACGCAATGTA CGGTTCTACT ACGATGGCAA GCCGTTGGAG 
CTCTCTGAGG AAACGGAGGA GGCAGCCACG TTCTATGCCA AGATGCTGAA CCACGACTAC 
TGCACCAAGG AAGTGTTCAA TAACAACTTC TTCAAGGACT TCCGCAAGTC CATGACGCCC 
AGAGAGAGGG AGATTATCAA GGATTTCCGC AAGTGCAACT TCCAGGAGAT GTTCAACTAC 
TTCCAAGCGG AGTCCGAAAA GCGCAAGGCA GCCAGCAAGG AGGAGAAGCT GATCAAGAAG 
AACGAAAACG AGGCGCTGAT GAAGGAATTC GGATTCTGCA TGATTGATGG GCACAAGGAG 
AAGATCGGTA ACTTCCGCCT GGAGCCGCCA GGCCTGTTCC GCGGTCGTGG CGAGCATCCC 
AAAATGGGCA TGATCAAGCG GCGCATCCAA GCCAGCGATG TGTCCATTAA CTGTGGCAAA 
GATTCAAAGG TGCCTTCACC GCCGCCCGGA TCTCGCTGGA AGGAGGTGCG CCACGACAAC 
ACGGTCACCT GGCTGGCCTC CTGGATCGAG AATGTGCAGG GACAGGTCAA GTACATCATG 
TTGAATCCCT CCTCGAAACT CAAGGGCGAG AAGGATCACA TTAAGTACGA GACGGCGCGA 
CGCCTGGACA AGGTAATCGA TAAGATACGT GCCACCTATC GCGACGAGTG GAAGTCCAAG 
GAGATGAGGG TTCGCCAGCG AGCGGTGGCC CTTTACTTCA TCGACAAACT GGCGCTGAGA 
GCAGGCAACG AAAAGGACGA GGATCAGGCC GATACCGTGG GCTGTTGCTC GCTACGCGTG 
GAGCATGTCC AGCTCCACAA GGAACTGAAC GGAAAGGAGA ACGTGGTGGT CTTTGATTTC 
CCCGGTAAGG ACTCCATTCG GTATTACAAC GAGGTTGAGG TGGAGAAGCG CGTCTTTAAG 
AACCTCGAAC TGTTCATGGA GCACAAGAAG GAGGGCGACG ACCTCTTCGA TCGTCTCAAC 
ACGCAGGTGC TTAATGAGCA TCTCAAGGAG CTGATGGAAG GTCTCACGGC CAAGGTATTC 
CGTACGTACA ACGCATCGAA AACACTGCAA AGTCAGCTGG ATCTGTTGAC CGATCCGAGC 
GCCACGGTAC CGGAAAAGCT GTTGGCCTAC AATCGCGCCA ACCGTGCCGT GGCCATCCTC 
TGTAACCATC AGCGTTCCGT GCCCAAGAGC CACGAGAAGT CAATGGAGAA TCTGAAGGAG 
AAGATCAAGG CCAAGCGGGA AGCCATCGAA AAATGCGAGT CCGAGTATCA TTCGAGAGAC 
GAGAAGAAGG GCAAACAGCT GGAGCGCCTA AGGGATCAGC TGAAGAAACT AGAACTACAA 
GAGACTGATA GAGACGAGAA TAAGACCATT GCCCTGGGCA CATCTAAGCT AAACTATCTT 
GATCCACGCA TATCGGTGGC TTGGTGTAAA AAGCATGACG TGCCGATTGA GAAGATATTC 
AACAAAACGC AAAGAACCAA ATTTCTGTGG GCCGTTCATA TGGCCGATGA GAATTATCGT 
TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Top1 ( NM_001298338.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285267.1 Drosophila melanogaster topoisomerase 1, transcript variant E (Top1), mRNA.

Price & Availability↑

CloneID	OFa21479
Clone ID Related Accession (Same CDS sequence)	NM_001298338.1
Accession Version	NM_001298338.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	topoisomerase 1, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGCGCT CTTGGGTGGC ATTGCGTCCA TTCGAAAAGC AGAGTGTCTG CCTGGTGCTC 
GGACAGCGTA TGTTGGCCGT AGCCACCGTT GCGGGCAAAC GGAGAAGAGT GCGACGGAAG 
AGCGTCCAGG AAGAGCAAAT TCGATGGTGG GAGGAGGAGA AGCGTGCCGA TGGCGTCAAA 
TGGTCAACGC TGGAGCACAA GGGACCCGTG TTTGCACCGC GATACGAACG GGTGCCACGC 
AATGTACGGT TCTACTACGA TGGCAAGCCG TTGGAGCTCT CTGAGGAAAC GGAGGAGGCA 
GCCACGTTCT ATGCCAAGAT GCTGAACCAC GACTACTGCA CCAAGGAAGT GTTCAATAAC 
AACTTCTTCA AGGACTTCCG CAAGTCCATG ACGCCCAGAG AGAGGGAGAT TATCAAGGAT 
TTCCGCAAGT GCAACTTCCA GGAGATGTTC AACTACTTCC AAGCGGAGTC CGAAAAGCGC 
AAGGCAGCCA GCAAGGAGGA GAAGCTGATC AAGAAGAACG AAAACGAGGC GCTGATGAAG 
GAATTCGGAT TCTGCATGAT TGATGGGCAC AAGGAGAAGA TCGGTAACTT CCGCCTGGAG 
CCGCCAGGCC TGTTCCGCGG TCGTGGCGAG CATCCCAAAA TGGGCATGAT CAAGCGGCGC 
ATCCAAGCCA GCGATGTGTC CATTAACTGT GGCAAAGATT CAAAGGTGCC TTCACCGCCG 
CCCGGATCTC GCTGGAAGGA GGTGCGCCAC GACAACACGG TCACCTGGCT GGCCTCCTGG 
ATCGAGAATG TGCAGGGACA GGTCAAGTAC ATCATGTTGA ATCCCTCCTC GAAACTCAAG 
GGCGAGAAGG ATCACATTAA GTACGAGACG GCGCGACGCC TGGACAAGGT AATCGATAAG 
ATACGTGCCA CCTATCGCGA CGAGTGGAAG TCCAAGGAGA TGAGGGTTCG CCAGCGAGCG 
GTGGCCCTTT ACTTCATCGA CAAACTGGCG CTGAGAGCAG GCAACGAAAA GGACGAGGAT 
CAGGCCGATA CCGTGGGCTG TTGCTCGCTA CGCGTGGAGC ATGTCCAGCT CCACAAGGAA 
CTGAACGGAA AGGAGAACGT GGTGGTCTTT GATTTCCCCG GTAAGGACTC CATTCGGTAT 
TACAACGAGG TTGAGGTGGA GAAGCGCGTC TTTAAGAACC TCGAACTGTT CATGGAGCAC 
AAGAAGGAGG GCGACGACCT CTTCGATCGT CTCAACACGC AGGTGCTTAA TGAGCATCTC 
AAGGAGCTGA TGGAAGGTCT CACGGCCAAG GTATTCCGTA CGTACAACGC ATCGAAAACA 
CTGCAAAGTC AGCTGGATCT GTTGACCGAT CCGAGCGCCA CGGTACCGGA AAAGCTGTTG 
GCCTACAATC GCGCCAACCG TGCCGTGGCC ATCCTCTGTA ACCATCAGCG TTCCGTGCCC 
AAGAGCCACG AGAAGTCAAT GGAGAATCTG AAGGAGAAGA TCAAGGCCAA GCGGGAAGCC 
ATCGAAAAAT GCGAGTCCGA GTATCATTCG AGAGACGAGA AGAAGGGCAA ACAGCTGGAG 
CGCCTAAGGG ATCAGCTGAA GAAACTAGAA CTACAAGAGA CTGATAGAGA CGAGAATAAG 
ACCATTGCCC TGGGCACATC TAAGCTAAAC TATCTTGATC CACGCATATC GGTGGCTTGG 
TGTAAAAAGC ATGACGTGCC GATTGAGAAG ATATTCAACA AAACGCAAAG AACCAAATTT 
CTGTGGGCCG TTCATATGGC CGATGAGAAT TATCGTTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285267.1
CDS	50..1831
Translation	MLRSWVALRPFEKQSVCLVLGQRMLAVATVAGKRRRVRRKSVQEEQIRWWEEEKRADGVKWSTLEHKGPVFAPRYERVPRNVRFYYDGKPLELSEETEEAATFYAKMLNHDYCTKEVFNNNFFKDFRKSMTPREREIIKDFRKCNFQEMFNYFQAESEKRKAASKEEKLIKKNENEALMKEFGFCMIDGHKEKIGNFRLEPPGLFRGRGEHPKMGMIKRRIQASDVSINCGKDSKVPSPPPGSRWKEVRHDNTVTWLASWIENVQGQVKYIMLNPSSKLKGEKDHIKYETARRLDKVIDKIRATYRDEWKSKEMRVRQRAVALYFIDKLALRAGNEKDEDQADTVGCCSLRVEHVQLHKELNGKENVVVFDFPGKDSIRYYNEVEVEKRVFKNLELFMEHKKEGDDLFDRLNTQVLNEHLKELMEGLTAKVFRTYNASKTLQSQLDLLTDPSATVPEKLLAYNRANRAVAILCNHQRSVPKSHEKSMENLKEKIKAKREAIEKCESEYHSRDEKKGKQLERLRDQLKKLELQETDRDENKTIALGTSKLNYLDPRISVAWCKKHDVPIEKIFNKTQRTKFLWAVHMADENYRF

Target ORF information:

RefSeq Version	NM_001298338.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster topoisomerase 1, transcript variant E (Top1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298338.1

ORF Insert Sequence:

ATGTTGCGCT CTTGGGTGGC ATTGCGTCCA TTCGAAAAGC AGAGTGTCTG CCTGGTGCTC 
GGACAGCGTA TGTTGGCCGT AGCCACCGTT GCGGGCAAAC GGAGAAGAGT GCGACGGAAG 
AGCGTCCAGG AAGAGCAAAT TCGATGGTGG GAGGAGGAGA AGCGTGCCGA TGGCGTCAAA 
TGGTCAACGC TGGAGCACAA GGGACCCGTG TTTGCACCGC GATACGAACG GGTGCCACGC 
AATGTACGGT TCTACTACGA TGGCAAGCCG TTGGAGCTCT CTGAGGAAAC GGAGGAGGCA 
GCCACGTTCT ATGCCAAGAT GCTGAACCAC GACTACTGCA CCAAGGAAGT GTTCAATAAC 
AACTTCTTCA AGGACTTCCG CAAGTCCATG ACGCCCAGAG AGAGGGAGAT TATCAAGGAT 
TTCCGCAAGT GCAACTTCCA GGAGATGTTC AACTACTTCC AAGCGGAGTC CGAAAAGCGC 
AAGGCAGCCA GCAAGGAGGA GAAGCTGATC AAGAAGAACG AAAACGAGGC GCTGATGAAG 
GAATTCGGAT TCTGCATGAT TGATGGGCAC AAGGAGAAGA TCGGTAACTT CCGCCTGGAG 
CCGCCAGGCC TGTTCCGCGG TCGTGGCGAG CATCCCAAAA TGGGCATGAT CAAGCGGCGC 
ATCCAAGCCA GCGATGTGTC CATTAACTGT GGCAAAGATT CAAAGGTGCC TTCACCGCCG 
CCCGGATCTC GCTGGAAGGA GGTGCGCCAC GACAACACGG TCACCTGGCT GGCCTCCTGG 
ATCGAGAATG TGCAGGGACA GGTCAAGTAC ATCATGTTGA ATCCCTCCTC GAAACTCAAG 
GGCGAGAAGG ATCACATTAA GTACGAGACG GCGCGACGCC TGGACAAGGT AATCGATAAG 
ATACGTGCCA CCTATCGCGA CGAGTGGAAG TCCAAGGAGA TGAGGGTTCG CCAGCGAGCG 
GTGGCCCTTT ACTTCATCGA CAAACTGGCG CTGAGAGCAG GCAACGAAAA GGACGAGGAT 
CAGGCCGATA CCGTGGGCTG TTGCTCGCTA CGCGTGGAGC ATGTCCAGCT CCACAAGGAA 
CTGAACGGAA AGGAGAACGT GGTGGTCTTT GATTTCCCCG GTAAGGACTC CATTCGGTAT 
TACAACGAGG TTGAGGTGGA GAAGCGCGTC TTTAAGAACC TCGAACTGTT CATGGAGCAC 
AAGAAGGAGG GCGACGACCT CTTCGATCGT CTCAACACGC AGGTGCTTAA TGAGCATCTC 
AAGGAGCTGA TGGAAGGTCT CACGGCCAAG GTATTCCGTA CGTACAACGC ATCGAAAACA 
CTGCAAAGTC AGCTGGATCT GTTGACCGAT CCGAGCGCCA CGGTACCGGA AAAGCTGTTG 
GCCTACAATC GCGCCAACCG TGCCGTGGCC ATCCTCTGTA ACCATCAGCG TTCCGTGCCC 
AAGAGCCACG AGAAGTCAAT GGAGAATCTG AAGGAGAAGA TCAAGGCCAA GCGGGAAGCC 
ATCGAAAAAT GCGAGTCCGA GTATCATTCG AGAGACGAGA AGAAGGGCAA ACAGCTGGAG 
CGCCTAAGGG ATCAGCTGAA GAAACTAGAA CTACAAGAGA CTGATAGAGA CGAGAATAAG 
ACCATTGCCC TGGGCACATC TAAGCTAAAC TATCTTGATC CACGCATATC GGTGGCTTGG 
TGTAAAAAGC ATGACGTGCC GATTGAGAAG ATATTCAACA AAACGCAAAG AACCAAATTT 
CTGTGGGCCG TTCATATGGC CGATGAGAAT TATCGTTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Top1 ( NM_078606.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_511161.2 Drosophila melanogaster topoisomerase 1, transcript variant A (Top1), mRNA.

Price & Availability↑

CloneID	OFa01667
Clone ID Related Accession (Same CDS sequence)	NM_078606.4 , NM_001014742.2
Accession Version	NM_078606.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2925bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	topoisomerase 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_078606.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTGGGG ATGTGGCTGC CGAAAATAGC ATCCACATAC AAAATGGTGG CAGCTGCGAG 
GTGGTTCAAT CCAACGGGGT GACCACCAAT GGACACGGAC ACCACCACCA TCATCACCAC 
AGCAGCAGCA GCAGCAGCAG CAGCAAGCAT AAATCCTCCA GCAAGGACAA GCATCGTGAT 
AGGGAGAGGG AGCACAAGAG CTCCAATTCC TCCAGCTCGT CGAAGGAGCA CAAGAGCAGC 
AGTCGCGACA AGGATCGACA CAAGAGCAGC AGTTCGTCGT CAAAGCATCG GGACAAGGAC 
AAGGAGCGCG ATGGCAGCAG CAACTCGCAT CGCAGCGGCT CATCGTCCAG CCACAAGGAT 
AAGGATGGGA GCAGCAGCAG CAAGCACAAG TCGTCGTCGG GCCACCACAA GAGGTCCTCG 
AAGGATAAGG AGCGACGCGA TAAGGACAAG GATCGCGGCA GCAGTTCATC CAGTCGGCAC 
AAGTCCTCGT CCAGCTCCCG TGACAAAGAG CGCAGCAGCA GCAGTCACAA GAGCAGCTCC 
TCTTCATCCT CATCGAAGAG CAAACATTCC AGTTCGCGGC ACAGCAGCTC ATCTTCCTCG 
AAAGACCAGC CATCGTACGA TGGCGTATTT GTTAAGCCGG AGCCCGTTTC CCAACAGCTG 
ATGCACTCGG GCTCGGTGGA TGCTTTCCAG ATGCAACAGC TCGGTAGCTA CGAAGCCGCA 
GCCGCCGGCA CCAATTTTAA TGGCAATGGA AACGTTGCCG GTGCCAACTA TAAGAACGGC 
TACGAGGAAT CGATCGTAGA CATCAAGAAG GAGGAGGAGA GCTTTAACAA TCTGTCGCAA 
GCCAGCTCCT GTGACTACTC CATGTCCCAG TTTCGTGCCG ATGAGCCGCC GTTTGTGGTG 
AAGCACGAGC AGAGCTACGC CGAAGAGGAC AGCACCATGA ACTACAATGA TCACGACGAC 
GACGCCGACG AGATGAACGA CGACGAGGAG GATGTGCCGC TGGCCATGCG CAAGCGCAAA 
CAGGAGGCAA CCGATCGTCC TGATGGCGGC ATGGATAACG ACGATGACGA CGACATTCCG 
CTGCTGGCGC GCAAGAAGGT TAAGAAGGAG AAGATCAAGA AGGAGTCCAA GGAAAAGTCC 
AAGAAGCGGG TCAAGGAGGA GCCCAGCGAT GATTACGGCA ATGTCAAGCC GAAAAAGAAA 
AAGATGAAAA AGGAGCCCGA GCCTGCCGTA TCCCCCGGCA AACGACAGAA GGCAAAGGCC 
AAGGTGGAGG AGGAGGAGGT TTGGCGATGG TGGGAGGAGG AGAAGCGTGC CGATGGCGTC 
AAATGGTCAA CGCTGGAGCA CAAGGGACCC GTGTTTGCAC CGCGATACGA ACGGGTGCCA 
CGCAATGTAC GGTTCTACTA CGATGGCAAG CCGTTGGAGC TCTCTGAGGA AACGGAGGAG 
GCAGCCACGT TCTATGCCAA GATGCTGAAC CACGACTACT GCACCAAGGA AGTGTTCAAT 
AACAACTTCT TCAAGGACTT CCGCAAGTCC ATGACGCCCA GAGAGAGGGA GATTATCAAG 
GATTTCCGCA AGTGCAACTT CCAGGAGATG TTCAACTACT TCCAAGCGGA GTCCGAAAAG 
CGCAAGGCAG CCAGCAAGGA GGAGAAGCTG ATCAAGAAGA ACGAAAACGA GGCGCTGATG 
AAGGAATTCG GATTCTGCAT GATTGATGGG CACAAGGAGA AGATCGGTAA CTTCCGCCTG 
GAGCCGCCAG GCCTGTTCCG CGGTCGTGGC GAGCATCCCA AAATGGGCAT GATCAAGCGG 
CGCATCCAAG CCAGCGATGT GTCCATTAAC TGTGGCAAAG ATTCAAAGGT GCCTTCACCG 
CCGCCCGGAT CTCGCTGGAA GGAGGTGCGC CACGACAACA CGGTCACCTG GCTGGCCTCC 
TGGATCGAGA ATGTGCAGGG ACAGGTCAAG TACATCATGT TGAATCCCTC CTCGAAACTC 
AAGGGCGAGA AGGATCACAT TAAGTACGAG ACGGCGCGAC GCCTGGACAA GGTAATCGAT 
AAGATACGTG CCACCTATCG CGACGAGTGG AAGTCCAAGG AGATGAGGGT TCGCCAGCGA 
GCGGTGGCCC TTTACTTCAT CGACAAACTG GCGCTGAGAG CAGGCAACGA AAAGGACGAG 
GATCAGGCCG ATACCGTGGG CTGTTGCTCG CTACGCGTGG AGCATGTCCA GCTCCACAAG 
GAACTGAACG GAAAGGAGAA CGTGGTGGTC TTTGATTTCC CCGGTAAGGA CTCCATTCGG 
TATTACAACG AGGTTGAGGT GGAGAAGCGC GTCTTTAAGA ACCTCGAACT GTTCATGGAG 
CACAAGAAGG AGGGCGACGA CCTCTTCGAT CGTCTCAACA CGCAGGTGCT TAATGAGCAT 
CTCAAGGAGC TGATGGAAGG TCTCACGGCC AAGGTATTCC GTACGTACAA CGCATCGAAA 
ACACTGCAAA GTCAGCTGGA TCTGTTGACC GATCCGAGCG CCACGGTACC GGAAAAGCTG 
TTGGCCTACA ATCGCGCCAA CCGTGCCGTG GCCATCCTCT GTAACCATCA GCGTTCCGTG 
CCCAAGAGCC ACGAGAAGTC AATGGAGAAT CTGAAGGAGA AGATCAAGGC CAAGCGGGAA 
GCCATCGAAA AATGCGAGTC CGAGTATCAT TCGAGAGACG AGAAGAAGGG CAAACAGCTG 
GAGCGCCTAA GGGATCAGCT GAAGAAACTA GAACTACAAG AGACTGATAG AGACGAGAAT 
AAGACCATTG CCCTGGGCAC ATCTAAGCTA AACTATCTTG ATCCACGCAT ATCGGTGGCT 
TGGTGTAAAA AGCATGACGT GCCGATTGAG AAGATATTCA ACAAAACGCA AAGAACCAAA 
TTTCTGTGGG CCGTTCATAT GGCCGATGAG AATTATCGTT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_511161.2
CDS	303..3227
Translation	MSGDVAAENSIHIQNGGSCEVVQSNGVTTNGHGHHHHHHHSSSSSSSSKHKSSSKDKHRDREREHKSSNSSSSSKEHKSSSRDKDRHKSSSSSSKHRDKDKERDGSSNSHRSGSSSSHKDKDGSSSSKHKSSSGHHKRSSKDKERRDKDKDRGSSSSSRHKSSSSSRDKERSSSSHKSSSSSSSSKSKHSSSRHSSSSSSKDQPSYDGVFVKPEPVSQQLMHSGSVDAFQMQQLGSYEAAAAGTNFNGNGNVAGANYKNGYEESIVDIKKEEESFNNLSQASSCDYSMSQFRADEPPFVVKHEQSYAEEDSTMNYNDHDDDADEMNDDEEDVPLAMRKRKQEATDRPDGGMDNDDDDDIPLLARKKVKKEKIKKESKEKSKKRVKEEPSDDYGNVKPKKKKMKKEPEPAVSPGKRQKAKAKVEEEEVWRWWEEEKRADGVKWSTLEHKGPVFAPRYERVPRNVRFYYDGKPLELSEETEEAATFYAKMLNHDYCTKEVFNNNFFKDFRKSMTPREREIIKDFRKCNFQEMFNYFQAESEKRKAASKEEKLIKKNENEALMKEFGFCMIDGHKEKIGNFRLEPPGLFRGRGEHPKMGMIKRRIQASDVSINCGKDSKVPSPPPGSRWKEVRHDNTVTWLASWIENVQGQVKYIMLNPSSKLKGEKDHIKYETARRLDKVIDKIRATYRDEWKSKEMRVRQRAVALYFIDKLALRAGNEKDEDQADTVGCCSLRVEHVQLHKELNGKENVVVFDFPGKDSIRYYNEVEVEKRVFKNLELFMEHKKEGDDLFDRLNTQVLNEHLKELMEGLTAKVFRTYNASKTLQSQLDLLTDPSATVPEKLLAYNRANRAVAILCNHQRSVPKSHEKSMENLKEKIKAKREAIEKCESEYHSRDEKKGKQLERLRDQLKKLELQETDRDENKTIALGTSKLNYLDPRISVAWCKKHDVPIEKIFNKTQRTKFLWAVHMADENYRF

Target ORF information:

RefSeq Version	NM_078606.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster topoisomerase 1, transcript variant A (Top1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078606.4

ORF Insert Sequence:

ATGAGTGGGG ATGTGGCTGC CGAAAATAGC ATCCACATAC AAAATGGTGG CAGCTGCGAG 
GTGGTTCAAT CCAACGGGGT GACCACCAAT GGACACGGAC ACCACCACCA TCATCACCAC 
AGCAGCAGCA GCAGCAGCAG CAGCAAGCAT AAATCCTCCA GCAAGGACAA GCATCGTGAT 
AGGGAGAGGG AGCACAAGAG CTCCAATTCC TCCAGCTCGT CGAAGGAGCA CAAGAGCAGC 
AGTCGCGACA AGGATCGACA CAAGAGCAGC AGTTCGTCGT CAAAGCATCG GGACAAGGAC 
AAGGAGCGCG ATGGCAGCAG CAACTCGCAT CGCAGCGGCT CATCGTCCAG CCACAAGGAT 
AAGGATGGGA GCAGCAGCAG CAAGCACAAG TCGTCGTCGG GCCACCACAA GAGGTCCTCG 
AAGGATAAGG AGCGACGCGA TAAGGACAAG GATCGCGGCA GCAGTTCATC CAGTCGGCAC 
AAGTCCTCGT CCAGCTCCCG TGACAAAGAG CGCAGCAGCA GCAGTCACAA GAGCAGCTCC 
TCTTCATCCT CATCGAAGAG CAAACATTCC AGTTCGCGGC ACAGCAGCTC ATCTTCCTCG 
AAAGACCAGC CATCGTACGA TGGCGTATTT GTTAAGCCGG AGCCCGTTTC CCAACAGCTG 
ATGCACTCGG GCTCGGTGGA TGCTTTCCAG ATGCAACAGC TCGGTAGCTA CGAAGCCGCA 
GCCGCCGGCA CCAATTTTAA TGGCAATGGA AACGTTGCCG GTGCCAACTA TAAGAACGGC 
TACGAGGAAT CGATCGTAGA CATCAAGAAG GAGGAGGAGA GCTTTAACAA TCTGTCGCAA 
GCCAGCTCCT GTGACTACTC CATGTCCCAG TTTCGTGCCG ATGAGCCGCC GTTTGTGGTG 
AAGCACGAGC AGAGCTACGC CGAAGAGGAC AGCACCATGA ACTACAATGA TCACGACGAC 
GACGCCGACG AGATGAACGA CGACGAGGAG GATGTGCCGC TGGCCATGCG CAAGCGCAAA 
CAGGAGGCAA CCGATCGTCC TGATGGCGGC ATGGATAACG ACGATGACGA CGACATTCCG 
CTGCTGGCGC GCAAGAAGGT TAAGAAGGAG AAGATCAAGA AGGAGTCCAA GGAAAAGTCC 
AAGAAGCGGG TCAAGGAGGA GCCCAGCGAT GATTACGGCA ATGTCAAGCC GAAAAAGAAA 
AAGATGAAAA AGGAGCCCGA GCCTGCCGTA TCCCCCGGCA AACGACAGAA GGCAAAGGCC 
AAGGTGGAGG AGGAGGAGGT TTGGCGATGG TGGGAGGAGG AGAAGCGTGC CGATGGCGTC 
AAATGGTCAA CGCTGGAGCA CAAGGGACCC GTGTTTGCAC CGCGATACGA ACGGGTGCCA 
CGCAATGTAC GGTTCTACTA CGATGGCAAG CCGTTGGAGC TCTCTGAGGA AACGGAGGAG 
GCAGCCACGT TCTATGCCAA GATGCTGAAC CACGACTACT GCACCAAGGA AGTGTTCAAT 
AACAACTTCT TCAAGGACTT CCGCAAGTCC ATGACGCCCA GAGAGAGGGA GATTATCAAG 
GATTTCCGCA AGTGCAACTT CCAGGAGATG TTCAACTACT TCCAAGCGGA GTCCGAAAAG 
CGCAAGGCAG CCAGCAAGGA GGAGAAGCTG ATCAAGAAGA ACGAAAACGA GGCGCTGATG 
AAGGAATTCG GATTCTGCAT GATTGATGGG CACAAGGAGA AGATCGGTAA CTTCCGCCTG 
GAGCCGCCAG GCCTGTTCCG CGGTCGTGGC GAGCATCCCA AAATGGGCAT GATCAAGCGG 
CGCATCCAAG CCAGCGATGT GTCCATTAAC TGTGGCAAAG ATTCAAAGGT GCCTTCACCG 
CCGCCCGGAT CTCGCTGGAA GGAGGTGCGC CACGACAACA CGGTCACCTG GCTGGCCTCC 
TGGATCGAGA ATGTGCAGGG ACAGGTCAAG TACATCATGT TGAATCCCTC CTCGAAACTC 
AAGGGCGAGA AGGATCACAT TAAGTACGAG ACGGCGCGAC GCCTGGACAA GGTAATCGAT 
AAGATACGTG CCACCTATCG CGACGAGTGG AAGTCCAAGG AGATGAGGGT TCGCCAGCGA 
GCGGTGGCCC TTTACTTCAT CGACAAACTG GCGCTGAGAG CAGGCAACGA AAAGGACGAG 
GATCAGGCCG ATACCGTGGG CTGTTGCTCG CTACGCGTGG AGCATGTCCA GCTCCACAAG 
GAACTGAACG GAAAGGAGAA CGTGGTGGTC TTTGATTTCC CCGGTAAGGA CTCCATTCGG 
TATTACAACG AGGTTGAGGT GGAGAAGCGC GTCTTTAAGA ACCTCGAACT GTTCATGGAG 
CACAAGAAGG AGGGCGACGA CCTCTTCGAT CGTCTCAACA CGCAGGTGCT TAATGAGCAT 
CTCAAGGAGC TGATGGAAGG TCTCACGGCC AAGGTATTCC GTACGTACAA CGCATCGAAA 
ACACTGCAAA GTCAGCTGGA TCTGTTGACC GATCCGAGCG CCACGGTACC GGAAAAGCTG 
TTGGCCTACA ATCGCGCCAA CCGTGCCGTG GCCATCCTCT GTAACCATCA GCGTTCCGTG 
CCCAAGAGCC ACGAGAAGTC AATGGAGAAT CTGAAGGAGA AGATCAAGGC CAAGCGGGAA 
GCCATCGAAA AATGCGAGTC CGAGTATCAT TCGAGAGACG AGAAGAAGGG CAAACAGCTG 
GAGCGCCTAA GGGATCAGCT GAAGAAACTA GAACTACAAG AGACTGATAG AGACGAGAAT 
AAGACCATTG CCCTGGGCAC ATCTAAGCTA AACTATCTTG ATCCACGCAT ATCGGTGGCT 
TGGTGTAAAA AGCATGACGT GCCGATTGAG AAGATATTCA ACAAAACGCA AAGAACCAAA 
TTTCTGTGGG CCGTTCATAT GGCCGATGAG AATTATCGTT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Top1 ( NM_001014742.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001014742.1 Drosophila melanogaster topoisomerase 1, transcript variant C (Top1), mRNA.

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CloneID	OFa01667
Clone ID Related Accession (Same CDS sequence)	NM_078606.4 , NM_001014742.2
Accession Version	NM_001014742.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2925bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	topoisomerase 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001014742.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTGGGG ATGTGGCTGC CGAAAATAGC ATCCACATAC AAAATGGTGG CAGCTGCGAG 
GTGGTTCAAT CCAACGGGGT GACCACCAAT GGACACGGAC ACCACCACCA TCATCACCAC 
AGCAGCAGCA GCAGCAGCAG CAGCAAGCAT AAATCCTCCA GCAAGGACAA GCATCGTGAT 
AGGGAGAGGG AGCACAAGAG CTCCAATTCC TCCAGCTCGT CGAAGGAGCA CAAGAGCAGC 
AGTCGCGACA AGGATCGACA CAAGAGCAGC AGTTCGTCGT CAAAGCATCG GGACAAGGAC 
AAGGAGCGCG ATGGCAGCAG CAACTCGCAT CGCAGCGGCT CATCGTCCAG CCACAAGGAT 
AAGGATGGGA GCAGCAGCAG CAAGCACAAG TCGTCGTCGG GCCACCACAA GAGGTCCTCG 
AAGGATAAGG AGCGACGCGA TAAGGACAAG GATCGCGGCA GCAGTTCATC CAGTCGGCAC 
AAGTCCTCGT CCAGCTCCCG TGACAAAGAG CGCAGCAGCA GCAGTCACAA GAGCAGCTCC 
TCTTCATCCT CATCGAAGAG CAAACATTCC AGTTCGCGGC ACAGCAGCTC ATCTTCCTCG 
AAAGACCAGC CATCGTACGA TGGCGTATTT GTTAAGCCGG AGCCCGTTTC CCAACAGCTG 
ATGCACTCGG GCTCGGTGGA TGCTTTCCAG ATGCAACAGC TCGGTAGCTA CGAAGCCGCA 
GCCGCCGGCA CCAATTTTAA TGGCAATGGA AACGTTGCCG GTGCCAACTA TAAGAACGGC 
TACGAGGAAT CGATCGTAGA CATCAAGAAG GAGGAGGAGA GCTTTAACAA TCTGTCGCAA 
GCCAGCTCCT GTGACTACTC CATGTCCCAG TTTCGTGCCG ATGAGCCGCC GTTTGTGGTG 
AAGCACGAGC AGAGCTACGC CGAAGAGGAC AGCACCATGA ACTACAATGA TCACGACGAC 
GACGCCGACG AGATGAACGA CGACGAGGAG GATGTGCCGC TGGCCATGCG CAAGCGCAAA 
CAGGAGGCAA CCGATCGTCC TGATGGCGGC ATGGATAACG ACGATGACGA CGACATTCCG 
CTGCTGGCGC GCAAGAAGGT TAAGAAGGAG AAGATCAAGA AGGAGTCCAA GGAAAAGTCC 
AAGAAGCGGG TCAAGGAGGA GCCCAGCGAT GATTACGGCA ATGTCAAGCC GAAAAAGAAA 
AAGATGAAAA AGGAGCCCGA GCCTGCCGTA TCCCCCGGCA AACGACAGAA GGCAAAGGCC 
AAGGTGGAGG AGGAGGAGGT TTGGCGATGG TGGGAGGAGG AGAAGCGTGC CGATGGCGTC 
AAATGGTCAA CGCTGGAGCA CAAGGGACCC GTGTTTGCAC CGCGATACGA ACGGGTGCCA 
CGCAATGTAC GGTTCTACTA CGATGGCAAG CCGTTGGAGC TCTCTGAGGA AACGGAGGAG 
GCAGCCACGT TCTATGCCAA GATGCTGAAC CACGACTACT GCACCAAGGA AGTGTTCAAT 
AACAACTTCT TCAAGGACTT CCGCAAGTCC ATGACGCCCA GAGAGAGGGA GATTATCAAG 
GATTTCCGCA AGTGCAACTT CCAGGAGATG TTCAACTACT TCCAAGCGGA GTCCGAAAAG 
CGCAAGGCAG CCAGCAAGGA GGAGAAGCTG ATCAAGAAGA ACGAAAACGA GGCGCTGATG 
AAGGAATTCG GATTCTGCAT GATTGATGGG CACAAGGAGA AGATCGGTAA CTTCCGCCTG 
GAGCCGCCAG GCCTGTTCCG CGGTCGTGGC GAGCATCCCA AAATGGGCAT GATCAAGCGG 
CGCATCCAAG CCAGCGATGT GTCCATTAAC TGTGGCAAAG ATTCAAAGGT GCCTTCACCG 
CCGCCCGGAT CTCGCTGGAA GGAGGTGCGC CACGACAACA CGGTCACCTG GCTGGCCTCC 
TGGATCGAGA ATGTGCAGGG ACAGGTCAAG TACATCATGT TGAATCCCTC CTCGAAACTC 
AAGGGCGAGA AGGATCACAT TAAGTACGAG ACGGCGCGAC GCCTGGACAA GGTAATCGAT 
AAGATACGTG CCACCTATCG CGACGAGTGG AAGTCCAAGG AGATGAGGGT TCGCCAGCGA 
GCGGTGGCCC TTTACTTCAT CGACAAACTG GCGCTGAGAG CAGGCAACGA AAAGGACGAG 
GATCAGGCCG ATACCGTGGG CTGTTGCTCG CTACGCGTGG AGCATGTCCA GCTCCACAAG 
GAACTGAACG GAAAGGAGAA CGTGGTGGTC TTTGATTTCC CCGGTAAGGA CTCCATTCGG 
TATTACAACG AGGTTGAGGT GGAGAAGCGC GTCTTTAAGA ACCTCGAACT GTTCATGGAG 
CACAAGAAGG AGGGCGACGA CCTCTTCGAT CGTCTCAACA CGCAGGTGCT TAATGAGCAT 
CTCAAGGAGC TGATGGAAGG TCTCACGGCC AAGGTATTCC GTACGTACAA CGCATCGAAA 
ACACTGCAAA GTCAGCTGGA TCTGTTGACC GATCCGAGCG CCACGGTACC GGAAAAGCTG 
TTGGCCTACA ATCGCGCCAA CCGTGCCGTG GCCATCCTCT GTAACCATCA GCGTTCCGTG 
CCCAAGAGCC ACGAGAAGTC AATGGAGAAT CTGAAGGAGA AGATCAAGGC CAAGCGGGAA 
GCCATCGAAA AATGCGAGTC CGAGTATCAT TCGAGAGACG AGAAGAAGGG CAAACAGCTG 
GAGCGCCTAA GGGATCAGCT GAAGAAACTA GAACTACAAG AGACTGATAG AGACGAGAAT 
AAGACCATTG CCCTGGGCAC ATCTAAGCTA AACTATCTTG ATCCACGCAT ATCGGTGGCT 
TGGTGTAAAA AGCATGACGT GCCGATTGAG AAGATATTCA ACAAAACGCA AAGAACCAAA 
TTTCTGTGGG CCGTTCATAT GGCCGATGAG AATTATCGTT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014742.1
CDS	290..3214
Translation	MSGDVAAENSIHIQNGGSCEVVQSNGVTTNGHGHHHHHHHSSSSSSSSKHKSSSKDKHRDREREHKSSNSSSSSKEHKSSSRDKDRHKSSSSSSKHRDKDKERDGSSNSHRSGSSSSHKDKDGSSSSKHKSSSGHHKRSSKDKERRDKDKDRGSSSSSRHKSSSSSRDKERSSSSHKSSSSSSSSKSKHSSSRHSSSSSSKDQPSYDGVFVKPEPVSQQLMHSGSVDAFQMQQLGSYEAAAAGTNFNGNGNVAGANYKNGYEESIVDIKKEEESFNNLSQASSCDYSMSQFRADEPPFVVKHEQSYAEEDSTMNYNDHDDDADEMNDDEEDVPLAMRKRKQEATDRPDGGMDNDDDDDIPLLARKKVKKEKIKKESKEKSKKRVKEEPSDDYGNVKPKKKKMKKEPEPAVSPGKRQKAKAKVEEEEVWRWWEEEKRADGVKWSTLEHKGPVFAPRYERVPRNVRFYYDGKPLELSEETEEAATFYAKMLNHDYCTKEVFNNNFFKDFRKSMTPREREIIKDFRKCNFQEMFNYFQAESEKRKAASKEEKLIKKNENEALMKEFGFCMIDGHKEKIGNFRLEPPGLFRGRGEHPKMGMIKRRIQASDVSINCGKDSKVPSPPPGSRWKEVRHDNTVTWLASWIENVQGQVKYIMLNPSSKLKGEKDHIKYETARRLDKVIDKIRATYRDEWKSKEMRVRQRAVALYFIDKLALRAGNEKDEDQADTVGCCSLRVEHVQLHKELNGKENVVVFDFPGKDSIRYYNEVEVEKRVFKNLELFMEHKKEGDDLFDRLNTQVLNEHLKELMEGLTAKVFRTYNASKTLQSQLDLLTDPSATVPEKLLAYNRANRAVAILCNHQRSVPKSHEKSMENLKEKIKAKREAIEKCESEYHSRDEKKGKQLERLRDQLKKLELQETDRDENKTIALGTSKLNYLDPRISVAWCKKHDVPIEKIFNKTQRTKFLWAVHMADENYRF

Target ORF information:

RefSeq Version	NM_001014742.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster topoisomerase 1, transcript variant C (Top1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014742.2

ORF Insert Sequence:

ATGAGTGGGG ATGTGGCTGC CGAAAATAGC ATCCACATAC AAAATGGTGG CAGCTGCGAG 
GTGGTTCAAT CCAACGGGGT GACCACCAAT GGACACGGAC ACCACCACCA TCATCACCAC 
AGCAGCAGCA GCAGCAGCAG CAGCAAGCAT AAATCCTCCA GCAAGGACAA GCATCGTGAT 
AGGGAGAGGG AGCACAAGAG CTCCAATTCC TCCAGCTCGT CGAAGGAGCA CAAGAGCAGC 
AGTCGCGACA AGGATCGACA CAAGAGCAGC AGTTCGTCGT CAAAGCATCG GGACAAGGAC 
AAGGAGCGCG ATGGCAGCAG CAACTCGCAT CGCAGCGGCT CATCGTCCAG CCACAAGGAT 
AAGGATGGGA GCAGCAGCAG CAAGCACAAG TCGTCGTCGG GCCACCACAA GAGGTCCTCG 
AAGGATAAGG AGCGACGCGA TAAGGACAAG GATCGCGGCA GCAGTTCATC CAGTCGGCAC 
AAGTCCTCGT CCAGCTCCCG TGACAAAGAG CGCAGCAGCA GCAGTCACAA GAGCAGCTCC 
TCTTCATCCT CATCGAAGAG CAAACATTCC AGTTCGCGGC ACAGCAGCTC ATCTTCCTCG 
AAAGACCAGC CATCGTACGA TGGCGTATTT GTTAAGCCGG AGCCCGTTTC CCAACAGCTG 
ATGCACTCGG GCTCGGTGGA TGCTTTCCAG ATGCAACAGC TCGGTAGCTA CGAAGCCGCA 
GCCGCCGGCA CCAATTTTAA TGGCAATGGA AACGTTGCCG GTGCCAACTA TAAGAACGGC 
TACGAGGAAT CGATCGTAGA CATCAAGAAG GAGGAGGAGA GCTTTAACAA TCTGTCGCAA 
GCCAGCTCCT GTGACTACTC CATGTCCCAG TTTCGTGCCG ATGAGCCGCC GTTTGTGGTG 
AAGCACGAGC AGAGCTACGC CGAAGAGGAC AGCACCATGA ACTACAATGA TCACGACGAC 
GACGCCGACG AGATGAACGA CGACGAGGAG GATGTGCCGC TGGCCATGCG CAAGCGCAAA 
CAGGAGGCAA CCGATCGTCC TGATGGCGGC ATGGATAACG ACGATGACGA CGACATTCCG 
CTGCTGGCGC GCAAGAAGGT TAAGAAGGAG AAGATCAAGA AGGAGTCCAA GGAAAAGTCC 
AAGAAGCGGG TCAAGGAGGA GCCCAGCGAT GATTACGGCA ATGTCAAGCC GAAAAAGAAA 
AAGATGAAAA AGGAGCCCGA GCCTGCCGTA TCCCCCGGCA AACGACAGAA GGCAAAGGCC 
AAGGTGGAGG AGGAGGAGGT TTGGCGATGG TGGGAGGAGG AGAAGCGTGC CGATGGCGTC 
AAATGGTCAA CGCTGGAGCA CAAGGGACCC GTGTTTGCAC CGCGATACGA ACGGGTGCCA 
CGCAATGTAC GGTTCTACTA CGATGGCAAG CCGTTGGAGC TCTCTGAGGA AACGGAGGAG 
GCAGCCACGT TCTATGCCAA GATGCTGAAC CACGACTACT GCACCAAGGA AGTGTTCAAT 
AACAACTTCT TCAAGGACTT CCGCAAGTCC ATGACGCCCA GAGAGAGGGA GATTATCAAG 
GATTTCCGCA AGTGCAACTT CCAGGAGATG TTCAACTACT TCCAAGCGGA GTCCGAAAAG 
CGCAAGGCAG CCAGCAAGGA GGAGAAGCTG ATCAAGAAGA ACGAAAACGA GGCGCTGATG 
AAGGAATTCG GATTCTGCAT GATTGATGGG CACAAGGAGA AGATCGGTAA CTTCCGCCTG 
GAGCCGCCAG GCCTGTTCCG CGGTCGTGGC GAGCATCCCA AAATGGGCAT GATCAAGCGG 
CGCATCCAAG CCAGCGATGT GTCCATTAAC TGTGGCAAAG ATTCAAAGGT GCCTTCACCG 
CCGCCCGGAT CTCGCTGGAA GGAGGTGCGC CACGACAACA CGGTCACCTG GCTGGCCTCC 
TGGATCGAGA ATGTGCAGGG ACAGGTCAAG TACATCATGT TGAATCCCTC CTCGAAACTC 
AAGGGCGAGA AGGATCACAT TAAGTACGAG ACGGCGCGAC GCCTGGACAA GGTAATCGAT 
AAGATACGTG CCACCTATCG CGACGAGTGG AAGTCCAAGG AGATGAGGGT TCGCCAGCGA 
GCGGTGGCCC TTTACTTCAT CGACAAACTG GCGCTGAGAG CAGGCAACGA AAAGGACGAG 
GATCAGGCCG ATACCGTGGG CTGTTGCTCG CTACGCGTGG AGCATGTCCA GCTCCACAAG 
GAACTGAACG GAAAGGAGAA CGTGGTGGTC TTTGATTTCC CCGGTAAGGA CTCCATTCGG 
TATTACAACG AGGTTGAGGT GGAGAAGCGC GTCTTTAAGA ACCTCGAACT GTTCATGGAG 
CACAAGAAGG AGGGCGACGA CCTCTTCGAT CGTCTCAACA CGCAGGTGCT TAATGAGCAT 
CTCAAGGAGC TGATGGAAGG TCTCACGGCC AAGGTATTCC GTACGTACAA CGCATCGAAA 
ACACTGCAAA GTCAGCTGGA TCTGTTGACC GATCCGAGCG CCACGGTACC GGAAAAGCTG 
TTGGCCTACA ATCGCGCCAA CCGTGCCGTG GCCATCCTCT GTAACCATCA GCGTTCCGTG 
CCCAAGAGCC ACGAGAAGTC AATGGAGAAT CTGAAGGAGA AGATCAAGGC CAAGCGGGAA 
GCCATCGAAA AATGCGAGTC CGAGTATCAT TCGAGAGACG AGAAGAAGGG CAAACAGCTG 
GAGCGCCTAA GGGATCAGCT GAAGAAACTA GAACTACAAG AGACTGATAG AGACGAGAAT 
AAGACCATTG CCCTGGGCAC ATCTAAGCTA AACTATCTTG ATCCACGCAT ATCGGTGGCT 
TGGTGTAAAA AGCATGACGT GCCGATTGAG AAGATATTCA ACAAAACGCA AAGAACCAAA 
TTTCTGTGGG CCGTTCATAT GGCCGATGAG AATTATCGTT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Top1 ( NM_001258750.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245679.1 Drosophila melanogaster topoisomerase 1, transcript variant D (Top1), mRNA.

Price & Availability↑

CloneID	OFa02721
Clone ID Related Accession (Same CDS sequence)	NM_001258750.2
Accession Version	NM_001258750.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2913bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	topoisomerase 1, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001258750.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGGATG CACAGAGCAT CCACATACAA AATGGTGGCA GCTGCGAGGT GGTTCAATCC 
AACGGGGTGA CCACCAATGG ACACGGACAC CACCACCATC ATCACCACAG CAGCAGCAGC 
AGCAGCAGCA GCAAGCATAA ATCCTCCAGC AAGGACAAGC ATCGTGATAG GGAGAGGGAG 
CACAAGAGCT CCAATTCCTC CAGCTCGTCG AAGGAGCACA AGAGCAGCAG TCGCGACAAG 
GATCGACACA AGAGCAGCAG TTCGTCGTCA AAGCATCGGG ACAAGGACAA GGAGCGCGAT 
GGCAGCAGCA ACTCGCATCG CAGCGGCTCA TCGTCCAGCC ACAAGGATAA GGATGGGAGC 
AGCAGCAGCA AGCACAAGTC GTCGTCGGGC CACCACAAGA GGTCCTCGAA GGATAAGGAG 
CGACGCGATA AGGACAAGGA TCGCGGCAGC AGTTCATCCA GTCGGCACAA GTCCTCGTCC 
AGCTCCCGTG ACAAAGAGCG CAGCAGCAGC AGTCACAAGA GCAGCTCCTC TTCATCCTCA 
TCGAAGAGCA AACATTCCAG TTCGCGGCAC AGCAGCTCAT CTTCCTCGAA AGACCAGCCA 
TCGTACGATG GCGTATTTGT TAAGCCGGAG CCCGTTTCCC AACAGCTGAT GCACTCGGGC 
TCGGTGGATG CTTTCCAGAT GCAACAGCTC GGTAGCTACG AAGCCGCAGC CGCCGGCACC 
AATTTTAATG GCAATGGAAA CGTTGCCGGT GCCAACTATA AGAACGGCTA CGAGGAATCG 
ATCGTAGACA TCAAGAAGGA GGAGGAGAGC TTTAACAATC TGTCGCAAGC CAGCTCCTGT 
GACTACTCCA TGTCCCAGTT TCGTGCCGAT GAGCCGCCGT TTGTGGTGAA GCACGAGCAG 
AGCTACGCCG AAGAGGACAG CACCATGAAC TACAATGATC ACGACGACGA CGCCGACGAG 
ATGAACGACG ACGAGGAGGA TGTGCCGCTG GCCATGCGCA AGCGCAAACA GGAGGCAACC 
GATCGTCCTG ATGGCGGCAT GGATAACGAC GATGACGACG ACATTCCGCT GCTGGCGCGC 
AAGAAGGTTA AGAAGGAGAA GATCAAGAAG GAGTCCAAGG AAAAGTCCAA GAAGCGGGTC 
AAGGAGGAGC CCAGCGATGA TTACGGCAAT GTCAAGCCGA AAAAGAAAAA GATGAAAAAG 
GAGCCCGAGC CTGCCGTATC CCCCGGCAAA CGACAGAAGG CAAAGGCCAA GGTGGAGGAG 
GAGGAGGTTT GGCGATGGTG GGAGGAGGAG AAGCGTGCCG ATGGCGTCAA ATGGTCAACG 
CTGGAGCACA AGGGACCCGT GTTTGCACCG CGATACGAAC GGGTGCCACG CAATGTACGG 
TTCTACTACG ATGGCAAGCC GTTGGAGCTC TCTGAGGAAA CGGAGGAGGC AGCCACGTTC 
TATGCCAAGA TGCTGAACCA CGACTACTGC ACCAAGGAAG TGTTCAATAA CAACTTCTTC 
AAGGACTTCC GCAAGTCCAT GACGCCCAGA GAGAGGGAGA TTATCAAGGA TTTCCGCAAG 
TGCAACTTCC AGGAGATGTT CAACTACTTC CAAGCGGAGT CCGAAAAGCG CAAGGCAGCC 
AGCAAGGAGG AGAAGCTGAT CAAGAAGAAC GAAAACGAGG CGCTGATGAA GGAATTCGGA 
TTCTGCATGA TTGATGGGCA CAAGGAGAAG ATCGGTAACT TCCGCCTGGA GCCGCCAGGC 
CTGTTCCGCG GTCGTGGCGA GCATCCCAAA ATGGGCATGA TCAAGCGGCG CATCCAAGCC 
AGCGATGTGT CCATTAACTG TGGCAAAGAT TCAAAGGTGC CTTCACCGCC GCCCGGATCT 
CGCTGGAAGG AGGTGCGCCA CGACAACACG GTCACCTGGC TGGCCTCCTG GATCGAGAAT 
GTGCAGGGAC AGGTCAAGTA CATCATGTTG AATCCCTCCT CGAAACTCAA GGGCGAGAAG 
GATCACATTA AGTACGAGAC GGCGCGACGC CTGGACAAGG TAATCGATAA GATACGTGCC 
ACCTATCGCG ACGAGTGGAA GTCCAAGGAG ATGAGGGTTC GCCAGCGAGC GGTGGCCCTT 
TACTTCATCG ACAAACTGGC GCTGAGAGCA GGCAACGAAA AGGACGAGGA TCAGGCCGAT 
ACCGTGGGCT GTTGCTCGCT ACGCGTGGAG CATGTCCAGC TCCACAAGGA ACTGAACGGA 
AAGGAGAACG TGGTGGTCTT TGATTTCCCC GGTAAGGACT CCATTCGGTA TTACAACGAG 
GTTGAGGTGG AGAAGCGCGT CTTTAAGAAC CTCGAACTGT TCATGGAGCA CAAGAAGGAG 
GGCGACGACC TCTTCGATCG TCTCAACACG CAGGTGCTTA ATGAGCATCT CAAGGAGCTG 
ATGGAAGGTC TCACGGCCAA GGTATTCCGT ACGTACAACG CATCGAAAAC ACTGCAAAGT 
CAGCTGGATC TGTTGACCGA TCCGAGCGCC ACGGTACCGG AAAAGCTGTT GGCCTACAAT 
CGCGCCAACC GTGCCGTGGC CATCCTCTGT AACCATCAGC GTTCCGTGCC CAAGAGCCAC 
GAGAAGTCAA TGGAGAATCT GAAGGAGAAG ATCAAGGCCA AGCGGGAAGC CATCGAAAAA 
TGCGAGTCCG AGTATCATTC GAGAGACGAG AAGAAGGGCA AACAGCTGGA GCGCCTAAGG 
GATCAGCTGA AGAAACTAGA ACTACAAGAG ACTGATAGAG ACGAGAATAA GACCATTGCC 
CTGGGCACAT CTAAGCTAAA CTATCTTGAT CCACGCATAT CGGTGGCTTG GTGTAAAAAG 
CATGACGTGC CGATTGAGAA GATATTCAAC AAAACGCAAA GAACCAAATT TCTGTGGGCC 
GTTCATATGG CCGATGAGAA TTATCGTTTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245679.1
CDS	650..3562
Translation	MTDAQSIHIQNGGSCEVVQSNGVTTNGHGHHHHHHHSSSSSSSSKHKSSSKDKHRDREREHKSSNSSSSSKEHKSSSRDKDRHKSSSSSSKHRDKDKERDGSSNSHRSGSSSSHKDKDGSSSSKHKSSSGHHKRSSKDKERRDKDKDRGSSSSSRHKSSSSSRDKERSSSSHKSSSSSSSSKSKHSSSRHSSSSSSKDQPSYDGVFVKPEPVSQQLMHSGSVDAFQMQQLGSYEAAAAGTNFNGNGNVAGANYKNGYEESIVDIKKEEESFNNLSQASSCDYSMSQFRADEPPFVVKHEQSYAEEDSTMNYNDHDDDADEMNDDEEDVPLAMRKRKQEATDRPDGGMDNDDDDDIPLLARKKVKKEKIKKESKEKSKKRVKEEPSDDYGNVKPKKKKMKKEPEPAVSPGKRQKAKAKVEEEEVWRWWEEEKRADGVKWSTLEHKGPVFAPRYERVPRNVRFYYDGKPLELSEETEEAATFYAKMLNHDYCTKEVFNNNFFKDFRKSMTPREREIIKDFRKCNFQEMFNYFQAESEKRKAASKEEKLIKKNENEALMKEFGFCMIDGHKEKIGNFRLEPPGLFRGRGEHPKMGMIKRRIQASDVSINCGKDSKVPSPPPGSRWKEVRHDNTVTWLASWIENVQGQVKYIMLNPSSKLKGEKDHIKYETARRLDKVIDKIRATYRDEWKSKEMRVRQRAVALYFIDKLALRAGNEKDEDQADTVGCCSLRVEHVQLHKELNGKENVVVFDFPGKDSIRYYNEVEVEKRVFKNLELFMEHKKEGDDLFDRLNTQVLNEHLKELMEGLTAKVFRTYNASKTLQSQLDLLTDPSATVPEKLLAYNRANRAVAILCNHQRSVPKSHEKSMENLKEKIKAKREAIEKCESEYHSRDEKKGKQLERLRDQLKKLELQETDRDENKTIALGTSKLNYLDPRISVAWCKKHDVPIEKIFNKTQRTKFLWAVHMADENYRF

Target ORF information:

RefSeq Version	NM_001258750.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster topoisomerase 1, transcript variant D (Top1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258750.2

ORF Insert Sequence:

ATGACGGATG CACAGAGCAT CCACATACAA AATGGTGGCA GCTGCGAGGT GGTTCAATCC 
AACGGGGTGA CCACCAATGG ACACGGACAC CACCACCATC ATCACCACAG CAGCAGCAGC 
AGCAGCAGCA GCAAGCATAA ATCCTCCAGC AAGGACAAGC ATCGTGATAG GGAGAGGGAG 
CACAAGAGCT CCAATTCCTC CAGCTCGTCG AAGGAGCACA AGAGCAGCAG TCGCGACAAG 
GATCGACACA AGAGCAGCAG TTCGTCGTCA AAGCATCGGG ACAAGGACAA GGAGCGCGAT 
GGCAGCAGCA ACTCGCATCG CAGCGGCTCA TCGTCCAGCC ACAAGGATAA GGATGGGAGC 
AGCAGCAGCA AGCACAAGTC GTCGTCGGGC CACCACAAGA GGTCCTCGAA GGATAAGGAG 
CGACGCGATA AGGACAAGGA TCGCGGCAGC AGTTCATCCA GTCGGCACAA GTCCTCGTCC 
AGCTCCCGTG ACAAAGAGCG CAGCAGCAGC AGTCACAAGA GCAGCTCCTC TTCATCCTCA 
TCGAAGAGCA AACATTCCAG TTCGCGGCAC AGCAGCTCAT CTTCCTCGAA AGACCAGCCA 
TCGTACGATG GCGTATTTGT TAAGCCGGAG CCCGTTTCCC AACAGCTGAT GCACTCGGGC 
TCGGTGGATG CTTTCCAGAT GCAACAGCTC GGTAGCTACG AAGCCGCAGC CGCCGGCACC 
AATTTTAATG GCAATGGAAA CGTTGCCGGT GCCAACTATA AGAACGGCTA CGAGGAATCG 
ATCGTAGACA TCAAGAAGGA GGAGGAGAGC TTTAACAATC TGTCGCAAGC CAGCTCCTGT 
GACTACTCCA TGTCCCAGTT TCGTGCCGAT GAGCCGCCGT TTGTGGTGAA GCACGAGCAG 
AGCTACGCCG AAGAGGACAG CACCATGAAC TACAATGATC ACGACGACGA CGCCGACGAG 
ATGAACGACG ACGAGGAGGA TGTGCCGCTG GCCATGCGCA AGCGCAAACA GGAGGCAACC 
GATCGTCCTG ATGGCGGCAT GGATAACGAC GATGACGACG ACATTCCGCT GCTGGCGCGC 
AAGAAGGTTA AGAAGGAGAA GATCAAGAAG GAGTCCAAGG AAAAGTCCAA GAAGCGGGTC 
AAGGAGGAGC CCAGCGATGA TTACGGCAAT GTCAAGCCGA AAAAGAAAAA GATGAAAAAG 
GAGCCCGAGC CTGCCGTATC CCCCGGCAAA CGACAGAAGG CAAAGGCCAA GGTGGAGGAG 
GAGGAGGTTT GGCGATGGTG GGAGGAGGAG AAGCGTGCCG ATGGCGTCAA ATGGTCAACG 
CTGGAGCACA AGGGACCCGT GTTTGCACCG CGATACGAAC GGGTGCCACG CAATGTACGG 
TTCTACTACG ATGGCAAGCC GTTGGAGCTC TCTGAGGAAA CGGAGGAGGC AGCCACGTTC 
TATGCCAAGA TGCTGAACCA CGACTACTGC ACCAAGGAAG TGTTCAATAA CAACTTCTTC 
AAGGACTTCC GCAAGTCCAT GACGCCCAGA GAGAGGGAGA TTATCAAGGA TTTCCGCAAG 
TGCAACTTCC AGGAGATGTT CAACTACTTC CAAGCGGAGT CCGAAAAGCG CAAGGCAGCC 
AGCAAGGAGG AGAAGCTGAT CAAGAAGAAC GAAAACGAGG CGCTGATGAA GGAATTCGGA 
TTCTGCATGA TTGATGGGCA CAAGGAGAAG ATCGGTAACT TCCGCCTGGA GCCGCCAGGC 
CTGTTCCGCG GTCGTGGCGA GCATCCCAAA ATGGGCATGA TCAAGCGGCG CATCCAAGCC 
AGCGATGTGT CCATTAACTG TGGCAAAGAT TCAAAGGTGC CTTCACCGCC GCCCGGATCT 
CGCTGGAAGG AGGTGCGCCA CGACAACACG GTCACCTGGC TGGCCTCCTG GATCGAGAAT 
GTGCAGGGAC AGGTCAAGTA CATCATGTTG AATCCCTCCT CGAAACTCAA GGGCGAGAAG 
GATCACATTA AGTACGAGAC GGCGCGACGC CTGGACAAGG TAATCGATAA GATACGTGCC 
ACCTATCGCG ACGAGTGGAA GTCCAAGGAG ATGAGGGTTC GCCAGCGAGC GGTGGCCCTT 
TACTTCATCG ACAAACTGGC GCTGAGAGCA GGCAACGAAA AGGACGAGGA TCAGGCCGAT 
ACCGTGGGCT GTTGCTCGCT ACGCGTGGAG CATGTCCAGC TCCACAAGGA ACTGAACGGA 
AAGGAGAACG TGGTGGTCTT TGATTTCCCC GGTAAGGACT CCATTCGGTA TTACAACGAG 
GTTGAGGTGG AGAAGCGCGT CTTTAAGAAC CTCGAACTGT TCATGGAGCA CAAGAAGGAG 
GGCGACGACC TCTTCGATCG TCTCAACACG CAGGTGCTTA ATGAGCATCT CAAGGAGCTG 
ATGGAAGGTC TCACGGCCAA GGTATTCCGT ACGTACAACG CATCGAAAAC ACTGCAAAGT 
CAGCTGGATC TGTTGACCGA TCCGAGCGCC ACGGTACCGG AAAAGCTGTT GGCCTACAAT 
CGCGCCAACC GTGCCGTGGC CATCCTCTGT AACCATCAGC GTTCCGTGCC CAAGAGCCAC 
GAGAAGTCAA TGGAGAATCT GAAGGAGAAG ATCAAGGCCA AGCGGGAAGC CATCGAAAAA 
TGCGAGTCCG AGTATCATTC GAGAGACGAG AAGAAGGGCA AACAGCTGGA GCGCCTAAGG 
GATCAGCTGA AGAAACTAGA ACTACAAGAG ACTGATAGAG ACGAGAATAA GACCATTGCC 
CTGGGCACAT CTAAGCTAAA CTATCTTGAT CCACGCATAT CGGTGGCTTG GTGTAAAAAG 
CATGACGTGC CGATTGAGAA GATATTCAAC AAAACGCAAA GAACCAAATT TCTGTGGGCC 
GTTCATATGG CCGATGAGAA TTATCGTTTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Top1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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