Eo cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Eo
Entrez Gene ID	32423
Full Name	Ecdysone oxidase
Synonyms	CG9504,DmEO,Dmel\CG9504,EO,EObeta1
General protein information	Preferred Names Ecdysone oxidase Names CG9504 gene product from transcript CG9504-RA CG9504-PA Eo-PA ecdysone oxidase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 13A1-13A1

mRNA and Protein(s)

mRNA	Protein	Name
NM_132758.2	NP_572986.1	ecdysone oxidase

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Eo gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Eo cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa01627	NM_132758.2 Latest version!	Drosophila melanogaster ecdysone oxidase (Eo), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Eo ( NM_132758.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572986.1 Drosophila melanogaster ecdysone oxidase (Eo), mRNA.

Price & Availability↑

CloneID	OFa01627
Clone ID Related Accession (Same CDS sequence)	NM_132758.2
Accession Version	NM_132758.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ecdysone oxidase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_132758.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCGATC AGCGCGTACA CAAACCGCGG ACAGCGAAGA CAAGAACCCT TCGCGTCAAT 
AGGATATTAT TACCGATGTT GCTTCTGTTT TCAGGAGAAA CTACATCAGT TGTGACCCAA 
TTGTTTTCGG TGGATAGCAG TGGCCTCGGC ATTTCGCTGA TGCAATCGGT GGCCATAGCT 
CTGAATGCAT CCAGCCTCGC CCTGGCCAAC AACACCGCCT GGCCGCTGCA ACACGAACCG 
CCAGAGGATC GGTTGGAGAT CGAGAGCTAT GACTATATAG TGGTGGGTGC AGGTAGTGCC 
GGATCCATTG TGGCCAGTCG ATTGAGTGAG CTGTGCCAGG TGAAGGTCCT GCTGCTGGAG 
GAGGGACAAT TACCACCGCT GGAATCGGAA ATCTTTGGTC TGACCGGTGC ACTGCATCAT 
GACGAGCGGT ACATGTTCCT GGAGGAAGCA GTGCCCAATC CGAAATGCTG CCAGGCAATG 
GCATCCATGC ACGGCTGCGT TTGGTGGCAT GGTCGCATGA TGGGCGGTGG TGGTGCCATT 
AATGGCAATA TCTTTATACC CGGCAGTCGG GAGAACTTCA GACGCTGGAA TTCAACCGGC 
TGGGATTGGA CGCAGGTCCA TAAGACCTAC TCTCGCCTGC AACAACGTCT GAATCCCTCC 
TATCTTCAAC CGAATAAGCT AAACTTGAAG TTGGCCAACC TAATATACTC CGGTTCCGCA 
GAACTGGGTG TACCACGGAT GAAACAGCCA TTGATAGCGG GTGCTACGTT CGGTTACACT 
CATCATGTCC CGGTGACCGT TAACCAAAGA CGAAGGGCCA GCAGCGCACG TTTGTATTTG 
GCCAATGATC AAGTGAATAG AAGAGGTAAC CTAAAGGTAA TACGAGGAGC TCAAGTTCAG 
CGGGTACTAC TAAATGCGGC TGGATCCCGA GCAACTGGAG TGATTTATAC CCTAAATGGT 
GTGGAACACA CGGCGAAAAC GTTAGGCGAA GTCATCCTAA GCGCAGGAAC CCTCAATTCC 
GCCAAGTTGC TTCTGCTTTC GGGCATCGGG CCGCGGGAGG AGCTACAAAG ATGGAATATA 
ACTACGCACC AGGACCTTCC AGTGGGTCGA AATCTACAAG ATCATGGAAT GATGCCATTG 
TTTCTGCTCT TCGGTTCGAA TTGTGCCGTC AACAGTACAC GGGATCCCAC AGAAAATCCC 
TATGCTCCTG TATCTATCAC ACAGTATCTA CTGGATAATC AGAAGGGTCC TTTGGCCAGT 
GGCTTCTACA TGATGGGCTA CATCAATTCC AGTTCGCCGA GCAGCAGTCG TGGTGAACCC 
GATCTGCATG TGGTGGCCCA CACTCTTTTG CCCAAAGGAA GTACCGGAAG CTTTGGCTAT 
TTGGGCTTTC GGCCAGAGTT GATTCAGGCT CAGCAGGATA TCTTGCAGAA AGGAGATCTA 
CTGCAAATAA TGGGCTCACT GTTGCGTCCC CTTTCACATG GCAAAGTGAG TCTATCGAGT 
AAAAATTCCG CTGATCAGGC AAAAATCGAA AACCACTATG GCGAGGCTGT AGAGGATCAG 
CAGACGCTAC TTCGCTATGT GCGATATATT CAAAAGTTGA GCAAAACCCG ACCTTTTCGT 
CGATGTGGTC TACGACTTTG GAAGCCGCCC CTCCACGAAT GCGATACATT AGCTGCCGAT 
TCCGATGACT ACTGGTTGTG TTACATCCGA TATTTCTATG TGGGTGCCTG GCATTCCGTG 
GGCACTTGTC GCATGGCTCC AAGAAAGGGA GTGGATTCCC AGGAGAATGG AGGCGTGGTG 
GACGAAAGAT TGAGGGTTCA TGGGGTCAAG GGTCTTCGTG TGGTGGATGC CAGCATTATG 
CCGGAATTGC CAGCGGGCAA TACGAATGGA CCCGCCATGA TGATTGGTGA AAAAGGGGCA 
CAAATGATTC TCGACGATCG GGAGGCGAAT AATGAGGTGA TTCAGGAATG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572986.1
CDS	57..2030
Translation	MGDQRVHKPRTAKTRTLRVNRILLPMLLLFSGETTSVVTQLFSVDSSGLGISLMQSVAIALNASSLALANNTAWPLQHEPPEDRLEIESYDYIVVGAGSAGSIVASRLSELCQVKVLLLEEGQLPPLESEIFGLTGALHHDERYMFLEEAVPNPKCCQAMASMHGCVWWHGRMMGGGGAINGNIFIPGSRENFRRWNSTGWDWTQVHKTYSRLQQRLNPSYLQPNKLNLKLANLIYSGSAELGVPRMKQPLIAGATFGYTHHVPVTVNQRRRASSARLYLANDQVNRRGNLKVIRGAQVQRVLLNAAGSRATGVIYTLNGVEHTAKTLGEVILSAGTLNSAKLLLLSGIGPREELQRWNITTHQDLPVGRNLQDHGMMPLFLLFGSNCAVNSTRDPTENPYAPVSITQYLLDNQKGPLASGFYMMGYINSSSPSSSRGEPDLHVVAHTLLPKGSTGSFGYLGFRPELIQAQQDILQKGDLLQIMGSLLRPLSHGKVSLSSKNSADQAKIENHYGEAVEDQQTLLRYVRYIQKLSKTRPFRRCGLRLWKPPLHECDTLAADSDDYWLCYIRYFYVGAWHSVGTCRMAPRKGVDSQENGGVVDERLRVHGVKGLRVVDASIMPELPAGNTNGPAMMIGEKGAQMILDDREANNEVIQEC

Target ORF information:

RefSeq Version	NM_132758.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ecdysone oxidase (Eo), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132758.2

ORF Insert Sequence:

ATGGGCGATC AGCGCGTACA CAAACCGCGG ACAGCGAAGA CAAGAACCCT TCGCGTCAAT 
AGGATATTAT TACCGATGTT GCTTCTGTTT TCAGGAGAAA CTACATCAGT TGTGACCCAA 
TTGTTTTCGG TGGATAGCAG TGGCCTCGGC ATTTCGCTGA TGCAATCGGT GGCCATAGCT 
CTGAATGCAT CCAGCCTCGC CCTGGCCAAC AACACCGCCT GGCCGCTGCA ACACGAACCG 
CCAGAGGATC GGTTGGAGAT CGAGAGCTAT GACTATATAG TGGTGGGTGC AGGTAGTGCC 
GGATCCATTG TGGCCAGTCG ATTGAGTGAG CTGTGCCAGG TGAAGGTCCT GCTGCTGGAG 
GAGGGACAAT TACCACCGCT GGAATCGGAA ATCTTTGGTC TGACCGGTGC ACTGCATCAT 
GACGAGCGGT ACATGTTCCT GGAGGAAGCA GTGCCCAATC CGAAATGCTG CCAGGCAATG 
GCATCCATGC ACGGCTGCGT TTGGTGGCAT GGTCGCATGA TGGGCGGTGG TGGTGCCATT 
AATGGCAATA TCTTTATACC CGGCAGTCGG GAGAACTTCA GACGCTGGAA TTCAACCGGC 
TGGGATTGGA CGCAGGTCCA TAAGACCTAC TCTCGCCTGC AACAACGTCT GAATCCCTCC 
TATCTTCAAC CGAATAAGCT AAACTTGAAG TTGGCCAACC TAATATACTC CGGTTCCGCA 
GAACTGGGTG TACCACGGAT GAAACAGCCA TTGATAGCGG GTGCTACGTT CGGTTACACT 
CATCATGTCC CGGTGACCGT TAACCAAAGA CGAAGGGCCA GCAGCGCACG TTTGTATTTG 
GCCAATGATC AAGTGAATAG AAGAGGTAAC CTAAAGGTAA TACGAGGAGC TCAAGTTCAG 
CGGGTACTAC TAAATGCGGC TGGATCCCGA GCAACTGGAG TGATTTATAC CCTAAATGGT 
GTGGAACACA CGGCGAAAAC GTTAGGCGAA GTCATCCTAA GCGCAGGAAC CCTCAATTCC 
GCCAAGTTGC TTCTGCTTTC GGGCATCGGG CCGCGGGAGG AGCTACAAAG ATGGAATATA 
ACTACGCACC AGGACCTTCC AGTGGGTCGA AATCTACAAG ATCATGGAAT GATGCCATTG 
TTTCTGCTCT TCGGTTCGAA TTGTGCCGTC AACAGTACAC GGGATCCCAC AGAAAATCCC 
TATGCTCCTG TATCTATCAC ACAGTATCTA CTGGATAATC AGAAGGGTCC TTTGGCCAGT 
GGCTTCTACA TGATGGGCTA CATCAATTCC AGTTCGCCGA GCAGCAGTCG TGGTGAACCC 
GATCTGCATG TGGTGGCCCA CACTCTTTTG CCCAAAGGAA GTACCGGAAG CTTTGGCTAT 
TTGGGCTTTC GGCCAGAGTT GATTCAGGCT CAGCAGGATA TCTTGCAGAA AGGAGATCTA 
CTGCAAATAA TGGGCTCACT GTTGCGTCCC CTTTCACATG GCAAAGTGAG TCTATCGAGT 
AAAAATTCCG CTGATCAGGC AAAAATCGAA AACCACTATG GCGAGGCTGT AGAGGATCAG 
CAGACGCTAC TTCGCTATGT GCGATATATT CAAAAGTTGA GCAAAACCCG ACCTTTTCGT 
CGATGTGGTC TACGACTTTG GAAGCCGCCC CTCCACGAAT GCGATACATT AGCTGCCGAT 
TCCGATGACT ACTGGTTGTG TTACATCCGA TATTTCTATG TGGGTGCCTG GCATTCCGTG 
GGCACTTGTC GCATGGCTCC AAGAAAGGGA GTGGATTCCC AGGAGAATGG AGGCGTGGTG 
GACGAAAGAT TGAGGGTTCA TGGGGTCAAG GGTCTTCGTG TGGTGGATGC CAGCATTATG 
CCGGAATTGC CAGCGGGCAA TACGAATGGA CCCGCCATGA TGATTGGTGA AAAAGGGGCA 
CAAATGATTC TCGACGATCG GGAGGCGAAT AATGAGGTGA TTCAGGAATG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Eo cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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