rut cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	rut
Entrez Gene ID	32406
Full Name	rutabaga
Synonyms	AC,Ac12F,CG9533,Dmel\CG9533,EP1603,RUT,Rut,Rut-AC
General protein information	Preferred Names rutabaga Names CG9533 gene product from transcript CG9533-RB CG9533-PA CG9533-PB CG9533-PC CG9533-PD CG9533-PE CG9533-PF CG9533-PG RUTABAGA adenylyl cyclase rut-PA rut-PB rut-PC rut-PD rut-PE rut-PF rut-PG rutabage
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 12F4-12F5

mRNA and Protein(s)

mRNA	Protein	Name
NM_001298317.1	NP_001285246.1	rutabaga, isoform G
NM_001258743.3	NP_001245672.1	rutabaga, isoform B
NM_001272620.2	NP_001259549.1	rutabaga, isoform D
NM_078601.4	NP_511156.2	rutabaga, isoform A
NM_001272619.2	NP_001259548.1	rutabaga, isoform C
NM_001272621.2	NP_001259550.1	rutabaga, isoform E
NM_001272622.2	NP_001259551.1	rutabaga, isoform F

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ADCY1	XP_519081.3
	ADCY1	XP_001086268.1
Mus musculus (house mouse)	Adcy1	NP_033752.1
Gallus gallus (chicken)	ADCY1	XP_418883.4
Xenopus tropicalis (tropical clawed frog)	adcy1	XP_002933461.1
Danio rerio (zebrafish)	adcy1a	NP_001161821.1
Homo sapiens (human)	ADCY1	NP_066939.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000727	XP_559367.4
Canis lupus familiaris (dog)	ADCY1	XP_849081.3
Bos taurus (cattle)	ADCY1	NP_776654.1
Rattus norvegicus (Norway rat)	Adcy1	NP_001100709.1
Drosophila melanogaster (fruit fly)	rut	NP_511156.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mutations in chico reduce expression of the rutabaga-type adenylyl cyclase (rut), leading to decreased cAMP synthesis in the mushroom bodies.
Title: Learning defects in Drosophila growth restricted chico mutants are caused by attenuated adenylyl cyclase activity.

Analyzing acquisition dynamics in Drosophila memory mutants reveals that rutabaga (rut)-dependent cyclic AMP signals couple in a divergent fashion for support of different memory components.
Title: AKAPS act in a two-step mechanism of memory acquisition.

The results of this study supported the idea that GABA-rutabaga-dependent inhibition of gustatory interneurons is critical for PER habituation.
Title: Gustatory habituation in Drosophila relies on rutabaga (adenylate cyclase)-dependent plasticity of GABAergic inhibitory neurons.

Rutabaga-dependent ethanol self-administration may function through alcohol's positive reinforcement of feeding behavior in Drosophila.
Title: The propensity for consuming ethanol in Drosophila requires rutabaga adenylyl cyclase expression within mushroom body neurons.

Our results suggest that Rutabaga acts as a coincidence detector and demonstrate the existence of subcellular domains of PKA activity that could underlie the functional specialization of mushroom body lobes in aversive and appetitive learning.
Title: PKA dynamics in a Drosophila learning center: coincidence detection by rutabaga adenylyl cyclase and spatial regulation by dunce phosphodiesterase.

The following rut gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rut cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01607	NM_078601.4 Latest version!	Drosophila melanogaster rutabaga (rut), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OFa03564	NM_001258743.3 Latest version!	Drosophila melanogaster rutabaga (rut), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OFa03565	NM_001272619.2 Latest version!	Drosophila melanogaster rutabaga (rut), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OFa03566	NM_001272621.2 Latest version!	Drosophila melanogaster rutabaga (rut), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OFa03567	NM_001272622.2 Latest version!	Drosophila melanogaster rutabaga (rut), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OFa03568	NM_001298317.1 Latest version!	Drosophila melanogaster rutabaga (rut), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OFa03568	NM_001272620.2 Latest version!	Drosophila melanogaster rutabaga (rut), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rut ( NM_078601.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_511156.2 Drosophila melanogaster rutabaga (rut), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa01607
Clone ID Related Accession (Same CDS sequence)	NM_078601.4
Accession Version	NM_078601.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	rutabaga, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_078601.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATCATG CCGTGAAGGC GACGCGCGGC CGCCCGTTGA ATACGCTGCG CTTCGAGAAC 
GATGAGCTGG AATGCCTCTA CCAGCGGTAT ACGCTCAAGC TGCAGCGCTT CTCGGTGCTG 
GGCGTGGTGG CTCTGGTCTT CGTCCTCTGC GGCGTGATGG CGGCCCTTTC GCTGACCTTC 
AACAACGCGG CCACCTTTCA CAACATCTTC AATGCGATCG TTTGTGGACT GTTTGCCGTG 
GTGCTGGTGC TGCTGCAGTG TTCCGTGATC AAGGACCACC ACCTGCCCAC ATTGTGCTAC 
GGAATCCTGC TATTCACGGC CAGCATCTGT GTGGTGTCGA TGCCCACATT GGGCAGCGTG 
TTCCCGGTGG ACACCAAGGA GGTGATGGCC GAGGGCGTCT GGCAGATCGT GTTCGTGGTC 
TTCCTCGCTT ACGCCATGAT GCCGCTGCAG ATTTGGGAGG CGGTGGCCTT TGGCATCGCC 
CTGCCCTCGG TGCACATAAG TCTAACGGTC TATAAGATTT TCACGGATGC GCTGCGCTAC 
CTGGAGTATA ATCAACTGAT TGCGAACATT GTCATCTTTA TTGGTGTAAA TGTGGCCGGT 
CTCGTGGTGA ACATCATGAT GGAGCGCGCA CAGCGTCGCA CATTTCTCGA TACACGCAAC 
TGCATCGCAT CCCGTCTGGA GATCCAGGAT GAGAACGAGA AACTGGAACG TCTGTTGCTA 
TCGGTGCTGC CGCAGCACGT GGCCATGCAG ATGAAGAACG ACATCCTGTC GCCGGTGGCT 
GGGCAATTTC ATCGCATCTA CATTCAGAAG CACGAGAATG TGAGCATCCT TTTCGCGGAC 
ATTGTCGGAT TCACGGTGCT CTCCTCGCAG TGTAGTGCCC AGGAGCTGGT CCGCCTGCTC 
AACGAGCTCT TTGGCCGCTT CGATCAGCTG GCACACGATA ATCACTGTTT GCGTATCAAG 
ATCCTGGGCG ACTGTTACTA TTGTGTCTCC GGATTGCCAG AACCTCGCAA GGATCACGCC 
AAATGCGCCG TGGAAATGGG ACTCGATATG ATAGATGCCA TTGCCACCGT GGTGGAGGCC 
ACCGACGTCA TCCTGAACAT GCGCGTGGGC ATCCACACGG GCCGTGTGCT CTGCGGGGTC 
TTGGGCCTTC GCAAGTGGCA GTTCGACGTG TGGTCAAACG ATGTGACCCT GGCCAATCAC 
ATGGAGTCCG GCGGGGAACC GGGTCGGGTG CACGTAACCC GCGCCACCCT GGACTCCTTG 
TCCGGCGAAT ACGAGGTGGA GGCTGGCCAC GGCGACGAAC GGTCCTCGTA TCTGCGCGAC 
CATGGTGTGG ACACGTTCTT CATCGTTCCG CCGCCACATC GACGCAAGCC ACTGATGCTC 
AACACACTGG GCGTTCGCTC GGCGATCGGC AGTCGACGGA AGTTGTCGTT CAGAAACGTG 
TCCAATGTGG TCATGCAGCT GCTGCACACG ATCAAGTTCT CGGAACCGGT GCCGTTCTCC 
AATATAGCCA CCGGATCGTT CCCCTCAGCG GCCAGCGCCT TGGGTGGCGG AGTTTCAGTC 
GGCGGTGGTG GAGGAGGAGG AGGAGGAGGC GTGGCACGGG GTAGCACCTG CGAGGCGAAC 
TCTGGAAACG TTCAGGTGTC GGAGAAGGGA TCGCGCAAGG TGATACGTCT GCAAAAGATC 
TTGCATGCAA CACCGCCACC GCACGGCATG GGCTATGGCA GCGTCGTCGG CAGCGGCGGC 
GGTGTGGACA GCGGTATTTC CGGTGGTGGT GGCTGTGTTT CCGGTGGTAT TGCCGGTGGG 
GGCGGTGTTC AAGTGACCGT AGGCACAAAT CCCAACTCTA CAGCGAGTAC GATTAGTCGC 
ATTCATAGGC ACAATCACAA AAACAATAAA TCCCAGTCGA AGGTGGCGGA CAAATTCAAG 
AGGCCATTCC GGAAGCGGCA CTCGGTGGCC GCCCACCATC AGCCAACGAA TCGGGTGAAT 
CGATTCCTTT CCCAGGCGAT CAATGCCCGG TCGGTGGACT GCGACAAATC GGAGCATGTG 
GATCGATTGA CCCTGCGATT CCGACAGAGC GACATGGAAC GCGAGTATCA CAAGGATTTC 
GATCTGGGCT TCACTACGGC CATGGGTTGC TCCCTGCTGT TGCTCATCCT CGGTGCAGCG 
CTCCAGGTGA CTGCTCTGCC GCGCACTCTC ATCCTGCTGC TGCTCTTTCT CTTTGCCTTC 
ATTTGGGTGA GCGCCATTCT GATGCTCCTG CTCGCCGTGC GTCTCAAATG GATCATCTGG 
GATATTTCGG AGAGCTTTTC GCTGCGCATG GCCATCACGA TTTTCACGGT CATACTCATC 
TATTCCGTGG GACAAGTGAA TGTGTTTACG TGCGTTTCGG ATCATCCATG CTCGGGCAAT 
GGGACGACCT CCTTCCAGAA CGACTCTCAT CGCAAGTGCT CGCTGCCGCA GTACGTGTCC 
CTATCGGCTG CCTTTGCCTT TCTATCTGTA TCGGTATTCC TGAGGCTGCC CATCATATTC 
AAATCGCTCC TAGTTCTGGG CATGGGCACC ATCTATGGCC TTTTCATAGA GCTATCGCAT 
CAGAATATCT TTGAGTGCTA CGACAACCGG GTCAACGCCT CGATTCCACT TCACTTGATC 
TCGCTGGCCC GCATTGCCAT CTTCATGATT GCCATTTTGG TGCATGGACG CCTGGTGGAG 
GGCACTGCCC GGCTGGACTT CCTCTGGCAG CTGCAGGCGT CGCAGGAGAA GAAGGAGATG 
GATGTGCTAC AGGAGTCCAA TAAGCGCATT CTACACAATT TGCTGCCCGC TCATGTGGCT 
GCACATTTTC TCGATGCGCA ATTTCGCAAC AACATGGAAC TCTACCACCA GAGCTATGCC 
AAAGTGGGCG TCATCTTTGC AAGCGTACCG AACTTCAACG AGTTTTACAC GGAAATGGAT 
GGCTCCGATC AGGGTCTTGA GTGCCTCCGT CTGCTCAACG AGATTATTGC GGACTTTGAT 
GAGCTGCTCA AGGAGGATCG TTTCCGCGGC ATCGACAAGA TCAAGACCGT TGGTAGCACT 
TATATGGCTG TCGTGGGACT CATACCCGAG TACAAAATCC AGCCTAACGA TCCGAACTCA 
GTGCGGCGCC ACATGACAGC GCTCATTGAG TATGTCAAGG CCATGAGGCA CTCACTCCAA 
GAGATAAACA GTCACTCGTA CAATAACTTC ATGCTGCGCG TGGGCATCAA CATTGGACCC 
GTTGTGGCGG GCGTAATTGG AGCTCGAAAG CCACAATATG ACATTTGGGG CAATACGGTG 
AACGTGGCCA GTCGCATGGA CTCCACCGGA GTTCCCGGCT ACTCGCAAGT AACTCAAGAG 
GTGGTCGACA GCCTGGTGGG GTCGCACTTT GAATTCCGCT GTCGTGGCAC CATTAAGGTG 
AAGGGCAAAG GCGACATGGT CACATATTTC TTGTGTGACA GCGGCAACAA ATCGCTGAAC 
GGAGAAGTGC GCAACGCCAT GTCCCTGCCA CAGAGTCTCC ATGCGCCGGA TTACTATATG 
AAGGTGTCGC AGTTTCCGGA GAATCGCGTC AATACCGACA CCTATAGCAA AAAGGAGAAT 
GGACATCTGT ACGCTGGCAA CGGCGTCGAG GAGCAGCAGT TGTTGCTGCA GCATCAGCAC 
AAACAGCACG ATCCGCTACC GCTTCCTGCT CCTCCGCCTC CGGTTCACCA TCATTTGCAT 
CAGCAGCAAC AGCAGCGTTT GAACAGCAAG CTGCAAAAGC AACCGATCTT CATGGCCAAC 
GGTGGTCTGC CCAATATCCG GGAGAATGGC AATGGTCACA ATGGAGAGCA TCAGCAGCAG 
CAGCAGCAGC AGCAGCAACA TCAGCAGCAG CAACAGCAGC AGCAGCAGCA CGGTGGCTTT 
ATGGTGGCAA CGACGACACC GCCGGCTGCA GTGGCGGTTC CCCTCCAGCC CCAGCACCAT 
CAACTCCAGT TCCAGCACCC GCACCAGCAT CCACTCCCGT CAGCAGTGTC GGTTCCAGTG 
CAGCACCAGA TCCTACTCCA CCACCAGCTC CAGCTCCAGC ACCAGCCCGT TCCTTCGGTA 
ATGTTGCGCG AGTTTAATAT TATTGAGAAT CCGACTTCCG GTGGCCGGCA TCAGCAGATG 
GAGCAGCTGC CGCCGCATCA CGGCAGCCTC GACCTATCGG GAATGGGAAT GGGTGTGGGG 
GCTGGCGTGC TCGGCAGCGA CTGCTTCATG ATGCCACGGC GAGATCGCGA ACGCACATAT 
GTGCCGCCCT TAAACCAGCA TGGCCATCAT CCACCGCATC ACCTCCATTC GAATCTGAAT 
CTGAATCAGA GCCAGCACCC GCCATCGTTC ACCAGCCTGG GCTATGGCCA ATGCCGGGAG 
AGCGAGCCGC TATTGCATGC CAGCAGCGTC GCGCCGGTAG CCAAGATCAT GCCCATGCAG 
CATGCGCCAA AGTACGAGCC ACCACGTTAC ACTTCTCCCC ACACCATGTT GTCGCAACAG 
CACCAGCAGC AGCAGCAGCA GCAGCATCAG CATCAGCAGC CGCAATCGCA ATCTGCCCAG 
GATCAGCAGA CACATCCCGC CCAGGACCCA CATCCTTTGC AGCGGCAGTA CGCCATGTAT 
TCGCAGCAGC CGCAGCTGCC GCCGAAGCCA GTGCTGAGAA CGTACATGAA GCCGTTGCCG 
AAGCTGCCGA CGGATCTGGA GGAGAGCCGC GACATGTCCT CGACGGATGA TCTGAGCTCA 
CGACCACACT CGCCCTCGAT GAGCAGCTCG GATGAGAGCT ACTCGAAGAC CACAGAGGGC 
GAAGGTGAAG GCGATGAGGA CTCGCCGCGC ATGGTCAATG GTGGCCACCT GCATCATCGC 
AATGGCTACC ACCTGCCCGC CGGTGGTCTG GTCAACCCAT TGCAATGGCT CTACCCCTGC 
GACATTCAGG TGGATCCCAC CTCTCCGGTC GTCGACATGG CCCACCTGCA TGACTTTGAG 
CTAAGCTCCA CCACCGAGAG CCAGGGCCAT CACACTAACA GCAACACGAC GAGCAACACA 
CAACACAAGG GCGACAGCTG CAACAGCTTC GACTTTCAGA AGGCAGCAGT TGGAACGGCA 
GCGGGTGCGG CAATCGCGAC CAAGTCGCCC TTCGAGCGTG AACTGCAACG TCTGCTTAAC 
GAATCCTCGC GAGCTCGTTG CCTGGCGACT GCGACAACGA CGGCTGGAGC CATCTCTACC 
ACAGATCAGA CGGCCAGCAA TGGAAGCCGT GAGTTGAGCT ACTCCCTCAG CAATGGGAAG 
CTGAGCTCTG CGAATGGACA TGGCGTTGGA GGATCCGGAT CCGGATCCGG ATCGGGATCA 
GGTTCAGGTT CGGCGGTGGG CAATGGCAGC GGTGGCAGCG GCAGCAGCAA TGGCAATCTC 
AGCGGCGGCA GCGGCAGCAA CTCGAACAGT GGCAACAACA ACAGCAGTCA CCACAAGACG 
GAGCAGCAGC AGAATATGGA TCACGAGCAT CTGGCCGGTG GCAAATTGCT GGGCAGTAAT 
AGCTTCATGA TCGCCAAACA TCCGGTGGGC TTGGAAGCCA TCAAGGAGAT AACGCGCAAC 
AAGAATCCCT CGGAGTCCAG CCAAATGCAA ACCTCGGACA CGGAGTCCTG CGAGATTCTG 
CACGAGAACC GCAACCAGAT GCATGTGCTG GCCATGCTGG AGATGCATAC GGCCAAGGAG 
CTGAATGGCA GCCATGCGCA TCACGGTCAG CATCACCAGC AGCCACAGAG GACGCATCGC 
CAGCGACCGC GCTCCAAGGA GCTGCAGTAC AGCCACGAGA GTCTGGACGG ACTGGATGGA 
GCGGTGCAGT CGCAGTCGCA GCAGCGACAC CAACGCTATC ATCACCACCA TCACCACCAA 
CAGAGGCAGC AGCAACAGCA GCGCTACAAC CATGTGCAGG AGCAGGAGGA GCGTGACGAC 
ACCGAGGACA ACCTGGCCGA TGAGGAGTTT GAGGACGACG AAGTGGGTCG TGATGTGCGA 
CAGAAGCGCC TACAAAAGTC CGAGCTCAAT CACAAACGAT CGGAGGTTGC AACAGAGGCA 
GGCAATCACC ACGACGACGA GGTGGAGGAG GAGGACGACG ATGACGACGA AGAGGAGGAC 
CACCGGAATG GCGGGCGGGA GGCGGCACCG CTGACTAACG GCAGCATGCG CGGCCTGGAG 
GCCAATGTGA TAAACGACGA GCTGAAGTAT GGAGCAACCC ATCTCAATCA CCAGTCCATG 
GACTCCAATC CGTTGGAGAG CCAATCGGAG TGGTCGGATG ACGATTGTCG TGAGGAAGCA 
ACAGGGGGAG CCGAGTCCAC GGGCTACATT ACGGATGAGC CCGGACTGGA GAACATCTCG 
TTGCTGAACG AAGCTGGTCT CACCGATGCC GAGGGCGCTC TCTCGGATGT CAACTCGCTG 
TATAACGCGC CGGACGTGGA CGACACTTCG GTGTCCAGCC GGGCCAGCTC CCGCCTGCTT 
AGCCTGGACT CGCTGAGCGG CCTCTACGAC TGCGATCTGG ACTCCAAGCA CGAGCTGGCC 
ATCGTCAATG CCTCGCACAA GATCTCCAGT AAATTTGGGC AGCCTCTATC ACCGGCACAG 
CAGCAACATC AACAACAGCA GCAACAGCAA CAGCAGCAAC AGCAGCAACA CCACCAACAG 
CAGCTGCAGC AGAATCCGCA GCACACTCAA GCCCAATCGC ATTTGGCTCC GGTTCAGTTT 
CAGTCCGCCG AGGAGTTGAG AGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_511156.2
CDS	331..7077
Translation	MDHAVKATRGRPLNTLRFENDELECLYQRYTLKLQRFSVLGVVALVFVLCGVMAALSLTFNNAATFHNIFNAIVCGLFAVVLVLLQCSVIKDHHLPTLCYGILLFTASICVVSMPTLGSVFPVDTKEVMAEGVWQIVFVVFLAYAMMPLQIWEAVAFGIALPSVHISLTVYKIFTDALRYLEYNQLIANIVIFIGVNVAGLVVNIMMERAQRRTFLDTRNCIASRLEIQDENEKLERLLLSVLPQHVAMQMKNDILSPVAGQFHRIYIQKHENVSILFADIVGFTVLSSQCSAQELVRLLNELFGRFDQLAHDNHCLRIKILGDCYYCVSGLPEPRKDHAKCAVEMGLDMIDAIATVVEATDVILNMRVGIHTGRVLCGVLGLRKWQFDVWSNDVTLANHMESGGEPGRVHVTRATLDSLSGEYEVEAGHGDERSSYLRDHGVDTFFIVPPPHRRKPLMLNTLGVRSAIGSRRKLSFRNVSNVVMQLLHTIKFSEPVPFSNIATGSFPSAASALGGGVSVGGGGGGGGGGVARGSTCEANSGNVQVSEKGSRKVIRLQKILHATPPPHGMGYGSVVGSGGGVDSGISGGGGCVSGGIAGGGGVQVTVGTNPNSTASTISRIHRHNHKNNKSQSKVADKFKRPFRKRHSVAAHHQPTNRVNRFLSQAINARSVDCDKSEHVDRLTLRFRQSDMEREYHKDFDLGFTTAMGCSLLLLILGAALQVTALPRTLILLLLFLFAFIWVSAILMLLLAVRLKWIIWDISESFSLRMAITIFTVILIYSVGQVNVFTCVSDHPCSGNGTTSFQNDSHRKCSLPQYVSLSAAFAFLSVSVFLRLPIIFKSLLVLGMGTIYGLFIELSHQNIFECYDNRVNASIPLHLISLARIAIFMIAILVHGRLVEGTARLDFLWQLQASQEKKEMDVLQESNKRILHNLLPAHVAAHFLDAQFRNNMELYHQSYAKVGVIFASVPNFNEFYTEMDGSDQGLECLRLLNEIIADFDELLKEDRFRGIDKIKTVGSTYMAVVGLIPEYKIQPNDPNSVRRHMTALIEYVKAMRHSLQEINSHSYNNFMLRVGINIGPVVAGVIGARKPQYDIWGNTVNVASRMDSTGVPGYSQVTQEVVDSLVGSHFEFRCRGTIKVKGKGDMVTYFLCDSGNKSLNGEVRNAMSLPQSLHAPDYYMKVSQFPENRVNTDTYSKKENGHLYAGNGVEEQQLLLQHQHKQHDPLPLPAPPPPVHHHLHQQQQQRLNSKLQKQPIFMANGGLPNIRENGNGHNGEHQQQQQQQQQHQQQQQQQQQHGGFMVATTTPPAAVAVPLQPQHHQLQFQHPHQHPLPSAVSVPVQHQILLHHQLQLQHQPVPSVMLREFNIIENPTSGGRHQQMEQLPPHHGSLDLSGMGMGVGAGVLGSDCFMMPRRDRERTYVPPLNQHGHHPPHHLHSNLNLNQSQHPPSFTSLGYGQCRESEPLLHASSVAPVAKIMPMQHAPKYEPPRYTSPHTMLSQQHQQQQQQQHQHQQPQSQSAQDQQTHPAQDPHPLQRQYAMYSQQPQLPPKPVLRTYMKPLPKLPTDLEESRDMSSTDDLSSRPHSPSMSSSDESYSKTTEGEGEGDEDSPRMVNGGHLHHRNGYHLPAGGLVNPLQWLYPCDIQVDPTSPVVDMAHLHDFELSSTTESQGHHTNSNTTSNTQHKGDSCNSFDFQKAAVGTAAGAAIATKSPFERELQRLLNESSRARCLATATTTAGAISTTDQTASNGSRELSYSLSNGKLSSANGHGVGGSGSGSGSGSGSGSAVGNGSGGSGSSNGNLSGGSGSNSNSGNNNSSHHKTEQQQNMDHEHLAGGKLLGSNSFMIAKHPVGLEAIKEITRNKNPSESSQMQTSDTESCEILHENRNQMHVLAMLEMHTAKELNGSHAHHGQHHQQPQRTHRQRPRSKELQYSHESLDGLDGAVQSQSQQRHQRYHHHHHHQQRQQQQQRYNHVQEQEERDDTEDNLADEEFEDDEVGRDVRQKRLQKSELNHKRSEVATEAGNHHDDEVEEEDDDDDEEEDHRNGGREAAPLTNGSMRGLEANVINDELKYGATHLNHQSMDSNPLESQSEWSDDDCREEATGGAESTGYITDEPGLENISLLNEAGLTDAEGALSDVNSLYNAPDVDDTSVSSRASSRLLSLDSLSGLYDCDLDSKHELAIVNASHKISSKFGQPLSPAQQQHQQQQQQQQQQQQQHHQQQLQQNPQHTQAQSHLAPVQFQSAEELRE

Target ORF information:

RefSeq Version	NM_078601.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster rutabaga (rut), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078601.4

ORF Insert Sequence:

ATGGATCATG CCGTGAAGGC GACGCGCGGC CGCCCGTTGA ATACGCTGCG CTTCGAGAAC 
GATGAGCTGG AATGCCTCTA CCAGCGGTAT ACGCTCAAGC TGCAGCGCTT CTCGGTGCTG 
GGCGTGGTGG CTCTGGTCTT CGTCCTCTGC GGCGTGATGG CGGCCCTTTC GCTGACCTTC 
AACAACGCGG CCACCTTTCA CAACATCTTC AATGCGATCG TTTGTGGACT GTTTGCCGTG 
GTGCTGGTGC TGCTGCAGTG TTCCGTGATC AAGGACCACC ACCTGCCCAC ATTGTGCTAC 
GGAATCCTGC TATTCACGGC CAGCATCTGT GTGGTGTCGA TGCCCACATT GGGCAGCGTG 
TTCCCGGTGG ACACCAAGGA GGTGATGGCC GAGGGCGTCT GGCAGATCGT GTTCGTGGTC 
TTCCTCGCTT ACGCCATGAT GCCGCTGCAG ATTTGGGAGG CGGTGGCCTT TGGCATCGCC 
CTGCCCTCGG TGCACATAAG TCTAACGGTC TATAAGATTT TCACGGATGC GCTGCGCTAC 
CTGGAGTATA ATCAACTGAT TGCGAACATT GTCATCTTTA TTGGTGTAAA TGTGGCCGGT 
CTCGTGGTGA ACATCATGAT GGAGCGCGCA CAGCGTCGCA CATTTCTCGA TACACGCAAC 
TGCATCGCAT CCCGTCTGGA GATCCAGGAT GAGAACGAGA AACTGGAACG TCTGTTGCTA 
TCGGTGCTGC CGCAGCACGT GGCCATGCAG ATGAAGAACG ACATCCTGTC GCCGGTGGCT 
GGGCAATTTC ATCGCATCTA CATTCAGAAG CACGAGAATG TGAGCATCCT TTTCGCGGAC 
ATTGTCGGAT TCACGGTGCT CTCCTCGCAG TGTAGTGCCC AGGAGCTGGT CCGCCTGCTC 
AACGAGCTCT TTGGCCGCTT CGATCAGCTG GCACACGATA ATCACTGTTT GCGTATCAAG 
ATCCTGGGCG ACTGTTACTA TTGTGTCTCC GGATTGCCAG AACCTCGCAA GGATCACGCC 
AAATGCGCCG TGGAAATGGG ACTCGATATG ATAGATGCCA TTGCCACCGT GGTGGAGGCC 
ACCGACGTCA TCCTGAACAT GCGCGTGGGC ATCCACACGG GCCGTGTGCT CTGCGGGGTC 
TTGGGCCTTC GCAAGTGGCA GTTCGACGTG TGGTCAAACG ATGTGACCCT GGCCAATCAC 
ATGGAGTCCG GCGGGGAACC GGGTCGGGTG CACGTAACCC GCGCCACCCT GGACTCCTTG 
TCCGGCGAAT ACGAGGTGGA GGCTGGCCAC GGCGACGAAC GGTCCTCGTA TCTGCGCGAC 
CATGGTGTGG ACACGTTCTT CATCGTTCCG CCGCCACATC GACGCAAGCC ACTGATGCTC 
AACACACTGG GCGTTCGCTC GGCGATCGGC AGTCGACGGA AGTTGTCGTT CAGAAACGTG 
TCCAATGTGG TCATGCAGCT GCTGCACACG ATCAAGTTCT CGGAACCGGT GCCGTTCTCC 
AATATAGCCA CCGGATCGTT CCCCTCAGCG GCCAGCGCCT TGGGTGGCGG AGTTTCAGTC 
GGCGGTGGTG GAGGAGGAGG AGGAGGAGGC GTGGCACGGG GTAGCACCTG CGAGGCGAAC 
TCTGGAAACG TTCAGGTGTC GGAGAAGGGA TCGCGCAAGG TGATACGTCT GCAAAAGATC 
TTGCATGCAA CACCGCCACC GCACGGCATG GGCTATGGCA GCGTCGTCGG CAGCGGCGGC 
GGTGTGGACA GCGGTATTTC CGGTGGTGGT GGCTGTGTTT CCGGTGGTAT TGCCGGTGGG 
GGCGGTGTTC AAGTGACCGT AGGCACAAAT CCCAACTCTA CAGCGAGTAC GATTAGTCGC 
ATTCATAGGC ACAATCACAA AAACAATAAA TCCCAGTCGA AGGTGGCGGA CAAATTCAAG 
AGGCCATTCC GGAAGCGGCA CTCGGTGGCC GCCCACCATC AGCCAACGAA TCGGGTGAAT 
CGATTCCTTT CCCAGGCGAT CAATGCCCGG TCGGTGGACT GCGACAAATC GGAGCATGTG 
GATCGATTGA CCCTGCGATT CCGACAGAGC GACATGGAAC GCGAGTATCA CAAGGATTTC 
GATCTGGGCT TCACTACGGC CATGGGTTGC TCCCTGCTGT TGCTCATCCT CGGTGCAGCG 
CTCCAGGTGA CTGCTCTGCC GCGCACTCTC ATCCTGCTGC TGCTCTTTCT CTTTGCCTTC 
ATTTGGGTGA GCGCCATTCT GATGCTCCTG CTCGCCGTGC GTCTCAAATG GATCATCTGG 
GATATTTCGG AGAGCTTTTC GCTGCGCATG GCCATCACGA TTTTCACGGT CATACTCATC 
TATTCCGTGG GACAAGTGAA TGTGTTTACG TGCGTTTCGG ATCATCCATG CTCGGGCAAT 
GGGACGACCT CCTTCCAGAA CGACTCTCAT CGCAAGTGCT CGCTGCCGCA GTACGTGTCC 
CTATCGGCTG CCTTTGCCTT TCTATCTGTA TCGGTATTCC TGAGGCTGCC CATCATATTC 
AAATCGCTCC TAGTTCTGGG CATGGGCACC ATCTATGGCC TTTTCATAGA GCTATCGCAT 
CAGAATATCT TTGAGTGCTA CGACAACCGG GTCAACGCCT CGATTCCACT TCACTTGATC 
TCGCTGGCCC GCATTGCCAT CTTCATGATT GCCATTTTGG TGCATGGACG CCTGGTGGAG 
GGCACTGCCC GGCTGGACTT CCTCTGGCAG CTGCAGGCGT CGCAGGAGAA GAAGGAGATG 
GATGTGCTAC AGGAGTCCAA TAAGCGCATT CTACACAATT TGCTGCCCGC TCATGTGGCT 
GCACATTTTC TCGATGCGCA ATTTCGCAAC AACATGGAAC TCTACCACCA GAGCTATGCC 
AAAGTGGGCG TCATCTTTGC AAGCGTACCG AACTTCAACG AGTTTTACAC GGAAATGGAT 
GGCTCCGATC AGGGTCTTGA GTGCCTCCGT CTGCTCAACG AGATTATTGC GGACTTTGAT 
GAGCTGCTCA AGGAGGATCG TTTCCGCGGC ATCGACAAGA TCAAGACCGT TGGTAGCACT 
TATATGGCTG TCGTGGGACT CATACCCGAG TACAAAATCC AGCCTAACGA TCCGAACTCA 
GTGCGGCGCC ACATGACAGC GCTCATTGAG TATGTCAAGG CCATGAGGCA CTCACTCCAA 
GAGATAAACA GTCACTCGTA CAATAACTTC ATGCTGCGCG TGGGCATCAA CATTGGACCC 
GTTGTGGCGG GCGTAATTGG AGCTCGAAAG CCACAATATG ACATTTGGGG CAATACGGTG 
AACGTGGCCA GTCGCATGGA CTCCACCGGA GTTCCCGGCT ACTCGCAAGT AACTCAAGAG 
GTGGTCGACA GCCTGGTGGG GTCGCACTTT GAATTCCGCT GTCGTGGCAC CATTAAGGTG 
AAGGGCAAAG GCGACATGGT CACATATTTC TTGTGTGACA GCGGCAACAA ATCGCTGAAC 
GGAGAAGTGC GCAACGCCAT GTCCCTGCCA CAGAGTCTCC ATGCGCCGGA TTACTATATG 
AAGGTGTCGC AGTTTCCGGA GAATCGCGTC AATACCGACA CCTATAGCAA AAAGGAGAAT 
GGACATCTGT ACGCTGGCAA CGGCGTCGAG GAGCAGCAGT TGTTGCTGCA GCATCAGCAC 
AAACAGCACG ATCCGCTACC GCTTCCTGCT CCTCCGCCTC CGGTTCACCA TCATTTGCAT 
CAGCAGCAAC AGCAGCGTTT GAACAGCAAG CTGCAAAAGC AACCGATCTT CATGGCCAAC 
GGTGGTCTGC CCAATATCCG GGAGAATGGC AATGGTCACA ATGGAGAGCA TCAGCAGCAG 
CAGCAGCAGC AGCAGCAACA TCAGCAGCAG CAACAGCAGC AGCAGCAGCA CGGTGGCTTT 
ATGGTGGCAA CGACGACACC GCCGGCTGCA GTGGCGGTTC CCCTCCAGCC CCAGCACCAT 
CAACTCCAGT TCCAGCACCC GCACCAGCAT CCACTCCCGT CAGCAGTGTC GGTTCCAGTG 
CAGCACCAGA TCCTACTCCA CCACCAGCTC CAGCTCCAGC ACCAGCCCGT TCCTTCGGTA 
ATGTTGCGCG AGTTTAATAT TATTGAGAAT CCGACTTCCG GTGGCCGGCA TCAGCAGATG 
GAGCAGCTGC CGCCGCATCA CGGCAGCCTC GACCTATCGG GAATGGGAAT GGGTGTGGGG 
GCTGGCGTGC TCGGCAGCGA CTGCTTCATG ATGCCACGGC GAGATCGCGA ACGCACATAT 
GTGCCGCCCT TAAACCAGCA TGGCCATCAT CCACCGCATC ACCTCCATTC GAATCTGAAT 
CTGAATCAGA GCCAGCACCC GCCATCGTTC ACCAGCCTGG GCTATGGCCA ATGCCGGGAG 
AGCGAGCCGC TATTGCATGC CAGCAGCGTC GCGCCGGTAG CCAAGATCAT GCCCATGCAG 
CATGCGCCAA AGTACGAGCC ACCACGTTAC ACTTCTCCCC ACACCATGTT GTCGCAACAG 
CACCAGCAGC AGCAGCAGCA GCAGCATCAG CATCAGCAGC CGCAATCGCA ATCTGCCCAG 
GATCAGCAGA CACATCCCGC CCAGGACCCA CATCCTTTGC AGCGGCAGTA CGCCATGTAT 
TCGCAGCAGC CGCAGCTGCC GCCGAAGCCA GTGCTGAGAA CGTACATGAA GCCGTTGCCG 
AAGCTGCCGA CGGATCTGGA GGAGAGCCGC GACATGTCCT CGACGGATGA TCTGAGCTCA 
CGACCACACT CGCCCTCGAT GAGCAGCTCG GATGAGAGCT ACTCGAAGAC CACAGAGGGC 
GAAGGTGAAG GCGATGAGGA CTCGCCGCGC ATGGTCAATG GTGGCCACCT GCATCATCGC 
AATGGCTACC ACCTGCCCGC CGGTGGTCTG GTCAACCCAT TGCAATGGCT CTACCCCTGC 
GACATTCAGG TGGATCCCAC CTCTCCGGTC GTCGACATGG CCCACCTGCA TGACTTTGAG 
CTAAGCTCCA CCACCGAGAG CCAGGGCCAT CACACTAACA GCAACACGAC GAGCAACACA 
CAACACAAGG GCGACAGCTG CAACAGCTTC GACTTTCAGA AGGCAGCAGT TGGAACGGCA 
GCGGGTGCGG CAATCGCGAC CAAGTCGCCC TTCGAGCGTG AACTGCAACG TCTGCTTAAC 
GAATCCTCGC GAGCTCGTTG CCTGGCGACT GCGACAACGA CGGCTGGAGC CATCTCTACC 
ACAGATCAGA CGGCCAGCAA TGGAAGCCGT GAGTTGAGCT ACTCCCTCAG CAATGGGAAG 
CTGAGCTCTG CGAATGGACA TGGCGTTGGA GGATCCGGAT CCGGATCCGG ATCGGGATCA 
GGTTCAGGTT CGGCGGTGGG CAATGGCAGC GGTGGCAGCG GCAGCAGCAA TGGCAATCTC 
AGCGGCGGCA GCGGCAGCAA CTCGAACAGT GGCAACAACA ACAGCAGTCA CCACAAGACG 
GAGCAGCAGC AGAATATGGA TCACGAGCAT CTGGCCGGTG GCAAATTGCT GGGCAGTAAT 
AGCTTCATGA TCGCCAAACA TCCGGTGGGC TTGGAAGCCA TCAAGGAGAT AACGCGCAAC 
AAGAATCCCT CGGAGTCCAG CCAAATGCAA ACCTCGGACA CGGAGTCCTG CGAGATTCTG 
CACGAGAACC GCAACCAGAT GCATGTGCTG GCCATGCTGG AGATGCATAC GGCCAAGGAG 
CTGAATGGCA GCCATGCGCA TCACGGTCAG CATCACCAGC AGCCACAGAG GACGCATCGC 
CAGCGACCGC GCTCCAAGGA GCTGCAGTAC AGCCACGAGA GTCTGGACGG ACTGGATGGA 
GCGGTGCAGT CGCAGTCGCA GCAGCGACAC CAACGCTATC ATCACCACCA TCACCACCAA 
CAGAGGCAGC AGCAACAGCA GCGCTACAAC CATGTGCAGG AGCAGGAGGA GCGTGACGAC 
ACCGAGGACA ACCTGGCCGA TGAGGAGTTT GAGGACGACG AAGTGGGTCG TGATGTGCGA 
CAGAAGCGCC TACAAAAGTC CGAGCTCAAT CACAAACGAT CGGAGGTTGC AACAGAGGCA 
GGCAATCACC ACGACGACGA GGTGGAGGAG GAGGACGACG ATGACGACGA AGAGGAGGAC 
CACCGGAATG GCGGGCGGGA GGCGGCACCG CTGACTAACG GCAGCATGCG CGGCCTGGAG 
GCCAATGTGA TAAACGACGA GCTGAAGTAT GGAGCAACCC ATCTCAATCA CCAGTCCATG 
GACTCCAATC CGTTGGAGAG CCAATCGGAG TGGTCGGATG ACGATTGTCG TGAGGAAGCA 
ACAGGGGGAG CCGAGTCCAC GGGCTACATT ACGGATGAGC CCGGACTGGA GAACATCTCG 
TTGCTGAACG AAGCTGGTCT CACCGATGCC GAGGGCGCTC TCTCGGATGT CAACTCGCTG 
TATAACGCGC CGGACGTGGA CGACACTTCG GTGTCCAGCC GGGCCAGCTC CCGCCTGCTT 
AGCCTGGACT CGCTGAGCGG CCTCTACGAC TGCGATCTGG ACTCCAAGCA CGAGCTGGCC 
ATCGTCAATG CCTCGCACAA GATCTCCAGT AAATTTGGGC AGCCTCTATC ACCGGCACAG 
CAGCAACATC AACAACAGCA GCAACAGCAA CAGCAGCAAC AGCAGCAACA CCACCAACAG 
CAGCTGCAGC AGAATCCGCA GCACACTCAA GCCCAATCGC ATTTGGCTCC GGTTCAGTTT 
CAGTCCGCCG AGGAGTTGAG AGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rut ( NM_001258743.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245672.1 Drosophila melanogaster rutabaga (rut), transcript variant B, mRNA.

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CloneID	OFa03564
Clone ID Related Accession (Same CDS sequence)	NM_001258743.3
Accession Version	NM_001258743.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6168bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	rutabaga, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001258743.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATCATG CCGTGAAGGC GACGCGCGGC CGCCCGTTGA ATACGCTGCG CTTCGAGAAC 
GATGAGCTGG AATGCCTCTA CCAGCGGTAT ACGCTCAAGC TGCAGCGCTT CTCGGTGCTG 
GGCGTGGTGG CTCTGGTCTT CGTCCTCTGC GGCGTGATGG CGGCCCTTTC GCTGACCTTC 
AACAACGCGG CCACCTTTCA CAACATCTTC AATGCGATCG TTTGTGGACT GTTTGCCGTG 
GTGCTGGTGC TGCTGCAGTG TTCCGTGATC AAGGACCACC ACCTGCCCAC ATTGTGCTAC 
GGAATCCTGC TATTCACGGC CAGCATCTGT GTGGTGTCGA TGCCCACATT GGGCAGCGTG 
TTCCCGGTGG ACACCAAGGA GGTGATGGCC GAGGGCGTCT GGCAGATCGT GTTCGTGGTC 
TTCCTCGCTT ACGCCATGAT GCCGCTGCAG ATTTGGGAGG CGGTGGCCTT TGGCATCGCC 
CTGCCCTCGG TGCACATAAG TCTAACGGTC TATAAGATTT TCACGGATGC GCTGCGCTAC 
CTGGAGTATA ATCAACTGAT TGCGAACATT GTCATCTTTA TTGGTGTAAA TGTGGCCGGT 
CTCGTGGTGA ACATCATGAT GGAGCGCGCA CAGCGTCGCA CATTTCTCGA TACACGCAAC 
TGCATCGCAT CCCGTCTGGA GATCCAGGAT GAGAACGAGA AACTGGAACG TCTGTTGCTA 
TCGGTGCTGC CGCAGCACGT GGCCATGCAG ATGAAGAACG ACATCCTGTC GCCGGTGGCT 
GGGCAATTTC ATCGCATCTA CATTCAGAAG CACGAGAATG TGAGCATCCT TTTCGCGGAC 
ATTGTCGGAT TCACGGTGCT CTCCTCGCAG TGTAGTGCCC AGGAGCTGGT CCGCCTGCTC 
AACGAGCTCT TTGGCCGCTT CGATCAGCTG GCACACGATA ATCACTGTTT GCGTATCAAG 
ATCCTGGGCG ACTGTTACTA TTGTGTCTCC GGATTGCCAG AACCTCGCAA GGATCACGCC 
AAATGCGCCG TGGAAATGGG ACTCGATATG ATAGATGCCA TTGCCACCGT GGTGGAGGCC 
ACCGACGTCA TCCTGAACAT GCGCGTGGGC ATCCACACGG GCCGTGTGCT CTGCGGGGTC 
TTGGGCCTTC GCAAGTGGCA GTTCGACGTG TGGTCAAACG ATGTGACCCT GGCCAATCAC 
ATGGAGTCCG GCGGGGAACC GGGTCGGGTG CACGTAACCC GCGCCACCCT GGACTCCTTG 
TCCGGCGAAT ACGAGGTGGA GGCTGGCCAC GGCGACGAAC GGTCCTCGTA TCTGCGCGAC 
CATGGTGTGG ACACGTTCTT CATCGTTCCG CCGCCACATC GACGCAAGCC ACTGATGCTC 
AACACACTGG GCGTTCGCTC GGCGATCGGC AGTCGACGGA AGTTGTCGTT CAGAAACGTG 
TCCAATGTGG TCATGCAGCT GCTGCACACG ATCAAGTTCT CGGAACCGGT GCCGTTCTCC 
AATATAGCCA CCGGATCGTT CCCCTCAGCG GCCAGCGCCT TGGGTGGCGG AGTTTCAGTC 
GGCGGTGGTG GAGGAGGAGG AGGAGGAGGC GTGGCACGGG GTAGCACCTG CGAGGCGAAC 
TCTGGAAACG TTCAGGTGTC GGAGAAGGGA TCGCGCAAGT CCCAGTCGAA GGTGGCGGAC 
AAATTCAAGA GGCCATTCCG GAAGCGGCAC TCGGTGGCCG CCCACCATCA GCCAACGAAT 
CGGGTGAATC GATTCCTTTC CCAGGCGATC AATGCCCGGT CGGTGGACTG CGACAAATCG 
GAGCATGTGG ATCGATTGAC CCTGCGATTC CGACAGAGCG ACATGGAACG CGAGTATCAC 
AAGGATTTCG ATCTGGGCTT CACTACGGCC ATGGGTTGCT CCCTGCTGTT GCTCATCCTC 
GGTGCAGCGC TCCAGGTGAC TGCTCTGCCG CGCACTCTCA TCCTGCTGCT GCTCTTTCTC 
TTTGCCTTCA TTTGGGTGAG CGCCATTCTG ATGCTCCTGC TCGCCGTGCG TCTCAAATGG 
ATCATCTGGG ATATTTCGGA GAGCTTTTCG CTGCGCATGG CCATCACGAT TTTCACGGTC 
ATACTCATCT ATTCCGTGGG ACAAGTGAAT GTGTTTACGT GCGTTTCGGA TCATCCATGC 
TCGGGCAATG GGACGACCTC CTTCCAGAAC GACTCTCATC GCAAGTGCTC GCTGCCGCAG 
TACGTGTCCC TATCGGCTGC CTTTGCCTTT CTATCTGTAT CGGTATTCCT GAGGCTGCCC 
ATCATATTCA AATCGCTCCT AGTTCTGGGC ATGGGCACCA TCTATGGCCT TTTCATAGAG 
CTATCGCATC AGAATATCTT TGAGTGCTAC GACAACCGGG TCAACGCCTC GATTCCACTT 
CACTTGATCT CGCTGGCCCG CATTGCCATC TTCATGATTG CCATTTTGGT GCATGGACGC 
CTGGTGGAGG GCACTGCCCG GCTGGACTTC CTCTGGCAGC TGCAGGCGTC GCAGGAGAAG 
AAGGAGATGG ATGTGCTACA GGAGTCCAAT AAGCGCATTC TACACAATTT GCTGCCCGCT 
CATGTGGCTG CACATTTTCT CGATGCGCAA TTTCGCAACA ACATGGAACT CTACCACCAG 
AGCTATGCCA AAGTGGGCGT CATCTTTGCA AGCGTACCGA ACTTCAACGA GTTTTACACG 
GAAATGGATG GCTCCGATCA GGGTCTTGAG TGCCTCCGTC TGCTCAACGA GATTATTGCG 
GACTTTGATG AGCTGCTCAA GGAGGATCGT TTCCGCGGCA TCGACAAGAT CAAGACCGTT 
GGTAGCACTT ATATGGCTGT CGTGGGACTC ATACCCGAGT ACAAAATCCA GCCTAACGAT 
CCGAACTCAG TGCGGCGCCA CATGACAGCG CTCATTGAGT ATGTCAAGGC CATGAGGCAC 
TCACTCCAAG AGATAAACAG TCACTCGTAC AATAACTTCA TGCTGCGCGT GGGCATCAAC 
ATTGGACCCG TTGTGGCGGG CGTAATTGGA GCTCGAAAGC CACAATATGA CATTTGGGGC 
AATACGGTGA ACGTGGCCAG TCGCATGGAC TCCACCGGAG TTCCCGGCTA CTCGCAAGTA 
ACTCAAGAGG TGGTCGACAG CCTGGTGGGG TCGCACTTTG AATTCCGCTG TCGTGGCACC 
ATTAAGGTGA AGGGCAAAGG CGACATGGTC ACATATTTCT TGTGTGACAG CGGCAACAAA 
TCGCTGAACG GAGAAGTGCG CAACGCCATG TCCCTGCCAC AGAGTCTCCA TGCGCCGGAT 
TACTATATGA AGGTGTCGCA GTTTCCGGAG AATCGCGTCA ATACCGACAC CTATAGCAAA 
AAGGAGAATG GACATCTGTA CGCTGGCAAC GGCGTCGAGG AGCAGCAGTT GTTGCTGCAG 
CATCAGCACA AACAGCACGA TCCGCTACCG CTTCCTGCTC CTCCGCCTCC GGTTCACCAT 
CATTTGCATC AGCAGCAACA GCAGCGTTTG AACAGCAAGC TGCAAAAGCA ACCGATCTTC 
ATGGCCAACG GTGGTCTGCC CAATATCCGG GAGAATGGCA ATGGTCACAA TGGAGAGCAT 
CAGCAGCAGC AGCAGCAGCA GCAGCAACAT CAGCAGCAGC AACAGCAGCA GCAGCAGCAC 
GGTGGCTTTA TGGTGGCAAC GACGACACCG CCGGCTGCAG TGGCGGTTCC CCTCCAGCCC 
CAGCACCATC AACTCCAGTT CCAGCACCCG CACCAGCATC CACTCCCGTC AGCAGTGTCG 
GTTCCAGTGC AGCACCAGAT CCTACTCCAC CACCAGCTCC AGCTCCAGCA CCAGCCCGTT 
CCTTCGATCA TGCCCATGCA GCATGCGCCA AAGTACGAGC CACCACGTTA CACTTCTCCC 
CACACCATGT TGTCGCAACA GCACCAGCAG CAGCAGCAGC AGCAGCATCA GCATCAGCAG 
CCGCAATCGC AATCTGCCCA GGATCAGCAG ACACATCCCG CCCAGGACCC ACATCCTTTG 
CAGCGGCAGT ACGCCATGTA TTCGCAGCAG CCGCAGCTGC CGCCGAAGCC AGTGCTGAGA 
ACGTACATGA AGCCGTTGCC GAAGCTGCCG ACGGATCTGG AGGAGAGCCG CGACATGTCC 
TCGACGGATG ATCTGAGCTC ACGACCACAC TCGCCCTCGA TGAGCAGCTC GGATGAGAGC 
TACTCGAAGA CCACAGAGGG CGAAGGTGAA GGCGATGAGG ACTCGCCGCG CATGGTCAAT 
GGTGGCCACC TGCATCATCG CAATGGCTAC CACCTGCCCG CCGGTGGTCT GGTCAACCCA 
TTGCAATGGC TCTACCCCTG CGACATTCAG GTGGATCCCA CCTCTCCGGT CGTCGACATG 
GCCCACCTGC ATGACTTTGA GCTAAGCTCC ACCACCGAGA GCCAGGGCCA TCACACTAAC 
AGCAACACGA CGAGCAACAC ACAACACAAG GGCGACAGCT GCAACAGCTT CGACTTTCAG 
AAGGCAGCAG TTGGAACGGC AGCGGGTGCG GCAATCGCGA CCAAGTCGCC CTTCGAGCGT 
GAACTGCAAC GTCTGCTTAA CGAATCCTCG CGAGCTCGTT GCCTGGCGAC TGCGACAACG 
ACGGCTGGAG CCATCTCTAC CACAGATCAG ACGGCCAGCA ATGGAAGCCG TGAGTTGAGC 
TACTCCCTCA GCAATGGGAA GCTGAGCTCT GCGAATGGAC ATGGCGTTGG AGGATCCGGA 
TCCGGATCCG GATCGGGATC AGGTTCAGGT TCGGCGGTGG GCAATGGCAG CGGTGGCAGC 
GGCAGCAGCA ATGGCAATCT CAGCGGCGGC AGCGGCAGCA ACTCGAACAG TGGCAACAAC 
AACAGCAGTC ACCACAAGAC GGAGCAGCAG CAGAATATGG ATCACGAGCA TCTGGCCGGT 
GGCAAATTGC TGGGCAGTAA TAGCTTCATG ATCGCCAAAC ATCCGGTGGG CTTGGAAGCC 
ATCAAGGAGA TAACGCGCAA CAAGAATCCC TCGGAGTCCA GCCAAATGCA AACCTCGGAC 
ACGGAGTCCT GCGAGATTCT GCACGAGAAC CGCAACCAGA TGCATGTGCT GGCCATGCTG 
GAGATGCATA CGGCCAAGGA GCTGAATGGC AGCCATGCGC ATCACGGTCA GCATCACCAG 
CAGCCACAGA GGACGCATCG CCAGCGACCG CGCTCCAAGG AGCTGCAGTA CAGCCACGAG 
AGTCTGGACG GACTGGATGG AGCGGTGCAG TCGCAGTCGC AGCAGCGACA CCAACGCTAT 
CATCACCACC ATCACCACCA ACAGAGGCAG CAGCAACAGC AGCGCTACAA CCATGTGCAG 
GAGCAGGAGG AGCGTGACGA CACCGAGGAC AACCTGGCCG ATGAGGAGTT TGAGGACGAC 
GAAGTGGGTC GTGATGTGCG ACAGAAGCGC CTACAAAAGT CCGAGCTCAA TCACAAACGA 
TCGGAGGTTG CAACAGAGGC AGGCAATCAC CACGACGACG AGGTGGAGGA GGAGGACGAC 
GATGACGACG AAGAGGAGGA CCACCGGAAT GGCGGGCGGG AGGCGGCACC GCTGACTAAC 
GGCAGCATGC GCGGCCTGGA GGCCAATGTG ATAAACGACG AGCTGAAGTA TGGAGCAACC 
CATCTCAATC ACCAGTCCAT GGACTCCAAT CCGTTGGAGA GCCAATCGGA GTGGTCGGAT 
GACGATTGTC GTGAGGAAGC AACAGGGGGA GCCGAGTCCA CGGGCTACAT TACGGATGAG 
CCCGGACTGG AGAACATCTC GTTGCTGAAC GAAGCTGGTC TCACCGATGC CGAGGGCGCT 
CTCTCGGATG TCAACTCGCT GTATAACGCG CCGGACGTGG ACGACACTTC GGTGTCCAGC 
CGGGCCAGCT CCCGCCTGCT TAGCCTGGAC TCGCTGAGCG GCCTCTACGA CTGCGATCTG 
GACTCCAAGC ACGAGCTGGC CATCGTCAAT GCCTCGCACA AGATCTCCAG TAAATTTGGG 
CAGCCTCTAT CACCGGCACA GCAGCAACAT CAACAACAGC AGCAACAGCA ACAGCAGCAA 
CAGCAGCAAC ACCACCAACA GCAGCTGCAG CAGAATCCGC AGCACACTCA AGCCCAATCG 
CATTTGGCTC CGGTTCAGTT TCAGTCCGCC GAGGAGTTGA GAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245672.1
CDS	331..6498
Translation	MDHAVKATRGRPLNTLRFENDELECLYQRYTLKLQRFSVLGVVALVFVLCGVMAALSLTFNNAATFHNIFNAIVCGLFAVVLVLLQCSVIKDHHLPTLCYGILLFTASICVVSMPTLGSVFPVDTKEVMAEGVWQIVFVVFLAYAMMPLQIWEAVAFGIALPSVHISLTVYKIFTDALRYLEYNQLIANIVIFIGVNVAGLVVNIMMERAQRRTFLDTRNCIASRLEIQDENEKLERLLLSVLPQHVAMQMKNDILSPVAGQFHRIYIQKHENVSILFADIVGFTVLSSQCSAQELVRLLNELFGRFDQLAHDNHCLRIKILGDCYYCVSGLPEPRKDHAKCAVEMGLDMIDAIATVVEATDVILNMRVGIHTGRVLCGVLGLRKWQFDVWSNDVTLANHMESGGEPGRVHVTRATLDSLSGEYEVEAGHGDERSSYLRDHGVDTFFIVPPPHRRKPLMLNTLGVRSAIGSRRKLSFRNVSNVVMQLLHTIKFSEPVPFSNIATGSFPSAASALGGGVSVGGGGGGGGGGVARGSTCEANSGNVQVSEKGSRKSQSKVADKFKRPFRKRHSVAAHHQPTNRVNRFLSQAINARSVDCDKSEHVDRLTLRFRQSDMEREYHKDFDLGFTTAMGCSLLLLILGAALQVTALPRTLILLLLFLFAFIWVSAILMLLLAVRLKWIIWDISESFSLRMAITIFTVILIYSVGQVNVFTCVSDHPCSGNGTTSFQNDSHRKCSLPQYVSLSAAFAFLSVSVFLRLPIIFKSLLVLGMGTIYGLFIELSHQNIFECYDNRVNASIPLHLISLARIAIFMIAILVHGRLVEGTARLDFLWQLQASQEKKEMDVLQESNKRILHNLLPAHVAAHFLDAQFRNNMELYHQSYAKVGVIFASVPNFNEFYTEMDGSDQGLECLRLLNEIIADFDELLKEDRFRGIDKIKTVGSTYMAVVGLIPEYKIQPNDPNSVRRHMTALIEYVKAMRHSLQEINSHSYNNFMLRVGINIGPVVAGVIGARKPQYDIWGNTVNVASRMDSTGVPGYSQVTQEVVDSLVGSHFEFRCRGTIKVKGKGDMVTYFLCDSGNKSLNGEVRNAMSLPQSLHAPDYYMKVSQFPENRVNTDTYSKKENGHLYAGNGVEEQQLLLQHQHKQHDPLPLPAPPPPVHHHLHQQQQQRLNSKLQKQPIFMANGGLPNIRENGNGHNGEHQQQQQQQQQHQQQQQQQQQHGGFMVATTTPPAAVAVPLQPQHHQLQFQHPHQHPLPSAVSVPVQHQILLHHQLQLQHQPVPSIMPMQHAPKYEPPRYTSPHTMLSQQHQQQQQQQHQHQQPQSQSAQDQQTHPAQDPHPLQRQYAMYSQQPQLPPKPVLRTYMKPLPKLPTDLEESRDMSSTDDLSSRPHSPSMSSSDESYSKTTEGEGEGDEDSPRMVNGGHLHHRNGYHLPAGGLVNPLQWLYPCDIQVDPTSPVVDMAHLHDFELSSTTESQGHHTNSNTTSNTQHKGDSCNSFDFQKAAVGTAAGAAIATKSPFERELQRLLNESSRARCLATATTTAGAISTTDQTASNGSRELSYSLSNGKLSSANGHGVGGSGSGSGSGSGSGSAVGNGSGGSGSSNGNLSGGSGSNSNSGNNNSSHHKTEQQQNMDHEHLAGGKLLGSNSFMIAKHPVGLEAIKEITRNKNPSESSQMQTSDTESCEILHENRNQMHVLAMLEMHTAKELNGSHAHHGQHHQQPQRTHRQRPRSKELQYSHESLDGLDGAVQSQSQQRHQRYHHHHHHQQRQQQQQRYNHVQEQEERDDTEDNLADEEFEDDEVGRDVRQKRLQKSELNHKRSEVATEAGNHHDDEVEEEDDDDDEEEDHRNGGREAAPLTNGSMRGLEANVINDELKYGATHLNHQSMDSNPLESQSEWSDDDCREEATGGAESTGYITDEPGLENISLLNEAGLTDAEGALSDVNSLYNAPDVDDTSVSSRASSRLLSLDSLSGLYDCDLDSKHELAIVNASHKISSKFGQPLSPAQQQHQQQQQQQQQQQQQHHQQQLQQNPQHTQAQSHLAPVQFQSAEELRE

Target ORF information:

RefSeq Version	NM_001258743.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster rutabaga (rut), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258743.3

ORF Insert Sequence:

ATGGATCATG CCGTGAAGGC GACGCGCGGC CGCCCGTTGA ATACGCTGCG CTTCGAGAAC 
GATGAGCTGG AATGCCTCTA CCAGCGGTAT ACGCTCAAGC TGCAGCGCTT CTCGGTGCTG 
GGCGTGGTGG CTCTGGTCTT CGTCCTCTGC GGCGTGATGG CGGCCCTTTC GCTGACCTTC 
AACAACGCGG CCACCTTTCA CAACATCTTC AATGCGATCG TTTGTGGACT GTTTGCCGTG 
GTGCTGGTGC TGCTGCAGTG TTCCGTGATC AAGGACCACC ACCTGCCCAC ATTGTGCTAC 
GGAATCCTGC TATTCACGGC CAGCATCTGT GTGGTGTCGA TGCCCACATT GGGCAGCGTG 
TTCCCGGTGG ACACCAAGGA GGTGATGGCC GAGGGCGTCT GGCAGATCGT GTTCGTGGTC 
TTCCTCGCTT ACGCCATGAT GCCGCTGCAG ATTTGGGAGG CGGTGGCCTT TGGCATCGCC 
CTGCCCTCGG TGCACATAAG TCTAACGGTC TATAAGATTT TCACGGATGC GCTGCGCTAC 
CTGGAGTATA ATCAACTGAT TGCGAACATT GTCATCTTTA TTGGTGTAAA TGTGGCCGGT 
CTCGTGGTGA ACATCATGAT GGAGCGCGCA CAGCGTCGCA CATTTCTCGA TACACGCAAC 
TGCATCGCAT CCCGTCTGGA GATCCAGGAT GAGAACGAGA AACTGGAACG TCTGTTGCTA 
TCGGTGCTGC CGCAGCACGT GGCCATGCAG ATGAAGAACG ACATCCTGTC GCCGGTGGCT 
GGGCAATTTC ATCGCATCTA CATTCAGAAG CACGAGAATG TGAGCATCCT TTTCGCGGAC 
ATTGTCGGAT TCACGGTGCT CTCCTCGCAG TGTAGTGCCC AGGAGCTGGT CCGCCTGCTC 
AACGAGCTCT TTGGCCGCTT CGATCAGCTG GCACACGATA ATCACTGTTT GCGTATCAAG 
ATCCTGGGCG ACTGTTACTA TTGTGTCTCC GGATTGCCAG AACCTCGCAA GGATCACGCC 
AAATGCGCCG TGGAAATGGG ACTCGATATG ATAGATGCCA TTGCCACCGT GGTGGAGGCC 
ACCGACGTCA TCCTGAACAT GCGCGTGGGC ATCCACACGG GCCGTGTGCT CTGCGGGGTC 
TTGGGCCTTC GCAAGTGGCA GTTCGACGTG TGGTCAAACG ATGTGACCCT GGCCAATCAC 
ATGGAGTCCG GCGGGGAACC GGGTCGGGTG CACGTAACCC GCGCCACCCT GGACTCCTTG 
TCCGGCGAAT ACGAGGTGGA GGCTGGCCAC GGCGACGAAC GGTCCTCGTA TCTGCGCGAC 
CATGGTGTGG ACACGTTCTT CATCGTTCCG CCGCCACATC GACGCAAGCC ACTGATGCTC 
AACACACTGG GCGTTCGCTC GGCGATCGGC AGTCGACGGA AGTTGTCGTT CAGAAACGTG 
TCCAATGTGG TCATGCAGCT GCTGCACACG ATCAAGTTCT CGGAACCGGT GCCGTTCTCC 
AATATAGCCA CCGGATCGTT CCCCTCAGCG GCCAGCGCCT TGGGTGGCGG AGTTTCAGTC 
GGCGGTGGTG GAGGAGGAGG AGGAGGAGGC GTGGCACGGG GTAGCACCTG CGAGGCGAAC 
TCTGGAAACG TTCAGGTGTC GGAGAAGGGA TCGCGCAAGT CCCAGTCGAA GGTGGCGGAC 
AAATTCAAGA GGCCATTCCG GAAGCGGCAC TCGGTGGCCG CCCACCATCA GCCAACGAAT 
CGGGTGAATC GATTCCTTTC CCAGGCGATC AATGCCCGGT CGGTGGACTG CGACAAATCG 
GAGCATGTGG ATCGATTGAC CCTGCGATTC CGACAGAGCG ACATGGAACG CGAGTATCAC 
AAGGATTTCG ATCTGGGCTT CACTACGGCC ATGGGTTGCT CCCTGCTGTT GCTCATCCTC 
GGTGCAGCGC TCCAGGTGAC TGCTCTGCCG CGCACTCTCA TCCTGCTGCT GCTCTTTCTC 
TTTGCCTTCA TTTGGGTGAG CGCCATTCTG ATGCTCCTGC TCGCCGTGCG TCTCAAATGG 
ATCATCTGGG ATATTTCGGA GAGCTTTTCG CTGCGCATGG CCATCACGAT TTTCACGGTC 
ATACTCATCT ATTCCGTGGG ACAAGTGAAT GTGTTTACGT GCGTTTCGGA TCATCCATGC 
TCGGGCAATG GGACGACCTC CTTCCAGAAC GACTCTCATC GCAAGTGCTC GCTGCCGCAG 
TACGTGTCCC TATCGGCTGC CTTTGCCTTT CTATCTGTAT CGGTATTCCT GAGGCTGCCC 
ATCATATTCA AATCGCTCCT AGTTCTGGGC ATGGGCACCA TCTATGGCCT TTTCATAGAG 
CTATCGCATC AGAATATCTT TGAGTGCTAC GACAACCGGG TCAACGCCTC GATTCCACTT 
CACTTGATCT CGCTGGCCCG CATTGCCATC TTCATGATTG CCATTTTGGT GCATGGACGC 
CTGGTGGAGG GCACTGCCCG GCTGGACTTC CTCTGGCAGC TGCAGGCGTC GCAGGAGAAG 
AAGGAGATGG ATGTGCTACA GGAGTCCAAT AAGCGCATTC TACACAATTT GCTGCCCGCT 
CATGTGGCTG CACATTTTCT CGATGCGCAA TTTCGCAACA ACATGGAACT CTACCACCAG 
AGCTATGCCA AAGTGGGCGT CATCTTTGCA AGCGTACCGA ACTTCAACGA GTTTTACACG 
GAAATGGATG GCTCCGATCA GGGTCTTGAG TGCCTCCGTC TGCTCAACGA GATTATTGCG 
GACTTTGATG AGCTGCTCAA GGAGGATCGT TTCCGCGGCA TCGACAAGAT CAAGACCGTT 
GGTAGCACTT ATATGGCTGT CGTGGGACTC ATACCCGAGT ACAAAATCCA GCCTAACGAT 
CCGAACTCAG TGCGGCGCCA CATGACAGCG CTCATTGAGT ATGTCAAGGC CATGAGGCAC 
TCACTCCAAG AGATAAACAG TCACTCGTAC AATAACTTCA TGCTGCGCGT GGGCATCAAC 
ATTGGACCCG TTGTGGCGGG CGTAATTGGA GCTCGAAAGC CACAATATGA CATTTGGGGC 
AATACGGTGA ACGTGGCCAG TCGCATGGAC TCCACCGGAG TTCCCGGCTA CTCGCAAGTA 
ACTCAAGAGG TGGTCGACAG CCTGGTGGGG TCGCACTTTG AATTCCGCTG TCGTGGCACC 
ATTAAGGTGA AGGGCAAAGG CGACATGGTC ACATATTTCT TGTGTGACAG CGGCAACAAA 
TCGCTGAACG GAGAAGTGCG CAACGCCATG TCCCTGCCAC AGAGTCTCCA TGCGCCGGAT 
TACTATATGA AGGTGTCGCA GTTTCCGGAG AATCGCGTCA ATACCGACAC CTATAGCAAA 
AAGGAGAATG GACATCTGTA CGCTGGCAAC GGCGTCGAGG AGCAGCAGTT GTTGCTGCAG 
CATCAGCACA AACAGCACGA TCCGCTACCG CTTCCTGCTC CTCCGCCTCC GGTTCACCAT 
CATTTGCATC AGCAGCAACA GCAGCGTTTG AACAGCAAGC TGCAAAAGCA ACCGATCTTC 
ATGGCCAACG GTGGTCTGCC CAATATCCGG GAGAATGGCA ATGGTCACAA TGGAGAGCAT 
CAGCAGCAGC AGCAGCAGCA GCAGCAACAT CAGCAGCAGC AACAGCAGCA GCAGCAGCAC 
GGTGGCTTTA TGGTGGCAAC GACGACACCG CCGGCTGCAG TGGCGGTTCC CCTCCAGCCC 
CAGCACCATC AACTCCAGTT CCAGCACCCG CACCAGCATC CACTCCCGTC AGCAGTGTCG 
GTTCCAGTGC AGCACCAGAT CCTACTCCAC CACCAGCTCC AGCTCCAGCA CCAGCCCGTT 
CCTTCGATCA TGCCCATGCA GCATGCGCCA AAGTACGAGC CACCACGTTA CACTTCTCCC 
CACACCATGT TGTCGCAACA GCACCAGCAG CAGCAGCAGC AGCAGCATCA GCATCAGCAG 
CCGCAATCGC AATCTGCCCA GGATCAGCAG ACACATCCCG CCCAGGACCC ACATCCTTTG 
CAGCGGCAGT ACGCCATGTA TTCGCAGCAG CCGCAGCTGC CGCCGAAGCC AGTGCTGAGA 
ACGTACATGA AGCCGTTGCC GAAGCTGCCG ACGGATCTGG AGGAGAGCCG CGACATGTCC 
TCGACGGATG ATCTGAGCTC ACGACCACAC TCGCCCTCGA TGAGCAGCTC GGATGAGAGC 
TACTCGAAGA CCACAGAGGG CGAAGGTGAA GGCGATGAGG ACTCGCCGCG CATGGTCAAT 
GGTGGCCACC TGCATCATCG CAATGGCTAC CACCTGCCCG CCGGTGGTCT GGTCAACCCA 
TTGCAATGGC TCTACCCCTG CGACATTCAG GTGGATCCCA CCTCTCCGGT CGTCGACATG 
GCCCACCTGC ATGACTTTGA GCTAAGCTCC ACCACCGAGA GCCAGGGCCA TCACACTAAC 
AGCAACACGA CGAGCAACAC ACAACACAAG GGCGACAGCT GCAACAGCTT CGACTTTCAG 
AAGGCAGCAG TTGGAACGGC AGCGGGTGCG GCAATCGCGA CCAAGTCGCC CTTCGAGCGT 
GAACTGCAAC GTCTGCTTAA CGAATCCTCG CGAGCTCGTT GCCTGGCGAC TGCGACAACG 
ACGGCTGGAG CCATCTCTAC CACAGATCAG ACGGCCAGCA ATGGAAGCCG TGAGTTGAGC 
TACTCCCTCA GCAATGGGAA GCTGAGCTCT GCGAATGGAC ATGGCGTTGG AGGATCCGGA 
TCCGGATCCG GATCGGGATC AGGTTCAGGT TCGGCGGTGG GCAATGGCAG CGGTGGCAGC 
GGCAGCAGCA ATGGCAATCT CAGCGGCGGC AGCGGCAGCA ACTCGAACAG TGGCAACAAC 
AACAGCAGTC ACCACAAGAC GGAGCAGCAG CAGAATATGG ATCACGAGCA TCTGGCCGGT 
GGCAAATTGC TGGGCAGTAA TAGCTTCATG ATCGCCAAAC ATCCGGTGGG CTTGGAAGCC 
ATCAAGGAGA TAACGCGCAA CAAGAATCCC TCGGAGTCCA GCCAAATGCA AACCTCGGAC 
ACGGAGTCCT GCGAGATTCT GCACGAGAAC CGCAACCAGA TGCATGTGCT GGCCATGCTG 
GAGATGCATA CGGCCAAGGA GCTGAATGGC AGCCATGCGC ATCACGGTCA GCATCACCAG 
CAGCCACAGA GGACGCATCG CCAGCGACCG CGCTCCAAGG AGCTGCAGTA CAGCCACGAG 
AGTCTGGACG GACTGGATGG AGCGGTGCAG TCGCAGTCGC AGCAGCGACA CCAACGCTAT 
CATCACCACC ATCACCACCA ACAGAGGCAG CAGCAACAGC AGCGCTACAA CCATGTGCAG 
GAGCAGGAGG AGCGTGACGA CACCGAGGAC AACCTGGCCG ATGAGGAGTT TGAGGACGAC 
GAAGTGGGTC GTGATGTGCG ACAGAAGCGC CTACAAAAGT CCGAGCTCAA TCACAAACGA 
TCGGAGGTTG CAACAGAGGC AGGCAATCAC CACGACGACG AGGTGGAGGA GGAGGACGAC 
GATGACGACG AAGAGGAGGA CCACCGGAAT GGCGGGCGGG AGGCGGCACC GCTGACTAAC 
GGCAGCATGC GCGGCCTGGA GGCCAATGTG ATAAACGACG AGCTGAAGTA TGGAGCAACC 
CATCTCAATC ACCAGTCCAT GGACTCCAAT CCGTTGGAGA GCCAATCGGA GTGGTCGGAT 
GACGATTGTC GTGAGGAAGC AACAGGGGGA GCCGAGTCCA CGGGCTACAT TACGGATGAG 
CCCGGACTGG AGAACATCTC GTTGCTGAAC GAAGCTGGTC TCACCGATGC CGAGGGCGCT 
CTCTCGGATG TCAACTCGCT GTATAACGCG CCGGACGTGG ACGACACTTC GGTGTCCAGC 
CGGGCCAGCT CCCGCCTGCT TAGCCTGGAC TCGCTGAGCG GCCTCTACGA CTGCGATCTG 
GACTCCAAGC ACGAGCTGGC CATCGTCAAT GCCTCGCACA AGATCTCCAG TAAATTTGGG 
CAGCCTCTAT CACCGGCACA GCAGCAACAT CAACAACAGC AGCAACAGCA ACAGCAGCAA 
CAGCAGCAAC ACCACCAACA GCAGCTGCAG CAGAATCCGC AGCACACTCA AGCCCAATCG 
CATTTGGCTC CGGTTCAGTT TCAGTCCGCC GAGGAGTTGA GAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rut ( NM_001272619.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259548.1 Drosophila melanogaster rutabaga (rut), transcript variant C, mRNA.

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CloneID	OFa03565
Clone ID Related Accession (Same CDS sequence)	NM_001272619.2
Accession Version	NM_001272619.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	rutabaga, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001272619.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATCATG CCGTGAAGGC GACGCGCGGC CGCCCGTTGA ATACGCTGCG CTTCGAGAAC 
GATGAGCTGG AATGCCTCTA CCAGCGGTAT ACGCTCAAGC TGCAGCGCTT CTCGGTGCTG 
GGCGTGGTGG CTCTGGTCTT CGTCCTCTGC GGCGTGATGG CGGCCCTTTC GCTGACCTTC 
AACAACGCGG CCACCTTTCA CAACATCTTC AATGCGATCG TTTGTGGACT GTTTGCCGTG 
GTGCTGGTGC TGCTGCAGTG TTCCGTGATC AAGGACCACC ACCTGCCCAC ATTGTGCTAC 
GGAATCCTGC TATTCACGGC CAGCATCTGT GTGGTGTCGA TGCCCACATT GGGCAGCGTG 
TTCCCGGTGG ACACCAAGGA GGTGATGGCC GAGGGCGTCT GGCAGATCGT GTTCGTGGTC 
TTCCTCGCTT ACGCCATGAT GCCGCTGCAG ATTTGGGAGG CGGTGGCCTT TGGCATCGCC 
CTGCCCTCGG TGCACATAAG TCTAACGGTC TATAAGATTT TCACGGATGC GCTGCGCTAC 
CTGGAGTATA ATCAACTGAT TGCGAACATT GTCATCTTTA TTGGTGTAAA TGTGGCCGGT 
CTCGTGGTGA ACATCATGAT GGAGCGCGCA CAGCGTCGCA CATTTCTCGA TACACGCAAC 
TGCATCGCAT CCCGTCTGGA GATCCAGGAT GAGAACGAGA AACTGGAACG TCTGTTGCTA 
TCGGTGCTGC CGCAGCACGT GGCCATGCAG ATGAAGAACG ACATCCTGTC GCCGGTGGCT 
GGGCAATTTC ATCGCATCTA CATTCAGAAG CACGAGAATG TGAGCATCCT TTTCGCGGAC 
ATTGTCGGAT TCACGGTGCT CTCCTCGCAG TGTAGTGCCC AGGAGCTGGT CCGCCTGCTC 
AACGAGCTCT TTGGCCGCTT CGATCAGCTG GCACACGATA ATCACTGTTT GCGTATCAAG 
ATCCTGGGCG ACTGTTACTA TTGTGTCTCC GGATTGCCAG AACCTCGCAA GGATCACGCC 
AAATGCGCCG TGGAAATGGG ACTCGATATG ATAGATGCCA TTGCCACCGT GGTGGAGGCC 
ACCGACGTCA TCCTGAACAT GCGCGTGGGC ATCCACACGG GCCGTGTGCT CTGCGGGGTC 
TTGGGCCTTC GCAAGTGGCA GTTCGACGTG TGGTCAAACG ATGTGACCCT GGCCAATCAC 
ATGGAGTCCG GCGGGGAACC GGGTCGGGTG CACGTAACCC GCGCCACCCT GGACTCCTTG 
TCCGGCGAAT ACGAGGTGGA GGCTGGCCAC GGCGACGAAC GGTCCTCGTA TCTGCGCGAC 
CATGGTGTGG ACACGTTCTT CATCGTTCCG CCGCCACATC GACGCAAGCC ACTGATGCTC 
AACACACTGG GCGTTCGCTC GGCGATCGGC AGTCGACGGA AGTTGTCGTT CAGAAACGTG 
TCCAATGTGG TCATGCAGCT GCTGCACACG ATCAAGTTCT CGGAACCGGT GCCGTTCTCC 
AATATAGCCA CCGGATCGTT CCCCTCAGCG GCCAGCGCCT TGGGTGGCGG AGTTTCAGTC 
GGCGGTGGTG GAGGAGGAGG AGGAGGAGGC GTGGCACGGG GTAGCACCTG CGAGGCGAAC 
TCTGGAAACG TTCAGGTGTC GGAGAAGGGA TCGCGCAAGT CCCAGTCGAA GGTGGCGGAC 
AAATTCAAGA GGCCATTCCG GAAGCGGCAC TCGGTGGCCG CCCACCATCA GCCAACGAAT 
CGGGTGAATC GATTCCTTTC CCAGGCGATC AATGCCCGGT CGGTGGACTG CGACAAATCG 
GAGCATGTGG ATCGATTGAC CCTGCGATTC CGACAGAGCG ACATGGAACG CGAGTATCAC 
AAGGATTTCG ATCTGGGCTT CACTACGGCC ATGGGTTGCT CCCTGCTGTT GCTCATCCTC 
GGTGCAGCGC TCCAGGTGAC TGCTCTGCCG CGCACTCTCA TCCTGCTGCT GCTCTTTCTC 
TTTGCCTTCA TTTGGGTGAG CGCCATTCTG ATGCTCCTGC TCGCCGTGCG TCTCAAATGG 
ATCATCTGGG ATATTTCGGA GAGCTTTTCG CTGCGCATGG CCATCACGAT TTTCACGGTC 
ATACTCATCT ATTCCGTGGG ACAAGTGAAT GTGTTTACGT GCGTTTCGGA TCATCCATGC 
TCGGGCAATG GGACGACCTC CTTCCAGAAC GACTCTCATC GCAAGTGCTC GCTGCCGCAG 
TACGTGTCCC TATCGGCTGC CTTTGCCTTT CTATCTGTAT CGGTATTCCT GAGGCTGCCC 
ATCATATTCA AATCGCTCCT AGTTCTGGGC ATGGGCACCA TCTATGGCCT TTTCATAGAG 
CTATCGCATC AGAATATCTT TGAGTGCTAC GACAACCGGG TCAACGCCTC GATTCCACTT 
CACTTGATCT CGCTGGCCCG CATTGCCATC TTCATGATTG CCATTTTGGT GCATGGACGC 
CTGGTGGAGG GCACTGCCCG GCTGGACTTC CTCTGGCAGC TGCAGGCGTC GCAGGAGAAG 
AAGGAGATGG ATGTGCTACA GGAGTCCAAT AAGCGCATTC TACACAATTT GCTGCCCGCT 
CATGTGGCTG CACATTTTCT CGATGCGCAA TTTCGCAACA ACATGGAACT CTACCACCAG 
AGCTATGCCA AAGTGGGCGT CATCTTTGCA AGCGTACCGA ACTTCAACGA GTTTTACACG 
GAAATGGATG GCTCCGATCA GGGTCTTGAG TGCCTCCGTC TGCTCAACGA GATTATTGCG 
GACTTTGATG AGCTGCTCAA GGAGGATCGT TTCCGCGGCA TCGACAAGAT CAAGACCGTT 
GGTAGCACTT ATATGGCTGT CGTGGGACTC ATACCCGAGT ACAAAATCCA GCCTAACGAT 
CCGAACTCAG TGCGGCGCCA CATGACAGCG CTCATTGAGT ATGTCAAGGC CATGAGGCAC 
TCACTCCAAG AGATAAACAG TCACTCGTAC AATAACTTCA TGCTGCGCGT GGGCATCAAC 
ATTGGACCCG TTGTGGCGGG CGTAATTGGA GCTCGAAAGC CACAATATGA CATTTGGGGC 
AATACGGTGA ACGTGGCCAG TCGCATGGAC TCCACCGGAG TTCCCGGCTA CTCGCAAGTA 
ACTCAAGAGG TGGTCGACAG CCTGGTGGGG TCGCACTTTG AATTCCGCTG TCGTGGCACC 
ATTAAGGTGA AGGGCAAAGG CGACATGGTC ACATATTTCT TGTGTGACAG CGGCAACAAA 
TCGCTGAACG GAGAAGTGCG CAACGCCATG TCCCTGCCAC AGAGTCTCCA TGCGCCGGAT 
TACTATATGA AGGTGTCGCA GTTTCCGGAG AATCGCGTCA ATACCGACAC CTATAGCAAA 
AAGGAGAATG GACATCTGTA CGCTGGCAAC GGCGTCGAGG AGCAGCAGTT GTTGCTGCAG 
CATCAGCACA AACAGCACGA TCCGCTACCG CTTCCTGCTC CTCCGCCTCC GGTTCACCAT 
CATTTGCATC AGCAGCAACA GCAGCGTTTG AACAGCAAGC TGCAAAAGCA ACCGATCTTC 
ATGGCCAACG GTGGTCTGCC CAATATCCGG GAGAATGGCA ATGGTCACAA TGGAGAGCAT 
CAGCAGCAGC AGCAGCAGCA GCAGCAACAT CAGCAGCAGC AACAGCAGCA GCAGCAGCAC 
GGTGGCTTTA TGGTGGCAAC GACGACACCG CCGGCTGCAG TGGCGGTTCC CCTCCAGCCC 
CAGCACCATC AACTCCAGTT CCAGCACCCG CACCAGCATC CACTCCCGTC AGCAGTGTCG 
GTTCCAGTGC AGCACCAGAT CCTACTCCAC CACCAGCTCC AGCTCCAGCA CCAGCCCGTT 
CCTTCGGTAA TGTTGCGCGA GTTTAATATT ATTGAGAATC CGACTTCCGG TGGCCGGCAT 
CAGCAGATGG AGCAGCTGCC GCCGCATCAC GGCAGCCTCG ACCTATCGGG AATGGGAATG 
GGTGTGGGGG CTGGCGTGCT CGGCAGCGAC TGCTTCATGA TGCCACGGCG AGATCGCGAA 
CGCACATATG TGCCGCCCTT AAACCAGCAT GGCCATCATC CACCGCATCA CCTCCATTCG 
AATCTGAATC TGAATCAGAG CCAGCACCCG CCATCGTTCA CCAGCCTGGG CTATGGCCAA 
TGCCGGGAGA GCGAGCCGCT ATTGCATGCC AGCAGCGTCG CGCCGGTAGC CAAGCACCAG 
CAGCAGCAGC AGCAGCAGCA TCAGCATCAG CAGCCGCAAT CGCAATCTGC CCAGGATCAG 
CAGACACATC CCGCCCAGGA CCCACATCCT TTGCAGCGGC AGTACGCCAT GTATTCGCAG 
CAGCCGCAGC TGCCGCCGAA GCCAGTGCTG AGAACGTACA TGAAGCCGTT GCCGAAGCTG 
CCGACGGATC TGGAGGAGAG CCGCGACATG TCCTCGACGG ATGATCTGAG CTCACGACCA 
CACTCGCCCT CGATGAGCAG CTCGGATGAG AGCTACTCGA AGACCACAGA GGGCGAAGGT 
GAAGGCGATG AGGACTCGCC GCGCATGGTC AATGGTGGCC ACCTGCATCA TCGCAATGGC 
TACCACCTGC CCGCCGGTGG TCTGGTCAAC CCATTGCAAT GGCTCTACCC CTGCGACATT 
CAGGTGGATC CCACCTCTCC GGTCGTCGAC ATGGCCCACC TGCATGACTT TGAGCTAAGC 
TCCACCACCG AGAGCCAGGG CCATCACACT AACAGCAACA CGACGAGCAA CACACAACAC 
AAGGGCGACA GCTGCAACAG CTTCGACTTT CAGAAGGCAG CAGTTGGAAC GGCAGCGGGT 
GCGGCAATCG CGACCAAGTC GCCCTTCGAG CGTGAACTGC AACGTCTGCT TAACGAATCC 
TCGCGAGCTC GTTGCCTGGC GACTGCGACA ACGACGGCTG GAGCCATCTC TACCACAGAT 
CAGACGGCCA GCAATGGAAG CCGTGAGTTG AGCTACTCCC TCAGCAATGG GAAGCTGAGC 
TCTGCGAATG GACATGGCGT TGGAGGATCC GGATCCGGAT CCGGATCGGG ATCAGGTTCA 
GGTTCGGCGG TGGGCAATGG CAGCGGTGGC AGCGGCAGCA GCAATGGCAA TCTCAGCGGC 
GGCAGCGGCA GCAACTCGAA CAGTGGCAAC AACAACAGCA GTCACCACAA GACGGAGCAG 
CAGCAGAATA TGGATCACGA GCATCTGGCC GGTGGCAAAT TGCTGGGCAG TAATAGCTTC 
ATGATCGCCA AACATCCGGT GGGCTTGGAA GCCATCAAGG AGATAACGCG CAACAAGAAT 
CCCTCGGAGT CCAGCCAAAT GCAAACCTCG GACACGGAGT CCTGCGAGAT TCTGCACGAG 
AACCGCAACC AGATGCATGT GCTGGCCATG CTGGAGATGC ATACGGCCAA GGAGCTGAAT 
GGCAGCCATG CGCATCACGG TCAGCATCAC CAGCAGCCAC AGAGGACGCA TCGCCAGCGA 
CCGCGCTCCA AGGAGCTGCA GTACAGCCAC GAGAGTCTGG ACGGACTGGA TGGAGCGGTG 
CAGTCGCAGT CGCAGCAGCG ACACCAACGC TATCATCACC ACCATCACCA CCAACAGAGG 
CAGCAGCAAC AGCAGCGCTA CAACCATGTG CAGGAGCAGG AGGAGCGTGA CGACACCGAG 
GACAACCTGG CCGATGAGGA GTTTGAGGAC GACGAAGTGG GTCGTGATGT GCGACAGAAG 
CGCCTACAAA AGTCCGAGCT CAATCACAAA CGATCGGAGG TTGCAACAGA GGCAGGCAAT 
CACCACGACG ACGAGGTGGA GGAGGAGGAC GACGATGACG ACGAAGAGGA GGACCACCGG 
AATGGCGGGC GGGAGGCGGC ACCGCTGACT AACGGCAGCA TGCGCGGCCT GGAGGCCAAT 
GTGATAAACG ACGAGCTGAA GTATGGAGCA ACCCATCTCA ATCACCAGTC CATGGACTCC 
AATCCGTTGG AGAGCCAATC GGAGTGGTCG GATGACGATT GTCGTGAGGA AGCAACAGGG 
GGAGCCGAGT CCACGGGCTA CATTACGGAT GAGCCCGGAC TGGAGAACAT CTCGTTGCTG 
AACGAAGCTG GTCTCACCGA TGCCGAGGGC GCTCTCTCGG ATGTCAACTC GCTGTATAAC 
GCGCCGGACG TGGACGACAC TTCGGTGTCC AGCCGGGCCA GCTCCCGCCT GCTTAGCCTG 
GACTCGCTGA GCGGCCTCTA CGACTGCGAT CTGGACTCCA AGCACGAGCT GGCCATCGTC 
AATGCCTCGC ACAAGATCTC CAGTAAATTT GGGCAGCCTC TATCACCGGC ACAGCAGCAA 
CATCAACAAC AGCAGCAACA GCAACAGCAG CAACAGCAGC AACACCACCA ACAGCAGCTG 
CAGCAGAATC CGCAGCACAC TCAAGCCCAA TCGCATTTGG CTCCGGTTCA GTTTCAGTCC 
GCCGAGGAGT TGAGAGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259548.1
CDS	331..6771
Translation	MDHAVKATRGRPLNTLRFENDELECLYQRYTLKLQRFSVLGVVALVFVLCGVMAALSLTFNNAATFHNIFNAIVCGLFAVVLVLLQCSVIKDHHLPTLCYGILLFTASICVVSMPTLGSVFPVDTKEVMAEGVWQIVFVVFLAYAMMPLQIWEAVAFGIALPSVHISLTVYKIFTDALRYLEYNQLIANIVIFIGVNVAGLVVNIMMERAQRRTFLDTRNCIASRLEIQDENEKLERLLLSVLPQHVAMQMKNDILSPVAGQFHRIYIQKHENVSILFADIVGFTVLSSQCSAQELVRLLNELFGRFDQLAHDNHCLRIKILGDCYYCVSGLPEPRKDHAKCAVEMGLDMIDAIATVVEATDVILNMRVGIHTGRVLCGVLGLRKWQFDVWSNDVTLANHMESGGEPGRVHVTRATLDSLSGEYEVEAGHGDERSSYLRDHGVDTFFIVPPPHRRKPLMLNTLGVRSAIGSRRKLSFRNVSNVVMQLLHTIKFSEPVPFSNIATGSFPSAASALGGGVSVGGGGGGGGGGVARGSTCEANSGNVQVSEKGSRKSQSKVADKFKRPFRKRHSVAAHHQPTNRVNRFLSQAINARSVDCDKSEHVDRLTLRFRQSDMEREYHKDFDLGFTTAMGCSLLLLILGAALQVTALPRTLILLLLFLFAFIWVSAILMLLLAVRLKWIIWDISESFSLRMAITIFTVILIYSVGQVNVFTCVSDHPCSGNGTTSFQNDSHRKCSLPQYVSLSAAFAFLSVSVFLRLPIIFKSLLVLGMGTIYGLFIELSHQNIFECYDNRVNASIPLHLISLARIAIFMIAILVHGRLVEGTARLDFLWQLQASQEKKEMDVLQESNKRILHNLLPAHVAAHFLDAQFRNNMELYHQSYAKVGVIFASVPNFNEFYTEMDGSDQGLECLRLLNEIIADFDELLKEDRFRGIDKIKTVGSTYMAVVGLIPEYKIQPNDPNSVRRHMTALIEYVKAMRHSLQEINSHSYNNFMLRVGINIGPVVAGVIGARKPQYDIWGNTVNVASRMDSTGVPGYSQVTQEVVDSLVGSHFEFRCRGTIKVKGKGDMVTYFLCDSGNKSLNGEVRNAMSLPQSLHAPDYYMKVSQFPENRVNTDTYSKKENGHLYAGNGVEEQQLLLQHQHKQHDPLPLPAPPPPVHHHLHQQQQQRLNSKLQKQPIFMANGGLPNIRENGNGHNGEHQQQQQQQQQHQQQQQQQQQHGGFMVATTTPPAAVAVPLQPQHHQLQFQHPHQHPLPSAVSVPVQHQILLHHQLQLQHQPVPSVMLREFNIIENPTSGGRHQQMEQLPPHHGSLDLSGMGMGVGAGVLGSDCFMMPRRDRERTYVPPLNQHGHHPPHHLHSNLNLNQSQHPPSFTSLGYGQCRESEPLLHASSVAPVAKHQQQQQQQHQHQQPQSQSAQDQQTHPAQDPHPLQRQYAMYSQQPQLPPKPVLRTYMKPLPKLPTDLEESRDMSSTDDLSSRPHSPSMSSSDESYSKTTEGEGEGDEDSPRMVNGGHLHHRNGYHLPAGGLVNPLQWLYPCDIQVDPTSPVVDMAHLHDFELSSTTESQGHHTNSNTTSNTQHKGDSCNSFDFQKAAVGTAAGAAIATKSPFERELQRLLNESSRARCLATATTTAGAISTTDQTASNGSRELSYSLSNGKLSSANGHGVGGSGSGSGSGSGSGSAVGNGSGGSGSSNGNLSGGSGSNSNSGNNNSSHHKTEQQQNMDHEHLAGGKLLGSNSFMIAKHPVGLEAIKEITRNKNPSESSQMQTSDTESCEILHENRNQMHVLAMLEMHTAKELNGSHAHHGQHHQQPQRTHRQRPRSKELQYSHESLDGLDGAVQSQSQQRHQRYHHHHHHQQRQQQQQRYNHVQEQEERDDTEDNLADEEFEDDEVGRDVRQKRLQKSELNHKRSEVATEAGNHHDDEVEEEDDDDDEEEDHRNGGREAAPLTNGSMRGLEANVINDELKYGATHLNHQSMDSNPLESQSEWSDDDCREEATGGAESTGYITDEPGLENISLLNEAGLTDAEGALSDVNSLYNAPDVDDTSVSSRASSRLLSLDSLSGLYDCDLDSKHELAIVNASHKISSKFGQPLSPAQQQHQQQQQQQQQQQQQHHQQQLQQNPQHTQAQSHLAPVQFQSAEELRE

Target ORF information:

RefSeq Version	NM_001272619.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster rutabaga (rut), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272619.2

ORF Insert Sequence:

ATGGATCATG CCGTGAAGGC GACGCGCGGC CGCCCGTTGA ATACGCTGCG CTTCGAGAAC 
GATGAGCTGG AATGCCTCTA CCAGCGGTAT ACGCTCAAGC TGCAGCGCTT CTCGGTGCTG 
GGCGTGGTGG CTCTGGTCTT CGTCCTCTGC GGCGTGATGG CGGCCCTTTC GCTGACCTTC 
AACAACGCGG CCACCTTTCA CAACATCTTC AATGCGATCG TTTGTGGACT GTTTGCCGTG 
GTGCTGGTGC TGCTGCAGTG TTCCGTGATC AAGGACCACC ACCTGCCCAC ATTGTGCTAC 
GGAATCCTGC TATTCACGGC CAGCATCTGT GTGGTGTCGA TGCCCACATT GGGCAGCGTG 
TTCCCGGTGG ACACCAAGGA GGTGATGGCC GAGGGCGTCT GGCAGATCGT GTTCGTGGTC 
TTCCTCGCTT ACGCCATGAT GCCGCTGCAG ATTTGGGAGG CGGTGGCCTT TGGCATCGCC 
CTGCCCTCGG TGCACATAAG TCTAACGGTC TATAAGATTT TCACGGATGC GCTGCGCTAC 
CTGGAGTATA ATCAACTGAT TGCGAACATT GTCATCTTTA TTGGTGTAAA TGTGGCCGGT 
CTCGTGGTGA ACATCATGAT GGAGCGCGCA CAGCGTCGCA CATTTCTCGA TACACGCAAC 
TGCATCGCAT CCCGTCTGGA GATCCAGGAT GAGAACGAGA AACTGGAACG TCTGTTGCTA 
TCGGTGCTGC CGCAGCACGT GGCCATGCAG ATGAAGAACG ACATCCTGTC GCCGGTGGCT 
GGGCAATTTC ATCGCATCTA CATTCAGAAG CACGAGAATG TGAGCATCCT TTTCGCGGAC 
ATTGTCGGAT TCACGGTGCT CTCCTCGCAG TGTAGTGCCC AGGAGCTGGT CCGCCTGCTC 
AACGAGCTCT TTGGCCGCTT CGATCAGCTG GCACACGATA ATCACTGTTT GCGTATCAAG 
ATCCTGGGCG ACTGTTACTA TTGTGTCTCC GGATTGCCAG AACCTCGCAA GGATCACGCC 
AAATGCGCCG TGGAAATGGG ACTCGATATG ATAGATGCCA TTGCCACCGT GGTGGAGGCC 
ACCGACGTCA TCCTGAACAT GCGCGTGGGC ATCCACACGG GCCGTGTGCT CTGCGGGGTC 
TTGGGCCTTC GCAAGTGGCA GTTCGACGTG TGGTCAAACG ATGTGACCCT GGCCAATCAC 
ATGGAGTCCG GCGGGGAACC GGGTCGGGTG CACGTAACCC GCGCCACCCT GGACTCCTTG 
TCCGGCGAAT ACGAGGTGGA GGCTGGCCAC GGCGACGAAC GGTCCTCGTA TCTGCGCGAC 
CATGGTGTGG ACACGTTCTT CATCGTTCCG CCGCCACATC GACGCAAGCC ACTGATGCTC 
AACACACTGG GCGTTCGCTC GGCGATCGGC AGTCGACGGA AGTTGTCGTT CAGAAACGTG 
TCCAATGTGG TCATGCAGCT GCTGCACACG ATCAAGTTCT CGGAACCGGT GCCGTTCTCC 
AATATAGCCA CCGGATCGTT CCCCTCAGCG GCCAGCGCCT TGGGTGGCGG AGTTTCAGTC 
GGCGGTGGTG GAGGAGGAGG AGGAGGAGGC GTGGCACGGG GTAGCACCTG CGAGGCGAAC 
TCTGGAAACG TTCAGGTGTC GGAGAAGGGA TCGCGCAAGT CCCAGTCGAA GGTGGCGGAC 
AAATTCAAGA GGCCATTCCG GAAGCGGCAC TCGGTGGCCG CCCACCATCA GCCAACGAAT 
CGGGTGAATC GATTCCTTTC CCAGGCGATC AATGCCCGGT CGGTGGACTG CGACAAATCG 
GAGCATGTGG ATCGATTGAC CCTGCGATTC CGACAGAGCG ACATGGAACG CGAGTATCAC 
AAGGATTTCG ATCTGGGCTT CACTACGGCC ATGGGTTGCT CCCTGCTGTT GCTCATCCTC 
GGTGCAGCGC TCCAGGTGAC TGCTCTGCCG CGCACTCTCA TCCTGCTGCT GCTCTTTCTC 
TTTGCCTTCA TTTGGGTGAG CGCCATTCTG ATGCTCCTGC TCGCCGTGCG TCTCAAATGG 
ATCATCTGGG ATATTTCGGA GAGCTTTTCG CTGCGCATGG CCATCACGAT TTTCACGGTC 
ATACTCATCT ATTCCGTGGG ACAAGTGAAT GTGTTTACGT GCGTTTCGGA TCATCCATGC 
TCGGGCAATG GGACGACCTC CTTCCAGAAC GACTCTCATC GCAAGTGCTC GCTGCCGCAG 
TACGTGTCCC TATCGGCTGC CTTTGCCTTT CTATCTGTAT CGGTATTCCT GAGGCTGCCC 
ATCATATTCA AATCGCTCCT AGTTCTGGGC ATGGGCACCA TCTATGGCCT TTTCATAGAG 
CTATCGCATC AGAATATCTT TGAGTGCTAC GACAACCGGG TCAACGCCTC GATTCCACTT 
CACTTGATCT CGCTGGCCCG CATTGCCATC TTCATGATTG CCATTTTGGT GCATGGACGC 
CTGGTGGAGG GCACTGCCCG GCTGGACTTC CTCTGGCAGC TGCAGGCGTC GCAGGAGAAG 
AAGGAGATGG ATGTGCTACA GGAGTCCAAT AAGCGCATTC TACACAATTT GCTGCCCGCT 
CATGTGGCTG CACATTTTCT CGATGCGCAA TTTCGCAACA ACATGGAACT CTACCACCAG 
AGCTATGCCA AAGTGGGCGT CATCTTTGCA AGCGTACCGA ACTTCAACGA GTTTTACACG 
GAAATGGATG GCTCCGATCA GGGTCTTGAG TGCCTCCGTC TGCTCAACGA GATTATTGCG 
GACTTTGATG AGCTGCTCAA GGAGGATCGT TTCCGCGGCA TCGACAAGAT CAAGACCGTT 
GGTAGCACTT ATATGGCTGT CGTGGGACTC ATACCCGAGT ACAAAATCCA GCCTAACGAT 
CCGAACTCAG TGCGGCGCCA CATGACAGCG CTCATTGAGT ATGTCAAGGC CATGAGGCAC 
TCACTCCAAG AGATAAACAG TCACTCGTAC AATAACTTCA TGCTGCGCGT GGGCATCAAC 
ATTGGACCCG TTGTGGCGGG CGTAATTGGA GCTCGAAAGC CACAATATGA CATTTGGGGC 
AATACGGTGA ACGTGGCCAG TCGCATGGAC TCCACCGGAG TTCCCGGCTA CTCGCAAGTA 
ACTCAAGAGG TGGTCGACAG CCTGGTGGGG TCGCACTTTG AATTCCGCTG TCGTGGCACC 
ATTAAGGTGA AGGGCAAAGG CGACATGGTC ACATATTTCT TGTGTGACAG CGGCAACAAA 
TCGCTGAACG GAGAAGTGCG CAACGCCATG TCCCTGCCAC AGAGTCTCCA TGCGCCGGAT 
TACTATATGA AGGTGTCGCA GTTTCCGGAG AATCGCGTCA ATACCGACAC CTATAGCAAA 
AAGGAGAATG GACATCTGTA CGCTGGCAAC GGCGTCGAGG AGCAGCAGTT GTTGCTGCAG 
CATCAGCACA AACAGCACGA TCCGCTACCG CTTCCTGCTC CTCCGCCTCC GGTTCACCAT 
CATTTGCATC AGCAGCAACA GCAGCGTTTG AACAGCAAGC TGCAAAAGCA ACCGATCTTC 
ATGGCCAACG GTGGTCTGCC CAATATCCGG GAGAATGGCA ATGGTCACAA TGGAGAGCAT 
CAGCAGCAGC AGCAGCAGCA GCAGCAACAT CAGCAGCAGC AACAGCAGCA GCAGCAGCAC 
GGTGGCTTTA TGGTGGCAAC GACGACACCG CCGGCTGCAG TGGCGGTTCC CCTCCAGCCC 
CAGCACCATC AACTCCAGTT CCAGCACCCG CACCAGCATC CACTCCCGTC AGCAGTGTCG 
GTTCCAGTGC AGCACCAGAT CCTACTCCAC CACCAGCTCC AGCTCCAGCA CCAGCCCGTT 
CCTTCGGTAA TGTTGCGCGA GTTTAATATT ATTGAGAATC CGACTTCCGG TGGCCGGCAT 
CAGCAGATGG AGCAGCTGCC GCCGCATCAC GGCAGCCTCG ACCTATCGGG AATGGGAATG 
GGTGTGGGGG CTGGCGTGCT CGGCAGCGAC TGCTTCATGA TGCCACGGCG AGATCGCGAA 
CGCACATATG TGCCGCCCTT AAACCAGCAT GGCCATCATC CACCGCATCA CCTCCATTCG 
AATCTGAATC TGAATCAGAG CCAGCACCCG CCATCGTTCA CCAGCCTGGG CTATGGCCAA 
TGCCGGGAGA GCGAGCCGCT ATTGCATGCC AGCAGCGTCG CGCCGGTAGC CAAGCACCAG 
CAGCAGCAGC AGCAGCAGCA TCAGCATCAG CAGCCGCAAT CGCAATCTGC CCAGGATCAG 
CAGACACATC CCGCCCAGGA CCCACATCCT TTGCAGCGGC AGTACGCCAT GTATTCGCAG 
CAGCCGCAGC TGCCGCCGAA GCCAGTGCTG AGAACGTACA TGAAGCCGTT GCCGAAGCTG 
CCGACGGATC TGGAGGAGAG CCGCGACATG TCCTCGACGG ATGATCTGAG CTCACGACCA 
CACTCGCCCT CGATGAGCAG CTCGGATGAG AGCTACTCGA AGACCACAGA GGGCGAAGGT 
GAAGGCGATG AGGACTCGCC GCGCATGGTC AATGGTGGCC ACCTGCATCA TCGCAATGGC 
TACCACCTGC CCGCCGGTGG TCTGGTCAAC CCATTGCAAT GGCTCTACCC CTGCGACATT 
CAGGTGGATC CCACCTCTCC GGTCGTCGAC ATGGCCCACC TGCATGACTT TGAGCTAAGC 
TCCACCACCG AGAGCCAGGG CCATCACACT AACAGCAACA CGACGAGCAA CACACAACAC 
AAGGGCGACA GCTGCAACAG CTTCGACTTT CAGAAGGCAG CAGTTGGAAC GGCAGCGGGT 
GCGGCAATCG CGACCAAGTC GCCCTTCGAG CGTGAACTGC AACGTCTGCT TAACGAATCC 
TCGCGAGCTC GTTGCCTGGC GACTGCGACA ACGACGGCTG GAGCCATCTC TACCACAGAT 
CAGACGGCCA GCAATGGAAG CCGTGAGTTG AGCTACTCCC TCAGCAATGG GAAGCTGAGC 
TCTGCGAATG GACATGGCGT TGGAGGATCC GGATCCGGAT CCGGATCGGG ATCAGGTTCA 
GGTTCGGCGG TGGGCAATGG CAGCGGTGGC AGCGGCAGCA GCAATGGCAA TCTCAGCGGC 
GGCAGCGGCA GCAACTCGAA CAGTGGCAAC AACAACAGCA GTCACCACAA GACGGAGCAG 
CAGCAGAATA TGGATCACGA GCATCTGGCC GGTGGCAAAT TGCTGGGCAG TAATAGCTTC 
ATGATCGCCA AACATCCGGT GGGCTTGGAA GCCATCAAGG AGATAACGCG CAACAAGAAT 
CCCTCGGAGT CCAGCCAAAT GCAAACCTCG GACACGGAGT CCTGCGAGAT TCTGCACGAG 
AACCGCAACC AGATGCATGT GCTGGCCATG CTGGAGATGC ATACGGCCAA GGAGCTGAAT 
GGCAGCCATG CGCATCACGG TCAGCATCAC CAGCAGCCAC AGAGGACGCA TCGCCAGCGA 
CCGCGCTCCA AGGAGCTGCA GTACAGCCAC GAGAGTCTGG ACGGACTGGA TGGAGCGGTG 
CAGTCGCAGT CGCAGCAGCG ACACCAACGC TATCATCACC ACCATCACCA CCAACAGAGG 
CAGCAGCAAC AGCAGCGCTA CAACCATGTG CAGGAGCAGG AGGAGCGTGA CGACACCGAG 
GACAACCTGG CCGATGAGGA GTTTGAGGAC GACGAAGTGG GTCGTGATGT GCGACAGAAG 
CGCCTACAAA AGTCCGAGCT CAATCACAAA CGATCGGAGG TTGCAACAGA GGCAGGCAAT 
CACCACGACG ACGAGGTGGA GGAGGAGGAC GACGATGACG ACGAAGAGGA GGACCACCGG 
AATGGCGGGC GGGAGGCGGC ACCGCTGACT AACGGCAGCA TGCGCGGCCT GGAGGCCAAT 
GTGATAAACG ACGAGCTGAA GTATGGAGCA ACCCATCTCA ATCACCAGTC CATGGACTCC 
AATCCGTTGG AGAGCCAATC GGAGTGGTCG GATGACGATT GTCGTGAGGA AGCAACAGGG 
GGAGCCGAGT CCACGGGCTA CATTACGGAT GAGCCCGGAC TGGAGAACAT CTCGTTGCTG 
AACGAAGCTG GTCTCACCGA TGCCGAGGGC GCTCTCTCGG ATGTCAACTC GCTGTATAAC 
GCGCCGGACG TGGACGACAC TTCGGTGTCC AGCCGGGCCA GCTCCCGCCT GCTTAGCCTG 
GACTCGCTGA GCGGCCTCTA CGACTGCGAT CTGGACTCCA AGCACGAGCT GGCCATCGTC 
AATGCCTCGC ACAAGATCTC CAGTAAATTT GGGCAGCCTC TATCACCGGC ACAGCAGCAA 
CATCAACAAC AGCAGCAACA GCAACAGCAG CAACAGCAGC AACACCACCA ACAGCAGCTG 
CAGCAGAATC CGCAGCACAC TCAAGCCCAA TCGCATTTGG CTCCGGTTCA GTTTCAGTCC 
GCCGAGGAGT TGAGAGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rut ( NM_001272621.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259550.1 Drosophila melanogaster rutabaga (rut), transcript variant E, mRNA.

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CloneID	OFa03566
Clone ID Related Accession (Same CDS sequence)	NM_001272621.2
Accession Version	NM_001272621.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4176bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	rutabaga, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001272621.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATCATG CCGTGAAGGC GACGCGCGGC CGCCCGTTGA ATACGCTGCG CTTCGAGAAC 
GATGAGCTGG AATGCCTCTA CCAGCGGTAT ACGCTCAAGC TGCAGCGCTT CTCGGTGCTG 
GGCGTGGTGG CTCTGGTCTT CGTCCTCTGC GGCGTGATGG CGGCCCTTTC GCTGACCTTC 
AACAACGCGG CCACCTTTCA CAACATCTTC AATGCGATCG TTTGTGGACT GTTTGCCGTG 
GTGCTGGTGC TGCTGCAGTG TTCCGTGATC AAGGACCACC ACCTGCCCAC ATTGTGCTAC 
GGAATCCTGC TATTCACGGC CAGCATCTGT GTGGTGTCGA TGCCCACATT GGGCAGCGTG 
TTCCCGGTGG ACACCAAGGA GGTGATGGCC GAGGGCGTCT GGCAGATCGT GTTCGTGGTC 
TTCCTCGCTT ACGCCATGAT GCCGCTGCAG ATTTGGGAGG CGGTGGCCTT TGGCATCGCC 
CTGCCCTCGG TGCACATAAG TCTAACGGTC TATAAGATTT TCACGGATGC GCTGCGCTAC 
CTGGAGTATA ATCAACTGAT TGCGAACATT GTCATCTTTA TTGGTGTAAA TGTGGCCGGT 
CTCGTGGTGA ACATCATGAT GGAGCGCGCA CAGCGTCGCA CATTTCTCGA TACACGCAAC 
TGCATCGCAT CCCGTCTGGA GATCCAGGAT GAGAACGAGA AACTGGAACG TCTGTTGCTA 
TCGGTGCTGC CGCAGCACGT GGCCATGCAG ATGAAGAACG ACATCCTGTC GCCGGTGGCT 
GGGCAATTTC ATCGCATCTA CATTCAGAAG CACGAGAATG TGAGCATCCT TTTCGCGGAC 
ATTGTCGGAT TCACGGTGCT CTCCTCGCAG TGTAGTGCCC AGGAGCTGGT CCGCCTGCTC 
AACGAGCTCT TTGGCCGCTT CGATCAGCTG GCACACGATA ATCACTGTTT GCGTATCAAG 
ATCCTGGGCG ACTGTTACTA TTGTGTCTCC GGATTGCCAG AACCTCGCAA GGATCACGCC 
AAATGCGCCG TGGAAATGGG ACTCGATATG ATAGATGCCA TTGCCACCGT GGTGGAGGCC 
ACCGACGTCA TCCTGAACAT GCGCGTGGGC ATCCACACGG GCCGTGTGCT CTGCGGGGTC 
TTGGGCCTTC GCAAGTGGCA GTTCGACGTG TGGTCAAACG ATGTGACCCT GGCCAATCAC 
ATGGAGTCCG GCGGGGAACC GGGTCGGGTG CACGTAACCC GCGCCACCCT GGACTCCTTG 
TCCGGCGAAT ACGAGGTGGA GGCTGGCCAC GGCGACGAAC GGTCCTCGTA TCTGCGCGAC 
CATGGTGTGG ACACGTTCTT CATCGTTCCG CCGCCACATC GACGCAAGCC ACTGATGCTC 
AACACACTGG GCGTTCGCTC GGCGATCGGC AGTCGACGGA AGTTGTCGTT CAGAAACGTG 
TCCAATGTGG TCATGCAGCT GCTGCACACG ATCAAGTTCT CGGAACCGGT GCCGTTCTCC 
AATATAGCCA CCGGATCGTT CCCCTCAGCG GCCAGCGCCT TGGGTGGCGG AGTTTCAGTC 
GGCGGTGGTG GAGGAGGAGG AGGAGGAGGC GTGGCACGGG GTAGCACCTG CGAGGCGAAC 
TCTGGAAACG TTCAGGTGTC GGAGAAGGGA TCGCGCAAGT CCCAGTCGAA GGTGGCGGAC 
AAATTCAAGA GGCCATTCCG GAAGCGGCAC TCGGTGGCCG CCCACCATCA GCCAACGAAT 
CGGGTGAATC GATTCCTTTC CCAGGCGATC AATGCCCGGT CGGTGGACTG CGACAAATCG 
GAGCATGTGG ATCGATTGAC CCTGCGATTC CGACAGAGCG ACATGGAACG CGAGTATCAC 
AAGGATTTCG ATCTGGGCTT CACTACGGCC ATGGGTTGCT CCCTGCTGTT GCTCATCCTC 
GGTGCAGCGC TCCAGGTGAC TGCTCTGCCG CGCACTCTCA TCCTGCTGCT GCTCTTTCTC 
TTTGCCTTCA TTTGGGTGAG CGCCATTCTG ATGCTCCTGC TCGCCGTGCG TCTCAAATGG 
ATCATCTGGG ATATTTCGGA GAGCTTTTCG CTGCGCATGG CCATCACGAT TTTCACGGTC 
ATACTCATCT ATTCCGTGGG ACAAGTGAAT GTGTTTACGT GCGTTTCGGA TCATCCATGC 
TCGGGCAATG GGACGACCTC CTTCCAGAAC GACTCTCATC GCAAGTGCTC GCTGCCGCAG 
TACGTGTCCC TATCGGCTGC CTTTGCCTTT CTATCTGTAT CGGTATTCCT GAGGCTGCCC 
ATCATATTCA AATCGCTCCT AGTTCTGGGC ATGGGCACCA TCTATGGCCT TTTCATAGAG 
CTATCGCATC AGAATATCTT TGAGTGCTAC GACAACCGGG TCAACGCCTC GATTCCACTT 
CACTTGATCT CGCTGGCCCG CATTGCCATC TTCATGATTG CCATTTTGGT GCATGGACGC 
CTGGTGGAGG GCACTGCCCG GCTGGACTTC CTCTGGCAGC TGCAGGCGTC GCAGGAGAAG 
AAGGAGATGG ATGTGCTACA GGAGTCCAAT AAGCGCATTC TACACAATTT GCTGCCCGCT 
CATGTGGCTG CACATTTTCT CGATGCGCAA TTTCGCAACA ACATGGAACT CTACCACCAG 
AGCTATGCCA AAGTGGGCGT CATCTTTGCA AGCGTACCGA ACTTCAACGA GTTTTACACG 
GAAATGGATG GCTCCGATCA GGGTCTTGAG TGCCTCCGTC TGCTCAACGA GATTATTGCG 
GACTTTGATG AGCTGCTCAA GGAGGATCGT TTCCGCGGCA TCGACAAGAT CAAGACCGTT 
GGTAGCACTT ATATGGCTGT CGTGGGACTC ATACCCGAGT ACAAAATCCA GCCTAACGAT 
CCGAACTCAG TGCGGCGCCA CATGACAGCG CTCATTGAGT ATGTCAAGGC CATGAGGCAC 
TCACTCCAAG AGATAAACAG TCACTCGTAC AATAACTTCA TGCTGCGCGT GGGCATCAAC 
ATTGGACCCG TTGTGGCGGG CGTAATTGGA GCTCGAAAGC CACAATATGA CATTTGGGGC 
AATACGGTGA ACGTGGCCAG TCGCATGGAC TCCACCGGAG TTCCCGGCTA CTCGCAAGTA 
ACTCAAGAGG TGGTCGACAG CCTGGTGGGG TCGCACTTTG AATTCCGCTG TCGTGGCACC 
ATTAAGGTGA AGGGCAAAGG CGACATGGTC ACATATTTCT TGTGTGACAG CGGCAACAAA 
TCGCTGAACG GAGAAGTGCG CAACGCCATG TCCCTGCCAC AGAGTCTCCA TGCGCCGGAT 
TACTATATGA AGGTGTCGCA GTTTCCGGAG AATCGCGTCA ATACCGACAC CTATAGCAAA 
AAGGAGAATG GACATCTGTA CGCTGGCAAC GGCGTCGAGG AGCAGCAGTT GTTGCTGCAG 
CATCAGCACA AACAGCACGA TCCGCTACCG CTTCCTGCTC CTCCGCCTCC GGTTCACCAT 
CATTTGCATC AGCAGCAACA GCAGCGTTTG AACAGCAAGC TGCAAAAGCA ACCGATCTTC 
ATGGCCAACG GTGGTCTGCC CAATATCCGG GAGAATGGCA ATGGTCACAA TGGAGAGCAT 
CAGCAGCAGC AGCAGCAGCA GCAGCAACAT CAGCAGCAGC AACAGCAGCA GCAGCAGCAC 
GGTGGCTTTA TGGTGGCAAC GACGACACCG CCGGCTGCAG TGGCGGTTCC CCTCCAGCCC 
CAGCACCATC AACTCCAGTT CCAGCACCCG CACCAGCATC CACTCCCGTC AGCAGTGTCG 
GTTCCAGTGC AGCACCAGAT CCTACTCCAC CACCAGCTCC AGCTCCAGCA CCAGCCCGTT 
CCTTCGGCAA ACCTCGGACA CGGAGTCCTG CGAGATTCTG CACGAGAACC GCAACCAGAT 
GCATGTGCTG GCCATGCTGG AGATGCATAC GGCCAAGGAG CTGAATGGCA GCCATGCGCA 
TCACGGTCAG CATCACCAGC AGCCACAGAG GACGCATCGC CAGCGACCGC GCTCCAAGGA 
GCTGCAGTAC AGCCACGAGA GTCTGGACGG ACTGGATGGA GCGGTGCAGT CGCAGTCGCA 
GCAGCGACAC CAACGCTATC ATCACCACCA TCACCACCAA CAGAGGCAGC AGCAACAGCA 
GCGCTACAAC CATGTGCAGG AGCAGGAGGA GCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259550.1
CDS	379..4554
Translation	MDHAVKATRGRPLNTLRFENDELECLYQRYTLKLQRFSVLGVVALVFVLCGVMAALSLTFNNAATFHNIFNAIVCGLFAVVLVLLQCSVIKDHHLPTLCYGILLFTASICVVSMPTLGSVFPVDTKEVMAEGVWQIVFVVFLAYAMMPLQIWEAVAFGIALPSVHISLTVYKIFTDALRYLEYNQLIANIVIFIGVNVAGLVVNIMMERAQRRTFLDTRNCIASRLEIQDENEKLERLLLSVLPQHVAMQMKNDILSPVAGQFHRIYIQKHENVSILFADIVGFTVLSSQCSAQELVRLLNELFGRFDQLAHDNHCLRIKILGDCYYCVSGLPEPRKDHAKCAVEMGLDMIDAIATVVEATDVILNMRVGIHTGRVLCGVLGLRKWQFDVWSNDVTLANHMESGGEPGRVHVTRATLDSLSGEYEVEAGHGDERSSYLRDHGVDTFFIVPPPHRRKPLMLNTLGVRSAIGSRRKLSFRNVSNVVMQLLHTIKFSEPVPFSNIATGSFPSAASALGGGVSVGGGGGGGGGGVARGSTCEANSGNVQVSEKGSRKSQSKVADKFKRPFRKRHSVAAHHQPTNRVNRFLSQAINARSVDCDKSEHVDRLTLRFRQSDMEREYHKDFDLGFTTAMGCSLLLLILGAALQVTALPRTLILLLLFLFAFIWVSAILMLLLAVRLKWIIWDISESFSLRMAITIFTVILIYSVGQVNVFTCVSDHPCSGNGTTSFQNDSHRKCSLPQYVSLSAAFAFLSVSVFLRLPIIFKSLLVLGMGTIYGLFIELSHQNIFECYDNRVNASIPLHLISLARIAIFMIAILVHGRLVEGTARLDFLWQLQASQEKKEMDVLQESNKRILHNLLPAHVAAHFLDAQFRNNMELYHQSYAKVGVIFASVPNFNEFYTEMDGSDQGLECLRLLNEIIADFDELLKEDRFRGIDKIKTVGSTYMAVVGLIPEYKIQPNDPNSVRRHMTALIEYVKAMRHSLQEINSHSYNNFMLRVGINIGPVVAGVIGARKPQYDIWGNTVNVASRMDSTGVPGYSQVTQEVVDSLVGSHFEFRCRGTIKVKGKGDMVTYFLCDSGNKSLNGEVRNAMSLPQSLHAPDYYMKVSQFPENRVNTDTYSKKENGHLYAGNGVEEQQLLLQHQHKQHDPLPLPAPPPPVHHHLHQQQQQRLNSKLQKQPIFMANGGLPNIRENGNGHNGEHQQQQQQQQQHQQQQQQQQQHGGFMVATTTPPAAVAVPLQPQHHQLQFQHPHQHPLPSAVSVPVQHQILLHHQLQLQHQPVPSANLGHGVLRDSAREPQPDACAGHAGDAYGQGAEWQPCASRSASPAATEDASPATALQGAAVQPRESGRTGWSGAVAVAAATPTLSSPPSPPTEAAATAALQPCAGAGGA

Target ORF information:

RefSeq Version	NM_001272621.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster rutabaga (rut), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272621.2

ORF Insert Sequence:

ATGGATCATG CCGTGAAGGC GACGCGCGGC CGCCCGTTGA ATACGCTGCG CTTCGAGAAC 
GATGAGCTGG AATGCCTCTA CCAGCGGTAT ACGCTCAAGC TGCAGCGCTT CTCGGTGCTG 
GGCGTGGTGG CTCTGGTCTT CGTCCTCTGC GGCGTGATGG CGGCCCTTTC GCTGACCTTC 
AACAACGCGG CCACCTTTCA CAACATCTTC AATGCGATCG TTTGTGGACT GTTTGCCGTG 
GTGCTGGTGC TGCTGCAGTG TTCCGTGATC AAGGACCACC ACCTGCCCAC ATTGTGCTAC 
GGAATCCTGC TATTCACGGC CAGCATCTGT GTGGTGTCGA TGCCCACATT GGGCAGCGTG 
TTCCCGGTGG ACACCAAGGA GGTGATGGCC GAGGGCGTCT GGCAGATCGT GTTCGTGGTC 
TTCCTCGCTT ACGCCATGAT GCCGCTGCAG ATTTGGGAGG CGGTGGCCTT TGGCATCGCC 
CTGCCCTCGG TGCACATAAG TCTAACGGTC TATAAGATTT TCACGGATGC GCTGCGCTAC 
CTGGAGTATA ATCAACTGAT TGCGAACATT GTCATCTTTA TTGGTGTAAA TGTGGCCGGT 
CTCGTGGTGA ACATCATGAT GGAGCGCGCA CAGCGTCGCA CATTTCTCGA TACACGCAAC 
TGCATCGCAT CCCGTCTGGA GATCCAGGAT GAGAACGAGA AACTGGAACG TCTGTTGCTA 
TCGGTGCTGC CGCAGCACGT GGCCATGCAG ATGAAGAACG ACATCCTGTC GCCGGTGGCT 
GGGCAATTTC ATCGCATCTA CATTCAGAAG CACGAGAATG TGAGCATCCT TTTCGCGGAC 
ATTGTCGGAT TCACGGTGCT CTCCTCGCAG TGTAGTGCCC AGGAGCTGGT CCGCCTGCTC 
AACGAGCTCT TTGGCCGCTT CGATCAGCTG GCACACGATA ATCACTGTTT GCGTATCAAG 
ATCCTGGGCG ACTGTTACTA TTGTGTCTCC GGATTGCCAG AACCTCGCAA GGATCACGCC 
AAATGCGCCG TGGAAATGGG ACTCGATATG ATAGATGCCA TTGCCACCGT GGTGGAGGCC 
ACCGACGTCA TCCTGAACAT GCGCGTGGGC ATCCACACGG GCCGTGTGCT CTGCGGGGTC 
TTGGGCCTTC GCAAGTGGCA GTTCGACGTG TGGTCAAACG ATGTGACCCT GGCCAATCAC 
ATGGAGTCCG GCGGGGAACC GGGTCGGGTG CACGTAACCC GCGCCACCCT GGACTCCTTG 
TCCGGCGAAT ACGAGGTGGA GGCTGGCCAC GGCGACGAAC GGTCCTCGTA TCTGCGCGAC 
CATGGTGTGG ACACGTTCTT CATCGTTCCG CCGCCACATC GACGCAAGCC ACTGATGCTC 
AACACACTGG GCGTTCGCTC GGCGATCGGC AGTCGACGGA AGTTGTCGTT CAGAAACGTG 
TCCAATGTGG TCATGCAGCT GCTGCACACG ATCAAGTTCT CGGAACCGGT GCCGTTCTCC 
AATATAGCCA CCGGATCGTT CCCCTCAGCG GCCAGCGCCT TGGGTGGCGG AGTTTCAGTC 
GGCGGTGGTG GAGGAGGAGG AGGAGGAGGC GTGGCACGGG GTAGCACCTG CGAGGCGAAC 
TCTGGAAACG TTCAGGTGTC GGAGAAGGGA TCGCGCAAGT CCCAGTCGAA GGTGGCGGAC 
AAATTCAAGA GGCCATTCCG GAAGCGGCAC TCGGTGGCCG CCCACCATCA GCCAACGAAT 
CGGGTGAATC GATTCCTTTC CCAGGCGATC AATGCCCGGT CGGTGGACTG CGACAAATCG 
GAGCATGTGG ATCGATTGAC CCTGCGATTC CGACAGAGCG ACATGGAACG CGAGTATCAC 
AAGGATTTCG ATCTGGGCTT CACTACGGCC ATGGGTTGCT CCCTGCTGTT GCTCATCCTC 
GGTGCAGCGC TCCAGGTGAC TGCTCTGCCG CGCACTCTCA TCCTGCTGCT GCTCTTTCTC 
TTTGCCTTCA TTTGGGTGAG CGCCATTCTG ATGCTCCTGC TCGCCGTGCG TCTCAAATGG 
ATCATCTGGG ATATTTCGGA GAGCTTTTCG CTGCGCATGG CCATCACGAT TTTCACGGTC 
ATACTCATCT ATTCCGTGGG ACAAGTGAAT GTGTTTACGT GCGTTTCGGA TCATCCATGC 
TCGGGCAATG GGACGACCTC CTTCCAGAAC GACTCTCATC GCAAGTGCTC GCTGCCGCAG 
TACGTGTCCC TATCGGCTGC CTTTGCCTTT CTATCTGTAT CGGTATTCCT GAGGCTGCCC 
ATCATATTCA AATCGCTCCT AGTTCTGGGC ATGGGCACCA TCTATGGCCT TTTCATAGAG 
CTATCGCATC AGAATATCTT TGAGTGCTAC GACAACCGGG TCAACGCCTC GATTCCACTT 
CACTTGATCT CGCTGGCCCG CATTGCCATC TTCATGATTG CCATTTTGGT GCATGGACGC 
CTGGTGGAGG GCACTGCCCG GCTGGACTTC CTCTGGCAGC TGCAGGCGTC GCAGGAGAAG 
AAGGAGATGG ATGTGCTACA GGAGTCCAAT AAGCGCATTC TACACAATTT GCTGCCCGCT 
CATGTGGCTG CACATTTTCT CGATGCGCAA TTTCGCAACA ACATGGAACT CTACCACCAG 
AGCTATGCCA AAGTGGGCGT CATCTTTGCA AGCGTACCGA ACTTCAACGA GTTTTACACG 
GAAATGGATG GCTCCGATCA GGGTCTTGAG TGCCTCCGTC TGCTCAACGA GATTATTGCG 
GACTTTGATG AGCTGCTCAA GGAGGATCGT TTCCGCGGCA TCGACAAGAT CAAGACCGTT 
GGTAGCACTT ATATGGCTGT CGTGGGACTC ATACCCGAGT ACAAAATCCA GCCTAACGAT 
CCGAACTCAG TGCGGCGCCA CATGACAGCG CTCATTGAGT ATGTCAAGGC CATGAGGCAC 
TCACTCCAAG AGATAAACAG TCACTCGTAC AATAACTTCA TGCTGCGCGT GGGCATCAAC 
ATTGGACCCG TTGTGGCGGG CGTAATTGGA GCTCGAAAGC CACAATATGA CATTTGGGGC 
AATACGGTGA ACGTGGCCAG TCGCATGGAC TCCACCGGAG TTCCCGGCTA CTCGCAAGTA 
ACTCAAGAGG TGGTCGACAG CCTGGTGGGG TCGCACTTTG AATTCCGCTG TCGTGGCACC 
ATTAAGGTGA AGGGCAAAGG CGACATGGTC ACATATTTCT TGTGTGACAG CGGCAACAAA 
TCGCTGAACG GAGAAGTGCG CAACGCCATG TCCCTGCCAC AGAGTCTCCA TGCGCCGGAT 
TACTATATGA AGGTGTCGCA GTTTCCGGAG AATCGCGTCA ATACCGACAC CTATAGCAAA 
AAGGAGAATG GACATCTGTA CGCTGGCAAC GGCGTCGAGG AGCAGCAGTT GTTGCTGCAG 
CATCAGCACA AACAGCACGA TCCGCTACCG CTTCCTGCTC CTCCGCCTCC GGTTCACCAT 
CATTTGCATC AGCAGCAACA GCAGCGTTTG AACAGCAAGC TGCAAAAGCA ACCGATCTTC 
ATGGCCAACG GTGGTCTGCC CAATATCCGG GAGAATGGCA ATGGTCACAA TGGAGAGCAT 
CAGCAGCAGC AGCAGCAGCA GCAGCAACAT CAGCAGCAGC AACAGCAGCA GCAGCAGCAC 
GGTGGCTTTA TGGTGGCAAC GACGACACCG CCGGCTGCAG TGGCGGTTCC CCTCCAGCCC 
CAGCACCATC AACTCCAGTT CCAGCACCCG CACCAGCATC CACTCCCGTC AGCAGTGTCG 
GTTCCAGTGC AGCACCAGAT CCTACTCCAC CACCAGCTCC AGCTCCAGCA CCAGCCCGTT 
CCTTCGGCAA ACCTCGGACA CGGAGTCCTG CGAGATTCTG CACGAGAACC GCAACCAGAT 
GCATGTGCTG GCCATGCTGG AGATGCATAC GGCCAAGGAG CTGAATGGCA GCCATGCGCA 
TCACGGTCAG CATCACCAGC AGCCACAGAG GACGCATCGC CAGCGACCGC GCTCCAAGGA 
GCTGCAGTAC AGCCACGAGA GTCTGGACGG ACTGGATGGA GCGGTGCAGT CGCAGTCGCA 
GCAGCGACAC CAACGCTATC ATCACCACCA TCACCACCAA CAGAGGCAGC AGCAACAGCA 
GCGCTACAAC CATGTGCAGG AGCAGGAGGA GCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rut ( NM_001272622.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259551.1 Drosophila melanogaster rutabaga (rut), transcript variant F, mRNA.

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CloneID	OFa03567
Clone ID Related Accession (Same CDS sequence)	NM_001272622.2
Accession Version	NM_001272622.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	rutabaga, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001272622.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATCATG CCGTGAAGGC GACGCGCGGC CGCCCGTTGA ATACGCTGCG CTTCGAGAAC 
GATGAGCTGG AATGCCTCTA CCAGCGGTAT ACGCTCAAGC TGCAGCGCTT CTCGGTGCTG 
GGCGTGGTGG CTCTGGTCTT CGTCCTCTGC GGCGTGATGG CGGCCCTTTC GCTGACCTTC 
AACAACGCGG CCACCTTTCA CAACATCTTC AATGCGATCG TTTGTGGACT GTTTGCCGTG 
GTGCTGGTGC TGCTGCAGTG TTCCGTGATC AAGGACCACC ACCTGCCCAC ATTGTGCTAC 
GGAATCCTGC TATTCACGGC CAGCATCTGT GTGGTGTCGA TGCCCACATT GGGCAGCGTG 
TTCCCGGTGG ACACCAAGGA GGTGATGGCC GAGGGCGTCT GGCAGATCGT GTTCGTGGTC 
TTCCTCGCTT ACGCCATGAT GCCGCTGCAG ATTTGGGAGG CGGTGGCCTT TGGCATCGCC 
CTGCCCTCGG TGCACATAAG TCTAACGGTC TATAAGATTT TCACGGATGC GCTGCGCTAC 
CTGGAGTATA ATCAACTGAT TGCGAACATT GTCATCTTTA TTGGTGTAAA TGTGGCCGGT 
CTCGTGGTGA ACATCATGAT GGAGCGCGCA CAGCGTCGCA CATTTCTCGA TACACGCAAC 
TGCATCGCAT CCCGTCTGGA GATCCAGGAT GAGAACGAGA AACTGGAACG TCTGTTGCTA 
TCGGTGCTGC CGCAGCACGT GGCCATGCAG ATGAAGAACG ACATCCTGTC GCCGGTGGCT 
GGGCAATTTC ATCGCATCTA CATTCAGAAG CACGAGAATG TGAGCATCCT TTTCGCGGAC 
ATTGTCGGAT TCACGGTGCT CTCCTCGCAG TGTAGTGCCC AGGAGCTGGT CCGCCTGCTC 
AACGAGCTCT TTGGCCGCTT CGATCAGCTG GCACACGATA ATCACTGTTT GCGTATCAAG 
ATCCTGGGCG ACTGTTACTA TTGTGTCTCC GGATTGCCAG AACCTCGCAA GGATCACGCC 
AAATGCGCCG TGGAAATGGG ACTCGATATG ATAGATGCCA TTGCCACCGT GGTGGAGGCC 
ACCGACGTCA TCCTGAACAT GCGCGTGGGC ATCCACACGG GCCGTGTGCT CTGCGGGGTC 
TTGGGCCTTC GCAAGTGGCA GTTCGACGTG TGGTCAAACG ATGTGACCCT GGCCAATCAC 
ATGGAGTCCG GCGGGGAACC GGGTCGGGTG CACGTAACCC GCGCCACCCT GGACTCCTTG 
TCCGGCGAAT ACGAGGTGGA GGCTGGCCAC GGCGACGAAC GGTCCTCGTA TCTGCGCGAC 
CATGGTGTGG ACACGTTCTT CATCGTTCCG CCGCCACATC GACGCAAGCC ACTGATGCTC 
AACACACTGG GCGTTCGCTC GGCGATCGGC AGTCGACGGA AGTTGTCGTT CAGAAACGTG 
TCCAATGTGG TCATGCAGCT GCTGCACACG ATCAAGTTCT CGGAACCGGT GCCGTTCTCC 
AATATAGCCA CCGGATCGTT CCCCTCAGCG GCCAGCGCCT TGGGTGGCGG AGTTTCAGTC 
GGCGGTGGTG GAGGAGGAGG AGGAGGAGGC GTGGCACGGG GTAGCACCTG CGAGGCGAAC 
TCTGGAAACG TTCAGGTGTC GGAGAAGGGA TCGCGCAAGT CCCAGTCGAA GGTGGCGGAC 
AAATTCAAGA GGCCATTCCG GAAGCGGCAC TCGGTGGCCG CCCACCATCA GCCAACGAAT 
CGGGTGAATC GATTCCTTTC CCAGGCGATC AATGCCCGGT CGGTGGACTG CGACAAATCG 
GAGCATGTGG ATCGATTGAC CCTGCGATTC CGACAGAGCG ACATGGAACG CGAGTATCAC 
AAGGATTTCG ATCTGGGCTT CACTACGGCC ATGGGTTGCT CCCTGCTGTT GCTCATCCTC 
GGTGCAGCGC TCCAGGTGAC TGCTCTGCCG CGCACTCTCA TCCTGCTGCT GCTCTTTCTC 
TTTGCCTTCA TTTGGGTGAG CGCCATTCTG ATGCTCCTGC TCGCCGTGCG TCTCAAATGG 
ATCATCTGGG ATATTTCGGA GAGCTTTTCG CTGCGCATGG CCATCACGAT TTTCACGGTC 
ATACTCATCT ATTCCGTGGG ACAAGTGAAT GTGTTTACGT GCGTTTCGGA TCATCCATGC 
TCGGGCAATG GGACGACCTC CTTCCAGAAC GACTCTCATC GCAAGTGCTC GCTGCCGCAG 
TACGTGTCCC TATCGGCTGC CTTTGCCTTT CTATCTGTAT CGGTATTCCT GAGGCTGCCC 
ATCATATTCA AATCGCTCCT AGTTCTGGGC ATGGGCACCA TCTATGGCCT TTTCATAGAG 
CTATCGCATC AGAATATCTT TGAGTGCTAC GACAACCGGG TCAACGCCTC GATTCCACTT 
CACTTGATCT CGCTGGCCCG CATTGCCATC TTCATGATTG CCATTTTGGT GCATGGACGC 
CTGGTGGAGG GCACTGCCCG GCTGGACTTC CTCTGGCAGC TGCAGGCGTC GCAGGAGAAG 
AAGGAGATGG ATGTGCTACA GGAGTCCAAT AAGCGCATTC TACACAATTT GCTGCCCGCT 
CATGTGGCTG CACATTTTCT CGATGCGCAA TTTCGCAACA ACATGGAACT CTACCACCAG 
AGCTATGCCA AAGTGGGCGT CATCTTTGCA AGCGTACCGA ACTTCAACGA GTTTTACACG 
GAAATGGATG GCTCCGATCA GGGTCTTGAG TGCCTCCGTC TGCTCAACGA GATTATTGCG 
GACTTTGATG AGCTGCTCAA GGAGGATCGT TTCCGCGGCA TCGACAAGAT CAAGACCGTT 
GGTAGCACTT ATATGGCTGT CGTGGGACTC ATACCCGAGT ACAAAATCCA GCCTAACGAT 
CCGAACTCAG TGCGGCGCCA CATGACAGCG CTCATTGAGT ATGTCAAGGC CATGAGGCAC 
TCACTCCAAG AGATAAACAG TCACTCGTAC AATAACTTCA TGCTGCGCGT GGGCATCAAC 
ATTGGACCCG TTGTGGCGGG CGTAATTGGA GCTCGAAAGC CACAATATGA CATTTGGGGC 
AATACGGTGA ACGTGGCCAG TCGCATGGAC TCCACCGGAG TTCCCGGCTA CTCGCAAGTA 
ACTCAAGAGG TGGTCGACAG CCTGGTGGGG TCGCACTTTG AATTCCGCTG TCGTGGCACC 
ATTAAGGTGA AGGGCAAAGG CGACATGGTC ACATATTTCT TGTGTGACAG CGGCAACAAA 
TCGCTGAACG GAGAAGTGCG CAACGCCATG TCCCTGCCAC AGAGTCTCCA TGCGCCGGAT 
TACTATATGA AGGTGTCGCA GTTTCCGGAG AATCGCGTCA ATACCGACAC CTATAGCAAA 
AAGGAGAATG GACATCTGTA CGCTGGCAAC GGCGTCGAGG AGCAGCAGTT GTTGCTGCAG 
CATCAGCACA AACAGCACGA TCCGCTACCG CTTCCTGCTC CTCCGCCTCC GGTTCACCAT 
CATTTGCATC AGCAGCAACA GCAGCGTTTG AACAGCAAGC TGCAAAAGCA ACCGATCTTC 
ATGGCCAACG GTGGTCTGCC CAATATCCGG GAGAATGGCA ATGGTCACAA TGGAGAGCAT 
CAGCAGCAGC AGCAGCAGCA GCAGCAACAT CAGCAGCAGC AACAGCAGCA GCAGCAGCAC 
GGTGGCTTTA TGGTGGCAAC GACGACACCG CCGGCTGCAG TGGCGGTTCC CCTCCAGCCC 
CAGCACCATC AACTCCAGTT CCAGCACCCG CACCAGCATC CACTCCCGTC AGCAGTGTCG 
GTTCCAGTGC AGCACCAGAT CCTACTCCAC CACCAGCTCC AGCTCCAGCA CCAGCCCGTT 
CCTTCGGTAA TGTTGCGCGA GTTTAATATT ATTGAGAATC CGACTTCCGG TGGCCGGCAT 
CAGCAGATGG AGCAGCTGCC GCCGCATCAC GGCAGCCTCG ACCTATCGGG AATGGGAATG 
GGTGTGGGGG CTGGCGTGCT CGGCAGCGAC TGCTTCATGA TGCCACGGCG AGATCGCGAA 
CGCACATATG TGCCGCCCTT AAACCAGCAT GGCCATCATC CACCGCATCA CCTCCATTCG 
AATCTGAATC TGAATCAGAG CCAGCACCCG CCATCGTTCA CCAGCCTGGG CTATGGCCAA 
TGCCGGGAGA GCGAGCCGCT ATTGCATGCC AGCAGCGTCG CGCCGGTAGC CAAGGCAAAC 
CTCGGACACG GAGTCCTGCG AGATTCTGCA CGAGAACCGC AACCAGATGC ATGTGCTGGC 
CATGCTGGAG ATGCATACGG CCAAGGAGCT GAATGGCAGC CATGCGCATC ACGGTCAGCA 
TCACCAGCAG CCACAGAGGA CGCATCGCCA GCGACCGCGC TCCAAGGAGC TGCAGTACAG 
CCACGAGAGT CTGGACGGAC TGGATGGAGC GGTGCAGTCG CAGTCGCAGC AGCGACACCA 
ACGCTATCAT CACCACCATC ACCACCAACA GAGGCAGCAG CAACAGCAGC GCTACAACCA 
TGTGCAGGAG CAGGAGGAGC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259551.1
CDS	379..4902
Translation	MDHAVKATRGRPLNTLRFENDELECLYQRYTLKLQRFSVLGVVALVFVLCGVMAALSLTFNNAATFHNIFNAIVCGLFAVVLVLLQCSVIKDHHLPTLCYGILLFTASICVVSMPTLGSVFPVDTKEVMAEGVWQIVFVVFLAYAMMPLQIWEAVAFGIALPSVHISLTVYKIFTDALRYLEYNQLIANIVIFIGVNVAGLVVNIMMERAQRRTFLDTRNCIASRLEIQDENEKLERLLLSVLPQHVAMQMKNDILSPVAGQFHRIYIQKHENVSILFADIVGFTVLSSQCSAQELVRLLNELFGRFDQLAHDNHCLRIKILGDCYYCVSGLPEPRKDHAKCAVEMGLDMIDAIATVVEATDVILNMRVGIHTGRVLCGVLGLRKWQFDVWSNDVTLANHMESGGEPGRVHVTRATLDSLSGEYEVEAGHGDERSSYLRDHGVDTFFIVPPPHRRKPLMLNTLGVRSAIGSRRKLSFRNVSNVVMQLLHTIKFSEPVPFSNIATGSFPSAASALGGGVSVGGGGGGGGGGVARGSTCEANSGNVQVSEKGSRKSQSKVADKFKRPFRKRHSVAAHHQPTNRVNRFLSQAINARSVDCDKSEHVDRLTLRFRQSDMEREYHKDFDLGFTTAMGCSLLLLILGAALQVTALPRTLILLLLFLFAFIWVSAILMLLLAVRLKWIIWDISESFSLRMAITIFTVILIYSVGQVNVFTCVSDHPCSGNGTTSFQNDSHRKCSLPQYVSLSAAFAFLSVSVFLRLPIIFKSLLVLGMGTIYGLFIELSHQNIFECYDNRVNASIPLHLISLARIAIFMIAILVHGRLVEGTARLDFLWQLQASQEKKEMDVLQESNKRILHNLLPAHVAAHFLDAQFRNNMELYHQSYAKVGVIFASVPNFNEFYTEMDGSDQGLECLRLLNEIIADFDELLKEDRFRGIDKIKTVGSTYMAVVGLIPEYKIQPNDPNSVRRHMTALIEYVKAMRHSLQEINSHSYNNFMLRVGINIGPVVAGVIGARKPQYDIWGNTVNVASRMDSTGVPGYSQVTQEVVDSLVGSHFEFRCRGTIKVKGKGDMVTYFLCDSGNKSLNGEVRNAMSLPQSLHAPDYYMKVSQFPENRVNTDTYSKKENGHLYAGNGVEEQQLLLQHQHKQHDPLPLPAPPPPVHHHLHQQQQQRLNSKLQKQPIFMANGGLPNIRENGNGHNGEHQQQQQQQQQHQQQQQQQQQHGGFMVATTTPPAAVAVPLQPQHHQLQFQHPHQHPLPSAVSVPVQHQILLHHQLQLQHQPVPSVMLREFNIIENPTSGGRHQQMEQLPPHHGSLDLSGMGMGVGAGVLGSDCFMMPRRDRERTYVPPLNQHGHHPPHHLHSNLNLNQSQHPPSFTSLGYGQCRESEPLLHASSVAPVAKANLGHGVLRDSAREPQPDACAGHAGDAYGQGAEWQPCASRSASPAATEDASPATALQGAAVQPRESGRTGWSGAVAVAAATPTLSSPPSPPTEAAATAALQPCAGAGGA

Target ORF information:

RefSeq Version	NM_001272622.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster rutabaga (rut), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272622.2

ORF Insert Sequence:

ATGGATCATG CCGTGAAGGC GACGCGCGGC CGCCCGTTGA ATACGCTGCG CTTCGAGAAC 
GATGAGCTGG AATGCCTCTA CCAGCGGTAT ACGCTCAAGC TGCAGCGCTT CTCGGTGCTG 
GGCGTGGTGG CTCTGGTCTT CGTCCTCTGC GGCGTGATGG CGGCCCTTTC GCTGACCTTC 
AACAACGCGG CCACCTTTCA CAACATCTTC AATGCGATCG TTTGTGGACT GTTTGCCGTG 
GTGCTGGTGC TGCTGCAGTG TTCCGTGATC AAGGACCACC ACCTGCCCAC ATTGTGCTAC 
GGAATCCTGC TATTCACGGC CAGCATCTGT GTGGTGTCGA TGCCCACATT GGGCAGCGTG 
TTCCCGGTGG ACACCAAGGA GGTGATGGCC GAGGGCGTCT GGCAGATCGT GTTCGTGGTC 
TTCCTCGCTT ACGCCATGAT GCCGCTGCAG ATTTGGGAGG CGGTGGCCTT TGGCATCGCC 
CTGCCCTCGG TGCACATAAG TCTAACGGTC TATAAGATTT TCACGGATGC GCTGCGCTAC 
CTGGAGTATA ATCAACTGAT TGCGAACATT GTCATCTTTA TTGGTGTAAA TGTGGCCGGT 
CTCGTGGTGA ACATCATGAT GGAGCGCGCA CAGCGTCGCA CATTTCTCGA TACACGCAAC 
TGCATCGCAT CCCGTCTGGA GATCCAGGAT GAGAACGAGA AACTGGAACG TCTGTTGCTA 
TCGGTGCTGC CGCAGCACGT GGCCATGCAG ATGAAGAACG ACATCCTGTC GCCGGTGGCT 
GGGCAATTTC ATCGCATCTA CATTCAGAAG CACGAGAATG TGAGCATCCT TTTCGCGGAC 
ATTGTCGGAT TCACGGTGCT CTCCTCGCAG TGTAGTGCCC AGGAGCTGGT CCGCCTGCTC 
AACGAGCTCT TTGGCCGCTT CGATCAGCTG GCACACGATA ATCACTGTTT GCGTATCAAG 
ATCCTGGGCG ACTGTTACTA TTGTGTCTCC GGATTGCCAG AACCTCGCAA GGATCACGCC 
AAATGCGCCG TGGAAATGGG ACTCGATATG ATAGATGCCA TTGCCACCGT GGTGGAGGCC 
ACCGACGTCA TCCTGAACAT GCGCGTGGGC ATCCACACGG GCCGTGTGCT CTGCGGGGTC 
TTGGGCCTTC GCAAGTGGCA GTTCGACGTG TGGTCAAACG ATGTGACCCT GGCCAATCAC 
ATGGAGTCCG GCGGGGAACC GGGTCGGGTG CACGTAACCC GCGCCACCCT GGACTCCTTG 
TCCGGCGAAT ACGAGGTGGA GGCTGGCCAC GGCGACGAAC GGTCCTCGTA TCTGCGCGAC 
CATGGTGTGG ACACGTTCTT CATCGTTCCG CCGCCACATC GACGCAAGCC ACTGATGCTC 
AACACACTGG GCGTTCGCTC GGCGATCGGC AGTCGACGGA AGTTGTCGTT CAGAAACGTG 
TCCAATGTGG TCATGCAGCT GCTGCACACG ATCAAGTTCT CGGAACCGGT GCCGTTCTCC 
AATATAGCCA CCGGATCGTT CCCCTCAGCG GCCAGCGCCT TGGGTGGCGG AGTTTCAGTC 
GGCGGTGGTG GAGGAGGAGG AGGAGGAGGC GTGGCACGGG GTAGCACCTG CGAGGCGAAC 
TCTGGAAACG TTCAGGTGTC GGAGAAGGGA TCGCGCAAGT CCCAGTCGAA GGTGGCGGAC 
AAATTCAAGA GGCCATTCCG GAAGCGGCAC TCGGTGGCCG CCCACCATCA GCCAACGAAT 
CGGGTGAATC GATTCCTTTC CCAGGCGATC AATGCCCGGT CGGTGGACTG CGACAAATCG 
GAGCATGTGG ATCGATTGAC CCTGCGATTC CGACAGAGCG ACATGGAACG CGAGTATCAC 
AAGGATTTCG ATCTGGGCTT CACTACGGCC ATGGGTTGCT CCCTGCTGTT GCTCATCCTC 
GGTGCAGCGC TCCAGGTGAC TGCTCTGCCG CGCACTCTCA TCCTGCTGCT GCTCTTTCTC 
TTTGCCTTCA TTTGGGTGAG CGCCATTCTG ATGCTCCTGC TCGCCGTGCG TCTCAAATGG 
ATCATCTGGG ATATTTCGGA GAGCTTTTCG CTGCGCATGG CCATCACGAT TTTCACGGTC 
ATACTCATCT ATTCCGTGGG ACAAGTGAAT GTGTTTACGT GCGTTTCGGA TCATCCATGC 
TCGGGCAATG GGACGACCTC CTTCCAGAAC GACTCTCATC GCAAGTGCTC GCTGCCGCAG 
TACGTGTCCC TATCGGCTGC CTTTGCCTTT CTATCTGTAT CGGTATTCCT GAGGCTGCCC 
ATCATATTCA AATCGCTCCT AGTTCTGGGC ATGGGCACCA TCTATGGCCT TTTCATAGAG 
CTATCGCATC AGAATATCTT TGAGTGCTAC GACAACCGGG TCAACGCCTC GATTCCACTT 
CACTTGATCT CGCTGGCCCG CATTGCCATC TTCATGATTG CCATTTTGGT GCATGGACGC 
CTGGTGGAGG GCACTGCCCG GCTGGACTTC CTCTGGCAGC TGCAGGCGTC GCAGGAGAAG 
AAGGAGATGG ATGTGCTACA GGAGTCCAAT AAGCGCATTC TACACAATTT GCTGCCCGCT 
CATGTGGCTG CACATTTTCT CGATGCGCAA TTTCGCAACA ACATGGAACT CTACCACCAG 
AGCTATGCCA AAGTGGGCGT CATCTTTGCA AGCGTACCGA ACTTCAACGA GTTTTACACG 
GAAATGGATG GCTCCGATCA GGGTCTTGAG TGCCTCCGTC TGCTCAACGA GATTATTGCG 
GACTTTGATG AGCTGCTCAA GGAGGATCGT TTCCGCGGCA TCGACAAGAT CAAGACCGTT 
GGTAGCACTT ATATGGCTGT CGTGGGACTC ATACCCGAGT ACAAAATCCA GCCTAACGAT 
CCGAACTCAG TGCGGCGCCA CATGACAGCG CTCATTGAGT ATGTCAAGGC CATGAGGCAC 
TCACTCCAAG AGATAAACAG TCACTCGTAC AATAACTTCA TGCTGCGCGT GGGCATCAAC 
ATTGGACCCG TTGTGGCGGG CGTAATTGGA GCTCGAAAGC CACAATATGA CATTTGGGGC 
AATACGGTGA ACGTGGCCAG TCGCATGGAC TCCACCGGAG TTCCCGGCTA CTCGCAAGTA 
ACTCAAGAGG TGGTCGACAG CCTGGTGGGG TCGCACTTTG AATTCCGCTG TCGTGGCACC 
ATTAAGGTGA AGGGCAAAGG CGACATGGTC ACATATTTCT TGTGTGACAG CGGCAACAAA 
TCGCTGAACG GAGAAGTGCG CAACGCCATG TCCCTGCCAC AGAGTCTCCA TGCGCCGGAT 
TACTATATGA AGGTGTCGCA GTTTCCGGAG AATCGCGTCA ATACCGACAC CTATAGCAAA 
AAGGAGAATG GACATCTGTA CGCTGGCAAC GGCGTCGAGG AGCAGCAGTT GTTGCTGCAG 
CATCAGCACA AACAGCACGA TCCGCTACCG CTTCCTGCTC CTCCGCCTCC GGTTCACCAT 
CATTTGCATC AGCAGCAACA GCAGCGTTTG AACAGCAAGC TGCAAAAGCA ACCGATCTTC 
ATGGCCAACG GTGGTCTGCC CAATATCCGG GAGAATGGCA ATGGTCACAA TGGAGAGCAT 
CAGCAGCAGC AGCAGCAGCA GCAGCAACAT CAGCAGCAGC AACAGCAGCA GCAGCAGCAC 
GGTGGCTTTA TGGTGGCAAC GACGACACCG CCGGCTGCAG TGGCGGTTCC CCTCCAGCCC 
CAGCACCATC AACTCCAGTT CCAGCACCCG CACCAGCATC CACTCCCGTC AGCAGTGTCG 
GTTCCAGTGC AGCACCAGAT CCTACTCCAC CACCAGCTCC AGCTCCAGCA CCAGCCCGTT 
CCTTCGGTAA TGTTGCGCGA GTTTAATATT ATTGAGAATC CGACTTCCGG TGGCCGGCAT 
CAGCAGATGG AGCAGCTGCC GCCGCATCAC GGCAGCCTCG ACCTATCGGG AATGGGAATG 
GGTGTGGGGG CTGGCGTGCT CGGCAGCGAC TGCTTCATGA TGCCACGGCG AGATCGCGAA 
CGCACATATG TGCCGCCCTT AAACCAGCAT GGCCATCATC CACCGCATCA CCTCCATTCG 
AATCTGAATC TGAATCAGAG CCAGCACCCG CCATCGTTCA CCAGCCTGGG CTATGGCCAA 
TGCCGGGAGA GCGAGCCGCT ATTGCATGCC AGCAGCGTCG CGCCGGTAGC CAAGGCAAAC 
CTCGGACACG GAGTCCTGCG AGATTCTGCA CGAGAACCGC AACCAGATGC ATGTGCTGGC 
CATGCTGGAG ATGCATACGG CCAAGGAGCT GAATGGCAGC CATGCGCATC ACGGTCAGCA 
TCACCAGCAG CCACAGAGGA CGCATCGCCA GCGACCGCGC TCCAAGGAGC TGCAGTACAG 
CCACGAGAGT CTGGACGGAC TGGATGGAGC GGTGCAGTCG CAGTCGCAGC AGCGACACCA 
ACGCTATCAT CACCACCATC ACCACCAACA GAGGCAGCAG CAACAGCAGC GCTACAACCA 
TGTGCAGGAG CAGGAGGAGC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rut ( NM_001298317.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285246.1 Drosophila melanogaster rutabaga (rut), transcript variant G, mRNA.

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CloneID	OFa03568
Clone ID Related Accession (Same CDS sequence)	NM_001298317.1 , NM_001272620.2
Accession Version	NM_001298317.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	rutabaga, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATCATG CCGTGAAGGC GACGCGCGGC CGCCCGTTGA ATACGCTGCG CTTCGAGAAC 
GATGAGCTGG AATGCCTCTA CCAGCGGTAT ACGCTCAAGC TGCAGCGCTT CTCGGTGCTG 
GGCGTGGTGG CTCTGGTCTT CGTCCTCTGC GGCGTGATGG CGGCCCTTTC GCTGACCTTC 
AACAACGCGG CCACCTTTCA CAACATCTTC AATGCGATCG TTTGTGGACT GTTTGCCGTG 
GTGCTGGTGC TGCTGCAGTG TTCCGTGATC AAGGACCACC ACCTGCCCAC ATTGTGCTAC 
GGAATCCTGC TATTCACGGC CAGCATCTGT GTGGTGTCGA TGCCCACATT GGGCAGCGTG 
TTCCCGGTGG ACACCAAGGA GGTGATGGCC GAGGGCGTCT GGCAGATCGT GTTCGTGGTC 
TTCCTCGCTT ACGCCATGAT GCCGCTGCAG ATTTGGGAGG CGGTGGCCTT TGGCATCGCC 
CTGCCCTCGG TGCACATAAG TCTAACGGTC TATAAGATTT TCACGGATGC GCTGCGCTAC 
CTGGAGTATA ATCAACTGAT TGCGAACATT GTCATCTTTA TTGGTGTAAA TGTGGCCGGT 
CTCGTGGTGA ACATCATGAT GGAGCGCGCA CAGCGTCGCA CATTTCTCGA TACACGCAAC 
TGCATCGCAT CCCGTCTGGA GATCCAGGAT GAGAACGAGA AACTGGAACG TCTGTTGCTA 
TCGGTGCTGC CGCAGCACGT GGCCATGCAG ATGAAGAACG ACATCCTGTC GCCGGTGGCT 
GGGCAATTTC ATCGCATCTA CATTCAGAAG CACGAGAATG TGAGCATCCT TTTCGCGGAC 
ATTGTCGGAT TCACGGTGCT CTCCTCGCAG TGTAGTGCCC AGGAGCTGGT CCGCCTGCTC 
AACGAGCTCT TTGGCCGCTT CGATCAGCTG GCACACGATA ATCACTGTTT GCGTATCAAG 
ATCCTGGGCG ACTGTTACTA TTGTGTCTCC GGATTGCCAG AACCTCGCAA GGATCACGCC 
AAATGCGCCG TGGAAATGGG ACTCGATATG ATAGATGCCA TTGCCACCGT GGTGGAGGCC 
ACCGACGTCA TCCTGAACAT GCGCGTGGGC ATCCACACGG GCCGTGTGCT CTGCGGGGTC 
TTGGGCCTTC GCAAGTGGCA GTTCGACGTG TGGTCAAACG ATGTGACCCT GGCCAATCAC 
ATGGAGTCCG GCGGGGAACC GGGTCGGGTG CACGTAACCC GCGCCACCCT GGACTCCTTG 
TCCGGCGAAT ACGAGGTGGA GGCTGGCCAC GGCGACGAAC GGTCCTCGTA TCTGCGCGAC 
CATGGTGTGG ACACGTTCTT CATCGTTCCG CCGCCACATC GACGCAAGCC ACTGATGCTC 
AACACACTGG GCGTTCGCTC GGCGATCGGC AGTCGACGGA AGTTGTCGTT CAGAAACGTG 
TCCAATGTGG TCATGCAGCT GCTGCACACG ATCAAGTTCT CGGAACCGGT GCCGTTCTCC 
AATATAGCCA CCGGATCGTT CCCCTCAGCG GCCAGCGCCT TGGGTGGCGG AGTTTCAGTC 
GGCGGTGGTG GAGGAGGAGG AGGAGGAGGC GTGGCACGGG GTAGCACCTG CGAGGCGAAC 
TCTGGAAACG TTCAGGTGTC GGAGAAGGGA TCGCGCAAGT CCCAGTCGAA GGTGGCGGAC 
AAATTCAAGA GGCCATTCCG GAAGCGGCAC TCGGTGGCCG CCCACCATCA GCCAACGAAT 
CGGGTGAATC GATTCCTTTC CCAGGCGATC AATGCCCGGT CGGTGGACTG CGACAAATCG 
GAGCATGTGG ATCGATTGAC CCTGCGATTC CGACAGAGCG ACATGGAACG CGAGTATCAC 
AAGGATTTCG ATCTGGGCTT CACTACGGCC ATGGGTTGCT CCCTGCTGTT GCTCATCCTC 
GGTGCAGCGC TCCAGGTGAC TGCTCTGCCG CGCACTCTCA TCCTGCTGCT GCTCTTTCTC 
TTTGCCTTCA TTTGGGTGAG CGCCATTCTG ATGCTCCTGC TCGCCGTGCG TCTCAAATGG 
ATCATCTGGG ATATTTCGGA GAGCTTTTCG CTGCGCATGG CCATCACGAT TTTCACGGTC 
ATACTCATCT ATTCCGTGGG ACAAGTGAAT GTGTTTACGT GCGTTTCGGA TCATCCATGC 
TCGGGCAATG GGACGACCTC CTTCCAGAAC GACTCTCATC GCAAGTGCTC GCTGCCGCAG 
TACGTGTCCC TATCGGCTGC CTTTGCCTTT CTATCTGTAT CGGTATTCCT GAGGCTGCCC 
ATCATATTCA AATCGCTCCT AGTTCTGGGC ATGGGCACCA TCTATGGCCT TTTCATAGAG 
CTATCGCATC AGAATATCTT TGAGTGCTAC GACAACCGGG TCAACGCCTC GATTCCACTT 
CACTTGATCT CGCTGGCCCG CATTGCCATC TTCATGATTG CCATTTTGGT GCATGGACGC 
CTGGTGGAGG GCACTGCCCG GCTGGACTTC CTCTGGCAGC TGCAGGCGTC GCAGGAGAAG 
AAGGAGATGG ATGTGCTACA GGAGTCCAAT AAGCGCATTC TACACAATTT GCTGCCCGCT 
CATGTGGCTG CACATTTTCT CGATGCGCAA TTTCGCAACA ACATGGAACT CTACCACCAG 
AGCTATGCCA AAGTGGGCGT CATCTTTGCA AGCGTACCGA ACTTCAACGA GTTTTACACG 
GAAATGGATG GCTCCGATCA GGGTCTTGAG TGCCTCCGTC TGCTCAACGA GATTATTGCG 
GACTTTGATG AGCTGCTCAA GGAGGATCGT TTCCGCGGCA TCGACAAGAT CAAGACCGTT 
GGTAGCACTT ATATGGCTGT CGTGGGACTC ATACCCGAGT ACAAAATCCA GCCTAACGAT 
CCGAACTCAG TGCGGCGCCA CATGACAGCG CTCATTGAGT ATGTCAAGGC CATGAGGCAC 
TCACTCCAAG AGATAAACAG TCACTCGTAC AATAACTTCA TGCTGCGCGT GGGCATCAAC 
ATTGGACCCG TTGTGGCGGG CGTAATTGGA GCTCGAAAGC CACAATATGA CATTTGGGGC 
AATACGGTGA ACGTGGCCAG TCGCATGGAC TCCACCGGAG TTCCCGGCTA CTCGCAAGTA 
ACTCAAGAGG TGGTCGACAG CCTGGTGGGG TCGCACTTTG AATTCCGCTG TCGTGGCACC 
ATTAAGGTGA AGGGCAAAGG CGACATGGTC ACATATTTCT TGTGTGACAG CGGCAACAAA 
TCGCTGAACG GAGAAGTGCG CAACGCCATG TCCCTGCCAC AGAGTCTCCA TGCGCCGGAT 
TACTATATGA AGGTGTCGCA GTTTCCGGAG AATCGCGTCA ATACCGACAC CTATAGCAAA 
AAGGAGAATG GACATCTGTA CGCTGGCAAC GGCGTCGAGG AGCAGCAGTT GTTGCTGCAG 
CATCAGCACA AACAGCACGA TCCGCTACCG CTTCCTGCTC CTCCGCCTCC GGTTCACCAT 
CATTTGCATC AGCAGCAACA GCAGCGTTTG AACAGCAAGC TGCAAAAGCA ACCGATCTTC 
ATGGCCAACG GTGGTCTGCC CAATATCCGG GAGAATGGCA ATGGTCACAA TGGAGAGCAT 
CAGCAGCAGC AGCAGCAGCA GCAGCAACAT CAGCAGCAGC AACAGCAGCA GCAGCAGCAC 
GGTGGCTTTA TGGTGGCAAC GACGACACCG CCGGCTGCAG TGGCGGTTCC CCTCCAGCCC 
CAGCACCATC AACTCCAGTT CCAGCACCCG CACCAGCATC CACTCCCGTC AGCAGTGTCG 
GTTCCAGTGC AGCACCAGAT CCTACTCCAC CACCAGCTCC AGCTCCAGCA CCAGCCCGTT 
CCTTCGGTAA TGTTGCGCGA GTTTAATATT ATTGAGAATC CGACTTCCGG TGGCCGGCAT 
CAGCAGATGG AGCAGCTGCC GCCGCATCAC GGCAGCCTCG ACCTATCGGG AATGGGAATG 
GGTGTGGGGG CTGGCGTGCT CGGCAGCGAC TGCTTCATGA TGCCACGGCG AGATCGCGAA 
CGCACATATG TGCCGCCCTT AAACCAGCAT GGCCATCATC CACCGCATCA CCTCCATTCG 
AATCTGAATC TGAATCAGAG CCAGCACCCG CCATCGTTCA CCAGCCTGGG CTATGGCCAA 
TGCCGGGAGA GCGAGCCGCT ATTGCATGCC AGCAGCGTCG CGCCGGTAGC CAAGATCATG 
CCCATGCAGC ATGCGCCAAA GTACGAGCCA CCACGTTACA CTTCTCCCCA CACCATGTTG 
TCGCAACAGC ACCAGCAGCA GCAGCAGCAG CAGCATCAGC ATCAGCAGCC GCAATCGCAA 
TCTGCCCAGG ATCAGCAGAC ACATCCCGCC CAGGACCCAC ATCCTTTGCA GCGGCAGTAC 
GCCATGTATT CGCAGCAGCC GCAGCTGCCG CCGAAGCCAG TGCTGAGAAC GTACATGAAG 
CCGTTGCCGA AGCTGCCGAC GGATCTGGAG GAGAGCCGCG ACATGTCCTC GACGGATGAT 
CTGAGCTCAC GACCACACTC GCCCTCGATG AGCAGCTCGG ATGAGAGCTA CTCGAAGACC 
ACAGAGGGCG AAGGTGAAGG CGATGAGGAC TCGCCGCGCA TGGTCAATGG TGGCCACCTG 
CATCATCGCA ATGGCTACCA CCTGCCCGCC GGTGGTCTGG TCAACCCATT GCAATGGCTC 
TACCCCTGCG ACATTCAGGT GGATCCCACC TCTCCGGTCG TCGACATGGC CCACCTGCAT 
GACTTTGAGC TAAGCTCCAC CACCGAGAGC CAGGGCCATC ACACTAACAG CAACACGACG 
AGCAACACAC AACACAAGGG CGACAGCTGC AACAGCTTCG ACTTTCAGAA GGCAGCAGTT 
GGAACGGCAG CGGGTGCGGC AATCGCGACC AAGTCGCCCT TCGAGCGTGA ACTGCAACGT 
CTGCTTAACG AATCCTCGCG AGCTCGTTGC CTGGCGACTG CGACAACGAC GGCTGGAGCC 
ATCTCTACCA CAGATCAGAC GGCCAGCAAT GGAAGCCGTG AGTTGAGCTA CTCCCTCAGC 
AATGGGAAGC TGAGCTCTGC GAATGGACAT GGCGTTGGAG GATCCGGATC CGGATCCGGA 
TCGGGATCAG GTTCAGGTTC GGCGGTGGGC AATGGCAGCG GTGGCAGCGG CAGCAGCAAT 
GGCAATCTCA GCGGCGGCAG CGGCAGCAAC TCGAACAGTG GCAACAACAA CAGCAGTCAC 
CACAAGACGG AGCAGCAGCA GAATATGGAT CACGAGCATC TGGCCGGTGG CAAATTGCTG 
GGCAGTAATA GCTTCATGAT CGCCAAACAT CCGGTGGGCT TGGAAGCCAT CAAGGAGATA 
ACGCGCAACA AGAATCCCTC GGAGTCCAGC CAAATGCAAA CCTCGGACAC GGAGTCCTGC 
GAGATTCTGC ACGAGAACCG CAACCAGATG CATGTGCTGG CCATGCTGGA GATGCATACG 
GCCAAGGAGC TGAATGGCAG CCATGCGCAT CACGGTCAGC ATCACCAGCA GCCACAGAGG 
ACGCATCGCC AGCGACCGCG CTCCAAGGAG CTGCAGTACA GCCACGAGAG TCTGGACGGA 
CTGGATGGAG CGGTGCAGTC GCAGTCGCAG CAGCGACACC AACGCTATCA TCACCACCAT 
CACCACCAAC AGAGGCAGCA GCAACAGCAG CGCTACAACC ATGTGCAGGA GCAGGAGGAG 
CGTGACGACA CCGAGGACAA CCTGGCCGAT GAGGAGTTTG AGGACGACGA AGTGGGTCGT 
GATGTGCGAC AGAAGCGCCT ACAAAAGTCC GAGCTCAATC ACAAACGATC GGAGGTTGCA 
ACAGAGGCAG GCAATCACCA CGACGACGAG GTGGAGGAGG AGGACGACGA TGACGACGAA 
GAGGAGGACC ACCGGAATGG CGGGCGGGAG GCGGCACCGC TGACTAACGG CAGCATGCGC 
GGCCTGGAGG CCAATGTGAT AAACGACGAG CTGAAGTATG GAGCAACCCA TCTCAATCAC 
CAGTCCATGG ACTCCAATCC GTTGGAGAGC CAATCGGAGT GGTCGGATGA CGATTGTCGT 
GAGGAAGCAA CAGGGGGAGC CGAGTCCACG GGCTACATTA CGGATGAGCC CGGACTGGAG 
AACATCTCGT TGCTGAACGA AGCTGGTCTC ACCGATGCCG AGGGCGCTCT CTCGGATGTC 
AACTCGCTGT ATAACGCGCC GGACGTGGAC GACACTTCGG TGTCCAGCCG GGCCAGCTCC 
CGCCTGCTTA GCCTGGACTC GCTGAGCGGC CTCTACGACT GCGATCTGGA CTCCAAGCAC 
GAGCTGGCCA TCGTCAATGC CTCGCACAAG ATCTCCAGTA AATTTGGGCA GCCTCTATCA 
CCGGCACAGC AGCAACATCA ACAACAGCAG CAACAGCAAC AGCAGCAACA GCAGCAACAC 
CACCAACAGC AGCTGCAGCA GAATCCGCAG CACACTCAAG CCCAATCGCA TTTGGCTCCG 
GTTCAGTTTC AGTCCGCCGA GGAGTTGAGA GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285246.1
CDS	331..6846
Translation	MDHAVKATRGRPLNTLRFENDELECLYQRYTLKLQRFSVLGVVALVFVLCGVMAALSLTFNNAATFHNIFNAIVCGLFAVVLVLLQCSVIKDHHLPTLCYGILLFTASICVVSMPTLGSVFPVDTKEVMAEGVWQIVFVVFLAYAMMPLQIWEAVAFGIALPSVHISLTVYKIFTDALRYLEYNQLIANIVIFIGVNVAGLVVNIMMERAQRRTFLDTRNCIASRLEIQDENEKLERLLLSVLPQHVAMQMKNDILSPVAGQFHRIYIQKHENVSILFADIVGFTVLSSQCSAQELVRLLNELFGRFDQLAHDNHCLRIKILGDCYYCVSGLPEPRKDHAKCAVEMGLDMIDAIATVVEATDVILNMRVGIHTGRVLCGVLGLRKWQFDVWSNDVTLANHMESGGEPGRVHVTRATLDSLSGEYEVEAGHGDERSSYLRDHGVDTFFIVPPPHRRKPLMLNTLGVRSAIGSRRKLSFRNVSNVVMQLLHTIKFSEPVPFSNIATGSFPSAASALGGGVSVGGGGGGGGGGVARGSTCEANSGNVQVSEKGSRKSQSKVADKFKRPFRKRHSVAAHHQPTNRVNRFLSQAINARSVDCDKSEHVDRLTLRFRQSDMEREYHKDFDLGFTTAMGCSLLLLILGAALQVTALPRTLILLLLFLFAFIWVSAILMLLLAVRLKWIIWDISESFSLRMAITIFTVILIYSVGQVNVFTCVSDHPCSGNGTTSFQNDSHRKCSLPQYVSLSAAFAFLSVSVFLRLPIIFKSLLVLGMGTIYGLFIELSHQNIFECYDNRVNASIPLHLISLARIAIFMIAILVHGRLVEGTARLDFLWQLQASQEKKEMDVLQESNKRILHNLLPAHVAAHFLDAQFRNNMELYHQSYAKVGVIFASVPNFNEFYTEMDGSDQGLECLRLLNEIIADFDELLKEDRFRGIDKIKTVGSTYMAVVGLIPEYKIQPNDPNSVRRHMTALIEYVKAMRHSLQEINSHSYNNFMLRVGINIGPVVAGVIGARKPQYDIWGNTVNVASRMDSTGVPGYSQVTQEVVDSLVGSHFEFRCRGTIKVKGKGDMVTYFLCDSGNKSLNGEVRNAMSLPQSLHAPDYYMKVSQFPENRVNTDTYSKKENGHLYAGNGVEEQQLLLQHQHKQHDPLPLPAPPPPVHHHLHQQQQQRLNSKLQKQPIFMANGGLPNIRENGNGHNGEHQQQQQQQQQHQQQQQQQQQHGGFMVATTTPPAAVAVPLQPQHHQLQFQHPHQHPLPSAVSVPVQHQILLHHQLQLQHQPVPSVMLREFNIIENPTSGGRHQQMEQLPPHHGSLDLSGMGMGVGAGVLGSDCFMMPRRDRERTYVPPLNQHGHHPPHHLHSNLNLNQSQHPPSFTSLGYGQCRESEPLLHASSVAPVAKIMPMQHAPKYEPPRYTSPHTMLSQQHQQQQQQQHQHQQPQSQSAQDQQTHPAQDPHPLQRQYAMYSQQPQLPPKPVLRTYMKPLPKLPTDLEESRDMSSTDDLSSRPHSPSMSSSDESYSKTTEGEGEGDEDSPRMVNGGHLHHRNGYHLPAGGLVNPLQWLYPCDIQVDPTSPVVDMAHLHDFELSSTTESQGHHTNSNTTSNTQHKGDSCNSFDFQKAAVGTAAGAAIATKSPFERELQRLLNESSRARCLATATTTAGAISTTDQTASNGSRELSYSLSNGKLSSANGHGVGGSGSGSGSGSGSGSAVGNGSGGSGSSNGNLSGGSGSNSNSGNNNSSHHKTEQQQNMDHEHLAGGKLLGSNSFMIAKHPVGLEAIKEITRNKNPSESSQMQTSDTESCEILHENRNQMHVLAMLEMHTAKELNGSHAHHGQHHQQPQRTHRQRPRSKELQYSHESLDGLDGAVQSQSQQRHQRYHHHHHHQQRQQQQQRYNHVQEQEERDDTEDNLADEEFEDDEVGRDVRQKRLQKSELNHKRSEVATEAGNHHDDEVEEEDDDDDEEEDHRNGGREAAPLTNGSMRGLEANVINDELKYGATHLNHQSMDSNPLESQSEWSDDDCREEATGGAESTGYITDEPGLENISLLNEAGLTDAEGALSDVNSLYNAPDVDDTSVSSRASSRLLSLDSLSGLYDCDLDSKHELAIVNASHKISSKFGQPLSPAQQQHQQQQQQQQQQQQQHHQQQLQQNPQHTQAQSHLAPVQFQSAEELRE

Target ORF information:

RefSeq Version	NM_001298317.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster rutabaga (rut), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298317.1

ORF Insert Sequence:

ATGGATCATG CCGTGAAGGC GACGCGCGGC CGCCCGTTGA ATACGCTGCG CTTCGAGAAC 
GATGAGCTGG AATGCCTCTA CCAGCGGTAT ACGCTCAAGC TGCAGCGCTT CTCGGTGCTG 
GGCGTGGTGG CTCTGGTCTT CGTCCTCTGC GGCGTGATGG CGGCCCTTTC GCTGACCTTC 
AACAACGCGG CCACCTTTCA CAACATCTTC AATGCGATCG TTTGTGGACT GTTTGCCGTG 
GTGCTGGTGC TGCTGCAGTG TTCCGTGATC AAGGACCACC ACCTGCCCAC ATTGTGCTAC 
GGAATCCTGC TATTCACGGC CAGCATCTGT GTGGTGTCGA TGCCCACATT GGGCAGCGTG 
TTCCCGGTGG ACACCAAGGA GGTGATGGCC GAGGGCGTCT GGCAGATCGT GTTCGTGGTC 
TTCCTCGCTT ACGCCATGAT GCCGCTGCAG ATTTGGGAGG CGGTGGCCTT TGGCATCGCC 
CTGCCCTCGG TGCACATAAG TCTAACGGTC TATAAGATTT TCACGGATGC GCTGCGCTAC 
CTGGAGTATA ATCAACTGAT TGCGAACATT GTCATCTTTA TTGGTGTAAA TGTGGCCGGT 
CTCGTGGTGA ACATCATGAT GGAGCGCGCA CAGCGTCGCA CATTTCTCGA TACACGCAAC 
TGCATCGCAT CCCGTCTGGA GATCCAGGAT GAGAACGAGA AACTGGAACG TCTGTTGCTA 
TCGGTGCTGC CGCAGCACGT GGCCATGCAG ATGAAGAACG ACATCCTGTC GCCGGTGGCT 
GGGCAATTTC ATCGCATCTA CATTCAGAAG CACGAGAATG TGAGCATCCT TTTCGCGGAC 
ATTGTCGGAT TCACGGTGCT CTCCTCGCAG TGTAGTGCCC AGGAGCTGGT CCGCCTGCTC 
AACGAGCTCT TTGGCCGCTT CGATCAGCTG GCACACGATA ATCACTGTTT GCGTATCAAG 
ATCCTGGGCG ACTGTTACTA TTGTGTCTCC GGATTGCCAG AACCTCGCAA GGATCACGCC 
AAATGCGCCG TGGAAATGGG ACTCGATATG ATAGATGCCA TTGCCACCGT GGTGGAGGCC 
ACCGACGTCA TCCTGAACAT GCGCGTGGGC ATCCACACGG GCCGTGTGCT CTGCGGGGTC 
TTGGGCCTTC GCAAGTGGCA GTTCGACGTG TGGTCAAACG ATGTGACCCT GGCCAATCAC 
ATGGAGTCCG GCGGGGAACC GGGTCGGGTG CACGTAACCC GCGCCACCCT GGACTCCTTG 
TCCGGCGAAT ACGAGGTGGA GGCTGGCCAC GGCGACGAAC GGTCCTCGTA TCTGCGCGAC 
CATGGTGTGG ACACGTTCTT CATCGTTCCG CCGCCACATC GACGCAAGCC ACTGATGCTC 
AACACACTGG GCGTTCGCTC GGCGATCGGC AGTCGACGGA AGTTGTCGTT CAGAAACGTG 
TCCAATGTGG TCATGCAGCT GCTGCACACG ATCAAGTTCT CGGAACCGGT GCCGTTCTCC 
AATATAGCCA CCGGATCGTT CCCCTCAGCG GCCAGCGCCT TGGGTGGCGG AGTTTCAGTC 
GGCGGTGGTG GAGGAGGAGG AGGAGGAGGC GTGGCACGGG GTAGCACCTG CGAGGCGAAC 
TCTGGAAACG TTCAGGTGTC GGAGAAGGGA TCGCGCAAGT CCCAGTCGAA GGTGGCGGAC 
AAATTCAAGA GGCCATTCCG GAAGCGGCAC TCGGTGGCCG CCCACCATCA GCCAACGAAT 
CGGGTGAATC GATTCCTTTC CCAGGCGATC AATGCCCGGT CGGTGGACTG CGACAAATCG 
GAGCATGTGG ATCGATTGAC CCTGCGATTC CGACAGAGCG ACATGGAACG CGAGTATCAC 
AAGGATTTCG ATCTGGGCTT CACTACGGCC ATGGGTTGCT CCCTGCTGTT GCTCATCCTC 
GGTGCAGCGC TCCAGGTGAC TGCTCTGCCG CGCACTCTCA TCCTGCTGCT GCTCTTTCTC 
TTTGCCTTCA TTTGGGTGAG CGCCATTCTG ATGCTCCTGC TCGCCGTGCG TCTCAAATGG 
ATCATCTGGG ATATTTCGGA GAGCTTTTCG CTGCGCATGG CCATCACGAT TTTCACGGTC 
ATACTCATCT ATTCCGTGGG ACAAGTGAAT GTGTTTACGT GCGTTTCGGA TCATCCATGC 
TCGGGCAATG GGACGACCTC CTTCCAGAAC GACTCTCATC GCAAGTGCTC GCTGCCGCAG 
TACGTGTCCC TATCGGCTGC CTTTGCCTTT CTATCTGTAT CGGTATTCCT GAGGCTGCCC 
ATCATATTCA AATCGCTCCT AGTTCTGGGC ATGGGCACCA TCTATGGCCT TTTCATAGAG 
CTATCGCATC AGAATATCTT TGAGTGCTAC GACAACCGGG TCAACGCCTC GATTCCACTT 
CACTTGATCT CGCTGGCCCG CATTGCCATC TTCATGATTG CCATTTTGGT GCATGGACGC 
CTGGTGGAGG GCACTGCCCG GCTGGACTTC CTCTGGCAGC TGCAGGCGTC GCAGGAGAAG 
AAGGAGATGG ATGTGCTACA GGAGTCCAAT AAGCGCATTC TACACAATTT GCTGCCCGCT 
CATGTGGCTG CACATTTTCT CGATGCGCAA TTTCGCAACA ACATGGAACT CTACCACCAG 
AGCTATGCCA AAGTGGGCGT CATCTTTGCA AGCGTACCGA ACTTCAACGA GTTTTACACG 
GAAATGGATG GCTCCGATCA GGGTCTTGAG TGCCTCCGTC TGCTCAACGA GATTATTGCG 
GACTTTGATG AGCTGCTCAA GGAGGATCGT TTCCGCGGCA TCGACAAGAT CAAGACCGTT 
GGTAGCACTT ATATGGCTGT CGTGGGACTC ATACCCGAGT ACAAAATCCA GCCTAACGAT 
CCGAACTCAG TGCGGCGCCA CATGACAGCG CTCATTGAGT ATGTCAAGGC CATGAGGCAC 
TCACTCCAAG AGATAAACAG TCACTCGTAC AATAACTTCA TGCTGCGCGT GGGCATCAAC 
ATTGGACCCG TTGTGGCGGG CGTAATTGGA GCTCGAAAGC CACAATATGA CATTTGGGGC 
AATACGGTGA ACGTGGCCAG TCGCATGGAC TCCACCGGAG TTCCCGGCTA CTCGCAAGTA 
ACTCAAGAGG TGGTCGACAG CCTGGTGGGG TCGCACTTTG AATTCCGCTG TCGTGGCACC 
ATTAAGGTGA AGGGCAAAGG CGACATGGTC ACATATTTCT TGTGTGACAG CGGCAACAAA 
TCGCTGAACG GAGAAGTGCG CAACGCCATG TCCCTGCCAC AGAGTCTCCA TGCGCCGGAT 
TACTATATGA AGGTGTCGCA GTTTCCGGAG AATCGCGTCA ATACCGACAC CTATAGCAAA 
AAGGAGAATG GACATCTGTA CGCTGGCAAC GGCGTCGAGG AGCAGCAGTT GTTGCTGCAG 
CATCAGCACA AACAGCACGA TCCGCTACCG CTTCCTGCTC CTCCGCCTCC GGTTCACCAT 
CATTTGCATC AGCAGCAACA GCAGCGTTTG AACAGCAAGC TGCAAAAGCA ACCGATCTTC 
ATGGCCAACG GTGGTCTGCC CAATATCCGG GAGAATGGCA ATGGTCACAA TGGAGAGCAT 
CAGCAGCAGC AGCAGCAGCA GCAGCAACAT CAGCAGCAGC AACAGCAGCA GCAGCAGCAC 
GGTGGCTTTA TGGTGGCAAC GACGACACCG CCGGCTGCAG TGGCGGTTCC CCTCCAGCCC 
CAGCACCATC AACTCCAGTT CCAGCACCCG CACCAGCATC CACTCCCGTC AGCAGTGTCG 
GTTCCAGTGC AGCACCAGAT CCTACTCCAC CACCAGCTCC AGCTCCAGCA CCAGCCCGTT 
CCTTCGGTAA TGTTGCGCGA GTTTAATATT ATTGAGAATC CGACTTCCGG TGGCCGGCAT 
CAGCAGATGG AGCAGCTGCC GCCGCATCAC GGCAGCCTCG ACCTATCGGG AATGGGAATG 
GGTGTGGGGG CTGGCGTGCT CGGCAGCGAC TGCTTCATGA TGCCACGGCG AGATCGCGAA 
CGCACATATG TGCCGCCCTT AAACCAGCAT GGCCATCATC CACCGCATCA CCTCCATTCG 
AATCTGAATC TGAATCAGAG CCAGCACCCG CCATCGTTCA CCAGCCTGGG CTATGGCCAA 
TGCCGGGAGA GCGAGCCGCT ATTGCATGCC AGCAGCGTCG CGCCGGTAGC CAAGATCATG 
CCCATGCAGC ATGCGCCAAA GTACGAGCCA CCACGTTACA CTTCTCCCCA CACCATGTTG 
TCGCAACAGC ACCAGCAGCA GCAGCAGCAG CAGCATCAGC ATCAGCAGCC GCAATCGCAA 
TCTGCCCAGG ATCAGCAGAC ACATCCCGCC CAGGACCCAC ATCCTTTGCA GCGGCAGTAC 
GCCATGTATT CGCAGCAGCC GCAGCTGCCG CCGAAGCCAG TGCTGAGAAC GTACATGAAG 
CCGTTGCCGA AGCTGCCGAC GGATCTGGAG GAGAGCCGCG ACATGTCCTC GACGGATGAT 
CTGAGCTCAC GACCACACTC GCCCTCGATG AGCAGCTCGG ATGAGAGCTA CTCGAAGACC 
ACAGAGGGCG AAGGTGAAGG CGATGAGGAC TCGCCGCGCA TGGTCAATGG TGGCCACCTG 
CATCATCGCA ATGGCTACCA CCTGCCCGCC GGTGGTCTGG TCAACCCATT GCAATGGCTC 
TACCCCTGCG ACATTCAGGT GGATCCCACC TCTCCGGTCG TCGACATGGC CCACCTGCAT 
GACTTTGAGC TAAGCTCCAC CACCGAGAGC CAGGGCCATC ACACTAACAG CAACACGACG 
AGCAACACAC AACACAAGGG CGACAGCTGC AACAGCTTCG ACTTTCAGAA GGCAGCAGTT 
GGAACGGCAG CGGGTGCGGC AATCGCGACC AAGTCGCCCT TCGAGCGTGA ACTGCAACGT 
CTGCTTAACG AATCCTCGCG AGCTCGTTGC CTGGCGACTG CGACAACGAC GGCTGGAGCC 
ATCTCTACCA CAGATCAGAC GGCCAGCAAT GGAAGCCGTG AGTTGAGCTA CTCCCTCAGC 
AATGGGAAGC TGAGCTCTGC GAATGGACAT GGCGTTGGAG GATCCGGATC CGGATCCGGA 
TCGGGATCAG GTTCAGGTTC GGCGGTGGGC AATGGCAGCG GTGGCAGCGG CAGCAGCAAT 
GGCAATCTCA GCGGCGGCAG CGGCAGCAAC TCGAACAGTG GCAACAACAA CAGCAGTCAC 
CACAAGACGG AGCAGCAGCA GAATATGGAT CACGAGCATC TGGCCGGTGG CAAATTGCTG 
GGCAGTAATA GCTTCATGAT CGCCAAACAT CCGGTGGGCT TGGAAGCCAT CAAGGAGATA 
ACGCGCAACA AGAATCCCTC GGAGTCCAGC CAAATGCAAA CCTCGGACAC GGAGTCCTGC 
GAGATTCTGC ACGAGAACCG CAACCAGATG CATGTGCTGG CCATGCTGGA GATGCATACG 
GCCAAGGAGC TGAATGGCAG CCATGCGCAT CACGGTCAGC ATCACCAGCA GCCACAGAGG 
ACGCATCGCC AGCGACCGCG CTCCAAGGAG CTGCAGTACA GCCACGAGAG TCTGGACGGA 
CTGGATGGAG CGGTGCAGTC GCAGTCGCAG CAGCGACACC AACGCTATCA TCACCACCAT 
CACCACCAAC AGAGGCAGCA GCAACAGCAG CGCTACAACC ATGTGCAGGA GCAGGAGGAG 
CGTGACGACA CCGAGGACAA CCTGGCCGAT GAGGAGTTTG AGGACGACGA AGTGGGTCGT 
GATGTGCGAC AGAAGCGCCT ACAAAAGTCC GAGCTCAATC ACAAACGATC GGAGGTTGCA 
ACAGAGGCAG GCAATCACCA CGACGACGAG GTGGAGGAGG AGGACGACGA TGACGACGAA 
GAGGAGGACC ACCGGAATGG CGGGCGGGAG GCGGCACCGC TGACTAACGG CAGCATGCGC 
GGCCTGGAGG CCAATGTGAT AAACGACGAG CTGAAGTATG GAGCAACCCA TCTCAATCAC 
CAGTCCATGG ACTCCAATCC GTTGGAGAGC CAATCGGAGT GGTCGGATGA CGATTGTCGT 
GAGGAAGCAA CAGGGGGAGC CGAGTCCACG GGCTACATTA CGGATGAGCC CGGACTGGAG 
AACATCTCGT TGCTGAACGA AGCTGGTCTC ACCGATGCCG AGGGCGCTCT CTCGGATGTC 
AACTCGCTGT ATAACGCGCC GGACGTGGAC GACACTTCGG TGTCCAGCCG GGCCAGCTCC 
CGCCTGCTTA GCCTGGACTC GCTGAGCGGC CTCTACGACT GCGATCTGGA CTCCAAGCAC 
GAGCTGGCCA TCGTCAATGC CTCGCACAAG ATCTCCAGTA AATTTGGGCA GCCTCTATCA 
CCGGCACAGC AGCAACATCA ACAACAGCAG CAACAGCAAC AGCAGCAACA GCAGCAACAC 
CACCAACAGC AGCTGCAGCA GAATCCGCAG CACACTCAAG CCCAATCGCA TTTGGCTCCG 
GTTCAGTTTC AGTCCGCCGA GGAGTTGAGA GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rut ( NM_001272620.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259549.1 Drosophila melanogaster rutabaga (rut), transcript variant D, mRNA.

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CloneID	OFa03568
Clone ID Related Accession (Same CDS sequence)	NM_001298317.1 , NM_001272620.2
Accession Version	NM_001272620.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	rutabaga, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001272620.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATCATG CCGTGAAGGC GACGCGCGGC CGCCCGTTGA ATACGCTGCG CTTCGAGAAC 
GATGAGCTGG AATGCCTCTA CCAGCGGTAT ACGCTCAAGC TGCAGCGCTT CTCGGTGCTG 
GGCGTGGTGG CTCTGGTCTT CGTCCTCTGC GGCGTGATGG CGGCCCTTTC GCTGACCTTC 
AACAACGCGG CCACCTTTCA CAACATCTTC AATGCGATCG TTTGTGGACT GTTTGCCGTG 
GTGCTGGTGC TGCTGCAGTG TTCCGTGATC AAGGACCACC ACCTGCCCAC ATTGTGCTAC 
GGAATCCTGC TATTCACGGC CAGCATCTGT GTGGTGTCGA TGCCCACATT GGGCAGCGTG 
TTCCCGGTGG ACACCAAGGA GGTGATGGCC GAGGGCGTCT GGCAGATCGT GTTCGTGGTC 
TTCCTCGCTT ACGCCATGAT GCCGCTGCAG ATTTGGGAGG CGGTGGCCTT TGGCATCGCC 
CTGCCCTCGG TGCACATAAG TCTAACGGTC TATAAGATTT TCACGGATGC GCTGCGCTAC 
CTGGAGTATA ATCAACTGAT TGCGAACATT GTCATCTTTA TTGGTGTAAA TGTGGCCGGT 
CTCGTGGTGA ACATCATGAT GGAGCGCGCA CAGCGTCGCA CATTTCTCGA TACACGCAAC 
TGCATCGCAT CCCGTCTGGA GATCCAGGAT GAGAACGAGA AACTGGAACG TCTGTTGCTA 
TCGGTGCTGC CGCAGCACGT GGCCATGCAG ATGAAGAACG ACATCCTGTC GCCGGTGGCT 
GGGCAATTTC ATCGCATCTA CATTCAGAAG CACGAGAATG TGAGCATCCT TTTCGCGGAC 
ATTGTCGGAT TCACGGTGCT CTCCTCGCAG TGTAGTGCCC AGGAGCTGGT CCGCCTGCTC 
AACGAGCTCT TTGGCCGCTT CGATCAGCTG GCACACGATA ATCACTGTTT GCGTATCAAG 
ATCCTGGGCG ACTGTTACTA TTGTGTCTCC GGATTGCCAG AACCTCGCAA GGATCACGCC 
AAATGCGCCG TGGAAATGGG ACTCGATATG ATAGATGCCA TTGCCACCGT GGTGGAGGCC 
ACCGACGTCA TCCTGAACAT GCGCGTGGGC ATCCACACGG GCCGTGTGCT CTGCGGGGTC 
TTGGGCCTTC GCAAGTGGCA GTTCGACGTG TGGTCAAACG ATGTGACCCT GGCCAATCAC 
ATGGAGTCCG GCGGGGAACC GGGTCGGGTG CACGTAACCC GCGCCACCCT GGACTCCTTG 
TCCGGCGAAT ACGAGGTGGA GGCTGGCCAC GGCGACGAAC GGTCCTCGTA TCTGCGCGAC 
CATGGTGTGG ACACGTTCTT CATCGTTCCG CCGCCACATC GACGCAAGCC ACTGATGCTC 
AACACACTGG GCGTTCGCTC GGCGATCGGC AGTCGACGGA AGTTGTCGTT CAGAAACGTG 
TCCAATGTGG TCATGCAGCT GCTGCACACG ATCAAGTTCT CGGAACCGGT GCCGTTCTCC 
AATATAGCCA CCGGATCGTT CCCCTCAGCG GCCAGCGCCT TGGGTGGCGG AGTTTCAGTC 
GGCGGTGGTG GAGGAGGAGG AGGAGGAGGC GTGGCACGGG GTAGCACCTG CGAGGCGAAC 
TCTGGAAACG TTCAGGTGTC GGAGAAGGGA TCGCGCAAGT CCCAGTCGAA GGTGGCGGAC 
AAATTCAAGA GGCCATTCCG GAAGCGGCAC TCGGTGGCCG CCCACCATCA GCCAACGAAT 
CGGGTGAATC GATTCCTTTC CCAGGCGATC AATGCCCGGT CGGTGGACTG CGACAAATCG 
GAGCATGTGG ATCGATTGAC CCTGCGATTC CGACAGAGCG ACATGGAACG CGAGTATCAC 
AAGGATTTCG ATCTGGGCTT CACTACGGCC ATGGGTTGCT CCCTGCTGTT GCTCATCCTC 
GGTGCAGCGC TCCAGGTGAC TGCTCTGCCG CGCACTCTCA TCCTGCTGCT GCTCTTTCTC 
TTTGCCTTCA TTTGGGTGAG CGCCATTCTG ATGCTCCTGC TCGCCGTGCG TCTCAAATGG 
ATCATCTGGG ATATTTCGGA GAGCTTTTCG CTGCGCATGG CCATCACGAT TTTCACGGTC 
ATACTCATCT ATTCCGTGGG ACAAGTGAAT GTGTTTACGT GCGTTTCGGA TCATCCATGC 
TCGGGCAATG GGACGACCTC CTTCCAGAAC GACTCTCATC GCAAGTGCTC GCTGCCGCAG 
TACGTGTCCC TATCGGCTGC CTTTGCCTTT CTATCTGTAT CGGTATTCCT GAGGCTGCCC 
ATCATATTCA AATCGCTCCT AGTTCTGGGC ATGGGCACCA TCTATGGCCT TTTCATAGAG 
CTATCGCATC AGAATATCTT TGAGTGCTAC GACAACCGGG TCAACGCCTC GATTCCACTT 
CACTTGATCT CGCTGGCCCG CATTGCCATC TTCATGATTG CCATTTTGGT GCATGGACGC 
CTGGTGGAGG GCACTGCCCG GCTGGACTTC CTCTGGCAGC TGCAGGCGTC GCAGGAGAAG 
AAGGAGATGG ATGTGCTACA GGAGTCCAAT AAGCGCATTC TACACAATTT GCTGCCCGCT 
CATGTGGCTG CACATTTTCT CGATGCGCAA TTTCGCAACA ACATGGAACT CTACCACCAG 
AGCTATGCCA AAGTGGGCGT CATCTTTGCA AGCGTACCGA ACTTCAACGA GTTTTACACG 
GAAATGGATG GCTCCGATCA GGGTCTTGAG TGCCTCCGTC TGCTCAACGA GATTATTGCG 
GACTTTGATG AGCTGCTCAA GGAGGATCGT TTCCGCGGCA TCGACAAGAT CAAGACCGTT 
GGTAGCACTT ATATGGCTGT CGTGGGACTC ATACCCGAGT ACAAAATCCA GCCTAACGAT 
CCGAACTCAG TGCGGCGCCA CATGACAGCG CTCATTGAGT ATGTCAAGGC CATGAGGCAC 
TCACTCCAAG AGATAAACAG TCACTCGTAC AATAACTTCA TGCTGCGCGT GGGCATCAAC 
ATTGGACCCG TTGTGGCGGG CGTAATTGGA GCTCGAAAGC CACAATATGA CATTTGGGGC 
AATACGGTGA ACGTGGCCAG TCGCATGGAC TCCACCGGAG TTCCCGGCTA CTCGCAAGTA 
ACTCAAGAGG TGGTCGACAG CCTGGTGGGG TCGCACTTTG AATTCCGCTG TCGTGGCACC 
ATTAAGGTGA AGGGCAAAGG CGACATGGTC ACATATTTCT TGTGTGACAG CGGCAACAAA 
TCGCTGAACG GAGAAGTGCG CAACGCCATG TCCCTGCCAC AGAGTCTCCA TGCGCCGGAT 
TACTATATGA AGGTGTCGCA GTTTCCGGAG AATCGCGTCA ATACCGACAC CTATAGCAAA 
AAGGAGAATG GACATCTGTA CGCTGGCAAC GGCGTCGAGG AGCAGCAGTT GTTGCTGCAG 
CATCAGCACA AACAGCACGA TCCGCTACCG CTTCCTGCTC CTCCGCCTCC GGTTCACCAT 
CATTTGCATC AGCAGCAACA GCAGCGTTTG AACAGCAAGC TGCAAAAGCA ACCGATCTTC 
ATGGCCAACG GTGGTCTGCC CAATATCCGG GAGAATGGCA ATGGTCACAA TGGAGAGCAT 
CAGCAGCAGC AGCAGCAGCA GCAGCAACAT CAGCAGCAGC AACAGCAGCA GCAGCAGCAC 
GGTGGCTTTA TGGTGGCAAC GACGACACCG CCGGCTGCAG TGGCGGTTCC CCTCCAGCCC 
CAGCACCATC AACTCCAGTT CCAGCACCCG CACCAGCATC CACTCCCGTC AGCAGTGTCG 
GTTCCAGTGC AGCACCAGAT CCTACTCCAC CACCAGCTCC AGCTCCAGCA CCAGCCCGTT 
CCTTCGGTAA TGTTGCGCGA GTTTAATATT ATTGAGAATC CGACTTCCGG TGGCCGGCAT 
CAGCAGATGG AGCAGCTGCC GCCGCATCAC GGCAGCCTCG ACCTATCGGG AATGGGAATG 
GGTGTGGGGG CTGGCGTGCT CGGCAGCGAC TGCTTCATGA TGCCACGGCG AGATCGCGAA 
CGCACATATG TGCCGCCCTT AAACCAGCAT GGCCATCATC CACCGCATCA CCTCCATTCG 
AATCTGAATC TGAATCAGAG CCAGCACCCG CCATCGTTCA CCAGCCTGGG CTATGGCCAA 
TGCCGGGAGA GCGAGCCGCT ATTGCATGCC AGCAGCGTCG CGCCGGTAGC CAAGATCATG 
CCCATGCAGC ATGCGCCAAA GTACGAGCCA CCACGTTACA CTTCTCCCCA CACCATGTTG 
TCGCAACAGC ACCAGCAGCA GCAGCAGCAG CAGCATCAGC ATCAGCAGCC GCAATCGCAA 
TCTGCCCAGG ATCAGCAGAC ACATCCCGCC CAGGACCCAC ATCCTTTGCA GCGGCAGTAC 
GCCATGTATT CGCAGCAGCC GCAGCTGCCG CCGAAGCCAG TGCTGAGAAC GTACATGAAG 
CCGTTGCCGA AGCTGCCGAC GGATCTGGAG GAGAGCCGCG ACATGTCCTC GACGGATGAT 
CTGAGCTCAC GACCACACTC GCCCTCGATG AGCAGCTCGG ATGAGAGCTA CTCGAAGACC 
ACAGAGGGCG AAGGTGAAGG CGATGAGGAC TCGCCGCGCA TGGTCAATGG TGGCCACCTG 
CATCATCGCA ATGGCTACCA CCTGCCCGCC GGTGGTCTGG TCAACCCATT GCAATGGCTC 
TACCCCTGCG ACATTCAGGT GGATCCCACC TCTCCGGTCG TCGACATGGC CCACCTGCAT 
GACTTTGAGC TAAGCTCCAC CACCGAGAGC CAGGGCCATC ACACTAACAG CAACACGACG 
AGCAACACAC AACACAAGGG CGACAGCTGC AACAGCTTCG ACTTTCAGAA GGCAGCAGTT 
GGAACGGCAG CGGGTGCGGC AATCGCGACC AAGTCGCCCT TCGAGCGTGA ACTGCAACGT 
CTGCTTAACG AATCCTCGCG AGCTCGTTGC CTGGCGACTG CGACAACGAC GGCTGGAGCC 
ATCTCTACCA CAGATCAGAC GGCCAGCAAT GGAAGCCGTG AGTTGAGCTA CTCCCTCAGC 
AATGGGAAGC TGAGCTCTGC GAATGGACAT GGCGTTGGAG GATCCGGATC CGGATCCGGA 
TCGGGATCAG GTTCAGGTTC GGCGGTGGGC AATGGCAGCG GTGGCAGCGG CAGCAGCAAT 
GGCAATCTCA GCGGCGGCAG CGGCAGCAAC TCGAACAGTG GCAACAACAA CAGCAGTCAC 
CACAAGACGG AGCAGCAGCA GAATATGGAT CACGAGCATC TGGCCGGTGG CAAATTGCTG 
GGCAGTAATA GCTTCATGAT CGCCAAACAT CCGGTGGGCT TGGAAGCCAT CAAGGAGATA 
ACGCGCAACA AGAATCCCTC GGAGTCCAGC CAAATGCAAA CCTCGGACAC GGAGTCCTGC 
GAGATTCTGC ACGAGAACCG CAACCAGATG CATGTGCTGG CCATGCTGGA GATGCATACG 
GCCAAGGAGC TGAATGGCAG CCATGCGCAT CACGGTCAGC ATCACCAGCA GCCACAGAGG 
ACGCATCGCC AGCGACCGCG CTCCAAGGAG CTGCAGTACA GCCACGAGAG TCTGGACGGA 
CTGGATGGAG CGGTGCAGTC GCAGTCGCAG CAGCGACACC AACGCTATCA TCACCACCAT 
CACCACCAAC AGAGGCAGCA GCAACAGCAG CGCTACAACC ATGTGCAGGA GCAGGAGGAG 
CGTGACGACA CCGAGGACAA CCTGGCCGAT GAGGAGTTTG AGGACGACGA AGTGGGTCGT 
GATGTGCGAC AGAAGCGCCT ACAAAAGTCC GAGCTCAATC ACAAACGATC GGAGGTTGCA 
ACAGAGGCAG GCAATCACCA CGACGACGAG GTGGAGGAGG AGGACGACGA TGACGACGAA 
GAGGAGGACC ACCGGAATGG CGGGCGGGAG GCGGCACCGC TGACTAACGG CAGCATGCGC 
GGCCTGGAGG CCAATGTGAT AAACGACGAG CTGAAGTATG GAGCAACCCA TCTCAATCAC 
CAGTCCATGG ACTCCAATCC GTTGGAGAGC CAATCGGAGT GGTCGGATGA CGATTGTCGT 
GAGGAAGCAA CAGGGGGAGC CGAGTCCACG GGCTACATTA CGGATGAGCC CGGACTGGAG 
AACATCTCGT TGCTGAACGA AGCTGGTCTC ACCGATGCCG AGGGCGCTCT CTCGGATGTC 
AACTCGCTGT ATAACGCGCC GGACGTGGAC GACACTTCGG TGTCCAGCCG GGCCAGCTCC 
CGCCTGCTTA GCCTGGACTC GCTGAGCGGC CTCTACGACT GCGATCTGGA CTCCAAGCAC 
GAGCTGGCCA TCGTCAATGC CTCGCACAAG ATCTCCAGTA AATTTGGGCA GCCTCTATCA 
CCGGCACAGC AGCAACATCA ACAACAGCAG CAACAGCAAC AGCAGCAACA GCAGCAACAC 
CACCAACAGC AGCTGCAGCA GAATCCGCAG CACACTCAAG CCCAATCGCA TTTGGCTCCG 
GTTCAGTTTC AGTCCGCCGA GGAGTTGAGA GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259549.1
CDS	269..6784
Translation	MDHAVKATRGRPLNTLRFENDELECLYQRYTLKLQRFSVLGVVALVFVLCGVMAALSLTFNNAATFHNIFNAIVCGLFAVVLVLLQCSVIKDHHLPTLCYGILLFTASICVVSMPTLGSVFPVDTKEVMAEGVWQIVFVVFLAYAMMPLQIWEAVAFGIALPSVHISLTVYKIFTDALRYLEYNQLIANIVIFIGVNVAGLVVNIMMERAQRRTFLDTRNCIASRLEIQDENEKLERLLLSVLPQHVAMQMKNDILSPVAGQFHRIYIQKHENVSILFADIVGFTVLSSQCSAQELVRLLNELFGRFDQLAHDNHCLRIKILGDCYYCVSGLPEPRKDHAKCAVEMGLDMIDAIATVVEATDVILNMRVGIHTGRVLCGVLGLRKWQFDVWSNDVTLANHMESGGEPGRVHVTRATLDSLSGEYEVEAGHGDERSSYLRDHGVDTFFIVPPPHRRKPLMLNTLGVRSAIGSRRKLSFRNVSNVVMQLLHTIKFSEPVPFSNIATGSFPSAASALGGGVSVGGGGGGGGGGVARGSTCEANSGNVQVSEKGSRKSQSKVADKFKRPFRKRHSVAAHHQPTNRVNRFLSQAINARSVDCDKSEHVDRLTLRFRQSDMEREYHKDFDLGFTTAMGCSLLLLILGAALQVTALPRTLILLLLFLFAFIWVSAILMLLLAVRLKWIIWDISESFSLRMAITIFTVILIYSVGQVNVFTCVSDHPCSGNGTTSFQNDSHRKCSLPQYVSLSAAFAFLSVSVFLRLPIIFKSLLVLGMGTIYGLFIELSHQNIFECYDNRVNASIPLHLISLARIAIFMIAILVHGRLVEGTARLDFLWQLQASQEKKEMDVLQESNKRILHNLLPAHVAAHFLDAQFRNNMELYHQSYAKVGVIFASVPNFNEFYTEMDGSDQGLECLRLLNEIIADFDELLKEDRFRGIDKIKTVGSTYMAVVGLIPEYKIQPNDPNSVRRHMTALIEYVKAMRHSLQEINSHSYNNFMLRVGINIGPVVAGVIGARKPQYDIWGNTVNVASRMDSTGVPGYSQVTQEVVDSLVGSHFEFRCRGTIKVKGKGDMVTYFLCDSGNKSLNGEVRNAMSLPQSLHAPDYYMKVSQFPENRVNTDTYSKKENGHLYAGNGVEEQQLLLQHQHKQHDPLPLPAPPPPVHHHLHQQQQQRLNSKLQKQPIFMANGGLPNIRENGNGHNGEHQQQQQQQQQHQQQQQQQQQHGGFMVATTTPPAAVAVPLQPQHHQLQFQHPHQHPLPSAVSVPVQHQILLHHQLQLQHQPVPSVMLREFNIIENPTSGGRHQQMEQLPPHHGSLDLSGMGMGVGAGVLGSDCFMMPRRDRERTYVPPLNQHGHHPPHHLHSNLNLNQSQHPPSFTSLGYGQCRESEPLLHASSVAPVAKIMPMQHAPKYEPPRYTSPHTMLSQQHQQQQQQQHQHQQPQSQSAQDQQTHPAQDPHPLQRQYAMYSQQPQLPPKPVLRTYMKPLPKLPTDLEESRDMSSTDDLSSRPHSPSMSSSDESYSKTTEGEGEGDEDSPRMVNGGHLHHRNGYHLPAGGLVNPLQWLYPCDIQVDPTSPVVDMAHLHDFELSSTTESQGHHTNSNTTSNTQHKGDSCNSFDFQKAAVGTAAGAAIATKSPFERELQRLLNESSRARCLATATTTAGAISTTDQTASNGSRELSYSLSNGKLSSANGHGVGGSGSGSGSGSGSGSAVGNGSGGSGSSNGNLSGGSGSNSNSGNNNSSHHKTEQQQNMDHEHLAGGKLLGSNSFMIAKHPVGLEAIKEITRNKNPSESSQMQTSDTESCEILHENRNQMHVLAMLEMHTAKELNGSHAHHGQHHQQPQRTHRQRPRSKELQYSHESLDGLDGAVQSQSQQRHQRYHHHHHHQQRQQQQQRYNHVQEQEERDDTEDNLADEEFEDDEVGRDVRQKRLQKSELNHKRSEVATEAGNHHDDEVEEEDDDDDEEEDHRNGGREAAPLTNGSMRGLEANVINDELKYGATHLNHQSMDSNPLESQSEWSDDDCREEATGGAESTGYITDEPGLENISLLNEAGLTDAEGALSDVNSLYNAPDVDDTSVSSRASSRLLSLDSLSGLYDCDLDSKHELAIVNASHKISSKFGQPLSPAQQQHQQQQQQQQQQQQQHHQQQLQQNPQHTQAQSHLAPVQFQSAEELRE

Target ORF information:

RefSeq Version	NM_001272620.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster rutabaga (rut), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272620.2

ORF Insert Sequence:

ATGGATCATG CCGTGAAGGC GACGCGCGGC CGCCCGTTGA ATACGCTGCG CTTCGAGAAC 
GATGAGCTGG AATGCCTCTA CCAGCGGTAT ACGCTCAAGC TGCAGCGCTT CTCGGTGCTG 
GGCGTGGTGG CTCTGGTCTT CGTCCTCTGC GGCGTGATGG CGGCCCTTTC GCTGACCTTC 
AACAACGCGG CCACCTTTCA CAACATCTTC AATGCGATCG TTTGTGGACT GTTTGCCGTG 
GTGCTGGTGC TGCTGCAGTG TTCCGTGATC AAGGACCACC ACCTGCCCAC ATTGTGCTAC 
GGAATCCTGC TATTCACGGC CAGCATCTGT GTGGTGTCGA TGCCCACATT GGGCAGCGTG 
TTCCCGGTGG ACACCAAGGA GGTGATGGCC GAGGGCGTCT GGCAGATCGT GTTCGTGGTC 
TTCCTCGCTT ACGCCATGAT GCCGCTGCAG ATTTGGGAGG CGGTGGCCTT TGGCATCGCC 
CTGCCCTCGG TGCACATAAG TCTAACGGTC TATAAGATTT TCACGGATGC GCTGCGCTAC 
CTGGAGTATA ATCAACTGAT TGCGAACATT GTCATCTTTA TTGGTGTAAA TGTGGCCGGT 
CTCGTGGTGA ACATCATGAT GGAGCGCGCA CAGCGTCGCA CATTTCTCGA TACACGCAAC 
TGCATCGCAT CCCGTCTGGA GATCCAGGAT GAGAACGAGA AACTGGAACG TCTGTTGCTA 
TCGGTGCTGC CGCAGCACGT GGCCATGCAG ATGAAGAACG ACATCCTGTC GCCGGTGGCT 
GGGCAATTTC ATCGCATCTA CATTCAGAAG CACGAGAATG TGAGCATCCT TTTCGCGGAC 
ATTGTCGGAT TCACGGTGCT CTCCTCGCAG TGTAGTGCCC AGGAGCTGGT CCGCCTGCTC 
AACGAGCTCT TTGGCCGCTT CGATCAGCTG GCACACGATA ATCACTGTTT GCGTATCAAG 
ATCCTGGGCG ACTGTTACTA TTGTGTCTCC GGATTGCCAG AACCTCGCAA GGATCACGCC 
AAATGCGCCG TGGAAATGGG ACTCGATATG ATAGATGCCA TTGCCACCGT GGTGGAGGCC 
ACCGACGTCA TCCTGAACAT GCGCGTGGGC ATCCACACGG GCCGTGTGCT CTGCGGGGTC 
TTGGGCCTTC GCAAGTGGCA GTTCGACGTG TGGTCAAACG ATGTGACCCT GGCCAATCAC 
ATGGAGTCCG GCGGGGAACC GGGTCGGGTG CACGTAACCC GCGCCACCCT GGACTCCTTG 
TCCGGCGAAT ACGAGGTGGA GGCTGGCCAC GGCGACGAAC GGTCCTCGTA TCTGCGCGAC 
CATGGTGTGG ACACGTTCTT CATCGTTCCG CCGCCACATC GACGCAAGCC ACTGATGCTC 
AACACACTGG GCGTTCGCTC GGCGATCGGC AGTCGACGGA AGTTGTCGTT CAGAAACGTG 
TCCAATGTGG TCATGCAGCT GCTGCACACG ATCAAGTTCT CGGAACCGGT GCCGTTCTCC 
AATATAGCCA CCGGATCGTT CCCCTCAGCG GCCAGCGCCT TGGGTGGCGG AGTTTCAGTC 
GGCGGTGGTG GAGGAGGAGG AGGAGGAGGC GTGGCACGGG GTAGCACCTG CGAGGCGAAC 
TCTGGAAACG TTCAGGTGTC GGAGAAGGGA TCGCGCAAGT CCCAGTCGAA GGTGGCGGAC 
AAATTCAAGA GGCCATTCCG GAAGCGGCAC TCGGTGGCCG CCCACCATCA GCCAACGAAT 
CGGGTGAATC GATTCCTTTC CCAGGCGATC AATGCCCGGT CGGTGGACTG CGACAAATCG 
GAGCATGTGG ATCGATTGAC CCTGCGATTC CGACAGAGCG ACATGGAACG CGAGTATCAC 
AAGGATTTCG ATCTGGGCTT CACTACGGCC ATGGGTTGCT CCCTGCTGTT GCTCATCCTC 
GGTGCAGCGC TCCAGGTGAC TGCTCTGCCG CGCACTCTCA TCCTGCTGCT GCTCTTTCTC 
TTTGCCTTCA TTTGGGTGAG CGCCATTCTG ATGCTCCTGC TCGCCGTGCG TCTCAAATGG 
ATCATCTGGG ATATTTCGGA GAGCTTTTCG CTGCGCATGG CCATCACGAT TTTCACGGTC 
ATACTCATCT ATTCCGTGGG ACAAGTGAAT GTGTTTACGT GCGTTTCGGA TCATCCATGC 
TCGGGCAATG GGACGACCTC CTTCCAGAAC GACTCTCATC GCAAGTGCTC GCTGCCGCAG 
TACGTGTCCC TATCGGCTGC CTTTGCCTTT CTATCTGTAT CGGTATTCCT GAGGCTGCCC 
ATCATATTCA AATCGCTCCT AGTTCTGGGC ATGGGCACCA TCTATGGCCT TTTCATAGAG 
CTATCGCATC AGAATATCTT TGAGTGCTAC GACAACCGGG TCAACGCCTC GATTCCACTT 
CACTTGATCT CGCTGGCCCG CATTGCCATC TTCATGATTG CCATTTTGGT GCATGGACGC 
CTGGTGGAGG GCACTGCCCG GCTGGACTTC CTCTGGCAGC TGCAGGCGTC GCAGGAGAAG 
AAGGAGATGG ATGTGCTACA GGAGTCCAAT AAGCGCATTC TACACAATTT GCTGCCCGCT 
CATGTGGCTG CACATTTTCT CGATGCGCAA TTTCGCAACA ACATGGAACT CTACCACCAG 
AGCTATGCCA AAGTGGGCGT CATCTTTGCA AGCGTACCGA ACTTCAACGA GTTTTACACG 
GAAATGGATG GCTCCGATCA GGGTCTTGAG TGCCTCCGTC TGCTCAACGA GATTATTGCG 
GACTTTGATG AGCTGCTCAA GGAGGATCGT TTCCGCGGCA TCGACAAGAT CAAGACCGTT 
GGTAGCACTT ATATGGCTGT CGTGGGACTC ATACCCGAGT ACAAAATCCA GCCTAACGAT 
CCGAACTCAG TGCGGCGCCA CATGACAGCG CTCATTGAGT ATGTCAAGGC CATGAGGCAC 
TCACTCCAAG AGATAAACAG TCACTCGTAC AATAACTTCA TGCTGCGCGT GGGCATCAAC 
ATTGGACCCG TTGTGGCGGG CGTAATTGGA GCTCGAAAGC CACAATATGA CATTTGGGGC 
AATACGGTGA ACGTGGCCAG TCGCATGGAC TCCACCGGAG TTCCCGGCTA CTCGCAAGTA 
ACTCAAGAGG TGGTCGACAG CCTGGTGGGG TCGCACTTTG AATTCCGCTG TCGTGGCACC 
ATTAAGGTGA AGGGCAAAGG CGACATGGTC ACATATTTCT TGTGTGACAG CGGCAACAAA 
TCGCTGAACG GAGAAGTGCG CAACGCCATG TCCCTGCCAC AGAGTCTCCA TGCGCCGGAT 
TACTATATGA AGGTGTCGCA GTTTCCGGAG AATCGCGTCA ATACCGACAC CTATAGCAAA 
AAGGAGAATG GACATCTGTA CGCTGGCAAC GGCGTCGAGG AGCAGCAGTT GTTGCTGCAG 
CATCAGCACA AACAGCACGA TCCGCTACCG CTTCCTGCTC CTCCGCCTCC GGTTCACCAT 
CATTTGCATC AGCAGCAACA GCAGCGTTTG AACAGCAAGC TGCAAAAGCA ACCGATCTTC 
ATGGCCAACG GTGGTCTGCC CAATATCCGG GAGAATGGCA ATGGTCACAA TGGAGAGCAT 
CAGCAGCAGC AGCAGCAGCA GCAGCAACAT CAGCAGCAGC AACAGCAGCA GCAGCAGCAC 
GGTGGCTTTA TGGTGGCAAC GACGACACCG CCGGCTGCAG TGGCGGTTCC CCTCCAGCCC 
CAGCACCATC AACTCCAGTT CCAGCACCCG CACCAGCATC CACTCCCGTC AGCAGTGTCG 
GTTCCAGTGC AGCACCAGAT CCTACTCCAC CACCAGCTCC AGCTCCAGCA CCAGCCCGTT 
CCTTCGGTAA TGTTGCGCGA GTTTAATATT ATTGAGAATC CGACTTCCGG TGGCCGGCAT 
CAGCAGATGG AGCAGCTGCC GCCGCATCAC GGCAGCCTCG ACCTATCGGG AATGGGAATG 
GGTGTGGGGG CTGGCGTGCT CGGCAGCGAC TGCTTCATGA TGCCACGGCG AGATCGCGAA 
CGCACATATG TGCCGCCCTT AAACCAGCAT GGCCATCATC CACCGCATCA CCTCCATTCG 
AATCTGAATC TGAATCAGAG CCAGCACCCG CCATCGTTCA CCAGCCTGGG CTATGGCCAA 
TGCCGGGAGA GCGAGCCGCT ATTGCATGCC AGCAGCGTCG CGCCGGTAGC CAAGATCATG 
CCCATGCAGC ATGCGCCAAA GTACGAGCCA CCACGTTACA CTTCTCCCCA CACCATGTTG 
TCGCAACAGC ACCAGCAGCA GCAGCAGCAG CAGCATCAGC ATCAGCAGCC GCAATCGCAA 
TCTGCCCAGG ATCAGCAGAC ACATCCCGCC CAGGACCCAC ATCCTTTGCA GCGGCAGTAC 
GCCATGTATT CGCAGCAGCC GCAGCTGCCG CCGAAGCCAG TGCTGAGAAC GTACATGAAG 
CCGTTGCCGA AGCTGCCGAC GGATCTGGAG GAGAGCCGCG ACATGTCCTC GACGGATGAT 
CTGAGCTCAC GACCACACTC GCCCTCGATG AGCAGCTCGG ATGAGAGCTA CTCGAAGACC 
ACAGAGGGCG AAGGTGAAGG CGATGAGGAC TCGCCGCGCA TGGTCAATGG TGGCCACCTG 
CATCATCGCA ATGGCTACCA CCTGCCCGCC GGTGGTCTGG TCAACCCATT GCAATGGCTC 
TACCCCTGCG ACATTCAGGT GGATCCCACC TCTCCGGTCG TCGACATGGC CCACCTGCAT 
GACTTTGAGC TAAGCTCCAC CACCGAGAGC CAGGGCCATC ACACTAACAG CAACACGACG 
AGCAACACAC AACACAAGGG CGACAGCTGC AACAGCTTCG ACTTTCAGAA GGCAGCAGTT 
GGAACGGCAG CGGGTGCGGC AATCGCGACC AAGTCGCCCT TCGAGCGTGA ACTGCAACGT 
CTGCTTAACG AATCCTCGCG AGCTCGTTGC CTGGCGACTG CGACAACGAC GGCTGGAGCC 
ATCTCTACCA CAGATCAGAC GGCCAGCAAT GGAAGCCGTG AGTTGAGCTA CTCCCTCAGC 
AATGGGAAGC TGAGCTCTGC GAATGGACAT GGCGTTGGAG GATCCGGATC CGGATCCGGA 
TCGGGATCAG GTTCAGGTTC GGCGGTGGGC AATGGCAGCG GTGGCAGCGG CAGCAGCAAT 
GGCAATCTCA GCGGCGGCAG CGGCAGCAAC TCGAACAGTG GCAACAACAA CAGCAGTCAC 
CACAAGACGG AGCAGCAGCA GAATATGGAT CACGAGCATC TGGCCGGTGG CAAATTGCTG 
GGCAGTAATA GCTTCATGAT CGCCAAACAT CCGGTGGGCT TGGAAGCCAT CAAGGAGATA 
ACGCGCAACA AGAATCCCTC GGAGTCCAGC CAAATGCAAA CCTCGGACAC GGAGTCCTGC 
GAGATTCTGC ACGAGAACCG CAACCAGATG CATGTGCTGG CCATGCTGGA GATGCATACG 
GCCAAGGAGC TGAATGGCAG CCATGCGCAT CACGGTCAGC ATCACCAGCA GCCACAGAGG 
ACGCATCGCC AGCGACCGCG CTCCAAGGAG CTGCAGTACA GCCACGAGAG TCTGGACGGA 
CTGGATGGAG CGGTGCAGTC GCAGTCGCAG CAGCGACACC AACGCTATCA TCACCACCAT 
CACCACCAAC AGAGGCAGCA GCAACAGCAG CGCTACAACC ATGTGCAGGA GCAGGAGGAG 
CGTGACGACA CCGAGGACAA CCTGGCCGAT GAGGAGTTTG AGGACGACGA AGTGGGTCGT 
GATGTGCGAC AGAAGCGCCT ACAAAAGTCC GAGCTCAATC ACAAACGATC GGAGGTTGCA 
ACAGAGGCAG GCAATCACCA CGACGACGAG GTGGAGGAGG AGGACGACGA TGACGACGAA 
GAGGAGGACC ACCGGAATGG CGGGCGGGAG GCGGCACCGC TGACTAACGG CAGCATGCGC 
GGCCTGGAGG CCAATGTGAT AAACGACGAG CTGAAGTATG GAGCAACCCA TCTCAATCAC 
CAGTCCATGG ACTCCAATCC GTTGGAGAGC CAATCGGAGT GGTCGGATGA CGATTGTCGT 
GAGGAAGCAA CAGGGGGAGC CGAGTCCACG GGCTACATTA CGGATGAGCC CGGACTGGAG 
AACATCTCGT TGCTGAACGA AGCTGGTCTC ACCGATGCCG AGGGCGCTCT CTCGGATGTC 
AACTCGCTGT ATAACGCGCC GGACGTGGAC GACACTTCGG TGTCCAGCCG GGCCAGCTCC 
CGCCTGCTTA GCCTGGACTC GCTGAGCGGC CTCTACGACT GCGATCTGGA CTCCAAGCAC 
GAGCTGGCCA TCGTCAATGC CTCGCACAAG ATCTCCAGTA AATTTGGGCA GCCTCTATCA 
CCGGCACAGC AGCAACATCA ACAACAGCAG CAACAGCAAC AGCAGCAACA GCAGCAACAC 
CACCAACAGC AGCTGCAGCA GAATCCGCAG CACACTCAAG CCCAATCGCA TTTGGCTCCG 
GTTCAGTTTC AGTCCGCCGA GGAGTTGAGA GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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rut cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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