NetB cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	NetB
Entrez Gene ID	32400
Full Name	Netrin-B
Synonyms	CG10521,CT29512,Dmel\CG10521,NETB,net,netB,netrin,netrin B
General protein information	Preferred Names Netrin-B Names CG10521 gene product from transcript CG10521-RD CG10521-PA CG10521-PB CG10521-PC CG10521-PD CG10521-PE CG10521-PF CG10521-PG D-netrin D-netrin-B NetB-PA NetB-PB NetB-PC NetB-PD NetB-PE NetB-PF NetB-PG netrin netrin B netrin-B netrinB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 12F2-12F3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001169284.2	NP_001162755.1	Netrin-B, isoform D
NM_001258740.2	NP_001245669.1	Netrin-B, isoform F
NM_078600.4	NP_511155.1	Netrin-B, isoform A
NM_001169282.1	NP_001162753.1	Netrin-B, isoform B
NM_001169283.2	NP_001162754.1	Netrin-B, isoform C
NM_001258739.2	NP_001245668.1	Netrin-B, isoform E
NM_001272617.1	NP_001259546.1	Netrin-B, isoform G

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ntn1	NP_446183.2
Homo sapiens (human)	NTN1	NP_004813.2
Canis lupus familiaris (dog)	NTN1	XP_850304.2
Mus musculus (house mouse)	Ntn1	NP_032770.2
Gallus gallus (chicken)	NTN1	NP_990750.1
Bos taurus (cattle)	NTN1	NP_001179495.1
Danio rerio (zebrafish)	ntn1a	NP_571104.1
Drosophila melanogaster (fruit fly)	NetB	NP_511155.1
Caenorhabditis elegans (roundworm)	unc-6	NP_509165.1
	NTN1	XP_001113322.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000228	XP_310904.5
Pan troglodytes (chimpanzee)	NTN1	XP_511846.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

mutation of mushroom body defect (mud) dramatically enhances the phenotype of Netrin or frazzled mutants, resulting in many more axons failing to cross the midline, although mutations in mud alone have little effect.
Title: Mushroom body defect is required in parallel to Netrin for midline axon guidance in Drosophila.

Contact-mediated cell adhesion via Fmi acts in addition to the Net/Fra guidance system during axon pathfinding across the midline, underlining the importance of PCP molecules during vertebrates and invertebrates midline development.
Title: Flamingo, a seven-pass transmembrane cadherin, cooperates with Netrin/Frazzled in Drosophila midline guidance.

These data are the first to show that Netrin and Frazzled regulate the placement of gap junctions presynaptically at a synapse.
Title: Netrin and Frazzled regulate presynaptic gap junctions at a Drosophila giant synapse.

Data find that Abl interacts both physically and genetically with the Netrin receptor Frazzled, and that disrupting this interaction prevents Abl from promoting midline axon crossing.
Title: Distinct functional domains of the Abelson tyrosine kinase control axon guidance responses to Netrin and Slit to regulate the assembly of neural circuits.

adult Drosophila lacking both netrin genes, NetA and NetB, have fertility defects in both sexes together with an inability to fly and reduced viability.
Title: Control of male and female fertility by the netrin axon guidance genes.

The following NetB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NetB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01603	NM_001169283.2 Latest version!	Drosophila melanogaster Netrin-B (NetB), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa01603	NM_001258739.2 Latest version!	Drosophila melanogaster Netrin-B (NetB), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa01603	NM_078600.4 Latest version!	Drosophila melanogaster Netrin-B (NetB), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa01603	NM_001169282.1 Latest version!	Drosophila melanogaster Netrin-B (NetB), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa01603	NM_001169284.2 Latest version!	Drosophila melanogaster Netrin-B (NetB), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa01603	NM_001258740.2 Latest version!	Drosophila melanogaster Netrin-B (NetB), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa03563	NM_001272617.1 Latest version!	Drosophila melanogaster Netrin-B (NetB), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NetB ( NM_001169284.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001162755.1 Drosophila melanogaster Netrin-B (NetB), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa01603
Clone ID Related Accession (Same CDS sequence)	NM_001169284.2 , NM_001258740.2 , NM_078600.4 , NM_001169282.1 , NM_001169283.2 , NM_001258739.2
Accession Version	NM_001169284.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2382bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Netrin-B, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_001169284.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTCAGAG CGACAGGGAC AAGGATGGGC CTGCTCCTGC CCATAATCCT GGCACTGGCC 
ATCGGATCCT CGGCCGCCGG CATATCCTCC AACGATCCCT GCTACTTCGA GGGCAAGCCG 
CGCAAGTGCC TGCCAAGCTT TGTGAATGCC GCCTATGGGA ATCCCGTTCA GGCATCCAGC 
GTTTGTGGCG CCCAACAGCC GGAGCGTTAC TGCGAACTGT TGCGCGACGG AAATGCCGGC 
GAGTGTCGTT CATGCGAACA GCAGCGGTAC GGTCCGGCGG CATTAACCGA TCTAAATAAT 
CCCAGTAATG TGACCTGCTG GCGATCGGGT GCCGTTAATG TGCCCCACGA TCCGGACAGT 
GCGCCGCCGG ATAATGTGAC CCTAACCTTG TCGCTGGGCA AGAAGTACGA GCTGACCTAT 
ATATCGTTGT CATTTTGTCC CCGCTCGCCG CGTCCCGATT CGCTGGCCAT ATTCAAGAGC 
TCGGATTTTG GACAGACCTG GCAACCCTTC CAGTTCTATA GCTCACAGTG TCAAAAGTTC 
TATGGACGAC CGGATCGCGC TAAGATCTCC AAGTTTAATG AGCAGGAGGC CAGATGCATC 
AATTCGCAAC ATGACACGGG CGGAGCTGCT CAGCGATTTG CCTTCAATAC ATTGGAGGGT 
CGTCCGTCGG CCAATGATCT GGATTCCTCG CTGGTCCTGC AGGATTGGGT GACGGCCACG 
GATATAAGAG TTGTATTCCA TCGCCTTGAA TTGCCACCAC AATTGTTGAA GGTGAAAAAT 
GCAAATGCCT TTAGCGATGA GATGGGCGGC AGTCGCGAGG AGGATGAGGA TGATGATGCG 
GATCTGGAAC TGGATGGCGA GCAGGATGAG TACGACTACA ATCTGCAGGA CAACGACAGT 
GCCGATGCTG GGTACGATGA GTACGAGGAG CCCAAGAAGC ATTTGGAACT GGATGATGAT 
CATCTGCATT TGGACTATGC CAGTGATGGT GAATCGGTTG TAAAGAGGCA GGGCAAGCAC 
AAGGGTAGCG CCTATGAGAA GCACTACCAG AGCAAACTGG CGGCCACCAC GCCCCCACAA 
CAGCCGCCCA AGGTGACGCC ACCTGGCAAG GTCACGCCCC CTAGCACAGC TGCCCCTTCT 
GCCGCTGCCT CCGCGGTGAC CTTGCCCATT TCGCAGCATT ACGCCGTATC GGATTTTGCC 
GTCGGCGGAC GATGCAAGTG CAATGGTCAC GCCTCCGAGT GTGTGGCCAC CGTCAGTTCG 
GGATCAGGAA CGGCACTTTC GGATCAGGAC GATGGCCAGG ACGAGGACAC GCCGTCCGCC 
CCCTCGCTGG CCAACCACTT TGGTCGCAGT ACCCAGATGA GCGCCAAACT GACCATGACC 
TGCGCCTGCA AGCACAACAC CGCCGGACCG GAATGCGAAC GCTGCAAACC CTTCTACTTC 
GATCGACCCT GGGGCAGGGC CACCGACAAT GATGCCAACG AATGCAAAAT GTGCCAGTGC 
AACGGACACG CCCGTCGCTG CCGCTTCAAT CTGGAGCTGT ACAAACTATC TGGACGGGTT 
TCGGGAGGTG TATGCTACAA CTGCCAGCAC GATACTACCG GAAGATACTG CCACTATTGC 
CGCGAGGGAT ATTATCGGGA TGCCACTAAG CCGCCAAATC ATCGCAAAGT CTGCAAGCGT 
TGCGATTGCC ATCCAGTGGG CTCGACGGGC AAGACGTGCA ATCACCTGAG TGGCCAGTGT 
CCCTGCAAGG AGGGCGTCAC CGGACTAACT TGCAACCGAT GTGCCCGAGG CTATCAGCAG 
ACCCGCTCCC ACGTGGCACC CTGCATCAAG GTGCCCACTA ATGCGAACAT GATTCAGGCC 
GAGAGTGCTG GCGGCGGTGG AGGAGGTGGC ACTGGTGATT ACAAGGATGG CGGTGGCTCC 
CAGGTCGAGG AGATGAAAAA GTACTGCGGC AAGTGCAAGG CGAGTCCCAA GAAGCTCAAT 
CTCAACAAGT TTTGCATGGA AGATTATGCA ATTTTGGCCA AGGTGATTGG CCACGATCGC 
GCCTCCCAGG ACATCAGCAC GGAAAAGTTC TCGATAGAGC GACAGAACGA GATCTACAAG 
TACGAGATCA ATATCCAGAC GATCTTCAAG CGGAATCCCA TGTCGGGCAC TACGAGTTCC 
TTGCTCGGTA GGGGCAATAT GATGCTGCTG GTGCCGCGCA AGAGCATCGA GTGCCAGTGT 
CCAAAGATAA AGCTAAACAA ATCATATCTC ATCCTTGGAC GTGACTCGGA GGCGGCGCCT 
GGTTACTTGG CCATCGGACC GAGCTCGGTG GTCCTTGAAT GGAAGGACGA GTGGTCGCTT 
CGCATGAAGC GGTTCCAGCG GCGGGCACGC AAATGCAGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162755.1
CDS	321..2702
Translation	MVRATGTRMGLLLPIILALAIGSSAAGISSNDPCYFEGKPRKCLPSFVNAAYGNPVQASSVCGAQQPERYCELLRDGNAGECRSCEQQRYGPAALTDLNNPSNVTCWRSGAVNVPHDPDSAPPDNVTLTLSLGKKYELTYISLSFCPRSPRPDSLAIFKSSDFGQTWQPFQFYSSQCQKFYGRPDRAKISKFNEQEARCINSQHDTGGAAQRFAFNTLEGRPSANDLDSSLVLQDWVTATDIRVVFHRLELPPQLLKVKNANAFSDEMGGSREEDEDDDADLELDGEQDEYDYNLQDNDSADAGYDEYEEPKKHLELDDDHLHLDYASDGESVVKRQGKHKGSAYEKHYQSKLAATTPPQQPPKVTPPGKVTPPSTAAPSAAASAVTLPISQHYAVSDFAVGGRCKCNGHASECVATVSSGSGTALSDQDDGQDEDTPSAPSLANHFGRSTQMSAKLTMTCACKHNTAGPECERCKPFYFDRPWGRATDNDANECKMCQCNGHARRCRFNLELYKLSGRVSGGVCYNCQHDTTGRYCHYCREGYYRDATKPPNHRKVCKRCDCHPVGSTGKTCNHLSGQCPCKEGVTGLTCNRCARGYQQTRSHVAPCIKVPTNANMIQAESAGGGGGGGTGDYKDGGGSQVEEMKKYCGKCKASPKKLNLNKFCMEDYAILAKVIGHDRASQDISTEKFSIERQNEIYKYEINIQTIFKRNPMSGTTSSLLGRGNMMLLVPRKSIECQCPKIKLNKSYLILGRDSEAAPGYLAIGPSSVVLEWKDEWSLRMKRFQRRARKCS

Target ORF information:

RefSeq Version	NM_001169284.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Netrin-B (NetB), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169284.2

ORF Insert Sequence:

ATGGTCAGAG CGACAGGGAC AAGGATGGGC CTGCTCCTGC CCATAATCCT GGCACTGGCC 
ATCGGATCCT CGGCCGCCGG CATATCCTCC AACGATCCCT GCTACTTCGA GGGCAAGCCG 
CGCAAGTGCC TGCCAAGCTT TGTGAATGCC GCCTATGGGA ATCCCGTTCA GGCATCCAGC 
GTTTGTGGCG CCCAACAGCC GGAGCGTTAC TGCGAACTGT TGCGCGACGG AAATGCCGGC 
GAGTGTCGTT CATGCGAACA GCAGCGGTAC GGTCCGGCGG CATTAACCGA TCTAAATAAT 
CCCAGTAATG TGACCTGCTG GCGATCGGGT GCCGTTAATG TGCCCCACGA TCCGGACAGT 
GCGCCGCCGG ATAATGTGAC CCTAACCTTG TCGCTGGGCA AGAAGTACGA GCTGACCTAT 
ATATCGTTGT CATTTTGTCC CCGCTCGCCG CGTCCCGATT CGCTGGCCAT ATTCAAGAGC 
TCGGATTTTG GACAGACCTG GCAACCCTTC CAGTTCTATA GCTCACAGTG TCAAAAGTTC 
TATGGACGAC CGGATCGCGC TAAGATCTCC AAGTTTAATG AGCAGGAGGC CAGATGCATC 
AATTCGCAAC ATGACACGGG CGGAGCTGCT CAGCGATTTG CCTTCAATAC ATTGGAGGGT 
CGTCCGTCGG CCAATGATCT GGATTCCTCG CTGGTCCTGC AGGATTGGGT GACGGCCACG 
GATATAAGAG TTGTATTCCA TCGCCTTGAA TTGCCACCAC AATTGTTGAA GGTGAAAAAT 
GCAAATGCCT TTAGCGATGA GATGGGCGGC AGTCGCGAGG AGGATGAGGA TGATGATGCG 
GATCTGGAAC TGGATGGCGA GCAGGATGAG TACGACTACA ATCTGCAGGA CAACGACAGT 
GCCGATGCTG GGTACGATGA GTACGAGGAG CCCAAGAAGC ATTTGGAACT GGATGATGAT 
CATCTGCATT TGGACTATGC CAGTGATGGT GAATCGGTTG TAAAGAGGCA GGGCAAGCAC 
AAGGGTAGCG CCTATGAGAA GCACTACCAG AGCAAACTGG CGGCCACCAC GCCCCCACAA 
CAGCCGCCCA AGGTGACGCC ACCTGGCAAG GTCACGCCCC CTAGCACAGC TGCCCCTTCT 
GCCGCTGCCT CCGCGGTGAC CTTGCCCATT TCGCAGCATT ACGCCGTATC GGATTTTGCC 
GTCGGCGGAC GATGCAAGTG CAATGGTCAC GCCTCCGAGT GTGTGGCCAC CGTCAGTTCG 
GGATCAGGAA CGGCACTTTC GGATCAGGAC GATGGCCAGG ACGAGGACAC GCCGTCCGCC 
CCCTCGCTGG CCAACCACTT TGGTCGCAGT ACCCAGATGA GCGCCAAACT GACCATGACC 
TGCGCCTGCA AGCACAACAC CGCCGGACCG GAATGCGAAC GCTGCAAACC CTTCTACTTC 
GATCGACCCT GGGGCAGGGC CACCGACAAT GATGCCAACG AATGCAAAAT GTGCCAGTGC 
AACGGACACG CCCGTCGCTG CCGCTTCAAT CTGGAGCTGT ACAAACTATC TGGACGGGTT 
TCGGGAGGTG TATGCTACAA CTGCCAGCAC GATACTACCG GAAGATACTG CCACTATTGC 
CGCGAGGGAT ATTATCGGGA TGCCACTAAG CCGCCAAATC ATCGCAAAGT CTGCAAGCGT 
TGCGATTGCC ATCCAGTGGG CTCGACGGGC AAGACGTGCA ATCACCTGAG TGGCCAGTGT 
CCCTGCAAGG AGGGCGTCAC CGGACTAACT TGCAACCGAT GTGCCCGAGG CTATCAGCAG 
ACCCGCTCCC ACGTGGCACC CTGCATCAAG GTGCCCACTA ATGCGAACAT GATTCAGGCC 
GAGAGTGCTG GCGGCGGTGG AGGAGGTGGC ACTGGTGATT ACAAGGATGG CGGTGGCTCC 
CAGGTCGAGG AGATGAAAAA GTACTGCGGC AAGTGCAAGG CGAGTCCCAA GAAGCTCAAT 
CTCAACAAGT TTTGCATGGA AGATTATGCA ATTTTGGCCA AGGTGATTGG CCACGATCGC 
GCCTCCCAGG ACATCAGCAC GGAAAAGTTC TCGATAGAGC GACAGAACGA GATCTACAAG 
TACGAGATCA ATATCCAGAC GATCTTCAAG CGGAATCCCA TGTCGGGCAC TACGAGTTCC 
TTGCTCGGTA GGGGCAATAT GATGCTGCTG GTGCCGCGCA AGAGCATCGA GTGCCAGTGT 
CCAAAGATAA AGCTAAACAA ATCATATCTC ATCCTTGGAC GTGACTCGGA GGCGGCGCCT 
GGTTACTTGG CCATCGGACC GAGCTCGGTG GTCCTTGAAT GGAAGGACGA GTGGTCGCTT 
CGCATGAAGC GGTTCCAGCG GCGGGCACGC AAATGCAGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NetB ( NM_001258740.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245669.1 Drosophila melanogaster Netrin-B (NetB), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa01603
Clone ID Related Accession (Same CDS sequence)	NM_001169284.2 , NM_001258740.2 , NM_078600.4 , NM_001169282.1 , NM_001169283.2 , NM_001258739.2
Accession Version	NM_001258740.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2382bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Netrin-B, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_001258740.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTCAGAG CGACAGGGAC AAGGATGGGC CTGCTCCTGC CCATAATCCT GGCACTGGCC 
ATCGGATCCT CGGCCGCCGG CATATCCTCC AACGATCCCT GCTACTTCGA GGGCAAGCCG 
CGCAAGTGCC TGCCAAGCTT TGTGAATGCC GCCTATGGGA ATCCCGTTCA GGCATCCAGC 
GTTTGTGGCG CCCAACAGCC GGAGCGTTAC TGCGAACTGT TGCGCGACGG AAATGCCGGC 
GAGTGTCGTT CATGCGAACA GCAGCGGTAC GGTCCGGCGG CATTAACCGA TCTAAATAAT 
CCCAGTAATG TGACCTGCTG GCGATCGGGT GCCGTTAATG TGCCCCACGA TCCGGACAGT 
GCGCCGCCGG ATAATGTGAC CCTAACCTTG TCGCTGGGCA AGAAGTACGA GCTGACCTAT 
ATATCGTTGT CATTTTGTCC CCGCTCGCCG CGTCCCGATT CGCTGGCCAT ATTCAAGAGC 
TCGGATTTTG GACAGACCTG GCAACCCTTC CAGTTCTATA GCTCACAGTG TCAAAAGTTC 
TATGGACGAC CGGATCGCGC TAAGATCTCC AAGTTTAATG AGCAGGAGGC CAGATGCATC 
AATTCGCAAC ATGACACGGG CGGAGCTGCT CAGCGATTTG CCTTCAATAC ATTGGAGGGT 
CGTCCGTCGG CCAATGATCT GGATTCCTCG CTGGTCCTGC AGGATTGGGT GACGGCCACG 
GATATAAGAG TTGTATTCCA TCGCCTTGAA TTGCCACCAC AATTGTTGAA GGTGAAAAAT 
GCAAATGCCT TTAGCGATGA GATGGGCGGC AGTCGCGAGG AGGATGAGGA TGATGATGCG 
GATCTGGAAC TGGATGGCGA GCAGGATGAG TACGACTACA ATCTGCAGGA CAACGACAGT 
GCCGATGCTG GGTACGATGA GTACGAGGAG CCCAAGAAGC ATTTGGAACT GGATGATGAT 
CATCTGCATT TGGACTATGC CAGTGATGGT GAATCGGTTG TAAAGAGGCA GGGCAAGCAC 
AAGGGTAGCG CCTATGAGAA GCACTACCAG AGCAAACTGG CGGCCACCAC GCCCCCACAA 
CAGCCGCCCA AGGTGACGCC ACCTGGCAAG GTCACGCCCC CTAGCACAGC TGCCCCTTCT 
GCCGCTGCCT CCGCGGTGAC CTTGCCCATT TCGCAGCATT ACGCCGTATC GGATTTTGCC 
GTCGGCGGAC GATGCAAGTG CAATGGTCAC GCCTCCGAGT GTGTGGCCAC CGTCAGTTCG 
GGATCAGGAA CGGCACTTTC GGATCAGGAC GATGGCCAGG ACGAGGACAC GCCGTCCGCC 
CCCTCGCTGG CCAACCACTT TGGTCGCAGT ACCCAGATGA GCGCCAAACT GACCATGACC 
TGCGCCTGCA AGCACAACAC CGCCGGACCG GAATGCGAAC GCTGCAAACC CTTCTACTTC 
GATCGACCCT GGGGCAGGGC CACCGACAAT GATGCCAACG AATGCAAAAT GTGCCAGTGC 
AACGGACACG CCCGTCGCTG CCGCTTCAAT CTGGAGCTGT ACAAACTATC TGGACGGGTT 
TCGGGAGGTG TATGCTACAA CTGCCAGCAC GATACTACCG GAAGATACTG CCACTATTGC 
CGCGAGGGAT ATTATCGGGA TGCCACTAAG CCGCCAAATC ATCGCAAAGT CTGCAAGCGT 
TGCGATTGCC ATCCAGTGGG CTCGACGGGC AAGACGTGCA ATCACCTGAG TGGCCAGTGT 
CCCTGCAAGG AGGGCGTCAC CGGACTAACT TGCAACCGAT GTGCCCGAGG CTATCAGCAG 
ACCCGCTCCC ACGTGGCACC CTGCATCAAG GTGCCCACTA ATGCGAACAT GATTCAGGCC 
GAGAGTGCTG GCGGCGGTGG AGGAGGTGGC ACTGGTGATT ACAAGGATGG CGGTGGCTCC 
CAGGTCGAGG AGATGAAAAA GTACTGCGGC AAGTGCAAGG CGAGTCCCAA GAAGCTCAAT 
CTCAACAAGT TTTGCATGGA AGATTATGCA ATTTTGGCCA AGGTGATTGG CCACGATCGC 
GCCTCCCAGG ACATCAGCAC GGAAAAGTTC TCGATAGAGC GACAGAACGA GATCTACAAG 
TACGAGATCA ATATCCAGAC GATCTTCAAG CGGAATCCCA TGTCGGGCAC TACGAGTTCC 
TTGCTCGGTA GGGGCAATAT GATGCTGCTG GTGCCGCGCA AGAGCATCGA GTGCCAGTGT 
CCAAAGATAA AGCTAAACAA ATCATATCTC ATCCTTGGAC GTGACTCGGA GGCGGCGCCT 
GGTTACTTGG CCATCGGACC GAGCTCGGTG GTCCTTGAAT GGAAGGACGA GTGGTCGCTT 
CGCATGAAGC GGTTCCAGCG GCGGGCACGC AAATGCAGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245669.1
CDS	324..2705
Translation	MVRATGTRMGLLLPIILALAIGSSAAGISSNDPCYFEGKPRKCLPSFVNAAYGNPVQASSVCGAQQPERYCELLRDGNAGECRSCEQQRYGPAALTDLNNPSNVTCWRSGAVNVPHDPDSAPPDNVTLTLSLGKKYELTYISLSFCPRSPRPDSLAIFKSSDFGQTWQPFQFYSSQCQKFYGRPDRAKISKFNEQEARCINSQHDTGGAAQRFAFNTLEGRPSANDLDSSLVLQDWVTATDIRVVFHRLELPPQLLKVKNANAFSDEMGGSREEDEDDDADLELDGEQDEYDYNLQDNDSADAGYDEYEEPKKHLELDDDHLHLDYASDGESVVKRQGKHKGSAYEKHYQSKLAATTPPQQPPKVTPPGKVTPPSTAAPSAAASAVTLPISQHYAVSDFAVGGRCKCNGHASECVATVSSGSGTALSDQDDGQDEDTPSAPSLANHFGRSTQMSAKLTMTCACKHNTAGPECERCKPFYFDRPWGRATDNDANECKMCQCNGHARRCRFNLELYKLSGRVSGGVCYNCQHDTTGRYCHYCREGYYRDATKPPNHRKVCKRCDCHPVGSTGKTCNHLSGQCPCKEGVTGLTCNRCARGYQQTRSHVAPCIKVPTNANMIQAESAGGGGGGGTGDYKDGGGSQVEEMKKYCGKCKASPKKLNLNKFCMEDYAILAKVIGHDRASQDISTEKFSIERQNEIYKYEINIQTIFKRNPMSGTTSSLLGRGNMMLLVPRKSIECQCPKIKLNKSYLILGRDSEAAPGYLAIGPSSVVLEWKDEWSLRMKRFQRRARKCS

Target ORF information:

RefSeq Version	NM_001258740.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Netrin-B (NetB), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258740.2

ORF Insert Sequence:

ATGGTCAGAG CGACAGGGAC AAGGATGGGC CTGCTCCTGC CCATAATCCT GGCACTGGCC 
ATCGGATCCT CGGCCGCCGG CATATCCTCC AACGATCCCT GCTACTTCGA GGGCAAGCCG 
CGCAAGTGCC TGCCAAGCTT TGTGAATGCC GCCTATGGGA ATCCCGTTCA GGCATCCAGC 
GTTTGTGGCG CCCAACAGCC GGAGCGTTAC TGCGAACTGT TGCGCGACGG AAATGCCGGC 
GAGTGTCGTT CATGCGAACA GCAGCGGTAC GGTCCGGCGG CATTAACCGA TCTAAATAAT 
CCCAGTAATG TGACCTGCTG GCGATCGGGT GCCGTTAATG TGCCCCACGA TCCGGACAGT 
GCGCCGCCGG ATAATGTGAC CCTAACCTTG TCGCTGGGCA AGAAGTACGA GCTGACCTAT 
ATATCGTTGT CATTTTGTCC CCGCTCGCCG CGTCCCGATT CGCTGGCCAT ATTCAAGAGC 
TCGGATTTTG GACAGACCTG GCAACCCTTC CAGTTCTATA GCTCACAGTG TCAAAAGTTC 
TATGGACGAC CGGATCGCGC TAAGATCTCC AAGTTTAATG AGCAGGAGGC CAGATGCATC 
AATTCGCAAC ATGACACGGG CGGAGCTGCT CAGCGATTTG CCTTCAATAC ATTGGAGGGT 
CGTCCGTCGG CCAATGATCT GGATTCCTCG CTGGTCCTGC AGGATTGGGT GACGGCCACG 
GATATAAGAG TTGTATTCCA TCGCCTTGAA TTGCCACCAC AATTGTTGAA GGTGAAAAAT 
GCAAATGCCT TTAGCGATGA GATGGGCGGC AGTCGCGAGG AGGATGAGGA TGATGATGCG 
GATCTGGAAC TGGATGGCGA GCAGGATGAG TACGACTACA ATCTGCAGGA CAACGACAGT 
GCCGATGCTG GGTACGATGA GTACGAGGAG CCCAAGAAGC ATTTGGAACT GGATGATGAT 
CATCTGCATT TGGACTATGC CAGTGATGGT GAATCGGTTG TAAAGAGGCA GGGCAAGCAC 
AAGGGTAGCG CCTATGAGAA GCACTACCAG AGCAAACTGG CGGCCACCAC GCCCCCACAA 
CAGCCGCCCA AGGTGACGCC ACCTGGCAAG GTCACGCCCC CTAGCACAGC TGCCCCTTCT 
GCCGCTGCCT CCGCGGTGAC CTTGCCCATT TCGCAGCATT ACGCCGTATC GGATTTTGCC 
GTCGGCGGAC GATGCAAGTG CAATGGTCAC GCCTCCGAGT GTGTGGCCAC CGTCAGTTCG 
GGATCAGGAA CGGCACTTTC GGATCAGGAC GATGGCCAGG ACGAGGACAC GCCGTCCGCC 
CCCTCGCTGG CCAACCACTT TGGTCGCAGT ACCCAGATGA GCGCCAAACT GACCATGACC 
TGCGCCTGCA AGCACAACAC CGCCGGACCG GAATGCGAAC GCTGCAAACC CTTCTACTTC 
GATCGACCCT GGGGCAGGGC CACCGACAAT GATGCCAACG AATGCAAAAT GTGCCAGTGC 
AACGGACACG CCCGTCGCTG CCGCTTCAAT CTGGAGCTGT ACAAACTATC TGGACGGGTT 
TCGGGAGGTG TATGCTACAA CTGCCAGCAC GATACTACCG GAAGATACTG CCACTATTGC 
CGCGAGGGAT ATTATCGGGA TGCCACTAAG CCGCCAAATC ATCGCAAAGT CTGCAAGCGT 
TGCGATTGCC ATCCAGTGGG CTCGACGGGC AAGACGTGCA ATCACCTGAG TGGCCAGTGT 
CCCTGCAAGG AGGGCGTCAC CGGACTAACT TGCAACCGAT GTGCCCGAGG CTATCAGCAG 
ACCCGCTCCC ACGTGGCACC CTGCATCAAG GTGCCCACTA ATGCGAACAT GATTCAGGCC 
GAGAGTGCTG GCGGCGGTGG AGGAGGTGGC ACTGGTGATT ACAAGGATGG CGGTGGCTCC 
CAGGTCGAGG AGATGAAAAA GTACTGCGGC AAGTGCAAGG CGAGTCCCAA GAAGCTCAAT 
CTCAACAAGT TTTGCATGGA AGATTATGCA ATTTTGGCCA AGGTGATTGG CCACGATCGC 
GCCTCCCAGG ACATCAGCAC GGAAAAGTTC TCGATAGAGC GACAGAACGA GATCTACAAG 
TACGAGATCA ATATCCAGAC GATCTTCAAG CGGAATCCCA TGTCGGGCAC TACGAGTTCC 
TTGCTCGGTA GGGGCAATAT GATGCTGCTG GTGCCGCGCA AGAGCATCGA GTGCCAGTGT 
CCAAAGATAA AGCTAAACAA ATCATATCTC ATCCTTGGAC GTGACTCGGA GGCGGCGCCT 
GGTTACTTGG CCATCGGACC GAGCTCGGTG GTCCTTGAAT GGAAGGACGA GTGGTCGCTT 
CGCATGAAGC GGTTCCAGCG GCGGGCACGC AAATGCAGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NetB ( NM_078600.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_511155.1 Drosophila melanogaster Netrin-B (NetB), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa01603
Clone ID Related Accession (Same CDS sequence)	NM_001169284.2 , NM_001258740.2 , NM_078600.4 , NM_001169282.1 , NM_001169283.2 , NM_001258739.2
Accession Version	NM_078600.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2382bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Netrin-B, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_078600.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTCAGAG CGACAGGGAC AAGGATGGGC CTGCTCCTGC CCATAATCCT GGCACTGGCC 
ATCGGATCCT CGGCCGCCGG CATATCCTCC AACGATCCCT GCTACTTCGA GGGCAAGCCG 
CGCAAGTGCC TGCCAAGCTT TGTGAATGCC GCCTATGGGA ATCCCGTTCA GGCATCCAGC 
GTTTGTGGCG CCCAACAGCC GGAGCGTTAC TGCGAACTGT TGCGCGACGG AAATGCCGGC 
GAGTGTCGTT CATGCGAACA GCAGCGGTAC GGTCCGGCGG CATTAACCGA TCTAAATAAT 
CCCAGTAATG TGACCTGCTG GCGATCGGGT GCCGTTAATG TGCCCCACGA TCCGGACAGT 
GCGCCGCCGG ATAATGTGAC CCTAACCTTG TCGCTGGGCA AGAAGTACGA GCTGACCTAT 
ATATCGTTGT CATTTTGTCC CCGCTCGCCG CGTCCCGATT CGCTGGCCAT ATTCAAGAGC 
TCGGATTTTG GACAGACCTG GCAACCCTTC CAGTTCTATA GCTCACAGTG TCAAAAGTTC 
TATGGACGAC CGGATCGCGC TAAGATCTCC AAGTTTAATG AGCAGGAGGC CAGATGCATC 
AATTCGCAAC ATGACACGGG CGGAGCTGCT CAGCGATTTG CCTTCAATAC ATTGGAGGGT 
CGTCCGTCGG CCAATGATCT GGATTCCTCG CTGGTCCTGC AGGATTGGGT GACGGCCACG 
GATATAAGAG TTGTATTCCA TCGCCTTGAA TTGCCACCAC AATTGTTGAA GGTGAAAAAT 
GCAAATGCCT TTAGCGATGA GATGGGCGGC AGTCGCGAGG AGGATGAGGA TGATGATGCG 
GATCTGGAAC TGGATGGCGA GCAGGATGAG TACGACTACA ATCTGCAGGA CAACGACAGT 
GCCGATGCTG GGTACGATGA GTACGAGGAG CCCAAGAAGC ATTTGGAACT GGATGATGAT 
CATCTGCATT TGGACTATGC CAGTGATGGT GAATCGGTTG TAAAGAGGCA GGGCAAGCAC 
AAGGGTAGCG CCTATGAGAA GCACTACCAG AGCAAACTGG CGGCCACCAC GCCCCCACAA 
CAGCCGCCCA AGGTGACGCC ACCTGGCAAG GTCACGCCCC CTAGCACAGC TGCCCCTTCT 
GCCGCTGCCT CCGCGGTGAC CTTGCCCATT TCGCAGCATT ACGCCGTATC GGATTTTGCC 
GTCGGCGGAC GATGCAAGTG CAATGGTCAC GCCTCCGAGT GTGTGGCCAC CGTCAGTTCG 
GGATCAGGAA CGGCACTTTC GGATCAGGAC GATGGCCAGG ACGAGGACAC GCCGTCCGCC 
CCCTCGCTGG CCAACCACTT TGGTCGCAGT ACCCAGATGA GCGCCAAACT GACCATGACC 
TGCGCCTGCA AGCACAACAC CGCCGGACCG GAATGCGAAC GCTGCAAACC CTTCTACTTC 
GATCGACCCT GGGGCAGGGC CACCGACAAT GATGCCAACG AATGCAAAAT GTGCCAGTGC 
AACGGACACG CCCGTCGCTG CCGCTTCAAT CTGGAGCTGT ACAAACTATC TGGACGGGTT 
TCGGGAGGTG TATGCTACAA CTGCCAGCAC GATACTACCG GAAGATACTG CCACTATTGC 
CGCGAGGGAT ATTATCGGGA TGCCACTAAG CCGCCAAATC ATCGCAAAGT CTGCAAGCGT 
TGCGATTGCC ATCCAGTGGG CTCGACGGGC AAGACGTGCA ATCACCTGAG TGGCCAGTGT 
CCCTGCAAGG AGGGCGTCAC CGGACTAACT TGCAACCGAT GTGCCCGAGG CTATCAGCAG 
ACCCGCTCCC ACGTGGCACC CTGCATCAAG GTGCCCACTA ATGCGAACAT GATTCAGGCC 
GAGAGTGCTG GCGGCGGTGG AGGAGGTGGC ACTGGTGATT ACAAGGATGG CGGTGGCTCC 
CAGGTCGAGG AGATGAAAAA GTACTGCGGC AAGTGCAAGG CGAGTCCCAA GAAGCTCAAT 
CTCAACAAGT TTTGCATGGA AGATTATGCA ATTTTGGCCA AGGTGATTGG CCACGATCGC 
GCCTCCCAGG ACATCAGCAC GGAAAAGTTC TCGATAGAGC GACAGAACGA GATCTACAAG 
TACGAGATCA ATATCCAGAC GATCTTCAAG CGGAATCCCA TGTCGGGCAC TACGAGTTCC 
TTGCTCGGTA GGGGCAATAT GATGCTGCTG GTGCCGCGCA AGAGCATCGA GTGCCAGTGT 
CCAAAGATAA AGCTAAACAA ATCATATCTC ATCCTTGGAC GTGACTCGGA GGCGGCGCCT 
GGTTACTTGG CCATCGGACC GAGCTCGGTG GTCCTTGAAT GGAAGGACGA GTGGTCGCTT 
CGCATGAAGC GGTTCCAGCG GCGGGCACGC AAATGCAGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_511155.1
CDS	474..2855
Translation	MVRATGTRMGLLLPIILALAIGSSAAGISSNDPCYFEGKPRKCLPSFVNAAYGNPVQASSVCGAQQPERYCELLRDGNAGECRSCEQQRYGPAALTDLNNPSNVTCWRSGAVNVPHDPDSAPPDNVTLTLSLGKKYELTYISLSFCPRSPRPDSLAIFKSSDFGQTWQPFQFYSSQCQKFYGRPDRAKISKFNEQEARCINSQHDTGGAAQRFAFNTLEGRPSANDLDSSLVLQDWVTATDIRVVFHRLELPPQLLKVKNANAFSDEMGGSREEDEDDDADLELDGEQDEYDYNLQDNDSADAGYDEYEEPKKHLELDDDHLHLDYASDGESVVKRQGKHKGSAYEKHYQSKLAATTPPQQPPKVTPPGKVTPPSTAAPSAAASAVTLPISQHYAVSDFAVGGRCKCNGHASECVATVSSGSGTALSDQDDGQDEDTPSAPSLANHFGRSTQMSAKLTMTCACKHNTAGPECERCKPFYFDRPWGRATDNDANECKMCQCNGHARRCRFNLELYKLSGRVSGGVCYNCQHDTTGRYCHYCREGYYRDATKPPNHRKVCKRCDCHPVGSTGKTCNHLSGQCPCKEGVTGLTCNRCARGYQQTRSHVAPCIKVPTNANMIQAESAGGGGGGGTGDYKDGGGSQVEEMKKYCGKCKASPKKLNLNKFCMEDYAILAKVIGHDRASQDISTEKFSIERQNEIYKYEINIQTIFKRNPMSGTTSSLLGRGNMMLLVPRKSIECQCPKIKLNKSYLILGRDSEAAPGYLAIGPSSVVLEWKDEWSLRMKRFQRRARKCS

Target ORF information:

RefSeq Version	NM_078600.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Netrin-B (NetB), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078600.4

ORF Insert Sequence:

ATGGTCAGAG CGACAGGGAC AAGGATGGGC CTGCTCCTGC CCATAATCCT GGCACTGGCC 
ATCGGATCCT CGGCCGCCGG CATATCCTCC AACGATCCCT GCTACTTCGA GGGCAAGCCG 
CGCAAGTGCC TGCCAAGCTT TGTGAATGCC GCCTATGGGA ATCCCGTTCA GGCATCCAGC 
GTTTGTGGCG CCCAACAGCC GGAGCGTTAC TGCGAACTGT TGCGCGACGG AAATGCCGGC 
GAGTGTCGTT CATGCGAACA GCAGCGGTAC GGTCCGGCGG CATTAACCGA TCTAAATAAT 
CCCAGTAATG TGACCTGCTG GCGATCGGGT GCCGTTAATG TGCCCCACGA TCCGGACAGT 
GCGCCGCCGG ATAATGTGAC CCTAACCTTG TCGCTGGGCA AGAAGTACGA GCTGACCTAT 
ATATCGTTGT CATTTTGTCC CCGCTCGCCG CGTCCCGATT CGCTGGCCAT ATTCAAGAGC 
TCGGATTTTG GACAGACCTG GCAACCCTTC CAGTTCTATA GCTCACAGTG TCAAAAGTTC 
TATGGACGAC CGGATCGCGC TAAGATCTCC AAGTTTAATG AGCAGGAGGC CAGATGCATC 
AATTCGCAAC ATGACACGGG CGGAGCTGCT CAGCGATTTG CCTTCAATAC ATTGGAGGGT 
CGTCCGTCGG CCAATGATCT GGATTCCTCG CTGGTCCTGC AGGATTGGGT GACGGCCACG 
GATATAAGAG TTGTATTCCA TCGCCTTGAA TTGCCACCAC AATTGTTGAA GGTGAAAAAT 
GCAAATGCCT TTAGCGATGA GATGGGCGGC AGTCGCGAGG AGGATGAGGA TGATGATGCG 
GATCTGGAAC TGGATGGCGA GCAGGATGAG TACGACTACA ATCTGCAGGA CAACGACAGT 
GCCGATGCTG GGTACGATGA GTACGAGGAG CCCAAGAAGC ATTTGGAACT GGATGATGAT 
CATCTGCATT TGGACTATGC CAGTGATGGT GAATCGGTTG TAAAGAGGCA GGGCAAGCAC 
AAGGGTAGCG CCTATGAGAA GCACTACCAG AGCAAACTGG CGGCCACCAC GCCCCCACAA 
CAGCCGCCCA AGGTGACGCC ACCTGGCAAG GTCACGCCCC CTAGCACAGC TGCCCCTTCT 
GCCGCTGCCT CCGCGGTGAC CTTGCCCATT TCGCAGCATT ACGCCGTATC GGATTTTGCC 
GTCGGCGGAC GATGCAAGTG CAATGGTCAC GCCTCCGAGT GTGTGGCCAC CGTCAGTTCG 
GGATCAGGAA CGGCACTTTC GGATCAGGAC GATGGCCAGG ACGAGGACAC GCCGTCCGCC 
CCCTCGCTGG CCAACCACTT TGGTCGCAGT ACCCAGATGA GCGCCAAACT GACCATGACC 
TGCGCCTGCA AGCACAACAC CGCCGGACCG GAATGCGAAC GCTGCAAACC CTTCTACTTC 
GATCGACCCT GGGGCAGGGC CACCGACAAT GATGCCAACG AATGCAAAAT GTGCCAGTGC 
AACGGACACG CCCGTCGCTG CCGCTTCAAT CTGGAGCTGT ACAAACTATC TGGACGGGTT 
TCGGGAGGTG TATGCTACAA CTGCCAGCAC GATACTACCG GAAGATACTG CCACTATTGC 
CGCGAGGGAT ATTATCGGGA TGCCACTAAG CCGCCAAATC ATCGCAAAGT CTGCAAGCGT 
TGCGATTGCC ATCCAGTGGG CTCGACGGGC AAGACGTGCA ATCACCTGAG TGGCCAGTGT 
CCCTGCAAGG AGGGCGTCAC CGGACTAACT TGCAACCGAT GTGCCCGAGG CTATCAGCAG 
ACCCGCTCCC ACGTGGCACC CTGCATCAAG GTGCCCACTA ATGCGAACAT GATTCAGGCC 
GAGAGTGCTG GCGGCGGTGG AGGAGGTGGC ACTGGTGATT ACAAGGATGG CGGTGGCTCC 
CAGGTCGAGG AGATGAAAAA GTACTGCGGC AAGTGCAAGG CGAGTCCCAA GAAGCTCAAT 
CTCAACAAGT TTTGCATGGA AGATTATGCA ATTTTGGCCA AGGTGATTGG CCACGATCGC 
GCCTCCCAGG ACATCAGCAC GGAAAAGTTC TCGATAGAGC GACAGAACGA GATCTACAAG 
TACGAGATCA ATATCCAGAC GATCTTCAAG CGGAATCCCA TGTCGGGCAC TACGAGTTCC 
TTGCTCGGTA GGGGCAATAT GATGCTGCTG GTGCCGCGCA AGAGCATCGA GTGCCAGTGT 
CCAAAGATAA AGCTAAACAA ATCATATCTC ATCCTTGGAC GTGACTCGGA GGCGGCGCCT 
GGTTACTTGG CCATCGGACC GAGCTCGGTG GTCCTTGAAT GGAAGGACGA GTGGTCGCTT 
CGCATGAAGC GGTTCCAGCG GCGGGCACGC AAATGCAGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NetB ( NM_001169282.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001162753.1 Drosophila melanogaster Netrin-B (NetB), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa01603
Clone ID Related Accession (Same CDS sequence)	NM_001169284.2 , NM_001258740.2 , NM_078600.4 , NM_001169282.1 , NM_001169283.2 , NM_001258739.2
Accession Version	NM_001169282.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2382bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Netrin-B, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTCAGAG CGACAGGGAC AAGGATGGGC CTGCTCCTGC CCATAATCCT GGCACTGGCC 
ATCGGATCCT CGGCCGCCGG CATATCCTCC AACGATCCCT GCTACTTCGA GGGCAAGCCG 
CGCAAGTGCC TGCCAAGCTT TGTGAATGCC GCCTATGGGA ATCCCGTTCA GGCATCCAGC 
GTTTGTGGCG CCCAACAGCC GGAGCGTTAC TGCGAACTGT TGCGCGACGG AAATGCCGGC 
GAGTGTCGTT CATGCGAACA GCAGCGGTAC GGTCCGGCGG CATTAACCGA TCTAAATAAT 
CCCAGTAATG TGACCTGCTG GCGATCGGGT GCCGTTAATG TGCCCCACGA TCCGGACAGT 
GCGCCGCCGG ATAATGTGAC CCTAACCTTG TCGCTGGGCA AGAAGTACGA GCTGACCTAT 
ATATCGTTGT CATTTTGTCC CCGCTCGCCG CGTCCCGATT CGCTGGCCAT ATTCAAGAGC 
TCGGATTTTG GACAGACCTG GCAACCCTTC CAGTTCTATA GCTCACAGTG TCAAAAGTTC 
TATGGACGAC CGGATCGCGC TAAGATCTCC AAGTTTAATG AGCAGGAGGC CAGATGCATC 
AATTCGCAAC ATGACACGGG CGGAGCTGCT CAGCGATTTG CCTTCAATAC ATTGGAGGGT 
CGTCCGTCGG CCAATGATCT GGATTCCTCG CTGGTCCTGC AGGATTGGGT GACGGCCACG 
GATATAAGAG TTGTATTCCA TCGCCTTGAA TTGCCACCAC AATTGTTGAA GGTGAAAAAT 
GCAAATGCCT TTAGCGATGA GATGGGCGGC AGTCGCGAGG AGGATGAGGA TGATGATGCG 
GATCTGGAAC TGGATGGCGA GCAGGATGAG TACGACTACA ATCTGCAGGA CAACGACAGT 
GCCGATGCTG GGTACGATGA GTACGAGGAG CCCAAGAAGC ATTTGGAACT GGATGATGAT 
CATCTGCATT TGGACTATGC CAGTGATGGT GAATCGGTTG TAAAGAGGCA GGGCAAGCAC 
AAGGGTAGCG CCTATGAGAA GCACTACCAG AGCAAACTGG CGGCCACCAC GCCCCCACAA 
CAGCCGCCCA AGGTGACGCC ACCTGGCAAG GTCACGCCCC CTAGCACAGC TGCCCCTTCT 
GCCGCTGCCT CCGCGGTGAC CTTGCCCATT TCGCAGCATT ACGCCGTATC GGATTTTGCC 
GTCGGCGGAC GATGCAAGTG CAATGGTCAC GCCTCCGAGT GTGTGGCCAC CGTCAGTTCG 
GGATCAGGAA CGGCACTTTC GGATCAGGAC GATGGCCAGG ACGAGGACAC GCCGTCCGCC 
CCCTCGCTGG CCAACCACTT TGGTCGCAGT ACCCAGATGA GCGCCAAACT GACCATGACC 
TGCGCCTGCA AGCACAACAC CGCCGGACCG GAATGCGAAC GCTGCAAACC CTTCTACTTC 
GATCGACCCT GGGGCAGGGC CACCGACAAT GATGCCAACG AATGCAAAAT GTGCCAGTGC 
AACGGACACG CCCGTCGCTG CCGCTTCAAT CTGGAGCTGT ACAAACTATC TGGACGGGTT 
TCGGGAGGTG TATGCTACAA CTGCCAGCAC GATACTACCG GAAGATACTG CCACTATTGC 
CGCGAGGGAT ATTATCGGGA TGCCACTAAG CCGCCAAATC ATCGCAAAGT CTGCAAGCGT 
TGCGATTGCC ATCCAGTGGG CTCGACGGGC AAGACGTGCA ATCACCTGAG TGGCCAGTGT 
CCCTGCAAGG AGGGCGTCAC CGGACTAACT TGCAACCGAT GTGCCCGAGG CTATCAGCAG 
ACCCGCTCCC ACGTGGCACC CTGCATCAAG GTGCCCACTA ATGCGAACAT GATTCAGGCC 
GAGAGTGCTG GCGGCGGTGG AGGAGGTGGC ACTGGTGATT ACAAGGATGG CGGTGGCTCC 
CAGGTCGAGG AGATGAAAAA GTACTGCGGC AAGTGCAAGG CGAGTCCCAA GAAGCTCAAT 
CTCAACAAGT TTTGCATGGA AGATTATGCA ATTTTGGCCA AGGTGATTGG CCACGATCGC 
GCCTCCCAGG ACATCAGCAC GGAAAAGTTC TCGATAGAGC GACAGAACGA GATCTACAAG 
TACGAGATCA ATATCCAGAC GATCTTCAAG CGGAATCCCA TGTCGGGCAC TACGAGTTCC 
TTGCTCGGTA GGGGCAATAT GATGCTGCTG GTGCCGCGCA AGAGCATCGA GTGCCAGTGT 
CCAAAGATAA AGCTAAACAA ATCATATCTC ATCCTTGGAC GTGACTCGGA GGCGGCGCCT 
GGTTACTTGG CCATCGGACC GAGCTCGGTG GTCCTTGAAT GGAAGGACGA GTGGTCGCTT 
CGCATGAAGC GGTTCCAGCG GCGGGCACGC AAATGCAGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162753.1
CDS	381..2762
Translation	MVRATGTRMGLLLPIILALAIGSSAAGISSNDPCYFEGKPRKCLPSFVNAAYGNPVQASSVCGAQQPERYCELLRDGNAGECRSCEQQRYGPAALTDLNNPSNVTCWRSGAVNVPHDPDSAPPDNVTLTLSLGKKYELTYISLSFCPRSPRPDSLAIFKSSDFGQTWQPFQFYSSQCQKFYGRPDRAKISKFNEQEARCINSQHDTGGAAQRFAFNTLEGRPSANDLDSSLVLQDWVTATDIRVVFHRLELPPQLLKVKNANAFSDEMGGSREEDEDDDADLELDGEQDEYDYNLQDNDSADAGYDEYEEPKKHLELDDDHLHLDYASDGESVVKRQGKHKGSAYEKHYQSKLAATTPPQQPPKVTPPGKVTPPSTAAPSAAASAVTLPISQHYAVSDFAVGGRCKCNGHASECVATVSSGSGTALSDQDDGQDEDTPSAPSLANHFGRSTQMSAKLTMTCACKHNTAGPECERCKPFYFDRPWGRATDNDANECKMCQCNGHARRCRFNLELYKLSGRVSGGVCYNCQHDTTGRYCHYCREGYYRDATKPPNHRKVCKRCDCHPVGSTGKTCNHLSGQCPCKEGVTGLTCNRCARGYQQTRSHVAPCIKVPTNANMIQAESAGGGGGGGTGDYKDGGGSQVEEMKKYCGKCKASPKKLNLNKFCMEDYAILAKVIGHDRASQDISTEKFSIERQNEIYKYEINIQTIFKRNPMSGTTSSLLGRGNMMLLVPRKSIECQCPKIKLNKSYLILGRDSEAAPGYLAIGPSSVVLEWKDEWSLRMKRFQRRARKCS

Target ORF information:

RefSeq Version	NM_001169282.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Netrin-B (NetB), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169282.1

ORF Insert Sequence:

ATGGTCAGAG CGACAGGGAC AAGGATGGGC CTGCTCCTGC CCATAATCCT GGCACTGGCC 
ATCGGATCCT CGGCCGCCGG CATATCCTCC AACGATCCCT GCTACTTCGA GGGCAAGCCG 
CGCAAGTGCC TGCCAAGCTT TGTGAATGCC GCCTATGGGA ATCCCGTTCA GGCATCCAGC 
GTTTGTGGCG CCCAACAGCC GGAGCGTTAC TGCGAACTGT TGCGCGACGG AAATGCCGGC 
GAGTGTCGTT CATGCGAACA GCAGCGGTAC GGTCCGGCGG CATTAACCGA TCTAAATAAT 
CCCAGTAATG TGACCTGCTG GCGATCGGGT GCCGTTAATG TGCCCCACGA TCCGGACAGT 
GCGCCGCCGG ATAATGTGAC CCTAACCTTG TCGCTGGGCA AGAAGTACGA GCTGACCTAT 
ATATCGTTGT CATTTTGTCC CCGCTCGCCG CGTCCCGATT CGCTGGCCAT ATTCAAGAGC 
TCGGATTTTG GACAGACCTG GCAACCCTTC CAGTTCTATA GCTCACAGTG TCAAAAGTTC 
TATGGACGAC CGGATCGCGC TAAGATCTCC AAGTTTAATG AGCAGGAGGC CAGATGCATC 
AATTCGCAAC ATGACACGGG CGGAGCTGCT CAGCGATTTG CCTTCAATAC ATTGGAGGGT 
CGTCCGTCGG CCAATGATCT GGATTCCTCG CTGGTCCTGC AGGATTGGGT GACGGCCACG 
GATATAAGAG TTGTATTCCA TCGCCTTGAA TTGCCACCAC AATTGTTGAA GGTGAAAAAT 
GCAAATGCCT TTAGCGATGA GATGGGCGGC AGTCGCGAGG AGGATGAGGA TGATGATGCG 
GATCTGGAAC TGGATGGCGA GCAGGATGAG TACGACTACA ATCTGCAGGA CAACGACAGT 
GCCGATGCTG GGTACGATGA GTACGAGGAG CCCAAGAAGC ATTTGGAACT GGATGATGAT 
CATCTGCATT TGGACTATGC CAGTGATGGT GAATCGGTTG TAAAGAGGCA GGGCAAGCAC 
AAGGGTAGCG CCTATGAGAA GCACTACCAG AGCAAACTGG CGGCCACCAC GCCCCCACAA 
CAGCCGCCCA AGGTGACGCC ACCTGGCAAG GTCACGCCCC CTAGCACAGC TGCCCCTTCT 
GCCGCTGCCT CCGCGGTGAC CTTGCCCATT TCGCAGCATT ACGCCGTATC GGATTTTGCC 
GTCGGCGGAC GATGCAAGTG CAATGGTCAC GCCTCCGAGT GTGTGGCCAC CGTCAGTTCG 
GGATCAGGAA CGGCACTTTC GGATCAGGAC GATGGCCAGG ACGAGGACAC GCCGTCCGCC 
CCCTCGCTGG CCAACCACTT TGGTCGCAGT ACCCAGATGA GCGCCAAACT GACCATGACC 
TGCGCCTGCA AGCACAACAC CGCCGGACCG GAATGCGAAC GCTGCAAACC CTTCTACTTC 
GATCGACCCT GGGGCAGGGC CACCGACAAT GATGCCAACG AATGCAAAAT GTGCCAGTGC 
AACGGACACG CCCGTCGCTG CCGCTTCAAT CTGGAGCTGT ACAAACTATC TGGACGGGTT 
TCGGGAGGTG TATGCTACAA CTGCCAGCAC GATACTACCG GAAGATACTG CCACTATTGC 
CGCGAGGGAT ATTATCGGGA TGCCACTAAG CCGCCAAATC ATCGCAAAGT CTGCAAGCGT 
TGCGATTGCC ATCCAGTGGG CTCGACGGGC AAGACGTGCA ATCACCTGAG TGGCCAGTGT 
CCCTGCAAGG AGGGCGTCAC CGGACTAACT TGCAACCGAT GTGCCCGAGG CTATCAGCAG 
ACCCGCTCCC ACGTGGCACC CTGCATCAAG GTGCCCACTA ATGCGAACAT GATTCAGGCC 
GAGAGTGCTG GCGGCGGTGG AGGAGGTGGC ACTGGTGATT ACAAGGATGG CGGTGGCTCC 
CAGGTCGAGG AGATGAAAAA GTACTGCGGC AAGTGCAAGG CGAGTCCCAA GAAGCTCAAT 
CTCAACAAGT TTTGCATGGA AGATTATGCA ATTTTGGCCA AGGTGATTGG CCACGATCGC 
GCCTCCCAGG ACATCAGCAC GGAAAAGTTC TCGATAGAGC GACAGAACGA GATCTACAAG 
TACGAGATCA ATATCCAGAC GATCTTCAAG CGGAATCCCA TGTCGGGCAC TACGAGTTCC 
TTGCTCGGTA GGGGCAATAT GATGCTGCTG GTGCCGCGCA AGAGCATCGA GTGCCAGTGT 
CCAAAGATAA AGCTAAACAA ATCATATCTC ATCCTTGGAC GTGACTCGGA GGCGGCGCCT 
GGTTACTTGG CCATCGGACC GAGCTCGGTG GTCCTTGAAT GGAAGGACGA GTGGTCGCTT 
CGCATGAAGC GGTTCCAGCG GCGGGCACGC AAATGCAGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NetB ( NM_001169283.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001162754.1 Drosophila melanogaster Netrin-B (NetB), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa01603
Clone ID Related Accession (Same CDS sequence)	NM_001169284.2 , NM_001258740.2 , NM_078600.4 , NM_001169282.1 , NM_001169283.2 , NM_001258739.2
Accession Version	NM_001169283.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2382bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Netrin-B, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_001169283.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTCAGAG CGACAGGGAC AAGGATGGGC CTGCTCCTGC CCATAATCCT GGCACTGGCC 
ATCGGATCCT CGGCCGCCGG CATATCCTCC AACGATCCCT GCTACTTCGA GGGCAAGCCG 
CGCAAGTGCC TGCCAAGCTT TGTGAATGCC GCCTATGGGA ATCCCGTTCA GGCATCCAGC 
GTTTGTGGCG CCCAACAGCC GGAGCGTTAC TGCGAACTGT TGCGCGACGG AAATGCCGGC 
GAGTGTCGTT CATGCGAACA GCAGCGGTAC GGTCCGGCGG CATTAACCGA TCTAAATAAT 
CCCAGTAATG TGACCTGCTG GCGATCGGGT GCCGTTAATG TGCCCCACGA TCCGGACAGT 
GCGCCGCCGG ATAATGTGAC CCTAACCTTG TCGCTGGGCA AGAAGTACGA GCTGACCTAT 
ATATCGTTGT CATTTTGTCC CCGCTCGCCG CGTCCCGATT CGCTGGCCAT ATTCAAGAGC 
TCGGATTTTG GACAGACCTG GCAACCCTTC CAGTTCTATA GCTCACAGTG TCAAAAGTTC 
TATGGACGAC CGGATCGCGC TAAGATCTCC AAGTTTAATG AGCAGGAGGC CAGATGCATC 
AATTCGCAAC ATGACACGGG CGGAGCTGCT CAGCGATTTG CCTTCAATAC ATTGGAGGGT 
CGTCCGTCGG CCAATGATCT GGATTCCTCG CTGGTCCTGC AGGATTGGGT GACGGCCACG 
GATATAAGAG TTGTATTCCA TCGCCTTGAA TTGCCACCAC AATTGTTGAA GGTGAAAAAT 
GCAAATGCCT TTAGCGATGA GATGGGCGGC AGTCGCGAGG AGGATGAGGA TGATGATGCG 
GATCTGGAAC TGGATGGCGA GCAGGATGAG TACGACTACA ATCTGCAGGA CAACGACAGT 
GCCGATGCTG GGTACGATGA GTACGAGGAG CCCAAGAAGC ATTTGGAACT GGATGATGAT 
CATCTGCATT TGGACTATGC CAGTGATGGT GAATCGGTTG TAAAGAGGCA GGGCAAGCAC 
AAGGGTAGCG CCTATGAGAA GCACTACCAG AGCAAACTGG CGGCCACCAC GCCCCCACAA 
CAGCCGCCCA AGGTGACGCC ACCTGGCAAG GTCACGCCCC CTAGCACAGC TGCCCCTTCT 
GCCGCTGCCT CCGCGGTGAC CTTGCCCATT TCGCAGCATT ACGCCGTATC GGATTTTGCC 
GTCGGCGGAC GATGCAAGTG CAATGGTCAC GCCTCCGAGT GTGTGGCCAC CGTCAGTTCG 
GGATCAGGAA CGGCACTTTC GGATCAGGAC GATGGCCAGG ACGAGGACAC GCCGTCCGCC 
CCCTCGCTGG CCAACCACTT TGGTCGCAGT ACCCAGATGA GCGCCAAACT GACCATGACC 
TGCGCCTGCA AGCACAACAC CGCCGGACCG GAATGCGAAC GCTGCAAACC CTTCTACTTC 
GATCGACCCT GGGGCAGGGC CACCGACAAT GATGCCAACG AATGCAAAAT GTGCCAGTGC 
AACGGACACG CCCGTCGCTG CCGCTTCAAT CTGGAGCTGT ACAAACTATC TGGACGGGTT 
TCGGGAGGTG TATGCTACAA CTGCCAGCAC GATACTACCG GAAGATACTG CCACTATTGC 
CGCGAGGGAT ATTATCGGGA TGCCACTAAG CCGCCAAATC ATCGCAAAGT CTGCAAGCGT 
TGCGATTGCC ATCCAGTGGG CTCGACGGGC AAGACGTGCA ATCACCTGAG TGGCCAGTGT 
CCCTGCAAGG AGGGCGTCAC CGGACTAACT TGCAACCGAT GTGCCCGAGG CTATCAGCAG 
ACCCGCTCCC ACGTGGCACC CTGCATCAAG GTGCCCACTA ATGCGAACAT GATTCAGGCC 
GAGAGTGCTG GCGGCGGTGG AGGAGGTGGC ACTGGTGATT ACAAGGATGG CGGTGGCTCC 
CAGGTCGAGG AGATGAAAAA GTACTGCGGC AAGTGCAAGG CGAGTCCCAA GAAGCTCAAT 
CTCAACAAGT TTTGCATGGA AGATTATGCA ATTTTGGCCA AGGTGATTGG CCACGATCGC 
GCCTCCCAGG ACATCAGCAC GGAAAAGTTC TCGATAGAGC GACAGAACGA GATCTACAAG 
TACGAGATCA ATATCCAGAC GATCTTCAAG CGGAATCCCA TGTCGGGCAC TACGAGTTCC 
TTGCTCGGTA GGGGCAATAT GATGCTGCTG GTGCCGCGCA AGAGCATCGA GTGCCAGTGT 
CCAAAGATAA AGCTAAACAA ATCATATCTC ATCCTTGGAC GTGACTCGGA GGCGGCGCCT 
GGTTACTTGG CCATCGGACC GAGCTCGGTG GTCCTTGAAT GGAAGGACGA GTGGTCGCTT 
CGCATGAAGC GGTTCCAGCG GCGGGCACGC AAATGCAGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162754.1
CDS	384..2765
Translation	MVRATGTRMGLLLPIILALAIGSSAAGISSNDPCYFEGKPRKCLPSFVNAAYGNPVQASSVCGAQQPERYCELLRDGNAGECRSCEQQRYGPAALTDLNNPSNVTCWRSGAVNVPHDPDSAPPDNVTLTLSLGKKYELTYISLSFCPRSPRPDSLAIFKSSDFGQTWQPFQFYSSQCQKFYGRPDRAKISKFNEQEARCINSQHDTGGAAQRFAFNTLEGRPSANDLDSSLVLQDWVTATDIRVVFHRLELPPQLLKVKNANAFSDEMGGSREEDEDDDADLELDGEQDEYDYNLQDNDSADAGYDEYEEPKKHLELDDDHLHLDYASDGESVVKRQGKHKGSAYEKHYQSKLAATTPPQQPPKVTPPGKVTPPSTAAPSAAASAVTLPISQHYAVSDFAVGGRCKCNGHASECVATVSSGSGTALSDQDDGQDEDTPSAPSLANHFGRSTQMSAKLTMTCACKHNTAGPECERCKPFYFDRPWGRATDNDANECKMCQCNGHARRCRFNLELYKLSGRVSGGVCYNCQHDTTGRYCHYCREGYYRDATKPPNHRKVCKRCDCHPVGSTGKTCNHLSGQCPCKEGVTGLTCNRCARGYQQTRSHVAPCIKVPTNANMIQAESAGGGGGGGTGDYKDGGGSQVEEMKKYCGKCKASPKKLNLNKFCMEDYAILAKVIGHDRASQDISTEKFSIERQNEIYKYEINIQTIFKRNPMSGTTSSLLGRGNMMLLVPRKSIECQCPKIKLNKSYLILGRDSEAAPGYLAIGPSSVVLEWKDEWSLRMKRFQRRARKCS

Target ORF information:

RefSeq Version	NM_001169283.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Netrin-B (NetB), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169283.2

ORF Insert Sequence:

ATGGTCAGAG CGACAGGGAC AAGGATGGGC CTGCTCCTGC CCATAATCCT GGCACTGGCC 
ATCGGATCCT CGGCCGCCGG CATATCCTCC AACGATCCCT GCTACTTCGA GGGCAAGCCG 
CGCAAGTGCC TGCCAAGCTT TGTGAATGCC GCCTATGGGA ATCCCGTTCA GGCATCCAGC 
GTTTGTGGCG CCCAACAGCC GGAGCGTTAC TGCGAACTGT TGCGCGACGG AAATGCCGGC 
GAGTGTCGTT CATGCGAACA GCAGCGGTAC GGTCCGGCGG CATTAACCGA TCTAAATAAT 
CCCAGTAATG TGACCTGCTG GCGATCGGGT GCCGTTAATG TGCCCCACGA TCCGGACAGT 
GCGCCGCCGG ATAATGTGAC CCTAACCTTG TCGCTGGGCA AGAAGTACGA GCTGACCTAT 
ATATCGTTGT CATTTTGTCC CCGCTCGCCG CGTCCCGATT CGCTGGCCAT ATTCAAGAGC 
TCGGATTTTG GACAGACCTG GCAACCCTTC CAGTTCTATA GCTCACAGTG TCAAAAGTTC 
TATGGACGAC CGGATCGCGC TAAGATCTCC AAGTTTAATG AGCAGGAGGC CAGATGCATC 
AATTCGCAAC ATGACACGGG CGGAGCTGCT CAGCGATTTG CCTTCAATAC ATTGGAGGGT 
CGTCCGTCGG CCAATGATCT GGATTCCTCG CTGGTCCTGC AGGATTGGGT GACGGCCACG 
GATATAAGAG TTGTATTCCA TCGCCTTGAA TTGCCACCAC AATTGTTGAA GGTGAAAAAT 
GCAAATGCCT TTAGCGATGA GATGGGCGGC AGTCGCGAGG AGGATGAGGA TGATGATGCG 
GATCTGGAAC TGGATGGCGA GCAGGATGAG TACGACTACA ATCTGCAGGA CAACGACAGT 
GCCGATGCTG GGTACGATGA GTACGAGGAG CCCAAGAAGC ATTTGGAACT GGATGATGAT 
CATCTGCATT TGGACTATGC CAGTGATGGT GAATCGGTTG TAAAGAGGCA GGGCAAGCAC 
AAGGGTAGCG CCTATGAGAA GCACTACCAG AGCAAACTGG CGGCCACCAC GCCCCCACAA 
CAGCCGCCCA AGGTGACGCC ACCTGGCAAG GTCACGCCCC CTAGCACAGC TGCCCCTTCT 
GCCGCTGCCT CCGCGGTGAC CTTGCCCATT TCGCAGCATT ACGCCGTATC GGATTTTGCC 
GTCGGCGGAC GATGCAAGTG CAATGGTCAC GCCTCCGAGT GTGTGGCCAC CGTCAGTTCG 
GGATCAGGAA CGGCACTTTC GGATCAGGAC GATGGCCAGG ACGAGGACAC GCCGTCCGCC 
CCCTCGCTGG CCAACCACTT TGGTCGCAGT ACCCAGATGA GCGCCAAACT GACCATGACC 
TGCGCCTGCA AGCACAACAC CGCCGGACCG GAATGCGAAC GCTGCAAACC CTTCTACTTC 
GATCGACCCT GGGGCAGGGC CACCGACAAT GATGCCAACG AATGCAAAAT GTGCCAGTGC 
AACGGACACG CCCGTCGCTG CCGCTTCAAT CTGGAGCTGT ACAAACTATC TGGACGGGTT 
TCGGGAGGTG TATGCTACAA CTGCCAGCAC GATACTACCG GAAGATACTG CCACTATTGC 
CGCGAGGGAT ATTATCGGGA TGCCACTAAG CCGCCAAATC ATCGCAAAGT CTGCAAGCGT 
TGCGATTGCC ATCCAGTGGG CTCGACGGGC AAGACGTGCA ATCACCTGAG TGGCCAGTGT 
CCCTGCAAGG AGGGCGTCAC CGGACTAACT TGCAACCGAT GTGCCCGAGG CTATCAGCAG 
ACCCGCTCCC ACGTGGCACC CTGCATCAAG GTGCCCACTA ATGCGAACAT GATTCAGGCC 
GAGAGTGCTG GCGGCGGTGG AGGAGGTGGC ACTGGTGATT ACAAGGATGG CGGTGGCTCC 
CAGGTCGAGG AGATGAAAAA GTACTGCGGC AAGTGCAAGG CGAGTCCCAA GAAGCTCAAT 
CTCAACAAGT TTTGCATGGA AGATTATGCA ATTTTGGCCA AGGTGATTGG CCACGATCGC 
GCCTCCCAGG ACATCAGCAC GGAAAAGTTC TCGATAGAGC GACAGAACGA GATCTACAAG 
TACGAGATCA ATATCCAGAC GATCTTCAAG CGGAATCCCA TGTCGGGCAC TACGAGTTCC 
TTGCTCGGTA GGGGCAATAT GATGCTGCTG GTGCCGCGCA AGAGCATCGA GTGCCAGTGT 
CCAAAGATAA AGCTAAACAA ATCATATCTC ATCCTTGGAC GTGACTCGGA GGCGGCGCCT 
GGTTACTTGG CCATCGGACC GAGCTCGGTG GTCCTTGAAT GGAAGGACGA GTGGTCGCTT 
CGCATGAAGC GGTTCCAGCG GCGGGCACGC AAATGCAGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NetB ( NM_001258739.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245668.1 Drosophila melanogaster Netrin-B (NetB), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa01603
Clone ID Related Accession (Same CDS sequence)	NM_001169284.2 , NM_001258740.2 , NM_078600.4 , NM_001169282.1 , NM_001169283.2 , NM_001258739.2
Accession Version	NM_001258739.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2382bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Netrin-B, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_001258739.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTCAGAG CGACAGGGAC AAGGATGGGC CTGCTCCTGC CCATAATCCT GGCACTGGCC 
ATCGGATCCT CGGCCGCCGG CATATCCTCC AACGATCCCT GCTACTTCGA GGGCAAGCCG 
CGCAAGTGCC TGCCAAGCTT TGTGAATGCC GCCTATGGGA ATCCCGTTCA GGCATCCAGC 
GTTTGTGGCG CCCAACAGCC GGAGCGTTAC TGCGAACTGT TGCGCGACGG AAATGCCGGC 
GAGTGTCGTT CATGCGAACA GCAGCGGTAC GGTCCGGCGG CATTAACCGA TCTAAATAAT 
CCCAGTAATG TGACCTGCTG GCGATCGGGT GCCGTTAATG TGCCCCACGA TCCGGACAGT 
GCGCCGCCGG ATAATGTGAC CCTAACCTTG TCGCTGGGCA AGAAGTACGA GCTGACCTAT 
ATATCGTTGT CATTTTGTCC CCGCTCGCCG CGTCCCGATT CGCTGGCCAT ATTCAAGAGC 
TCGGATTTTG GACAGACCTG GCAACCCTTC CAGTTCTATA GCTCACAGTG TCAAAAGTTC 
TATGGACGAC CGGATCGCGC TAAGATCTCC AAGTTTAATG AGCAGGAGGC CAGATGCATC 
AATTCGCAAC ATGACACGGG CGGAGCTGCT CAGCGATTTG CCTTCAATAC ATTGGAGGGT 
CGTCCGTCGG CCAATGATCT GGATTCCTCG CTGGTCCTGC AGGATTGGGT GACGGCCACG 
GATATAAGAG TTGTATTCCA TCGCCTTGAA TTGCCACCAC AATTGTTGAA GGTGAAAAAT 
GCAAATGCCT TTAGCGATGA GATGGGCGGC AGTCGCGAGG AGGATGAGGA TGATGATGCG 
GATCTGGAAC TGGATGGCGA GCAGGATGAG TACGACTACA ATCTGCAGGA CAACGACAGT 
GCCGATGCTG GGTACGATGA GTACGAGGAG CCCAAGAAGC ATTTGGAACT GGATGATGAT 
CATCTGCATT TGGACTATGC CAGTGATGGT GAATCGGTTG TAAAGAGGCA GGGCAAGCAC 
AAGGGTAGCG CCTATGAGAA GCACTACCAG AGCAAACTGG CGGCCACCAC GCCCCCACAA 
CAGCCGCCCA AGGTGACGCC ACCTGGCAAG GTCACGCCCC CTAGCACAGC TGCCCCTTCT 
GCCGCTGCCT CCGCGGTGAC CTTGCCCATT TCGCAGCATT ACGCCGTATC GGATTTTGCC 
GTCGGCGGAC GATGCAAGTG CAATGGTCAC GCCTCCGAGT GTGTGGCCAC CGTCAGTTCG 
GGATCAGGAA CGGCACTTTC GGATCAGGAC GATGGCCAGG ACGAGGACAC GCCGTCCGCC 
CCCTCGCTGG CCAACCACTT TGGTCGCAGT ACCCAGATGA GCGCCAAACT GACCATGACC 
TGCGCCTGCA AGCACAACAC CGCCGGACCG GAATGCGAAC GCTGCAAACC CTTCTACTTC 
GATCGACCCT GGGGCAGGGC CACCGACAAT GATGCCAACG AATGCAAAAT GTGCCAGTGC 
AACGGACACG CCCGTCGCTG CCGCTTCAAT CTGGAGCTGT ACAAACTATC TGGACGGGTT 
TCGGGAGGTG TATGCTACAA CTGCCAGCAC GATACTACCG GAAGATACTG CCACTATTGC 
CGCGAGGGAT ATTATCGGGA TGCCACTAAG CCGCCAAATC ATCGCAAAGT CTGCAAGCGT 
TGCGATTGCC ATCCAGTGGG CTCGACGGGC AAGACGTGCA ATCACCTGAG TGGCCAGTGT 
CCCTGCAAGG AGGGCGTCAC CGGACTAACT TGCAACCGAT GTGCCCGAGG CTATCAGCAG 
ACCCGCTCCC ACGTGGCACC CTGCATCAAG GTGCCCACTA ATGCGAACAT GATTCAGGCC 
GAGAGTGCTG GCGGCGGTGG AGGAGGTGGC ACTGGTGATT ACAAGGATGG CGGTGGCTCC 
CAGGTCGAGG AGATGAAAAA GTACTGCGGC AAGTGCAAGG CGAGTCCCAA GAAGCTCAAT 
CTCAACAAGT TTTGCATGGA AGATTATGCA ATTTTGGCCA AGGTGATTGG CCACGATCGC 
GCCTCCCAGG ACATCAGCAC GGAAAAGTTC TCGATAGAGC GACAGAACGA GATCTACAAG 
TACGAGATCA ATATCCAGAC GATCTTCAAG CGGAATCCCA TGTCGGGCAC TACGAGTTCC 
TTGCTCGGTA GGGGCAATAT GATGCTGCTG GTGCCGCGCA AGAGCATCGA GTGCCAGTGT 
CCAAAGATAA AGCTAAACAA ATCATATCTC ATCCTTGGAC GTGACTCGGA GGCGGCGCCT 
GGTTACTTGG CCATCGGACC GAGCTCGGTG GTCCTTGAAT GGAAGGACGA GTGGTCGCTT 
CGCATGAAGC GGTTCCAGCG GCGGGCACGC AAATGCAGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245668.1
CDS	387..2768
Translation	MVRATGTRMGLLLPIILALAIGSSAAGISSNDPCYFEGKPRKCLPSFVNAAYGNPVQASSVCGAQQPERYCELLRDGNAGECRSCEQQRYGPAALTDLNNPSNVTCWRSGAVNVPHDPDSAPPDNVTLTLSLGKKYELTYISLSFCPRSPRPDSLAIFKSSDFGQTWQPFQFYSSQCQKFYGRPDRAKISKFNEQEARCINSQHDTGGAAQRFAFNTLEGRPSANDLDSSLVLQDWVTATDIRVVFHRLELPPQLLKVKNANAFSDEMGGSREEDEDDDADLELDGEQDEYDYNLQDNDSADAGYDEYEEPKKHLELDDDHLHLDYASDGESVVKRQGKHKGSAYEKHYQSKLAATTPPQQPPKVTPPGKVTPPSTAAPSAAASAVTLPISQHYAVSDFAVGGRCKCNGHASECVATVSSGSGTALSDQDDGQDEDTPSAPSLANHFGRSTQMSAKLTMTCACKHNTAGPECERCKPFYFDRPWGRATDNDANECKMCQCNGHARRCRFNLELYKLSGRVSGGVCYNCQHDTTGRYCHYCREGYYRDATKPPNHRKVCKRCDCHPVGSTGKTCNHLSGQCPCKEGVTGLTCNRCARGYQQTRSHVAPCIKVPTNANMIQAESAGGGGGGGTGDYKDGGGSQVEEMKKYCGKCKASPKKLNLNKFCMEDYAILAKVIGHDRASQDISTEKFSIERQNEIYKYEINIQTIFKRNPMSGTTSSLLGRGNMMLLVPRKSIECQCPKIKLNKSYLILGRDSEAAPGYLAIGPSSVVLEWKDEWSLRMKRFQRRARKCS

Target ORF information:

RefSeq Version	NM_001258739.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Netrin-B (NetB), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258739.2

ORF Insert Sequence:

ATGGTCAGAG CGACAGGGAC AAGGATGGGC CTGCTCCTGC CCATAATCCT GGCACTGGCC 
ATCGGATCCT CGGCCGCCGG CATATCCTCC AACGATCCCT GCTACTTCGA GGGCAAGCCG 
CGCAAGTGCC TGCCAAGCTT TGTGAATGCC GCCTATGGGA ATCCCGTTCA GGCATCCAGC 
GTTTGTGGCG CCCAACAGCC GGAGCGTTAC TGCGAACTGT TGCGCGACGG AAATGCCGGC 
GAGTGTCGTT CATGCGAACA GCAGCGGTAC GGTCCGGCGG CATTAACCGA TCTAAATAAT 
CCCAGTAATG TGACCTGCTG GCGATCGGGT GCCGTTAATG TGCCCCACGA TCCGGACAGT 
GCGCCGCCGG ATAATGTGAC CCTAACCTTG TCGCTGGGCA AGAAGTACGA GCTGACCTAT 
ATATCGTTGT CATTTTGTCC CCGCTCGCCG CGTCCCGATT CGCTGGCCAT ATTCAAGAGC 
TCGGATTTTG GACAGACCTG GCAACCCTTC CAGTTCTATA GCTCACAGTG TCAAAAGTTC 
TATGGACGAC CGGATCGCGC TAAGATCTCC AAGTTTAATG AGCAGGAGGC CAGATGCATC 
AATTCGCAAC ATGACACGGG CGGAGCTGCT CAGCGATTTG CCTTCAATAC ATTGGAGGGT 
CGTCCGTCGG CCAATGATCT GGATTCCTCG CTGGTCCTGC AGGATTGGGT GACGGCCACG 
GATATAAGAG TTGTATTCCA TCGCCTTGAA TTGCCACCAC AATTGTTGAA GGTGAAAAAT 
GCAAATGCCT TTAGCGATGA GATGGGCGGC AGTCGCGAGG AGGATGAGGA TGATGATGCG 
GATCTGGAAC TGGATGGCGA GCAGGATGAG TACGACTACA ATCTGCAGGA CAACGACAGT 
GCCGATGCTG GGTACGATGA GTACGAGGAG CCCAAGAAGC ATTTGGAACT GGATGATGAT 
CATCTGCATT TGGACTATGC CAGTGATGGT GAATCGGTTG TAAAGAGGCA GGGCAAGCAC 
AAGGGTAGCG CCTATGAGAA GCACTACCAG AGCAAACTGG CGGCCACCAC GCCCCCACAA 
CAGCCGCCCA AGGTGACGCC ACCTGGCAAG GTCACGCCCC CTAGCACAGC TGCCCCTTCT 
GCCGCTGCCT CCGCGGTGAC CTTGCCCATT TCGCAGCATT ACGCCGTATC GGATTTTGCC 
GTCGGCGGAC GATGCAAGTG CAATGGTCAC GCCTCCGAGT GTGTGGCCAC CGTCAGTTCG 
GGATCAGGAA CGGCACTTTC GGATCAGGAC GATGGCCAGG ACGAGGACAC GCCGTCCGCC 
CCCTCGCTGG CCAACCACTT TGGTCGCAGT ACCCAGATGA GCGCCAAACT GACCATGACC 
TGCGCCTGCA AGCACAACAC CGCCGGACCG GAATGCGAAC GCTGCAAACC CTTCTACTTC 
GATCGACCCT GGGGCAGGGC CACCGACAAT GATGCCAACG AATGCAAAAT GTGCCAGTGC 
AACGGACACG CCCGTCGCTG CCGCTTCAAT CTGGAGCTGT ACAAACTATC TGGACGGGTT 
TCGGGAGGTG TATGCTACAA CTGCCAGCAC GATACTACCG GAAGATACTG CCACTATTGC 
CGCGAGGGAT ATTATCGGGA TGCCACTAAG CCGCCAAATC ATCGCAAAGT CTGCAAGCGT 
TGCGATTGCC ATCCAGTGGG CTCGACGGGC AAGACGTGCA ATCACCTGAG TGGCCAGTGT 
CCCTGCAAGG AGGGCGTCAC CGGACTAACT TGCAACCGAT GTGCCCGAGG CTATCAGCAG 
ACCCGCTCCC ACGTGGCACC CTGCATCAAG GTGCCCACTA ATGCGAACAT GATTCAGGCC 
GAGAGTGCTG GCGGCGGTGG AGGAGGTGGC ACTGGTGATT ACAAGGATGG CGGTGGCTCC 
CAGGTCGAGG AGATGAAAAA GTACTGCGGC AAGTGCAAGG CGAGTCCCAA GAAGCTCAAT 
CTCAACAAGT TTTGCATGGA AGATTATGCA ATTTTGGCCA AGGTGATTGG CCACGATCGC 
GCCTCCCAGG ACATCAGCAC GGAAAAGTTC TCGATAGAGC GACAGAACGA GATCTACAAG 
TACGAGATCA ATATCCAGAC GATCTTCAAG CGGAATCCCA TGTCGGGCAC TACGAGTTCC 
TTGCTCGGTA GGGGCAATAT GATGCTGCTG GTGCCGCGCA AGAGCATCGA GTGCCAGTGT 
CCAAAGATAA AGCTAAACAA ATCATATCTC ATCCTTGGAC GTGACTCGGA GGCGGCGCCT 
GGTTACTTGG CCATCGGACC GAGCTCGGTG GTCCTTGAAT GGAAGGACGA GTGGTCGCTT 
CGCATGAAGC GGTTCCAGCG GCGGGCACGC AAATGCAGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NetB ( NM_001272617.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259546.1 Drosophila melanogaster Netrin-B (NetB), transcript variant G, mRNA.

Price & Availability↑

CloneID	OFa03563
Clone ID Related Accession (Same CDS sequence)	NM_001272617.1
Accession Version	NM_001272617.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2358bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Netrin-B, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCCTGC TCCTGCCCAT AATCCTGGCA CTGGCCATCG GATCCTCGGC CGCCGGCATA 
TCCTCCAACG ATCCCTGCTA CTTCGAGGGC AAGCCGCGCA AGTGCCTGCC AAGCTTTGTG 
AATGCCGCCT ATGGGAATCC CGTTCAGGCA TCCAGCGTTT GTGGCGCCCA ACAGCCGGAG 
CGTTACTGCG AACTGTTGCG CGACGGAAAT GCCGGCGAGT GTCGTTCATG CGAACAGCAG 
CGGTACGGTC CGGCGGCATT AACCGATCTA AATAATCCCA GTAATGTGAC CTGCTGGCGA 
TCGGGTGCCG TTAATGTGCC CCACGATCCG GACAGTGCGC CGCCGGATAA TGTGACCCTA 
ACCTTGTCGC TGGGCAAGAA GTACGAGCTG ACCTATATAT CGTTGTCATT TTGTCCCCGC 
TCGCCGCGTC CCGATTCGCT GGCCATATTC AAGAGCTCGG ATTTTGGACA GACCTGGCAA 
CCCTTCCAGT TCTATAGCTC ACAGTGTCAA AAGTTCTATG GACGACCGGA TCGCGCTAAG 
ATCTCCAAGT TTAATGAGCA GGAGGCCAGA TGCATCAATT CGCAACATGA CACGGGCGGA 
GCTGCTCAGC GATTTGCCTT CAATACATTG GAGGGTCGTC CGTCGGCCAA TGATCTGGAT 
TCCTCGCTGG TCCTGCAGGA TTGGGTGACG GCCACGGATA TAAGAGTTGT ATTCCATCGC 
CTTGAATTGC CACCACAATT GTTGAAGGTG AAAAATGCAA ATGCCTTTAG CGATGAGATG 
GGCGGCAGTC GCGAGGAGGA TGAGGATGAT GATGCGGATC TGGAACTGGA TGGCGAGCAG 
GATGAGTACG ACTACAATCT GCAGGACAAC GACAGTGCCG ATGCTGGGTA CGATGAGTAC 
GAGGAGCCCA AGAAGCATTT GGAACTGGAT GATGATCATC TGCATTTGGA CTATGCCAGT 
GATGGTGAAT CGGTTGTAAA GAGGCAGGGC AAGCACAAGG GTAGCGCCTA TGAGAAGCAC 
TACCAGAGCA AACTGGCGGC CACCACGCCC CCACAACAGC CGCCCAAGGT GACGCCACCT 
GGCAAGGTCA CGCCCCCTAG CACAGCTGCC CCTTCTGCCG CTGCCTCCGC GGTGACCTTG 
CCCATTTCGC AGCATTACGC CGTATCGGAT TTTGCCGTCG GCGGACGATG CAAGTGCAAT 
GGTCACGCCT CCGAGTGTGT GGCCACCGTC AGTTCGGGAT CAGGAACGGC ACTTTCGGAT 
CAGGACGATG GCCAGGACGA GGACACGCCG TCCGCCCCCT CGCTGGCCAA CCACTTTGGT 
CGCAGTACCC AGATGAGCGC CAAACTGACC ATGACCTGCG CCTGCAAGCA CAACACCGCC 
GGACCGGAAT GCGAACGCTG CAAACCCTTC TACTTCGATC GACCCTGGGG CAGGGCCACC 
GACAATGATG CCAACGAATG CAAAATGTGC CAGTGCAACG GACACGCCCG TCGCTGCCGC 
TTCAATCTGG AGCTGTACAA ACTATCTGGA CGGGTTTCGG GAGGTGTATG CTACAACTGC 
CAGCACGATA CTACCGGAAG ATACTGCCAC TATTGCCGCG AGGGATATTA TCGGGATGCC 
ACTAAGCCGC CAAATCATCG CAAAGTCTGC AAGCGTTGCG ATTGCCATCC AGTGGGCTCG 
ACGGGCAAGA CGTGCAATCA CCTGAGTGGC CAGTGTCCCT GCAAGGAGGG CGTCACCGGA 
CTAACTTGCA ACCGATGTGC CCGAGGCTAT CAGCAGACCC GCTCCCACGT GGCACCCTGC 
ATCAAGGTGC CCACTAATGC GAACATGATT CAGGCCGAGA GTGCTGGCGG CGGTGGAGGA 
GGTGGCACTG GTGATTACAA GGATGGCGGT GGCTCCCAGG TCGAGGAGAT GAAAAAGTAC 
TGCGGCAAGT GCAAGGCGAG TCCCAAGAAG CTCAATCTCA ACAAGTTTTG CATGGAAGAT 
TATGCAATTT TGGCCAAGGT GATTGGCCAC GATCGCGCCT CCCAGGACAT CAGCACGGAA 
AAGTTCTCGA TAGAGCGACA GAACGAGATC TACAAGTACG AGATCAATAT CCAGACGATC 
TTCAAGCGGA ATCCCATGTC GGGCACTACG AGTTCCTTGC TCGGTAGGGG CAATATGATG 
CTGCTGGTGC CGCGCAAGAG CATCGAGTGC CAGTGTCCAA AGATAAAGCT AAACAAATCA 
TATCTCATCC TTGGACGTGA CTCGGAGGCG GCGCCTGGTT ACTTGGCCAT CGGACCGAGC 
TCGGTGGTCC TTGAATGGAA GGACGAGTGG TCGCTTCGCA TGAAGCGGTT CCAGCGGCGG 
GCACGCAAAT GCAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259546.1
CDS	384..2741
Translation	MGLLLPIILALAIGSSAAGISSNDPCYFEGKPRKCLPSFVNAAYGNPVQASSVCGAQQPERYCELLRDGNAGECRSCEQQRYGPAALTDLNNPSNVTCWRSGAVNVPHDPDSAPPDNVTLTLSLGKKYELTYISLSFCPRSPRPDSLAIFKSSDFGQTWQPFQFYSSQCQKFYGRPDRAKISKFNEQEARCINSQHDTGGAAQRFAFNTLEGRPSANDLDSSLVLQDWVTATDIRVVFHRLELPPQLLKVKNANAFSDEMGGSREEDEDDDADLELDGEQDEYDYNLQDNDSADAGYDEYEEPKKHLELDDDHLHLDYASDGESVVKRQGKHKGSAYEKHYQSKLAATTPPQQPPKVTPPGKVTPPSTAAPSAAASAVTLPISQHYAVSDFAVGGRCKCNGHASECVATVSSGSGTALSDQDDGQDEDTPSAPSLANHFGRSTQMSAKLTMTCACKHNTAGPECERCKPFYFDRPWGRATDNDANECKMCQCNGHARRCRFNLELYKLSGRVSGGVCYNCQHDTTGRYCHYCREGYYRDATKPPNHRKVCKRCDCHPVGSTGKTCNHLSGQCPCKEGVTGLTCNRCARGYQQTRSHVAPCIKVPTNANMIQAESAGGGGGGGTGDYKDGGGSQVEEMKKYCGKCKASPKKLNLNKFCMEDYAILAKVIGHDRASQDISTEKFSIERQNEIYKYEINIQTIFKRNPMSGTTSSLLGRGNMMLLVPRKSIECQCPKIKLNKSYLILGRDSEAAPGYLAIGPSSVVLEWKDEWSLRMKRFQRRARKCS

Target ORF information:

RefSeq Version	NM_001272617.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Netrin-B (NetB), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272617.1

ORF Insert Sequence:

ATGGGCCTGC TCCTGCCCAT AATCCTGGCA CTGGCCATCG GATCCTCGGC CGCCGGCATA 
TCCTCCAACG ATCCCTGCTA CTTCGAGGGC AAGCCGCGCA AGTGCCTGCC AAGCTTTGTG 
AATGCCGCCT ATGGGAATCC CGTTCAGGCA TCCAGCGTTT GTGGCGCCCA ACAGCCGGAG 
CGTTACTGCG AACTGTTGCG CGACGGAAAT GCCGGCGAGT GTCGTTCATG CGAACAGCAG 
CGGTACGGTC CGGCGGCATT AACCGATCTA AATAATCCCA GTAATGTGAC CTGCTGGCGA 
TCGGGTGCCG TTAATGTGCC CCACGATCCG GACAGTGCGC CGCCGGATAA TGTGACCCTA 
ACCTTGTCGC TGGGCAAGAA GTACGAGCTG ACCTATATAT CGTTGTCATT TTGTCCCCGC 
TCGCCGCGTC CCGATTCGCT GGCCATATTC AAGAGCTCGG ATTTTGGACA GACCTGGCAA 
CCCTTCCAGT TCTATAGCTC ACAGTGTCAA AAGTTCTATG GACGACCGGA TCGCGCTAAG 
ATCTCCAAGT TTAATGAGCA GGAGGCCAGA TGCATCAATT CGCAACATGA CACGGGCGGA 
GCTGCTCAGC GATTTGCCTT CAATACATTG GAGGGTCGTC CGTCGGCCAA TGATCTGGAT 
TCCTCGCTGG TCCTGCAGGA TTGGGTGACG GCCACGGATA TAAGAGTTGT ATTCCATCGC 
CTTGAATTGC CACCACAATT GTTGAAGGTG AAAAATGCAA ATGCCTTTAG CGATGAGATG 
GGCGGCAGTC GCGAGGAGGA TGAGGATGAT GATGCGGATC TGGAACTGGA TGGCGAGCAG 
GATGAGTACG ACTACAATCT GCAGGACAAC GACAGTGCCG ATGCTGGGTA CGATGAGTAC 
GAGGAGCCCA AGAAGCATTT GGAACTGGAT GATGATCATC TGCATTTGGA CTATGCCAGT 
GATGGTGAAT CGGTTGTAAA GAGGCAGGGC AAGCACAAGG GTAGCGCCTA TGAGAAGCAC 
TACCAGAGCA AACTGGCGGC CACCACGCCC CCACAACAGC CGCCCAAGGT GACGCCACCT 
GGCAAGGTCA CGCCCCCTAG CACAGCTGCC CCTTCTGCCG CTGCCTCCGC GGTGACCTTG 
CCCATTTCGC AGCATTACGC CGTATCGGAT TTTGCCGTCG GCGGACGATG CAAGTGCAAT 
GGTCACGCCT CCGAGTGTGT GGCCACCGTC AGTTCGGGAT CAGGAACGGC ACTTTCGGAT 
CAGGACGATG GCCAGGACGA GGACACGCCG TCCGCCCCCT CGCTGGCCAA CCACTTTGGT 
CGCAGTACCC AGATGAGCGC CAAACTGACC ATGACCTGCG CCTGCAAGCA CAACACCGCC 
GGACCGGAAT GCGAACGCTG CAAACCCTTC TACTTCGATC GACCCTGGGG CAGGGCCACC 
GACAATGATG CCAACGAATG CAAAATGTGC CAGTGCAACG GACACGCCCG TCGCTGCCGC 
TTCAATCTGG AGCTGTACAA ACTATCTGGA CGGGTTTCGG GAGGTGTATG CTACAACTGC 
CAGCACGATA CTACCGGAAG ATACTGCCAC TATTGCCGCG AGGGATATTA TCGGGATGCC 
ACTAAGCCGC CAAATCATCG CAAAGTCTGC AAGCGTTGCG ATTGCCATCC AGTGGGCTCG 
ACGGGCAAGA CGTGCAATCA CCTGAGTGGC CAGTGTCCCT GCAAGGAGGG CGTCACCGGA 
CTAACTTGCA ACCGATGTGC CCGAGGCTAT CAGCAGACCC GCTCCCACGT GGCACCCTGC 
ATCAAGGTGC CCACTAATGC GAACATGATT CAGGCCGAGA GTGCTGGCGG CGGTGGAGGA 
GGTGGCACTG GTGATTACAA GGATGGCGGT GGCTCCCAGG TCGAGGAGAT GAAAAAGTAC 
TGCGGCAAGT GCAAGGCGAG TCCCAAGAAG CTCAATCTCA ACAAGTTTTG CATGGAAGAT 
TATGCAATTT TGGCCAAGGT GATTGGCCAC GATCGCGCCT CCCAGGACAT CAGCACGGAA 
AAGTTCTCGA TAGAGCGACA GAACGAGATC TACAAGTACG AGATCAATAT CCAGACGATC 
TTCAAGCGGA ATCCCATGTC GGGCACTACG AGTTCCTTGC TCGGTAGGGG CAATATGATG 
CTGCTGGTGC CGCGCAAGAG CATCGAGTGC CAGTGTCCAA AGATAAAGCT AAACAAATCA 
TATCTCATCC TTGGACGTGA CTCGGAGGCG GCGCCTGGTT ACTTGGCCAT CGGACCGAGC 
TCGGTGGTCC TTGAATGGAA GGACGAGTGG TCGCTTCGCA TGAAGCGGTT CCAGCGGCGG 
GCACGCAAAT GCAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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NetB cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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