CG5321 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG5321
Entrez Gene ID	32399
Full Name	CG5321 gene product from transcript CG5321-RB
Synonyms	Dmel\CG5321,anon-EST:Posey66
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 12F2-12F2

mRNA and Protein(s)

mRNA	Protein	Name
NM_132739.3	NP_572967.2	uncharacterized protein, isoform B
NM_001298315.1	NP_001285244.1	uncharacterized protein, isoform C

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	BBOX1	XP_534096.2
Rattus norvegicus (Norway rat)	Bbox1	NP_072151.1
Bos taurus (cattle)	BBOX1	NP_001095351.2
Mus musculus (house mouse)	Bbox1	NP_569719.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008246	XP_317222.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012477	XP_307672.4
Homo sapiens (human)	BBOX1	NP_003977.1
Neurospora crassa	NCU06891	XP_959238.1
Xenopus tropicalis (tropical clawed frog)	bbox1	NP_001120574.1
Pan troglodytes (chimpanzee)	BBOX1	XP_001135704.1
Gallus gallus (chicken)	BBOX1	XP_424543.2
Danio rerio (zebrafish)	bbox1	NP_001017717.1
Drosophila melanogaster (fruit fly)	CG5321	NP_572967.2
Drosophila melanogaster (fruit fly)	CG10814	NP_610868.1
Caenorhabditis elegans (roundworm)	gbh-1	NP_496305.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG5321 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG5321 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa01602	NM_132739.3 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG5321), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa01602	NM_001298315.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant C (CG5321), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG5321 ( NM_132739.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572967.2 Drosophila melanogaster uncharacterized protein, transcript variant B (CG5321), mRNA.

Price & Availability↑

CloneID	OFa01602
Clone ID Related Accession (Same CDS sequence)	NM_132739.3 , NM_001298315.1
Accession Version	NM_132739.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1242bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_132739.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGGGTTC CGAGATTACT GGGTCGTTCC CTGAGTCAAC TGAAGACCAT TAACTCCGCT 
CAGTTTTCTC GCCAAATCAG TGCCAAGATC GTGAATGAAT CGAGTATCGA GGTTCAACCG 
GAGGCAAATT CCAAACCGCT TACTTTTCCG GGTATTTGGC TGCGGGACAA TTGCATGTGC 
GAGGATTGCT TCCACGGCAG CTCCAAGTCG CGTAAGCTGG ACTGGGATAA CTTCGATACC 
CGCATCCGGC CAGTCAGCCT GCAGACAGAT GAGCAATCCA AGGAAGTGCT GGTCAAGTGG 
AGCGATGCAC ACGAGAGCAG GTTCTCTTTG AAGTGGCTAA AGGAGCGATG TTTCGAGCCT 
GATAAACAGC AGGAATACCT GCGGGACTTC TACAGACCCA CGACTAGACA CTGGTCGGGT 
GCAGAGTTTC AGGATATTGC CCAGCACTTT AGCTACGATG AGGTGATGTC CCAGGATTCG 
GTGCTGATGC AGTGGCTCCA GGCGCTGGCC ATCTACGGTG TGGCTCTGCT CCGAGGAGCT 
CCACTGGACG AGGGCGTGGT GCGACGACTG GCGGACCGGG TGGGCTTCAT CCGGCGCACC 
ACCTACGGCG AGGAGTTTGT GGTGCAGGCC AAGCCGGGTG CCCAGAACTT CGCCTACCTT 
TCGCTGACAC TGCCCCTCCA CACGGATCTG CCCTACTACG AGTACAAGCC AAGCGTCAAC 
ATCCTGCACT GCGTTGTCCA AACGGATTCA CCTGGTGGCA GCAATATGCT CGTGGATGGC 
TTCCATGTCG CCGATCTCCT GCGTCGCGAT CATCCCGAAG ACTTCGAGCG ACTGAGTCGC 
ATAGTAGTCG ATTGGAACGA CATTGGCAGC GAGGATGGAC GAGAGTTCCA CAACATTTGG 
AGGGCACCAG TCATTTGCTT GGACGAGGAG GGGCGATACA CGCGGATAAA TCACAGCGTG 
CCGCAGCGGG ACAGTCACTT CAATGTGCCG CTCGAGGAGG TCCTTCCGTG GTATGAGTCC 
TACGCCCTTT TCGTTCGCCT GGCCATCGCC GATAGCCACG CCTTCAAAAC GCGTCCCGGC 
GACGTCCTGA CCTTCAACAA TATTCGCCTG CTCCACGGAC GTACTGGTTA TGATGATTCG 
GAGGAGAGTC CGCGCTATAT TGTGGGCGCC TATCTGGACT GGGACATCAT ATATTCGCGC 
CTAAGGGTAT TGAAAAAACG CTTGACTGCG AGTTGCCAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572967.2
CDS	37..1278
Translation	MWVPRLLGRSLSQLKTINSAQFSRQISAKIVNESSIEVQPEANSKPLTFPGIWLRDNCMCEDCFHGSSKSRKLDWDNFDTRIRPVSLQTDEQSKEVLVKWSDAHESRFSLKWLKERCFEPDKQQEYLRDFYRPTTRHWSGAEFQDIAQHFSYDEVMSQDSVLMQWLQALAIYGVALLRGAPLDEGVVRRLADRVGFIRRTTYGEEFVVQAKPGAQNFAYLSLTLPLHTDLPYYEYKPSVNILHCVVQTDSPGGSNMLVDGFHVADLLRRDHPEDFERLSRIVVDWNDIGSEDGREFHNIWRAPVICLDEEGRYTRINHSVPQRDSHFNVPLEEVLPWYESYALFVRLAIADSHAFKTRPGDVLTFNNIRLLHGRTGYDDSEESPRYIVGAYLDWDIIYSRLRVLKKRLTASCQ

Target ORF information:

RefSeq Version	NM_132739.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG5321), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132739.3

ORF Insert Sequence:

ATGTGGGTTC CGAGATTACT GGGTCGTTCC CTGAGTCAAC TGAAGACCAT TAACTCCGCT 
CAGTTTTCTC GCCAAATCAG TGCCAAGATC GTGAATGAAT CGAGTATCGA GGTTCAACCG 
GAGGCAAATT CCAAACCGCT TACTTTTCCG GGTATTTGGC TGCGGGACAA TTGCATGTGC 
GAGGATTGCT TCCACGGCAG CTCCAAGTCG CGTAAGCTGG ACTGGGATAA CTTCGATACC 
CGCATCCGGC CAGTCAGCCT GCAGACAGAT GAGCAATCCA AGGAAGTGCT GGTCAAGTGG 
AGCGATGCAC ACGAGAGCAG GTTCTCTTTG AAGTGGCTAA AGGAGCGATG TTTCGAGCCT 
GATAAACAGC AGGAATACCT GCGGGACTTC TACAGACCCA CGACTAGACA CTGGTCGGGT 
GCAGAGTTTC AGGATATTGC CCAGCACTTT AGCTACGATG AGGTGATGTC CCAGGATTCG 
GTGCTGATGC AGTGGCTCCA GGCGCTGGCC ATCTACGGTG TGGCTCTGCT CCGAGGAGCT 
CCACTGGACG AGGGCGTGGT GCGACGACTG GCGGACCGGG TGGGCTTCAT CCGGCGCACC 
ACCTACGGCG AGGAGTTTGT GGTGCAGGCC AAGCCGGGTG CCCAGAACTT CGCCTACCTT 
TCGCTGACAC TGCCCCTCCA CACGGATCTG CCCTACTACG AGTACAAGCC AAGCGTCAAC 
ATCCTGCACT GCGTTGTCCA AACGGATTCA CCTGGTGGCA GCAATATGCT CGTGGATGGC 
TTCCATGTCG CCGATCTCCT GCGTCGCGAT CATCCCGAAG ACTTCGAGCG ACTGAGTCGC 
ATAGTAGTCG ATTGGAACGA CATTGGCAGC GAGGATGGAC GAGAGTTCCA CAACATTTGG 
AGGGCACCAG TCATTTGCTT GGACGAGGAG GGGCGATACA CGCGGATAAA TCACAGCGTG 
CCGCAGCGGG ACAGTCACTT CAATGTGCCG CTCGAGGAGG TCCTTCCGTG GTATGAGTCC 
TACGCCCTTT TCGTTCGCCT GGCCATCGCC GATAGCCACG CCTTCAAAAC GCGTCCCGGC 
GACGTCCTGA CCTTCAACAA TATTCGCCTG CTCCACGGAC GTACTGGTTA TGATGATTCG 
GAGGAGAGTC CGCGCTATAT TGTGGGCGCC TATCTGGACT GGGACATCAT ATATTCGCGC 
CTAAGGGTAT TGAAAAAACG CTTGACTGCG AGTTGCCAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG5321 ( NM_001298315.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285244.1 Drosophila melanogaster uncharacterized protein, transcript variant C (CG5321), mRNA.

Price & Availability↑

CloneID	OFa01602
Clone ID Related Accession (Same CDS sequence)	NM_132739.3 , NM_001298315.1
Accession Version	NM_001298315.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1242bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGGGTTC CGAGATTACT GGGTCGTTCC CTGAGTCAAC TGAAGACCAT TAACTCCGCT 
CAGTTTTCTC GCCAAATCAG TGCCAAGATC GTGAATGAAT CGAGTATCGA GGTTCAACCG 
GAGGCAAATT CCAAACCGCT TACTTTTCCG GGTATTTGGC TGCGGGACAA TTGCATGTGC 
GAGGATTGCT TCCACGGCAG CTCCAAGTCG CGTAAGCTGG ACTGGGATAA CTTCGATACC 
CGCATCCGGC CAGTCAGCCT GCAGACAGAT GAGCAATCCA AGGAAGTGCT GGTCAAGTGG 
AGCGATGCAC ACGAGAGCAG GTTCTCTTTG AAGTGGCTAA AGGAGCGATG TTTCGAGCCT 
GATAAACAGC AGGAATACCT GCGGGACTTC TACAGACCCA CGACTAGACA CTGGTCGGGT 
GCAGAGTTTC AGGATATTGC CCAGCACTTT AGCTACGATG AGGTGATGTC CCAGGATTCG 
GTGCTGATGC AGTGGCTCCA GGCGCTGGCC ATCTACGGTG TGGCTCTGCT CCGAGGAGCT 
CCACTGGACG AGGGCGTGGT GCGACGACTG GCGGACCGGG TGGGCTTCAT CCGGCGCACC 
ACCTACGGCG AGGAGTTTGT GGTGCAGGCC AAGCCGGGTG CCCAGAACTT CGCCTACCTT 
TCGCTGACAC TGCCCCTCCA CACGGATCTG CCCTACTACG AGTACAAGCC AAGCGTCAAC 
ATCCTGCACT GCGTTGTCCA AACGGATTCA CCTGGTGGCA GCAATATGCT CGTGGATGGC 
TTCCATGTCG CCGATCTCCT GCGTCGCGAT CATCCCGAAG ACTTCGAGCG ACTGAGTCGC 
ATAGTAGTCG ATTGGAACGA CATTGGCAGC GAGGATGGAC GAGAGTTCCA CAACATTTGG 
AGGGCACCAG TCATTTGCTT GGACGAGGAG GGGCGATACA CGCGGATAAA TCACAGCGTG 
CCGCAGCGGG ACAGTCACTT CAATGTGCCG CTCGAGGAGG TCCTTCCGTG GTATGAGTCC 
TACGCCCTTT TCGTTCGCCT GGCCATCGCC GATAGCCACG CCTTCAAAAC GCGTCCCGGC 
GACGTCCTGA CCTTCAACAA TATTCGCCTG CTCCACGGAC GTACTGGTTA TGATGATTCG 
GAGGAGAGTC CGCGCTATAT TGTGGGCGCC TATCTGGACT GGGACATCAT ATATTCGCGC 
CTAAGGGTAT TGAAAAAACG CTTGACTGCG AGTTGCCAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285244.1
CDS	37..1278
Translation	MWVPRLLGRSLSQLKTINSAQFSRQISAKIVNESSIEVQPEANSKPLTFPGIWLRDNCMCEDCFHGSSKSRKLDWDNFDTRIRPVSLQTDEQSKEVLVKWSDAHESRFSLKWLKERCFEPDKQQEYLRDFYRPTTRHWSGAEFQDIAQHFSYDEVMSQDSVLMQWLQALAIYGVALLRGAPLDEGVVRRLADRVGFIRRTTYGEEFVVQAKPGAQNFAYLSLTLPLHTDLPYYEYKPSVNILHCVVQTDSPGGSNMLVDGFHVADLLRRDHPEDFERLSRIVVDWNDIGSEDGREFHNIWRAPVICLDEEGRYTRINHSVPQRDSHFNVPLEEVLPWYESYALFVRLAIADSHAFKTRPGDVLTFNNIRLLHGRTGYDDSEESPRYIVGAYLDWDIIYSRLRVLKKRLTASCQ

Target ORF information:

RefSeq Version	NM_001298315.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant C (CG5321), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298315.1

ORF Insert Sequence:

ATGTGGGTTC CGAGATTACT GGGTCGTTCC CTGAGTCAAC TGAAGACCAT TAACTCCGCT 
CAGTTTTCTC GCCAAATCAG TGCCAAGATC GTGAATGAAT CGAGTATCGA GGTTCAACCG 
GAGGCAAATT CCAAACCGCT TACTTTTCCG GGTATTTGGC TGCGGGACAA TTGCATGTGC 
GAGGATTGCT TCCACGGCAG CTCCAAGTCG CGTAAGCTGG ACTGGGATAA CTTCGATACC 
CGCATCCGGC CAGTCAGCCT GCAGACAGAT GAGCAATCCA AGGAAGTGCT GGTCAAGTGG 
AGCGATGCAC ACGAGAGCAG GTTCTCTTTG AAGTGGCTAA AGGAGCGATG TTTCGAGCCT 
GATAAACAGC AGGAATACCT GCGGGACTTC TACAGACCCA CGACTAGACA CTGGTCGGGT 
GCAGAGTTTC AGGATATTGC CCAGCACTTT AGCTACGATG AGGTGATGTC CCAGGATTCG 
GTGCTGATGC AGTGGCTCCA GGCGCTGGCC ATCTACGGTG TGGCTCTGCT CCGAGGAGCT 
CCACTGGACG AGGGCGTGGT GCGACGACTG GCGGACCGGG TGGGCTTCAT CCGGCGCACC 
ACCTACGGCG AGGAGTTTGT GGTGCAGGCC AAGCCGGGTG CCCAGAACTT CGCCTACCTT 
TCGCTGACAC TGCCCCTCCA CACGGATCTG CCCTACTACG AGTACAAGCC AAGCGTCAAC 
ATCCTGCACT GCGTTGTCCA AACGGATTCA CCTGGTGGCA GCAATATGCT CGTGGATGGC 
TTCCATGTCG CCGATCTCCT GCGTCGCGAT CATCCCGAAG ACTTCGAGCG ACTGAGTCGC 
ATAGTAGTCG ATTGGAACGA CATTGGCAGC GAGGATGGAC GAGAGTTCCA CAACATTTGG 
AGGGCACCAG TCATTTGCTT GGACGAGGAG GGGCGATACA CGCGGATAAA TCACAGCGTG 
CCGCAGCGGG ACAGTCACTT CAATGTGCCG CTCGAGGAGG TCCTTCCGTG GTATGAGTCC 
TACGCCCTTT TCGTTCGCCT GGCCATCGCC GATAGCCACG CCTTCAAAAC GCGTCCCGGC 
GACGTCCTGA CCTTCAACAA TATTCGCCTG CTCCACGGAC GTACTGGTTA TGATGATTCG 
GAGGAGAGTC CGCGCTATAT TGTGGGCGCC TATCTGGACT GGGACATCAT ATATTCGCGC 
CTAAGGGTAT TGAAAAAACG CTTGACTGCG AGTTGCCAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG5321 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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