NetA cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	NetA
Entrez Gene ID	32398
Full Name	Netrin-A
Synonyms	CG18657,CT27014,Dmel\CG18657,NetrinA,net,netA,netrin,netrin A
General protein information	Preferred Names Netrin-A Names CG18657 gene product from transcript CG18657-RC CG18657-PA CG18657-PB CG18657-PC D-netrin D-netrin-A NetA-PA NetA-PB NetA-PC NetrinA netrin netrin A
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 12F1-12F2

mRNA and Protein(s)

mRNA	Protein	Name
NM_078599.5	NP_511154.3	Netrin-A, isoform A
NM_001258738.2	NP_001245667.1	Netrin-A, isoform B
NM_001298314.1	NP_001285243.1	Netrin-A, isoform C

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	NetA	NP_511154.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000225	XP_310908.5
Danio rerio (zebrafish)	ntn1b	NP_571073.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

mutation of mushroom body defect (mud) dramatically enhances the phenotype of Netrin or frazzled mutants, resulting in many more axons failing to cross the midline, although mutations in mud alone have little effect.
Title: Mushroom body defect is required in parallel to Netrin for midline axon guidance in Drosophila.

Contact-mediated cell adhesion via Fmi acts in addition to the Net/Fra guidance system during axon pathfinding across the midline, underlining the importance of PCP molecules during vertebrates and invertebrates midline development.
Title: Flamingo, a seven-pass transmembrane cadherin, cooperates with Netrin/Frazzled in Drosophila midline guidance.

These data are the first to show that Netrin and Frazzled regulate the placement of gap junctions presynaptically at a synapse.
Title: Netrin and Frazzled regulate presynaptic gap junctions at a Drosophila giant synapse.

adult Drosophila lacking both netrin genes, NetA and NetB, have fertility defects in both sexes together with an inability to fly and reduced viability.
Title: Control of male and female fertility by the netrin axon guidance genes.

In peripodial cells Frazzled is endocytosed and undergoes NetA-dependent degradation, which is required for eversion.
Title: Netrin-dependent downregulation of Frazzled/DCC is required for the dissociation of the peripodial epithelium in Drosophila.

The following NetA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NetA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01601	NM_001298314.1 Latest version!	Drosophila melanogaster Netrin-A (NetA), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa01601	NM_078599.5 Latest version!	Drosophila melanogaster Netrin-A (NetA), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa03562	NM_001258738.2 Latest version!	Drosophila melanogaster Netrin-A (NetA), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NetA ( NM_078599.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_511154.3 Drosophila melanogaster Netrin-A (NetA), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa01601
Clone ID Related Accession (Same CDS sequence)	NM_078599.5 , NM_001298314.1
Accession Version	NM_078599.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2181bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Netrin-A, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_078599.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATCCGTG GAATCTTGCT CCTGCTGCTG GGAACCACCA GGTTCAGCCC CATCCAGTGC 
ATCTCCAATG ACGTGTACTT CAAGATGTTT TCGCAACAGG CGCCGCCGGA GGATCCGTGC 
TACAATAAAG CGCACGAGCC ACGCGCCTGC ATTCCGGACT TTGTGAACGC CGCCTACGAT 
GCTCCAGTGG TGGCCAGTTC CACGTGCGGA TCGTCGGGTG CGCAGCGATA TTGCGAGTAC 
CAGGATCACG AGCGCTCCTG CCACACCTGC GACATGACGG ATCCGTTGCG CAGCTTTCCA 
GCCCGCTCGC TGACCGATTT GAATAACTCG AATAATGTGA CCTGCTGGCG CAGTGAGCCG 
GTGACCGGAA GTGGGGACAA CGTAACCTTG ACCCTCTCGC TGGGCAAGAA GTTCGAGCTG 
ACCTATGTGA TCCTACAGCT GTGCCCGCAT GCTCCGCGTC CCGATTCGAT GGTGATCTAC 
AAGAGCACCG ATCACGGGCT CAGTTGGCAG CCATTCCAGT TCTTTAGCTC CCAGTGCCGC 
CGGCTTTTCG GACGTCCCGC CCGCCAATCC ACTGGACGCC ACAATGAGCA CGAGGCCCGC 
TGCAGCGATG TCACCAGGCC ACTGGTCTCC AGAATTGCCT TCAGCACACT GGAGGGACGC 
CCATCGTCAC GGGATCTGGA CTCATCGCCG GTGCTGCAGG ACTGGGTGAC GGCCACCGAT 
ATCCGGGTGG TGTTCCATCG CCTTCAACGC CCCGATCCGC AGGCCCTGCT CTCATTGGAA 
GCCGGAGGAG CAACGGATTT GGCCAGCGGC AAGTACAGTG TGCCCCTGGC CAATGGACCC 
GCTGGCAATA ATATTGAGGC GAATCTCGGT GGGGATGTGG CAACATCCGG CAGTGGATTG 
CACTATGCCA TCTCTGACTT TTCGGTGGGC GGACGGTGCA AGTGCAATGG ACACGCCTCC 
AAGTGTTCCA CGGACGCCAG TGGGCAGCTG AACTGCGAGT GCAGCCACAA TACCGCTGGA 
AGGGATTGCG AACGCTGCAA GCCATTCCAC TTCGATCGTC CATGGGCCAG AGCCACCGCC 
AAGGAAGCCA ATGAGTGCAA AGAGTGCAAC TGCAACAAGC ACGCCCGTCA GTGCCGCTTC 
AATATGGAAA TCTTTCGGCT TTCGCAGGGC GTCTCCGGCG GAGTCTGCCA GAATTGTCGC 
CACTCGACTA CGGGCCGGAA TTGTCACCAG TGCAAGGAGG GATTCTATCG GGACGCCACC 
AAACCACTCA CCCATCGCAA GGTTTGCAAA GCCTGCGATT GCCATCCGAT CGGGTCATCT 
GGCAAGATCT GCAACAGCAC CAGCGGTCAA TGTCCCTGCA AGGACGGGGT CACCGGACTG 
ACCTGCAACC GCTGCGCTCG AGGTTACCAG CAAAGTCGCT CCCACATCGC CCCCTGCATA 
AAACAACCAC CGCGTATGAT CAACATGCTG GACACCCAGA ATACCGCTCC CGAGCCGGAT 
GAGCCCGAAT CCTCTCCAGG ATCTGGTGGC GACCGCAACG GCGCCGCCGG AATGGCCGCC 
CAGTCTCAGT ACTATCGCAC CGAGGGCGGC AGGGAATGCG GCAAATGCAG AGTCAGCACC 
AAAAGGCTAA ACCTAAACAA GTTCTGCAAA AGAGACTACG CAATAATGGC CAAAGTAATT 
GGTCGCGATA CGAGCAGCGA GGCGGTCAGC AGGGAAGTTC AACGGCGCGC AATGGATCCC 
GACGTGGCGG ACTACGAAAT GGATCAGGTG CAGCCGGGAT CGGCCCGATC TCCGATCACG 
GGGGTGTACG AGTTTCAGGC GGCGGACTAT CCCAATCCCA ATCCCAATCC CAGAGGCAGC 
GAAATGGAGC GCTTCGATCT GCAGATCCAG GCCGTCTTCA AGCGCAGCAG GCCAGGCGAA 
AGCAGCGGCG CGGGGAACGT GTACGGAATG CCGAATACAA CCCTAAAACG CGGTCCTATG 
ACCTGGATCA TACCGACTAA GGACCTGGAG TGCCGCTGCC CCAGGATCAG AGTAAACAGA 
TCCTATTTGA TCTTGGGTCG GGACTCGGAG GCGCCACCCG GTTACCTGGG CATCGGACCG 
CACTCGATCG TAATCGAATG GAAGGAGGAC TGGTATCGAC GCATGAAGCG CTTCCAACGG 
AGAGCGCGTA CTTGCGCGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_511154.3
CDS	591..2771
Translation	MIRGILLLLLGTTRFSPIQCISNDVYFKMFSQQAPPEDPCYNKAHEPRACIPDFVNAAYDAPVVASSTCGSSGAQRYCEYQDHERSCHTCDMTDPLRSFPARSLTDLNNSNNVTCWRSEPVTGSGDNVTLTLSLGKKFELTYVILQLCPHAPRPDSMVIYKSTDHGLSWQPFQFFSSQCRRLFGRPARQSTGRHNEHEARCSDVTRPLVSRIAFSTLEGRPSSRDLDSSPVLQDWVTATDIRVVFHRLQRPDPQALLSLEAGGATDLASGKYSVPLANGPAGNNIEANLGGDVATSGSGLHYAISDFSVGGRCKCNGHASKCSTDASGQLNCECSHNTAGRDCERCKPFHFDRPWARATAKEANECKECNCNKHARQCRFNMEIFRLSQGVSGGVCQNCRHSTTGRNCHQCKEGFYRDATKPLTHRKVCKACDCHPIGSSGKICNSTSGQCPCKDGVTGLTCNRCARGYQQSRSHIAPCIKQPPRMINMLDTQNTAPEPDEPESSPGSGGDRNGAAGMAAQSQYYRTEGGRECGKCRVSTKRLNLNKFCKRDYAIMAKVIGRDTSSEAVSREVQRRAMDPDVADYEMDQVQPGSARSPITGVYEFQAADYPNPNPNPRGSEMERFDLQIQAVFKRSRPGESSGAGNVYGMPNTTLKRGPMTWIIPTKDLECRCPRIRVNRSYLILGRDSEAPPGYLGIGPHSIVIEWKEDWYRRMKRFQRRARTCA

Target ORF information:

RefSeq Version	NM_078599.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Netrin-A (NetA), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078599.5

ORF Insert Sequence:

ATGATCCGTG GAATCTTGCT CCTGCTGCTG GGAACCACCA GGTTCAGCCC CATCCAGTGC 
ATCTCCAATG ACGTGTACTT CAAGATGTTT TCGCAACAGG CGCCGCCGGA GGATCCGTGC 
TACAATAAAG CGCACGAGCC ACGCGCCTGC ATTCCGGACT TTGTGAACGC CGCCTACGAT 
GCTCCAGTGG TGGCCAGTTC CACGTGCGGA TCGTCGGGTG CGCAGCGATA TTGCGAGTAC 
CAGGATCACG AGCGCTCCTG CCACACCTGC GACATGACGG ATCCGTTGCG CAGCTTTCCA 
GCCCGCTCGC TGACCGATTT GAATAACTCG AATAATGTGA CCTGCTGGCG CAGTGAGCCG 
GTGACCGGAA GTGGGGACAA CGTAACCTTG ACCCTCTCGC TGGGCAAGAA GTTCGAGCTG 
ACCTATGTGA TCCTACAGCT GTGCCCGCAT GCTCCGCGTC CCGATTCGAT GGTGATCTAC 
AAGAGCACCG ATCACGGGCT CAGTTGGCAG CCATTCCAGT TCTTTAGCTC CCAGTGCCGC 
CGGCTTTTCG GACGTCCCGC CCGCCAATCC ACTGGACGCC ACAATGAGCA CGAGGCCCGC 
TGCAGCGATG TCACCAGGCC ACTGGTCTCC AGAATTGCCT TCAGCACACT GGAGGGACGC 
CCATCGTCAC GGGATCTGGA CTCATCGCCG GTGCTGCAGG ACTGGGTGAC GGCCACCGAT 
ATCCGGGTGG TGTTCCATCG CCTTCAACGC CCCGATCCGC AGGCCCTGCT CTCATTGGAA 
GCCGGAGGAG CAACGGATTT GGCCAGCGGC AAGTACAGTG TGCCCCTGGC CAATGGACCC 
GCTGGCAATA ATATTGAGGC GAATCTCGGT GGGGATGTGG CAACATCCGG CAGTGGATTG 
CACTATGCCA TCTCTGACTT TTCGGTGGGC GGACGGTGCA AGTGCAATGG ACACGCCTCC 
AAGTGTTCCA CGGACGCCAG TGGGCAGCTG AACTGCGAGT GCAGCCACAA TACCGCTGGA 
AGGGATTGCG AACGCTGCAA GCCATTCCAC TTCGATCGTC CATGGGCCAG AGCCACCGCC 
AAGGAAGCCA ATGAGTGCAA AGAGTGCAAC TGCAACAAGC ACGCCCGTCA GTGCCGCTTC 
AATATGGAAA TCTTTCGGCT TTCGCAGGGC GTCTCCGGCG GAGTCTGCCA GAATTGTCGC 
CACTCGACTA CGGGCCGGAA TTGTCACCAG TGCAAGGAGG GATTCTATCG GGACGCCACC 
AAACCACTCA CCCATCGCAA GGTTTGCAAA GCCTGCGATT GCCATCCGAT CGGGTCATCT 
GGCAAGATCT GCAACAGCAC CAGCGGTCAA TGTCCCTGCA AGGACGGGGT CACCGGACTG 
ACCTGCAACC GCTGCGCTCG AGGTTACCAG CAAAGTCGCT CCCACATCGC CCCCTGCATA 
AAACAACCAC CGCGTATGAT CAACATGCTG GACACCCAGA ATACCGCTCC CGAGCCGGAT 
GAGCCCGAAT CCTCTCCAGG ATCTGGTGGC GACCGCAACG GCGCCGCCGG AATGGCCGCC 
CAGTCTCAGT ACTATCGCAC CGAGGGCGGC AGGGAATGCG GCAAATGCAG AGTCAGCACC 
AAAAGGCTAA ACCTAAACAA GTTCTGCAAA AGAGACTACG CAATAATGGC CAAAGTAATT 
GGTCGCGATA CGAGCAGCGA GGCGGTCAGC AGGGAAGTTC AACGGCGCGC AATGGATCCC 
GACGTGGCGG ACTACGAAAT GGATCAGGTG CAGCCGGGAT CGGCCCGATC TCCGATCACG 
GGGGTGTACG AGTTTCAGGC GGCGGACTAT CCCAATCCCA ATCCCAATCC CAGAGGCAGC 
GAAATGGAGC GCTTCGATCT GCAGATCCAG GCCGTCTTCA AGCGCAGCAG GCCAGGCGAA 
AGCAGCGGCG CGGGGAACGT GTACGGAATG CCGAATACAA CCCTAAAACG CGGTCCTATG 
ACCTGGATCA TACCGACTAA GGACCTGGAG TGCCGCTGCC CCAGGATCAG AGTAAACAGA 
TCCTATTTGA TCTTGGGTCG GGACTCGGAG GCGCCACCCG GTTACCTGGG CATCGGACCG 
CACTCGATCG TAATCGAATG GAAGGAGGAC TGGTATCGAC GCATGAAGCG CTTCCAACGG 
AGAGCGCGTA CTTGCGCGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NetA ( NM_001298314.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285243.1 Drosophila melanogaster Netrin-A (NetA), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa01601
Clone ID Related Accession (Same CDS sequence)	NM_078599.5 , NM_001298314.1
Accession Version	NM_001298314.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2181bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Netrin-A, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATCCGTG GAATCTTGCT CCTGCTGCTG GGAACCACCA GGTTCAGCCC CATCCAGTGC 
ATCTCCAATG ACGTGTACTT CAAGATGTTT TCGCAACAGG CGCCGCCGGA GGATCCGTGC 
TACAATAAAG CGCACGAGCC ACGCGCCTGC ATTCCGGACT TTGTGAACGC CGCCTACGAT 
GCTCCAGTGG TGGCCAGTTC CACGTGCGGA TCGTCGGGTG CGCAGCGATA TTGCGAGTAC 
CAGGATCACG AGCGCTCCTG CCACACCTGC GACATGACGG ATCCGTTGCG CAGCTTTCCA 
GCCCGCTCGC TGACCGATTT GAATAACTCG AATAATGTGA CCTGCTGGCG CAGTGAGCCG 
GTGACCGGAA GTGGGGACAA CGTAACCTTG ACCCTCTCGC TGGGCAAGAA GTTCGAGCTG 
ACCTATGTGA TCCTACAGCT GTGCCCGCAT GCTCCGCGTC CCGATTCGAT GGTGATCTAC 
AAGAGCACCG ATCACGGGCT CAGTTGGCAG CCATTCCAGT TCTTTAGCTC CCAGTGCCGC 
CGGCTTTTCG GACGTCCCGC CCGCCAATCC ACTGGACGCC ACAATGAGCA CGAGGCCCGC 
TGCAGCGATG TCACCAGGCC ACTGGTCTCC AGAATTGCCT TCAGCACACT GGAGGGACGC 
CCATCGTCAC GGGATCTGGA CTCATCGCCG GTGCTGCAGG ACTGGGTGAC GGCCACCGAT 
ATCCGGGTGG TGTTCCATCG CCTTCAACGC CCCGATCCGC AGGCCCTGCT CTCATTGGAA 
GCCGGAGGAG CAACGGATTT GGCCAGCGGC AAGTACAGTG TGCCCCTGGC CAATGGACCC 
GCTGGCAATA ATATTGAGGC GAATCTCGGT GGGGATGTGG CAACATCCGG CAGTGGATTG 
CACTATGCCA TCTCTGACTT TTCGGTGGGC GGACGGTGCA AGTGCAATGG ACACGCCTCC 
AAGTGTTCCA CGGACGCCAG TGGGCAGCTG AACTGCGAGT GCAGCCACAA TACCGCTGGA 
AGGGATTGCG AACGCTGCAA GCCATTCCAC TTCGATCGTC CATGGGCCAG AGCCACCGCC 
AAGGAAGCCA ATGAGTGCAA AGAGTGCAAC TGCAACAAGC ACGCCCGTCA GTGCCGCTTC 
AATATGGAAA TCTTTCGGCT TTCGCAGGGC GTCTCCGGCG GAGTCTGCCA GAATTGTCGC 
CACTCGACTA CGGGCCGGAA TTGTCACCAG TGCAAGGAGG GATTCTATCG GGACGCCACC 
AAACCACTCA CCCATCGCAA GGTTTGCAAA GCCTGCGATT GCCATCCGAT CGGGTCATCT 
GGCAAGATCT GCAACAGCAC CAGCGGTCAA TGTCCCTGCA AGGACGGGGT CACCGGACTG 
ACCTGCAACC GCTGCGCTCG AGGTTACCAG CAAAGTCGCT CCCACATCGC CCCCTGCATA 
AAACAACCAC CGCGTATGAT CAACATGCTG GACACCCAGA ATACCGCTCC CGAGCCGGAT 
GAGCCCGAAT CCTCTCCAGG ATCTGGTGGC GACCGCAACG GCGCCGCCGG AATGGCCGCC 
CAGTCTCAGT ACTATCGCAC CGAGGGCGGC AGGGAATGCG GCAAATGCAG AGTCAGCACC 
AAAAGGCTAA ACCTAAACAA GTTCTGCAAA AGAGACTACG CAATAATGGC CAAAGTAATT 
GGTCGCGATA CGAGCAGCGA GGCGGTCAGC AGGGAAGTTC AACGGCGCGC AATGGATCCC 
GACGTGGCGG ACTACGAAAT GGATCAGGTG CAGCCGGGAT CGGCCCGATC TCCGATCACG 
GGGGTGTACG AGTTTCAGGC GGCGGACTAT CCCAATCCCA ATCCCAATCC CAGAGGCAGC 
GAAATGGAGC GCTTCGATCT GCAGATCCAG GCCGTCTTCA AGCGCAGCAG GCCAGGCGAA 
AGCAGCGGCG CGGGGAACGT GTACGGAATG CCGAATACAA CCCTAAAACG CGGTCCTATG 
ACCTGGATCA TACCGACTAA GGACCTGGAG TGCCGCTGCC CCAGGATCAG AGTAAACAGA 
TCCTATTTGA TCTTGGGTCG GGACTCGGAG GCGCCACCCG GTTACCTGGG CATCGGACCG 
CACTCGATCG TAATCGAATG GAAGGAGGAC TGGTATCGAC GCATGAAGCG CTTCCAACGG 
AGAGCGCGTA CTTGCGCGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285243.1
CDS	685..2865
Translation	MIRGILLLLLGTTRFSPIQCISNDVYFKMFSQQAPPEDPCYNKAHEPRACIPDFVNAAYDAPVVASSTCGSSGAQRYCEYQDHERSCHTCDMTDPLRSFPARSLTDLNNSNNVTCWRSEPVTGSGDNVTLTLSLGKKFELTYVILQLCPHAPRPDSMVIYKSTDHGLSWQPFQFFSSQCRRLFGRPARQSTGRHNEHEARCSDVTRPLVSRIAFSTLEGRPSSRDLDSSPVLQDWVTATDIRVVFHRLQRPDPQALLSLEAGGATDLASGKYSVPLANGPAGNNIEANLGGDVATSGSGLHYAISDFSVGGRCKCNGHASKCSTDASGQLNCECSHNTAGRDCERCKPFHFDRPWARATAKEANECKECNCNKHARQCRFNMEIFRLSQGVSGGVCQNCRHSTTGRNCHQCKEGFYRDATKPLTHRKVCKACDCHPIGSSGKICNSTSGQCPCKDGVTGLTCNRCARGYQQSRSHIAPCIKQPPRMINMLDTQNTAPEPDEPESSPGSGGDRNGAAGMAAQSQYYRTEGGRECGKCRVSTKRLNLNKFCKRDYAIMAKVIGRDTSSEAVSREVQRRAMDPDVADYEMDQVQPGSARSPITGVYEFQAADYPNPNPNPRGSEMERFDLQIQAVFKRSRPGESSGAGNVYGMPNTTLKRGPMTWIIPTKDLECRCPRIRVNRSYLILGRDSEAPPGYLGIGPHSIVIEWKEDWYRRMKRFQRRARTCA

Target ORF information:

RefSeq Version	NM_001298314.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Netrin-A (NetA), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298314.1

ORF Insert Sequence:

ATGATCCGTG GAATCTTGCT CCTGCTGCTG GGAACCACCA GGTTCAGCCC CATCCAGTGC 
ATCTCCAATG ACGTGTACTT CAAGATGTTT TCGCAACAGG CGCCGCCGGA GGATCCGTGC 
TACAATAAAG CGCACGAGCC ACGCGCCTGC ATTCCGGACT TTGTGAACGC CGCCTACGAT 
GCTCCAGTGG TGGCCAGTTC CACGTGCGGA TCGTCGGGTG CGCAGCGATA TTGCGAGTAC 
CAGGATCACG AGCGCTCCTG CCACACCTGC GACATGACGG ATCCGTTGCG CAGCTTTCCA 
GCCCGCTCGC TGACCGATTT GAATAACTCG AATAATGTGA CCTGCTGGCG CAGTGAGCCG 
GTGACCGGAA GTGGGGACAA CGTAACCTTG ACCCTCTCGC TGGGCAAGAA GTTCGAGCTG 
ACCTATGTGA TCCTACAGCT GTGCCCGCAT GCTCCGCGTC CCGATTCGAT GGTGATCTAC 
AAGAGCACCG ATCACGGGCT CAGTTGGCAG CCATTCCAGT TCTTTAGCTC CCAGTGCCGC 
CGGCTTTTCG GACGTCCCGC CCGCCAATCC ACTGGACGCC ACAATGAGCA CGAGGCCCGC 
TGCAGCGATG TCACCAGGCC ACTGGTCTCC AGAATTGCCT TCAGCACACT GGAGGGACGC 
CCATCGTCAC GGGATCTGGA CTCATCGCCG GTGCTGCAGG ACTGGGTGAC GGCCACCGAT 
ATCCGGGTGG TGTTCCATCG CCTTCAACGC CCCGATCCGC AGGCCCTGCT CTCATTGGAA 
GCCGGAGGAG CAACGGATTT GGCCAGCGGC AAGTACAGTG TGCCCCTGGC CAATGGACCC 
GCTGGCAATA ATATTGAGGC GAATCTCGGT GGGGATGTGG CAACATCCGG CAGTGGATTG 
CACTATGCCA TCTCTGACTT TTCGGTGGGC GGACGGTGCA AGTGCAATGG ACACGCCTCC 
AAGTGTTCCA CGGACGCCAG TGGGCAGCTG AACTGCGAGT GCAGCCACAA TACCGCTGGA 
AGGGATTGCG AACGCTGCAA GCCATTCCAC TTCGATCGTC CATGGGCCAG AGCCACCGCC 
AAGGAAGCCA ATGAGTGCAA AGAGTGCAAC TGCAACAAGC ACGCCCGTCA GTGCCGCTTC 
AATATGGAAA TCTTTCGGCT TTCGCAGGGC GTCTCCGGCG GAGTCTGCCA GAATTGTCGC 
CACTCGACTA CGGGCCGGAA TTGTCACCAG TGCAAGGAGG GATTCTATCG GGACGCCACC 
AAACCACTCA CCCATCGCAA GGTTTGCAAA GCCTGCGATT GCCATCCGAT CGGGTCATCT 
GGCAAGATCT GCAACAGCAC CAGCGGTCAA TGTCCCTGCA AGGACGGGGT CACCGGACTG 
ACCTGCAACC GCTGCGCTCG AGGTTACCAG CAAAGTCGCT CCCACATCGC CCCCTGCATA 
AAACAACCAC CGCGTATGAT CAACATGCTG GACACCCAGA ATACCGCTCC CGAGCCGGAT 
GAGCCCGAAT CCTCTCCAGG ATCTGGTGGC GACCGCAACG GCGCCGCCGG AATGGCCGCC 
CAGTCTCAGT ACTATCGCAC CGAGGGCGGC AGGGAATGCG GCAAATGCAG AGTCAGCACC 
AAAAGGCTAA ACCTAAACAA GTTCTGCAAA AGAGACTACG CAATAATGGC CAAAGTAATT 
GGTCGCGATA CGAGCAGCGA GGCGGTCAGC AGGGAAGTTC AACGGCGCGC AATGGATCCC 
GACGTGGCGG ACTACGAAAT GGATCAGGTG CAGCCGGGAT CGGCCCGATC TCCGATCACG 
GGGGTGTACG AGTTTCAGGC GGCGGACTAT CCCAATCCCA ATCCCAATCC CAGAGGCAGC 
GAAATGGAGC GCTTCGATCT GCAGATCCAG GCCGTCTTCA AGCGCAGCAG GCCAGGCGAA 
AGCAGCGGCG CGGGGAACGT GTACGGAATG CCGAATACAA CCCTAAAACG CGGTCCTATG 
ACCTGGATCA TACCGACTAA GGACCTGGAG TGCCGCTGCC CCAGGATCAG AGTAAACAGA 
TCCTATTTGA TCTTGGGTCG GGACTCGGAG GCGCCACCCG GTTACCTGGG CATCGGACCG 
CACTCGATCG TAATCGAATG GAAGGAGGAC TGGTATCGAC GCATGAAGCG CTTCCAACGG 
AGAGCGCGTA CTTGCGCGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NetA ( NM_001258738.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245667.1 Drosophila melanogaster Netrin-A (NetA), transcript variant B, mRNA.

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CloneID	OFa03562
Clone ID Related Accession (Same CDS sequence)	NM_001258738.2
Accession Version	NM_001258738.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Netrin-A, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001258738.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATCCGTG GAATCTTGCT CCTGCTGCTG GGAACCACCA GGTTCAGCCC CATCCAGTGC 
ATCTCCAATG ACGTGTACTT CAAGATGTTT TCGCAACAGG CGCCGCCGGA GGATCCGTGC 
TACAATAAAG CGCACGAGCC ACGCGCCTGC ATTCCGGACT TTGTGAACGC CGCCTACGAT 
GCTCCAGTGG TGGCCAGTTC CACGTGCGGA TCGTCGGGTG CGCAGCGATA TTGCGAGTAC 
CAGGATCACG AGCGCTCCTG CCACACCTGC GACATGACGG ATCCGTTGCG CAGCTTTCCA 
GCCCGCTCGC TGACCGATTT GAATAACTCG AATAATGTGA CCTGCTGGCG CAGTGAGCCG 
GTGACCGGAA GTGGGGACAA CGTAACCTTG ACCCTCTCGC TGGGCAAGAA GTTCGAGCTG 
ACCTATGTGA TCCTACAGCT GTGCCCGCAT GCTCCGCGTC CCGATTCGAT GGTGATCTAC 
AAGAGCACCG ATCACGGGCT CAGTTGGCAG CCATTCCAGT TCTTTAGCTC CCAGTGCCGC 
CGGCTTTTCG GACGTCCCGC CCGCCAATCC ACTGGACGCC ACAATGAGCA CGAGGCCCGC 
TGCAGCGATG TCACCAGGCC ACTGGTCTCC AGAATTGCCT TCAGCACACT GGAGGGACGC 
CCATCGTCAC GGGATCTGGA CTCATCGCCG GTGCTGCAGG ACTGGGTGAC GGCCACCGAT 
ATCCGGGTGG TGTTCCATCG CCTTCAACGC CCCGATCCGC AGGCCCTGCT CTCATTGGAA 
GCCGGAGGAG CAACGGATTT GGCCAGCGGC AAGTACAGTG TGCCCCTGGC CAATGGACCC 
GCTGGCAATA ATATTGAGGC GAATCTCGGT GGGGATGTGG CAACATCCGG CAGTGGATTG 
CACTATGCCA TCTCTGACTT TTCGGTGGGC GGACGGTGCA AGTGCAATGG ACACGCCTCC 
AAGTGTTCCA CGGACGCCAG TGGGCAGCTG AACTGCGAGT GCAGCCACAA TACCGCTGGA 
AGGGATTGCG AACGCTGCAA GCCATTCCAC TTCGATCGTC CATGGGCCAG AGCCACCGCC 
AAGGAAGCCA ATGAGTGCAA AGAGTGCAAC TGCAACAAGC ACGCCCGTCA GTGCCGCTTC 
AATATGGAAA TCTTTCGGCT TTCGCAGGGC GTCTCCGGCG GAGTCTGCCA GAATTGTCGC 
CACTCGACTA CGGGCCGGAA TTGTCACCAG TGCAAGGAGG GATTCTATCG GGACGCCACC 
AAACCACTCA CCCATCGCAA GGTTTGCAAA GCCTGCGATT GCCATCCGAT CGGGTCATCT 
GGCAAGATCT GCAACAGCAC CAGCGGTCAA TGTCCCTGCA AGGACGGGGT CACCGGACTG 
ACCTGCAACC GCTGCGCTCG AGGTTACCAG CAAAGTCGCT CCCACATCGC CCCCTGCATA 
AAACAACCAC CGCGTATGAT CAACATGCTG GACACCCAGA ATACCGCTCC CGAGCCGGAT 
GAGCCCGAAT CCTCTCCAGG ATCTGGTGGC GACCGCAACG GCGCCGCCGG AATGGCCGCC 
CAGTCTCAGT ACTATCGCAC CGAGGGCGGC AGGGGTAAAA TGCGGCAAAT GCAGAGTCAG 
CACCAAAAGG CTAAACCTAA ACAAGTTCTG CAAAAGAGAC TACGCAATAA TGGCCAAAGT 
AATTGGTCGC GATACGAGCA GCGAGGCGGT CAGCAGGGAA GTTCAACGGC GCGCAATGGA 
TCCCGACGTG GCGGACTACG AAATGGATCA GGTGCAGCCG GGATCGGCCC GATCTCCGAT 
CACGGGGGTG TACGAGTTTC AGGCGGCGGA CTATCCCAAT CCCAATCCCA ATCCCAGAGG 
CAGCGAAATG GAGCGCTTCG ATCTGCAGAT CCAGGCCGTC TTCAAGCGCA GCAGGCCAGG 
CGAAAGCAGC GGCGCGGGGA ACGTGTACGG AATGCCGAAT ACAACCCTAA AACGCGGTCC 
TATGACCTGG ATCATACCGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245667.1
CDS	591..2594
Translation	MIRGILLLLLGTTRFSPIQCISNDVYFKMFSQQAPPEDPCYNKAHEPRACIPDFVNAAYDAPVVASSTCGSSGAQRYCEYQDHERSCHTCDMTDPLRSFPARSLTDLNNSNNVTCWRSEPVTGSGDNVTLTLSLGKKFELTYVILQLCPHAPRPDSMVIYKSTDHGLSWQPFQFFSSQCRRLFGRPARQSTGRHNEHEARCSDVTRPLVSRIAFSTLEGRPSSRDLDSSPVLQDWVTATDIRVVFHRLQRPDPQALLSLEAGGATDLASGKYSVPLANGPAGNNIEANLGGDVATSGSGLHYAISDFSVGGRCKCNGHASKCSTDASGQLNCECSHNTAGRDCERCKPFHFDRPWARATAKEANECKECNCNKHARQCRFNMEIFRLSQGVSGGVCQNCRHSTTGRNCHQCKEGFYRDATKPLTHRKVCKACDCHPIGSSGKICNSTSGQCPCKDGVTGLTCNRCARGYQQSRSHIAPCIKQPPRMINMLDTQNTAPEPDEPESSPGSGGDRNGAAGMAAQSQYYRTEGGRGKMRQMQSQHQKAKPKQVLQKRLRNNGQSNWSRYEQRGGQQGSSTARNGSRRGGLRNGSGAAGIGPISDHGGVRVSGGGLSQSQSQSQRQRNGALRSADPGRLQAQQARRKQRRGERVRNAEYNPKTRSYDLDHTD

Target ORF information:

RefSeq Version	NM_001258738.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Netrin-A (NetA), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258738.2

ORF Insert Sequence:

ATGATCCGTG GAATCTTGCT CCTGCTGCTG GGAACCACCA GGTTCAGCCC CATCCAGTGC 
ATCTCCAATG ACGTGTACTT CAAGATGTTT TCGCAACAGG CGCCGCCGGA GGATCCGTGC 
TACAATAAAG CGCACGAGCC ACGCGCCTGC ATTCCGGACT TTGTGAACGC CGCCTACGAT 
GCTCCAGTGG TGGCCAGTTC CACGTGCGGA TCGTCGGGTG CGCAGCGATA TTGCGAGTAC 
CAGGATCACG AGCGCTCCTG CCACACCTGC GACATGACGG ATCCGTTGCG CAGCTTTCCA 
GCCCGCTCGC TGACCGATTT GAATAACTCG AATAATGTGA CCTGCTGGCG CAGTGAGCCG 
GTGACCGGAA GTGGGGACAA CGTAACCTTG ACCCTCTCGC TGGGCAAGAA GTTCGAGCTG 
ACCTATGTGA TCCTACAGCT GTGCCCGCAT GCTCCGCGTC CCGATTCGAT GGTGATCTAC 
AAGAGCACCG ATCACGGGCT CAGTTGGCAG CCATTCCAGT TCTTTAGCTC CCAGTGCCGC 
CGGCTTTTCG GACGTCCCGC CCGCCAATCC ACTGGACGCC ACAATGAGCA CGAGGCCCGC 
TGCAGCGATG TCACCAGGCC ACTGGTCTCC AGAATTGCCT TCAGCACACT GGAGGGACGC 
CCATCGTCAC GGGATCTGGA CTCATCGCCG GTGCTGCAGG ACTGGGTGAC GGCCACCGAT 
ATCCGGGTGG TGTTCCATCG CCTTCAACGC CCCGATCCGC AGGCCCTGCT CTCATTGGAA 
GCCGGAGGAG CAACGGATTT GGCCAGCGGC AAGTACAGTG TGCCCCTGGC CAATGGACCC 
GCTGGCAATA ATATTGAGGC GAATCTCGGT GGGGATGTGG CAACATCCGG CAGTGGATTG 
CACTATGCCA TCTCTGACTT TTCGGTGGGC GGACGGTGCA AGTGCAATGG ACACGCCTCC 
AAGTGTTCCA CGGACGCCAG TGGGCAGCTG AACTGCGAGT GCAGCCACAA TACCGCTGGA 
AGGGATTGCG AACGCTGCAA GCCATTCCAC TTCGATCGTC CATGGGCCAG AGCCACCGCC 
AAGGAAGCCA ATGAGTGCAA AGAGTGCAAC TGCAACAAGC ACGCCCGTCA GTGCCGCTTC 
AATATGGAAA TCTTTCGGCT TTCGCAGGGC GTCTCCGGCG GAGTCTGCCA GAATTGTCGC 
CACTCGACTA CGGGCCGGAA TTGTCACCAG TGCAAGGAGG GATTCTATCG GGACGCCACC 
AAACCACTCA CCCATCGCAA GGTTTGCAAA GCCTGCGATT GCCATCCGAT CGGGTCATCT 
GGCAAGATCT GCAACAGCAC CAGCGGTCAA TGTCCCTGCA AGGACGGGGT CACCGGACTG 
ACCTGCAACC GCTGCGCTCG AGGTTACCAG CAAAGTCGCT CCCACATCGC CCCCTGCATA 
AAACAACCAC CGCGTATGAT CAACATGCTG GACACCCAGA ATACCGCTCC CGAGCCGGAT 
GAGCCCGAAT CCTCTCCAGG ATCTGGTGGC GACCGCAACG GCGCCGCCGG AATGGCCGCC 
CAGTCTCAGT ACTATCGCAC CGAGGGCGGC AGGGGTAAAA TGCGGCAAAT GCAGAGTCAG 
CACCAAAAGG CTAAACCTAA ACAAGTTCTG CAAAAGAGAC TACGCAATAA TGGCCAAAGT 
AATTGGTCGC GATACGAGCA GCGAGGCGGT CAGCAGGGAA GTTCAACGGC GCGCAATGGA 
TCCCGACGTG GCGGACTACG AAATGGATCA GGTGCAGCCG GGATCGGCCC GATCTCCGAT 
CACGGGGGTG TACGAGTTTC AGGCGGCGGA CTATCCCAAT CCCAATCCCA ATCCCAGAGG 
CAGCGAAATG GAGCGCTTCG ATCTGCAGAT CCAGGCCGTC TTCAAGCGCA GCAGGCCAGG 
CGAAAGCAGC GGCGCGGGGA ACGTGTACGG AATGCCGAAT ACAACCCTAA AACGCGGTCC 
TATGACCTGG ATCATACCGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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NetA cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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