DNAlig4 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	DNAlig4
Entrez Gene ID	32322
Full Name	Ligase4
Synonyms	CG12176,Dmel\CG12176,RE37186,lig4,ligIV,ligaseIV
General protein information	Preferred Names Ligase4 Names CG12176 gene product from transcript CG12176-RA CG12176-PA DNA ligase 4 DNA ligase IV Lig4-PA ligase 4 ligase IV
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 12B2-12B2

mRNA and Protein(s)

mRNA	Protein	Name
NM_132679.3	NP_572907.1	DNA ligase 4

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Lig4	NP_001099565.1
Drosophila melanogaster (fruit fly)	Lig4	NP_572907.1
Magnaporthe oryzae (rice blast fungus)	MGG_12899	XP_003712808.1
Bos taurus (cattle)	LIG4	NP_001178055.1
Saccharomyces cerevisiae (baker's yeast)	DNL4	NP_014647.1
Eremothecium gossypii	AGOS_ACR008W	NP_983411.1
Gallus gallus (chicken)	LIG4	NP_001025987.1
Kluyveromyces lactis	KLLA0D01089g	XP_453119.1
Neurospora crassa	NCU06264	XP_962868.1
Mus musculus (house mouse)	Lig4	NP_795927.2
Danio rerio (zebrafish)	lig4	NP_001096593.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000623	XP_310455.4
Arabidopsis thaliana (thale cress)	ATLIG4	NP_568851.2
Homo sapiens (human)	LIG4	NP_001091738.1
Pan troglodytes (chimpanzee)	LIG4	XP_003314254.1
Canis lupus familiaris (dog)	LIG4	XP_005634152.1
Schizosaccharomyces pombe (fission yeast)	lig4	NP_587888.2
Xenopus tropicalis (tropical clawed frog)	lig4	NP_001016981.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that when both spnA and lig4 were mutated and a circular donor was provided, the frequency of ry mutations was still high and no HR products were recovered.
Title: Genetic analysis of zinc-finger nuclease-induced gene targeting in Drosophila.

Lig4 is required for repair of DNA double-strand breaks induced by P-element excision.
Title: Lig4 and rad54 are required for repair of DNA double-strand breaks induced by P-element excision in Drosophila.

The following DNAlig4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DNAlig4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa01526	NM_132679.3 Latest version!	Drosophila melanogaster DNA ligase 4 (DNAlig4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DNAlig4 ( NM_132679.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572907.1 Drosophila melanogaster DNA ligase 4 (DNAlig4), mRNA.

Price & Availability↑

CloneID	OFa01526
Clone ID Related Accession (Same CDS sequence)	NM_132679.3
Accession Version	NM_132679.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	DNA ligase 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_132679.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGTAG ATATAGCCAG CACGATCAAG TTCCGGGATA TTTGCAGCCT GTTCGAGAAG 
ATTAAGGCCA CGCAAAAGGT GGCTAACAAG GAGGAGGTCC TCAAGAGCTA CTACGAGTCC 
TTCTGTCGCC ACCGCGAATC CTTTCGCCGG CAAACCGGAT TAAACAACGA CCAGCCGGAG 
GACGGTGCCA GTAGCTTCTA TTCCGTGCTG CGACTGCTGC TTCCGGGTGC GGACACTGGT 
CGGGATACCT ATGGATTGCA GATCACCGCG CTGGGCAGGC TCTACATCAG GGTGCTCCAG 
CTACCCACGG ACTCCAGCGA TGCCATCAGG CTGCAGCACC GCAACGGAAA CATGTACCGG 
GACTACGGTG ATGTTGTTTA TTCCGTGCTT AAGCCAAGGT GCTTTAATCC GCCCAGCAAT 
TTGAGACTGA AAGAAATCCA TCAGATGCTG GACACCATTG CCAACGAGGA CACAGAAGTT 
AAGCAGCAGC AACTCATCCG GTTTACGGAG CAGGCCTCGC CTGAGGAACA GAAGTGGCTA 
ATTCGGTTGC TGCTTAAGAG CCTCGGGCTG GGGATCGGTG AGCAAAAGAT TTTCGGTGTG 
CTGCACCCCA AGGCGCAGGA TATCTACCAG CGCTGCTCCG ATCTGGGACA TGTGTGCAAT 
CTGCTGGCAG ACCGAACCAC CGACCTAGAC GCCAGCAGCA GCAAGGACAG CAAGGCGGCG 
GTCAAGTTTG TAAACCTGAA TTCCGTCATA CGGCCATTCC ACCAGATACG TCCCATGCTG 
TGCGAACGAT TCCCCGGTGA CATCCAGGAG CTGATGCAGT CGGATGTGCT CTATCTGGAG 
ACCAAAATGG ATGGCGAACG CTTTCAGCTG CACATCGATC GGGGTCGTTT CATGTACATT 
TCCCGCAATG GAGTGGACTA CACCAGAAAC TTTGGTCACA GCTACGATCA CGGCACCTTA 
ACGCCAAAGC TGAGGGGACT TCTGCCGCTG GGTCTGGAGT CTATCATACT CGACGGCGAG 
ATGATGGTGT GGGATACCAA CAAACTGCGC TTTCGGGAAA AAGGCGAGAA TACGGACGTG 
AAGAGCCTGA AGCCGGAGGG CAGCTGGCAG CCATGTTTCG TGGTGTACGA TCTGCTATAT 
TTTAATGGCC AGAGTCTGCT GGACCATACC TACATTCAGC GGGCGTACAA ATTGCAGAAA 
CTCATAGTTG AACAGTCGGG TGTGCTGCAG TTGATGCGTG CACGCAAAAT CGGTTCCGTT 
CAAGAGTTCA ACGAGCTGTT CCAGCAGGCA CTCGACTCCC ACGCCGAGGG CATTGTGCTA 
AAGAAGCAGG GATCAAGATA TCAGCCTGGC GTTAGACTGG GCGGTGGGTG GTACAAGGAC 
AAGGCTGATT ACATCAAAGG GCTGATCACC GAGTTCGATG TGCTGATCAT CGGGGCATTC 
TACAATCGCA AGCGCACCTT TGTGGACTCA TTTTTACTGG GCGTTCTCCA GCCGGCGCCG 
CCAGGCAGCT CTAATCGTCC CGAAGTCTTC AGTATTGGAG TGGTTGCCAA CAATACGAAA 
CAGCGCGGAG TGCTAAATCA CACACTGAAA CCACATTGGC ATGATGTAGT CAATGAGCCA 
CCGCCATTGT GGTTTCACTA CAAGCCCAAG GAGAGGTCCG GATGCCCCGA TCTGTGGATT 
GAACCGCAGA ACTCGGTAAT TTTGCAGGTG AAAGCTGCTG ATCTGGCGCC CAATGGAGCA 
TTTTTCACCC GAAAATCGTT GCACTTTCCG CGCACTGAGA TGAAACGGGA TGATAAGACC 
TGGAGCGAGT GTATGACACT TAAGGAGTTT AACGATCTCT GCGGAGGTCC TTTGGCCATC 
AAGAAGTTAA ACAAGCGGCA ACTGCGACTA GAGGATGTGA CCACCAAGCG GAAACAGATG 
CGTATGACGC CCTCGGAGCG AAGCCGACTG GGATTGGCCG TCTATGAGAA GCGCTATGAC 
GCTAGCACCT CCGCTAGCAC TTCCAAACTT TTCGACGGTC TGAGCTTTTG CATTCTGAGC 
GGATCTGCCG GACGACACAG CAAGCATCAG CTGCAGGAGC TGGCTGTTAA AAACGGTGGC 
TGCATCGTGG AAAATCCCCT GCCCAATGAT CCAAAGTGTT TTTGCATCGC AGGCGATGAG 
ACGTTTTTAG TTAAGCGGCT CATCCTTCAA CAGCCACGCA CCTGTGACAT TGTGCGCATG 
GAGTGGCTAC TTAGGGTCTG TCAGAAGCAG GAGCTTGAAC TAAAGCCTAG AGATCTTATT 
GCGGCCACTG AACCACTGCA GCAGGATCTG GCTGAATGTT TTGATCGGCA CGGTGATAGC 
TACACCAAAG ACATTGCCAA CGTGGAGGAG CTGCAGGATC TGCTTCAAGG CATTGAGTTG 
ACAGCTGATA ATGTGGCTGG CATAACCGCA TCTAATCTCA ATGCTCTGGA GGATCAGCTT 
CTGGATGGGA AAAAGAATCT GAACATGTTC CGCAACCTTA ATGCGTTCTT CTACAGTCCG 
CATGGTGATG AAGTGGCTAA GCTTCTGTTT CTTCAAAACG GCGGCCGGAT AGTCGACGAT 
TCCGATCCGC AACTAAATCT GGGATTTATC TGTATGTCTT CTGACATCGA TAACGACCAC 
TTCGAGCACT GGCTGCACAA TCATTCCAAG TTGACCACTG ACAAGGTCTT AAACTCGGCG 
TGGATACATC AGTGCCATCG CGAAGGCATC CTACTTCCTA TGCATTCTTT CGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572907.1
CDS	72..2828
Translation	MSVDIASTIKFRDICSLFEKIKATQKVANKEEVLKSYYESFCRHRESFRRQTGLNNDQPEDGASSFYSVLRLLLPGADTGRDTYGLQITALGRLYIRVLQLPTDSSDAIRLQHRNGNMYRDYGDVVYSVLKPRCFNPPSNLRLKEIHQMLDTIANEDTEVKQQQLIRFTEQASPEEQKWLIRLLLKSLGLGIGEQKIFGVLHPKAQDIYQRCSDLGHVCNLLADRTTDLDASSSKDSKAAVKFVNLNSVIRPFHQIRPMLCERFPGDIQELMQSDVLYLETKMDGERFQLHIDRGRFMYISRNGVDYTRNFGHSYDHGTLTPKLRGLLPLGLESIILDGEMMVWDTNKLRFREKGENTDVKSLKPEGSWQPCFVVYDLLYFNGQSLLDHTYIQRAYKLQKLIVEQSGVLQLMRARKIGSVQEFNELFQQALDSHAEGIVLKKQGSRYQPGVRLGGGWYKDKADYIKGLITEFDVLIIGAFYNRKRTFVDSFLLGVLQPAPPGSSNRPEVFSIGVVANNTKQRGVLNHTLKPHWHDVVNEPPPLWFHYKPKERSGCPDLWIEPQNSVILQVKAADLAPNGAFFTRKSLHFPRTEMKRDDKTWSECMTLKEFNDLCGGPLAIKKLNKRQLRLEDVTTKRKQMRMTPSERSRLGLAVYEKRYDASTSASTSKLFDGLSFCILSGSAGRHSKHQLQELAVKNGGCIVENPLPNDPKCFCIAGDETFLVKRLILQQPRTCDIVRMEWLLRVCQKQELELKPRDLIAATEPLQQDLAECFDRHGDSYTKDIANVEELQDLLQGIELTADNVAGITASNLNALEDQLLDGKKNLNMFRNLNAFFYSPHGDEVAKLLFLQNGGRIVDDSDPQLNLGFICMSSDIDNDHFEHWLHNHSKLTTDKVLNSAWIHQCHREGILLPMHSFV

Target ORF information:

RefSeq Version	NM_132679.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster DNA ligase 4 (DNAlig4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132679.3

ORF Insert Sequence:

ATGAGCGTAG ATATAGCCAG CACGATCAAG TTCCGGGATA TTTGCAGCCT GTTCGAGAAG 
ATTAAGGCCA CGCAAAAGGT GGCTAACAAG GAGGAGGTCC TCAAGAGCTA CTACGAGTCC 
TTCTGTCGCC ACCGCGAATC CTTTCGCCGG CAAACCGGAT TAAACAACGA CCAGCCGGAG 
GACGGTGCCA GTAGCTTCTA TTCCGTGCTG CGACTGCTGC TTCCGGGTGC GGACACTGGT 
CGGGATACCT ATGGATTGCA GATCACCGCG CTGGGCAGGC TCTACATCAG GGTGCTCCAG 
CTACCCACGG ACTCCAGCGA TGCCATCAGG CTGCAGCACC GCAACGGAAA CATGTACCGG 
GACTACGGTG ATGTTGTTTA TTCCGTGCTT AAGCCAAGGT GCTTTAATCC GCCCAGCAAT 
TTGAGACTGA AAGAAATCCA TCAGATGCTG GACACCATTG CCAACGAGGA CACAGAAGTT 
AAGCAGCAGC AACTCATCCG GTTTACGGAG CAGGCCTCGC CTGAGGAACA GAAGTGGCTA 
ATTCGGTTGC TGCTTAAGAG CCTCGGGCTG GGGATCGGTG AGCAAAAGAT TTTCGGTGTG 
CTGCACCCCA AGGCGCAGGA TATCTACCAG CGCTGCTCCG ATCTGGGACA TGTGTGCAAT 
CTGCTGGCAG ACCGAACCAC CGACCTAGAC GCCAGCAGCA GCAAGGACAG CAAGGCGGCG 
GTCAAGTTTG TAAACCTGAA TTCCGTCATA CGGCCATTCC ACCAGATACG TCCCATGCTG 
TGCGAACGAT TCCCCGGTGA CATCCAGGAG CTGATGCAGT CGGATGTGCT CTATCTGGAG 
ACCAAAATGG ATGGCGAACG CTTTCAGCTG CACATCGATC GGGGTCGTTT CATGTACATT 
TCCCGCAATG GAGTGGACTA CACCAGAAAC TTTGGTCACA GCTACGATCA CGGCACCTTA 
ACGCCAAAGC TGAGGGGACT TCTGCCGCTG GGTCTGGAGT CTATCATACT CGACGGCGAG 
ATGATGGTGT GGGATACCAA CAAACTGCGC TTTCGGGAAA AAGGCGAGAA TACGGACGTG 
AAGAGCCTGA AGCCGGAGGG CAGCTGGCAG CCATGTTTCG TGGTGTACGA TCTGCTATAT 
TTTAATGGCC AGAGTCTGCT GGACCATACC TACATTCAGC GGGCGTACAA ATTGCAGAAA 
CTCATAGTTG AACAGTCGGG TGTGCTGCAG TTGATGCGTG CACGCAAAAT CGGTTCCGTT 
CAAGAGTTCA ACGAGCTGTT CCAGCAGGCA CTCGACTCCC ACGCCGAGGG CATTGTGCTA 
AAGAAGCAGG GATCAAGATA TCAGCCTGGC GTTAGACTGG GCGGTGGGTG GTACAAGGAC 
AAGGCTGATT ACATCAAAGG GCTGATCACC GAGTTCGATG TGCTGATCAT CGGGGCATTC 
TACAATCGCA AGCGCACCTT TGTGGACTCA TTTTTACTGG GCGTTCTCCA GCCGGCGCCG 
CCAGGCAGCT CTAATCGTCC CGAAGTCTTC AGTATTGGAG TGGTTGCCAA CAATACGAAA 
CAGCGCGGAG TGCTAAATCA CACACTGAAA CCACATTGGC ATGATGTAGT CAATGAGCCA 
CCGCCATTGT GGTTTCACTA CAAGCCCAAG GAGAGGTCCG GATGCCCCGA TCTGTGGATT 
GAACCGCAGA ACTCGGTAAT TTTGCAGGTG AAAGCTGCTG ATCTGGCGCC CAATGGAGCA 
TTTTTCACCC GAAAATCGTT GCACTTTCCG CGCACTGAGA TGAAACGGGA TGATAAGACC 
TGGAGCGAGT GTATGACACT TAAGGAGTTT AACGATCTCT GCGGAGGTCC TTTGGCCATC 
AAGAAGTTAA ACAAGCGGCA ACTGCGACTA GAGGATGTGA CCACCAAGCG GAAACAGATG 
CGTATGACGC CCTCGGAGCG AAGCCGACTG GGATTGGCCG TCTATGAGAA GCGCTATGAC 
GCTAGCACCT CCGCTAGCAC TTCCAAACTT TTCGACGGTC TGAGCTTTTG CATTCTGAGC 
GGATCTGCCG GACGACACAG CAAGCATCAG CTGCAGGAGC TGGCTGTTAA AAACGGTGGC 
TGCATCGTGG AAAATCCCCT GCCCAATGAT CCAAAGTGTT TTTGCATCGC AGGCGATGAG 
ACGTTTTTAG TTAAGCGGCT CATCCTTCAA CAGCCACGCA CCTGTGACAT TGTGCGCATG 
GAGTGGCTAC TTAGGGTCTG TCAGAAGCAG GAGCTTGAAC TAAAGCCTAG AGATCTTATT 
GCGGCCACTG AACCACTGCA GCAGGATCTG GCTGAATGTT TTGATCGGCA CGGTGATAGC 
TACACCAAAG ACATTGCCAA CGTGGAGGAG CTGCAGGATC TGCTTCAAGG CATTGAGTTG 
ACAGCTGATA ATGTGGCTGG CATAACCGCA TCTAATCTCA ATGCTCTGGA GGATCAGCTT 
CTGGATGGGA AAAAGAATCT GAACATGTTC CGCAACCTTA ATGCGTTCTT CTACAGTCCG 
CATGGTGATG AAGTGGCTAA GCTTCTGTTT CTTCAAAACG GCGGCCGGAT AGTCGACGAT 
TCCGATCCGC AACTAAATCT GGGATTTATC TGTATGTCTT CTGACATCGA TAACGACCAC 
TTCGAGCACT GGCTGCACAA TCATTCCAAG TTGACCACTG ACAAGGTCTT AAACTCGGCG 
TGGATACATC AGTGCCATCG CGAAGGCATC CTACTTCCTA TGCATTCTTT CGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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DNAlig4 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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